CN208888137U - A kind of chloramphenicol fluorescence immunoassay quantifies poct detection kit - Google Patents
A kind of chloramphenicol fluorescence immunoassay quantifies poct detection kit Download PDFInfo
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- CN208888137U CN208888137U CN201820923176.8U CN201820923176U CN208888137U CN 208888137 U CN208888137 U CN 208888137U CN 201820923176 U CN201820923176 U CN 201820923176U CN 208888137 U CN208888137 U CN 208888137U
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- chloramphenicol
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- card slot
- test tube
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Abstract
The utility model discloses a kind of chloramphenicol fluorescence immunoassays to quantify poct detection kit comprising box body and box cover；Box body inside bottom is filled with heelpiece, and heelpiece is equipped with card slot, is fitted with reagent strip, detection shell and test tube in card slot；Detection shell includes upper housing and lower case, the side of upper housing and the side of lower case are hinged, the other side is fixed by buckle and the other side of lower case, the accommodation groove for storing reagent item is equipped in lower case, it accommodates trench bottom and is equipped with anti-skidding heelpiece, well B and peep hole B are offered on upper housing, the extruding item to match with accommodation groove is equipped on the inside of upper housing, and one end of detection shell, which is equipped with, hooks drawing portion.The detection shell of kit self-contained reagent item and fixating reagent item provided by the utility model, the problem of detection can mutually be linked with shell with the drag hook that have inside immunity analysis instrument or other detecting instruments, can be avoided the position inaccurate that leads to reagent strip because of manual operation mistake itself.
Description
Technical field
The utility model relates to technical field of biological more particularly to a kind of chloramphenicol fluorescence immunoassay to quantify poct detection
Kit.
Background technique
Chloramphenicol is white or micro- yellowish leukorrhea green acicular crystal, is slightly soluble in water, it is organic to be soluble in methanol, ethyl alcohol, propyl alcohol etc.
Solvent, chloramphenicol are a kind of spectrum antibiotic, are risen to the treatment of all kinds of poultry, domestic animal, aquatic products and various communicable diseases
Important function, because its good anti-bacterial effect is cheap is widely used in animal husbandry, but it is to mankind's toxic side effect
It is larger, it is endangered if remaining in and can be constituted to human health in animal food, currently, China has forbidden producing in animal food
Chloramphenicol is used in the process.
It is mainly at present instrument analytical method to the detection mode of chloramphenicol, needs a large amount of instrument and equipment, and these set
It is standby expensive, it is complicated for operation, the time is long, and can not be used at the scene with basic small-size laboratory, coherent detection reagent strip
There is also problems, for example, reagent strip smoothly constant speed must be translated through pinpoint scanning detection apparatus, at present simultaneously
It is not carried out pinpoint device, in the detection process, it is most likely that lead to reagent strip because of manual operation mistake itself
Position inaccurate in turn results in analyzer Scanning Detction display window and deviation occurs, seriously affects test speed and accuracy.
Summary of the invention
In order to solve the above-mentioned technical problem, the purpose of the utility model is to provide a kind of chloramphenicol fluorescence immunoassays to quantify poct
Detection kit, the kit carry detection shell, the drawing that can have with immunity analysis instrument or other detecting instruments inside
The problem of hook mutually links, and can be avoided the position inaccurate that leads to reagent strip because of manual operation mistake itself.
The utility model is achieved through the following technical solutions: a kind of chloramphenicol fluorescence immunoassay quantifies poct detection reagent
Box, including box body and the box cover being connect with box body；Box body inside bottom is filled with heelpiece, and heelpiece is equipped with card slot, in card slot
It is fitted with reagent strip, detection shell and test tube；Detection shell includes upper housing and lower case, the side of upper housing and lower casing
The side of body is hinged, and the other side is fixed by buckle and the other side of lower case, is equipped in lower case for storing reagent item
Accommodation groove, accommodating trench bottom are equipped with anti-skidding heelpiece, offer on upper housing and match with the well A and peep hole A of reagent strip
Well B and peep hole B, the extruding item to match with accommodation groove is equipped on the inside of upper housing, one end of detection shell is equipped with
Hook drawing portion；The chloramphenicol antibody and time resolution europium of time resolution europium fluorescent microsphere label are coated on the bonding pad of reagent strip
The rabbit igg antibody of fluorescent microsphere label, is coated with chloramphenicol-BSA antigen, the Quality Control C line of reagent strip at the detection T line of reagent strip
Place is coated with goat-anti rabbit secondary antibody.
