CN1986828A - Fish scale collagen and its production process - Google Patents
Fish scale collagen and its production process Download PDFInfo
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- CN1986828A CN1986828A CN 200510132208 CN200510132208A CN1986828A CN 1986828 A CN1986828 A CN 1986828A CN 200510132208 CN200510132208 CN 200510132208 CN 200510132208 A CN200510132208 A CN 200510132208A CN 1986828 A CN1986828 A CN 1986828A
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Abstract
The present invention provides fish scale collagen and its production process, and is especially one fish scale collagen extracting enzyme process. The characteristic fish scale collagen extracting enzyme process includes the following steps: washing fish scale and heating treatment, crushing the fish scale mechanically, adding protein hydrolase for enzyme treatment in hot water, centrifuging the hydrolysis liquid, taking the supernatant, and drying the supernatant to obtain powder. The process can obtain fish scale collagen with high purity and low pollution in high yield.
Description
Technical field
The present invention relates to a kind of fish scale collagen and make the method for this fish scale collagen.More particularly, the present invention relates to a kind of method of utilizing enzyme process extraction fish scale collagen.
Background technology
Many collagen protein tissue extracts such as the collagen protein that uses as makeup, food, medical supplies, industry etc. in the past from Mammals such as ox, pig and chicken or poultry animal.In recent years, the mad cow disease of ox, the foot and mouth disease of pig and the domestic animals and fowls class eqpidemic disease problems such as bird flu of poultry take place in succession, and the security facing test of the collagen protein of domestic animals and fowls class adds that some human consumers use the livestock collagen protein that tendency hypersensitive is arranged.Therefore, use the land to produce the collagen protein that animal extracted, the secret worry on safe in utilization is then arranged.
Because the problems referred to above, the collagen protein of the fish that security in recent years is higher comes on the scene gradually, wherein with the collagen content of fish-skin and fish scale than horn of plenty, be commonly used to as the raw material of making the collagen protein product.But, the collagen protein that with the fish-skin is raw material has its special fish stink, and wherein lipid content height and gonorrhoea material are many, also can produce problems such as transmittance is low, therefore especially as the raw material moiety of cosmetics the time, its stink and gonorrhoea can limit the category that collagen of fish skin uses.
Opposite, fish scale contains 50% collagen protein and 50% hydroxyapatite (HAP) approximately, and the extraction of fish scale collagen is in the past sloughed ash with acidic aqueous solution again for sloughing fat with acetone and other organic solvent earlier, filters to obtain thick collagen protein.As Japanese Patent P2004-91418A number announcement, this case contriver utilizes the hydrochloric acid deliming, and add the water decomposition drying and obtain finished product, yet the time that this method must spend is long, the cost of manufacture height, and big to environmental injury, so be unfavorable for a large amount of making.Therefore, many contrivers are improved its operation, utilize enzyme process to decompose fish scale collagen, the method that is disclosed for Japanese Patent P2003-327599A number for example, after cleaning and utilize acid solution removal fat and treating the fish scale swelling, utilize three stage enzymes to handle again and extract fish scale collagen, the fish scale collagen that this method obtains is colourless, odorless and turbidity are low.But, because this method still uses salt acid dipping fish scale raw material, and must just can obtain fish scale collagen after enzyme extracts three days, thus its time-consuming consumption worker, and also can damage environment.
In view of this, developing a kind of high yield, low pollution and timesaving fish scale collagen manufacture method needs.When proposing economy and environmental protection dispute, so a kind of fish scale collagen that these characteristics are arranged can reduce the possibility of its dispute.
Summary of the invention
The object of the invention and advantage are described in down part, or can be by apparent in describing.
The object of the invention more particularly is a kind of method with enzyme process extraction fish scale collagen for a kind of method that extracts fish scale collagen is provided.
Another object of the present invention is provides a kind of by fish scale collagen that aforesaid method extracted.
