CN1947800A - Hemostatic particles and preparation method thereof - Google Patents
Hemostatic particles and preparation method thereof Download PDFInfo
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- CN1947800A CN1947800A CN 200610053680 CN200610053680A CN1947800A CN 1947800 A CN1947800 A CN 1947800A CN 200610053680 CN200610053680 CN 200610053680 CN 200610053680 A CN200610053680 A CN 200610053680A CN 1947800 A CN1947800 A CN 1947800A
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- 230000002439 hemostatic effect Effects 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 239000002245 particle Substances 0.000 title claims abstract description 7
- 229920002472 Starch Polymers 0.000 claims abstract description 30
- 239000008107 starch Substances 0.000 claims abstract description 30
- 235000019698 starch Nutrition 0.000 claims abstract description 22
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229920001592 potato starch Polymers 0.000 claims abstract description 13
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 claims abstract description 9
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 150000004676 glycans Chemical class 0.000 claims abstract description 7
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 7
- 239000005017 polysaccharide Substances 0.000 claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000005662 Paraffin oil Substances 0.000 claims abstract description 5
- 238000004132 cross linking Methods 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 36
- 238000003756 stirring Methods 0.000 claims description 23
- 239000008187 granular material Substances 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 21
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000011259 mixed solution Substances 0.000 claims description 8
- 239000012188 paraffin wax Substances 0.000 claims description 8
- 239000003995 emulsifying agent Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 239000000284 extract Substances 0.000 claims description 4
- 229940057995 liquid paraffin Drugs 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 4
- 239000004615 ingredient Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
- -1 liquid paraffin Substances 0.000 claims description 2
- 238000012856 packing Methods 0.000 claims description 2
- 238000003828 vacuum filtration Methods 0.000 claims description 2
- 208000032843 Hemorrhage Diseases 0.000 abstract description 14
- 210000004369 blood Anatomy 0.000 abstract description 10
- 239000008280 blood Substances 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 6
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 239000012798 spherical particle Substances 0.000 abstract 1
- 238000005507 spraying Methods 0.000 abstract 1
- 230000023597 hemostasis Effects 0.000 description 7
- 239000000463 material Substances 0.000 description 6
- 239000011521 glass Substances 0.000 description 5
- 230000000025 haemostatic effect Effects 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 108090000190 Thrombin Proteins 0.000 description 3
- 239000004531 microgranule Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 231100000957 no side effect Toxicity 0.000 description 3
- 229960004072 thrombin Drugs 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 description 2
- 108010073385 Fibrin Proteins 0.000 description 2
- 102000009123 Fibrin Human genes 0.000 description 2
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000015271 coagulation Effects 0.000 description 2
- 238000005345 coagulation Methods 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 230000001804 emulsifying effect Effects 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 229950003499 fibrin Drugs 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- FBPFZTCFMRRESA-ZXXMMSQZSA-N D-iditol Chemical compound OC[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-ZXXMMSQZSA-N 0.000 description 1
- 108010080379 Fibrin Tissue Adhesive Proteins 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 101710185622 Pyrrolidone-carboxylate peptidase Proteins 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 238000009455 aseptic packaging Methods 0.000 description 1
- 230000008952 bacterial invasion Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000004627 regenerated cellulose Substances 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Landscapes
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
A hemostatic particle and its preparation method are provided, the medicinal component of the hemostatic particle is a spherical particle with 30-98 micron surface micropores formed by cross-linking microporous polysaccharide extracted from potato starch. The preparation method comprises the following steps: plant starch is gelatinized, emulsified by span80 under the condition of pH 8-10, dispersed by paraffin oil, crosslinked by epichlorohydrin, separated by ethyl acetate, cleaned and dehydrated by absolute ethyl alcohol, dried by an oven, filled in a medicinal spraying device, sterilized by ethylene oxide and applied to clinical use. The hemostatic particles have good biocompatibility and hemostatic effect, are particularly suitable for large-area blood seepage, deep hemorrhage and hemorrhage of parts which are difficult to reach in operation, have easily available raw materials, simple process and low production cost, and can meet the requirements of industrial production.
Description
(1) technical field the present invention relates to a kind of medical hemostatic micro-granules and preparation method thereof.
