CN201148435Y - 带压接种瓶 - Google Patents

带压接种瓶 Download PDF

Info

Publication number
CN201148435Y
CN201148435Y CN 200720187732 CN200720187732U CN201148435Y CN 201148435 Y CN201148435 Y CN 201148435Y CN 200720187732 CN200720187732 CN 200720187732 CN 200720187732 U CN200720187732 U CN 200720187732U CN 201148435 Y CN201148435 Y CN 201148435Y
Authority
CN
China
Prior art keywords
bottle
inoculation
steel pipe
inoculation bottle
pressure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 200720187732
Other languages
English (en)
Inventor
贾洪卫
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN 200720187732 priority Critical patent/CN201148435Y/zh
Application granted granted Critical
Publication of CN201148435Y publication Critical patent/CN201148435Y/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

一种带压接种瓶涉及发酵罐的接种装置。采用的设计方案是：在接种瓶的瓶口有瓶口盖，瓶口盖的内侧安有密封垫，底部安有滤网的钢管从瓶口盖下部穿过，钢管上部一体连通针头，钢管上口封闭，在发酵罐上增设一个安有密封垫的接口。接种瓶为容积0.5～2.0升的耐压不锈钢瓶。钢管的外径小于接种瓶瓶口内径。本实用新型的有益效果是，接种时罐内带正压，杜绝外界细菌进入罐内的机会，不用点火灭菌，对周围空气质量要求不高，省时省力，安全便捷，节约原材料。

Description

带压接种瓶
一、技术领域
本实用新型涉及一种发酵罐的接种装置。
二、背景技术
目前，发酵常规的接种瓶是由玻璃制做的克氏瓶，接种时，在发酵罐接种口套上火圈灭菌，然后，人手拿着克氏瓶往接种口内倾倒菌种液。因罐内是常压，在接种时外界细菌量进入罐内。对操作者的实际技能要求高，且常压玻璃瓶易破损。
三、发明内容
本实用新型的目的是设计一种能杜绝由常压接种可能引起染菌问题的接种瓶。
实现上述目的采用的设计方案是：在接种瓶的瓶口有瓶盖，瓶盖的内侧安有密封垫，底部安有滤网的钢管从瓶盖下部穿过，钢管上部一体连通针头，钢管上口封闭，在发酵罐上增设一个安有密封垫的接口。接种瓶为容积0.5～2.0升的耐压不锈钢瓶。钢管的外径小于接种瓶瓶口内径。
本实用新型的有益效果是，接种时罐内带正压，杜绝外界细菌进入罐内的机会。不用点火灭菌，对周围空气质量要求不高，省时省力，安全便捷，节约原材料。
四、附图说明
图1是带压接种瓶的整体结构示意图。
图2是发酵罐上安装有密封垫接口的结构示意图。
五、具体实施方式
首先制作不锈钢的接种瓶(1)，它容积1.0升左右，耐压不少于2个大气压，在瓶口放一个硅胶密封垫(2)，将不锈钢钢管(5)穿过瓶盖(3)，钢管(5)下口安有不锈钢滤网(6)，上口密闭的钢管一体连通有一个不锈钢空心针头(4)，然后将瓶盖(3)紧密盖在接种瓶(1)瓶口。另外，在体积较大的发酵罐(10)上增设一个铜质丝头(7)，其口部放硅胶密封垫(8)，然后用铜帽(9)将丝头(7)口部封住，铜帽(9)顶面中心开有孔，其孔径比针头(4)外径略大。使用时，首先将接种瓶(1)瓶口部分带在瓶口上，用消毒棉球包好空心针头(4)，把接种瓶(1)放入灭菌柜灭菌后，在灭菌室加入菌种液，然后去掉空心针头(4)的棉球，手持接种罐(1)，针头(4)扎透密封垫(8)与发酵罐(10)相通。使接种瓶(1)与发酵罐(10)压力平衡，然后关闭发酵罐进气阀再打开发酵罐放气阀泄压。使处于高位的菌液在罐压和液位差的作用下，快速流入发酵罐。待菌种压完后，拔出针头(4)拧紧铜帽(9)即可。最后使发酵罐(10)升压到正常状态。

Claims (3)

1、一种带压接种瓶，其特征在于：在接种瓶(1)的瓶口有瓶盖(3)，瓶口盖(3)的内侧安有密封垫(8)，底部安有滤网的钢管(5)从瓶盖(3)下部穿过，钢管(5)上部一体连通针头(4)，钢管(5)上口封闭，在发酵罐(10)上增设一个安有密封垫(8)的接口。
2、根据权利要求1所述的带压接种瓶，其特征在于：接种瓶(1)为容积0.5～2.0升的耐压不锈钢瓶。
3、根据权利要求1所述的带压接种瓶，其特征在于：钢管(5)的外径小于接种瓶(1)瓶口内径。
CN 200720187732 2007-12-18 2007-12-18 带压接种瓶 Expired - Fee Related CN201148435Y (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200720187732 CN201148435Y (zh) 2007-12-18 2007-12-18 带压接种瓶

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200720187732 CN201148435Y (zh) 2007-12-18 2007-12-18 带压接种瓶

Publications (1)

Publication Number Publication Date
CN201148435Y true CN201148435Y (zh) 2008-11-12

Family

ID=40116202

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200720187732 Expired - Fee Related CN201148435Y (zh) 2007-12-18 2007-12-18 带压接种瓶

Country Status (1)

Country Link
CN (1) CN201148435Y (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104560693A (zh) * 2015-01-29 2015-04-29 浦城正大生化有限公司 大容量菌液接种装置及采用该装置的压差注射接种方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104560693A (zh) * 2015-01-29 2015-04-29 浦城正大生化有限公司 大容量菌液接种装置及采用该装置的压差注射接种方法

Similar Documents

Publication Publication Date Title
CN201148435Y (zh) 带压接种瓶
CN101480135B (zh) 一种食用菌液体菌种培养装置
CN202369585U (zh) 一种厌氧接种罐
CN202322844U (zh) 移种装置
CN206970614U (zh) 一种微生物发酵罐接种装置
CN2885869Y (zh) 液体菌种生产用简易发酵装置
CN2579900Y (zh) 食用菌深层培养发酵罐药液消毒进、排气装置
CN201439008U (zh) 便携式手动负压引流器
CN208733088U (zh) 样本密度分离液保存装置
CN207998137U (zh) 一种蒸汽灭菌包装瓶
CN202786227U (zh) 一种厌氧发酵装置
CN209669167U (zh) 一种便于排气的葡萄酒发酵罐
CN208505993U (zh) 气相色谱仪测定气体组分进样装置
CN207079230U (zh) 一种红曲霉发酵盒
CN203128570U (zh) 反硝化鉴定预制管
CN2436427Y (zh) 啤酒桶进气阀
CN205323315U (zh) 压力式无菌液体过滤器
CN206173302U (zh) 一种黄酒发酵及程度检测装置
CN219950943U (zh) 一种白酒食品安全检测用过滤装置
CN203096063U (zh) 一种带压接种罐
CN214608399U (zh) 一种吨料袋制备发酵饲料的封口装置
CN204468772U (zh) 一种接种针消毒装置
CN211157455U (zh) 一种高压灭菌锅
CN219824233U (zh) 一种插拔式细胞瓶
CN202322311U (zh) 医用药液分装罐

Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20081112

Termination date: 20101218