CN201016953Y - Micro-flow control chip multi-channel electrochemical detecting device - Google Patents
Micro-flow control chip multi-channel electrochemical detecting device Download PDFInfo
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- CN201016953Y CN201016953Y CNU2007200480004U CN200720048000U CN201016953Y CN 201016953 Y CN201016953 Y CN 201016953Y CN U2007200480004 U CNU2007200480004 U CN U2007200480004U CN 200720048000 U CN200720048000 U CN 200720048000U CN 201016953 Y CN201016953 Y CN 201016953Y
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Abstract
The utility model relates to an analytical instrument, in particular to a voltammetry-contactless conductance same point multichannel electrochemical detection device of a micro-fluidic chip. Two contactless conductance detection electrodes are closely arranged on the external lateral of the micro-fluidic chip corresponding to a capillary channel for contactless conductance detection. A micro-channel between two detection electrodes relative to the contactless conductance is equipped with a working electrode used for voltammetry detection. The utility model is characterized in that same point multichannel electrochemical detection at the same time is realized. A plurality of detection information can be provided at the same time. The detection object range is expanded. In addition, the utility model has the advantages of small volume and convenient use.
Description
The utility model relates to a kind of analytical instrument, and the volt-ampere-non-contact conductance that is specifically related to a kind of micro-fluidic chip is with putting the multiple tracks electrochemical detection device.
Background technology
The micro-fluidic chip technology is with sampling, pre-service, adds reagent, reaction, separation, detection etc. and be integrated in a new technology of carrying out on the microchip, have analysis speed fast, contain much information, characteristics such as reagent consumption is few, pollute less, sample size is few, operation cost is low, the instrument volume is little.
Detection technique is one of core technology of micro-fluidic chip, directly influences detected object, detection sensitivity, the range of linearity and instrument cost.Present detection technique mainly contains uv absorption detection, laser-Induced Fluorescence Detection, electricity and leads that detection, volt-ampere detect etc.Wherein, it is more general that uv absorption detection and electricity are led detection, but sensitivity is lower; Laser-Induced Fluorescence Detection and volt-ampere detection sensitivity are higher, but determination object is few.
Various detection modes all have the relative merits of self, and coupling technique makes the relative merits of variety of way obtain complementation just again, the particularly realization that detects with point, not only for qualitative, quantitatively provide more reference information, and enlarged the detected object scope.Volt-ampere-non-contact conductance detects and then can utilize volt-ampere sensitivity that detects and the versatility of non-contact conductance, has electroactive and charged composition corresponding response is all arranged.
The two pick-up units of micro-fluidic chip appear in the newspapers, but are not to carry out on same point, and the detection information comparability of gained is not strong.
Summary of the invention
The purpose of this utility model is the shortcoming that overcomes prior art, and a kind of many pick-up units of volt-ampere-non-contact electric conductivity of making the micro-fluidic chip of simple, easy to use, better performances are provided, and realizes detecting simultaneously with point.
Micro-fluidic chip is made up of chip, liquid stream drive system and detection system.Micro-fluidic chip volt-ampere of the present utility model-non-contact conductance multiple tracks electrochemical detection device, two non-contact conductance detecting electrodes are close in the outside of chip along the position of capillary channel correspondence, capillary channel in detecting electrode and the chip does not contact, and makes non-contact conductance and detects.Volt-ampere detection scheme with point is: be provided with one on corresponding to the microchannel between non-contact conductance two detecting electrodes and be used for the working electrode that volt-ampere detects, the buffer pool of the styletable of chip is placed with the supporting contrast electrode of working electrode with to electrode.To non-contact conductance detecting electrode input high-frequency signal, detect the high frequency conductance signal in solution zone between two non-contact conductance detecting electrodes in the capillary channel, obtain non-contact conductance information.Simultaneously, have electroactive material redox reaction takes place on working electrode, the response current signal of generation is obtained the volt-ampere detection information of this component by the voltammertry device.Can carry out qualitative and quantitative analysis to measured object better in conjunction with two picture groups spectrum.
Below the utility model is explained in detail.
Used chip in micro-fluidic chip generally is composited by substrate and cover plate, and microflute is set on substrate, forms capillary channel with the compound back of cover plate.The material of substrate and cover plate can be used hard inorganic material, as glass, quartz, silicon etc.; Or macromolecule polymeric material.Substrate and cover plate compound, complex methods such as available heating, illumination.
