CN1950497A - Probiotic composition - Google Patents
Probiotic composition Download PDFInfo
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- CN1950497A CN1950497A CNA2005800138218A CN200580013821A CN1950497A CN 1950497 A CN1950497 A CN 1950497A CN A2005800138218 A CNA2005800138218 A CN A2005800138218A CN 200580013821 A CN200580013821 A CN 200580013821A CN 1950497 A CN1950497 A CN 1950497A
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- probiotic composition
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- 239000000203 mixture Substances 0.000 title claims abstract description 45
- 239000006041 probiotic Substances 0.000 title claims abstract description 45
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 45
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 45
- 235000013336 milk Nutrition 0.000 claims description 23
- 239000008267 milk Substances 0.000 claims description 23
- 210000004080 milk Anatomy 0.000 claims description 23
- 239000000049 pigment Substances 0.000 claims description 14
- 239000000989 food dye Substances 0.000 claims description 13
- 235000020183 skimmed milk Nutrition 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 45
- 244000005700 microbiome Species 0.000 abstract description 25
- 235000013305 food Nutrition 0.000 abstract description 13
- 239000007788 liquid Substances 0.000 abstract description 11
- 230000001766 physiological effect Effects 0.000 abstract 2
- 239000003086 colorant Substances 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 20
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 20
- 230000035611 feeding Effects 0.000 description 16
- 241000287828 Gallus gallus Species 0.000 description 14
- 235000013330 chicken meat Nutrition 0.000 description 14
- 239000000654 additive Substances 0.000 description 13
- 230000000996 additive effect Effects 0.000 description 13
- 230000000694 effects Effects 0.000 description 13
- 235000014655 lactic acid Nutrition 0.000 description 10
- 230000004083 survival effect Effects 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 238000000855 fermentation Methods 0.000 description 8
- 230000004151 fermentation Effects 0.000 description 8
- 230000007774 longterm Effects 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 230000007613 environmental effect Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 230000037396 body weight Effects 0.000 description 4
- 238000013016 damping Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 235000012041 food component Nutrition 0.000 description 3
- 239000005417 food ingredient Substances 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 239000013535 sea water Substances 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 241000370738 Chlorion Species 0.000 description 2
- 241000360771 Coreana Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- QWVMSYBGKWZIIE-RDFNRINOSA-N Flavochrome Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C1OC2(C)CCCC(C)(C)C2=C1)C=CC=C(/C)C=CC3C(=CCCC3(C)C)C QWVMSYBGKWZIIE-RDFNRINOSA-N 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000013527 degreasing agent Substances 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- QWVMSYBGKWZIIE-FZKBJVJCSA-N flavochrome Chemical compound O1C2(C)CCCC(C)(C)C2=CC1C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1C(C)=CCCC1(C)C QWVMSYBGKWZIIE-FZKBJVJCSA-N 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000000644 isotonic solution Substances 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- RECVMTHOQWMYFX-UHFFFAOYSA-N oxygen(1+) dihydride Chemical compound [OH2+] RECVMTHOQWMYFX-UHFFFAOYSA-N 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 239000001052 yellow pigment Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- E—FIXED CONSTRUCTIONS
- E04—BUILDING
- E04H—BUILDINGS OR LIKE STRUCTURES FOR PARTICULAR PURPOSES; SWIMMING OR SPLASH BATHS OR POOLS; MASTS; FENCING; TENTS OR CANOPIES, IN GENERAL
- E04H17/00—Fencing, e.g. fences, enclosures, corrals
- E04H17/14—Fences constructed of rigid elements, e.g. with additional wire fillings or with posts
- E04H17/1413—Post-and-rail fences, e.g. without vertical cross-members
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/40—Colouring or decolouring of foods
- A23L5/42—Addition of dyes or pigments, e.g. in combination with optical brighteners
-
- E—FIXED CONSTRUCTIONS
- E04—BUILDING
- E04H—BUILDINGS OR LIKE STRUCTURES FOR PARTICULAR PURPOSES; SWIMMING OR SPLASH BATHS OR POOLS; MASTS; FENCING; TENTS OR CANOPIES, IN GENERAL
- E04H17/00—Fencing, e.g. fences, enclosures, corrals
- E04H17/14—Fences constructed of rigid elements, e.g. with additional wire fillings or with posts
- E04H17/1413—Post-and-rail fences, e.g. without vertical cross-members
- E04H17/1447—Details of connections between rails and posts
- E04H17/1452—Details of connections between rails and posts the ends of the rails are fixed on the lateral sides of the posts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Engineering & Computer Science (AREA)
- Architecture (AREA)
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Civil Engineering (AREA)
- Structural Engineering (AREA)
- Mycology (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a probiotic composition which enables to maintain effectively physiological activity of functional microorganism contained therein for a long time. More par not ticularly, the present invention is directed to a probiotic composition comprising a colorant which maintains effectively physiological activity of a functional microorganism for a long time and increases the convenience for use by being mixed or melted easily in both liquid type and pulverulent type medium such as water, food or fodder, etc.
