CN201101949Y - Numerator purifying pole with purification film fixing structure - Google Patents
Numerator purifying pole with purification film fixing structure Download PDFInfo
- Publication number
- CN201101949Y CN201101949Y CNU2007201817053U CN200720181705U CN201101949Y CN 201101949 Y CN201101949 Y CN 201101949Y CN U2007201817053 U CNU2007201817053 U CN U2007201817053U CN 200720181705 U CN200720181705 U CN 200720181705U CN 201101949 Y CN201101949 Y CN 201101949Y
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- China
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- purification
- ridge structure
- molecule
- cylinder
- purification column
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Abstract
The utility model provides a molecule purification column, which can simplify processing course and increase purification efficiency. A separate type fixed ring is cancelled on the inner wall of the column body of the purification column. The upper part of the installing position of a purification film is provided with an apophysis structure used for fixing the purification film. The apophysis structure is integrated with the column body as a whole. The number of the apophysis structure can be one or more, and the apophysis structures are in an annular distribution. The inner side edge of the lengthwise section presents an arc shape, and the spophysis height is 1 percent to 10 percent of the caliber of the column body at the installing position of the purification film, which is convenient to break away from the die during plastic processing, and is also convenient to slip the purification film. The molecule purification leaves out a single fixed ring, simplifies the technics of manufacturing and film installation, and furthermore, avoids difference among columns due to imprecision of the installing position of the fixed ring, at the same time avoids the possibility that liquid remains on the fixed ring during the using course.
Description
Technical field
The utility model belongs to biological technical field, particularly a kind of molecule purification column.
Background technology
The molecule purification column that grows up according to the reversible binding characteristic between special adsorbing medium and the biomolecule is widely used in fields such as biological study, production, detection, for example, glass fiber filter is filled into can be used in the plastics cylinder separating and purification of nucleic acid molecules.At present, commercially available purification column comprises cylinder and is installed on the interior purification membrane of cylinder that purification membrane fixes by a separate type retainer ring.If without retainer ring, only depend on the frictional force of purification membrane and inboard wall of cylinder, purification membrane is easy to come off.In process of production, in the cylinder of the hollow of at first purification membrane being packed into, and then fill in retainer ring and fixed, therefore, the mounting process complexity.Because the needs of retainer ring self mechanical strength, the size of retainer ring is bigger, and its longitudinal section is length and width all greater than the rectangle of 1mm (referring to Fig. 1 a).Such purification column in use, liquid remains in the space between retainer ring and the inboard wall of cylinder easily, to such an extent as to reduce purification effect.
Summary of the invention
In order to overcome deficiency of the prior art, the utility model provides a kind of molecule purification column that process can improve purification efficiency again of simplifying.
The technical solution of the utility model is, the retainer ring of cancellation separate type, and on the purification column inboard wall of cylinder, the top of purification membrane installation site has the ridge structure that is used for fixing purification membrane, and this ridge structure and cylinder are integrally formed.
The ridge structure of above-mentioned alternative retainer ring can distribute usually in the form of a ring for one or more.So-called protuberance is for the position of inboard wall of cylinder contact purification membrane, such as, the raised points (as 3,4,5 or 6 projectioies, referring to Fig. 3 a and 3b) of a bulge loop (referring to Fig. 2 a and 2b) or a circle dispersion can be set at the installed position that is equivalent to traditional retainer ring; Also can do the wall of cylinder thicker, form a circle depression (referring to Fig. 4) at the position that purification membrane is installed, the cylinder part on the depression just be equivalent to annular projection.The function of these ridge structure is same as the retainer ring of packing into separately.
Break away from mould during for ease of plastic processing, also be convenient to fill in purification membrane, the inside edge of the longitudinal section of ridge structure is curved, can be one section circular arc, also can be other comparatively complicated arc (for example shown in Figure 5).The height of protuberance is generally the 1%-10% of dress membranous part position cylinder bore, and at the most frequently used miniprep post shapes (the cylinder internal diameter is about 7mm), its bump height is that 0.1-0.5mm just is enough to fixedly purification membrane.In the purification membrane installation process, make it cross ridge structure as long as purification membrane pushed slightly, purification membrane just is fixed in the cylinder, need not add retainer ring again.Because this ridge structure is an one of cylinder integral body, size can be littler than independent retainer ring, and the ridge structure surface can do very gently with respect to the angle on inboard wall of cylinder surface, thereby in use liquid that can not is detained herein.
Molecule purification column of the present utility model has saved independent retainer ring, not only simplified manufacturing process, and simplified the technology of dress film, the operation of retainer ring is filled in omission, thereby avoided because the post that retainer ring installation site inaccuracy is brought and the difference of intercolumniation, avoided liquid in the use residual possible on retainer ring simultaneously.
Description of drawings
Fig. 1 a is the longitudinal sectional view of existing purification column;
Fig. 1 b is the cutaway view of walking crosswise that existing purification column installs and fixes the ring place.
Fig. 2 a is the longitudinal sectional view of the utility model embodiment 1 purification column;
Fig. 2 b is the cutaway view of walking crosswise at the ridge structure of purification column cylinder shown in Fig. 2 a position.
Fig. 3 a is the longitudinal sectional view of the utility model embodiment 2 purification columns;
Fig. 3 b is the cutaway view of walking crosswise at the ridge structure of purification column cylinder shown in Fig. 3 a position.
Fig. 4 is the longitudinal sectional view of the utility model embodiment 3 purification columns.
Fig. 5 is the longitudinal sectional view of the utility model embodiment 4 purification columns.
