CN1973631A - Biocontrol fungus for preventing and controlling plant mycosis and its prepn process - Google Patents
Biocontrol fungus for preventing and controlling plant mycosis and its prepn process Download PDFInfo
- Publication number
- CN1973631A CN1973631A CN200610088339.7A CN200610088339A CN1973631A CN 1973631 A CN1973631 A CN 1973631A CN 200610088339 A CN200610088339 A CN 200610088339A CN 1973631 A CN1973631 A CN 1973631A
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- CN
- China
- Prior art keywords
- bao man
- bauman
- bacterial strain
- lch0606
- acinetobacter calcoaceticus
- Prior art date
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Abstract
The present invention belongs to the field of biological pesticide technology, and includes four aspects: 1. separation and identification of Acinetobacter baumannii strain LCH0606; 2. preparation and application in preventing and controlling plant diseases of Acinetobacter baumannii liquid; 3. preparation and disease preventing application of Bauman bacterin; and 4. effective component analysis of Bauman bacterin. Acinetobacter baumannii liquid and Bauman bacterin have main effective component of Iturin A, and possess broad bactericidal spectrum, less germ resistance, no harm to plant, safety to animal, environment friendship and other advantages. In addition, Acinetobacter baumannii liquid and Bauman bacterin have certain germination promoting effect on rice seed.
Description
One, technical field
The invention belongs to biological pesticide technical field.
Two, background technology
The 21 century mankind are faced with many challenges, and one of subject matter is population and food problem.China's population accounts for 1/4th of world's total population, has only 6.8% of arable land, the world but plough, and this problem is especially outstanding in China.Therefore, improving grain yield is the strategic task that China concerns the people's livelihood.But according to investigations, and world's staple food crop and economic crops due to illness evil loss 20% in every year (Oerke et al, 1994.Crop Protection andCrop Production.Elsevier, Amsterdam, 808pp).China cereal crops are evil and lose 10-15% at least due to illness; if do not use bactericide fully; the cucumber loss in normal time is about 55%～75%, radish is 40%, sweet basket is 25%, apple is 30%, and beet is old ten thousand justice of 24%[, 1998. plant protection 24 (5): 33-36].This shows the critical role of bactericide in cereal crops and economic crops.In fact, if there is not the use of bactericide, crop in the world will have half can not plant under existence conditions.
The bactericide of using in the agricultural production at present mostly is synthetics.Though it is higher and use advantage easily that the chemosynthesis bactericide has preventive effect, have following shortcoming: 1. resistance problem is serious: particularly plant pathogenic fungi is fast to the speed that the resistance of systemic fungicide produces, resistance level is high.General application about 3 years, plant pathogenic fungi just can produce resistance to it.It is reported that existing 46 kinds of disease funguses have produced higher resistance (Gullino et al, 2000.Crop Protection 19:1-11) to benomyl.2. develop the expense costliness of new varieties bactericide: because more and more stricter to the requirement of bactericide, the development cost of new varieties bactericide is also increasing.Every bactericide of new varieties of researching and developing successfully need be studied more than 20000 compound in the U.S., spend about 0.8～100,000,000 dollar (Leroux, 1996, Pestic.Sci.47:191-197).3. environmental pollution: though the chemosynthesis bactericide is smaller to the harm that the people holds than the chemosynthesis insecticide, the chemosynthesis bactericide also has carcinogenesis, and to shortcomings such as wild and non-target organism are poisonous, Environmental compatibility is poor.Therefore, in the public nuisance-free agricultural chemicals of implementation and today of sustainable agriculture production, exploitation and development are difficult for the environmentally friendly biological bactericide of pollution that the causes generation resistance, less has become the inexorable trend that agricultural chemicals develops.