At least following beneficial effect is the utility model compared with prior art: kit provided by the utility model is certainly
Detection shell with reagent strip and fixating reagent item, the high degree of specificity and unique fluorescence that reagent strip passes through immune response
Microballoon is combined as antibody trace labelling object with highly sensitive time-resolved fluoroimmunoassay chromatographic technique and POCT theory,
Realize the purpose of quantitative detection；Detection can mutually be hooked with shell with the drag hook having inside immunity analysis instrument or other detecting instruments
Even, so that being suitable for self-loading reagent strip disengaging detecting instrument immunity analysis instrument or other detecting instruments, thus, it is possible to keep away
Exempt to lead to reagent strip because of manual operation mistake itself position inaccurate, in turn results in the sight of immunity analysis instrument scanning detecting result
The problem of occurring deviation when examining and seriously affecting measuring accuracy.
Further, card slot inner wall and bottom are coated with waterproof material, and waterproof material can be used aluminium-foil paper, can effectively prevent
Only reagent strip is influenced to cause the phenomenon of test result inaccuracy by outside moisture.
Further, it is also provided with ID card slot on heelpiece, ID card is inserted in ID card slot, is stored with reagent mark in the ID card
Directrix curve information.ID card is chip card, its internal information can be read directly in POCT analyzer, improves the detection effect of concentration
Rate.
Further, hooking drawing portion is v-notch.
Further, reagent strip includes plastic shell, the carrier board being mounted on inside plastic shell and successively pastes
One end of the sample pad of carrier board upper surface, bonding pad, nitrocellulose membrane, water absorption pad, sample pad is overlapped in one end of bonding pad
On, the other end of bonding pad is overlapped on one end of nitrocellulose membrane, and one end of water absorption pad is overlapped in the another of nitrocellulose membrane
On end, cellulose nitrate film surface is successively arranged detection T line, Quality Control C line along liquid chromatography(LC) direction, and plastic shell is equipped with and sample
Pad corresponding well A and peep hole A corresponding with detection T line and Quality Control C line.
Further, the test tube includes sample extracting solution test tube and sample diluting liquid test tube, in sample extracting solution test tube
The liquid of storage is methanol solution, the liquid stored in sample diluting liquid test tube be containing 0.5% (m/v) bovine serum albumin(BSA),
The phosphate buffer solution of the 0.01 mol/L pH7.4 of 2.5% (m/v) Tween20.
Further, at least half of reagent strip is inserted into card slot, is equipped at the top of reagent strip and is held slot, hold slot position in
Outside card slot；When reagent strip arrangement is more intensive, operator can hold holding slot and easily extract reagent strip.
Further, reagent strip is equipped with label paper and bar code, and label paper can be used for experimenter and record sample information,
Bar code can be used for the reagent strip that time-resolved fluoroimmunoassay quantifies POCT analyzer identification different batches.
Further, constant temperature panel is equipped in card slot, the constant temperature panel is connect with condenser plate, condenser plate and control switch
Connection, constant temperature panel is for keeping reagent strip constant temperature.
Further, cystosepiment is filled with inside box cover, offered on cystosepiment with reagent strip, detection shell, test tube,
The groove that ID card matches, after box cover is fastened on box body, the cystosepiment of box cover can further fix reagent strip, test tube, ID
Card, prevents it from shaking in transportational process and causes test error.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model；
Fig. 2 is the structural schematic diagram of heelpiece；
Fig. 3 is the structural schematic diagram of detection shell；
Fig. 4 is the schematic diagram of internal structure of reagent strip；
Fig. 5 is the external structure schematic diagram of reagent strip.
