CN102527457A - Correction method for multi-channel electronic liquid-moving machine - Google Patents

Abstract

The invention relates to a correction method for a multi-channel electronic liquid-moving machine. 50 percent of the maximum measuring range of the multi-channel electronic liquid-moving machine is taken as a measurement amount, so that more than three times of liquid-moving data of each channel are measured respectively, and difference of the channels is calculated statistically; the difference of the channels is subjected to variance inspection under the condition that alpha is equal to 0.05, so that the data of the channels is consistent; then a correction data constant 2''(In/2) of a middle single channel is used for correction; and the equation of the configuration volume=the constant 1*a set volume+the constant 2''(In/2) is met. The correction method has the beneficial effects that: proper correction is performed according to transverse error of the multi-channel electronic liquid-moving machine, so that the accuracy of each channel is guaranteed to the maximum extent; and furthermore, due to correction by adopting the technical scheme, only one more step of inspecting the consistency of the channels of the multi-channel electronic liquid-moving machine is increased than a single-channel electronic liquid-moving machine.

Description

A kind of bearing calibration that is used for the multiple tracks electronic liquid trasferring device

Technical field

The present invention relates to the electronic liquid trasferring device field, especially a kind of bearing calibration that is used for the multiple tracks electronic liquid trasferring device.

Background technology

The multiple tracks electronic liquid trasferring device is used for shifting in batches accurately the liquid sample or the reagent of certain volume, generally include the electronic section of compositions such as control button, display, stepper motor, controlling organization, battery and by piston set, sleeve, shirk the mechanical part that the supporting construction of sucker structure and electronic section is formed.Through the control of electronic section, piston set is utilized the air displacement principle, changes the air containment in the plunger shaft thereby drive reciprocating motion of the pistons through the control circuit drive stepping motor, reaches the soakage or the discharge rate of accurate control liquid.

The single track electronic liquid trasferring device is in order to reach the standard of accurately moving liquid, and it has calibration function usually to allow the setting piston stroke.The calibration steps of existing electronic liquid trasferring device is: under room temperature (20～25 ℃), weigh up the weight by the distilled water (or other liquid) with the pipette preparation that shows volume.The execution of calibration is based on following hypothesis: setting volume and dose volume is linear correlation.Satisfy dose volume=constant 1 * setting volume+constant 2 (I), wherein constant 1 is an ascent, and constant 2 is correction coefficient.

The calibration steps of existing multiple tracks electronic liquid trasferring device is proofreaied and correct separately on a plurality of piston channels of correspondence based on the bearing calibration of single track electronic liquid trasferring device, obtains corresponding constant 2 (I ₁～I _n), get mean constant 2 ' (I ').Satisfied configuration volume=constant 1 * setting volume+constant 2 ' (I ').

But existing bearing calibration needs respectively each passage to be proofreaied and correct test, and trimming process needs the manpower of labor.

Summary of the invention

The present invention will solve the shortcoming of above-mentioned prior art, provides a kind of step the simple bearing calibration that is used for the multiple tracks electronic liquid trasferring device.

The present invention solves the technical scheme that its technical problem adopts:

Get 50% of multiple tracks electronic liquid trasferring device maximum range and be measured quantity; Measure each passage respectively and move the liquid data more than three times, 50% is default value, is positioned at the middle of piston stroke; This point accurately for whole accurately important to pipettor; Carry out each passage difference of variance test with α=0.05, confirm that each channel data is consistent, after get the correction data constant 2 of single passage placed in the middle " (I _N/2) proofread and correct, the control push rod of multiple tracks electronic liquid trasferring device is in the middle, and the data of single passage placed in the middle in theory are the most accurate, and n is generally even number, and single passage then placed in the middle is n/2 passage and n/2+1 passage, satisfies:

Configuration volume=constant 1 * setting volume+constant 2 " (I _N/2).

As preferably, each channel data contrast is the contrast of the passage more than 2 or 2.

