CN205170837U - Purification device - Google Patents
Purification device Download PDFInfo
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- CN205170837U CN205170837U CN201520881725.6U CN201520881725U CN205170837U CN 205170837 U CN205170837 U CN 205170837U CN 201520881725 U CN201520881725 U CN 201520881725U CN 205170837 U CN205170837 U CN 205170837U
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- pore
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- filtering membrane
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Abstract
The utility model discloses a purification device, including collecting post, filtration membrane and card membrane element. Collect the post and seted up the lumen along the axial. Filtration membrane sets up nucleic acid is adsorbed in order to filter impurity in bottom in the lumen the card membrane element set up just set up in the lumen and be in the filtration membrane top is with the restriction filtration membrane's activity. Card membrane element has the through -hole of seting up along the axial and arrives for impurity and nucleic acid flow filtration membrane is last. The utility model discloses the facility is retrencied, is assembled to the purification device structure, and just the purification is efficient.
Description
Technical field
The utility model relates to treater in biology field, particularly a kind of purification devices.
Background technology
Nucleic acid is the carrier of genetic information, is most important biological information molecule, is also object and the instrument of many life sciences.
At occurring in nature, nucleic acid is present in the nucleus of various biology, plastosome, chloroplast(id), also can be present in the tenuigenin of some cell with unbound state.The research of the nucleic acid in these materials is be unable to do without to the process of nucleic acid wherein being carried out to Extraction and separation purifying.In vitro, nucleic acid also through amplification and can modify the process such as processing, in these links, also needs to use a large amount of to purge process.
Utility model content
The purpose of this utility model is to provide a kind of convenient purification devices making and use, and by flexible design, makes it only to need in process of production to be replaced by simple parts to adapt to different materials and the needs of sample.
For realizing above object, the utility model is achieved through the following technical solutions:
A kind of purification devices, is characterized in that, comprising:
Collect post, this collection post offers tube chamber vertically;
Filtering membrane, this filtering membrane be arranged on described intraluminal bottom with impurity screening adsorbs nucleic acid;
Card membrane, this card membrane to be arranged in described tube chamber and to be arranged on to limit the activity of described filtering membrane above described filtering membrane,
Described card membrane has the through hole offered vertically and flow on described filtering membrane for impurity and nucleic acid.
Preferably, described card membrane is ring washer; Described card membrane has the cross-sectional area identical with described filtering membrane and cross-sectional profiles.
Preferably, described card membrane comprises down leg and upper pipe portion, and described upper pipe portion is arranged in described down leg; Described upper pipe portion has the first pore, and described down leg has the second pore; Described first pore and the second pore head and the tail are connected to form described through hole; Described second pore has the caliber being less than described first pore; Described filtering membrane is contained in described second pore.
Preferably, described filtering membrane and described card membrane are arranged in the tube chamber of described collection post removably.。
Preferably, the bottom of described second pore is provided with the first right-angled intersection barrier rib, the first ring-shaped step or multiple first boss to prevent described filtering membrane landing.
Preferably, described through hole comprises the first pore and the second pore that are interconnected, and described first pore is opened in described upper pipe portion, and described second pore is opened in described down leg; Described filtering membrane has the cross-sectional area being less than described upper pipe portion; Described filtering membrane is contained in described second pore; And between the described filtering membrane intraluminal bottom that is set to be limited in described collection post and described second pore.
Preferably, the bottom of described second pore is provided with the first right-angled intersection barrier rib, the first ring-shaped step or multiple first boss to prevent described filtering membrane landing.
Preferably, the inside sidewalls of described second pore is projecting the first stop portion; Described first stop portion is set to the first annular fin or at least two projections; Described filtering membrane is set to be limited between described first stop portion and the bottom of the second pore, and described filtering membrane be set to when stressed can taking out to be arranged in this second pore or in this second pore axially beyond described first stop portion along described collection post; And/or the inside sidewalls of described second pore is set to abut against with described filtering membrane, can moves in the second pore when described filtering membrane is arranged on stressed and take out to be arranged in this second pore or in this second pore.
