CN1956786A - System and method for accurate measuring volume of liquid in a pipette - Google Patents
System and method for accurate measuring volume of liquid in a pipette Download PDFInfo
- Publication number
- CN1956786A CN1956786A CNA2004800351322A CN200480035132A CN1956786A CN 1956786 A CN1956786 A CN 1956786A CN A2004800351322 A CNA2004800351322 A CN A2004800351322A CN 200480035132 A CN200480035132 A CN 200480035132A CN 1956786 A CN1956786 A CN 1956786A
- Authority
- CN
- China
- Prior art keywords
- volume
- pipette
- liquid
- advanced
- correction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/148—Specific details about calibrations
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/02—Identification, exchange or storage of information
- B01L2300/025—Displaying results or values with integrated means
Abstract
A device and method are provided for correcting a requested volume of liquid to aspirate/dispense by a liquid handling pipette based on the requested volume and/or a physical condition at the pipette. The pipette includes a piston drive mechanism configured to contact a piston assembly and move a piston rod of the piston assembly within a tip holder thereby causing regulation of an amount of liquid in the tip holder. The method includes selecting a requested volume at a pipette, the requested volume representing the amount of liquid to regulate, calculating a correction volume using a volume characterization, wherein the volume characterization characterizes a difference in the amount of the liquid regulated in the tip holder as a function of the requested volume, and displaying the correction volume to a user of the pipette thereby regulating the requested volume of liquid in the tip holder. The volume characterization is determined using a calibration process.
Description
Invention field
The present invention relates generally to a kind of pipette that is used to draw and distribute the liquid of adjustable volume.More particularly, the present invention relates to a kind of liquid sampling pipette of measuring the liquid of precise volumes.
Background of invention
In medicine, gene and protein research, biological study, drug development laboratory and other biological technology application, utilize pipette in various laboratory operation rules, to come handle laboratory.Utilize pipette that the liquid of certain volume is sucked in the pipette.The liquid of described volume can be distributed into one or more dispensed volume then.Piston drive mechanism moves by making piston component, controls liquid and draws with specific volume and distribute.Pipette can manual type be operated, and wherein the user utilizes the speed and the volume of pressure-sensitive knob Artificial Control liquid assimilating or distribution.Or pipette can electronic mode be operated, wherein the absorption of Electric Machine Control liquid and/or distribution.In any mode, pipette all can have such as the electronic component that shows required volume aspirated.The user utilizes the optional majority kind of the display parameter that is installed on the pipette, comprises speed, volume, absorption number, allotment etc.The motion of piston rod is controlled by the thrust that piston driving assembly applies.In electric pipette, the motion of piston rod is generally controlled by the little processor that is positioned at the pipette shell.
No matter be in electronic or on-electric pipette, amount of liquid error all can occur by the pipette practical adjustments based on various phenomenons.An observe phenomena occurs in when requiring the small size sampling.Actual volume sampled is greater than volume required.For little sample volume, error is owing to capillarity causes.Otherwise for big required liquid volume, actual volume sampled is less than volume required.For big sample volume, error is because the fluid column weight of compressed liquid causes.In addition, the appearance of error is based on the current operating temperature of pipette than calibration temperature.For example, the heating of pipette occurs in after the prolongation manipulation of being implemented by the user.The heating of pipette causes regulating an element of the amount of liquid of drawing or distributing and expands, and produces the error of required volume aspirated thus.In cold pipette, component shrinkage.The appearance of other error is based on the different current atmospheric condition of pipette of atmospheric condition when calibrating.Atmospheric parameter when atmospheric temperature, pressure and/or the humidity when for example, operating pipette can be different from the pipette calibration.Therefore, what need is a kind of current physical state and/or current volume required bearing calibration of pipette, can handle at liquid whereby and accurately regulate required liquid volume in the pipette.What further need is that a kind of precision that improves pipette reduces the selling price of pipette simultaneously and makes the method for simplifying of making.
Summary of the invention
An example embodiment of the present invention relates to a kind of method of coming the required liquid volume in the actuated pipette of regulator solution by the current physical state of proofreading and correct pipette.This method includes, but is not limited to: select required volume on pipette, pipette comprises piston drive mechanism, piston drive mechanism is configured to the contact piston assembly, thereby the piston rod of piston component is moved in most advanced and sophisticated retainer, regulate the amount of liquid in the most advanced and sophisticated retainer whereby, the amount of liquid that volume required representative will be regulated; Utilize volume characterization calculation correction volume, wherein volume characterization is poor as the amount of liquid of regulating in most advanced and sophisticated retainer of volume required function, and volume characterization utilizes calibration process to determine; And, regulate the required liquid volume in the most advanced and sophisticated retainer whereby to the correction volume that the user shows pipette.
Another example embodiment of the present invention relates to a kind of current physical state by the correction pipette and comes the device of the required liquid volume in the actuated pipette of regulator solution.This device includes, but is not limited to: main body, most advanced and sophisticated retainer, piston component, piston drive mechanism, volume selector, display and processor.Most advanced and sophisticated retainer is installed on the main body.Piston component is installed on the most advanced and sophisticated retainer, and includes, but is not limited to be assemblied in the piston rod in the most advanced and sophisticated retainer.Piston drive mechanism includes, but is not limited to have the control lever on the surface of contact piston assembly.Piston drive mechanism is configured to the piston rod of mobile piston assembly in most advanced and sophisticated retainer, regulates the liquid in the most advanced and sophisticated retainer whereby.The volume selector is installed on the main body, and is configured to allow the user to select required volume.The amount of liquid that volume required representative will be regulated.Display is installed on the main body.Processor is coupled on display and the volume selector, and is configured to utilize volume characterization calculation correction volume.Volume characterization is poor as the amount of liquid of regulating in most advanced and sophisticated retainer of volume required function.Volume characterization utilizes calibration process to determine.Display is regulated the required liquid volume in the most advanced and sophisticated retainer whereby to the correction volume that the user shows pipette.
After accompanying drawing, detailed description and the appending claims below reading, other principal character of the present invention and advantage will become apparent for a person skilled in the art.
The accompanying drawing summary
Describe preferred embodiment subsequently with reference to the accompanying drawings, wherein identical Reference numeral is represented identical parts.
Fig. 1 is the sectional view according to the electronic pipette of an example embodiment of the present invention.
Fig. 2 is the sectional view of piston drive mechanism, piston component, most advanced and sophisticated retainer and the exterior tip pushing tow top-pushing mechanism of the electronic pipette of Fig. 1.
Fig. 3 is the sectional view according to the on-electric pipette of an example embodiment of the present invention.
Fig. 4 is the sectional view that is connected to the on-electric pipette of the Fig. 3 on the prover according to the present invention's first example embodiment.
Fig. 5 is the flow chart of exemplary calibration operations of the pipette of Fig. 4.
Fig. 6 be expression in the pipette actual volume and the chart of the regulating error between the correction volume.
Fig. 7 is the first demonstration table of expression according to the volume characterization of an example embodiment of the present invention.
Fig. 8 is the second demonstration table of expression according to the volume characterization of an example embodiment of the present invention.
Fig. 9 is the chart of regulating error between actual volume in the pipette that is illustrated under the different operating condition and the correction volume.