With respect to the chemosynthesis bactericide, the use of biological control and natural products has more tempting prospect, microbial bactericide particularly, microorganism is a biological group the hugest on the earth on the one hand, it not only has bio-diversity widely, but also can increase substantially the output of target substance by strain improvement, metabolic regulation, scale fermentation and other modern biotechnology, be easy to realize suitability for industrialized production; On the other hand, microbial bactericide (microorganism and the metabolite thereof of living) is safer than chemical pesticide, easily degraded, and synthetic cost is lower.
Endophyte (endophytes) is meant and is grown in a class peculiar microorganism that does not cause morbidity in the pin main body, the adaptive capacity to environment of host plant is regulated and strengthened to a large amount of endophytes that studies have shown that by producing secondary metabolites, as fixed nitrogen, drought resisting, (Chanway, 1996.Can.J.Bot.75:331-332 such as pest-resistant and disease-resistant; Wilsonet al., 1994.Mycologia 86:635-647).(Fisher et al. 1984.Bot.Helv.94:249-253) finds that when carrying out the endophyte biological activity assay 30% endophyte can both produce the material of antimycotic and/or bacterium to Fisher etc.; Especially (the Stierle et al. such as Stierle that work as the U.S., after 1993.Science 260:214-216) discovery two strains can produce the endogenetic fungus of taxol and analog thereof from the yew of the Pacific Ocean, make and from the endophyte secondary metabolite, separate novel antibacterial or more evidence-based [the Petrini etal.1992 of sterilization lead compound, Sydowia, 44:282-293].The present invention will report a strain endogenetic bacteria---the discovery and the prophylaxis effect thereof of Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain.
Bao Man acinetobacter calcoaceticus is a kind of commonplace a kind of endophytic bacterium that distributes, but very few to the biological function research of this bacterium.Report that this bacterium can degrading herbicide and oil pollution, have certain environmental-protection function (Roque et al., 2000.Scientia Agricola 57 (4): 723-728; Sarma et al., 2004.Can.J.Microbiol.50 (6): 405-414).This bacterium of discovered in recent years can be used for control (Tan Xiaoyan etc., 2006, the microorganism journal 46 (2): 292-296) of citrus ulcer.This invention is separated to the antifungal activity that a strain Bao Man acinetobacter calcoaceticus bacterial strain has wide spectrum from the camphor tree stem.These results of study show that Bao Man acinetobacter calcoaceticus has diversity and wide biological activity in the abundant kind, are a kind of biopesticides that potentiality to be exploited is arranged very much, are used for the control of plant disease.
Three, summary of the invention
Purpose of the present invention is intended to seek the new source of biopesticide, but utilizes the endophyte of plant resource and develop and a kind ofly can be used for the biocontrol microorganisms bacterial strain that the wide spectrum of fungal diseases of plants control, efficient industrialization are produced.Screening, the authentication method of Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain and this bacterial strain are provided; This bacterial strain product Bauman bacteria solution and Bao Man rhzomorph and preparation technology thereof are provided; The using method that Bauman bacteria solution and Bao Man rhzomorph controlling plant diseases is provided and promotes the rice paddy seed rudiment.
Concrete technical scheme of the present invention is as follows:
The bacterium that the present invention adopts is a Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain (Acinetobacterbaumannii LCH0606) (being designated hereinafter simply as the LCH0606 bacterial strain), be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preservation date is on July 6th, 2006, and preserving number is CGMCCNo.1752.
Production bacterial strain LCH0606 bacterial strain of the present invention divides the camphor tree stem from area, the Purple Mountain, China Jiangsu Province, microexamination, staining reaction, the experiment of conventional physio-biochemical characteristics and 16s rDNA sequence analysis through bacterium colony and form, this identification of strains is a new bacterial strain (LCH0606) of Bao Man acinetobacter calcoaceticus (A.baumannii), has following feature: 1. circular, smooth, the central protrusion in edge of bacterium colony rule, opaque canescence, the bacterium colony drying; 2. thalline is rod-short or club shape, Gram-negative, no gemma and flagellum; 3. biochemical reactions shows as: catalase test, arginine dihydrolase test, malonate utilization test, nitrate and nitrite reduction test are all positive; Oxidase test, gelatin liquefaction test, urease test, phenylalanine deaminase are tested all negative; Glucose, lactose, galactose, mannose, wood sugar and rhamnose can be utilized, but sucrose, maltose, mannitol and fructose can not be utilized; 4. 16s rDNA The sequencing results is carried out maximum homology relatively in GenBank, finds that 16s rDNA sequencing result and acinetobacter 16s rDNA sequence homology reach 100%.
