CN201254567Y - Microorganism fermentation transplantation device - Google Patents

Microorganism fermentation transplantation device Download PDF

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Publication number
CN201254567Y
CN201254567Y CN 200820068509 CN200820068509U CN201254567Y CN 201254567 Y CN201254567 Y CN 201254567Y CN 200820068509 CN200820068509 CN 200820068509 CN 200820068509 U CN200820068509 U CN 200820068509U CN 201254567 Y CN201254567 Y CN 201254567Y
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China
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fermentation
tanks
tank
liquid material
material conveying
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Expired - Fee Related
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CN 200820068509
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Chinese (zh)
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何之平
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The utility model relates to a microbe fermentation and culture transferring device which belongs to microbe fermentation equipment. The device comprises seed tanks, fermentation tanks, a sterile air supply system, liquid material conveying pipes, a valve and an electric stirring machine. The seed tanks are mutually communicated with each other by the conveying pipes, the seed tanks are communicated with the fermentation tanks by the conveying pipes, the fermentation tanks are mutually communicated with each other by the conveying pipes; the characteristics are that: liquid material conveying pumps are partially or completely connected in series in the liquid material conveying pipes between the seed tanks, between the seed tank and the fermentation tank and between the fermentation tanks, thus leading the seed tanks and the fermentation tanks to always keep the sterile air system under moderate pressure and continuously supply sterile air to the seed tanks or the preceding-stage fermentation tanks, thus realizing the transfer of the strains which are growing vigorously and the fermentation products under the circumstance of enough oxygen supply, avoiding losses caused by the weakness and death of the strains due to lacking oxygen during the culture transferring process. By utilizing the microbe fermentation and culture transferring device, the fermentation period is shortened, the fermentation efficiency is remarkably increased and the production cost is reduced.