Figure 10 is the flow chart according to the demonstration of the pipette of an example embodiment of the present invention.
Figure 11 is the sectional view according to the piston drive mechanism of the on-electric pipette of Fig. 3 of second example embodiment.
Figure 12 is the sectional view according to the piston drive mechanism of the on-electric pipette of Fig. 3 of the 3rd example embodiment.
Figure 13 is according to the most advanced and sophisticated retainer of the on-electric pipette of Fig. 3 of the 4th example embodiment and the sectional view of housing on every side.
Figure 14 is the sectional view according to the sensor of the 4th example embodiment of Figure 13.
Figure 15 is according to the piston drive mechanism of the on-electric pipette of Fig. 3 of the 5th example embodiment and the sectional view of piston component.
Figure 16 is the side view according to the piston component of the on-electric pipette of Fig. 5 of the 5th example embodiment.
Detailed description of preferred embodiment
As used in this open text, term " installation " comprises joint, associating, connection, combination, inserts, hangs, keeps, attaches, adheres to, fastening, bundle clothes, paste, fixing, screw, screw, rivet, welding, weld seam and other similar terms.Term " adjusting " comprises the absorption and/or the distribution of the liquid in the pipette.With reference to the example embodiment of Fig. 1 and 2, electronic pipette 30 can be accepted instruction and automatically a series of liquid volumes be drawn and are assigned in one or more most advanced and sophisticated retainers 36.Electronic pipette 30 comprises many elements and subsystem, and these elements and subsystem provide the multiple mode of operation of drawing and distributing the liquid of precise volumes together.The element of electronic pipette 30 and subsystem include, but is not limited to housing 32, piston drive mechanism 34, piston component 35, most advanced and sophisticated retainer 36, internal power subsystem 38, external tip ejection mechanism 40, internal tip ejection mechanism 42, control electronic cards 44, display 170 and volume selector 172.Some of them element and subsystem are well known to a person skilled in the art, therefore need not herein to describe in detail.Housing 32 is hollow normally, and as the location reference of other element of pipette 30.Most of pipette components directly or indirectly is installed on the housing 32.Housing 32 so that control pipette 30, and becomes one of pipette part of direct contact user hand when the operation pipette for the user provides handle thus.
Motor 70 is mobile control lever 72 under the control that is installed to the processor on the control electronic cards 44.Motor 70 can utilize and well known to a person skilled in the art that various electromechanical assemblies carry out.Motor 70 makes control lever 72 accurately the moving up and down of longitudinal axis A-A, thereby liquid is sucked or be assigned in the most advanced and sophisticated retainer 36 or draw from most advanced and sophisticated retainer 36 or distribute.Motor 70 and the processor interface of controlling electronic cards 44, motor 70 receives the signal of telecommunication that is used to control control lever 72 displacements from control electronic cards 44.Control electronic cards 44 can comprise one or more connectors or the interface with motor 70 communications.Control lever tip 74 is installed to the end with motor 70 opposed control levers 72.For example, control lever tip 74 can be screwed on the control lever 72 or within.Control lever support 76 retentive control bars 72 are along the displacement of longitudinal axis A-A.Shell 78 is installed on the control lever support 76, and surrounds a part of control lever 72 and the control lever tip 74 that extends to outside the control lever support 76 and form socket.
With reference to the example embodiment of Fig. 2, piston component 35 includes, but is not limited to piston head 92, piston rod 94, piston shell 96, piston reset spring 98 and spring guide 100.Piston head 92 can be the disk that forms with metal or plastic material.Piston head 92 has first 91.Piston rod 94 is installed on the piston head 92, and with the direction of first 91 opposed approximate vertical of piston head 92 on extend.Piston rod 94 has and is generally columniform shape.
Piston shell 96 is installed on the piston head 92, with the direction of first 91 opposed approximate vertical of piston head 92 on extend, and surround piston rod 94.Piston shell 96 has and is generally columniform shape, and can comprise one or more tapered cross-sections.Piston reset spring 98 is installed on the piston shell 96, and along longitudinal axis A-A with the direction of first 91 opposed approximate vertical of piston head 92 on extend.In an example embodiment, piston reset spring 98 slides above piston shell 96, and is held in place by means of the piston reset spring 98 and the frictional force of adjoining between piston shell 96 cross sections of piston head 92.Piston component 35 slips into the shell 78 interior (as shown in Figure 2) of piston drive mechanism.
Shown in the example embodiment of Fig. 2, most advanced and sophisticated retainer 36 includes, but is not limited to pipe 110, manages 112 and O shape ring 114 down.Under manage 112 be installed to the pipe 110 on.For example, following pipe 112 can comprise the end of thread in the complementary threads surface that is screwed to pipe 110.Last pipe 110 and following pipe 112 can comprise one or more tapered cross-sections.In the lower cut of O shape ring 114 between last pipe 110 and following pipe 112.O shape ring 114 piston rod 94 with manage 112 down between provide watertight to be connected.Pipe hold-down nut 84 slides above most advanced and sophisticated retainer 36, and most advanced and sophisticated retainer 36 is pressed on the piston component 35, fixes most advanced and sophisticated retainer 36 with respect to housing 32 and piston drive mechanism 34 whereby.
First 91 of piston component 35 in the shell 78 of the most advanced and sophisticated 74 contact piston driving mechanisms 34 of control lever.When dispense liquid, piston drive mechanism 34 is by the displacement of control lever tip 74 along longitudinal axis A-A, and piston component 35 that first 91 of most advanced and sophisticated 74 contact of control lever pushed open from piston drive mechanism 34.Piston reset spring 98 is pressed against on the spring guide 100 that is held in place by pipe hold-down nut 84.When imbitition, piston drive mechanism 34 is shifted to piston drive mechanism 34 with control lever tip 74.Because the compression force of piston reset spring 98 causes first 91 no matter this displacement still contacts with control lever most advanced and sophisticated 74.
External tip ejection mechanism 40 and internal tip ejection mechanism 42 be most advanced and sophisticated 130 absorption and distribution end pushing tows from pipette 30, thereby avoid the contingent pollution of sample.Internal tip ejection mechanism 42 includes, but is not limited to ejection knob 140, stationary cylinder 142, button cylinder 144, cylinder body 146, bar 148, goring push spring 150 and mounting column 152.Stationary cylinder 142 is installed on the housing 32.Mounting column 152 is installed on housing 32 and/or the stationary cylinder 142.Stationary cylinder 142 and mounting column 152 still are fixed on the housing 32.Ejection knob 140 is installed on the button cylinder 144.Ejection knob 140 can utilize user's the left hand or the right hand to carry out comfortable operation around longitudinal axis A-A rotation whereby.Button cylinder 144 is installed on the stationary cylinder 142 slidably, moves to allow button cylinder 144, and ejection knob 140 is depressed simultaneously, with pushing tow tip 130.Cylinder body 146 is installed on the button cylinder 144.Bar 148 is installed on the end with button cylinder 144 opposed cylinder bodies 146.Goring push spring 150 is installed on the cylinder body 146 at first end 156, is installed on the mounting column 152 at second end 158.Depressing of ejection knob 140 drives bar 148 to most advanced and sophisticated 130.Goring push spring 150 causes bar 148 to return in the opposite direction, when ejection knob 140 discharges ejection knob 140 is moved back into the home position whereby.