Description
Technical field
The present invention relates to a kind ofly can keep the wherein probiotic composition of the physiologically active of functional microorganism long-term effectively.More particularly about a kind of probiotic composition that contains pigment, this pigment can be kept the physiologically active of functional microorganism long-term effectively, and make that this probiotic composition is easier to be mixed with liquid state such as water, food and feed or powdery medium or dissolve in the above-mentioned medium, thereby improve the comfort level of using.
Background technology
Various microorganisms with physiologically active for example have and suppress harmful bacteria growing and promote the microorganism of intestines peristalsis by improving GI microecological balance, have been used as a kind of effective constituent of so-called probiotic bacterium.
Described " probiotic bacterium " thus be meant that those can be by improving GI microecological balance to going into or animal produces the living microorganism foodstuff additive of active effect.
Because various Effect of Environmental, being exposed to as the functional microbial of the effective constituent in the probiotic bacterium just can be dead immediately in the outside atmosphere.Therefore, between the preservation period of these living microorganism foodstuff additive, be necessary to reduce as far as possible of the influence of various environmental factorss to it.
Therefore, people have carried out research chronically to the method for keeping its physiologically active by the variation that reduces living microorganism foodstuff additive environmental factors during preservation as far as possible effectively.
Suppressing the during preservation dead method of microorganism in the prior art can be divided into following several: lyophilize and spraying drying make its dehydration (Korean patent No. 0137154,0148340 and 2002-0097237), micro encapsulation or it is wrapped by (Korean patent No. 2002-0069863 and 2003-0009268).
These traditional methods help the prolonged preservation of living microorganism foodstuff additive and it is mixed more easily with food.
Yet, need probiotic bacterium is soluble in water such as with above-mentioned living microorganism foodstuff additive feeding fish or bird the time, but such dissolving is difficult, even if this probiotic bacterium can be water-soluble, because the change of environment also can cause the death of microorganism immediately.
In other words, because the solubleness of probiotic bacterium in water of micro encapsulation or bag quilt is lower, so it is not suitable for being used for feeding fish and bird.And in case be dissolved in the water, environmental factors also can produce bigger influence to the exsiccant probiotic bacterium, for example the solubility oxygen in ultraviolet ray, the water and chlorine ion concentration or the like.
Thereby this area presses for a kind of prolonged preservation at room temperature of exploitation and to environment tolerance, and can be more easily mixes with liquid state such as water, food or feed or pulverous medium or be dissolved in wherein novel probiotic composition.
Summary of the invention
Technical problem
Therefore, main purpose of the present invention provides a kind of probiotic composition that contains food dye, this probiotic composition can be kept the physiologically active of functional microbial wherein long-term effectively, and can be more easily mix with liquid state such as water, food or feed or pulverous medium or be dissolved in the above-mentioned medium, use with convenient.