According to the object of the invention, the fish scale collagen manufacture method with feature of the present invention comprises the following step:
(a) the fish scale raw material is cleaned after, with heat treated fish scale raw material;
(b) the fish scale raw material after the heat treated is handled with Mechanical Crushing;
(c) the fish scale raw material after step (b) is in small, broken bits adds 1% proteolytic enzyme, and carries out enzyme and handle under warm water;
(d) will be centrifugal through the hydrolyzed solution after step (c) enzyme is handled;
(e) take out through the centrifugal upper strata liquid later of step (d); And
(f) the upper strata liquid with step (e) gained is dried to powder.
In the manufacture method of fish scale collagen of the present invention, the type of heating in this step (a) is for to carry out heat treated to the fish scale raw material with 100 ℃ to 121 ℃.
In the manufacture method of fish scale collagen of the present invention, employed enzyme was the mixed enzyme that comprises a kind or 2 kinds proteolytic enzyme during this step (c) enzyme was handled.
In the manufacture method of fish scale collagen of the present invention, this proteolytic enzyme is a neutral protein matter lytic enzyme.
In the manufacture method of fish scale collagen of the present invention, the drying mode in this step (f) is for utilizing spraying drying.
Another purpose according to the present invention about the fish scale collagen that utilizes above-mentioned steps to make, is characterized by a kind of productive rate height, purity height, high infiltration, oligosaprobic fish scale collagen.
That is, the prepared fish scale collagen of a kind of manufacture method with fish scale collagen of the present invention, wherein this prepared collagen protein is that a kind of productive rate and purity all reach the micromolecular collagen more than 90%.
In the above-mentioned fish scale collagen extracting process, the fish scale raw material, for example but be not restricted to make a living fish scale or belt leather fish scale.
The step of heat treated fish scale raw material is selected in the fish scale collagen extracting process of the present invention, for example but do not limit any particular device, as water-bath, double walled kettle, modes such as sterilization still or pressure cooker are finished.
And the equipment of wherein selecting the tool physical damage to pulverize function with the step of physical disturbance processing fish scale raw material carries out mechanical destruction.The present invention does not limit any specific equipment.For example, equipment can be clarifixator, ultrasound, and crusher, fruit juice mixer or food conditioning device etc. are pulverized so that the fish scale raw material is destroyed.
But the invention provides the manufacture method of the fish scale collagen of mass production high purity, high-recovery, high permeability.The prepared fish scale collagen of the inventive method can be applicable to, but is not restricted to, aspects such as makeup, skin care products, nutritious prod, protective foods, heath food, normal food.The personnel that are familiar with this technology must understand, and the present invention is not limited to any use or purposes.
Other characteristics of the present invention will disclose in detail below and become apparent after specific embodiment is observed.
Description of drawings
Fig. 1 is for mentioning the method simplified schematic illustration of making fish scale collagen according to the present invention.
Embodiment
The present invention will describe special specific embodiment in detail.These specific embodiments are explained via invention and are provided, and are not to be intended in order to restriction the present invention.In scope of invention and spirit, the present invention exists and to tend to comprise these and other change and change.
The present invention is mentioned to be a kind of collagen protein and manufacture method thereof that obtains through the enzyme process extraction.
The present invention's one specific embodiment, Fig. 1 is the simplified schematic illustration of fish scale collagen manufacture method of the present invention.
Step a represents that fish scale raw material (10) is through cleaning (11) back with heating (12) processing fish scale raw material (10).Fish scale raw material used in the present invention (10) can make a living fish scale or belt leather fish scale.(for example but do not limit) for example water-bath, double walled kettle, sterilization still or pressure cooker etc. are used in heating (12).
The fish scale raw material (10) that step b represents to handle through heating (12) is with Mechanical Crushing (13).The machinery (13) that the present invention is used for this fragmentation step is (for example but be not restricted to) clarifixator for example, ultrasound, and crusher, fruit juice mixer or food conditioning device etc. are to destroy the fish scale raw material in small, broken bits.
