CN2434678Y

CN2434678Y - Bacterial endotoxin analyzer

Info

Publication number: CN2434678Y
Application number: CN 00238539
Authority: CN
Inventors: 何永胜
Original assignee: Beijing Gold Mountainriver Tech Development Co ltd
Current assignee: Tianjin Yirui Biological Engineering Co., Ltd.
Priority date: 2000-06-28
Filing date: 2000-06-28
Publication date: 2001-06-13
Anticipated expiration: 2010-06-28

Abstract

The utility model relates to a bacterial endotoxin analyzer, which is composed of an outer shell, a single chip computer which is arranged on a soleplate, an integrated circuit, a temperature controller, an electric controller, relevant circuits, a reactor which is arranged on the soleplate, photoelectric detectors which are arranged on the reactor, a heater and a constant temperature block. The inner part of each set of photoelectric detector is provided with a blue light-emitting diode and a silicon blue cell, and the front end of the blue light-emitting diode is provided with a collimating hole. The utility model can simultaneously realize the quantitative analysis of bacterial endotoxin through dynamic nephelometric analysis, dynamic colorimetry and gel limit method, and can be suitable for the quantitative analysis of the bacterial endotoxin in various body fluids of medicine, food and the human body.

Description

Bacteria endotoxin detector

The utility model relates to and a kind ofly produces agglutinating reaction by tachypleus amebocyte lysate and micro-bacterial endotoxin and realize bacteria endotoxin detector to bacterial endotoxin quantitative measurement in the various body fluid of medicine, medical apparatus, water, food and drink, clinical examination and human body.

Endotoxin is the class lipopolysaccharides body in the gram-negative bacterial cell wall structure, and this kind lipopolysaccharides is transported to the constituent that cell surface constitutes the cell wall adventitia after bacterial cell wall is synthetic, discharges from eucaryotic cell structure in the dead molten routed back of bacterium.Endotoxin has multiple toxic action to the host on the one hand, and it can bring into play many biologic activity useful to body again on the other hand, and the bacterial endotoxin quantitative measurement has important directive significance for medicine production, clinical examination and Food Inspection etc.

The bacterial endotoxin quantitative measurement can be divided into gel method, turbidimetry, colourimetry, ELISA (enzyme-linked immunoassay) method, at present, kinetic turbidimetric assay and dynamically colourimetry adopted widely, and received by many state-promulgated pharmacopoeia and to be started; The frequent bacteria endotoxin detector that uses, its measuring principle are that the turbidity according to endotoxin and tachypleus amebocyte lysate reactant liquor changes (through light amount ratio) the single order logarithm value of value required time and the inversely proportional relation of logarithm value of bacterial endotoxin concentration and quantitative.

General bacteria endotoxin detector is by automatically controlled part, the temperature control part, the reactor part, photoelectric detection parts etc. constitute, a common analyzer can only carry out endotoxic quantitative measurement with a kind of kinetic turbidimetric assay, when adopting dynamic colorimetric method for determining, must carry out with another analyzer, and photoelectric detection part must adopt the center at the 405nm wavelength, and the light of narrow range, and to obtain close limit like this and like this light of wavelength, existing analyzer can only adopt optical filter or grating to obtain both at home and abroad, this structure is the cost height not only, and is difficult to obtain the light of any multichannel 405nm wavelength; Generally adopt incandescent lamp or wavelength to realize that greater than the red light emitting diodes of 700nm and dark knot normal optical battery dynamic colourimetry carries out endotoxic quantitative measurement in recent years, this structure can not be implemented in carries out kinetic turbidimetric assay simultaneously on same the analyzer, dynamically colourimetry and the gel quantitative measurement of three kinds of methods of method of limiting the quantity of, can not realize the quantitative measurement of quick bacteria endotoxin, sense cycle is long, and because the aperture of light path is bigger, its diameter and length ratio are greater than 1, the bottom surface distance in light path axis and test tube hole is bigger, generally, influence the light path examination scope greater than 5mm.