Biological prevention and control agent of the present invention is Bauman bacteria solution and Bao Man rhzomorph, they have significant preventive and therapeutic effect to multiple important fungal diseases of plants, as soybean anthracnose, Phytophthora capsici disease, graw mold of tomato, wheat scab and rice sheath blight disease etc., possessed the basic proterties of suitability for industrialized production simultaneously.
The present invention prepares according to conventional method.I.e. separation screening bacteriostatic activity bacterial strain LCH0606 from the camphor tree plant; Prepare biological bactericide by liquid fermentation and centrifugal treating---Bauman bacteria solution; Bauman bacteria solution is through macroporous resin adsorption, ethanol elution and concentrating under reduced pressure Processing of Preparation efficient biologic germicide---Bao Man rhzomorph; Bauman bacteria solution or Bao Man rhzomorph pass through the plate measuring to the inhibitory action of pathogenic bacteria such as soybean anthracnose, Phytophthora capsici disease, graw mold of tomato, wheat scab and rice sheath blight disease; The activity that Bauman bacteria solution or Bao Man rhzomorph are prevented and treated rice sheath blight disease experimental results show that by potted plant; Bauman bacteria solution or Bao Man rhzomorph carry out according to a conventional method to the facilitation of rice paddy seed rudiment; The mensuration of Bauman bacteria solution or Bao Man rhzomorph antimicrobial component is by silica gel column chromatography and Sephadex LH-20 chromatography gained repeatedly, and is accredited as lipopeptid class material IturinA2, A3, A6 through modern spectroscopic techniques such as NMR, HSQC, DEPT, LC/MS.
Advantage of the present invention and effect: the present invention has stable natural material source, and bacterial classification derives from the camphor tree stem, and screening technique is simple; Raw materials used potato and the sucrose of mainly containing in the fermentation process, cost is lower; The preparation of Bauman bacteria solution and Bao Man rhzomorph is simple, and the Bao Man rhzomorph obtains through macroporous resin treatment, and treating capacity is big, and is easy to operate, is fit to large-scale industrial production.Constant product quality all has good stable to light, heat, soda acid.This product low toxicity, to people and animals' nonhazardous, crops are not had poisoning, environmental protection, and the controlling plant diseases effect is remarkable.
Four, description of drawings:
Fig. 1: Bauman bacteria solution is to the inhibitory action of plant pathogenic fungi growth.The test bacterial classification is respectively: 1, C.parasitica; 2, G.glycines; 3, P.capsici; 4, F.oxysporum; 5, B.cinerea; 6, F.graminearum; 7, R.solani.A represents Bauman bacteria solution (concentration: 0.1ml/ml) among the figure, B represents Bao Man rhzomorph (concentration: 0.1mg/ml), C represents negative control, sterile water is handled, D represent 75% reach gram would rather Wettable Powder (concentration: 0.1mg/ml), E representative contain 15% triadimefon trazodone wettable powder (concentration: 1mg/ml), cultivation temperature: 30 ℃, observing time: 40h.