Step c represents and will add 1% proteolytic enzyme through the fish scale raw material (10) after step b is in small, broken bits, and carries out enzyme and handle (14) under warm water.The present invention is a neutral protein matter lytic enzyme in order to the proteolytic enzyme of handling (14) as enzyme.
Steps d is represented will be through the hydrolyzed solution centrifugal (15) after step c enzyme is handled (14).
Step e represents to take out through steps d centrifugal (15) upper strata liquid later.
Step f represents upper strata liquid drying (16) powdered with step e gained.The inventive method is utilized (for example but be not restricted to) spraying drying for example, lyophilize, and warm air drying, modes such as cold air drying or drying under reduced pressure are dried to powder with upper strata liquid.A particular embodiment of the present invention uses spraying drying to carry out drying treatment.
Fish scale collagen of the present invention can be applicable to, but is not restricted to, makeup, skin care products, nutritious prod, protective foods, heath food, normal food etc.Must understand, the present invention does not limit any use or purposes.
Embodiment one (different heating time)
Getting 1000 gram fish scale raw materials cleans to remove impurity with clear water, be divided into five groups (each 200 gram) after waiting to clean, with 121 ℃ of heating 0,15,30,45 and 60 minute, to add the water of 400 grams through heating fish scale raw material later, (kinematica ) is broken into little thin piece with clarifixator, centrifugal (room temperature, 8000rpm, 30 minutes) get upper strata liquid, utilize spraying drying to be dried to upper strata liquid Powdered again.The fish scale collagen productive rate of embodiment one as shown in Table 1.In the table one, the ratio (%) of the product that " productive rate (%) " obtains through above-mentioned steps extraction for raw material, " thick collagen protein (%) " be for comprising the protein content (%) of collagen protein in the product, below identical.
Table one: the productive rate of the fish scale collagen of different heating time
| Heat-up time (minute) | Productive rate (%) | Thick collagen protein (%) |
| 0 | 6 | 0.7 |
| 15 | 53 | 2.5 |
| 30 | 42 | 3.7 |
| 45 | 46 | 3.8 |
| 60 | 63 | 4.0 |
Embodiment two (different heating temperature)
Getting 400 gram fish scale raw materials cleans to remove impurity with clear water, be divided into two groups (each 200 gram) after waiting to clean, heated 15 minutes with 100 ℃ and 121 ℃ respectively, heating fish scale raw material later is broken into little thin piece with clarifixator (kinematica ), the water and the 1%Protease N (PN that in the fish scale raw material after fragmentation, add 400 grams, available from sigma) act on 2 hours down in 50 ℃, after waiting to act on 2 hours immediately with whizzer (room temperature, 8000rpm, 30 minutes) centrifuging and taking upper strata liquid, utilize spray-dryer to be dried to upper strata liquid Powdered.The fish scale collagen purity of embodiment two and productive rate are as shown in Table 2.In the table two, " moisture (%) " is the moisture content (%) in the product, and " collagen protein purity (%) " is the content (%) of collagen protein in the thick collagen protein, below identical.
Table two: the productive rate of the fish scale collagen of different heating temperature and purity
| Heating temperature (℃) | Productive rate (%) | Moisture (%) | Thick collagen protein (%) | Collagen protein purity (%) |
| 100 | 85 | 92.9 | 6.3 | 88 |
| 121 | 94 | 91.9 | 7.2 | 89 |
Embodiment three (same enzyme is at different hydrolysis temperatures)
Getting 800 gram fish scale raw materials cleans to remove impurity with clear water, be divided into four groups (each 200 gram) after waiting to clean, with 121 ℃ of heating 15 minutes, heating fish scale raw material later is broken into little thin piece with clarifixator (Kinematica ), the water and the 1%Protease N (PN that in the fish scale raw material after fragmentation, add 400 grams, available from sigma) act on 2 hours down respectively at 35 ℃, 50 ℃, 60 ℃ and 75 ℃, after waiting to act on 2 hours immediately with whizzer (room temperature, 8000rpm, 30 minutes) centrifuging and taking upper strata liquid, utilize spray-dryer to be dried to upper strata liquid Powdered.The fish scale collagen purity of embodiment three and productive rate are as shown in Table 3.