Another object of the present invention is for a kind of probiotic composition that contains skimming milk is provided, this probiotic composition is the physiologically active of maintenance package functional microbial with which long-term effectively, and can be more easily mix with liquid state such as water, food or feed or pulverous homogeneous media ground or be dissolved in equably in the above-mentioned medium, to make things convenient for its use.
Technical scheme
It is a principal object of the present invention to by providing a kind of probiotic composition that contains food dye to realize, this probiotic composition is the physiologically active of maintenance package functional microbial with which long-term effectively, and can more easily mix with liquid state such as water, food or feed or pulverous medium or be dissolved in the above-mentioned medium, to make things convenient for its use.
The used pigment of the present invention is the material that a kind of light wave that reflects specific wavelength presents particular color, and can not produce deleterious effect to the physiological function of animals such as people or ox.
The used pigment of the present invention comprises the pigment of stipulating in the Pharmacopoeia Coreana of foodstuff additive article and KFDA (korean foods drug administration), though and other does not list the foodstuff additive article in and Pharmacopoeia Coreana is stipulated, known edible pigment.
Various pigments are used as the protection thing of ultraviolet radiation and make an addition in the probiotic composition, so that reduce the death that microorganism is taken place as much as possible when probiotic composition dissolves in water, food or feed.
The pigment that joins in the probiotic composition also is used as solubilizing agent simultaneously or helps mixture, and it can make probiotic composition more easily mix with liquid state such as water, food or feed or pulverous medium or be dissolved in the above-mentioned medium.
Pigment added before the lyophilize of living microorganism foodstuff additive.The mixing process of pigment and mentioned microorganism foodstuff additive can not cause disadvantageous effect to the activity that is in the living microorganism between lyophilize or preservation period.
The provide protection of pigment of the present invention sees Table 1.Harmless food dye has been adopted in this test, and has shown the provide protection of edible flavochrome, edible marennin and haematochrome successively.
The different pigments of table 1 are to the provide protection of ultraviolet radiation
| Sample time | Lactic fermentation activity (%) | ||||
| Non-uv-radiation test group | The uv-radiation test group | ||||
| Negative control group | Positive controls | Add the consumption haematochrome of 1% (massfraction) | Add the consumption marennin of 1% (massfraction) | Add the consumption yellow pigment of 1% (massfraction) | |
| 0min | 100 | 100 | 100 | 100 | 100 |
| 30min | 100 | 0 | 31 | 29 | 44 |
| 1h | 100 | 0 | 5 | 13 | 36 |
| 3h | 84 | 0 | 0.6 | 7 | 18 |
| 5h | 66 | 0 | 0.5 | 2.5 | 15 |
| 10h | 53 | 0 | 0.5 | 1.3 | 8 |
Another object of the present invention realizes by a kind of probiotic composition that contains skimming milk is provided, this probiotic composition is the physiologically active of maintenance package functional microbial with which long-term effectively, and can be more easily mixes with liquid state such as water, food or feed or pulverous homogeneous media ground or be dissolved in equably in the above-mentioned medium, with convenient use.
Skimming milk of the present invention is in a liquid state or the meal behind degreaser drying.
Various water such as domestic use of water, underground water, surface water or seawater can be used to dissolve probiotic composition, but these water and the water through purifying are distinguishing in some aspects.That is to say, contained concentration in the above-mentioned water such as chlorion, factors such as solubility oxygen all can influence the activity of living microorganism foodstuff additive.
In order to reduce the influence of above-mentioned various factors as much as possible, often adopt as stablizers such as S-WAT, L-halfcystines.
Yet if probiotic composition is dissolved in the more domestic use of water of chloride ion-containing, this moment, stablizer can't be contained the death of living microorganism food ingredient effectively.
By table 2 as seen, skimming milk more effectively reduces in the domestic use of water chlorion to the influence of living microorganism foodstuff additive than S-WAT, L-halfcystine.
