CN104368242B - DNA purification column dress film instrument and dress film method thereof - Google Patents
DNA purification column dress film instrument and dress film method thereof Download PDFInfo
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- CN104368242B CN104368242B CN201410616675.9A CN201410616675A CN104368242B CN 104368242 B CN104368242 B CN 104368242B CN 201410616675 A CN201410616675 A CN 201410616675A CN 104368242 B CN104368242 B CN 104368242B
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- gun
- push rod
- cutter head
- dress film
- dna purification
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Abstract
The invention discloses DNA purification column dress film instrument, it includes the body of a gun, cutter head and push rod, body of a gun upper end connects handle, body of a gun bottom connects cutter head, arranging spring and push rod in the body of a gun, spring is placed on push rod, and body of a gun surface has a groove, install in groove and push away torsion screw, push away torsion screw end and act on push rod.The invention also discloses the method using DNA purification column dress film instrument to carry out filling film.Using the dress film instrument of the present invention, simple in construction, semi-automatic degree is high, only need to promote or dial rotation pushing away torsion screw, cut, press mold speed fast, filter membrane, trim ring are secured firmly to bottom filter tube, waste product will not be produced, qualification rate is high.
Description
Technical field
The present invention relates to a kind of DNA purification column dress film instrument.
The invention still further relates to a kind of DNA purification column dress film method.
Background technology
A large amount of plasmid DNA extracted typically need to be further purified, and needs to use DNA purification pipe in purge process.Existing
DNA purification pipe, including filter tube, filter tube bottom has a funnelform outlet, needs to be manually placed into bottom filter tube
Trim ring and filter membrane, Manual Speed is slow, fixing loosely, the percent defective of generation is high.Accordingly, it is desirable to provide a kind of new technical scheme
Solve the problems referred to above.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of quick, DNA purification column dress film instrument of fixation.
The present invention also needs to solve the technical problem that and is to provide a kind of DNA purification column dress film method.
For solving above-mentioned technical problem, the DNA purification column dress film instrument of the present invention, it includes the body of a gun, cutter head and push rod, institute
Stating body of a gun upper end and connect handle, described body of a gun bottom connects cutter head, arranges spring and push rod, described spring in the described body of a gun
Being placed on push rod, described body of a gun surface has a groove, installs in described groove and pushes away torsion screw, described in push away torsion screw end and act on
On push rod.
Described push rod is divided into bigger diameter end and miner diameter end, described spring to be placed on miner diameter end, described bigger diameter end has for pushing away torsion
The hole that screw is assigned.
Described body of a gun surface is assigned and is pushed away the groove turning round screw, and described groove includes along the longitudinal part in body of a gun direction with along body of a gun cylindrical
The curved portions that perimeter surface ring sets.
The described body of a gun is connected by septum screw with cutter head.
The described body of a gun is threadeded with handle.
Using above-mentioned dress film instrument to carry out DNA purification column dress film, this dress film method comprises the following steps:
A, when push rod returns state, cutter head is to filter membrane, semicircle rotating down pressing, and the film automatic safety cut is at cutter head mouth
Portion；
B, cutter head alignment needs to fill the filter tube mouth of pipe of film, pushes away and turns round the filter membrane that screw makes push rod release cut, enters
Enter filter tube inner bottom part；
C, group rotation push away and turn round the stationary state after screw makes push rod keep release, are used for press-fiting filter membrane, trim ring；
D, press trim ring after, reversely rotation pushing turns round screw, and push rod automatically resets under the action of the spring, continues next step and moves
Make.
Use the dress film instrument of the present invention, cut, press mold speed fast, simple in construction, semi-automatic degree is high, by filter membrane,
Trim ring is secured firmly to bottom filter tube, will not produce waste product, and qualification rate is high.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention.
Fig. 2 be the present invention cut membrane stage schematic diagram.
Fig. 3 is the press mold view of the present invention.
Fig. 4 is the body of a gun structural representation of the present invention.
Fig. 5 is the pusher structure schematic diagram of the present invention.
Fig. 6 is the structural representation of DNA filter tube.