Table three: the fish scale collagen productive rate and the purity of different hydrolysis temperatures
| Hydrolysis temperature (℃) | Productive rate (%) | Moisture (%) | Thick collagen protein (%) | Collagen protein purity (%) |
| 35 | 85 | 92.4 | 6.4 | 85 |
| 50 | 93 | 92.4 | 6.6 | 87 |
| 60 | 62 | 93.1 | 5.9 | 85 |
| 75 | 78 | 88.0 | 10.7 | 89 |
Embodiment four (different enzymes and different hydrolysis time)
Getting 1200 gram fish scale raw materials cleans to remove impurity with clear water, be divided into six groups (each 200 gram) after waiting to clean, with 121 ℃ of heating 15 minutes, heating fish scale raw material later is broken into little thin piece with clarifixator (kinematica ), the water and the 1%Protease N (PN that in the fish scale raw material after fragmentation, add 400 grams, available from sigma) under 50 ℃, act on 0.5,1,1.5,2,2.5 and 3.0 hour respectively, after waiting to act on immediately with whizzer (room temperature, 8000rpm, 30 minutes) centrifuging and taking upper strata liquid, utilize spray-dryer to be dried to upper strata liquid Powdered.
Getting 1200 gram fish scale raw materials cleans to remove impurity with clear water, be divided into six groups (each 200 gram) after waiting to clean, with 121 ℃ of heating 15 minutes, heating fish scale raw material later is broken into little thin piece with clarifixator (Kinematica ), the water and the 1%Protamex (Bacillus that in the fish scale raw material after fragmentation, add 400 grams, bacillus, available from sigma) under 50 ℃, act on 0.5 respectively, 1,1.5,2,2.5 and 3 hours, after waiting to act on immediately with whizzer (room temperature, 8000rpm, 30 minutes) centrifuging and taking upper strata liquid, utilize spray-dryer to be dried to upper strata liquid Powdered.
Getting 1200 gram fish scale raw materials cleans to remove impurity with clear water, be divided into six groups (each 200 gram) after waiting to clean, add 400 gram water respectively with 121 ℃ of heating 15 minutes, heating fish scale raw material later is broken into little thin piece with clarifixator (kinematica ), in the fish scale raw material after fragmentation, add 400 gram water and 1%Flavourzyme (Aspergillusoryzae, aspergillus oryzae, available from sigma) under 50 ℃, act on 0.5 respectively, 1,1.5,2,2.5 and 3 hours, after waiting to act on immediately with whizzer (room temperature, 8000rpm, 30 minutes) centrifuging and taking upper strata liquid, utilize spray-dryer to be dried to upper strata liquid Powdered.
Getting 200 gram fish scale raw materials cleans to remove impurity with clear water, wait to clean the back with 121 ℃ of heating 15 minutes, heating fish scale raw material later is broken into little thin piece with clarifixator (kinematica ), in the fish scale raw material after fragmentation, add 400 gram water under 50 ℃ with 1%Protease N (PN, available from sigma) effect 2 hours after again with 1%Flavourzyme (Aspergillus oryzae, available from sigma), act on 0.5 hour, after waiting to act on immediately with whizzer (room temperature, 8000rpm, 30 minutes) centrifuging and taking upper strata liquid, utilize spray-dryer to be dried to upper strata liquid Powdered.
The fish scale collagen productive rate of embodiment four as shown in Table 4, purity is as shown in Table 5.
Through the fish scale collagen molecular weight distribution of Protease N effect after 2.5 hours as shown in Table 7.In the table seven, " protein content (%) " is the Protein content in a certain specific molecular weight range in the thick collagen protein.
