CN201413292Y - Protein quick detecting system - Google Patents
Protein quick detecting system Download PDFInfo
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- CN201413292Y CN201413292Y CN2009201568115U CN200920156811U CN201413292Y CN 201413292 Y CN201413292 Y CN 201413292Y CN 2009201568115 U CN2009201568115 U CN 2009201568115U CN 200920156811 U CN200920156811 U CN 200920156811U CN 201413292 Y CN201413292 Y CN 201413292Y
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- colorimetric pool
- stirrer
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Abstract
The utility model relates to a protein quick detecting system which comprises an automatic detecting device formed by a main detecting machine and a sample weighing device; the protein quick detectingsystem is characterized by further comprising an automatic sample feeding device which comprises a peristaltic pump, a liquid inlet end of which is sequentially communicated with a centrifuge tube filled with solution to be detected, and a circulating cuvette sheathed inside a circulating colorimetric pond, and a liquid outlet end thereof is communicated with a waste liquid bottle; and the circulating colorimetric pond is arranged inside the main detecting machine and is provided with a light source monochromator and a photoelectric detector which are electrically connected with the main detecting machine. The protein quick detecting system can be used for quickly detecting the real content of protein in the detected samples, including dairy produce, processed meat, raw meat, grain, pet food and the like.
Description
Technical field
The utility model belongs to field of analytic instrument, relates to the analytical instrument system of protein content in a kind of easy, sensitive, quick on-the-spot test sample, is specifically related to a kind of rapid detection apparatus that is used for fast-field evaluation sample protein real content.
Background technology
Protein mainly contains casein, lactalbumin, immunoglobulin (Ig) etc.Proteinaceous nutrient is abundant, digests and assimilates easily.The protein that all contains high level in dairy products and other based foods.Because interests drive, some illegal people make an issue of on proteinaceous industrial chain, add some materials by various means and pretend to be protein, and their product is got by under false pretences in general protein detection instrument, and eater's health is damaged.Therefore, it is very necessary true Protein content in the food being detected.In the protein detection instrument in food, full-automatic phi protein is arranged now abroad, but the price comparison costliness, and can only detect a sample at every turn.
Summary of the invention
At the problems referred to above, fundamental purpose of the present utility model provide a kind of can be easy, quick, on-the-spot, the rapid detection system of protein real content in the detection by quantitative sample.
For achieving the above object, the utility model provides a kind of protein rapid detection system, comprise by detecting the automatic detection device that main frame and sample weighing device are formed, also comprise an automatic sampling apparatus, described automatic sampling apparatus comprises a peristaltic pump, its liquid feeding end is communicated with centrifuge tube that solution to be checked is housed and the circulation cuvette that is sleeved in the circulation colorimetric pool successively, outlet end is communicated with a waste liquid bottle, described circulation colorimetric pool places and detects in the main frame, establishes on it and detects light source monochromator and the photoelectric detector that main frame is electrically connected.
Wherein, described stream circulation cuvette has an inside groove, and two ends have injection port to be connected with silicone tube respectively with outlet; Circulation cuvette fixed cover is placed in the circulation colorimetric pool, and its Outside Dimensions matches with the circulation colorimetric pool; The circulation colorimetric pool has upper port and lower port, and size matches with circulation cuvette injection port and outlet; Circulation colorimetric pool side corresponding horizontal level is established two and is connected perforates, the superelevation amount light emitting diode that one of them through hole installing had not only been done light source but also made monochromator, and another connects in perforate and installs photoelectric detector.
In this protein rapid detection system, also comprise a stirring apparatus, described stirring apparatus comprises stirrer, stirrer frame, jar and stirring cup; Described stirrer frame is a metal ring carriage, and stirrer is put on top, and the stirring cup is put in the bottom; Described stirrer is the portable rechargeable stirrer, has and the USB interface that detects the main frame two-way communication.
This protein rapid detection system also comprises an automatic liquid getting device, and liquid getting device has and the USB interface that detects the main frame two-way communication automatically.
Described sample weighing device is the standard analysis balance, and it is provided with and detects the data-interface of main frame two-way communication.
