CN201016979Y - Non-contact conductive and fluorescence detecting apparatus of micro total analysis system - Google Patents
Non-contact conductive and fluorescence detecting apparatus of micro total analysis system Download PDFInfo
- Publication number
- CN201016979Y CN201016979Y CNU2007200479990U CN200720047999U CN201016979Y CN 201016979 Y CN201016979 Y CN 201016979Y CN U2007200479990 U CNU2007200479990 U CN U2007200479990U CN 200720047999 U CN200720047999 U CN 200720047999U CN 201016979 Y CN201016979 Y CN 201016979Y
- Authority
- CN
- China
- Prior art keywords
- chip
- detection
- fluorescence
- micro
- analysis system
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6482—Sample cells, cuvettes
Abstract
The utility model relates to an analyzing instrument, in particular to a detection device of a micro total analysis system for same time simultaneous contactless conductivity and fluorescence detection. Two contactless conductivity electrodes are arranged besides a separation channel of a chip. High frequency signals are input into one detection electrode. High frequency conductivity signals of solution in a micro-channel in a region between the two detection electrodes are detected on the other electrode. A light emitting diode is used for an exciting light source of the fluorescence detection. Incident lights of the light source are aligned with the region of the micro-channel between the two detection electrodes. The fluorescence signals are detected by a light detection element. The utility model has the advantages of realized same time simultaneous contactless conductivity and fluorescence detection and various obtained information.
Description
Technical field
The utility model relates to a kind of analytical instrument.
Background technology
Micro-total analysis system is with sampling, pre-service, adds reagent, reaction, separation, detection etc. and be integrated in a new technology of carrying out on the microchip, have analysis speed fast, contain much information, characteristics such as reagent consumption is few, pollute less, sample size is few, operation cost is low, the instrument volume is little.
In micro-total analysis system, detecting device is one of most important parts.The diversity of analytic sample requires detecting device to have versatility, and the minimum sample size of micro-fluidic chip needs highly sensitive detecting device to match.But the used detecting device of micro-total analysis system does not have a kind of detection technique can satisfy versatility and two requirements of high sensitivity simultaneously at present.It is the developing direction that using value is arranged that the detection technique of two or more different operating principles is made up.Consider the high sensitivity of fluoroscopic examination and the versatility that electricity is led detection, two kinds of technical combinations then can be realized versatility and high sensitivity preferably.
The micro-total analysis system fluoroscopic examination of having reported adopts gas laser or semiconductor laser to come induced fluorescence to detect more.It is big that gas laser has a volume, and power consumption is high, shortcomings such as cost height, and the excitation wavelength that semiconductor laser provides is limited, the fluorescent reagent that matches is less, thereby has limited its application.(Light-emitting diode, LED) induced fluorescence detects the detection that existing report is used for Capillary Electrophoresis with light emitting diode.
Existing non-contact electric conductivity detects and has the high shortcoming of insufficient sensitivity, and it and fluoroscopic examination are combined, and can bring into play the advantage of the two preferably.
Summary of the invention
The purpose of this utility model is the shortcoming that overcomes prior art, provide a kind of easy to use, better performances at the pick-up unit that carries out the micro-total analysis system of non-contact conductance and fluoroscopic examination with point simultaneously.
Micro-total analysis system is made up of chip, liquid stream drive system and detection system.The utility model in micro-total analysis system non-contact conductance is detected and fluoroscopic examination is carried out on same surveyed area simultaneously.Split tunnel side at chip is provided with two non-contact conductance electrodes, to one of them detecting electrode input high-frequency signal, and the high frequency conductance signal in the zone between two detecting electrodes of the solution in another electrode detection microchannel.The excitation source of fluoroscopic examination uses light emitting diode, and its incident light is aimed at the zone of microchannel between two non-contact conductance electrodes, and this regional solution is induced down at lumination of light emitting diode and produced fluorescence, detects fluorescence signal by testing light element.
Below the utility model is explained in detail.