In figure, 1, box body；2, box cover；3, heelpiece；31, card slot；4, test tube；5, shell is used in detection；51, upper housing；511,
Buckle；512, well B；513, peep hole B；514, item is squeezed；52, lower case；521, accommodation groove；522, anti-skidding heelpiece；53,
Hook drawing portion；6, reagent strip；60, plastic shell；61, carrier board；62, sample pad；63, bonding pad；64, nitrocellulose membrane；65, it inhales
Water cushion；66, T line is detected；67, Quality Control C line；68, well A；69, peep hole A；6-1, slot is held；6-2, label paper；6-3, item
Shape code；7, ID card slot；8, ID card；9, cystosepiment；10, groove.
Specific embodiment
The utility model is described further with reference to the accompanying drawing.
Chloramphenicol fluorescence immunoassay as shown in Figs. 1-5 quantifies poct detection kit, including box body 1 and connects with box body 1
The box cover 2 connect；Inside bottom is filled with heelpiece 3, and heelpiece is equipped with card slot 31, is fitted with test tube 4, detection shell in card slot 31
5 and reagent strip 6；Detection shell 5 includes upper housing 51 and lower case 52, and the side of upper housing 51 and the side of lower case 52 are cut with scissors
It connects, the other side is fixed by buckle 511 and the other side of lower case 52, and the appearance for storing reagent item 6 is equipped in lower case 52
Slot 521 is set, 521 bottom of accommodation groove is equipped with anti-skidding heelpiece 522, and anti-skidding heelpiece 522 can prevent reagent strip from sliding, on upper housing 51
Offer the well B512 and peep hole B513 to match with the well A68 and peep hole A69 of reagent strip 4, upper housing 51
Inside is equipped with the extruding item 514 that matches with accommodation groove 521, and after upper housing 51 and lower case 52 fasten, squeezing item 514 can be with
It squeezes reagent strip 6 to compress reagent strip, when preventing transfer detection shell 5, reagent strip 6 shakes；The one of shell 5 is used in detection
End, which is equipped with, hooks drawing portion 53, and the shape for hooking drawing portion 53 can choose as v-notch, and detection shell 5 can be with by hooking drawing portion 53
The drag hook having inside immunity analysis instrument or other detecting instruments mutually links, so that being suitable for the disengaging inspection of self-loading reagent strip
Instrument immunity analysis instrument or other detecting instruments are surveyed, thus, it is possible to avoid leading to reagent strip because of manual operation mistake itself calmly
There is deviation and seriously affects asking for measuring accuracy when in turn resulting in the observation of immunity analysis instrument scanning detecting result in position inaccuracy
Topic.
Preferably, 31 inner wall of card slot and bottom are coated with waterproof material, and waterproof material can use aluminium-foil paper, waterproof material
Setting can prevent reagent strip because moisture influence and cause test inaccuracy problem.
Preferably, as shown in Figure 1, being also provided with ID card slot 7 on heelpiece 3, ID card 8 is inserted in ID card slot, in the ID card 8
It is stored with reagent standard curve information, in use, ID card 8 can be inserted directly into immunity analysis instrument or the phase of other detecting instruments
Off position is directly read automatically by it.