As preferably, the consistent statistical method of each channel data of said calculating is following:

Channel group number=a, number of times=b, x _IjBe that the i passage moves liquid measure for the j time, obtain population variance V, intra-class variance V _W, between-group variance V _B, wherein:

Population variance $V=\mathrm{\Σ}{(x_{\mathrm{Ij}}-\stackrel{\‾}{x})}^2;$

Intra-class variance $V_w=\mathrm{\Σ}{(x_{\mathrm{Ij}}-\stackrel{\‾}{x_i})}^2;$

Between-group variance $V_B=b\×\mathrm{\Σ}{(\stackrel{\‾}{x_i}-\stackrel{\‾}{x})}^2;$

Mean squares within group is poor $S_W^2=\frac{V_W}{\mathrm{Ab}-a};$

Mean squares between groups is poor $S_B^2=\frac{V_B}{a-1};$

Suppose H ₀: each channel data is consistent, H ₁: each channel data is not quite identical;

Utilization F statistic is carried out variance test:

$F=\frac{V_B/(a-1)}{V_W/(\mathrm{ab}-a)}=\frac{S_B^2}{S_W^2};$

By given significance α=0.05, with free degree v ₁=a-1, v ₂=ab-a looks into F dividing value table (variance analysis is used), gets F _α(a-1, if ab-a) value is F＜F _α, then accept H ₀, think that each passage average is consistent; If F>=F _α, then accept H ₁, think that each passage average is not quite identical.

Inventing useful effect is: the lateral error that the present invention exists according to the multiple tracks electronic liquid trasferring device; Suitably proofread and correct; Guaranteed the accuracy of each passage to greatest extent; Proofread and correct through this technical scheme, the correction of multiple tracks electronic liquid trasferring device only increases the step of each passage consistency check than single track electronic liquid trasferring device.

The specific embodiment

Embodiment 1: get 50% of multiple tracks electronic liquid trasferring device maximum range and be measured quantity, measure eight passages respectively and move liquid measure for five times, write down like following table:

According to the above numerical value that obtains, group number a=8, number of times b=6,

Population variance V=0.12156,

Intra-class variance V _w=0.10952,

Between-group variance V _B=0.01204,

Mean squares within group is poor $S_W^2=0.00342,$

Mean squares between groups is poor $S_B^2=0.00172,$

$F=\frac{V_B/(a-1)}{V_W/(\mathrm{ab}-a)}=\frac{S_B^2}{S_W^2}=0.50256,$

v ₁＝a-1＝7，

v ₂＝ab-a＝32，

α＝0.05，

F _α(7，32)＝2.33＜F＝0.50256

So, think that eight passage numerical value are consistent.Get the single passage placed in the middle correction data constant 2 of passage 4 or passage 5 just " (In/2) proofread and correct, satisfy: configuration volume=constant 1 * setting volume+constant 2 " (I _N/2).

Embodiment 2: according to the data of embodiment 1, contrast the single calibration point data consistency of the 1st passage and last passage, confirm the stability of multi-channel structure, then through the single channel correction data of the 4th passage whole pipettor proofreaied and correct.

Embodiment 3: comparative example 1 data; Contrast the single calibration point data consistency of the 1st passage, the 4th passage and last passage; Confirm the stability of multi-channel structure, then whole pipettor proofreaied and correct through the single channel correction data of the 5th passage.

Except that the foregoing description, the present invention can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of requirement of the present invention.

Claims (2)

1. bearing calibration that is used for multiple tracks electro-hydraulic device is characterized in that:

1) under 20～25 ℃ room temperature, weigh up weight by known density liquid with the pipette preparation that shows volume, the execution of calibration is based on following hypothesis, and setting volume and dose volume is linear correlation, satisfies:

Dose volume=constant 1 * setting volume+constant 2 (I)

Wherein constant 1 is an ascent, and constant 2 is correction coefficient;

2) get 50% of multiple tracks electronic liquid trasferring device maximum range and be measured quantity; Measure each passage respectively and move the liquid data more than three times, carry out each passage difference of variance test, confirm that each channel data is consistent with α=0.05; After get single passage placed in the middle, according to 1) method draw correction data constant 2 " (I _N/2) proofread and correct, satisfy:

Configuration volume=constant 1 * setting volume+constant 2 " (I _N/2)

2. the bearing calibration that is used for multiple tracks electro-hydraulic device according to claim 1 is characterized in that: each channel data contrast is the contrast of the passage more than 2 or 2.