Skimming milk mixed before the lyophilize of living microorganism foodstuff additive.Even if the probiotic composition that has skimming milk is dissolved in the water of chloride ion-containing, it also can keep microbic activity.
The pigment that joins in the probiotic composition also is used as solubilizing agent simultaneously or helps mixture, and it can make probiotic composition more easily mix with liquid state such as water, food or feed or pulverous medium or be dissolved in the above-mentioned medium.
The influence of table 2 S-WAT, L-halfcystine and skimming milk
| Sample time | Lactic fermentation activity (%) | ||||
| PBS damping fluid (pH7.5) | Service water | Service water | |||
| S-WAT | The L-halfcystine | Skimming milk | |||
| 0.1pp m | 1pp m | 10pp m | 0ppm | 0pp m | 0.1% (mass percent) | |||
| 0h | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| 1h | 82 | 97 | 88 | 85 | 88 | 84 | 95 | 96 |
| 3h | 86 | 97 | 72 | 63 | 71 | 58 | 75 | 75 |
| 5h | 61 | 68 | 68 | 53 | 65 | 66 | 68 | 70 |
| 10h | 71 | 43 | 34 | 45 | 41 | 55 | 50 | 68 |
| 24h | 37 | 9 | 0 | 5 | 4 | 6 | 10 | 36 |
What the present invention was feasible will be further elaborated in ensuing embodiment with optimized technical scheme.The present invention is not limited in these embodiment, allows those skilled in the art that it is carried out various modifications and change.
The effect of invention
The dry probiotic composition that comprises food dye or skimming milk among the present invention can easily mix with liquid state such as water, food or feed or pulverous medium or be dissolved in the above-mentioned medium.And can reduce as much as possible such as of the influence of environmental factorss such as ultraviolet ray, dilution water to the microorganism active in the living microorganism foodstuff additive.
Thereby the operator can add living microorganism food ingredient of the present invention relieved, easily when this probiotic bacterium and ox feed are mixed.Probiotic composition of the present invention can play and the present identical effect of living microorganism food ingredient, and this probiotic composition is used for the welcome that actual use will be subjected to market.
Embodiment
Embodiment 1. is with the difference of dissolving water kind, the active change of lactic fermentation, and skimming milk is to the active provide protection of viable bacteria
Respectively with the PBS damping fluid of 0.9% isotonic solution (NaCl be dissolved in the distilled water promptly) or pH7.5 with 0.9% and with service water (service water), seawater or underground water etc. as solvent, relatively with the different solvents dissolving back active difference of lactic fermentation (table 3).The skimming milk that add to surpass 0.05% (mass ratio) in domestic use of water is tested as solvent, and the result shows that the adding of skimming milk can keep lactic fermentation activity (table 4) effectively.
The active variation of lactic fermentation in the various aqueous solution of table 3
| Sample time | Lactic fermentation activity (%) | ||||||
| PBS damping fluid (pH7.5) | Distilled water+0.9% NaCl | 10ppm NaClO+ 0.9％Na Cl | 100ppm NaClO+ 0.9％Na Cl | Underground water | Seawater | Service water | |
| 0h | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| 1h | 82 | 98 | 100 | 0 | 103 | 53 | 97 |
| 3h | 86 | 88 | 93 | 0 | 82 | 28 | 97 |
| 5h | 61 | 70 | 74 | 0 | 87 | 28 | 68 |
| 10h | 71 | 55 | 57 | 0 | 51 | 33 | 43 |
| 24h | 37 | 42 | 29 | 0 | 46 | 26 | 9 |
The provide protection that skimming milk rose that adds different concns in the table 4 dissolving water
| Sample time | Lactic fermentation activity (%) | |||||
| PBS damping fluid (pH7.5) | Service water | Service water+0.05% (massfraction) skimming milk | Service water+0.1% (massfraction) skimming milk | Service water+0.15% (massfraction) skimming milk | Service water+0.15% (massfraction) skimming milk | |
| 0h | 100 | 100 | 100 | 100 | 100 | 100 |
| 1h | 82 | 97 | 94 | 96 | 96 | 100 |
| 3h | 86 | 97 | 83 | 75 | 100 | 83 |
| 5h | 61 | 68 | 77 | 70 | 90 | 88 |
| 10h | 71 | 43 | 38 | 68 | 80 | 88 |
| 24h | 37 | 9 | 18 | 36 | 60 | 57 |
Embodiment 2. food dyes are to the provide protection of uv-radiation
With containing 0.9% (massfraction) NaCl, concentration is that the sodium phosphate buffer (pH7.5) of 50mM dilutes 3000 times with lactic acid bacteria culture.After adding massfraction respectively and be 0.001%, 0.005%, 0.01% and 0.04% edible marennin, under the intensive ultraviolet condition, detect the survival quantity of thalline every 15min, the result shows that the survival rate of milk-acid bacteria increases according to adding shown in the table 5 above after the food dye of 0.005% (massfraction).