With reference to Fig. 2, external tip ejection mechanism 40 includes, but is not limited to ejection blade 156 and ejection blade adjusting knob 158.Ejection blade 156 has the curved shape of the outer shape of following most advanced and sophisticated retainer 36.Ejection blade 156 has first end 160 and second end 162.Second end 162 is included in the enclosed cylinder of sliding in most advanced and sophisticated retainer 36 tops.As a result, depressing of ejection knob 140 causes ejection blade 156 to move along most advanced and sophisticated retainer 36, therefore utilizes second end 162 from most advanced and sophisticated retainer 36 pushing tow tips 130.Be installed to the rotation of the pushing tow adjusting knob 158 near the ejection blade 156 first end 160, cause second end 162 of ejection blade 156 to move or to move down on the most advanced and sophisticated retainer 36.Regulate the position of ejection blade 156 along most advanced and sophisticated retainer 36, make the various types of tips of external tip ejection mechanism 40 pushing tows.
Pipette 30 can comprise the communication interface with the computing equipment communication.Computing equipment can be any type of computer, and these forms comprise desktop type, on knee, personal digital assistant type etc.Computing equipment is physically away from pipette 30.Communication interface can be positioned at the top with most advanced and sophisticated 130 opposed housings 32, so that the user touches easily, and need not to interrupt the operation of pipette 30.Communication between pipette 30 and the computing equipment can utilize multiple transmission technology, and global system (GSM), universal mobile telecommunications system (UMTS), time division multiple acess that these technology include, but is not limited to CDMA connection (CDMA), mobile communication connect (TDMA), transmission control protocol/Internet protocol (TCP/IP), short message service (SMS), many media communications service (MMS), Email, instantaneous communication service (IMS), bluetooth, IEEE 802.11 etc.Pipette 30 can utilize multiple media to come communication with computing equipment, and these media include, but is not limited to radio, infrared, laser, cable connection etc.Thus, communication interface can utilize wired connection and/or wireless connections.
Wired connection can comprise first end that links to each other with the communication interface of pipette 30 and second end that links to each other with the communication interface of computing equipment.In an example embodiment, the communication interface of pipette 30 satisfies 1394 small-sized standards of Institute of Electrical and Electronics Engineers (IEEE).In an example embodiment, the communication interface of computing equipment can be for receiving RS 232 types that the universal serial bus connector designs.In yet another embodiment, the communication interface of the communication interface of pipette 30 and/or computing equipment can be an Ethernet interface.
Wireless communication interface can connect from being short to the equipment of long a plurality of distances.Pipette 30 and computing equipment can support to broadcast and receive the processing of wireless signal.Wireless signal can be such as adopting IEEE 802.11
TMStandard is utilized 802.11a, 802.11b, 802.11f or 802.11g version.In addition, wireless signal can be such as adopting bluetooth standard, and wherein IEEE 802.15.1 is a latest edition.IEEE 802.11
TMThe wireless standard of specification regulation WLAN (WLANs), this network provide " overhead (over-the-air) " interface between wireless client and base station or access point and between other wireless client.IEEE 802.15 working groups provide those that low-complexity and low power consumption wireless personal-area network (PANs) for example support by bluetooth specification.
With reference to Fig. 3, in an example embodiment, express the sectional view of on-electric pipette 200.The element of on-electric pipette 200 and subsystem include, but is not limited to the indicator 256 at volume selector 202, housing 204, piston drive mechanism 206, piston component 208, most advanced and sophisticated retainer 210, battery 212, tip ejection mechanism 214, display 216, control electronic cards 218 and a kind of tip.Some of them element and subsystem are well known to a person skilled in the art, therefore need not herein to describe in detail very much.Volume selector 202 comprises knob 220 and regulates bolt 222.The rotation knob 220 cause regulating bolt 222 longitudinally A-A move up and down, change required absorption or dispensed volume whereby.
The rotation of knob 220 causes the translation of control lever 226.Control lever tip 228 is installed on the end with knob 220 opposed control levers 226.For example, control lever tip 228 can be screwed on the control lever 226 or within.Control lever support 230 is kept the displacement of control lever 226 along longitudinal axis A-A.Shell 232 is installed on the control lever support 230, thereby and is enclosed in outside the control lever support 230 and extends part control lever 226 and the control lever tip 228 that forms socket.
Piston component 208 includes, but is not limited to piston head 236, piston rod 224, piston shell 240, piston reset spring 242 and spring guide 244.Piston head 236 can be the disk made from metal or plastic material.Piston head 236 has first 246.Piston rod 224 is installed on the piston head 236, and with the direction of first 246 of piston head 236 opposite approximate vertical on extend.Piston rod 224 has and is generally columniform shape.
Piston shell 240 is installed on the piston head 236, with the direction of first 246 of piston head 236 opposite approximate vertical on extend, and surround piston rod 224.Piston shell 240 has and is generally columniform shape, can comprise one or more tapered cross-sections.Piston reset spring 242 is installed on the piston shell 240, and along longitudinal axis A-A with the direction of first 246 of piston head 236 opposite approximate vertical on extend.In an example embodiment, piston reset spring 242 slides above piston shell 240, and is held in place by means of the piston reset spring 242 and the frictional force of adjoining between the cross section of piston shell 240 of piston head 236.When assembling, piston component 208 slips into the shell 232 interior (as shown in Figure 3) of piston drive mechanism 206.
Shown in the example embodiment of Fig. 2, most advanced and sophisticated retainer 210 includes, but is not limited to pipe 248, manages 250 and O shape ring 252 down.Under manage 250 be installed to the pipe 248 on.For example, following pipe 250 can comprise the end of thread in the complementary threads surface that is screwed to pipe 248.Last pipe 248 and following pipe 250 can comprise one or more tapered cross-sections.In the lower cut of O shape ring 252 between last pipe 248 and following pipe 250.O shape ring 252 piston rod 224 with manage 250 down between provide watertight to be connected.Pipe hold-down nut 254 press against most advanced and sophisticated retainer 210 on the piston component 208 above slide, whereby with respect to housing 204 and piston drive mechanism 206 fixing most advanced and sophisticated retainers 210.
First 246 of piston component 208 in the shell 232 of the most advanced and sophisticated 228 contact piston driving mechanisms 206 of control lever.When dispense liquid, piston drive mechanism 206 is by the displacement of control lever tip 228 along longitudinal axis A-A, and in that of first 246 of most advanced and sophisticated 228 contact of control lever piston component 208 pushed open from piston drive mechanism 206.Piston reset spring 242 is pressed against on the spring guide 244 that is held in place by pipe hold-down nut 254.When imbitition, piston drive mechanism 206 moves control lever tip 228 to piston drive mechanism 206.No matter this displacement, first 246 compressive force owing to piston reset spring 242 still keeps in touch with control lever tip 228.
With reference to Fig. 4, pipette 200 is connected on the balance 260 by line 262 in calibration process.Line 262 is connected on the pipette 200 by terminal 264, directly carries out data communication between balance 260 and the pipette memory thereby make.For example, terminal 264 can be a RS232 type connector.In yet another embodiment, pipette 30 can use in a similar fashion.