Among the figure:
1---purification column cylinder 2---purification membrane 3---separate type retainer ring 4---ridge structure
The specific embodiment
Below in conjunction with accompanying drawing, further specify the utility model by embodiment, but limit scope of the present utility model never in any form.
Shown in Fig. 2 a and 2b, being used to replace the ridge structure 4 that has separate type retainer ring function now is to be positioned at bulge loop on purification column cylinder 1 inwall, and purification membrane 2 is fixed in the cylinder, and this ridge structure longitudinal cross-section is semicircle, and radius is about 0.3mm.Than the existing purification column of the band separate type retainer ring 3 shown in Fig. 1 a and the 1b, the ring-type ridge structure and the cylinder of the purification column of present embodiment are integrally formed, have simplified manufacturing and mounting process, and when having avoided using liquid residue on retainer ring may.
In the molecule purification column shown in Fig. 3 a and the 3b, ridge structure 4 is six hemispheric projectioies, be evenly distributed in circlewise on the purification column inwall, the radius of each hemispherical projections is about 0.3mm, purification membrane 2 just is stuck in the projection below of these spot distribution, and result of use is similar with whole ring-type ridge structure.
Molecule purification column as shown in Figure 4, the position that purification membrane 2 is installed are circle depressions that forms on the inboard wall of cylinder, and with respect to this depression, the inwall on cylinder top is exactly a ridge structure 4, and its function is identical with the bulge loop of embodiment 2.
Molecule purification column as shown in Figure 5, from the vertical section, ridge structure 4 inside edges are not simple circular arc, but form a gentle slope at an upper portion thereof, carry out the transition to gently on cylinder 1 inwall.
The foregoing description and accompanying drawing are of the present utility model giving an example, and are not to be used to limit the utility model, and it will be appreciated by those skilled in the art that: in not breaking away from spirit and scope of the present utility model, various replacements, variation and modification all are possible.Therefore, the present invention's protection domain of the present utility model is looked the claim scope and is defined.
Claims (8)
1. a molecule purification column comprises cylinder and purification membrane, it is characterized in that: on inboard wall of cylinder, the top of purification membrane installation site has the ridge structure that is used for fixing purification membrane, and described ridge structure and cylinder are integrally formed.
2. molecule purification column according to claim 1 is characterized in that: described ridge structure is one or more.
3. molecule purification column according to claim 2 is characterized in that: described ridge structure is an outstanding circulus in position with respect to inboard wall of cylinder contact purification membrane.
4. molecule purification column according to claim 2 is characterized in that: described ridge structure is the ring-shaped distributed projection of a plurality of dispersions.
5. molecule purification column according to claim 4 is characterized in that: described projection is a hemispherical.
6. according to the described molecule purification column of arbitrary claim in the claim 2～4, it is characterized in that: the inside edge of the longitudinal section of described ridge structure is curved.
7. according to the described molecule purification column of arbitrary claim in the claim 2～5, it is characterized in that: the bump height of described ridge structure is the 1%-10% of purification membrane installation position cylinder bore.
8. molecule purification column according to claim 6 is characterized in that: the bump height of described ridge structure is the 1%-10% of purification membrane installation position cylinder bore.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007201817053U CN201101949Y (en) | 2007-10-19 | 2007-10-19 | Numerator purifying pole with purification film fixing structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007201817053U CN201101949Y (en) | 2007-10-19 | 2007-10-19 | Numerator purifying pole with purification film fixing structure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201101949Y true CN201101949Y (en) | 2008-08-20 |
Family
ID=39948967
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007201817053U Expired - Fee Related CN201101949Y (en) | 2007-10-19 | 2007-10-19 | Numerator purifying pole with purification film fixing structure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201101949Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104368242A (en) * | 2014-11-06 | 2015-02-25 | 海门市优耐特实验器材发展有限公司 | DNA purification column film-mounting tool and film-mounting method thereof |
-
2007
- 2007-10-19 CN CNU2007201817053U patent/CN201101949Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104368242A (en) * | 2014-11-06 | 2015-02-25 | 海门市优耐特实验器材发展有限公司 | DNA purification column film-mounting tool and film-mounting method thereof |
| CN104368242B (en) * | 2014-11-06 | 2016-08-24 | 海门市优耐特实验器材发展有限公司 | DNA purification column dress film instrument and dress film method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: KAYOUDI BIOTECHNOLOGY (BEIJING) CO., LTD. Free format text: FORMER OWNER: GINGKO BIOTECH (BEIJING) CO., LTD. Effective date: 20130808 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20130808 Address after: 100085, Beijing, Haidian District Road No. 36, five, room 506 Patentee after: COYOTE BIOSCIENCE CO., LTD. Address before: 100085, Beijing, Haidian District Road No. 36, five, Room 501 Patentee before: Gingko Biotech (Beijing) Co., Ltd. |
|
| ASS | Succession or assignment of patent right |
Owner name: GINGKO BIOTECH (BEIJING) CO., LTD. Free format text: FORMER OWNER: KAYOUDI BIOTECHNOLOGY (BEIJING) CO., LTD. Effective date: 20140512 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20140512 Address after: 100085, Beijing, Haidian District Road No. 36, five, room 511 Patentee after: Gingko Biotech (Beijing) Co., Ltd. Address before: 100085, Beijing, Haidian District Road No. 36, five, room 506 Patentee before: COYOTE BIOSCIENCE CO., LTD. |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080820 Termination date: 20151019 |
|
| EXPY | Termination of patent right or utility model |