Table 5 is under uviolizing, and the food dye of different concns is to the provide protection of milk-acid bacteria
| Sample time | Lactic acid bacterium number (1 * 10 5cfu/g) | ||||
| Control group | Edible marennin concentration (massfraction 0.001%) | Edible marennin concentration (massfraction 0.005%) | Edible marennin concentration (massfraction 0.01%) | Edible marennin concentration (massfraction 0.04%) | |
| 0min | 3400 | 2600 | 3000 | 2300 | 2800 |
| 5min | 1300 | 1100 | 1300 | 1300 | 1800 |
| 10min | 330 | 360 | 520 | 480 | 800 |
| 15min | 79 | 54 | 110 | 140 | 220 |
| 20min | 8.6 | 14 | 31 | 36 | 110 |
| 25min | 2.9 | 1.9 | 8.6 | 12 | 27 |
| 30min | 0.88 | 0.23 | 1 | 2.4 | 6.6 |
| 35min | 0.16 | 0.09 | 0.32 | 0.59 | 2.6 |
| 40min | 0.026 | 0.02 | 0.08 | 0.14 | 1 |
| 45min | 0 | 0 | 0.02 | 0.04 | 0.18 |
| 50min | 0 | 0 | 0 | 0 | 0.04 |
Test shows do not having under the situation of uviolizing, and food dye is to the not influence (table 6) of survival rate of milk-acid bacteria
Table 6 is not having under the situation of uviolizing, the food dye role of different concns
| Time | Lactic acid bacterium number (1 * 10 5cfu/g) | |||
| Control group | Edible marennin concentration (massfraction 0.001%) | Edible marennin concentration (massfraction 0.005%) | Edible marennin concentration (massfraction 0.01%) | |
| 0h | 18 | 23 | 23 | 24 |
| 1h | 18 | 24 | 24 | 24 |
| 3h | 16 | 24 | 23 | 25 |
| 5h | 17.3 | 23 | 25 | 23 |
| 10h | 17 | 22 | 24 | 24 |
| 24h | 7 | 9 | 8.8 | 9 |
| Survival rate during 24h | 39％ | 39％ | 38％ | 38％ |
The discontinuous nursing simultaneous test that embodiment 3. carries out at feedlot
Every bottle of edible marennin that contains 0.5% (massfraction), the skimming milk of 15% (massfraction) and the milk-acid bacteria of 1 * 1010cfu are diluted with domestic use of water.With per 10000 chicken dosage feedings of 1 bottle every day, feeding time is then: after chicken continues feed 5-9 days, 16-21 days and 26-28 days, feeding is 14 days altogether.
, after 30 days chicken is weighed at lasting feeding.Will through the chicken of above-mentioned feeding with compare with the chicken of existing probiotic composition feeding 30 days (feeding every day), find that their mean body weight is identical with survival rate, sees Table 7.This result shows confession animal edible of the present invention, and the milk-acid bacteria probiotic composition that contains food dye and skimming milk is safely and effectively.