With reference to Fig. 5, this figure is the demonstration flow chart of calibration process that utilizes the pipette 200 of Fig. 4.In operation 278, utilize balance 260 to determine the empty-weight of pipette 200.In operation 280, the user selects correction volume, so that regulate on pipette 200.In operation 282, the user utilizes pipette 200 to draw required volume.In operation 284, utilize balance 260 to measure the sucking weight of the pipette 200 that comprises institute's imbibition body.In operation 286, based on the sucking weight of pipette 200 and the difference of the empty-weight of pipette 200 and the physical characteristic that well known to a person skilled in the art sucked liquid, the calculating actual volume aspirated.In operation 288, send the actual volume that is calculated to pipette 200, it is stored in the memory that contains correction volume herein.For example, correction volume can be stored in well known to a person skilled in the art database or form with the actual volume aspirated of being measured.In operation 290, test definitely, whether should draw extra correction volume.If determine it is sure, repetitive operation 280-288 then is so that draw extra correction volume.In yet another embodiment, calculate empty-weight at each extra correction volume.In operation 292,, then determine with the amount of liquid difference that is conditioned volume characterization as the correction volume function if use extra correction volume.
With reference to Fig. 6, the figure shows out desirable response curve 294 and measure response curve 296.The ideal pipette of correction volume is accurately drawn in desirable response curve 294 expressions.Measure the real response of response curve 296 expression pipettes.Curve 294 is represented regulating error with the difference of curve 296.For example, in the calibration process that reference Fig. 5 describes, select three correction volume A, B and C.Based on correction volume A, in calibration process, measure actual volume A
aRegulating error 298 is correction volume A and actual volume A
aDifference or A
a-A.Equally, regulating error 300 is correction volume B and actual volume B
aDifference or B
a-B.Moreover regulating error 302 is correction volume C and actual volume C
aDifference or C
a-C.
Correction volume is A and corresponding actual volume aspirated A for example
aLimit calibration data point.The calibration data point of using in calibration process is many more, and the approximation of the measurement response curve 296 that calculates is just accurate more.Technical staff as simulation field is known, can adopt several different methods, utilizes the correction volume data that comprise correction volume and regulating error or actual volume aspirated, comes approximate measure response curve 296.Can utilize in these methods any to determine volume characterization, so that determine to measure the volume rather than the correction volume of response curve 296.
For example, utilize multiple curve match formula that best fit with one group of data point is provided.The output of curve match formula is an equation.For example, can utilize n
ThThe level multinomial comes approximate measure response curve 296 with the calibration data point at A, B and C place.Thus, utilize one or more data points, determine volume characterization.For example, volume characterization can be the equation with the definition of curve match formula.If an equation is not suitable for analogue measurement response curve 296, just define the response that extra equation limits two required volume aspirated between the correction volume.For example, based on measuring response curve 296, linear equation is fit to the volume greater than B.Yet for the volume less than B, multinomial is approximate measure response curve 296 better.In this case, volume characterization comprises two equations.
Perhaps, volume characterization can be the form that comprises a plurality of calibration data point.Determining of actual volume aspirated is by utilizing predetermined party formula interpolation or extrapolate from calibration data point and to realize between calibration data point.Perhaps, be used for interpolation and/or can be included in form from the equation of calibration data point extrapolation.As well known to a person skilled in the art, data can be captured in the form.Form can be any type of, includes, but is not limited to the form with file or database formal definition.With reference to Fig. 7, explanation the figure shows form 304 for example, and this form is expressed the regulating error as the function of correction volume A, B and C.In actual volume aspirated and the regulating error any or the two can both be stored in the form 295.
Or, in form shown in Figure 8 306, comprise equation index (indicator) and the related content used with equation.For example, the equation index is to represent in 1 o'clock, only uses " constant 1 " to describe the linear equation of the measurement response curve 296 between each correction volume.The equation index is to represent in 3 o'clock, describes the multinomial equation of secondary of the measurement response curve 296 between each correction volume with " constant 1 ", " constant 2 " and " constant 3 ".Thus, equation 2.7+0.5A+0.01A
2Define volume required correction volume less than A.Equation 4.6-1.6A defines greater than A and less than the volume required correction volume of B.Equation 2.9+8.9A defines the volume required correction volume greater than B.
In an example embodiment, define volume characterization with two correction volume at least.Preferably, one of two correction volume are the minimum operation volumes of pipette, and another is the maximum operational volume of pipette.Liquid weight in mechanical faults (especially bolt displacement path and piston diameter) and the most advanced and sophisticated retainer is considered in the correction that utilizes the maximum volume of pipette to carry out to greatest extent.Mechanical faults and capillarity are considered in the correction that utilizes the minimum volume of pipette to carry out to greatest extent.By utilizing extra correction volume can improve precision.Exactly relevant is that multiple interpolating method as known in those skilled in the art, can be used to determine and the unequal volume required correction volume of one or more correction volume in the pipette operating process.
One or more equations and/or form can be used to define volume characterization based on the additional physical state of pipette.For example, based on the type definable first party formula and/or the form of pipette.At specific pipette definable second party formula and/or form, because the practical measurement volume can be different according to manufacturing tolerance, this tolerance allows the element between pipette and the pipette to change in manufacture process.
As additional example, when operating in the environment of pipette in different atmospheric temperatures, measuring response curve 296 can change.With reference to Fig. 9, the figure shows out three and measure the response curve example.For example, measuring response curve 308 is to limit under 10 ℃ atmospheric temperature.Measuring response curve 310 is to limit under 20 ℃ atmospheric temperature.Measuring response curve 312 is to limit under 25 ℃ atmospheric temperature.As a result, different equation or set of equations or form can be used to define each and measure response curve 308,310 and 312.Thus, volume characterization is utilized parameter and is volume requiredly determined correction volume.As known in those skilled in the art, can utilize several different methods between a plurality of curves, to carry out interpolation.Thus, represent the parameter of the current physical state of pipette to can be used to further define the volume characterization of pipette, other deviation source of proofreading and correct the volume of being drawn whereby.
Parameter includes, but is not limited to be used for the most advanced and sophisticated kind of pipette, the atmospheric temperature of pipette, the temperature of part pipette, atmospheric pressure, the pressure in the pipette chamber, the atmospheric humidity of pipette and the liquid viscosity that will regulate of pipette.The pipette user can utilize the indicator 256 of most advanced and sophisticated kind to select to be positioned at most advanced and sophisticated kind on the most advanced and sophisticated retainer 210.Tip with different size and shape can produce different measurement response curves.Thus, the most advanced and sophisticated kind that is installed on the most advanced and sophisticated retainer can change volume characterization.In addition, the one or more sensors (shown in Figure 11-16) that are installed on the pipette can be used to provide the parameter of using with volume characterization, so that based on the atmospheric pressure of the temperature of the atmospheric temperature of pipette, part pipette, pipette, pressure in the pipette chamber and the atmospheric humidity calculation correction volume of pipette.Sensor or indicator also can be indicated kind of liquid to be regulated.In this case, volume characterization comprises mainly based on the correction of liquid viscosity to selected liquid.