The innovation of this experiment is the multiple tracks Electrochemical Detection of carrying out volt-ampere-non-contact conductance in micro-fluidic chip, and realizes detecting with some multiple tracks simultaneously, and multiple detection information can be provided simultaneously.
The mode that the quantity inspection of non-contact conductance detecting electrode is surveyed depends on the needs.To general detection, detecting electrode is two, and an electrode connects high-frequency signal source, and another electrode is accepted electrode as signal.The mode that the non-contact conductance detecting electrode is close to the outside of chip can be a Contiuum type or split type.The Contiuum type structure is the outside that detecting electrode is fixed on chip, fixed form has: plate metal (electroless plating) in the lateral electrode position of chip, print the outside that is coated with electrically-conducting paint, metal electrode is bonded in chip, also again on the chip in capillary channel both sides undercutting aperture, electrode is imbedded wherein.Split-type structural then is that detecting electrode independently is arranged on the flat board, and chip and electrode plate are stacked together, and chip is close to electrode plate.
The technical scheme that detects with the some volt-ampere is: scheme (), open an aperture on substrate, aperture is facing between non-contact conductance two detecting electrodes, and capillary channel goes directly, be used for placing working electrode, or when making substrate, working electrode be embedded in above-mentioned position.Scheme (two) before substrate and cover plate bonding, is covered with working electrode with methods such as plating or printings in inner flap, then with substrate and cover plate bonding.Contrast electrode and/or electrode placed the buffer pool of capillary end also can be with reference to the scheme of above-mentioned working electrode.It generally is three-electrode system that volt-ampere detects, and promptly forms by working electrode, contrast electrode with to electrode, also can be by working electrode and contrast electrode or two electrode systems that electrode is formed.Working electrode can be metal electrodes such as Pt, Pd, Au, Cu, and carbon electrodes such as prepared Chinese ink, glass carbon, carbon paste, carbon fiber can also be various modified electrodes.The shape of working electrode can be strip, little disk, column, tubulose etc.Contrast electrode can be mercurous chloride electrode, Ag/AgCl electrode etc., or plates AgCl get final product on the Ag silk, can also directly use the earth polar of separation high pressure.The method for making of little disk working electrode generally has Sealing Method, the method for forming, sedimentation, attachment method etc.Volt-ampere detects and can work under the permanent direct current or the mode of pulse.
Advantage of the present utility model and good effect are to have realized to carry out the multiple tracks Electrochemical Detection simultaneously with point, and multiple detection information can be provided simultaneously, have enlarged the detected object scope.In addition, its volume is very little, easy to use.
Description of drawings
Fig. 1 is the one-piece construction synoptic diagram of multiple tracks electrochemical detection device of the present utility model;
Fig. 2 is a multiple tracks electrochemical detection device ingredient of the present utility model (scheme one);
Fig. 3 is multiple tracks electrochemical detection device figure of the present utility model (scheme one);
Fig. 4 is the sectional drawing (scheme one) of multiple tracks electrochemical detection device of the present utility model;
Fig. 5 is a multiple tracks electrochemical detection device ingredient of the present utility model (scheme two);
Fig. 6 is multiple tracks electrochemical detection device figure of the present utility model (scheme two);
Fig. 7 is the sectional drawing (scheme two) of multiple tracks electrochemical detection device of the present utility model.
Among each figure, 1 is chip, and 2,3 is the non-contact conductance electrode, and 4 is non-contact conductivity measuring device, 5 is the volt-ampere pick-up unit, and 6 is split tunnel, and 7 is working electrode, and 8 is contrast electrode, 9 is to electrode, and 10 for having the substrate of microchannel, and 11 is cover plate, and 12 is electrode plate.
Embodiment
Provide embodiment below.
With reference to Fig. 1, utility model multiple tracks electrochemical detection device is formed (5) by chip (1), non-contact conductance pick-up unit (4) and volt-ampere pick-up unit.Chip is formed by substrate (10) and cover plate (11) bonding, and microflute is set on substrate, forms capillary channel (6) with the compound back of cover plate; Non-contact conductance detecting electrode (2,3) is close in the outside of chip along the position of microchannel correspondence, the capillary channel in detecting electrode and the chip does not contact; Chip is gone up at split tunnel (6) and be provided with a working electrode (7) between two non-contact conductance electrode, supporting with it contrast electrode (8) and electrode (9) is placed in the buffer pool of chip styletable has been formed volt-ampere-non-contact conductance jointly with the many pick-up units of point.