The purpose of this utility model is to provide a kind of sense cycle short, examination scope is wide, can realize quick kinetic turbidimetric assay quantitative measurement, can obtain multichannel light examination scope, can realize kinetic turbidimetric assay simultaneously, dynamic colourimetry and the gel bacteria endotoxin detector of method three therapeutic methods of traditional Chinese medicine unification quantitative measurement bacterial endotoxin of limiting the quantity of.

The purpose of this utility model is achieved in that

A kind of bacteria endotoxin detector is by shell, is arranged on single-chip microcomputer, integrated circuit, attemperating unit and electric control gear and interlock circuit thereof on the base plate; Be arranged on reactor on the base plate, be contained in that photoelectric detector, well heater and isothermal block on the reactor constitute, in every cover photoelectric detector blue LED is housed.The front end of blue LED is provided with collimating aperture.The diameter of collimating aperture and length ratio are less than 1.

The utility model has improved the structure of existing endotoxin determinator photoelectric detection part, be provided with collimating aperture and blue LED be housed and silicon blue cell, can obtain the light of any multichannel wavelength, enlarge the light examination scope, can realize kinetic turbidimetric assay simultaneously, dynamic colourimetry and the gel method three therapeutic methods of traditional Chinese medicine unification quantitative measurement bacterial endotoxin of limiting the quantity of, the utility model is easy and simple to handle, sense cycle is short, check quick and precisely, just can finish in 30～40 minutes, examination scope is wide, from 0.001～1000EU/ml, have wide range of applications, detection by quantitative can monitor the endotoxin content variation tendency in the medicine production, is convenient to producer and in time takes measures, avoid substandard product occurring and cause economic loss, simultaneously, quantitative measurement is significant to the judgement of the diagnosis of numerous disease and result of treatment, is particularly suitable for the level of endotoxin in the multiple body fluid of human body is carried out quantitative measurement.

Description of drawings

Fig. 1 is the utility model structural representation;

Fig. 2 is an A-A cut-open view among Fig. 1;

Fig. 3 be among Fig. 2 B to cut-open view;

Fig. 4 be among Fig. 2 C to cut-open view;

Fig. 5 is a light source circuit board structure partial schematic diagram of the present utility model;

Fig. 6 is a photosignal dash receiver structure partial synoptic diagram of the present utility model.

The utility model is described in further detail below in conjunction with accompanying drawing and preferred embodiment:

Consult shown in Figure 1, bacteria endotoxin detector from outside watch mainly and constituting by shell 1, reactor 2, panel 4, pilot lamp 5, test lamp 6, test tube hole 7 etc., its reactor 2 is provided with several test tube holes 7, each test tube hole 7 corresponding test lamp 6, can insert standard test tube 3 in the test tube hole 7, panel 4 is provided with power light and temperature control pilot lamp 5, and shell 1 connects with base plate.

Consult shown in Figure 2, among the figure shell 1 is taken off, bacteria endotoxin detector structure internally sees it mainly is by photosignal dash receiver 8, photoelectric detector 9, well heater and isothermal block 10, light source wiring board 12 and base plate 11 constitute, its single-chip microcomputer, integrated circuit, attemperating unit and electric control gear and interlock circuit thereof etc. (all not drawing among the figure) are prior art, be arranged on the base plate 11, some cover photoelectric detectors 9 are housed on the reactor 2, the well heater and isothermal block 10 and the temperature sensor that constitute the constant temperature function also are housed on the reactor 2, are coupled as one and connect firmly on base plate 11 by screw.

Consult Fig. 3 simultaneously, shown in Figure 4, the light path endoporus of every suit photoelectric detector 9 is equipped with the silicon blue cell 16 of the blue LED 14 of a 350-550nm wavelength and a corresponding with it shallow junction, the P-N knot junction depth of silicon blue cell 16 is less than 100 μ m, microswitch 13 also is housed in the photoelectric detector 9, light source wiring board 12 welding of the leg of the leg of microswitch 13 and blue LED 14 and photoelectric detector 9 one sides, the front end of blue LED 14 is collimating apertures 15 of light path, the diameter of collimating aperture 15 and length ratio are less than 1, collimating aperture 15 communicates with test tube hole 7, the bottom surface in axially bored line and test tube hole 7 distance is less than 5mm in the light path, photoelectric detector 9 upper ends are provided with indicator lamp hole, green LED 17 is housed, photosignal dash receiver 8 welding of its pin and silicon blue cell 16 both positive and negative polarities and photoelectric detector 9 opposite sides in the hole.