Specifically, as illustrated in figures 4-5, reagent strip 6 includes plastic shell 60, the carrier board being mounted on inside plastic shell 60
61 and successively paste the sample pad 62 in 61 upper surface of carrier board, bonding pad 63, nitrocellulose membrane 64, water absorption pad 65, sample
One end of pad 62 is overlapped on one end of bonding pad 63, and the other end of bonding pad 63 is overlapped on one end of nitrocellulose membrane 64,
One end of water absorption pad 65 is overlapped on the other end of nitrocellulose membrane 64,64 surface of nitrocellulose membrane along liquid chromatography(LC) direction successively
Equipped with detection T line 66, Quality Control C line 67, plastic shell 60 be equipped with well A68 corresponding with sample pad 62 and with detection
T line and the corresponding peep hole A69 of Quality Control C line.Time resolution europium (Eu) fluorescent microsphere label is coated on the bonding pad 63
Chloramphenicol antibody and time resolution europium fluorescent microsphere label rabbit igg antibody, be coated with chloramphenicol-at the detection T line
BSA antigen is coated with goat-anti rabbit secondary antibody at the Quality Control C line.When sample to be tested is added drop-wise to well by well B512
When in A68, the chloramphenicol antibody knot merga pass capillary of fluorescent microsphere label in the chloramphenicol antigen and bonding pad 63 in sample
Effect chromatographs forward, and after reaching detection zone, the fixed chloramphenicol of the detection T line 66 fluorescent microsphere label chlorine unbonded with residue is mould
Plain antibody combines, detect the concentration of chloramphenicol in the chloramphenicol antibody concentration and sample of the fluorescent microsphere label combined on T line at
Inverse ratio, the fluorescent marker concentration combined on Quality Control C line is unrelated with the concentration of chloramphenicol in sample, can be with by peep hole B513
The variation of observation detection T line 66 and Quality Control C line 67；After chromatography, POCT analyzer is quantified using time-resolved fluoroimmunoassay
It reads the fluorescence intensity of detection T line and the relative intensity of fluorescence of detection C line and calculates T/C value, pass through the standard stored in ID card
Calibration curve information calculates chloramphenicol in sample with the analysis software built in time-resolved fluorescence immune quantitative POCT analyzer automatically
Concentration.
The test tube 4 includes sample extracting solution test tube and sample diluting liquid test tube, the liquid stored in sample extracting solution test tube
Body is methanol solution, and the liquid stored in sample diluting liquid test tube is containing 0.5% (m/v) bovine serum albumin(BSA), 2.5% (m/
V) the phosphate buffer solution of the 0.01 mol/L pH7.4 of Tween20.
Based on any of the above embodiments, it is preferable that at least half of reagent strip 6 is inserted into card slot 31, such as Fig. 5
It is shown, it is equipped at the top of reagent strip 6 and holds slot 6-1, held slot 6-1 and be located at outside card slot 31, the well A68 of reagent strip 6 and sight
It examines hole A69 to be respectively positioned in card slot 31, can guarantee the drying property of reagent strip 6 in this way, improve Detection accuracy；Reagent strip 6 arranges
When more intensive, operator can hold holding slot 6-1 and easily extract reagent strip 6.
Based on any of the above embodiments, it is preferable that as shown in figure 5, reagent strip 6 is equipped with label paper 6-2 and item
Shape code 6-3, label paper can be used for experimenter and record sample information, and bar code can be used for time-resolved fluoroimmunoassay and quantify POCT
The reagent strip of analyzer identification different batches.
Based on any of the above embodiments, it is preferable that constant temperature panel is equipped in card slot, for keeping reagent strip permanent
Temperature, the constant temperature panel are connect with condenser plate, and condenser plate is connect with control switch.
Based on any of the above embodiments, it is preferable that as shown in Figure 1, being filled with cystosepiment 9, foam inside box cover 2
The groove 10 to match with test tube 4, detection shell 5, reagent strip 6,8 top of ID card is offered on plate 9, thus by test tube 4, inspection
It surveys with shell 5, reagent strip 6, fixed intact, the damage caused by avoiding in transportational process of ID card 8.
When being detected using the utility model to the chloromycetin content in feed addictive, the method is as follows:
The pre-treatment of 1.1 samples
1.1.1 the processing of egg, animal muscle tissue, Feed Sample: egg being opened and is homogenized, ground 40 mesh of feed
Sieve, animal muscle tissue takes edible meat about 200g, with homogenizer it is thoroughly homogeneous after, it is even to accurately weigh 2.0 ± 0.02g sample
After matter in 15ml centrifuge tube, 10ml sample extracting solution is added, with vortex oscillator mechanical shaking extraction 5min；After extraction,
4000rpm is centrifuged 2min, takes 100 μ l supernatants, 200 μ l sample diluting liquids is added to, to sample detection after mixing.