Preferably, the inside sidewalls of the tube chamber of described collection post is projecting the second stop portion; Described card membrane is set to be limited between described second stop portion and the bottom of described through hole, and described card membrane be set to when stressed can taking out to be arranged in this second pore or in this second pore axially beyond described second stop portion along described collection post; And/or the inside sidewalls of the tube chamber of described collection post is set to abut against with described card membrane, can moves in the second pore when described filtering membrane is arranged on stressed and take out to be arranged in this second pore or in this second pore.
Preferably, described second stop portion is set to the second ring-type raised line, the second annular fin or the second annular boss; Elastic deformation is there is and crosses described second stop portion to make described card membrane can cross when stressed at least one in this second stop portion and described card membrane when stressed.
Preferably, the tube chamber of described collection post is set to circular cylindrical cavity; The cross section of described card membrane is set to circle; Described filtering membrane is set to cylindrical.
Preferably, the bottom of the tube chamber of described collection post is set to taper shape, or the bottom inside of this tube chamber is provided with the second right-angled intersection barrier rib, the second ring-shaped step or multiple second boss to prevent described filtering membrane or the landing of described card membrane.
Preferably, described purification devices can be used for purification of nucleic acid.
Compared with prior art, the utility model purification devices structure is simplified, is assembled facility and purification efficiency is high.Preferably, the utility model purification devices, effect is good, and can the filtering membrane of the different size of flexible adaptation, to adapt to the needs of different sample.Purification devices of the present utility model, by changing card membrane, can adapt to the film body material of the filtering membrane of different area neatly.Especially the film body material of small area, can adapt to the wash-out that less solution carries out nucleic acid.The utility model purification devices in sample size few or nucleic acids in samples concentration is lower, can significantly improve nucleic acid organic efficiency and improve reclaim concentration.
Accompanying drawing explanation
Fig. 1 is the structural representation of the embodiment one of the utility model purification devices.
Fig. 2 is the three-dimensional exploded view of the purification devices of Fig. 1.
Purification devices in Fig. 3 Fig. 1 is along the sectional view of C1-C1 line.
Fig. 4 is the enlarged diagram at A place in Fig. 3.
Fig. 5 is the structural representation of the embodiment two of the utility model purification devices.
Fig. 6 is the perspective exploded view of the purification devices in Fig. 2.
Fig. 7 is the sectional view of the purification devices in Fig. 5 along C2-C2 line.
Fig. 8 is the enlarged diagram at the B place in Fig. 7.
Fig. 9 is the structural representation of the card membrane in Fig. 6.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described in detail:
Embodiment one:
As depicted in figs. 1 and 2, it is structural representation and the three-dimensional exploded view of the purification devices 101 of the utility model embodiment one.Described purification devices 101 comprises collects post 10, filtering membrane 20 and card membrane 30.It should be noted that, " axis " mentioned in the utility model refers to axis along described collection post 10 or length direction." on ", " top ", " upper end " or " top " and D score, " bottom ", " lower end " or " bottom " be relative.It is mentioned in the utility model that " cross section " refers to the cross section perpendicular to the axis of collecting post 10.In the utility model, the relatively described filtering membrane 20 of described card membrane 30 is arranged on the upper direction of this filtering membrane 20.
See also Fig. 3 and Fig. 4, described collection post 10 can adopt mould to make.Described collection post 10 is all general for different application, and mates with general 1.5ml centrifuge tube or 2ml centrifuge tube.Described collection post 10 offers tube chamber 11 vertically.The shape of described tube chamber 11 and the technology of specification all known by those skilled in the art.In the present embodiment, described tube chamber 11 is set to cylindrical tube chamber.The bottom 3011 of described tube chamber 11 limits described filtering membrane 20 to prevent this filtering membrane 20 landing.Preferably, the second right-angled intersection barrier rib or the second ring-shaped step (not shown) is provided with inside the bottom 3011 in the tube chamber 11 of described collection post 10 to prevent described filtering membrane 20 or card membrane 30 landing.In the present embodiment, the bottom 3011 in the tube chamber 11 of described collection post 10 is provided with multiple second boss 112 to support described filtering membrane 20.The middle part that this boss 112 is arranged towards tube chamber 11 extends.