First characteristics of the present utility model are that the same point that carries out non-contact conductance detection and fluoroscopic examination on micro-total analysis system detects.The capillary channel of chip detection position is other to be glued with two non-contact conductance electrodes, and the interior capillary channel of electrode and chip does not contact.The mode that the non-contact conductance detecting electrode is close to the outside of chip can be a Contiuum type or split type.The Contiuum type structure is the outside that detecting electrode is fixed on chip, fixed form has: plate metal (electroless plating) in the lateral electrode position of chip, print the outside that is coated with electrically-conducting paint, metal electrode is bonded in chip, also again on the chip in capillary channel both sides undercutting aperture, electrode is imbedded wherein.Split-type structural then is that detecting electrode independently is arranged on the flat board, and chip and electrode plate are stacked together, and chip is close to electrode plate.To one of them electrode input high-frequency signal, on another electrode, detect the high frequency conductance information in solution zone between detecting electrode in the capillary channel.Simultaneously, the incident light of light emitting diode is focused between two detecting electrodes on the zone corresponding to capillary channel, induce that solution produces fluorescence in this regional channel, on the direction that departs from the light emitting diode input path, detect fluorescence signal with testing light element.
The excitation source that second characteristic of the present utility model are fluoroscopic examinations is a light emitting diode.Light emitting diode has characteristics such as power consumption is low, output power stable, volume is little, the wavelength coverage span is big, the life-span is long as excitation source.
The testing light element of fluorescence detection device can be used photodiode, photomultiplier or photon counter.
The position relation of placing between light emitting diode and the testing light element has multiple.The one, between two electrodes of battery lead plate, establish a pore, light emitting diode is placed on the battery lead plate below, makes incident light see through pore and arrives the interior detection position of chip, and testing light element is placed on to depart to collect on the incident direction and detects fluorescence.The 2nd, light emitting diode is placed on a certain angle on the chip, make incident light focus on two interelectrode kapillary zones, testing light element is placed on to depart to receive on the incident direction and detects fluorescence.The 3rd, imbed optical fiber at the capillary channel end of chip, the light emitting diode incident light arrives two interelectrode detection positions by optical fiber and capillary channel, and the fluorescence of generation is collected by the testing light element that is placed on chip below or top and is detected.The 4th, imbed optical fiber at the capillary channel end of chip, the light emitting diode incident light arrives two interelectrode detection positions by optical fiber, and the optical fiber of the fluorescence of generation above or below chip is collected and is sent to testing light element.The 5th, light emitting diode is placed on the battery lead plate below, makes incident light see through the detection position of the interior two interelectrode capillary channels of pore arrival chip between detecting electrode, and the fluorescence of generation is collected by the optical fiber of chip top and is sent to testing light element.The 6th, adopt conllinear type structure, the light emitting diode incident light reflexes to object lens through beam splitter, arrive surveyed area behind the object lens focusing, the sample emitted fluorescence is collected through same object lens and arrive testing light element behind beam splitter, optical filter, lens, thereby realizes the detection to fluorescence.
Advantage of the present utility model and good effect are to be implemented in and carry out fluoroscopic examination and electricity simultaneously with point and lead detection, obtain multiple information.Volume is little, and is easy to use, and highly versatile is highly sensitive.
Description of drawings
Fig. 1 is the utility model complete machine structure synoptic diagram.
Fig. 2 is the electrode plate of the utility model detecting electrode and the structural representation of chip.
Fig. 3 is the utility model non-contact conductance and a kind of structural representation of fluoroscopic examination.
Fig. 4 is the another kind of structural representation of the utility model non-contact conductance and fluoroscopic examination.
Fig. 5 is the utility model non-contact conductance and another structural representation of fluoroscopic examination.
Among the figure, 1 is chip, and 2 and 3 is the non-contact conductance detecting electrode, 4 is non-contact conductivity measuring device, and 5 is fluorescence detection device, and 6 is split tunnel, 7 is light emitting diode, and 8 is testing light element, and 9 is the damping fluid waste liquid pool, 10 is buffer pool, and 11 is electrode plate, and 12 is sample cell, 13 is sample intake passage, and 14 is the sample waste liquid pool, and 15 is object lens, 16 is beam splitter, and 17 is optical filter, and 18 is lens.
Embodiment
Provide embodiment below by embodiment.