Fig. 2: the Bao Man rhzomorph is to the preventive and therapeutic effect of rice sheath blight disease.30 ℃ of vernalization of rice paddy seed are to the long 1cm of root, rhizoctonia silk bacterium cake in the nearly foundation inoculation diameter 6mm of portion, spray the 2ml different solutions simultaneously in inoculation position, 30 ℃ of heat and moisture preserving 24h, remove the bacterium cake then, continue to cultivate 24h, observe different disposal brown root or black root length, estimate control effect rice sheath blight disease.A is commercial bactericide Da Kening (positive control) among the figure, and concentration is 1mg/ml, and B is aqua sterilisa (negative control), and C is a Bauman bacteria solution, and D is the Bao Man rhzomorph, and concentration is 1mg/ml.
Five, embodiment
Be described in further detail the present invention with embodiment below, but content of the present invention is not limited thereto.
Embodiment one: the separation of LCH0606 bacterial strain, evaluation
Fresh camphor tree stem picks up from the Purple Mountain, Nanjing, is cut into the segment about 1cm after running water is cleaned and dried.Immerse 75% ethanol and 40% each 3min of formalin surface sterilization successively, use sterile water wash then 3 times, to remove the assorted bacterium of surface contamination, with sterile scissors stem is vertically cut again, tangent plane is put on the potato sucrose agar plate down, every dull and stereotyped 5 stem sections, totally 5 flat boards.30 ℃ of cultivations are by day appearance of the new bacterium colony of observation, totally 7 days, picking list bacterium colony, line purifying.Whether in addition, put into 2～3 stems that do not shred or blade compares in identical potato sucrose culture medium flat plate, it is thorough to detect surface sterilizing.Obtained strains adopts the glycerine preserving process standby in-80 ℃ of refrigerator preservations.
The 10 strain camphor tree stem endogenetic bacterias that are separated to are inoculated in the 15mL test tube that contains 4mL potato sucrose medium 37 ℃, 100rpm shaking table cultivation 48h respectively; Get the potato sucrose medium mixing that 1mL and 9mL dissolve after the culture fluid filtration sterilization, pour in the culture dish that diameter is 9cm, make the potato sucrose culture medium flat plate that contains zymotic fluid 10%, putting into diameter after the cooling is the fresh plant disease fungus bacterium cake of 7mm, bacterium faces down, and measures colony diameter behind the cultivation 40h in 30 ℃ of incubators, and replaces zymotic fluid to make blank with the medium of same volume, calculate the inhibiting rate of zymotic fluid, triplicate to the test conk.Wherein, inhibiting rate (%)=[(control group bacterium colony average diameter-sample sets bacterium colony average diameter)/(control group bacterium colony average diameter-original bacterium sheet diameter)] * 100%, colony diameter unit: centimetre (cm).
According to the inhibition activity of zymotic fluid to plant pathogenic fungi anthrax-bacilus, phytophthora, botrytis cinerea, sickle-like bacteria and rhizoctonia, screen a strain wide spectrum, efficient antifungal active bacterial strain, through form, dyeing, Physiology and biochemistry and 16S rDNA sequence analysis, be accredited as Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain.
Embodiment two: the preparation method of Bauman bacteria solution
Seed culture medium is the potato sucrose medium, and the single colony inoculation of the LCH0606 of picking activation is cultivated to 4mL potato sucrose medium (15mL test tube), 37 ℃, 100rpm shaking table cultivation 12h.Inoculation 1% seed liquor is in 400mL potato sucrose culture media shaking vase (1L), and 37 ℃, 100rpm shaking table are cultivated 48-60h, and gained bacterium liquid is Bauman bacteria solution behind 5000-6000rpm bactofugation body.
Embodiment three: the preparation method of Bao Man rhzomorph
Bauman bacteria solution described in the embodiment two is the Bao Man rhzomorph through macroreticular resin X-5 absorption, 80% ethanol elution, decompression rotary evaporation drying.The macroreticular resin consumption is 1% of a Bauman bacteria solution, and last sample and elution flow rate are 4mL/min.Bao Man rhzomorph yield is 568mg/L.