Described detection main frame has a casing, the front surface of casing is provided with LCDs and a plurality of operating key, casing upper surface is provided with the colorimetric pool lid and prints outlet, and the casing side-lower is provided with supply socket and power control switch, and the casing side also is provided with a plurality of data-interfaces; Peristaltic pump, circulation colorimetric pool and circulation cuvette in the inherent colorimetric pool lid of the casing lower space installing automatic sampling apparatus, a microprocessor is installed in other space in the casing, and this microprocessor is electrically connected with power supply, power control switch, light source, photoelectric detector, operating key, printer, display screen and data-interface.
Described data-interface is RS-232 interface or USB interface.
The utility model milk protein detection system is made of sample pre-treatments device, automatic sampling apparatus, automatic detection device, can detect a plurality of samples simultaneously.Protein content in can the fast detecting sample.Comprise dairy products, manufactured meat, raw meat, cereal and pet food etc.As: Protein content in former breast, plain chocolate, colostrum, skimmed milk power, non-skimmed milk power, baby milk powder, coffee milk, walnut milk, chocolate milk, egg, ice cream, cheese, beef hot dog, pure beef intestines, pure pork intestines, turkey, can meat, coarse food grain and the flour.
The protein detection system distinguishing feature that the utility model provides is:
1, adopts sample pre-treatments device, automatic sampling apparatus, automatic detection device, have USB or RS232 interface and detect main-machine communication, automaticity height.
2, it be basic adopting the colorimetric analysis method, makes easy and simple to handlely, and the testing result intuitive is good.
3, adopt Single-chip Controlling, have measurement, setting, record, preservation and data processing function.
Description of drawings
Fig. 1 constitutes synoptic diagram for the utility model.
Fig. 2-1 detects the main machine structure synoptic diagram for the utility model.
Fig. 2-2 is the utility model sample weighing apparatus structure synoptic diagram.
Fig. 3-1 is the utility model stirring apparatus structural representation.
Fig. 3-2 is the utility model stirrer shelf structure synoptic diagram.
Fig. 3-3 is the utility model agitator structure synoptic diagram.
Fig. 3-4 is the utility model liquid taking device structural representation.
Fig. 4-1 is the utility model automatic sampling apparatus structural representation.
Fig. 4-2 is the utility model circulation cuvette structural representation.
Fig. 4-3 is the utility model circulation colorimetric pool structural representation.
Embodiment
Below in conjunction with description of drawings embodiment of the present utility model.
The protein rapid detection system that the utility model proposes is complete sample preparation and the instrumental analysis system that sets up on the basis of colorimetric method.
The utility model provides the protein detection system, and it comprises several parts of sample pre-treatments device, automatic sampling apparatus and automatic detection device.Referring to shown in Figure 1, described sample pre-treatments device comprises liquid taking device 34 (referring to Fig. 3-4) and stirring apparatus 3 (referring to Fig. 3-1); Described automatic sampling apparatus 4 comprises centrifuge tube 42, circulation colorimetric pool 44, circulation cuvette 45, peristaltic pump 47 and the waste liquid bottle 41 (referring to Fig. 4-1) that is interconnected; Described automatic detection device comprises detection main frame 1 and the sample weighing device 2 that signal connects.Here, the circulation colorimetric pool 44 of automatic sampling apparatus 4, circulation cuvette 45 and peristaltic pump 47 place in the detection main frame 1 of automatic detection device, and the stirring apparatus 3 of sample pre-treatments device is connected with detection main frame 1 signal of automatic detection device.
Shown in Fig. 2-1, detecting main frame 1 in the utility model has a casing 11, and the front surface of casing 11 is provided with LCDs 15, a plurality of operating key 16 (as return key, acknowledgement key, upturning key, downturning key).Casing 11 upper surfaces are provided with colorimetric pool lid 14 and print outlet 13, and casing 11 side-lowers are provided with supply socket and power control switch (not shown), and casing 11 sides also are provided with a plurality of data-interfaces (RS-232 interface or USB interface) 12; Casing 11 inherent colorimetric pools cover peristaltic pump, circulation colorimetric pool (band light source and photoelectric detector) and the circulation cuvette in the 14 lower space installing automatic sampling apparatus, a microprocessor (not drawing among the figure) is installed in other space in the casing 11, this microprocessor is electrically connected in the usual way with power supply, power control switch, light source, photoelectric detector, operating key 16, printer, display screen 15 and data-interface 12, and is connected with sample pre-treatments device 3 signals with sample weighing device 2 by corresponding data interface 12 (RS-232 interface or USB interface).