Volt-ampere pick-up unit scheme one: the split tunnel (6) in chip (1) is upward located out an aperture between two non-contact conductance electrodes (2,3), with the working electrode (7) made from then on the aperture cover plate (11) or the substrate (10) that pass chip arrive split tunnel (with reference to Fig. 2,3,4), non-contact conductance detecting electrode (2,3) is close in the outside of chip (1) then.Or casting substrate (10) with high molecular polymer when, working electrode (7) is embedded in above-mentioned position, be bonded to chip (1) with cover plate (11) again.
Volt-ampere pick-up unit scheme two: before substrate (10) and cover plate (11) bonding, with methods such as plating or printings working electrode (7) is covered on the cover plate (11) again, with substrate (10) and cover plate (11) bonding (with reference to Fig. 5,6,7), non-contact conductance detecting electrode (2,3) is close in the outer survey of chip (1) then then.
The utility model is not limited to the foregoing description.
Claims (3)
1. micro-fluidic chip multiple tracks electrochemical detection device, comprise chip (1), non-contact conductance electrode (2,3), non-contact conductivity measuring device (4), voltammertry device (5), chip (1) is provided with split tunnel (6), non-contact conductance electrode (2,3) be positioned at split tunnel (6) side position and be access to non-contact conductivity measuring device (4), it is characterized in that, chip (1) is gone up in two non-contact conductance electrodes (2 at split tunnel (6), 3) be provided with a working electrode (7) between, working electrode (7), contrast electrode (8) and/or electrode (8) is access to voltammertry device (5).
2. micro-fluidic chip multiple tracks electrochemical detection device according to claim 1 is characterized in that, contrast electrode (8) and the split tunnel (6) that electrode (8) is located at chip (1) gone up or place the terminal waste liquid pool of split tunnel (6).
3. micro-fluidic chip multiple tracks electrochemical detection device according to claim 1 is characterized in that, substrate or cover plate that contrast electrode (8) passes chip (1) reach on the split tunnel (6); Or be attached to the inner flap of chip (1) before substrate and the cover plate bonding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200480004U CN201016953Y (en) | 2007-01-30 | 2007-01-30 | Micro-flow control chip multi-channel electrochemical detecting device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200480004U CN201016953Y (en) | 2007-01-30 | 2007-01-30 | Micro-flow control chip multi-channel electrochemical detecting device |
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| Publication Number | Publication Date |
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| CN201016953Y true CN201016953Y (en) | 2008-02-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNU2007200480004U Expired - Fee Related CN201016953Y (en) | 2007-01-30 | 2007-01-30 | Micro-flow control chip multi-channel electrochemical detecting device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183669A (en) * | 2011-02-15 | 2011-09-14 | 中国科学院化学研究所 | Micro-fluidic chip for in-vivo on-line simultaneous detection of ascorbic acid and magnesium ion and preparation method thereof |
| CN105665042A (en) * | 2015-01-20 | 2016-06-15 | 天津农学院 | Micro-fluidic chip use method |
| CN110057877A (en) * | 2018-12-24 | 2019-07-26 | 中山大学 | The biosensor and its preparation method for being used to detect tumour cell of repeatable modification |
| TWI761196B (en) * | 2021-04-29 | 2022-04-11 | 國立中興大學 | Microfluidic biosensing chip integrating separate electrochemical electrodes |
-
2007
- 2007-01-30 CN CNU2007200480004U patent/CN201016953Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183669A (en) * | 2011-02-15 | 2011-09-14 | 中国科学院化学研究所 | Micro-fluidic chip for in-vivo on-line simultaneous detection of ascorbic acid and magnesium ion and preparation method thereof |
| CN102183669B (en) * | 2011-02-15 | 2013-07-17 | 中国科学院化学研究所 | Micro-fluidic chip for in-vivo on-line simultaneous detection of ascorbic acid and magnesium ion and preparation method thereof |
| CN105665042A (en) * | 2015-01-20 | 2016-06-15 | 天津农学院 | Micro-fluidic chip use method |
| CN105665042B (en) * | 2015-01-20 | 2017-06-27 | 天津农学院 | The application method of micro-fluidic chip |
| CN110057877A (en) * | 2018-12-24 | 2019-07-26 | 中山大学 | The biosensor and its preparation method for being used to detect tumour cell of repeatable modification |
| TWI761196B (en) * | 2021-04-29 | 2022-04-11 | 國立中興大學 | Microfluidic biosensing chip integrating separate electrochemical electrodes |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080206 Termination date: 20100130 |