Table four: the fish scale collagen productive rate of different enzymes and different hydrolysis times
| Enzyme | Hydrolysis time (hour) | Productive rate (%) |
| Protease N | 0.5 | 79 |
| 1 | 86 | |
| 1.5 | 88 | |
| 2 | 93 | |
| 2.5 | 95 | |
| 3 | 93 | |
| Protamex | 0.5 | 56 |
| 1 | 56 | |
| 1.5 | 58 | |
| 2 | 53 | |
| 2.5 | 62 | |
| 3 | 58 | |
| Flavourzyme | 0.5 | 69 |
| 1 | 82 | |
| 1.5 | 81 | |
| 2 | 83 | |
| 2.5 | 71 | |
| 3 | 78 | |
| 2 hours+Flavourzyme of Protease N 0.5 hour | 2.5 | 93 |
Table five: the fish scale collagen purity of different enzymes and different hydrolysis times
| Enzyme | Hydrolysis time (hour) | Moisture (%) | Thick collagen protein (%) | Collagen protein purity (%) |
| Protease N | 0.5 | 92.9 | 6.6 | 92 |
| 1 | 93.1 | 6.3 | 91 | |
| 1.5 | 92.8 | 6.6 | 93 | |
| 2 | 92.2 | 7.2 | 93 | |
| 2.5 | 91.9 | 7.8 | 97 | |
| 3 | 90.8 | 8.5 | 93 | |
| Protamex | 0.5 | 94.5 | 5.0 | 90 |
| 1 | 94.4 | 4.9 | 88 | |
| 1.5 | 93.5 | 5.7 | 88 | |
| 2 | 94.1 | 5.1 | 86 | |
| 2.5 | 92.9 | 6.3 | 88 | |
| 3 | 93.3 | 5.9 | 88 | |
| Flavourzyme | 0.5 | 93.2 | 6.3 | 92 |
| 1 | 93.5 | 6.0 | 92 | |
| 1.5 | 92.8 | 6.5 | 91 | |
| 2 | 92.6 | 6.7 | 91 | |
| 2.5 | 93.7 | 5.7 | 90 | |
| 3 | 92.6 | 6.7 | 90 | |
| 2 hours+Flavourzyme of Protease N 0.5 hour | 2.5 | 92.7 | 6.6 | 91 |
Comparative example (different treatment condition)
A. overnight with the extraction of 0.5M acetic acid
Get 200 gram fish scale raw materials and clean to remove impurity with clear water, it is overnight with the extraction of 0.5M acetic acid to wait to clean the back, immediately with whizzer (room temperature, 8000rpm, 30 minutes) centrifuging and taking upper strata liquid, utilizes spray-dryer to be dried to upper strata liquid Powdered after waiting to act on.
B. the fish scale raw material does not heat direct hydrolysis not in small, broken bits
Getting 200 gram fish scale raw materials cleans to remove impurity with clear water, directly add 400 gram water after waiting to clean and go into 1%Protease N and act on 2 hours down in 50 ℃, after waiting to act on immediately with whizzer (room temperature, 8000rpm, 30 minutes) centrifuging and taking upper strata liquid, utilize spray-dryer to be dried to upper strata liquid Powdered.
C. the fish scale raw material does not heat directly in small, broken bits and hydrolysis
Getting 200 gram fish scale raw materials cleans to remove impurity with clear water, wait to clean the back and be broken into little thin piece with clarifixator (kinematica ), in the fish scale raw material after fragmentation, add 400 gram water and 1%Protease N and act on 2 hours down in 50 ℃, after waiting to act on immediately with whizzer (room temperature, 8000rpm, 30 minutes) centrifuging and taking upper strata liquid, utilize spray-dryer to be dried to upper strata liquid Powdered.
The fish scale collagen purity of comparative example and productive rate are as shown in Table 6.