Usually, the volume characterization that the parameter of utilizing sensor to measure is determined is regulating error " C ", and this error is to be carried out and accepted the predetermined mathematical equation of atmospheric pressure, atmospheric temperature and these location parameters of atmospheric humidity by processor.In an example embodiment, " C " is calculated as: C=a*B+m, wherein " B " is volume required, " a " and " m " is predetermined correction value.The value of " m " can be zero.Parameter " a " can be defined by following formula:
a＝(1-D
atm/e)/(D
i-D
atm)
D
iAnd D
AtmBe respectively the density value that is conditioned liquid and air, " e " is constant.
Density D
iCalculate with predetermined mathematical equation with temperature of measuring by atmosphere temperature transducer.In this example, D
i=g/f (T
i), wherein " g " is constant, " T
i" be the temperature of being measured, " f (T
i) " be predetermined polynomial function.For example
D
i＝1000/(999.87-0.06426T
i+0.0085045T
i 2-0.0000679T
i 3)
In this formula, T
iBe degree centigrade D
iBe kilogram/rice
3
In a similar fashion, D
AtmCalculate with predetermined mathematical formula with variable atmospheric pressure, atmospheric temperature and atmospheric humidity of measuring by sensor.In this example,
D
atm＝45P
atm/(12908(T
i+273.15))+(T
i-0.02H)1000
P
AtmBe pressure (Pascal), H is a moisture percentage.For example, H is 40% humidity (0.4).
With reference to Figure 10, the figure shows based on the volume characterization of in calibration process, determining and proofread and correct the demonstration that pipette liquid is in use regulated.In operation 320, the user utilizes volume required that pipette selects to regulate.In operation 322, definite parameter of representing the current physical state of pipette.Utilization is stored in the volume characterization in the pipette memory, at operation 324 usefulness processor calculation correction volumes.Volume characterization determine as the function of volume required and/or parameter to be conditioned amount of liquid poor, thus correction volume represent as the function of volume required and/or parameter to be conditioned amount of liquid poor.In operation 326, volume requiredly be shown to the user.
In operation 328, correction volume is shown to the user.According to such as formula in the interpolation that defines between two volume required more accurate data points, correction volume can be actual volume aspirated or volume required regulating error.In operation 330, the user can select new volume required based on shown correction volume.For example, if pipette is not electronic, then display can show correction volume and volume required.The user correspondingly selects new volume required, up to correction volume within user's permissible accuracy with volume required coupling till.
In yet another embodiment, correction volume is shown as volume required, thereby is transparent based on the volume required and process parameter correction pipette to the user.Thus, for example, utilize electric pipette 20, the position of processor automatically calibrating control lever is to comprise regulating error.Display shows it also is the volume required of correction volume because processor is adjusted the position of control lever, thereby regulate volume required, although it comprises the physical state of pipette 20 and volume required effect.Thus, with display be in the mode of operation of feature from dynamic(al) correction, volume required demonstration merges correction automatically.Display is regulated with the physics of pipette and is changed, and the user need not to carry out any adjustment.
High/low index is shown to the user of pipette in operation 332.High/low index shows, regulating error is positive or negative.High/low index can be a minus sign, if the danger of useful quantity not sufficient when actual volume is higher than more accurate volume, high/low index can be a plus sige also, if excessive danger is arranged when actual volume is lower than more accurate volume.In a word, on the display of pipette, can provide three information: volume required, correction volume and high/low index to the user.Correction volume can be regulating error or actual volume.Utilize this information, the user can adjust volume required, demonstrates till the positive sign or negative sign that is equating within the trueness error between expression volume required and the correction volume up to high/low index.In yet another embodiment, high/low index is not presented on the pipette.
Whether in another embodiment, adopt and proofread and correct the process that is conditioned volume, be by the selectable optional project of user.Thus, the user can select command processor not comprise any corrected value, because the user utilizes pipette to regulate liquid.In yet another embodiment, the processor of pipette can be programmed, so that only proofread and correct greater than such as accuracy value the time at corrected value.Select the predetermined precision value, so that distinguish the situation that comprises the situation of remarkable correction and do not comprise remarkable correction.Such embodiment makes the user carry out maximum control to the operation of pipette.
Calibration process shown in Figure 5 and use shown in Figure 10, proofread and correct the amount of liquid that is conditioned of pipette owing to following phenomenon: the bad manufacturing of the volume required lower capillarity that causes, the volume required fluid column weight that greatly causes, pipette components, most advanced and sophisticated kind and the physical condition of pipette, comprise temperature, the pipette of part pipette atmospheric pressure, the pressure in the pipette chamber, pipette atmospheric humidity and be conditioned the viscosity of liquid.For example, when the user handles time of pipette when longer, pipette is heated owing to contacting with user's hand, thereby the operation of pipette changes owing to the thermal expansion of some elements.According to the process of being summarized, the precision of pipette can be maintained, because pipette heating, and in fact all keeps the precision of pipette under a lot of pipette operating conditions.Pipette can be adjusted automatically.Or shown correction volume can indicate variation to the user, and the user can manually adjust pipette.Because pipette is proofreaied and correct after making, the required required precision in therefore pipette being made is not high.As a result, pipette can more easily create with lower cost.Also reduced the multiple expansion of mechanical system, these expand and often cause directly influencing the sampling displacement volume drift of the amount of being got.Consider a plurality of physical conditions, can comprise continuous correction.
With reference to Figure 11-16, shown exemplary sensor configurations provides the parameter as the input value of volume characterization.With reference to Fig. 8, the figure shows second example embodiment of pipette 200.Pipette also comprises the temperature sensor 340 of the installation of the shell 232 that adjoins piston drive mechanism 206.The location of temperature sensor 340 is in order to measure the temperature of part pipette 200.In this example embodiment, temperature sensor 340 is near control lever 226, control lever support 230 and control lever electronic cards 218.Control electronic cards 218 may produce the heat of some elements expansions that cause piston drive mechanism 206.Temperature sensor 340 is installed near the thermal expansion the best part, thus, the temperature of mechanical organ is included in treats in the known sampling sequence.Temperature sensor 340 links to each other with control electronic cards 218 via electric connection line 342, so that processor is adjusted the temperature that sensor is measured.
Adjustment is to utilize the temperature of being measured by sensor to carry out according to process shown in Figure 11 as parameter.In calibration process, pipette is generally operated at about 20 ℃ absorption/dispense liquid.If the user regulates not at 20 ℃ liquid, the volume that then is conditioned does not just correspond to the show value on the pipette display.The volume of removing is owing to multiple reason is different from required volume.Cause the main cause of this error to be, " extremely " volume of pipette inside warm, pipette causes the user to regulate/adjust to such an extent that lack the adjusting of liquid than expecting owing to expanding.Known, provided pipette calibration explanation for 20 ℃ pipettes, particularly set those explanations of specified sample volume, processor determines, the temperature that the correction of this rated value is measured according to temperature sensor 340 and whether be necessary based on the volume characterization of this temperature.