Table 7 in the result who carries out discontinuous feeding study with raising
| Classification | Continue the chicken quantity of feed | The chicken quantity of weighing | Survival rate (%) | Mean body weight (kg) | Remarks |
| Test group | 13,400 | 13,290 | 99.2 | 1.295 | Feeding 14 days |
| Control group | 21,600 | 21,450 | 99.3 | 1.248 | Feeding 30 days |
The continuous feeding study that embodiment 4. carries out at feedlot
Every bottle of edible marennin that contains 0.5% (massfraction), the skimming milk of 15% (massfraction) and the milk-acid bacteria of 1 * 1010cfu are with service water (sevice water) dilution.Then, with the dosage feeding chickens of 1 bottle of per 10000 chicken.Continue to feed after 36 days chicken is weighed.With they mean body weight, survival rate and feed uptake rate with compare with the chicken of existing probiotic composition feeding.The results are shown in Table 8.
The survival rate of test group and control group chicken differs and is about 3%, and the feed uptake rate of test group is higher by about 8% than control group.This result shows food dye and the skimming milk of containing of the present invention, is safely and effectively for the milk-acid bacteria probiotic composition of animal edible.
Table 8 is in the result of the continuous feeding study of feedlot
| Classification | Continue the chicken quantity of feed | The chicken quantity of weighing | Survival rate (%) | Mean body weight when weighing (kg) | The feed total amount of taking in | The feed uptake rate |
| Test group | 10,000 | 8,500 | 85 | 1.35 | 20,250 | 1.76 |
| Control group | 21,000 | 18,500 | 88 | 1.37 | 48,300 | 1.90 |
The foregoing description that specification sheets is described is practicable optimal technical scheme for the purpose of the present invention.But the present invention is not limited in the foregoing description, it will be appreciated by those skilled in the art that the technical scheme of the process equivalents described in dependent claims all drops within protection scope of the present invention.
Claims (5)
1, a kind of probiotic composition is characterized in that containing food dye.
2, probiotic composition as claimed in claim 1 is characterized in that described pigment content is 0.005%-5%, and above-mentioned per-cent is massfraction.
3, a kind of probiotic composition is characterized in that containing skimming milk.
4, probiotic composition as claimed in claim 3 is characterized in that described skim milk content is 0.05%-50%, and above-mentioned per-cent is massfraction.
5, a kind of probiotic composition is characterized in that containing food dye and skimming milk.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020040030164A KR20040052720A (en) | 2004-04-29 | 2004-04-29 | Composition of lactobacillus probiotics containing edible pigment and skim milk. |
| KR1020040030164 | 2004-04-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1950497A true CN1950497A (en) | 2007-04-18 |
Family
ID=35241669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2005800138218A Pending CN1950497A (en) | 2004-04-29 | 2005-04-29 | Probiotic composition |
Country Status (3)
| Country | Link |
|---|---|
| KR (1) | KR20040052720A (en) |
| CN (1) | CN1950497A (en) |
| WO (1) | WO2005105980A1 (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5976580A (en) * | 1995-06-07 | 1999-11-02 | Novus International, Inc. | Nutrient formulation and process for enhancing the health, livability, cumulative weight gain or feed efficiency in poultry and other animals |
| JP3046303B1 (en) * | 1999-06-24 | 2000-05-29 | 明治乳業株式会社 | Helicobacter pylori eradication food and drink |
| KR100398665B1 (en) * | 2000-08-09 | 2003-09-19 | 비에이치피에이치 컴퍼니 리미티드 | Lactic acid bacterial preparation |
-
2004
- 2004-04-29 KR KR1020040030164A patent/KR20040052720A/en active Search and Examination
-
2005
- 2005-04-29 CN CNA2005800138218A patent/CN1950497A/en active Pending
- 2005-04-29 WO PCT/KR2005/001254 patent/WO2005105980A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005105980A1 (en) | 2005-11-10 |
| KR20040052720A (en) | 2004-06-23 |
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