Embodiment four: bacteriostatic activity experiment in Bauman bacteria solution and the Bao Man rhzomorph ware
Behind the potato sucrose medium mixing that Bauman bacteria solution described in the 1mL embodiment two and 9mL have dissolved, pour in the culture dish that diameter is 9cm, make the culture plate that contains Bauman bacteria solution 10%, putting into diameter then is the various fresh plant disease fungus bacterium cakes of 7mm, bacterium faces down, and measures colony diameter behind the cultivation 40h in 30 ℃ of incubators, and replaces supernatant to make blank with the medium of same volume, calculate the inhibiting rate of supernatant, triplicate to various test conks.
Inhibiting rate (%)=[(control group bacterium colony average diameter-sample sets bacterium colony average diameter)/(control group bacterium colony average diameter-original bacterium sheet diameter)] * 100%, colony diameter unit: centimetre (cm).
Use the same method and measure the bacteriostasis of Bao Man rhzomorph described in the embodiment three, working concentration is 0.1mg/mL; The positive contrast of the triazolone of 1mg/mL (15% triadimefon wetting powder).The result shows that Bauman bacteria solution is suitable with the positive control triazolone to the inhibitory action of each test plant disease fungus growth with the Bao Man rhzomorph, and no significant difference (table 1, Fig. 1-2).
Table one Bauman bacteria solution and Bao Man rhzomorph are to the inhibitory action (%) of plant pathogenic fungi growth
| Sample | C.parasitica | P.capsici | B.cinerea | F.graminearum | R.solani |
| Bauman bacteria solution | 90.35 | 66.70 | 78.63 | 60.75 | 76.00 |
| The Bao Man rhzomorph | 89.10 | 55.51 | 60.04 | 52.96 | 70.18 |
| Triazolone | 87.53 | 42.85 | 57.11 | 60.04 | 88.58 |
Embodiment five: the analysis of active substance:
Bao Man rhzomorph 6.85g described in the embodiment three is through silica gel column chromatography, chloroform-methanol gradient elution, and obtained component carries out bacteriostatic experiment in the ware, merges active component according to antibacterial result, is evaporated to driedly, obtains the brown medicinal extract of 280mg.This medicinal extract with dissolve with methanol after with Sephadex LH-20 purifying repeatedly, obtain white powder active component 40mg.Through mass spectrum and magnetic resonance detection analysis, this antimicrobial compound is a lipopeptide compound, is respectively Iturin A2, A3 and A6.Mass spectrograph model VG-ZAB-HS, NMR model Bruker AM500FT-NMR, chromatographic silica gel is the 200-300 order.
Embodiment six: Bauman bacteria solution and the experiment of Bao Man rhzomorph control rice banded sclerotial blight disease
(1) experiment in the ware: 30 ℃ of vernalization of 50 rice paddy seeds are to the long 1cm of root, rhizoctonia silk bacterium cake in the nearly foundation inoculation diameter 6mm of portion, spray the 2ml different solutions simultaneously in inoculation position, 30 ℃ of heat and moisture preserving 24h, remove the bacterium cake then, continue to cultivate 24h, observe different disposal brown root or black root length, estimate control effect rice sheath blight disease.The result shows that Bauman bacteria solution or Bao Man rhzomorph (1mg/ml) are to the control efficiency of rice sheath blight disease and commercial bactericide Da Kening (positive control, concentration 1mg/ml) quite (Fig. 3).