1.1.2 the processing of the fluid samples such as milk, honey: 100 μ l fluid samples is directly taken to be added to the dilution of 200 μ l samples
In liquid, sample detection after mixing.
1.2 detection process and result
1.2.1 constant-temperature incubation device is switched on, is arranged 37 DEG C of temperature, rising to 37 DEG C of constant rears to temperature can be used；
1.2.2 after fluorescence immunoassay quantifies POCT analyzer preheating 5min, it is inserted into ID card corresponding to the utility model, and
Scan bar code corresponding to this batch products；
1.2.3 test strips are taken out from card slot, reagent strip is mounted in detection shell, are horizontally placed at constant-temperature incubation device
On；
1.2.4 sample to be tested of the 100 μ l Jing Guo pre-treatment is drawn with pipettor to be added in the well of test strips, cover and incubate
Device lid is educated, starts timing and is incubated for 5min；
1.2.5 the detection with test strips is inserted into time-resolved fluoroimmunoassay with shell after 5min and quantifies POCT analysis
In instrument test strips slot, fixed by hooking drawing portion, touch screen point reading, reading value be in sample chloramphenicol contain
Amount；
1.2.6 it if testing result exceeds the upper limit of the range of linearity, is detected again after being diluted with sample diluting liquid, detection knot
Fruit is multiplied by extension rate, the as ultimate density of sample.
The utility model by the high degree of specificity and unique fluorescent microsphere of immune response as antibody trace labelling object,
And combined with highly sensitive time-resolved fluoroimmunoassay chromatographic technique and POCT theory, utilize the original of competitive immunoreaction
Reason, only with unification platform time resolution immune quantitative POCT analyzer, can be realized it is easy quickly, the detection egg of accurate quantitative analysis,
The content of chloramphenicol in the samples such as honey, milk, animal muscle tissue, feed；Fluorescence mark in the reagent strip of the utility model
Note object is to wrap up the fluorescent microsphere being process through special using lanthanide series Eu, and fluorescence intensity ratio general labeling object wants high by 100
Times or more, according to its luminous characteristics and spectral characteristic, transmitting fluorescence is measured with TIME RESOLVED TECHNIQUE, passes through time delay and wavelength point
It distinguishes, effectively excludes the interference of non-specific fluorescence, greatly improve the sensitivity for analysis of the utility model；The utility model
High sensitivity, high specificity, the range of linearity are wide, simple and quick, meet the requirement of National Animal derived food safety detection.
Sensitivity with the kit of the utility model detection chloramphenicol is 0.05 μ g/kg, and the range of linearity is 0.2-10 μ g/
Kg, accuracy TIANZHU XINGNAO Capsul be 80-125%, under 10 μ g/kg concentration levels with other hormone medicine no cross reactions, sample
This solution can determine the content of chloramphenicol in 5 minutes accurate quantitative analysis, and testing result meets National Animal derived food safety
Testing requirements.
Embodiment described above only expresses specific embodiment of the present utility model, and description is more specific and detailed
Carefully, but it should not be understood as limiting the scope of the patent of the utility model.It should be pointed out that for the general of this field
For logical technical staff, without departing from the concept of the premise utility, various modifications and improvements can be made, these are all
Belong to the protection scope of the utility model.