Shown in Fig. 2-2, the utility model sample weighing device 2 has analytical balance main body 21, balance on goods and services 22, balance hurricane globe 23, day plug door 24, sliding door handle 25 and power switch, supply socket (omitting among the figure) and RS232/USB interface 28, liquid crystal display 26, a plurality of button 27 (for example return key, acknowledgement key, upturning key and downturning key).The utility model sample weighing device is the standard analysis balance, can be the BL120S precision balance in the himalaya series of setra company, pinpoint accuracy, high resolving power, response fast, detects in real time.The RS232/USB interface 28 of sample weighing device and the data-interface of main frame 1 12 are connected by data line.
Described sample pre-treatments device comprises liquid taking device 34 and stirring apparatus 3.Shown in Fig. 3-1, the utility model stirring apparatus 3 has a jar 31, and wherein, jar 31 is disposable 200mL plastic cup, and detected sample is put into the cup mixing.When sample detection, disposable use is avoided polluting and is with low cost.Stirrer 33 is placed on the stirrer frame 32.
Shown in Fig. 3-3, stirrer 33 is the portable rechargeable stirrer, can hand, and also can be placed on operation on the stirrer frame 32, and is easy to use.Stirrer 33 comprises a main body 331, on main body 331 tops one USB interface 332 and supply hub 333 are arranged, USB interface 332 and data-interface 12 two-way communications that detect main frame, supply hub 333 can also can be agitator body 331 inner lithium cell chargings with external power supply, 334 is power switch, and 335 is stirrer.
The utility model can dispose a liquid taking device 34 in addition, as shown in Figure 3-4, liquid taking device 34 has a reagent bottle 341, reagent bottle is 2 liters of wide-mouth plastic bottles, the inside required reagent when sample detection is housed, described liquid getting device 343 is the 50mL plastic injector for temporary use, is used to extract the reagent in the reagent bottle.In use, using liquid getting device 343 to extract required quantitative reagent by testing requirements then for 341 li from reagent bottle cap insertion reagent bottle at syringe needle 342 gets final product.Liquid getting device 343 can use the 27G syringe available from Shenzhen Xin Laige Science and Technology Ltd..
The volumetric soiutions that takes out is packed into behind the centrifuge tube 42, with centrifuge tube 42 put into hydro-extractor centrifugal, separate, centrifuge tube 42 is taken out put into automatic detection device 4 then.Hydro-extractor is used for the sample centrifuging, and is can a multitube centrifugal, promptly can be used for carrying out a plurality of sample detection this moment.The hydro-extractor function is not described further at this.
Shown in Fig. 4-1, be provided with a peristaltic pump 47 in the automatic sampling apparatus 4, be the standard specialty products, its liquid feeding end is communicated with a circulation cuvette 45 and a centrifuge tube 42 successively by silicone tube 43, solution to be detected after centrifugal is housed in the centrifuge tube 42, circulation cuvette 45 places the circulation colorimetric pool 44 that light source and photoelectric detector are housed, and the outlet end of peristaltic pump 47 is communicated with a waste liquid bottle 41 by silicone tube 46.