With reference to Fig. 1, the utility model is made up of chip (1), non-contact conductivity measuring device (4), fluorescence detection device (5) etc.Non-contact conductance electrode (2,3) is positioned at split tunnel (6) side position and is access to non-contact conductivity measuring device (4).The light path of the light emitting diode (7) of fluorescence detection device (5) and the light path of testing light element (8) are aimed at the zone that is positioned on the chip split tunnel (6) between two non-contact conductance electrodes (2,3).This regional solution is induced at incident light and is produced fluorescence down, receives fluorescence by testing light element (8), and fluorescence detection device (5) links to each other with testing light element (8) and detects fluorescence signal.
Can make absolute construction to chip, non-contact conductance electrode (2,3) is fixed on the battery lead plate (11).Chip (1) is placed on the electrode plate (11), makes the interior split tunnel of chip aim at two detecting electrodes (2,3), as shown in Figure 2.
Fluoroscopic examination can be adopted conllinear type structure, as shown in Figure 3.Light emitting diode (7) incident light reflexes to object lens (15) through beam splitter (16), object lens focus on two electrodes (2 with incident light, 3) surveyed area between, the sample emitted fluorescence becomes directional light after same object lens receive, being converted into after beam splitter (16), optical filter (17), lens (18) are received by testing light element (8) can be for the electric signal that detects.
Fluoroscopic examination also can be adopted structure as shown in Figure 4.Light emitting diode (7) incident light is departing from the incident direction of light through arriving the surveyed area between two detecting electrodes (2,3) behind optical filter (17), the lens (18), receives the sample emitted fluorescence with testing light element (8).
The fluoroscopic examination structure also can be as shown in Figure 4.Between two electrodes (2,3) of an above-mentioned electrode plate (11), establish a pore.Chip (1) is placed on the electrode plate (11), makes sense channel aim at two detecting electrodes (2,3) zone and pore.Light emitting diode (7) is placed on electrode plate (11) below, and incident light focuses on sense channel through optical filter (17), lens (18) by pore, induces solution generation fluorescence in the passage.Above chip, accept fluorescence and can obtain fluorescence signal with testing light element (8).
The non-contact conductance electrode also can be made the Contiuum type structure with chip.Particularly, with the local formula of plating electrode is plated on a side of chip; Or be imprinted on a side of chip with conductive material; Also can on chip, aperture be set, electrode is imbedded wherein in the capillary channel both sides.
The utility model is not limited to the foregoing description.
Claims (3)
1. micro-total analysis system non-contact electric conductivity and fluorescence detection device, comprise chip (1), non-contact conductance electrode (2,3), non-contact conductivity measuring device (4), fluorescence detection device (5), chip (1) is provided with split tunnel (6), non-contact conductance electrode (2,3) be positioned at split tunnel (6) side position and be access to non-contact conductivity measuring device (4), it is characterized in that, the light path of the excitation source (7) of fluorescence detection device (5) and the input path of testing light element (8) are aimed at the upward zone between two non-contact conductance electrodes (2,3) of chip split tunnel (6).
2. micro-total analysis system non-contact electric conductivity according to claim 1 and fluorescence detection device is characterized in that, the excitation source (7) of fluorescence detection device (5) is a light emitting diode.