Claims (8)
1. a kind of chloramphenicol fluorescence immunoassay quantifies poct detection kit, which is characterized in that connect including box body (1) and with box body
The box cover (2) connect；Box body inside bottom is filled with heelpiece (3), and heelpiece is equipped with card slot (31), be fitted in card slot test tube (4),
Detection shell (5) and reagent strip (6)；Detection shell includes upper housing (51) and lower case (52), the side of upper housing with
The side of lower case is hinged, and the other side is fixed by buckle (511) and the other side of lower case, is equipped in lower case for storing
The accommodation groove (521) of reagent strip, accommodating trench bottom are equipped with anti-skidding heelpiece (522), the sample-adding with reagent strip are offered on upper housing
Hole A(68) and peep hole A(69) the well B(512 that matches) and peep hole B(513), it is equipped on the inside of upper housing and accommodation groove
The extruding item (514) to match, one end of detection shell, which is equipped with, hooks drawing portion (53), and hook drawing portion is v-notch；The knot of reagent strip
Close the chloramphenicol antibody that time resolution europium fluorescent microsphere label is coated on pad (63) and time resolution europium fluorescent microsphere label
Rabbit igg antibody, chloramphenicol-BSA antigen is coated at the detection T line (66) of reagent strip, is wrapped at the Quality Control C line (67) of reagent strip
There is goat-anti rabbit secondary antibody；At least half of reagent strip is inserted into card slot, is equipped at the top of reagent strip and is held slot (6-1), and slot is held
Outside card slot, the well A and peep hole A of reagent strip are respectively positioned in card slot.
2. chloramphenicol fluorescence immunoassay according to claim 1 quantifies poct detection kit, which is characterized in that card slot inner wall
And bottom is covered with waterproof material.
3. chloramphenicol fluorescence immunoassay according to claim 1 quantifies poct detection kit, which is characterized in that on heelpiece also
It offers ID card slot (7), inserts ID card (8) in ID card slot, be stored with reagent standard curve information in the ID card.
4. chloramphenicol fluorescence immunoassay according to claim 1 quantifies poct detection kit, which is characterized in that reagent strip packet
It includes plastic shell (60), the carrier board (61) that is mounted on inside plastic shell and successively paste sample in carrier board upper surface
(62), bonding pad (63), nitrocellulose membrane (64), water absorption pad (65) are padded, one end of sample pad is overlapped on one end of bonding pad,
The other end of bonding pad is overlapped on one end of nitrocellulose membrane, and one end of water absorption pad is overlapped in the other end of nitrocellulose membrane
On, cellulose nitrate film surface is successively arranged detection T line (66), Quality Control C line (67) along liquid chromatography(LC) direction, and plastic shell is equipped with
Well A(68 corresponding with sample pad) and peep hole A(69 corresponding with detection T line and Quality Control C line).
5. chloramphenicol fluorescence immunoassay according to claim 1 quantifies poct detection kit, which is characterized in that the test tube
Including sample extracting solution test tube and sample diluting liquid test tube, the liquid stored in sample extracting solution test tube is methanol solution, sample
The liquid stored in dilution test tube be containing 0.5% (m/v) bovine serum albumin(BSA), 2.5% (m/v) Tween20 0.01
The phosphate buffer solution of mol/L pH7.4.
6. chloramphenicol fluorescence immunoassay according to claim 1 quantifies poct detection kit, which is characterized in that on reagent strip
Equipped with label paper (6-2) and bar code (6-3).
7. chloramphenicol fluorescence immunoassay according to claim 1 quantifies poct detection kit, which is characterized in that test tube bottom
Card slot in be equipped with constant temperature panel, the constant temperature panel connect with condenser plate, and condenser plate is connect with control switch.
8. chloramphenicol fluorescence immunoassay according to claim 1 quantifies poct detection kit, which is characterized in that inside box cover
Filled with cystosepiment (9), the groove (10) to match with reagent strip, detection shell, test tube is offered on cystosepiment.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820923176.8U CN208888137U (en) | 2018-06-14 | 2018-06-14 | A kind of chloramphenicol fluorescence immunoassay quantifies poct detection kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820923176.8U CN208888137U (en) | 2018-06-14 | 2018-06-14 | A kind of chloramphenicol fluorescence immunoassay quantifies poct detection kit |
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| CN208888137U true CN208888137U (en) | 2019-05-21 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112881356A (en) * | 2021-01-18 | 2021-06-01 | 上海雄图生物科技有限公司 | High-flux fluorescence immunoassay quantitative POCT analysis device |
-
2018
- 2018-06-14 CN CN201820923176.8U patent/CN208888137U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112881356A (en) * | 2021-01-18 | 2021-06-01 | 上海雄图生物科技有限公司 | High-flux fluorescence immunoassay quantitative POCT analysis device |
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