It is envisioned that described tube chamber 11 has both ends open.In the present embodiment, the bottom of described tube chamber 11 is set to taper shape.This taper shape or doline, as long as can stop that described filtering membrane 20 exits.The bottom of described tube chamber can also extended thrust-augmenting nozzle.In the present embodiment, the outer shape of described collection post 10 is set to cylindrical.The top of described collection post 10 can also be provided with capping (not indicating in figure).This capping can connect, so that dismounting by being flexibly connected sheet.In the present embodiment, described collection post 10 adopts polypropylene material to make integral piece.As preferably, the inside sidewalls of the tube chamber 11 of described collection post 10 is projecting the second stop portion 111.This second stop portion 111 is arranged on the top of described card membrane 30, exits from the upper opening of described collection post 10 to prevent this card membrane 30.Further, described second stop portion 111 can be set to the second ring-type raised line or the second annular boss.Further, described second ring-type raised line, the second annular fin or the second annular boss form along the inner wall surrounding of described tube chamber 11 for one week.There is elastic deformation when second stop portion 111 can be arranged on stressed, to make described card membrane 30 cross this second stop portion 111, thus make described card membrane 30 to exit or to be arranged in this collection post 10 in collection post 10 expediently.In the present embodiment, described second stop portion 111 is set to the second annular fin.When stressed, elastic deformation is there is and crosses described second stop portion 111 to make described card membrane 30 can cross when stressed in this second stop portion 111 with at least one in described card membrane 30.When described tube chamber 11 is cylindrical, the diameter of this cylindrical cross section can be 5 millimeters to 10 millimeters.
Please continue to refer to Fig. 2, Fig. 3 and Fig. 4, described filtering membrane 20 is arranged on bottom 3011 in described tube chamber 11 with impurity screening and adsorbs nucleic acid.The material of described filtering membrane 20 is selected according to filtering requirement.In the present embodiment, described filtering membrane 20 glass fibre element film, thus adsorbs nucleic acid efficiently, and can be convenient to remove the albumen of non-specific adsorption or cell debris etc.Described filtering membrane 20 is set to cylindrical or patty.Described filtering membrane 20 is set to be limited between the bottom 3011 of described tube chamber 11 and described card membrane 30.Described filtering membrane 20 can be set to and described card membrane 30 contacts, abut or interval.
Please continue to refer to Fig. 2, Fig. 3 and Fig. 4, described card membrane 30 to be arranged in described tube chamber 11 and the top being arranged on filtering membrane 20 to limit the activity of described filtering membrane 20, namely described filtering membrane 20 is limited between the bottom 3011 of described tube chamber 11 and this card membrane 30 by described card membrane 30.Described card membrane 30 offers through hole 301 and flows on described filtering membrane 20 for impurity and nucleic acid.Described card membrane 30 can be ring washer.Described card membrane 30 is set to have the cross-sectional area identical with described filtering membrane 20 and cross-sectional profiles.Described card membrane 30 also can adopt polypropylene material to make integral piece.In an embodiment, described card membrane 30 is set to annulus packing ring, and namely described through hole 301 is arranged on the middle part of this rounded washers.When assembling, assembling in order, first filtering membrane 20 being put into the bottom 3011 of the tube chamber 11 collecting post 10, then by described card membrane 30, filtering membrane 20 being fixed.