With reference to Figure 12, the figure shows out the 3rd example embodiment of pipette 200.Pipette also comprises the atmosphere pressure sensor 350 that adjoins piston drive mechanism 206 installations.In this example, sensor is installed in battery 212 tops.Atmosphere pressure sensor 350 is measured atmospheric pressure, and sends this information to processor, so that such volume that is conditioned of proofreading and correct as previously involved.Pipette also can comprise near the most advanced and sophisticated retainer atmosphere outside temperature sensor 352 that is installed in most advanced and sophisticated.At this position, atmosphere temperature transducer 352 is measured near the temperature that is conditioned the liquid, even the sensor air of contact liq top only, this temperature also is similar to the temperature of liquid very much.The surface of atmosphere temperature transducer 352 puncture tip retainers, pipe 356 in entering is to measure near the atmospheric temperature that is conditioned liquid.Atmosphere temperature transducer 352 links to each other with control electronic cards 218 via electric connection line 354, so that the temperature that processor is measured at sensor is adjusted.In another embodiment, humidity sensor is installed on the pipette and atmosphere pressure sensor 350 similar positions.
With reference to Figure 13, the figure shows out the 4th example embodiment of pipette.Pipette also comprises the atmosphere temperature transducer 360 on the following pipe that is installed in most advanced and sophisticated retainer 210.Atmosphere temperature transducer 360 forms at the following pipe of most advanced and sophisticated retainer and allows it to be received in a ring in the cylindrical shell that is arranged on most advanced and sophisticated retainer lower end.Atmosphere temperature transducer 360 has the same inwall of annulus, forms the curve be arranged in the circle thus, this circle be centered close to the opposed wall of pipette longitudinal axis A-A on (as shown in figure 14).Formed narrow air duct increases the air velocity of traversing atmosphere temperature transducer 360, and allows to remove liquid by pushing tow.Atmosphere temperature transducer 360 links to each other with control electronic cards 218 via electric connection line 362, so that the temperature that processor is measured at sensor is adjusted.
With reference to Figure 15, the figure shows out the 5th example embodiment of pipette.Pipette also comprises the atmosphere temperature transducer 370 on the movable part that is installed within the pipette.Atmosphere temperature transducer 370 directly is fixed on an end of piston rod 224.At this position, atmosphere temperature transducer 370 does not contact the liquid that is conditioned.Atmosphere temperature transducer 370 links to each other with control electronic cards 218 via electric connection line 372, so that the temperature that processor is measured at sensor is adjusted.With reference to Figure 16, electric connection line 372 with link to each other perpendicular to pipette longitudinal axis A-A, two metal tapes 374,376 that are laminated on another, and at piston crown.Metal tape 374,376 contacts with two blades 378,380 that are installed in pipette body case respectively.This set makes between processor and the atmosphere temperature transducer 370 to be realized nonvolatilly electrically contacting, and no matter whether piston rod rotates.
Example embodiment of the present invention instructs pipette to utilize various viscous liquids and tip effectively, under various environmental operations conditions, accurately draws or the dispense liquid volume, and no matter the mechanical faults in the pipette manufacturing.Demonstration can utilize on-electric or electric pipette to implement.Should be appreciated that the present invention is not limited to this paper as the specific embodiment that illustrates, and covers all such modifications, combination and exchange but contain, in the scope of these appending claims that all lag behind.Described function can be distributed in the different parts of number, and function as herein described distribution does not break away from marrow of the present invention.In addition, under the prerequisite that does not break away from marrow of the present invention, the execution sequence of module can change.Thus, the description of preferred embodiment is for the purpose that illustrates, and does not have the qualification effect.
Claims (26)
1, a kind of current physical state by the correction pipette comes the method for the required liquid volume in the actuated pipette of regulator solution, and described method comprises:
On pipette, select required volume, described pipette comprises piston drive mechanism, described piston drive mechanism is configured to the contact piston assembly, thereby the piston rod of described piston component is moved in most advanced and sophisticated retainer, regulate the amount of liquid in the described most advanced and sophisticated retainer whereby, the amount of liquid that volume required representative will be regulated;
Utilize volume characterization calculation correction volume, wherein said volume characterization is poor as the amount of liquid of regulating in described most advanced and sophisticated retainer of volume required function, and described volume characterization utilizes calibration process to determine; And
User to pipette shows described correction volume, regulates the required liquid volume in the described most advanced and sophisticated retainer whereby.
2, the method for claim 1 is characterized in that, also comprises:
Determine parameter, described parameter is represented the current physical state of pipette;
Wherein said volume characterization is still poor as the amount of liquid of regulating in described most advanced and sophisticated retainer of described parametric function.
3, method as claimed in claim 2 is characterized in that, described parameter is most advanced and sophisticated kind.
4, method as claimed in claim 2 is characterized in that, described parameter is measured with the sensor that is installed on the pipette.
5, method as claimed in claim 4, it is characterized in that described parameter is selected from the group that is made of following parameter: the pressure in the temperature of the atmospheric temperature of pipette, part pipette, the atmospheric pressure of pipette, the pipette chamber, the atmospheric humidity and the liquid viscosity of pipette.
6, method as claimed in claim 2 is characterized in that, also comprises the user who is shown to pipette with volume required.
7, the method for claim 1 is characterized in that, described correction volume is an actual volume, and described actual volume is represented based on the volume required amount of liquid of regulating in described most advanced and sophisticated retainer.
8, the method for claim 1, it is characterized in that, described correction volume is a regulating error, and described regulating error is represented the poor of volume required and actual volume, and described actual volume representative is based on the volume required amount of liquid of regulating in described most advanced and sophisticated retainer.
9, method as claimed in claim 8 is characterized in that, also comprises: the user selects new volume on pipette, and wherein said new volume comprises regulating error.
10, method as claimed in claim 8 is characterized in that, also comprises: high/low index is shown to the user, and described high/low index shows that regulating error is positive or negative.
11, the method for claim 1 is characterized in that, described volume characterization is a form, and described form comprises:
A plurality of data points, wherein each data point comprises:
Calibrated volumes data point, wherein said calibrated volumes data point are represented the liquid volume that will regulate, and described calibrated volumes data point is selected as the part calibration process of pipette; And
Correction volume, wherein said correction volume are illustrated in the amount of liquid of regulating in the described most advanced and sophisticated retainer at calibrated volumes data point place.
12, the method for claim 1 is characterized in that, described volume characterization is a form, and described form comprises:
A plurality of data points, wherein each data point comprises:
Calibrated volumes data point, wherein said calibrated volumes data point are represented the liquid volume that will regulate, and described calibrated volumes data point is selected as the part calibration process of pipette; And
Correction volume, wherein said correction volume are represented the poor of described calibrated volumes data point and actual volume, and wherein said actual volume is illustrated in the amount of liquid of regulating in the described most advanced and sophisticated retainer at described more accurate volume data point place.
13, the method for claim 1 is characterized in that, described volume characterization is an equation.