Table six: the productive rate of comparative example and purity
| Productive rate (%) | Moisture (%) | Thick collagen protein (%) | Collagen protein purity (%) | |
| A | 3 | 99.2 | 0.03 | 4 |
| B | 39 | 95.7 | 2.6 | 59 |
| C | 54 | 95.1 | 4.5 | 73 |
Table seven: through the fish scale collagen molecular weight distribution of Protease N effect after 2.5 hours
| Molecular weight ranges | OD value (optical density value) | Protein content (%) |
| ＞5KD | 0.54 | 24.5 |
| 2-5KD | 0.399 | 18.1 |
| 1-2KD | 0.522 | 23.7 |
| ＜1KD | 0.392 | 17.8 |
| ＜5KD(＜2KD) | 59.6(41.5) | |
| Add up to | 1.853 | 84％ |
Experimental result shows, relatively productive rate can find out that fish scale collagen productive rate through 121 ℃ of heating is greater than the fish scale collagen productive rate (its productive rate is respectively 94% and 85%) through 100 ℃ of heating; The Protease N enzymic hydrolysis time, its fish scale collagen productive rate was between 93-95% between 2-3 hour.Otherwise the fish scale collagen productive rate with the extraction overnight of 0.5M acetic acid in the comparative example only is 3%, and not heating not in small, broken bits and not heating fish scale collagen productive rate in small, broken bits also only is 39% and 54%.It should be noted that fish scale collagen productive rate that fish scale collagen manufacture method that the present invention discloses produces up to more than 90%, is significantly higher than the fish scale collagen productive rate that comparative example is made.
Relatively purity can find out that the fish scale collagen purity via different heating temperature heat treated is respectively 88% and 89%, and the fish scale collagen purity of handling with different hydrolysis temperatures is between 85-89%; Fish scale collagen purity with 0.5 to 3 hour institute's output of 1%Flavourzyme effect is 90-92%, more between 91-97%, wherein the highest with the fish scale collagen purity of 0.5 to 3 hour institute's output of 1%Protease N effect with the fish scale collagen purity that acts on after 2.5 hours.Otherwise the fish scale collagen purity with the extraction overnight of 0.5M acetic acid in the comparative example only is 4%, and not heating not in small, broken bits and not heating fish scale collagen purity in small, broken bits only is 59% and 73%.It should be noted that fish scale collagen purity that fish scale collagen manufacture method that the present invention discloses produces up to more than 90%, and be significantly higher than the fish scale collagen purity that comparative example is made.
Table seven is for disclosing via the fish scale collagen molecular weight distribution situation of Protease N effect after 2.5 hours.The molecular weight of fish scale collagen of the present invention records by MALDI-TOF (laser desorption ionization flight time mass spectrum) technology.It should be noted that by the fish scale collagen molecular weight that can find out manufacture method of the present invention institute output in the table seven to account for 41.5%, and the following person of 5KD accounts for 59.6% the following person of 2KD.Hence one can see that, and the fish scale collagen molecule of manufacture method of the present invention institute output is little, also good to the penetration degree of skin comparatively speaking.
The present invention cleans from the fish scale raw material, heating, and Mechanical Crushing, enzymic hydrolysis is no more than 6 hours to centrifugal, compared with prior art, shortens fish scale collagen manufacturing time (shortening to 6 hours by 3 days) really.Significantly, the manufacture method of the present invention's announcement can produce the more fish scale collagen of volume in the identical time.
The above is only for preferred embodiment of the present invention, and so it is not in order to limiting scope of the present invention, and any personnel that are familiar with this technology without departing from the spirit and scope of the present invention, can do further improvement and variation on this basis.As above-mentioned explanation, about heating, Mechanical Crushing, enzyme is handled and centrifugal or the like can be done various modifications and variation, and can not depart from spirit of the present invention and scope.So the scope that claims were defined that protection scope of the present invention is worked as with the application is as the criterion.
Being simply described as follows of symbol in the accompanying drawing:
10～fish scale raw material
11～clean
12～heating
13～Mechanical Crushing
14～enzyme is handled
15～centrifugal
16～drying
Claims (8)
1. the manufacture method of a fish scale collagen, it comprises following step:
(a) the fish scale raw material is cleaned after, with heat treated fish scale raw material;
(b) the fish scale raw material after the heat treated is handled with Mechanical Crushing;
(c) the fish scale raw material after step (b) is in small, broken bits adds 1% proteolytic enzyme, and carries out enzyme and handle under warm water;
(d) will be centrifugal through the hydrolyzed solution after step (c) enzyme is handled;
(e) take out through the centrifugal upper strata liquid later of step (d); And
(f) the upper strata liquid with step (e) gained is dried to powder.