Illustrate the installation of embodiment one, removing relation below: when mounted, filtering membrane 20, card membrane 30 are put into from the top mouth of pipe of tube chamber 11 one by one, or filtering membrane 20 connects setting (such as being connected by viscose) in the bottom of card membrane 30, then puts into above tube chamber 11 together with card membrane 30 at filtering membrane 20.Again filtering membrane 20, card membrane 30 are crossed described second stop part 111.Described filtering membrane 20 can drop on described boss 112.Described card membrane 30 is for described second stop part 111 stops and can not exit from the top of tube chamber 11.Described filtering membrane 20 is limited between described boss 112 (or bottom of conical tube chamber 11) and described card membrane 30.When dismantling, by instrument or manually external force be applied in card membrane 30, this card membrane 30 is made to cross the second stop part 111 and exit in described tube chamber 11, so that change this card membrane 30.Then, then by filtering membrane 20 take out, so that change this card membrane in described tube chamber 11.
Embodiment two:
Refer to Fig. 5 and Fig. 6, its embodiment two provided for the utility model.In the present embodiment two, described purification devices 102 is from the difference of embodiment one: the card membrane 32 in this embodiment two is different with the card membrane 30 of embodiment one, and the filtering membrane 22 in corresponding embodiment two is with filtering membrane 20 difference in embodiment one.Described card membrane 32 comprises down leg 321 and is arranged on the upper pipe portion 322 in this down leg 321.Described down leg 321 is set to relative with described filtering membrane 22.Described upper pipe portion 322 is set to be limited in described tube chamber 11.Described through hole 301 runs through and is opened in described upper pipe portion 322 and described down leg 321.Described down leg 321 can have the cross-sectional area being less than described upper pipe portion 322, thus realizes the stop mutually with the described filtering membrane 22 of the cross-sectional area being less than or equal to described upper pipe portion 322.Certainly, in the present embodiment two, described filtering membrane 22 is arranged in the second pore 303 of described down leg 321.The cross-sectional area of described down leg 321 also can equal the cross-sectional area in described upper pipe portion 321.
See also Fig. 7 and Fig. 8, preferably described, the bottom in the tube chamber 11 of described collection post 10 is provided with right-angled intersection barrier rib, ring-shaped step or outstanding stop part to prevent the landing of described card membrane 32.Preferably, the bottom 3011 in the tube chamber 11 of described collection post 10 is provided with multiple boss 112 to support described card membrane 32.In the present embodiment two, the lower end in described upper pipe portion 322 is connected on described boss 112.
Please continue to refer to Fig. 7 and Fig. 8, described through hole 301 comprises the first pore 302 and the second pore 303.Described first pore 302 runs through and is opened in described upper pipe portion 322.Described second pore 303 runs through and is opened in described down leg 321.Described first pore 302 is communicated with the second pore 303 and arranges.As long as the shape of described first pore 302, size can realize impurity and nucleic acid circulates.In the present embodiment, described first pore 302 is offered as doline so that liquid-flow.Described first pore 302 also can be set to have the cross-sectional area being greater than described second pore 303.Described second pore 303 can be set to described filtering membrane 22 to have identical cross-sectional area and shape of cross section with this filtering membrane 22 accommodating.
Illustrate below in the present embodiment two, described card membrane 32 is to the restriction of described filtering membrane 22.The lower end of described second pore 303 can be set to and described filtering membrane 22 contacts, abut or interval this filtering membrane 22 to be limited in the bottom 3011 in described tube chamber 11.As preferably, described filtering membrane 22 is contained in described second pore 303.Described filtering membrane 22 can have the cross-sectional area identical with described second pore 303 and shape of cross section.Described second pore 303 be set to cylindrical hole.The diameter of the circular cross section of this second pore 303 can be set to 1 millimeter to 4 millimeters.In the present embodiment, described second pore 303 has the cross-sectional area identical with described filtering membrane 22 and shape of cross section.