(2) potted plant experiment: get the rice seedling of sprouting about the long 2cm of back root, at root inoculation silborne fungal diseases Rhizoctonia solani Kuhn, use the spray of Bao Man rhzomorph solution (0.1mg/mL) described in Bauman bacteria solution described in the embodiment two and the embodiment three rice seedling root then respectively, be treated to contrast with sterile water, every group of 50 seeds, 30C cultivates 72h " Invest, Then Investigate " record banded sclerotial blight evil and infects situation, according to (0 grade: disease-free symptom of 5 grades of criteria for classification; 1 grade: light grey scab, length is less than 0.5cm; 2 grades of diseases: brown scab, length 0.5cm-1cm; 3 ranks: the black scab, more than the length 1cm, root is injured seriously), calculate and respectively organize the average disease index of paddy rice, average disease index=∑ [(diseased plant numbers at different levels * relative state of an illness progression)]/total strain number of investigation.The result shows that Bauman bacteria solution has good preventive and therapeutic effect to rice sheath blight disease, concentration is that the Bao Man rhzomorph solution of 0.1mg/mL also has certain preventive and therapeutic effect (table two) to rice sheath blight disease, the preventive effect of Bauman bacteria solution is 82%, and the preventive effect of Bao Man rhzomorph (0.1mg/mL) is 48%.
The preventive effect that table two, Bauman bacteria solution and Bao Man rhzomorph infect Rhizoctonia solani Kuhn
| Sterile water | Bauman bacteria solution | The Bao Man rhzomorph | |
| Average disease index | 2.56 | 0.46 | 1.32 |
Embodiment seven: the influence that Bauman bacteria solution is sprouted rice paddy seed
Select healthy water seed rice seed, 37 ℃ of pre-vernalization 24h use 30 ℃ of seed soaking of the Bao Man rhzomorph solution (0.1mg/mL) described in Bauman bacteria solution described in the embodiment two and the embodiment three 12h then respectively, are blank with the sterile water seed soaking, every group of 50 seeds.Again seeds treated is placed culture dish, be covered with the wetting filter paper of sterile water at the bottom of the ware, 30 ℃ of cultivation 3d, it is long to detect each group processing seed germination rate, main root length, root approximate number and stem.The result shows that rudiment has no significant effect to rice paddy seed for Bauman bacteria solution and Bao Man rhzomorph, but root length, root approximate number and stem length are had certain facilitation (table three).
The influence that table three, Bauman bacteria solution and Bao Man rhzomorph are sprouted rice paddy seed
| Sterile water | Bauman bacteria solution | The Bao Man rhzomorph | |
| Germination rate (%) | 92.59 | 88.46 | 92.30 |
| Main root long (cm) | 4.12 | 4.55 | 5.28 |
| Root approximate number (root) | 28.0 | 29.7 | 37.8 |
| Stem long (cm) | 1.37 | 2.14 | 2.01 |
Claims (9)
1. microorganism formulation that suppresses the various plants pathomycete, it is characterized in that this biologic product is Bauman bacteria solution and Bao Man rhzomorph, producing bacterial strain was Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain (Acinetobacterbaumannii LCH0606), and this bacterial strain has been deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on July 6th, 2006.
2. according to the preparation method of the described Bao Man of claim 1 acinetobacter calcoaceticus LCH0606 bacterial strain, it is characterized in that from the camphor tree stem, being separated to endogenetic bacteria, concrete steps are: plant stem is cut into segment about 1cm, immerse 75% ethanol and 40% each 3min of formalin surface sterilization successively, use sterile water wash then 3 times, to remove the assorted bacterium of surface contamination, with sterile scissors stem is longitudinally cut again, tangent plane is put on the potato sucrose agar plate down, every dull and stereotyped 5 stem tuber sections, totally 5 flat boards, 30 ℃ of cultivations, by day appearance of the new bacterium colony of observation, totally 7 days, picking list bacterium colony, the line purifying, obtained strains obtains the plant pathogenic fungi anthrax-bacilus through antagonistic experiment, phytophthora, botrytis cinerea, sickle-like bacteria and rhizoctonia have the bacterial strain of remarkable inhibiting activity---Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain, and in-80 ℃ of refrigerator preservations.
3. the Bauman bacteria solution that Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain according to claim 1 is produced is characterized in that the mixed culture fermentation liquid of Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain, the aseptic supernatant of gained behind the centrifugal 30min of 5000-6000rmp.