(2) background technology local hemostasis material is extremely important, and as the injured rescue of the emergency treatment of daily sudden accident, particularly war, hospital all usually will use the local hemostasis material in to patient's wound hemostasis.To the hemorrhage main employing binder compressing of extremity bleeding part, discover recently and use the field operations dressing of the new hemostatic mechanism that is different from direct compressing can reduce severe haemorrhage at present.Before the ideal institute dressing should have that cost is low, good stability, easy to use, characteristics such as haemostatic effect is good, can form simultaneously moistening, heat insulation environment with prevention bacterial invasion and absorption wound exudate and pollutant, and remove and be unlikely to destroy new epithelize and blood crusts from wound easily.Use collagen-based composite, fibrin product, chitosan and zeolite that more general bleeding-stopping dressing has traditional alginate, collagen and occurs recently at present.The Taehocomd that Linz, AUT city HafsLund Nycomed pharmaceuticals develops can absorb blood stanching of wound surface agent, Gelfix hemostatic material and Surgieel regenerated cellulose hemostatic material etc.Its haemostatic effect has all fully been verified in surgical operation and zoopery.Simultaneously, experiment in vitro has also been proved its good anthemorrhagic performance.But its medicinal ingredient mainly is thrombin of beef that extracts etc. in animal body, dangerous.
Surgical hemostasis is one of core of surgical technic, and good hemostatic technique is the key that guarantees that operation is successful.Along with of the raising of various countries' medical circle to hemostatic material anthemorrhagic performance and the requirement of full peace property, seek the good biomaterial of occurring in nature, develop haemostatic effect better, have no side effect, nonirritant, be easy to the bleeding-stopping dressing of machine-shaping, imperative.
(3) summary of the invention task of the present invention is to utilize the agricultural product potato starch to extract micropore polysaccharide to prepare hemostatic micro-granules, this hemostatic micro-granules good biocompatibility, have no side effect, haemostatic effect is good, is applicable to the large tracts of land oozing of blood, the deep is hemorrhage and operation technique is difficult to reach the hemorrhage of position.The present invention also provides the preparation method of this hemostatic micro-granules.
The medicinal ingredient of the hemostatic micro-granules provided by the invention spheroidal particle that 30～98 microns surface band micropore is arranged that to be the micropore polysaccharide that extracts from potato starch form after crosslinked.
The preparation method of hemostatic micro-granules of the present invention:
1) produce starch solution: get the potato starch of constant weight unit, add 10～50 times of distilled water, the starch solution after the stirring is fully adjusted pH to 8.0～10.0 with NaOH solution; Perhaps, drip NaOH solution in the starch solution after the gelatinizing fully, adjust pH to 8.0～10.0 the constant temperature gelatinizing in 50～70 ℃ of temperature water-baths of the starch solution behind the adding distil water;
2) produce paraffin oil-starch mixed solution: get span80 emulsifying agent, liquid paraffin, mix, in 50～70 ℃ of thermostat water baths, stirred 5-30 minute, join the step poly-1 then) starch solution in, stirring and evenly mixing 5-30 minute, the envelope-bulk to weight ratio of paraffin oil and potato starch, ml/g is 25～200: 1; The weight ratio of span80 emulsifying agent and potato starch, g/g is 0.1～2.5: 1.
3) crosslinking Treatment: add the chloropropylene oxide of certain volume unit in above-mentioned paraffin oil-starch mixed solution, stirring reaction 1～10 hour, the ratio of chloropropylene oxide and the volume weight of potato starch, ml/g is 0.05～2.5: 1;
4) preparation hemostatic micro-granules: above-mentioned product standing demix, take off a layer milky white liquid, add ethyl acetate, addition is 4～5 times of lower floor's milky white liquid, stirs; Standing demix takes off a layer milky white liquid again, adds the dehydrated alcohol of 2～3 times of primary liquid, restir, and vacuum filtration to moisture content is drained then, and the pressed powder drying that obtains, screening back packing promptly obtain product.