3. micro-total analysis system non-contact electric conductivity according to claim 1 and fluorescence detection device is characterized in that, the testing light element (8) of fluorescence detection device (5) is photodiode, photomultiplier or photon counter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200479990U CN201016979Y (en) | 2007-01-30 | 2007-01-30 | Non-contact conductive and fluorescence detecting apparatus of micro total analysis system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200479990U CN201016979Y (en) | 2007-01-30 | 2007-01-30 | Non-contact conductive and fluorescence detecting apparatus of micro total analysis system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201016979Y true CN201016979Y (en) | 2008-02-06 |
Family
ID=39057474
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007200479990U Expired - Fee Related CN201016979Y (en) | 2007-01-30 | 2007-01-30 | Non-contact conductive and fluorescence detecting apparatus of micro total analysis system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201016979Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105044164A (en) * | 2015-07-14 | 2015-11-11 | 泸州品创科技有限公司 | Method for analyzing and detecting liquor characteristic before and after formation of liquor sediment |
| CN106198468A (en) * | 2016-06-30 | 2016-12-07 | 桂林理工大学 | Electrochemiluminescence combination detection method in the case of a kind of single drop |
| CN106769880A (en) * | 2016-12-14 | 2017-05-31 | 薛永富 | A kind of multi-pass potential detection method |
| CN107607475A (en) * | 2017-09-06 | 2018-01-19 | 京东方科技集团股份有限公司 | Micro-total analysis system and method |
-
2007
- 2007-01-30 CN CNU2007200479990U patent/CN201016979Y/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105044164A (en) * | 2015-07-14 | 2015-11-11 | 泸州品创科技有限公司 | Method for analyzing and detecting liquor characteristic before and after formation of liquor sediment |
| CN106198468A (en) * | 2016-06-30 | 2016-12-07 | 桂林理工大学 | Electrochemiluminescence combination detection method in the case of a kind of single drop |
| CN106198468B (en) * | 2016-06-30 | 2019-06-07 | 桂林理工大学 | Electrochemistry-fluorescence is combined detection method in the case of a kind of list drop |
| CN106769880A (en) * | 2016-12-14 | 2017-05-31 | 薛永富 | A kind of multi-pass potential detection method |
| CN107607475A (en) * | 2017-09-06 | 2018-01-19 | 京东方科技集团股份有限公司 | Micro-total analysis system and method |
| CN107607475B (en) * | 2017-09-06 | 2020-05-26 | 京东方科技集团股份有限公司 | Micro total analysis system and method |
| US11534758B2 (en) | 2017-09-06 | 2022-12-27 | Boe Technology Group Co., Ltd. | Micro total analysis system and method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20210072141A1 (en) | Radiation Carrier and Use Thereof in an Optical Sensor | |
| CN103529006B (en) | A kind of Portable fluorescence based on micro-fluidic chip detection device | |
| CN105300943B (en) | A kind of microscope integrated optical circuit system for drop fluorescence detection | |
| CN204462172U (en) | A kind of full-automatic high flux multicolor fluorescence immunochromatography chip analysis system | |
| Măriuţa et al. | Miniaturization of fluorescence sensing in optofluidic devices | |
| Wang et al. | Rechargeable battery-triggered electrochemiluminescence detection on microfluidic origami immunodevice based on two electrodes | |
| Yang et al. | A facile light-emitting-diode induced fluorescence detector coupled to an integrated microfluidic device for microchip electrophoresis | |
| CN201016979Y (en) | Non-contact conductive and fluorescence detecting apparatus of micro total analysis system | |
| CN103675053A (en) | Local electrochemical imaging test system based on surface plasma resonance | |
| CN101614655B (en) | Detection method of microfluidic chip based on photoacoustic technique and device thereof | |
| CN105319197A (en) | Liquid drop micro-fluidic chip based on microlens array | |
| CN104880450A (en) | Line focusing detection system for immunofluorescence reagent card | |
| CN103063646A (en) | Dual-purpose laser-induced fluorescence detecting system | |
| CN102353659B (en) | Detector for biochip fluorescent microspectrum and manufacture method thereof | |
| CN2921831Y (en) | Efficient capillary electrophoresis apparatus | |
| CN203310753U (en) | Multi-wavelength capillary electrophoresis fluorescence excitation device | |
| KR101712691B1 (en) | Bio-analysis using ball-ended incident and output optical fiber | |
| CN201016953Y (en) | Micro-flow control chip multi-channel electrochemical detecting device | |
| CN202216907U (en) | Fluorescence micro-spectrum detection device of biological chip | |
| CN105388131A (en) | Fluorescence detection instrument and system based on micro-fluidic chip | |
| CN201075088Y (en) | Multicenter atomic fluorescence optical spectrometer | |
| CN203502352U (en) | Small quantum-dot immune chromatography test strip detecting device | |
| KR20110116461A (en) | Diagnostic apparatus for immunoassay and immunoassay method using the same | |
| CN206930584U (en) | A kind of four-way capillary electrophoresis chip LED induced fluorescence detection systems | |
| KR101806763B1 (en) | Proactive portable algae detecting apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080206 Termination date: 20100130 |