4. the preparation method of the Bauman bacteria solution that Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain according to claim 3 is produced, it is characterized in that Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain is fermented, zymotechnique comprises the preparation and the 1L shake flask fermentation of seed liquor, being prepared as of seed liquor adds 4mL potato sucrose medium in the 15mL test tube, the inoculation back is in 37 ℃, the 100rpm shaking table is cultivated 12h, be inoculated in the potato sucrose medium of 400ml by 1% seed liquor then, 37 ℃, 100rpm shake flask fermentation 48h, zymotic fluid is behind the centrifugal 30min of 5000-6000rmp, and the aseptic supernatant of gained is Bauman bacteria solution.
5. the Bao Man rhzomorph that Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain according to claim 1 is produced is characterized in that the medicinal extract of Bauman bacteria solution gained after macroporous resin adsorption concentrates.
6. the preparation method of the Bao Man rhzomorph that Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain according to claim 5 is produced, it is characterized in that using macroporous resin adsorption to concentrate, the macroreticular resin particle size is 100-400, with the fermentating liquid volume ratio be 1: 100, flow velocity is 4mL/min; Eluting solvent is 80% ethanol, and flow velocity is 4mL/min, and eluent is evaporated to dried with Rotary Evaporators, be the Bao Man rhzomorph.
7. the antifungus active substance that Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain according to claim 1 is produced is characterized in that lipopeptid class material, through being accredited as Iturin A2, A3 and A6.
8. Bauman bacteria solution that Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain according to claim 1 is produced and Bao Man rhzomorph are in the application of control soybean anthracnose, Phytophthora capsici disease, graw mold of tomato, wheat scab and rice sheath blight disease plurality of plant diseases.
9. Bauman bacteria solution that Bao Man acinetobacter calcoaceticus according to claim 1 is produced and Bao Man rhzomorph promote that rice paddy seed is taken root, application in the rudiment.
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| CN101401588B (en) * | 2008-11-07 | 2011-05-25 | 江苏省农业科学院 | Bauman bacteria solution active coarse extractum Pyrimethanil compositional bactericide and uses thereof |
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| CN101230327B (en) * | 2008-03-03 | 2010-06-16 | 中国热带农业科学院环境与植物保护研究所 | Plant pathogenic fungi antagonistic bacteria capable of generating siderophore and uses thereof |
| CN101401588B (en) * | 2008-11-07 | 2011-05-25 | 江苏省农业科学院 | Bauman bacteria solution active coarse extractum Pyrimethanil compositional bactericide and uses thereof |
| CN101695306B (en) * | 2009-10-12 | 2011-10-19 | 江苏省农业科学院 | Compound bactericide of active crude extract of baumannii bacteria liquid and tebuconazole and application thereof |
| CN102876597A (en) * | 2011-12-06 | 2013-01-16 | 刘常宏 | Preparation and application of ribonucleic acid (RNA) polymerase mutant for highly yielding antifungal substance Iturin A |
| CN108148774A (en) * | 2017-11-20 | 2018-06-12 | 中国烟草中南农业试验站 | Acinetobacter calcoaceticus, screening technique and the application of antagonism tobacco black shank bacterium |
| CN108148774B (en) * | 2017-11-20 | 2021-05-28 | 中国烟草中南农业试验站 | Acinetobacter calcoaceticus for antagonizing phytophthora parasitica, screening method and application |
| CN108048351A (en) * | 2017-12-19 | 2018-05-18 | 华南农业大学 | One plant of acyl homoserine lactones degradation bacteria and its application in disease control |
| CN108048351B (en) * | 2017-12-19 | 2021-02-19 | 华南农业大学 | Acylhomoserine lactone degrading bacterium and application thereof in disease control |
| CN108865815A (en) * | 2018-09-10 | 2018-11-23 | 保定市稀成生物科技有限公司 | Portable mould efficiently divides pure stick |
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