Advantage of the present invention is:
The micropore polysaccharide that from potato starch, extracts, one of formation has the surface of 30-98 micron to have the spheroidal particle (being called for short the MPH hemostatic micro-granules) of micropore after passing through technical finesse such as crosslinked grade, nontoxic, have no side effect, good biocompatibility, the composition that does not contain any animal sources or humanized, and be aseptic packaging.This granule makes the blood fast dewatering after being directly used in the active hemorrhage position, thereby thrombin, erythrocyte and platelet are concentrated, and promotes the clot of instantaneity to form, can be hemorrhage at the trunk that 1 minute inner control comprises tremulous pulse.
The effect of hemostatic micro-granules possess hydrophilic property molecule filter screen of the present invention.By assembling the solid constituent in the blood,, around granule, form a kind of colloidal mixture, thereby quickened natural coagulation process as plasma proteins such as platelet, erythrocyte, albumin, thrombin and Fibrinogens.Anastalsis took place in several minutes usually.Its degradation process in vivo begins after hemostasis takes place, and multiple factor is depended in its absorption, comprises the dosage of use and the position of use.When being used for soft tissue, hemostatic micro-granules is absorbed in a couple of days fully, without any tissue reaction.
The interaction property of product of the present invention is pure physical property, and this material has very high Absorption to liquid, and the absorption of the moisture in the blood is caused hematoblastic concentrating, and strengthens its coagulation speed and agglutinability; Water sorption equally also reduces the liquid volume of wound site fast, helps accumulative generation.After the imbibition, peripheral vessels is formed certain pressure and promotes hemostasis; The suction back forms waterproof gel shape, shutoff wound.This polysaccharide microgranule can promote solidifying of blood simultaneously, and electron scanning confirms that also fibrin sticks to around the microgranule.The polysaccharide microgranule can be very fast resolved into maltose and glucose by intravital amylase and PYRASE, can not become the cradle of infection, do not cause adhesion yet.
The MPH hemostatic micro-granules can be used for various surgical operations as a kind of auxiliary hemostasis device.When in that pressurization, ligation or other routine operation are invalid maybe can not implement the time, can help to control the hemorrhage of blood capillary, vein and tremulous pulse.
Compare with Fibrin Glue, chemosynthesis glue, have haemostatic effect more efficiently, be particularly useful for the large tracts of land oozing of blood, the hemorrhage and operation technique in deep is difficult to reach the hemorrhage of position.
(4) specific embodiments
Further specify the present invention below by example, but invention is not limited thereto.
Embodiment 1:
1) produce starch solution:
Accurately take by weighing edible Rhizoma Solani tuber osi (Rhizoma Solani tuber osi) starch 4.0g with electronic balance, measure 40～200ml distilled water with graduated cylinder, mix homogeneously in the 250ml beaker;
In above-mentioned starch solution, drip the NaOH solution of 1mol/L, observe in pH value of solution=8.0～10.0 scopes with the pH reagent paper;
2) produce paraffin oil-starch mixed solution:
The above-mentioned starch solution that mixes up pH value, disperse with span80 emulsifying and paraffin oil, promptly accurately get span80 emulsifying agent 0.4～10.0g with electronic balance, measure liquid paraffin 100～1200ml with graduated cylinder, join in the ground flask, stirring rod is installed, the ground flask is placed 50～70 ℃ thermostat water bath, begin to stir, mixing speed is about 500rpm; After stirring 5～30min, add the starch solution of above-mentioned preparation, continue to stir 5～30min;
3) crosslinking Treatment:
Measure chloropropylene oxide 0.2～8.0ml with pipet, add in paraffin oil-starch mixed solution, (the optimization time is 2～4h) to stirring reaction 1～10h;
4) hemostatic micro-granules:
Above-mentioned product is poured in the 1000ml beaker, leaves standstill, about 2h, treat the solution layering after, upper solution is toppled over, the milky white liquid A of lower floor continues to employ;
In lower floor's milky white liquid, add ethyl acetate, separatory, addition approximately is 4～5 times of lower floor's milky white liquid, stirs with Glass rod, pours in the 1000ml separatory funnel and leaves standstill separatory, takes off a layer milky white liquid B;
Add dehydrated alcohol among the milky white liquid B of lower floor behind separatory, addition is 2～3 times of the milky white liquid B of lower floor, stirs with Glass rod, and sand core funnel is connected with vacuum pump, and the startup water pump is poured solution into the sand core funnel sucking filtration, drains to moisture;
The pressed powder that sucking filtration obtains takes out and places culture dish, places 40 ℃ Constant Temp. Oven to dry on culture dish;
Get the pressed powder after the oven dry, the sub-sieve screening with 30.8～98 μ m is product.