14, a kind of current physical state by the correction pipette comes the device of the required liquid volume in the actuated pipette of regulator solution, and described device comprises:
Housing;
Most advanced and sophisticated retainer, described most advanced and sophisticated retainer is installed on the described housing;
Piston component, described piston component are installed on the described most advanced and sophisticated retainer, and comprise the piston rod that is assemblied in the described most advanced and sophisticated retainer;
Piston drive mechanism, described piston drive mechanism comprises the control lever on the surface with the described piston component of contact, described piston drive mechanism is configured to move the described piston rod of described piston component in described most advanced and sophisticated retainer, regulate the liquid in the described most advanced and sophisticated retainer whereby;
The volume selector, described volume selector is installed on the described housing, and is configured to allow the user to select required volume, the amount of liquid that volume required representative will be regulated;
Display, described display are installed on the described housing;
Processor, described processor is coupled on described display and the described volume selector, and be configured to utilize volume characterization calculation correction volume, wherein said volume characterization is poor as the amount of liquid of regulating in described most advanced and sophisticated retainer of volume required function, and described volume characterization utilizes calibration process to determine;
Wherein said display shows described correction volume to the user of pipette, regulates the required liquid volume in the described most advanced and sophisticated retainer whereby.
15, device as claimed in claim 14 is characterized in that, also comprises:
The physical state indicator, described physical state indicator is installed on a part of device, and is configured to the current physical state of display unit;
The coupling of wherein said processor and described physical state indicator, and described volume characterization is still poor as the amount of liquid of regulating in described most advanced and sophisticated retainer of the function of shown current physical state.
16, device as claimed in claim 15 is characterized in that, also comprises:
Tip, described tip are installed on the described housing;
Wherein said physical state indicator is most advanced and sophisticated kind indicator.
17, device as claimed in claim 15 is characterized in that, described physical state indicator is a sensor.
18, device as claimed in claim 17, it is characterized in that described current physical state is selected from the group that is made of following parameter: the pressure in the temperature of the atmospheric temperature of pipette, part pipette, the atmospheric pressure of pipette, the pipette chamber, the atmospheric humidity and the liquid viscosity of pipette.
19, device as claimed in claim 14 is characterized in that, described display also is configured to the user that is shown to pipette with volume required.
20, device as claimed in claim 14 is characterized in that, described correction volume is an actual volume, and described actual volume is represented based on the volume required amount of liquid of regulating in described most advanced and sophisticated retainer.
21, device as claimed in claim 14, it is characterized in that, described correction volume is a regulating error, and described regulating error is represented the poor of volume required and actual volume, and described actual volume representative is based on the volume required amount of liquid of regulating in described most advanced and sophisticated retainer.
22, device as claimed in claim 21 is characterized in that, described volume selector also is configured to allow the user to select new volume, and wherein said new volume comprises regulating error.
23, device as claimed in claim 21 is characterized in that, described display also is configured to high/low index is shown to the user of pipette, and described high/low index shows that regulating error is positive or negative.
24, device as claimed in claim 14 is characterized in that, described volume characterization is a form, and described form comprises:
A plurality of data points, wherein each data point comprises:
Calibrated volumes data point, wherein said calibrated volumes data point are represented the liquid volume that will regulate, and described calibrated volumes data point is selected as the part calibration process of pipette; And
Correction volume, wherein said correction volume are illustrated in the amount of liquid of regulating in the described most advanced and sophisticated retainer at calibrated volumes data point place.
25, device as claimed in claim 14 is characterized in that, described volume characterization is a form, and described form comprises:
A plurality of data points, wherein each data point comprises:
Calibrated volumes data point, wherein said calibrated volumes data point are represented the liquid volume that will regulate, and described calibrated volumes data point is selected as the part calibration process of pipette; And
Correction volume, wherein said correction volume are represented the poor of described calibrated volumes data point and actual volume, and wherein said actual volume is illustrated in described calibrated volumes data point place
The amount of liquid of regulating in the described most advanced and sophisticated retainer.
26, device as claimed in claim 14 is characterized in that, described volume characterization is an equation.
Applications Claiming Priority (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0313921 | 2003-11-27 | ||
| FR0313920A FR2862888B1 (en) | 2003-11-27 | 2003-11-27 | METHOD FOR DISPLAYING A VALUE OF A VOLUME OF A LIQUID SAMPLE TO BE TAKEN WITH A PIPETTE, WITH IMPROVED PRECISION |
| FR0313920 | 2003-11-27 | ||
| FR0402433 | 2004-03-09 | ||
| FR0409442 | 2004-09-07 | ||
| FR0409443 | 2004-09-07 | ||
| FR0409438 | 2004-09-07 | ||
| US10/944,532 | 2004-09-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1956786A true CN1956786A (en) | 2007-05-02 |
Family
ID=34566180
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004800351322A Pending CN1956786A (en) | 2003-11-27 | 2004-11-24 | System and method for accurate measuring volume of liquid in a pipette |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN1956786A (en) |
| FR (1) | FR2862888B1 (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102527457A (en) * | 2011-12-12 | 2012-07-04 | 浙江清华长三角研究院萧山生物工程中心 | Correction method for multi-channel electronic liquid-moving machine |
| CN103249487A (en) * | 2010-10-04 | 2013-08-14 | 埃佩多夫股份公司 | Electronic pipette |
| CN104136125A (en) * | 2012-02-13 | 2014-11-05 | 吉尔松有限合伙公司 | Sampling pipette which detects the passage of the piston through a predetermined position |
| CN104289259A (en) * | 2014-03-29 | 2015-01-21 | 浙江清华长三角研究院萧山生物工程中心 | Pipet calibration method based on machine vision |
| CN105785061A (en) * | 2016-03-04 | 2016-07-20 | 桂林优利特医疗电子有限公司 | Sample adding amount correcting method and system |
| CN106660046A (en) * | 2014-06-10 | 2017-05-10 | 恩姆菲舍尔科技公司 | Pipette |
| CN106807464A (en) * | 2017-01-31 | 2017-06-09 | 佛山市顺德区罗恩科学仪器有限公司 | Without menu mode pipettor and its pipetting method |
