CN1975367A - Tissue paraffin section de-waxing method - Google Patents
Tissue paraffin section de-waxing method Download PDFInfo
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- CN1975367A CN1975367A CN 200610129808 CN200610129808A CN1975367A CN 1975367 A CN1975367 A CN 1975367A CN 200610129808 CN200610129808 CN 200610129808 CN 200610129808 A CN200610129808 A CN 200610129808A CN 1975367 A CN1975367 A CN 1975367A
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- dewaxing
- paraffin section
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- tissue paraffin
- waxing method
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Abstract
This invention disclosed a de-waxing method used for paraffin-cut section. De-waxing solution was prepared by mixing distilled water and Triton X-100 by volume ratio of 99:1. It can be used by the following procedure: place the slice into the first de-wax solution and dip for 10-2 minutes at 60-70DEG C; take it out and place it to the second de-wax solution and repeat the above operation; wash it by water. The solution Triton X-100 used in this invention was safe, cost save, environmental friendly and it's easy to handle.
Description
[technical field]
The present invention relates to pathology histotomy technical field, particularly a kind of tissue paraffin section de-waxing method with simple and easy, environmental protection, the cheap characteristics of expense.
[background technology]
Tissue paraffin section de is the most frequently used a kind of microtomy in qualitative analyses such as tissues observed form and SABC, is widely used in clinical pathology diagnosis and experimental study.At first to remove the paraffin of paraffin section for tissues observed, promptly take off paraffin.The conventional process for dewaxing of tissue paraffin section de is to use organic solvent, and for example using dimethylbenzene is dewaxing agent commonly used.In this process for dewaxing, not only operate loaded down with trivial detailsly, need a plurality of steps and xylene pollution environment, harmful.Yet for improving this situation, up to the present, the process for dewaxing of improvement is still many to be dewaxing agent with the organic solvent, or uses the dewaxing of liquid detergent solution, but human body and environment are also still had harmful effect.
[summary of the invention]
Purpose of the present invention is intended to for overcoming the deficiencies in the prior art, and a kind of tissue paraffin section de-waxing method is provided, and this method is simple to operate, environmental protection, safety.
The present invention discloses a kind of tissue paraffin section de-waxing method for addressing the above problem.It is characterized in that said dewaxing step is: the first cylinder dewaxing liquid is put in the paraffin organization section, and constant temperature soaked 10-20 minute under 60-70 ℃ of temperature; Directly put into the second cylinder dewaxing liquid again with the same terms repetition previous action after taking out section; The water flushing is promptly finished then; Said dewaxing liquid is the poly-ethanol octyl phenyl ether aqueous solution of 1%-1.5% by volume that contains of water preparation.
The invention also discloses a kind of tissue paraffin section de and need the multiple tissue paraffin section de-waxing method of antigen hot repair as SABC.It is characterized in that said dewaxing step is: earlier dewaxing liquid is preheating to 95 ℃ to 100 ℃, 5-10 minute, again histotomy is put into dewaxing liquid and soaked 10-20 minute, promptly finish with the tap water flushing then; Said dewaxing liquid is the poly-ethanol octyl phenyl ethereal solution of 1%-1.5% by volume that contains with the preparation of antigen retrieval liquid.
The invention has the beneficial effects as follows: poly-ethanol octyl phenyl ether (Triton X-100) has no adverse effects to human body, and operating process is simple, does not produce environmental contaminants, and expense is cheap, and dewaxing effect is clear and definite.When needing the antigen hot repair multiple as SABC with tissue paraffin section de, can directly repair liquid and dewax simultaneously and antigen retrieval with dewaxing, do not need to dewax separately.Overcome that the operation of conventional lost-wax process is loaded down with trivial details, contaminated environment, healthy problem is harmful to health.Reusable 2-3 time of dewaxing liquid of the present invention in addition.
[embodiment]
Tissue paraffin section de-waxing method of the present invention the steps include: tissue paraffin section de is put into the first cylinder dewaxing liquid, and constant temperature soaked 10-15 minute under 60-65 ℃ of temperature; Directly put into the second cylinder dewaxing liquid again after taking out section, constant temperature soaked 10-15 minute again under 60-65 ℃ of temperature; Promptly finish with the tap water flushing then.
When needing the antigen hot repair multiple with tissue paraffin section de as SABC, the dewaxing liquid that can directly be used for antigen retrieval dewaxes and antigen retrieval simultaneously, does not need to dewax separately.When dewaxing is repaired, the dewaxing liquid that will be used for antigen retrieval put into the antigen retrieval box be preheating to temperature required general more than 95 ℃, 5-10 minute, again histotomy is put into the antigen retrieval box and dewaxes and antigen retrieval, the time is with general antigen retrieval liquid.Only get final product with a cylinder this moment.
Wherein:
1) preparation of dewaxing liquid
With distilled water and Triton X-100 (chemical name: poly-ethanol octyl phenyl ether (analyzing pure)) mix at 99: 1, fully stir with volume ratio.
2) dewaxing liquid that is used for antigen retrieval is prepared
With antigen retrieval liquid, mix at 99: 1 with volume ratio with Triton X-100 as citrate salt damping fluid or EDTA solution, fully stir.
Embodiment:
With the dewaxing liquid for preparing as stated above, pour two glass decorating cylinders into, and put into the water-bath preheating.Tissue paraffin section de is soaked in wherein in the cylinder, soaks 10 minutes in 60 ℃ of constant temperature.After histotomy directly taken out, put into another cylinder again, soaked 10 minutes in 60 ℃ of constant temperature.Then histotomy is taken out from solution, wash with tap water.Can carry out haematoxylin-Yihong dyeing according to a conventional method again, conventional dehydration, steps such as neutral gum sealing.