Continuation is referring to Fig. 4-2, and circulation cuvette 45 is a standard specialty cuvette, has an inside groove 453, lower end injection port 451 feed liquors, and outlet 452 fluids in upper end are connected with silicone tube 43 respectively.Circulation cuvette 45 fixedly is nested with in circulation colorimetric pool 44, and its Outside Dimensions matches with circulation colorimetric pool 414.Continuation is referring to Fig. 4-3, and circulation colorimetric pool 44 has upper port 443 and lower port 445, and size matches with circulation cuvette 45 injection ports 451 and outlet 452; The corresponding horizontal level in circulation colorimetric pool 44 sides is established and is connected perforate 442 and 444.Wherein, installing superelevation amount light emitting diode is not only done light source but also make monochromator in the through hole 442.In the present embodiment, adopted the Japanese super bright light emitting diode of 470nm that day is inferior and Toyota Company produces, luminance parameter is more than 1000mcd.Place photoelectric detector in another through hole 444.Photodetector unit has adopted American TI Company to produce the integrated electro sensor, and model is TSL230.Certainly, the utility model also can adopt other to have the integrated electro sensor of equal effect.
The utility model course of work: after being communicated with power supply, open sample weighing device 2, weighing numerical value imports in the microprocessor by data-interface 12, then the sample after the weighing being put into stirring apparatus 3 stirs, by liquid taking device 34 getting reagent is injected stirring apparatus 3, microprocessor stirs by data-interface 12 control stirring apparatus motors, and then by liquid taking device 34 get quantitative mixed solution put into centrifugal to hydro-extractor behind the centrifuge tube, separate, getting the liquid quantitative value can import in the microprocessor by data-interface; Centrifuge tube is put into automatic sampling apparatus 4 from the hydro-extractor taking-up, open peristaltic pump, the monochromatic light that the light source/monochromator of circulation on the colorimetric pool 44 sends shines photoelectric detector after passing sample solution in the circulation cuvette, after photoelectric detector is converted to electric signal with detected light signal, be transferred to microprocessor, carry out data analysis by microprocessor and the result is outputed to display screen 15 or pass through printer 13 outputs.Microprocessor is the ARM series that PHILIPS Co. produces, and its major parameter is 32 single-chip microcomputers, internal storage 40K.But the utility model with light source/monochromator, photoelectric detector and microprocessor be the circuit of the spectrophotometric analysis instrument of core parts be connected and course of work application reference people before the introduction of ZL200620004295.0, do not repeat them here.Other circuit connection is custom circuit.
Introduce the using method of the utility model protein detection system below in conjunction with concrete operations.
One, sample pre-treatments
1, milk powder sample preparation
Operation detects main frame, takes by weighing milk powder 5g with the BL120S precision balance and puts into jar (beaker), injects the 50mL pure water to jar again, jar is placed on stirs on the cup then, stirrer is inserted stirrer frame seat of honour square opening began to stir mixing 2 minutes.
Another jar is put into the BL120S precision balance, with the weighing 1g milk solution in the jar of BL120S precision balance of the milk solution behind the suction pipe absorption mixing, jar is taken out, get 40mL reagent with liquid getting device and inject the jar that 1g milk solution is housed, weighing, stirred mixing after the taking-up again 2 minutes.Get solution behind the 5mL mixing then in the 5mL centrifuge tube, hydro-extractor is centrifugal, separated 3 minutes.Stand-by.
2, liquid milk sample preparation
Draw the weighing in the jar of BL120S precision balance of 0.5mL milk with suction pipe, get 40mL reagent with liquid getting device then and inject above-mentioned jar, mixing was stirred in weighing again 2 minutes after the taking-up.Get solution behind the 5mL mixing then in the 5mL centrifuge tube, hydro-extractor is centrifugal, separated 3 minutes.Stand-by.
Two, sample detection
Stand-by solution in the 5mL centrifuge tube is inserted in the automatic sampling apparatus, operate by detecting the main frame prompting, can detect protein content in the sample, testing result shows on LCDs automatically.
Claims (7)
1, a kind of protein rapid detection system, comprise by detecting the automatic detection device that main frame and sample weighing device are formed, it is characterized in that, also comprise an automatic sampling apparatus, described automatic sampling apparatus comprises a peristaltic pump, its liquid feeding end is communicated with centrifuge tube that solution to be checked is housed and the circulation cuvette that is sleeved in the circulation colorimetric pool successively, outlet end is communicated with a waste liquid bottle, described circulation colorimetric pool places and detects in the main frame, establishes on it and detects light source monochromator and the photoelectric detector that main frame is electrically connected.
