Medicine sensitive detecting plate
Technical field
The utility model relates to a kind of medicine sensitive detecting plate.
Background technology
Antimicrobial has played important effect to the control of bacterial infectious disease, but because the not science of medication process, blindly abuse antimicrobial, thereby cause a lot of pathogenic bacterias to produce drug resistance, make antimicrobial worse and worse to the control effect of bacteriosis, not only cause drug waste, but also delay treatment, present antibiotic application is very extensive, various pathogens are to the susceptibility difference of antibiotic medicine, the different strains of bacterium of the same race is also variant to the susceptibility of same medicine, and bacterial detection can be screened active drug to the susceptibility of antibacterials, be used for clinical treatment, to controlling the popular most important of bacterial infectious disease.Representative is detects the susceptibility of mycoplasma, now separate 7 mycoplasma species from human urogenital tract, wherein separation rate is higher and closely-related with the urogenital tract disease is Ureaplasma urealyticum (Ureaplasma urealyticum, Uu), next is mycoplasma hominis (Mycoplasmahominis, Mh), UU and MH can cause urogenital infections, very easily cause a series of pathologies, therefore diagnosis promptly and accurately and effective treatment are the keys that prevents that mycoplasma from propagating, the workflow that present stage adopts is to judge the existence of mycoplasma, the separation and Culture of mycoplasma, identify, counting, drug sensitive test etc., the medicine sensitive detecting plate that existing market provides is only made for a certain program wherein, such as making for diagnosis or for antibiotic susceptibility test, limited the property rapidly and efficiently of whole process thus to a great extent, influence is to effective control of transmission with to the good treatment of disease.
The utility model content
Technical problem to be solved in the utility model is to overcome the deficiencies in the prior art, provide a kind of efficiently, fast, medicine sensitive detecting plate accurately.
The technical scheme that the utility model adopted is: the utility model comprises detection plate body, lid, described lid and described detection plate body closely cooperate, described detection plate body is provided with and detects the hole, described detection is coated with encrusting substance in the hole, described detection hole can be divided into plurality of rows as required, and every row can be provided with several described detection holes on request; The end of described detection plate body also can be provided with one or more medicine bottle grooves as required, is used to place just using or standby medicine bottle and other bottles Guan, and described detection hole is other to be provided with the sign symbol, is used to indicate the classification that respectively detects the hole, handled easily, minimizing error.
Described detection hole comprises that control wells, culture hole, Uu identify that hole, Mh identify that hole, Uu counting hole, Mh counting hole, susceptibility detect the hole, and described control wells inoculation basal liquid is used to carry out blank in cultivation; Whether the basal liquid of sample is added in the inoculation of described culture hole, observe its color and change, and then whether judge this sample infected, as the basal liquid that is added with sample becomes redness in cultivation, i.e. expression is infected; Described Uu identifies that the hole endoperidium has Uu detection thing, and whether the basal liquid that adds sample when inoculation is observed its color and changed in described Uu identifies the hole, if become redness, represents that promptly this sample infection has Uu; Described Mh identifies that the hole endoperidium has Mh detection thing, and whether the basal liquid that adds sample when inoculation is observed its color and changed in described Mh identifies the hole, if become redness, represents that promptly this sample infection has Mh; Described Uu counting hole and described Mh counting hole are used to detect the concentration that infects mycoplasma, thereby carry out the classification of infection conditions, and the situation according to clinical appearance is divided into four classes, the first kind at present: feminine gender, promptly represent uninfection; Second class: the weak positive, mycoplasma concentration is less than 10
4Ccu/ml; The 3rd class: the positive, mycoplasma concentration is more than or equal to 10
4Ccu/ml and less than 10
6Ccu/ml; The 4th class: strong positive, mycoplasma is more than or equal to 10
6Ccu/ml; In view of the above, at described Uu counting hole endoperidium the Uu count detection thing that particular detection concentration interval is set is arranged, for example setting Uu detectable concentration interval is 10
4Ccu/ml to 10
6Ccu/ml, inoculation is added the basal liquid of sample in described Uu counting hole, observes the change color of basal liquid, and in the detectable concentration interval that sets, then basal liquid becomes redness as if the Uu concentration that infects; In like manner, described Mh counting hole endoperidium has the Mh count detection thing that particular detection concentration interval is set, and is 10 as setting Mh detectable concentration interval
4Ccu/ml to 10
6Ccu/ml, inoculation is added the basal liquid of sample in described Mh counting hole, observes the change color of basal liquid, and in the detectable concentration interval that sets, then basal liquid becomes redness as if the Mh concentration that infects; All the other detect the hole is that described susceptibility detects the hole, the number that described susceptibility detects the hole is provided with as required, and can be divided into many rows, every row's described susceptibility detects the different microbiotic of hole endoperidium, two rows or arrange susceptibility more and detect the hole bag by the microbiotic of the same race of variable concentrations, the basal liquid of inoculation interpolation sample is cultivated in 37 ℃ of environment, sees its look and becomes to record results such as sensitivity, resistance, intermediary, and measured result is significant to clinical treatment and medication; Experience and common antibiotics kind according to susceptibility detects can the optimization of described detection hole be set to two rows, and 13 of every rows detect the hole, ten kinds of different common antibiotics are carried out susceptibility simultaneously detect.