Dewaxing liquid is made into the dewaxing liquid that is used for antigen retrieval, and with the multiple box reparation of antigen hot repair, the antigen hot repair behind the together general tissue paraffin section de-waxing of method is multiple, after histotomy takes out from solution, with tap water flushing, promptly feasible immunohistochemical staining, conventional dehydration, the neutral gum sealing.
The present invention takes double-blind study to read sheet, compares with conventional dimethylbenzene process for dewaxing, and the equal indifference of coloration result, dewaxing liquid is definite with the dewaxing liquid dewaxing effect that is used for antigen retrieval.
Claims (7)
1, a kind of tissue paraffin section de-waxing method is characterized in that said dewaxing step is: tissue paraffin section de is put into the first cylinder dewaxing liquid, and constant temperature soaked 10-20 minute under 60-70 ℃ of temperature; Directly put into the second cylinder dewaxing liquid again with the same terms repetition previous action after taking out section; The water flushing is promptly finished then; Said dewaxing liquid is the poly-ethanol octyl phenyl ether aqueous solution of 1%-1.5% by volume that contains of water preparation.
2, tissue paraffin section de-waxing method according to claim 1 is characterized in that said dewaxing liquid fully mixes with volume ratio with distilled water and poly-ethanol octyl phenyl ether at 99: 1 to be prepared from.
3, tissue paraffin section de-waxing method according to claim 1 and 2, the thermostat temperature that it is characterized in that said dewaxing liquid is 60-65 ℃.
4, tissue paraffin section de-waxing method according to claim 1 and 2, it is characterized in that said in dewaxing liquid soak time be 10-15 minute, and soak twice.
5, tissue paraffin section de-waxing method according to claim 1 and 2 is characterized in that said wash water is a tap water.
6, a kind of tissue paraffin section de-waxing method is characterized in that said dewaxing step is: earlier dewaxing liquid is preheating to 95 ℃ to 100 ℃, and 5-10 minute, again histotomy is put into dewaxing liquid and soaked 10-20 minute, promptly finish with the tap water flushing then; Said dewaxing liquid is the poly-ethanol octyl phenyl ethereal solution of 1%-1.5% by volume that contains with the preparation of antigen retrieval liquid.
7, tissue paraffin section de-waxing method according to claim 6 is characterized in that said antigen retrieval liquid is citrate salt damping fluid or EDTA solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101298085A CN100537750C (en) | 2006-12-01 | 2006-12-01 | Tissue paraffin section de-waxing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101298085A CN100537750C (en) | 2006-12-01 | 2006-12-01 | Tissue paraffin section de-waxing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1975367A true CN1975367A (en) | 2007-06-06 |
| CN100537750C CN100537750C (en) | 2009-09-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006101298085A Expired - Fee Related CN100537750C (en) | 2006-12-01 | 2006-12-01 | Tissue paraffin section de-waxing method |
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| CN (1) | CN100537750C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103940648A (en) * | 2014-04-04 | 2014-07-23 | 山西农业大学 | Preparation method for gill tissue paraffin section |
| CN105352775A (en) * | 2015-12-11 | 2016-02-24 | 武汉纺织大学 | Full automatic paraffin-embedded tissue section deparaffinating system |
| CN108398305A (en) * | 2018-04-28 | 2018-08-14 | 福州迈新生物技术开发有限公司 | A kind of paraffin section de-waxing device |
| CN109827821A (en) * | 2019-03-14 | 2019-05-31 | 武汉原谷生物科技有限责任公司 | A kind of non-dimethylbenzene dewaxing renovation agent and preparation method thereof for paraffin section |
| CN109975095A (en) * | 2019-02-22 | 2019-07-05 | 嘉兴雅康博医学检验所有限公司 | A kind of pretreatment liquid and pre-treating method of fluorescence in situ hybridization |
| CN113686634A (en) * | 2021-08-10 | 2021-11-23 | 杭州迪英加科技有限公司 | Dewaxing repair liquid beneficial to acquisition of paraffin section images |
-
2006
- 2006-12-01 CN CNB2006101298085A patent/CN100537750C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103940648A (en) * | 2014-04-04 | 2014-07-23 | 山西农业大学 | Preparation method for gill tissue paraffin section |
| CN103940648B (en) * | 2014-04-04 | 2016-02-24 | 山西农业大学 | The preparation method of fish gill tissue paraffin section |
| CN105352775A (en) * | 2015-12-11 | 2016-02-24 | 武汉纺织大学 | Full automatic paraffin-embedded tissue section deparaffinating system |
| CN105352775B (en) * | 2015-12-11 | 2018-04-10 | 武汉康录生物技术股份有限公司 | A kind of full-automatic paraffin tissue sections dewaxing systems |
| CN108398305A (en) * | 2018-04-28 | 2018-08-14 | 福州迈新生物技术开发有限公司 | A kind of paraffin section de-waxing device |
| CN109975095A (en) * | 2019-02-22 | 2019-07-05 | 嘉兴雅康博医学检验所有限公司 | A kind of pretreatment liquid and pre-treating method of fluorescence in situ hybridization |
| CN109827821A (en) * | 2019-03-14 | 2019-05-31 | 武汉原谷生物科技有限责任公司 | A kind of non-dimethylbenzene dewaxing renovation agent and preparation method thereof for paraffin section |
| CN109827821B (en) * | 2019-03-14 | 2021-11-09 | 武汉原谷生物科技有限责任公司 | Non-xylene dewaxing repairing agent for paraffin section and preparation method thereof |
| CN113686634A (en) * | 2021-08-10 | 2021-11-23 | 杭州迪英加科技有限公司 | Dewaxing repair liquid beneficial to acquisition of paraffin section images |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100537750C (en) | 2009-09-09 |
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Granted publication date: 20090909 Termination date: 20100104 |