Wherein: 1, push away torsion screw, 2, handle, 3, pad, 4, cutter head, 5, the body of a gun, 6, push rod, 61, bigger diameter end, 62, path
End, 63, hole, 7, spring, 8, groove, 81, longitudinal part, 82, curved portions, 9, filter tube, 91, lid, 92, filter tube suitable for reading, 93, pressure
Circle, 94, filter membrane, 95, filter tube outlet.
Detailed description of the invention
In order to deepen the understanding of the present invention, below in conjunction with embodiment and accompanying drawing, the invention will be further described, should
Embodiment is only used for explaining the present invention, is not intended that the restriction to protection scope of the present invention.
As it is shown in figure 1, the DNA purification column dress film instrument of the present invention, it includes the body of a gun 5, cutter head 4 and push rod 6, on the body of a gun 5
End thread connects handle 2, and the threaded cutter head of pad 34 is passed through in the body of a gun 5 bottom, arranges spring 7 and push rod 6 in the body of a gun 5,
Spring 7 is placed on push rod 6, and the body of a gun 5 surface has a groove 8, installs and push away torsion screw 1 in groove 8, pushes away torsion screw 1 end and acts on
On push rod 6.
Wherein, pad 3 is set between the body of a gun 5 bottom and cutter head 4 upper surface, pad 3 arrange number, determine push rod 6
Releasing the length of the edge of a knife, the material filling film different is how many.
Shown in Fig. 5, push rod 6 is divided into bigger diameter end 61 and miner diameter end 62, and spring 7 is placed on miner diameter end 62, and spring 7 is supported by cutter head 4
In the body of a gun 5 because cutter head 4 be shaped as sharp cone distal, bigger diameter end 61 has and turns round the hole 63 assigned of screw 1 for pushing away.
Shown in Fig. 4, the body of a gun 5 surface is assigned and is pushed away the groove 8 turning round screw 1, and groove 8 includes the longitudinal part 81 along the body of a gun 5 direction and edge
The curved portions 82 that the body of a gun 5 external peripheral surface ring sets.Push away and turn round screw 1 at the longitudinal part 81 of its groove 8 toward drop-down, then draw in curved portions
82, push away torsion screw 1 active force the most at last and act on push rod 6 so that push rod 6 is released in the body of a gun 5.
With reference to Fig. 6, use the dress film instrument of the present invention, carry out DNA filter tube filling film, filter tube suitable for reading 92 lid 91
Covering, filter tube outlet 95 is shaped like funnel, stacks filter membrane 94, trim ring 93 in filter tube 9 successively.Push rod 6 exposes
Position length, determines the depth that trim ring 93 is pressed in pipe, is suitable for different filter membranes, 6 length exposed of push rod, and corresponding is thin
Film；It is short that push rod 6 exposes, and corresponding is thick film.
Referring to figs. 2 and 3, DNA purification column dress film instrument is cut, the method for press mold:
A, when push rod 6 returns state, cutter head 4 is to filter membrane 94, semicircle rotating down pressing, and the film automatic safety cut is at cutter
4 oral areas；
B, cutter head 4 alignment needs to fill filter tube 9 mouth of pipe of film, pushes away and turns round the filter membrane that screw 1 makes push rod 6 release cut
94, enter filter tube 9 inner bottom part；
C, group rotation push away and turn round the stationary state after screw 1 makes push rod 6 keep release, are used for press-fiting filter membrane 94, trim ring 93；
D, press trim ring 93 after, reversely rotation pushing turns round screw 1, and push rod 6 automatically resets under the effect of spring 7, under continuation
One step action.
Using the dress film instrument of the present invention, simple in construction, semi-automatic degree is high, only need to promote or dial rotation pushing away torsion screw 1,
Cut, press mold speed fast, filter membrane 94, trim ring 93 are secured firmly to bottom filter tube 9, waste product will not be produced, qualification rate is high.