Refer to Fig. 8, preferably, the inside sidewalls of described down leg 321 is provided with the first stop portion 3211.This first stop portion 3211 exits for stopping in through hole 301 described in described filtering membrane 22.In the present embodiment, described first stop portion 3211 is set to the first annular fin or at least two projections.When stressed, elastic deformation is there is and can cross described first stop portion 3211 effortlessly to make described filtering membrane 22 when stressed in this first stop portion 3211 with at least one in described filtering membrane 22.Described filtering membrane 22 crosses described first stop portion 3211 to exit from described through hole 301 when being subject to the external force towards the top of described collection post 10, to realize dismounting.Described filtering membrane 22 moves to when being subject to the external force towards the bottom of described collection post 10 and is limited in described through hole 301, to realize installing.Certainly, described second pore 303 also can be set to abut against with described filtering membrane 22 to prevent this filtering membrane 22 from exiting.
See also Fig. 9, preferably, the bottom 3031 in the second pore 303 of described down leg 321 is provided with the first ring-shaped step or the first boss (not shown).In the present embodiment two, the bottom 3031 in the second pore 303 of described down leg 321 is provided with the first right-angled intersection barrier rib 3212.This first right-angled intersection barrier rib 3212 prevents described filtering membrane 22 from bottom 3031 landing.Preferably, this first right-angled intersection barrier rib 3212 is set to flush with described down leg 321.
The following describes the installation of embodiment two, removing relation: during installation, first can apply external force to filtering membrane 22, to make, this filtering membrane 22 is stressed crosses described first stop portion 3211.Described filtering membrane 22 can drop on the barrier rib 3212 of cross type.Now, described filtering membrane 22 is limited between the barrier rib 3212 of described first cross type and described first stop portion 3211, thus is arranged on by filtering membrane 22 in the second pore 303 in described card membrane 32.Then, more described card membrane 32 entirety is applied external force, cross the second stop portion 111 in described tube chamber 11 to make this card membrane 32.The down leg 321 of described card membrane 32 can drop on down on described boss 112 (or bottom of conical tube chamber 11).Now, described card membrane 32 is limited between described boss 112 and described second stop portion 111.When dismantling, external force can be applied by instrument or manually to filtering membrane 22, such that this filtering membrane 22 is stressed to be crossed the first stop portion 3211 and take out so that replacing filtering membrane 22 in described second pore 303.Then, then external force is applied to card membrane 32 make this card membrane 32 cross described second stop portion 111, thus take out described card membrane 32 so that change this card membrane 32.Certainly, also can first described card membrane 32 be arranged in described tube chamber 11, filtering membrane 22 is arranged in the second pore 303 of card membrane 32.During dismounting, also card membrane 32 entirety can be taken out in described tube chamber 11, and then filtering membrane 22 is taken out in described second pore 303.
It should be noted that, described filtering membrane 20 can be cylindrical or patty.Now, the diameter of the cross section of this filtering membrane 20 can be 1.5 millimeters to 10 millimeters.Preferably, in embodiment one, the diameter of the circular cross section of filtering membrane 20 is larger.In embodiment two, the diameter of the circular cross section of described filtering membrane 22 is less.Thus, the utility model can change the filtering membrane of multiple different size.
Two kinds of different embodiments of the membrane of card described in the utility model 30,32 can respectively by the different mould of design two kinds for the production of.And make the instrument for cutting out filter membrane according to corresponding size.The filter membrane obtaining different diameter is cut out with this instrument.Embodiment two and embodiment one have identical collection post 10.Thus, only need filter needs according to difference and change the filtering membrane 20,22 of corresponding size, and select corresponding card membrane 30,32 to coordinate, diversified application can be realized, raise the efficiency and versatility.
Illustrate the purification process principle to nucleic acid that the utility model adopts below: utilize glass fibre element film under given conditions can the character of specific adsorption nucleic acid, carry out purifying with the centrifugal column being embedded with glass fibre element film.Usually three not steps are divided into, the first step is lysate sample and makes the nucleic acid in sample be attached on glass fibre element film, second step uses certain solution to wash, remove the material of some non-specific adsorption (as albumen, cell debris etc.), 3rd step changes solution condition, is again discharged in solution by the nucleic acid of absorption from glass fibre element film.