Accurately weighing is loaded on medicinal sprayer unit, airtight package, ethane via epoxyethane sterilization back warehouse-in.
Embodiment 2
1) produce gelatinization of starch solution:
Accurately take by weighing edible Rhizoma Solani tuber osi (Rhizoma Solani tuber osi) starch 4.0g with electronic balance, measure the 100ml distilled water with graduated cylinder, mix homogeneously in the 250ml beaker;
The above-mentioned beaker for preparing solution is placed 50 ℃ thermostat water bath, and heating in water bath keeps 5min, and continues slowly to use glass stirring rod agitating solution, is starchiness to solution, takes out beaker and is cooled to room temperature, promptly obtains gelatinizing starch solution completely;
In gelatinization of starch solution, drip the NaOH solution of 1mol/L, observe in pH value of solution=9.0 scopes with the pH reagent paper;
2) produce paraffin oil-starch mixed solution:
The above-mentioned gelatinization of starch solution that mixes up pH value, disperse with span80 emulsifying and paraffin oil, promptly accurately get span80 emulsifying agent 1.6g with electronic balance, measure liquid paraffin 400ml with graduated cylinder, join in three mouthfuls of ground flasks, stirring rod is installed, three mouthfuls of ground flasks are placed 65 ℃ thermostat water bath, begin to stir, mixing speed is 500rpm; After stirring 15min, add the gelatinization of starch solution of above-mentioned preparation, continue to stir 15min;
3) crosslinking Treatment:
Measure chloropropylene oxide 0.8ml with pipet, add in paraffin oil-starch mixed solution, the stirring reaction time is 2h;
4) hemostatic micro-granules:
Above-mentioned product is poured in the 1000ml beaker, leaves standstill 2h, treat the solution layering after, upper solution is toppled over, the milky white liquid A of lower floor continues to employ;
In lower floor's milky white liquid, add ethyl acetate, separatory, addition approximately is 4～5 times of lower floor's milky white liquid, stirs with Glass rod, pours in the 1000ml separatory funnel and leaves standstill separatory, takes off a layer milky white liquid B;
Add dehydrated alcohol among the milky white liquid B of lower floor behind separatory, addition is 2～3 times of the milky white liquid B of lower floor, stirs with Glass rod, and sand core funnel is connected with vacuum pump, and the startup water pump is poured solution into the sand core funnel sucking filtration, drains to moisture;
The pressed powder that sucking filtration obtains takes out and places culture dish, places 40 ℃ Constant Temp. Oven to dry on culture dish;
Get the pressed powder after the oven dry, the sub-sieve screening with 30.8～98 μ m is product.
Accurately weighing is loaded on medicinal sprayer unit, airtight package, ethane via epoxyethane sterilization back warehouse-in.
Claims (3)
1. a hemostatic micro-granules is characterized in that, the medicinal ingredient of the hemostatic micro-granules spheroidal particle that 30～98 microns surface band micropore is arranged that to be the micropore polysaccharide that extracts from potato starch form after crosslinked.
2. the preparation method of hemostatic micro-granules according to claim 1 is characterized in that carrying out according to following processing step:
1) produce starch solution: get the potato starch of constant weight unit, add 10～50 times of distilled water, the starch solution after the stirring is fully adjusted pH to 8.0～10.0 with NaOH solution; Perhaps, drip NaOH solution in the starch solution after the gelatinizing fully, adjust pH to 8.0～10.0 the constant temperature gelatinizing in 50～70 ℃ of temperature water-baths of the starch solution behind the adding distil water;
2) produce paraffin oil-starch mixed solution: get span80 emulsifying agent, liquid paraffin, mix, in 50～70 ℃ of thermostat water baths, stirred 5-30 minute, join the step poly-1 then) starch solution in, stir 5-30 and divide clock time, the envelope-bulk to weight ratio of paraffin oil and potato starch, ml/g is 25～200: 1; The weight ratio of span80 emulsifying agent and potato starch, g/g is 0.1～2.5: 1.