The beneficial effects of the utility model are: the utility model is provided with the plurality of detection hole, can cultivate, separate, count pathogen simultaneously, and simultaneously multiple microbiotic be carried out susceptibility and detect, and makes detections, selects medicine, treatment is faster efficiently; The utility model is provided with one or more medicine bottle grooves in the end of detecting plate body, is used to place and is just using or standby medicine bottle and other bottles Guan, brings convenience to operating process; Detection of the present utility model hole is other to be provided with the sign symbol, as Uu, Mh, all kinds of microbiotic code names etc., indicates the classification that respectively detects the hole thus, makes things convenient for the staff to operate, and prevents to produce error because of obscuring.
Description of drawings
Fig. 1 is the structural representation that the utility model detects plate body;
Fig. 2 is the structural representation of the utility model lid;
Fig. 3 is the A-A cut-open view of the utility model Fig. 1.
Embodiment
As Fig. 1, Fig. 2, shown in Figure 3, the utility model comprises detection plate body 1, lid 2, described lid 2 closely cooperates with described detection plate body 1, described detection plate body 1 is provided with and detects hole 3, be coated with encrusting substance in the described detection hole 3, described detection hole 3 can be divided into plurality of rows as required, and every row can be provided with several described detection holes 3 on request, in the present embodiment, described detection hole 3 is set to two rows, 13 of every rows detect the hole, comprising control wells 31, culture hole 32, Uu identifies hole 33, Mh identifies hole 34, Uu counting hole 35, Mh counting hole 36, all the other detect hole 37 for susceptibility; The end of described detection plate body 1 also can be provided with one or more medicine bottle grooves 4 as required, present embodiment is provided with a medicine bottle groove 4, be used to place and just using or standby medicine bottle and other bottles Guan, described detection hole 3 other being provided with indicate symbol 5, are used to indicate the classification that respectively detects hole 3.
During operation, described control wells 31 inoculation basal liquids are used to carry out blank in cultivation; Whether the basal liquid of samples is added in 32 inoculations of described culture hole, and 37 ℃ of environment are cultivated, observe its color and change, and then whether judge this sample infected, as the basal liquid that is added with sample becomes redness in cultivation, i.e. expression is infected; Whether described Uu identifies hole 33 endoperidium Uu detection thing, and inoculation is added the basal liquid of sample in described Uu identifies hole 33, observe its color and change, if become redness, represents that promptly this sample infection has Uu; Whether described Mh identifies hole 34 endoperidium Mh detection thing, and inoculation is added the basal liquid of sample in described Mh identifies hole 34, observe its color and change, if become redness, represents that promptly this sample infection has Mh; Described Uu counting hole 35 and described Mh counting hole 36 are used to detect the concentration that infects mycoplasma, at described Uu counting hole 35 endoperidiums the Uu count detection thing that particular detection concentration interval is set are arranged, and Uu detectable concentration interval is set at 10 in the present embodiment
4Ccu/ml to 10
6Ccu/ml, inoculation is added the basal liquid of sample in described Uu counting hole 35, observes the change color of basal liquid, and in the detectable concentration interval that sets, then basal liquid becomes redness as if the Uu concentration that infects; In like manner, particular detection concentration is set is 10 to described Mh counting hole 36 endoperidiums
4Ccu/ml to 10
6The Mh count detection thing of ccu/ml, inoculation add the basal liquid of sample in described Mh counting hole 36, observe the change color of basal liquid, and in the detectable concentration interval that sets, then basal liquid becomes redness as if the Mh concentration that infects; All the other detect the hole is that described susceptibility detects hole 37, detect ten kinds of different microbiotic of hole 37 endoperidiums in described susceptibility, the corresponding susceptibility of two rows detects hole 37 endoperidiums microbiotic of the same race, the antibiotic concentration height of last package quilt, the antibiotic concentration of following package quilt is low, between two row's susceptibility detection holes 37, indicate corresponding antibiotic symbol for differentiation, in the present embodiment, ten kinds of microbiotic are respectively: DOX, MIN, CPF, OFL, SPA, ROX, AZI, JOS, SPE, the basal liquid that sample is added in inoculation detects in the hole 37 in above-mentioned susceptibility, 37 ℃ of environment are cultivated down, observing its look becomes to record sensitivity, resistance, results such as intermediary, measured result is significant to clinical treatment and medication.