These are only the utility model preferred embodiment, and be not used in limitation protection domain of the present utility model, any amendment in the utility model spirit, equivalently to replace or improvement etc., be all encompassed in right of the present utility model.
Claims (10)
1. a purification devices, is characterized in that, comprising:
Collect post, this collection post offers tube chamber vertically;
Filtering membrane, this filtering membrane is arranged in described tube chamber;
Card membrane, this card membrane is arranged on to limit the activity of described filtering membrane in described tube chamber, and described card membrane has the through hole offered vertically.
2. purification devices according to claim 1, is characterized in that: described card membrane is ring washer; Described card membrane has the cross-sectional area identical with described filtering membrane and shape of cross section.
3. purification devices according to claim 1, is characterized in that: described card membrane comprises down leg and upper pipe portion, and described upper pipe portion is arranged in described down leg; Described upper pipe portion has the first pore, and described down leg has the second pore; Described first pore and the second pore head and the tail are connected to form described through hole; Described second pore has the caliber being less than described first pore; Described filtering membrane is contained in described second pore.
4. purification devices according to claim 3, is characterized in that: the bottom of described second pore is provided with the first right-angled intersection barrier rib, the first ring-shaped step or multiple first boss to prevent described filtering membrane landing.
5. purification devices according to claim 3, is characterized in that: the inside sidewalls of described second pore is projecting the first stop portion; Described first stop portion is set to the 3rd annular fin or at least two projections; Described filtering membrane is set to be limited between described first stop portion and the bottom of the second pore, and described filtering membrane be set to when stressed can taking out to be arranged in this second pore or in this second pore axially beyond described first stop portion along described collection post; And/or the inside sidewalls of described second pore is set to abut against with described filtering membrane, can moves in the second pore when described filtering membrane is arranged on stressed and take out to be arranged in this second pore or in this second pore.
6. purification devices according to claim 3, is characterized in that: the inside sidewalls of the tube chamber of described collection post is projecting the second stop portion; Described card membrane is set to be limited between described second stop portion and the bottom of described through hole, and described card membrane be set to when stressed can taking out to be arranged in this second pore or in this second pore axially beyond described second stop portion along described collection post; And/or the inside sidewalls of the tube chamber of described collection post is set to abut against with described card membrane, can moves in the second pore when described filtering membrane is arranged on stressed and take out to be arranged in this second pore or in this second pore.
7. purification devices according to claim 6, is characterized in that: described second stop portion is set to the second ring-type raised line, the second annular fin or the second annular boss; Elastic deformation is there is and crosses described second stop portion to make described card membrane can cross when stressed at least one in this second stop portion and described card membrane when stressed.
8. the purification devices according to claim 1 or 2 or 3, its feature: described filtering membrane and described card membrane are arranged in the tube chamber of described collection post removably.
9. the purification devices according to claim 1 or 2 or 3, is characterized in that: the tube chamber of described collection post is set to circular cylindrical cavity; The cross section of described card membrane is set to circle; Described filtering membrane is set to cylindrical.
10. the purification devices according to claim 1 or 2 or 3, it is characterized in that: the bottom of the tube chamber of described collection post is set to taper shape, or the bottom inside of this tube chamber is provided with the second right-angled intersection barrier rib, the second ring-shaped step or multiple second boss to prevent described filtering membrane or the landing of described card membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520881725.6U CN205170837U (en) | 2015-11-06 | 2015-11-06 | Purification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520881725.6U CN205170837U (en) | 2015-11-06 | 2015-11-06 | Purification device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205170837U true CN205170837U (en) | 2016-04-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520881725.6U Active CN205170837U (en) | 2015-11-06 | 2015-11-06 | Purification device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205170837U (en) |
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2015
- 2015-11-06 CN CN201520881725.6U patent/CN205170837U/en active Active
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