According to the described protein rapid detection system of claim 1, it is characterized in that 2, described stream circulation cuvette has an inside groove, two ends have injection port to be connected with silicone tube respectively with outlet; Circulation cuvette fixed cover is placed in the circulation colorimetric pool, and its Outside Dimensions matches with the circulation colorimetric pool; The circulation colorimetric pool has upper port and lower port, and size matches with circulation cuvette injection port and outlet; Circulation colorimetric pool side corresponding horizontal level is established two and is connected perforates, the superelevation amount light emitting diode that one of them through hole installing had not only been done light source but also made monochromator, and another connects in perforate and installs photoelectric detector.
According to claim 1 or 2 described protein rapid detection system, it is characterized in that 3, also comprise a stirring apparatus, described stirring apparatus comprises stirrer, stirrer frame, jar and stirring cup; Described stirrer frame is a metal ring carriage, and stirrer is put on top, and the stirring cup is put in the bottom; Described stirrer is the portable rechargeable stirrer, has and the USB interface that detects the main frame two-way communication.
4, according to the described protein rapid detection system of claim 3, it is characterized in that, also comprise an automatic liquid getting device, liquid getting device has and the USB interface that detects the main frame two-way communication automatically.
5, according to claim 1 or 2 described protein rapid detection system, it is characterized in that described sample weighing device is the standard analysis balance, it is provided with and detects the data-interface of main frame two-way communication.
6, according to claim 1 or 2 described protein rapid detection system, it is characterized in that, described detection main frame has a casing, the front surface of casing is provided with LCDs and a plurality of operating key, casing upper surface is provided with the colorimetric pool lid and prints outlet, the casing side-lower is provided with supply socket and power control switch, and the casing side also is provided with a plurality of data-interfaces; Peristaltic pump, circulation colorimetric pool and circulation cuvette in the inherent colorimetric pool lid of the casing lower space installing automatic sampling apparatus, a microprocessor is installed in other space in the casing, and this microprocessor is electrically connected with power supply, power control switch, light source, photoelectric detector, operating key, printer, display screen and data-interface.
According to the described protein rapid detection system of claim 6, it is characterized in that 7, described data-interface is RS-232 interface or USB interface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201568115U CN201413292Y (en) | 2009-05-26 | 2009-05-26 | Protein quick detecting system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201568115U CN201413292Y (en) | 2009-05-26 | 2009-05-26 | Protein quick detecting system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201413292Y true CN201413292Y (en) | 2010-02-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009201568115U Expired - Lifetime CN201413292Y (en) | 2009-05-26 | 2009-05-26 | Protein quick detecting system |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102486447A (en) * | 2010-12-06 | 2012-06-06 | 沈阳理工大学 | Rapid detection device of milk components |
| CN106248603A (en) * | 2016-09-27 | 2016-12-21 | 济南恒辉科济食品配料有限公司 | Food is suspended and opaque system Stability Determination detection method and detection device |
| CN107966433A (en) * | 2016-10-19 | 2018-04-27 | 姬志刚 | A kind of dateplum persimmon protein rapid detection apparatus |
| CN110487764A (en) * | 2019-08-30 | 2019-11-22 | 天津陆海石油设备系统工程有限责任公司 | The hand automatic sampling mode switching device of quantitative fluorescence analysis instrument |
-
2009
- 2009-05-26 CN CN2009201568115U patent/CN201413292Y/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102486447A (en) * | 2010-12-06 | 2012-06-06 | 沈阳理工大学 | Rapid detection device of milk components |
| CN106248603A (en) * | 2016-09-27 | 2016-12-21 | 济南恒辉科济食品配料有限公司 | Food is suspended and opaque system Stability Determination detection method and detection device |
| CN107966433A (en) * | 2016-10-19 | 2018-04-27 | 姬志刚 | A kind of dateplum persimmon protein rapid detection apparatus |
| CN110487764A (en) * | 2019-08-30 | 2019-11-22 | 天津陆海石油设备系统工程有限责任公司 | The hand automatic sampling mode switching device of quantitative fluorescence analysis instrument |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20100224 |
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| CX01 | Expiry of patent term |