Consult shown in Figure 5, light source wiring board 12 is bound up on photoelectric detector 9 one sides, be aligned in sequence with several on the light source wiring board 12, as eight blue LEDs 14 and four resistance 19, eight groups of legs that can insert microswitch 13, light source wiring board 12 two ends are provided with line ranking 18, are connected with automatically controlled part, temperature control part and interlock circuit, single-chip microcomputer, integrated circuit etc. by flat line.

Consult shown in Figure 6, photosignal dash receiver 8 is bound up on photoelectric detector 9 opposite sides, be aligned in sequence with several on the photosignal dash receiver 8, as eight silicon blue cells 16 and eight resistance 19, photosignal dash receiver 8 two ends are provided with line ranking 18, are connected with automatically controlled part, temperature control part and interlock circuit, single-chip microcomputer, integrated circuit etc. by flat line.

Appearance and modeling of the present utility model, reaction type shape, test tube hole position number and spread pattern can arbitrarily design as required, are not subjected to the restriction of the utility model embodiment, and the utility model is intended to protect the improvement structure of photoelectric detection part.

Principle of work of the present utility model: the measuring principle of bacteria endotoxin detector is to change (seeing through light amount ratio) the single order logarithm value of value required time and the inversely proportional relation of logarithm value of bacterial endotoxin concentration and quantitative according to the turbidity of endotoxin and tachypleus amebocyte lysate reactant liquor.The utility model carries out the communication of data acquisition, temperature control and host computer by single-chip microcomputer, and host computer carries out computing to data, and the concrete course of work is as follows:

1, connects bacteria endotoxin detector and computing machine with Serial Port Line, open the power switch 5 of analyzer, open computer power switch;

2, computing machine enters the WIN95/WIN98 system, and the operation bacterial endotoxin is measured software;

3, press collection button in the software program, enter acquisition state;

4, after the power switch of analyzer was opened 30 minutes, the closed circuit control circuit that well heater and isothermal block 10 and temperature sensor form accurately was controlled at the isothermal block of analyzer in the scope of positive and negative 1 degree of 37 degree under the manipulation of single-chip microcomputer;

5, the sample tube 3 that will mix tachypleus amebocyte lysate inserts in the test tube hole 7, and this moment, microswitch 13 sent signal, and single-chip microcomputer carries out timing and image data respectively successively to the hole of inserting test tube;

6, blue LED 14 sends Ray Of Light, passes the mixed liquor in the test tube, beats on photoelectric cell 16, and light signal changes into electric signal, changes into digital signal by single-chip microcomputer and sends to host computer;

7, the single-chip microcomputer in the analyzer sends handshake to host computer, in 10 seconds of every interval, just the data of being gathered is sent to host computer;

8, host computer calculates the data of receiving, draws the S curve in each test tube hole;

9, computing machine is reported to the police to 60 minutes test tube hole 7 of reaction time arrival, test tube 3 can be extracted flip vertical 180 degree and carry out the gel observation, and contrast with quantitative result; After all test tubes 3 arrived 60 minutes, computing machine and analyzer finished to gather automatically, can be by computing machine with the quantitative result output that calculates;

10, shutdown finishes.

Claims

1, a kind of bacteria endotoxin detector is by shell (1), is arranged on single-chip microcomputer, integrated circuit, attemperating unit and electric control gear and interlock circuit thereof on the base plate (11); Be arranged on reactor (2) on the base plate (11), be contained in that photoelectric detector (9), well heater and isothermal block (10) on the reactor (2) constitute, it is characterized in that being equipped with in every cover photoelectric detector (9) blue LED (14).

2, bacteria endotoxin detector according to claim 1 is characterized in that the front end of blue LED (14) is provided with collimating aperture (15).

3. bacteria endotoxin detector according to claim 1 and 2 is characterized in that the diameter of collimating aperture (15) and length ratio are less than 1.