3) crosslinking Treatment: add the chloropropylene oxide of certain volume unit in above-mentioned paraffin oil-starch mixed solution, stirring reaction 1～10 hour, the ratio of chloropropylene oxide and the volume weight of potato starch, ml/g is 0.05～2.5: 1;
4) preparation hemostatic micro-granules: above-mentioned product standing demix, take off a layer milky white liquid, add the ethyl acetate separatory, addition is 4～5 times of lower floor's milky white liquid, stirs; Standing demix takes off a layer milky white liquid again, and the dehydrated alcohol that adds 2～3 times cleans, restir, and vacuum filtration to moisture content is drained then, and the pressed powder drying that obtains, screening back packing promptly obtain product.
3. the preparation method of hemostatic micro-granules according to claim 1 is characterized in that the gelatinization time in step 1) is 1～10 minute.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100536809A CN100453122C (en) | 2006-09-29 | 2006-09-29 | Hemostatic particles and preparation method thereof |
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| CNB2006100536809A CN100453122C (en) | 2006-09-29 | 2006-09-29 | Hemostatic particles and preparation method thereof |
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| CN1947800A true CN1947800A (en) | 2007-04-18 |
| CN100453122C CN100453122C (en) | 2009-01-21 |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102139123A (en) * | 2011-03-25 | 2011-08-03 | 杜宝堂 | Method for preparing intra-operative hemostatic material by cross emulsification of plant starch |
| CN102302796A (en) * | 2011-09-08 | 2012-01-04 | 江苏天麟生物医药科技有限公司 | Absorptive hemostatic biological material and preparation method thereof |
| CN102406956A (en) * | 2011-03-09 | 2012-04-11 | 天津爱勒易医药材料有限公司 | Starch hemostatic microsphere and preparation method thereof |
| CN101361986B (en) * | 2007-08-09 | 2013-06-05 | 纪欣 | Modified starch absorbable or biocompatible hemostasia material and preparation method thereof |
| CN104225662A (en) * | 2014-09-28 | 2014-12-24 | 沈丽青 | Hemostatic product and preparation method thereof |
| CN104274486A (en) * | 2013-09-03 | 2015-01-14 | 江西丽华鑫朗药业科技有限公司 | Composite biodegradable styptic powder prepared by sodium hyaluronate |
| CN104606723A (en) * | 2014-12-12 | 2015-05-13 | 重庆联佰博超医疗器械有限公司 | Absorbable starch bleeding-stopping powder, and preparation method and application thereof |
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| FR2783429B1 (en) * | 1998-09-18 | 2002-04-12 | Imedex Biomateriaux | BICOMPOSITE COLLAGENIC MATERIAL, ITS OBTAINING PROCESS AND ITS THERAPEUTIC APPLICATIONS |
| MXPA04006438A (en) * | 2001-12-31 | 2005-06-08 | Ares Lab Llc | Hemostatic compositions and methods for controlling bleeding. |
| US7019191B2 (en) * | 2003-03-25 | 2006-03-28 | Ethicon, Inc. | Hemostatic wound dressings and methods of making same |
| US20040241212A1 (en) * | 2003-05-30 | 2004-12-02 | Pendharkar Sanyog Manohar | Biodegradable hemostatic wound dressings |
| AU2004253463B2 (en) * | 2003-06-16 | 2010-12-09 | Loma Linda University Medical Center | Deployable multifunctional hemostatic agent |
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Assignee: Hangzhou Singclean Medical Products Co., Ltd. Assignor: Shen Jing Contract record no.: 2010330001603 Denomination of invention: Hemostatic micro-granules and its prepn. method Granted publication date: 20090121 License type: Exclusive License Open date: 20070418 Record date: 20100809 |
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Effective date of registration: 20160830 Address after: 311215, No. 10, East (125) Street, Xiasha economic and Technological Development Zone, Hangzhou, Zhejiang Patentee after: Hangzhou Singclean Medical Products Co., Ltd. Address before: 311402 Gaoqiao Industrial Park (Hefeng), Fuyang, Zhejiang Patentee before: Shen Jing |