| CN107008515A (en) * | 2017-05-05 | 2017-08-04 | 盐城市华鸥实业有限公司 | A kind of laboratory reextraction separatory funnel |
| CN108241068A (en) * | 2016-12-27 | 2018-07-03 | 中国科学院微生物研究所 | A kind of automation micro-volume liquid relief and distributor and its application method |
| WO2018201701A1 (en) * | 2017-05-05 | 2018-11-08 | 盐城市华鸥实业有限公司 | Laboratory secondary extraction separatory funnel |
| CN109556919A (en) * | 2017-09-26 | 2019-04-02 | 深圳市新产业生物医学工程股份有限公司 | Liquid-transfering device and its liquid relief control method |
| CN110139715A (en) * | 2016-11-14 | 2019-08-16 | 艾卡工厂有限及两合公司 | Pipette and the method for waiting for liquid relief dosage for adjusting pipette |
| CN110300625A (en) * | 2016-12-21 | 2019-10-01 | 拜耳制药股份公司 | Measuring equipment |
| CN110465341A (en) * | 2016-06-15 | 2019-11-19 | 汉密尔顿公司 | Liquid-transfering device, pipette tip connector and pipette tip: device and method |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007126908A2 (en) | 2006-03-31 | 2007-11-08 | Artel, Inc. | Air displacement liquid delivery system and related method |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5187990A (en) * | 1984-02-16 | 1993-02-23 | Rainin Instrument Co., Inc. | Method for dispensing liquids with a pipette with compensation for air pressure and surface tension |
| US4586546A (en) * | 1984-10-23 | 1986-05-06 | Cetus Corporation | Liquid handling device and method |
| US5024109A (en) * | 1990-02-08 | 1991-06-18 | Medical Laboratory Automation, Inc. | Method and apparatus for performing hydrostatic correction in a pipette |
| DE4209620C1 (en) * | 1992-03-25 | 1993-12-16 | Eppendorf Geraetebau Netheler | Method for correcting the volume error ïV in a pipetting system |
| FR2807343B1 (en) * | 2000-04-07 | 2002-12-06 | Gilson Sa | COLLECTION PIPETTE PROVIDED WITH MEANS FOR ADJUSTING THE VOLUME TO BE COLLECTED |
| DE10118875C1 (en) * | 2001-04-18 | 2002-09-12 | Eppendorf Ag | Process for the controlled dosing of liquids using a displacement unit to push a gas pad used in pipettes comprises using the pressure measured in the gas pad at a point of complete release of the liquid volume |
-
2003
- 2003-11-27 FR FR0313920A patent/FR2862888B1/en not_active Expired - Fee Related
-
2004
- 2004-11-24 CN CNA2004800351322A patent/CN1956786A/en active Pending
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103249487A (en) * | 2010-10-04 | 2013-08-14 | 埃佩多夫股份公司 | Electronic pipette |
| CN103249487B (en) * | 2010-10-04 | 2016-02-17 | 埃佩多夫股份公司 | Electronic liquid trasferring device |
| CN102527457A (en) * | 2011-12-12 | 2012-07-04 | 浙江清华长三角研究院萧山生物工程中心 | Correction method for multi-channel electronic liquid-moving machine |
| CN104136125A (en) * | 2012-02-13 | 2014-11-05 | 吉尔松有限合伙公司 | Sampling pipette which detects the passage of the piston through a predetermined position |
| CN104136125B (en) * | 2012-02-13 | 2016-08-31 | 吉尔松有限合伙公司 | The detection piston sampling pipet passed through through precalculated position |
| CN104289259A (en) * | 2014-03-29 | 2015-01-21 | 浙江清华长三角研究院萧山生物工程中心 | Pipet calibration method based on machine vision |
| US10112192B2 (en) | 2014-06-10 | 2018-10-30 | Thermo Fisher Scientific Oy | Pipette comprising imaging device element |
| CN106660046A (en) * | 2014-06-10 | 2017-05-10 | 恩姆菲舍尔科技公司 | Pipette |
| CN106660046B (en) * | 2014-06-10 | 2019-09-13 | 恩姆菲舍尔科技公司 | Pipette |
| US10350589B2 (en) | 2014-06-10 | 2019-07-16 | Thermo Fisher Scientific Oy | Pipette comprising imaging device element |
| CN105785061A (en) * | 2016-03-04 | 2016-07-20 | 桂林优利特医疗电子有限公司 | Sample adding amount correcting method and system |
| CN105785061B (en) * | 2016-03-04 | 2017-11-17 | 桂林优利特医疗电子有限公司 | The bearing calibration of sample-adding amount and system |
| CN110465341A (en) * | 2016-06-15 | 2019-11-19 | 汉密尔顿公司 | Liquid-transfering device, pipette tip connector and pipette tip: device and method |
| CN110139715A (en) * | 2016-11-14 | 2019-08-16 | 艾卡工厂有限及两合公司 | Pipette and the method for waiting for liquid relief dosage for adjusting pipette |
| CN110139715B (en) * | 2016-11-14 | 2021-10-12 | 艾卡工厂有限及两合公司 | Pipette and method for adjusting the amount of liquid to be dispensed by a pipette |
| CN110300625A (en) * | 2016-12-21 | 2019-10-01 | 拜耳制药股份公司 | Measuring equipment |
| CN110300625B (en) * | 2016-12-21 | 2021-09-28 | 拜耳制药股份公司 | Metering device |
| CN108241068A (en) * | 2016-12-27 | 2018-07-03 | 中国科学院微生物研究所 | A kind of automation micro-volume liquid relief and distributor and its application method |
| CN106807464A (en) * | 2017-01-31 | 2017-06-09 | 佛山市顺德区罗恩科学仪器有限公司 | Without menu mode pipettor and its pipetting method |
| WO2018201701A1 (en) * | 2017-05-05 | 2018-11-08 | 盐城市华鸥实业有限公司 | Laboratory secondary extraction separatory funnel |
| CN107008515A (en) * | 2017-05-05 | 2017-08-04 | 盐城市华鸥实业有限公司 | A kind of laboratory reextraction separatory funnel |
| CN109556919A (en) * | 2017-09-26 | 2019-04-02 | 深圳市新产业生物医学工程股份有限公司 | Liquid-transfering device and its liquid relief control method |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2862888A1 (en) | 2005-06-03 |
| FR2862888B1 (en) | 2006-07-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1956786A (en) | System and method for accurate measuring volume of liquid in a pipette | |
| US20080271514A1 (en) | System and Method for Precise Liquid Measurement in a Liquid Sampling Pipette | |
| CN101144766B (en) | Full-automatic microhardness measuring system | |
| Friedman et al. | Capillary tube resistive thermal cycling | |
| CN103421688B (en) | Polymerase chain reaction device | |
| US20090055131A1 (en) | Automatic recording volume measurement apparatus | |
| CN1925917A (en) | Calibration pipette | |
| WO2012063043A1 (en) | Rheometer standardisation | |
| AU2004294757A1 (en) | Hand-held pipette for taking a liquid sample without temperature drift | |
| Krige et al. | Design and implementation of a low cost bio-printer modification, allowing for switching between plastic and gel extrusion | |
| CN200979419Y (en) | A multi-channel gas parameter measuring instrument | |
| US20160236189A1 (en) | Method for accurately calibrating discharge volume of pipette, and apparatus therefor | |
| CN109100502A (en) | A kind of full-automatic immunoblotting instrument | |
| Han et al. | Fabrication and thermal performance of a polymer-based flexible oscillating heat pipe via 3D printing technology | |
| CN1256415A (en) | Chip testing instrument with micro probe array | |
| CN104280559A (en) | Labonchip detector | |
| RU2353426C2 (en) | Method and system for accurate measurement of liquid in pipette for liquid sampling | |
| CN2840033Y (en) | High precision temperature control micro-chromatographic column box | |
| Mironov et al. | An experimental device for studying the 3D cryoprinting processes | |
| Meng et al. | Effect of surface tension on filling flow of carboxymethyl cellulose solution in microchannel | |
| CN103278663A (en) | Device and method for using both single molecular force spectroscope and magnetic tweezers on basis of glass micro needle | |
| Daoura et al. | Precise automated control of fluid volumes inside glass capillaries | |
| CN206488981U (en) | Micro constant volume nitrogen evaporator | |
| CN209663291U (en) | A kind of townhouse accurate pipettor | |
| CN203249937U (en) | A glass-micro-needle based apparatus integrating single-molecule force spectroscopy and magnetic tweezers |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |