CN109556919A - Liquid-transfering device and its liquid relief control method - Google Patents
Liquid-transfering device and its liquid relief control method Download PDFInfo
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- CN109556919A CN109556919A CN201710882677.6A CN201710882677A CN109556919A CN 109556919 A CN109556919 A CN 109556919A CN 201710882677 A CN201710882677 A CN 201710882677A CN 109556919 A CN109556919 A CN 109556919A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
Abstract
The present invention relates to a kind of liquid-transfering device and its liquid relief methods, including setting N number of liquid relief control amount, N number of practical liquid relief amount is set, N number of practical liquid relief amount section is obtained, which section that practical liquid relief value needed for this liquid relief is fallen into N number of practical liquid relief amount section determined；According to the practical liquid relief amount and two corresponding liquid relief control amounts of the two of the section fallen into endpoints, section compensation is carried out to the practical liquid relief value, and carry out environmental factor compensation, obtain liquid relief controlling value；The liquid-transfering device liquid relief is controlled according to the liquid relief controlling value.Influence of the viscosity of hysterisis error, outside environmental elements and different sample to inhaling sample, spitting sample amount is eliminated, makes practical suction sample, spit sample amount and demand is consistent substantially.Stability, repeatability of the liquid-transfering device in pipetting processes are improved, and then improves the detection reliability of vitro diagnostic test systems.
Description
Technical field
The present invention relates to the field of medical instrument technology, more particularly to a kind of liquid-transfering device and its liquid relief control method.
Background technique
Now, testing agency generally carries out quantitative analysis according to the test result of extracorporeal diagnostic instrument.Pneumatic/hydraulic liquid relief
Device has important influence to extracorporeal diagnostic instrument as adjacent components important in extracorporeal diagnostic instrument, performance.In body
Outer diagnostic field is realized reduction by using the pneumatic/hydraulic liquid-transfering device that can load disposable tip or prevents to carry dirty
The mode of dye rate has become a kind of trend.Sample-adding performance (stability, repeatability) direct shadow of pneumatic/hydraulic liquid-transfering device
The performance of entire extracorporeal diagnostic system is rung, according to matched closed reagent system requirements, pneumatic/hydraulic liquid-transfering device can be in difference
In the environment of, different magnitudes of drawing or spue, different types of liquid.But in the use process of pneumatic/hydraulic liquid-transfering device
In, it is practical to inhale sample, spit sample amount, and theoretical inhale, spit sample amount and have different.The mode for generally needing to be spat sample compensation with suction sample, makes
The practical suction sample of pneumatic/hydraulic liquid-transfering device spits sample amount and reaches requirement.
But in vitro in diagnostic instrments, pneumatic/hydraulic liquid-transfering device is combined due to internal piston by gear and screw rod
Mechanical structure is realized, can generate backhaul cumulative errors in use, for a long time using will lead to mechanical structure aging, and by the external world
It inhales sample and spits the influence of sample environment (air pressure, temperature, humidity, vibration etc.), lead to practical suctions, spit sample amount and theoretical suction, spit sample amount and have
Different, and be drawn, the sliminess of the liquid that spues (such as: water, serum) it is different, will affect practical suction, spit sample
The variation of amount and the theoretical difference value for inhaling, spitting sample amount.Furthermore under identical environment, the same type that sample spits sample same volume is inhaled
Liquid when, the difference of practical uptake and theoretical uptake when inhaling sample spits sample amount and theory spits sample amount with practical when spitting sample
Difference be different, i.e., single compensated curve can not meet simultaneously inhale sample and spit sample simultaneously it is accurate.
Summary of the invention
Based on this, it is necessary to it is theoretical in pipetting processes to spit sample compensated curve for the suction sample that traditional liquid-transfering device uses
Liquid relief value and practical liquid relief value have differences, will lead to the problem of liquid relief inaccuracy, provide a kind of liquid relief precision that can be improved
Liquid-transfering device and its liquid relief control method.
A kind of liquid relief control method of liquid-transfering device, comprising:
N number of liquid relief control amount is set, N number of liquid relief control amount is arranged successively by numerical values recited, and the N is natural number；
N number of practical liquid relief amount is set, N number of practical liquid relief amount section, N number of practical liquid relief amount and N number of shifting are obtained
Liquid control amount corresponds；
Which section that practical liquid relief value needed for this liquid relief is fallen into N number of practical liquid relief amount section determined；
According to the practical liquid relief amount and two corresponding liquid relief control amounts of the two of the section fallen into endpoints, to the reality
Border liquid relief value carries out section compensation, and carries out environmental factor compensation, obtains liquid relief controlling value；
The liquid-transfering device liquid relief is controlled according to the liquid relief controlling value.
The environmental factor compensation includes kind of liquid compensation, compressing liquid velocity compensation, inhales in one of the embodiments,
Spit liquid mode compensate, ambient humidity compensation, ambient temperature compensation, ambient pressure compensation at least one of.
N number of practical liquid relief amount in one of the embodiments, is the control liquid-transfering device respectively according to the N
A liquid relief control amount carries out liquid relief, and then measurement obtains.
The value of the N is greater than or equal to 2 in one of the embodiments,.
N number of liquid relief control amount is summarized according to the actual demand of the liquid-transfering device in one of the embodiments,
N number of common liquid relief amount out.
It is described in one of the embodiments, to include: to the practical liquid relief value progress section compensation
According to N number of liquid relief control amount and N number of practical liquid relief amount, piecewise interval letter is established with linear equation in two unknowns
Number.
The compensated practical liquid relief value in section in one of the embodiments,
Wherein, X' is practical liquid relief value needed for this liquid relief, XnAnd Xn+1In the N number of liquid relief control amount respectively set
N-th of liquid relief control amount value and (n+1)th liquid relief control amount value, Xn' and Xn+1' it is respectively the N number of practical liquid relief set
The value of n-th of practical liquid relief amount in amount and the value of (n+1)th practical liquid relief amount.
Obtaining liquid relief controlling value described in one of the embodiments, includes:
It determines affiliated section of the practical liquid relief value needed in N number of practical liquid relief amount of setting, that is, finds the reality of needs
Border liquid relief value between the value of n-th of practical liquid relief amount and the value of (n+1)th practical liquid relief amount between, and determine n value；
The value of n-th of liquid relief control amount and the value of (n+1)th liquid relief control amount are determined according to the value of the n；
By the practical liquid relief value of the needs, the value of n-th practical liquid relief amount, the value of (n+1)th practical liquid relief amount, n-th
The value of a liquid relief control amount and the value of (n+1)th liquid relief control amount substitute into the pass of the liquid relief control amount Yu the practical liquid relief amount
It is the liquid relief controlling value needed in formula.
In one of the embodiments, the numerical values recited of N number of liquid relief control amount and N number of practical liquid relief amount be incremented by or
Successively decrease.
A kind of liquid-transfering device, including controller, the controller is for executing above-mentioned method to carry out liquid relief control.
Above-mentioned liquid-transfering device and its liquid relief control method, by setting N number of liquid relief control amount and N number of practical liquid relief amount, and
N number of practical liquid relief amount section is obtained, determines which in N number of practical liquid relief amount section be practical liquid relief value needed for this liquid relief fall into
A section；According to the practical liquid relief amount and two corresponding liquid relief control amounts of the two of the section fallen into endpoints, to practical liquid relief
Value carries out section compensation, and carries out environmental factor compensation, obtains liquid relief controlling value.Eliminate hysterisis error, outside environmental elements
Influence with the viscosityes of different samples to inhaling sample, spitting sample amount makes practical suction sample, spits sample amount and demand is consistent substantially.
Stability, repeatability of the liquid-transfering device in pipetting processes are improved, and then the detection for improving vitro diagnostic test systems can
By property.
Detailed description of the invention
Fig. 1 is the flow chart of the liquid relief control method of liquid-transfering device in one embodiment.
Specific embodiment
To facilitate the understanding of the present invention, a more comprehensive description of the invention is given in the following sections with reference to the relevant attached drawings.In attached drawing
Give presently preferred embodiments of the present invention.But the invention can be realized in many different forms, however it is not limited to this paper institute
The embodiment of description.On the contrary, purpose of providing these embodiments is keeps the understanding to the disclosure more thorough
Comprehensively.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that the limitation present invention.It is appreciated that term " first " used in the present invention, " second "
Etc. can be used to describe various elements herein, but these elements should not be limited by these terms.These terms are only used to by first
A element and another element are distinguished.Term as used herein "and/or" includes one or more relevant listed items
Any and all combinations.
A kind of liquid relief control method of liquid-transfering device presented below, liquid relief can be imbibition, be also possible to spit liquid.By right
Liquid-transfering device makes practical suction sample, spits sample amount and demand is kept substantially inhaling sample and spit sample during carries out curve compensation
Unanimously.As shown in fig.1, this approach includes the following steps S110~S150.
S110: setting N number of liquid relief control amount, and N number of liquid relief control amount is arranged successively by numerical values recited, and the N is certainly
So number.
Specifically, N number of liquid relief control amount is set according to actual needs, and N number of liquid relief control amount is according to the liquid relief
N number of common liquid relief amount that the actual demand of device sums up.The liquid relief control amount indicates that control liquid-transfering device carries out liquid relief
Controlling value, the liquid-transfering device can be pneumatically or hydraulically liquid-transfering device.Wherein the value of N can be according to during in real work
Demand is configured, and N is natural number.After setting N number of liquid relief control amount, this N number of liquid relief control amount according to numerical values recited according to
Secondary arrangement.Optionally, the numerical values recited of N number of liquid relief control amount can be increasing or decreasing.
Further, the value of the N is greater than or equal to 2.Such as: 8 common liquid relief control amounts of setting are set to
X1, X2..., X8, this 8 liquid relief control amounts are according to X1~X8Number order arranged, and ascending number is corresponding
The numerical value of control amount is incremented by successively.And the accuracy of liquid relief controlling value and the value size of N are positively correlated, and the numerical value of N is bigger,
Obtained liquid relief controlling value is more accurate, makes practical suction sample, spits sample amount and the difference of demand is smaller.
S120: the N number of practical liquid relief amount of setting obtains N number of practical liquid relief amount section, N number of practical liquid relief amount and the N
A liquid relief control amount corresponds.
Specifically, it is practical after practical liquid relief amount indicates that liquid-transfering device carries out liquid relief according to the liquid relief control amount of above-mentioned setting
The amount of liquid of transfer.Therefore, each liquid relief control amount is corresponding with a practical liquid relief amount, according to N number of liquid relief control of above-mentioned setting
Amount processed sets N number of practical liquid relief amount, and N number of practical liquid relief amount is by controlling the liquid-transfering device respectively according to N number of shifting
Liquid control amount carries out liquid relief, and then measurement obtains, and the value of practical liquid relief amount and the value of liquid relief control amount have one-to-one pass
System.After setting N number of practical liquid relief amount, the practical liquid relief amount of adjacent two constitutes practical liquid relief amount section, therefore obtains N number of reality
Border liquid relief amount section.
It is understood that above-mentioned practical liquid relief amount, according to different liquid-transfering devices, the numerical value of setting is different.
S130: which area that practical liquid relief value needed for this liquid relief is fallen into N number of practical liquid relief amount section determined
Between.
Specifically, practical liquid relief value needed for this liquid relief is obtained, which is that liquid-transfering device is being inhaled sample or spat
Target during sample inhales sample or spits sample amount.The N number of practical liquid relief amount set in the practical liquid relief value and S120 is compared,
And determine the practical liquid relief value falls into which section in N number of practical liquid relief amount section.
S140: according to the practical liquid relief amount and two corresponding liquid relief control amounts of the two of the section fallen into endpoints, to institute
It states practical liquid relief value and carries out section compensation, and carry out environmental factor compensation, obtain liquid relief controlling value.
Specifically, the mode of section compensation can be with are as follows: according to N number of liquid relief control amount and N number of practical liquid relief amount, utilization
Linear equation in two unknowns establishes piecewise interval function.Environmental factor compensation include kind of liquid compensation, compressing liquid velocity compensation,
Compressing liquid mode (such as liquid-transfering device is pneumatic or surges) compensation, ambient humidity compensation, ambient temperature compensation, ambient pressure
At least one of compensation.
The compensation of above-mentioned section is illustrated, according to N number of liquid relief control amount and N number of practical liquid relief amount, is used
The piecewise interval function that linear equation in two unknowns is established are as follows:Wherein, X is the shifting needed
Liquid controlling value, X' are the practical liquid relief value needed, XnAnd Xn+1N-th of the liquid relief control in N number of liquid relief control amount respectively set
The value of the value of amount processed and (n+1)th liquid relief control amount, Xn' and Xn+1' be respectively setting N number of practical liquid relief amount in n-th reality
The value of the value of border liquid relief amount and (n+1)th practical liquid relief amount.It is understood that the liquid relief control amount and practical liquid relief amount
Relational expression carries out corresponding setting according to different environmental factors.
The compensation of above-mentioned environmental factor is illustrated, kind of liquid is compensated, by liquid-transfering device using identical
Liquid relief controlling value come the liquid for different viscosityes of drawing or spue, according to it is practical inhale sample or spit sample amount construct different liquid
Type compensated curve is to meet the actual use demand of liquid-transfering device.Similarly, it is formed by liquid-transfering device in different altitude height
Liquid relief is carried out using identical liquid relief controlling value under conditions of different ambient pressures, to obtain ambient pressure compensated curve.Similarly
It is bent that compressing liquid speed degree compensated curve, compressing liquid mode compensated curve, ambient humidity compensated curve, ambient temperature compensation can be obtained
Line etc..
In one embodiment, firstly, belonging to determining the practical liquid relief value needed in N number of practical liquid relief amount of setting
Section, that is, find the practical liquid relief values of needs between n-th of practical liquid relief amount value and (n+1)th practical liquid relief amount value it
Between, and determine the value of n.Secondly, determining the value and (n+1)th liquid relief control amount of n-th of liquid relief control amount according to the value of the n
Value.Finally, by the practical liquid relief value of the needs, the value of n-th practical liquid relief amount, the value of (n+1)th practical liquid relief amount, n-th
The value of a liquid relief control amount and the value of (n+1)th liquid relief control amount substitute into the pass of the liquid relief control amount Yu the practical liquid relief amount
It is the liquid relief controlling value needed in formula.Optionally, practical liquid relief value can also be determined according to liquid relief controlling value.
S150: the liquid-transfering device liquid relief is controlled according to the liquid relief controlling value.
Specifically, in vitro in the practical application of diagnostic instrments, practical liquid relief amount determines the stabilization of whole system, therefore
It needs to be determined that liquid relief controlling value control liquid-transfering device meet demand.
In one embodiment, a kind of suction sample curve compensation method of liquid-transfering device is provided, is matched according to extracorporeal diagnostic instrument
The closed reagent system requirements of set sum up 8 common suction amounts, are set as X1, X2..., X8.It should according to extracorporeal diagnostic system setting
The controlling value of liquid-transfering device are as follows: X1=50, X2=100, X3=250, X4=500, X5=750, X6=1250, X7=2000, X8=
3000.If the practical suction amount of 8 controlling values is X1', X2', X3', X4', X5', X6', X7', X8'.Wherein, X1' correspond to X1, X2'
Corresponding X2, X3' correspond to X3, X4' correspond to X4, X5' correspond to X5, X6' correspond to X6, X7' correspond to X7, X8' correspond to X8.Meanwhile setting X1'=
34, X2'=88, X3'=241, X4'=493, X5'=746, X6'=1248, X7'=2057, X8'=3115.Pass through this 8 realities
Border liquid relief amount, 8 obtained practical liquid relief amount sections are as follows: (0,34), (34,88), (88,241), (241,493), (493,
746), (746,1248), (1248,2057), (2057,3115).In other embodiments, the above Xn' according to different liquid reliefs
Device, numerical value are different.Under the conditions of different suction amounts, XnWith Xn' (N=0,1 ..., 8) difference be different.So needing
Recruiting curve is set according to different sample-adding amounts.
Further, when practical sampling amount is X', as X' ∈ (Xn', Xn+1') n=(0,1,2 ..., 8) when, it is corresponding
Controlling value is to set X, then has:
Then:
Wherein, X is the suction sample controlling value needed, and X' be the practical suction sample value of needs, when needing liquid-transfering device to go practical suction
It is then X to the controlling value of liquid-transfering device when taking the liquid of X' volume.
In one embodiment, when needing practical absorption liquid volume is 115 μ l, then X'=1150*0.1 μ l is known,
Meanwhile 1150 ∈ (746,1248) sections, i.e. this practical liquid relief amount 1150 fall into practical liquid relief amount section (746,1248), then
Xn'=746, Xn+1'=1248, n=5.X is determined by n=5nAnd Xn+1Respectively X5And X6, so Xn=750, Xn+1=1250.
According toObtain liquid relief controlling value X are as follows:
When the controlling value of liquid-transfering device is 1152, the practical suction amount of liquid-transfering device is very close to 1150*0.1 μ l.
As above, pneumatic (surging) liquid-transfering device suction sample curve compensation can be according to liquid, the difference for drawing different viscosityes
Height above sea level row is at different ambient pressures, different compressing liquid speed degree, different imbibition modes, varying environment humidity, varying environment temperature
Degree etc. constructs different curves to meet the actual use demand of liquid-transfering device, keeps the amount of liquid for sucking liquid-transfering device more acurrate.
In another embodiment, provide a kind of liquid-transfering device spits sample curve compensation method, and it is bent to inhale sample with liquid-transfering device
Line compensation method principle is identical.According to the matched closed reagent system requirements of extracorporeal diagnostic instrument sum up 8 it is common spit sample amount,
It is set as Y1, Y2..., Y8.This 8 values represent and control the controlling value that pneumatic (surging) liquid-transfering device spits sample.If the reality of 8 controlling values
It is Y that sample amount is spat on border1', Y2', Y3', Y4', Y5', Y6', Y7', Y8'.Wherein, Y1' correspond to Y1, Y2' correspond to Y2, Y3' correspond to Y3, Y4' right
Answer Y4, Y5' correspond to Y5, Y6' correspond to Y6, Y7' correspond to Y7, Y8' correspond to Y8。
It is Y' when actually spitting sample amount, as Y' ∈ (Yn', Yn+1') n=(0,1,2 ..., 8) when, corresponding controlling value is set as
Y then has:
Then:
When pneumatic liquid-transfering device is when to spit sample controlling value be Y, pneumatic liquid-transfering device actually spits sample amount very close to Y'.Together
Reason, liquid-transfering device spit sample curve compensation can according to the liquid for drawing different viscosityes, different altitude height row is at the different external worlds
Pressure, different compressing liquid speed degree, it is different spit liquid mode, varying environment humidity, varying environment temperature etc. construct different curves with
The actual use demand for meeting liquid-transfering device keeps the amount of liquid to spue from liquid-transfering device more acurrate.
Based on identical inventive concept, a kind of liquid-transfering device is provided, which includes controller, and the controller is used
In execute the various embodiments described above method to carry out liquid relief control.
Above-mentioned liquid-transfering device and its liquid relief control method, by setting N number of liquid relief control amount and N number of practical liquid relief amount, and
N number of practical liquid relief amount section is obtained, determines which in N number of practical liquid relief amount section be practical liquid relief value needed for this liquid relief fall into
A section；According to the practical liquid relief amount and two corresponding liquid relief control amounts of the two of the section fallen into endpoints, to practical liquid relief
Value carries out section compensation, and carries out environmental factor compensation, can obtain different compensated curves according to different actual environments, thus
Obtain liquid relief controlling value.The viscosity of hysterisis error, outside environmental elements and different samples is eliminated to the shadow inhaled sample, spit sample amount
It rings, makes practical suction sample, spits sample amount and demand is consistent substantially.Improve stability of the liquid-transfering device in pipetting processes,
Repeatability, and then improve the detection reliability of vitro diagnostic test systems.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of liquid relief control method of liquid-transfering device, comprising:
N number of liquid relief control amount is set, N number of liquid relief control amount is arranged successively by numerical values recited, and the N is natural number；
N number of practical liquid relief amount is set, N number of practical liquid relief amount section, N number of practical liquid relief amount and N number of liquid relief control are obtained
Amount processed corresponds；
Determine the practical liquid relief amount section that practical liquid relief value needed for this liquid relief is fallen into；
According to the practical liquid relief amount and two corresponding liquid relief control amounts of the two of the section fallen into endpoints, to the practical shifting
Liquid value carries out section compensation, and carries out environmental factor compensation, obtains liquid relief controlling value；
The liquid-transfering device liquid relief is controlled according to the liquid relief controlling value.
2. the method according to claim 1, wherein environmental factor compensation includes kind of liquid compensation, inhales
Spit liquid velocity compensation, compressing liquid mode compensates, ambient humidity compensation, ambient temperature compensation, ambient pressure compensation at least one
Kind.
3. the method according to claim 1, wherein N number of practical liquid relief amount, is the control liquid-transfering device
Measure after liquid relief obtaining according to the N number of liquid relief control amount respectively.
4. the method according to claim 1, wherein the value of the N is greater than or equal to 2.
5. the method according to claim 1, wherein N number of liquid relief control amount is according to the liquid-transfering device
N number of common liquid relief amount for summing up of actual demand.
6. the method according to claim 1, wherein described carry out section compensation packet to the practical liquid relief value
It includes:
According to N number of liquid relief control amount and N number of practical liquid relief amount, piecewise interval function is established with linear equation in two unknowns.
7. according to the method described in claim 6, it is characterized in that, the compensated practical liquid relief value in section
Wherein, X' is practical liquid relief value needed for this liquid relief, XnAnd Xn+1N-th in N number of liquid relief control amount respectively set
The value of a liquid relief control amount and the value of (n+1)th liquid relief control amount, Xn' and Xn+1' it is respectively in N number of practical liquid relief amount of setting
N-th of practical liquid relief amount value and (n+1)th practical liquid relief amount value.
8. the method according to the description of claim 7 is characterized in that the liquid relief controlling value that obtains includes:
It determines affiliated section of the practical liquid relief value needed in N number of practical liquid relief amount of setting, that is, finds the practical shifting of needs
Liquid value between the value of n-th of practical liquid relief amount and the value of (n+1)th practical liquid relief amount between, and determine n value；
The value of n-th of liquid relief control amount and the value of (n+1)th liquid relief control amount are determined according to the value of the n；
By the practical liquid relief value of the needs, the value of n-th practical liquid relief amount, the value of (n+1)th practical liquid relief amount, n-th of shifting
The value of the value of liquid control amount and (n+1)th liquid relief control amount substitutes into the relational expression of the liquid relief control amount Yu the practical liquid relief amount
In, the liquid relief controlling value that is needed.
9. liquid relief method according to claim 1, which is characterized in that N number of liquid relief control amount and N number of practical liquid relief amount
Numerical values recited be increasing or decreasing.
10. a kind of liquid-transfering device, which is characterized in that including controller, the controller is in execution according to claim 1-9
Described in any item methods are to carry out liquid relief control.
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Application publication date: 20190402 |