2. the manufacture method of fish scale collagen according to claim 1 is characterized in that, this fish scale raw material make a living fish scale or belt leather fish scale.
3. the manufacture method of fish scale collagen according to claim 1 is characterized in that, the type of heating in this step (a) is for to carry out heat treated to the fish scale raw material with 100 ℃ to 121 ℃.
4. the manufacture method of fish scale collagen according to claim 1 is characterized in that, the Mechanical Crushing in this step (b) is treated to utilizes clarifixator that the fish scale raw material is in small, broken bits.
5. the manufacture method of fish scale collagen according to claim 1 is characterized in that, employed enzyme was the mixed enzyme that comprises a kind or 2 kinds proteolytic enzyme during this step (c) enzyme was handled.
6. the manufacture method of fish scale collagen according to claim 5 is characterized in that, this proteolytic enzyme is a neutral protein matter lytic enzyme.
7. the manufacture method of fish scale collagen according to claim 1 is characterized in that, the drying mode in this step (f) is for utilizing spraying drying.
8. the prepared fish scale collagen of the manufacture method with the described fish scale collagen of claim 1 is characterized in that, this prepared collagen protein is that a kind of productive rate and purity all reach the micromolecular collagen more than 90%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009086743A1 (en) * | 2008-01-09 | 2009-07-16 | Body Organ Biomedical Corp. | A method for preparing a biomaterial |
| CN102106502A (en) * | 2011-02-01 | 2011-06-29 | 完美（中国）有限公司 | Health-care food with functions of improving skin moisture and reinforcing immunity |
| CN103333220A (en) * | 2013-07-22 | 2013-10-02 | 东北林业大学 | Degreasing and de-ashing method for extracting collagen from fish scales |
| CN103536003A (en) * | 2013-10-13 | 2014-01-29 | 苏州圣韵工艺科技有限公司 | Production method of fish scale garment hanging decoration |
| CN110643666A (en) * | 2019-11-18 | 2020-01-03 | 汕尾市维明生物科技股份有限公司 | Fish scale collagen peptide and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1683550B (en) * | 2005-03-08 | 2010-12-01 | 国家海洋局第一海洋研究所 | Collagen and its preparing process |
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2005
- 2005-12-22 CN CNB200510132208XA patent/CN100485043C/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009086743A1 (en) * | 2008-01-09 | 2009-07-16 | Body Organ Biomedical Corp. | A method for preparing a biomaterial |
| CN101480503B (en) * | 2008-01-09 | 2012-10-17 | 柏登生医股份有限公司 | Method for formulating biological material |
| CN102106502A (en) * | 2011-02-01 | 2011-06-29 | 完美（中国）有限公司 | Health-care food with functions of improving skin moisture and reinforcing immunity |
| CN102106502B (en) * | 2011-02-01 | 2013-10-16 | 完美（中国）有限公司 | Health-care food with functions of improving skin moisture and reinforcing immunity |
| CN103333220A (en) * | 2013-07-22 | 2013-10-02 | 东北林业大学 | Degreasing and de-ashing method for extracting collagen from fish scales |
| CN103333220B (en) * | 2013-07-22 | 2014-11-26 | 东北林业大学 | Degreasing and de-ashing method for extracting collagen from fish scales |
| CN103536003A (en) * | 2013-10-13 | 2014-01-29 | 苏州圣韵工艺科技有限公司 | Production method of fish scale garment hanging decoration |
| CN110643666A (en) * | 2019-11-18 | 2020-01-03 | 汕尾市维明生物科技股份有限公司 | Fish scale collagen peptide and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN100485043C (en) | 2009-05-06 |
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