Description

A kind of microbial fermentation culture transferring device
Technical field
A kind of microbial fermentation culture transferring of the utility model device, the microorganism belonging to genus fermentation equipment
Background technology
Existing microbial fermentation culture transferring device, as shown in Figure 3, comprise seeding tank, fermentor tank, sterile air plenum system, liquid material conveying tube, valve and electric blender, microbial strains is in seeding tank, keep under the continuous technological condition for fermentation of supplying with and stipulating of sterile air, after growth and maturity are some amount mycelia, bacterium amount, need the kind liquid of growth and maturity in the seeding tank is delivered to fermentor tank by pipe connecting by processing requirement, realize that mature strains makes its further fermentation in fermentor tank to the transfer of fermentor tank in the seeding tank.This transfer process is called " culture transferring " in the industry, when implementing culture transferring, at first will close the sterile air outlet valve, makes unimpeded sterile air path blocked, meanwhile, promotes the seeding tank internal pressure, and tensimeter shows that its pressure is 1-2Kg/cm 2Under this supercharging condition, in approximately 30-60 minute culture transferring process, it is few even be zero to plant dissolved oxygen in the liquid in the seeding tank, originally the microorganism of normal vigorous growth is in the severe depletion of oxygen state in seeding tank, causes growth impaired, even causes partly bacterial classification death, cause its early growth to the fermentor tank of microbial strains culture transferring slow, whole fermentative production cycle stretch-out and production efficiency are low.In order to accelerate culture transferring speed, general measure is to promote the seeding tank internal pressure, but in order to ensure operational safety, the lifting of pressure is limited, otherwise will cause more large economy loss.Therefore each side is devoted to study the culture transferring problem of microbial fermentation culture transferring device in the industry, to enhance productivity.
The utility model content
The purpose of this utility model provides a kind of microbial fermentation culture transferring device, under sufficient oxygen supply condition, improve culture transferring speed, impaired or dead because of anoxic in the culture transferring process with the microbial strains that avoids vigorous growth, thereby effectively shorten fermentation period, improve fermentation production efficiency.
The utility model purpose is achieved by the following technical solution:
The utility model microbial fermentation culture transferring device, comprise seeding tank, fermentor tank, sterile air plenum system, liquid material conveying tube, valve and electric blender, between seeding tank and seeding tank, between seeding tank and the fermentor tank, between fermentor tank and the fermentor tank, be communicated with through the liquid material conveying tube, be characterized between seeding tank and seeding tank, in the liquid material conveying tube between seeding tank and the fermentor tank, between fermentor tank and the fermentor tank partly or all be in series with the liquid material conveying pump.
The utility model design innovation, owing to be in series with the liquid material conveying pump respectively in the liquid material conveying tube between seeding tank and seeding tank, seeding tank and fermentor tank, fermentor tank and fermentor tank, so can make seeding tank or prime fermentor tank at about 0.6Kg/cm 2Modest pressure under, remain the sterile air system and supply with sterile air continuously to seeding tank or prime fermentor tank, and can keep this jar under the unobstructed condition of sterile air, to carry out kind of liquid shifting, realize shifting at competent oxygen supply condition at the bacterial classification and the tunning of vigorous growth thereby just make in this jar, avoided because of the fermented bacterium weak and dead loss that causes that anoxic causes in the culture transferring process, therefore, adopt the design's device, shortened the cycle of fermenting process, significantly improve fermentation production efficiency, and reduced production cost.
Description of drawings
Fig. 1 the utility model structural representation
Fig. 2 the utility model seeding tank-second order fermentation jar is implemented synoptic diagram
Fig. 3 prior art microbial fermentation culture transferring device synoptic diagram
Embodiment
How now further specify the utility model in conjunction with the accompanying drawings implements:
According to technical solutions of the utility model, can be in conjunction with the particular case of the bacterial classification quantity of microbial fermentation culture transferring, device size, zymotechnique step, in the liquid material conveying tube between seeding tank and seeding tank, between seeding tank and the fermentor tank, between fermentor tank and the fermentor tank partly or all be in series with the liquid material conveying pump.
Example 1
As shown in Figure 1, the utility model microbial fermentation culture transferring device comprises seeding tank 1, fermentor tank 2, sterile air plenum system K, liquid material conveying tube B, valve F and electric blender M, be communicated with through liquid material conveying tube B between seeding tank 1 and the fermentor tank 2, be in series with liquid material conveying pump 3 among the liquid material conveying tube B between seeding tank 1 and fermentor tank 2.
During the technological condition for fermentation bottom fermentation of microbial strains in seeding tank 1, remain sterile air plenum system K and supply with sterile air continuously to seeding tank 1, after microbial strains growth and maturity in seeding tank 1 are some amount mycelia, bacterium amount, need the kind liquid of growth and maturity in the seeding tank 1 is delivered to fermentor tank 2 by pipe connecting B by processing requirement, at this moment, the sterile air outlet valve F appropriateness of seeding tank 1 is turned down, show, keep jar about 0.6Kg/cm of internal pressure by tensimeter Y 2, by working specification, start the liquid material conveying pump 3 that is connected among the liquid material conveying tube B simultaneously, by liquid material conveying pump 3, quicken to realize that mature strains is promptly quickened " culture transferring " to the transfer of fermentor tank 2 in the seeding tank 1.In this culture transferring process, because seeding tank 1 inherent about 0.6Kg/cm 2Modest pressure under, remain the K of sterile air system to seeding tank 1 supply and unobstructed continuously, make the dissolved oxygen abundance of planting in the seeding tank 1 in the liquid, keep the vigorous growth impetus by seeding tank culture transferring to the bacterial classification in the fermentor tank 2, and in fermentor tank 2, further finish fermentation, so shortened the fermenting process production cycle, reduced production cost, improved fermentation production efficiency.
Example 2
As shown in Figure 2, this microbial fermentation culture transferring device comprises seeding tank 1, fermentor tank 2-1, fermentor tank 2-2, sterile air plenum system K, liquid material conveying tube B, valve F and electric blender M between seeding tank 1 and fermentor tank 2-1, be in series with liquid material conveying pump 3-1 and liquid material conveying pump 3-2 among the liquid material conveying tube B between fermentor tank 2-1 and the fermentor tank 2-2 respectively, seeding tank 1 is identical with example 1 embodiment with culture transferring between the fermentor tank 2-1, then, kind liquid fermentation in fermentor tank 2-1 extremely to a certain degree, need to shift kind of liquid so that when further fermenting to fermentor tank 2-2, identical with aforementioned operation, promptly appropriateness is closed the sterile air outlet valve F1 of small fermentor 2-1, show by tensimeter Y1, keep jar about 0.6Kg/cm of internal pressure 2, by working specification, start the liquid material conveying pump 3-1 that is connected among the liquid material conveying tube B simultaneously, by liquid material conveying pump 3-1, make the fermentation kind liquid in the fermentor tank 2-1, quicken to enter among the next stage fermentor tank 2-2, through further finishing fermentative production after the fermentation.
The fermentor tank of this microbial fermentation culture transferring device is one or more, is not limited to above example.

Claims (1)

1, a kind of microbial fermentation culture transferring device, comprise seeding tank, fermentor tank, sterile air plenum system, liquid material conveying tube, valve and electric blender, between seeding tank and seeding tank, between seeding tank and the fermentor tank, between fermentor tank and the fermentor tank, be communicated with through the liquid material conveying tube, it is characterized in that between seeding tank and seeding tank, in the liquid material conveying tube between seeding tank and the fermentor tank, between fermentor tank and the fermentor tank partly or all be in series with the liquid material conveying pump.
CN 200820068509 2008-07-17 2008-07-17 Microorganism fermentation transplantation device Expired - Fee Related CN201254567Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200820068509 CN201254567Y (en) 2008-07-17 2008-07-17 Microorganism fermentation transplantation device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200820068509 CN201254567Y (en) 2008-07-17 2008-07-17 Microorganism fermentation transplantation device

Publications (1)

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CN201254567Y true CN201254567Y (en) 2009-06-10

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107299047A (en) * 2017-08-24 2017-10-27 北京艾吾丁医药科技有限公司 A kind of culture transferring control method based on multistage installation for fermenting
CN110656045A (en) * 2019-07-08 2020-01-07 青海华牛生物科技有限公司 Wolfberry enzyme fermentation guiding and conveying system

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107299047A (en) * 2017-08-24 2017-10-27 北京艾吾丁医药科技有限公司 A kind of culture transferring control method based on multistage installation for fermenting
CN110656045A (en) * 2019-07-08 2020-01-07 青海华牛生物科技有限公司 Wolfberry enzyme fermentation guiding and conveying system

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C17 Cessation of patent right
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Granted publication date: 20090610

Termination date: 20110717