Claims (4)
1.DNA purification column dress film instrument, it is characterised in that: it includes the body of a gun, cutter head and push rod, and described body of a gun upper end connects handle
Hands, described body of a gun bottom connects cutter head, arranges spring and push rod in the described body of a gun, and described spring is placed on push rod, described rifle
Body surface has a groove, installs in described groove and pushes away torsion screw, described in push away torsion screw end and act on push rod；Described push rod divides
For bigger diameter end and miner diameter end, described spring is placed on miner diameter end, described bigger diameter end has and turns round, for pushing away, the hole that screw is assigned；Described groove
Including the longitudinal part along body of a gun direction and the curved portions that sets along body of a gun external peripheral surface ring.
DNA purification column the most according to claim 1 dress film instrument, it is characterised in that: the described body of a gun and cutter head pass through pad
Threaded.
DNA purification column the most according to claim 1 dress film instrument, it is characterised in that: the described body of a gun is threadeded with handle.
4. the dress film method of film instrument is filled according to the DNA purification column described in any one of claim 1-3, it is characterised in that: include
Following steps:
A, when push rod returns state, cutter head is to filter membrane, semicircle rotating down pressing, and the film automatic safety cut is at cutter head oral area；
B, cutter head alignment needs to fill the filter tube mouth of pipe of film, pushes away and turns round the filter membrane that screw makes push rod release cut, entered
Chimney filter inner bottom part；
C, group rotation push away and turn round the stationary state after screw makes push rod keep release, are used for press-fiting filter membrane, trim ring；
D, press trim ring after, reversely rotation pushing turns round screw, and push rod automatically resets under the action of the spring, continues next step action.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410616675.9A CN104368242B (en) | 2014-11-06 | 2014-11-06 | DNA purification column dress film instrument and dress film method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410616675.9A CN104368242B (en) | 2014-11-06 | 2014-11-06 | DNA purification column dress film instrument and dress film method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104368242A CN104368242A (en) | 2015-02-25 |
| CN104368242B true CN104368242B (en) | 2016-08-24 |
Family
ID=52547588
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410616675.9A Active CN104368242B (en) | 2014-11-06 | 2014-11-06 | DNA purification column dress film instrument and dress film method thereof |
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| Country | Link |
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| CN (1) | CN104368242B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201101949Y (en) * | 2007-10-19 | 2008-08-20 | 金银杏生物科技(北京)有限公司 | Numerator purifying pole with purification film fixing structure |
| CN203440270U (en) * | 2013-08-02 | 2014-02-19 | 中山康方生物医药有限公司 | Dismountable protein purification column |
| CN203745435U (en) * | 2013-12-24 | 2014-07-30 | 烟台青云仪器设备有限公司 | Special sleeve centrifugal QuEChERS purifier |
| CN204220042U (en) * | 2014-11-06 | 2015-03-25 | 海门市优耐特实验器材发展有限公司 | DNA purification column dress film instrument |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8414846B2 (en) * | 2007-09-11 | 2013-04-09 | University Of Florida Research Foundation, Inc. | Devices and methods for the collection and detection of substances |
-
2014
- 2014-11-06 CN CN201410616675.9A patent/CN104368242B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201101949Y (en) * | 2007-10-19 | 2008-08-20 | 金银杏生物科技(北京)有限公司 | Numerator purifying pole with purification film fixing structure |
| CN203440270U (en) * | 2013-08-02 | 2014-02-19 | 中山康方生物医药有限公司 | Dismountable protein purification column |
| CN203745435U (en) * | 2013-12-24 | 2014-07-30 | 烟台青云仪器设备有限公司 | Special sleeve centrifugal QuEChERS purifier |
| CN204220042U (en) * | 2014-11-06 | 2015-03-25 | 海门市优耐特实验器材发展有限公司 | DNA purification column dress film instrument |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104368242A (en) | 2015-02-25 |
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| C10 | Entry into substantive examination | ||
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| C14 | Grant of patent or utility model | ||
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: DNA purification column film-mounting tool and film-mounting method thereof Effective date of registration: 20171113 Granted publication date: 20160824 Pledgee: Haimen City Science and Technology Park Service Center Pledgor: HAIMEN UNITED LABORATORY EQUIPMENT DEVELOPMENT CO., LTD. Registration number: 2017980000455 |