CN107802881A - A kind of plant polysaccharide with anastalsis and preparation method thereof - Google Patents
A kind of plant polysaccharide with anastalsis and preparation method thereof Download PDFInfo
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- CN107802881A CN107802881A CN201611116747.9A CN201611116747A CN107802881A CN 107802881 A CN107802881 A CN 107802881A CN 201611116747 A CN201611116747 A CN 201611116747A CN 107802881 A CN107802881 A CN 107802881A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/08—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/716—Glucans
- A61K31/718—Starch or degraded starch, e.g. amylose, amylopectin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
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Abstract
The invention belongs to pharmaceutical technology field, and the invention provides a kind of plant polysaccharide with anastalsis and preparation method thereof, the polysaccharide is prepared by following raw materials：Plant amylum, sorbester p18 or sorbester p17, paraffin oil, epoxychloropropane, chloroacetic acid, maleic acid；The polysaccharide that the present invention obtains, there is the advantages of anthemorrhagic speed is fast, haemostatic effect is good, safe.
Description
Technical field
The invention belongs to medical science or medical instruments field, and in particular to a kind of plant polysaccharide with anastalsis
And preparation method thereof.
Background technology
Traumatic bleeding is one of most common condition of the injury of battlefield and all kinds of scenes of the accident, and bleeding out of control is to cause wounded scene dead
The first cause died.Even if the serious wounded of most bleedings are sent to hospital and rescued, massive blood loss can still cause higher before institute
The death rate and serious complication, thus at the scene with the bleeding wounded are effectively stopped blooding before institute it is significant.For a long time,
Based on traditional pre hospital care method is stopped blooding with tourniquet, tourniquet is the buoyant apparatus that can control bleeding, but for human body very
Multiple location, such as chest, abdomen, head, neck, tourniquet can not use or invalid.(such as cotton yarn and taut in addition, traditional hemostatic material
Band) it is very undesirable for the irregular surface of a wound, the haemostatic effect of the common wound in the scene such as particularly deep, narrow, arteriorrhexis.Therefore,
Exploitation uses for scene and pre hospital care, and quick, safe and effective new hemostatic material turns into medical science and biomaterial section
An important topic in field.
As medical science, biotechnology and materialogy constantly come out in the development of 21 century, various new hemostatic materials.By
Gradually converted from nonabsorable to absorbable direction, converted from animal sources to plant source direction, gradually turned from protide to carbohydrate
Change.Absorbable hemostatic material product category is various, and commercialized product mainly has microfibrillar collagen, medical hemostatic gelatin, cyano group at present
Acrylic compounds tissue glue, protide hemostatic material and polysaccharide hemostatic material etc., its application in clinical and daily life
It is extremely wide.The hemostatic materials such as Fibrin Glue, medical hemostatic gelatin, microfibrillar collagen and collagenous fiber network have animal sources
Property, can induce patient occurs allergic reaction, higher potential application risk be present.Alpha-cyanoacrylate class loading glue class hemostatic material
The noxious materials such as cyanogen and formaldehyde are discharged in degradation process, inflammatory reaction or wound healing can be caused to postpone, existed larger
Potential safety hazard, limit its extensive use in war, wound and daily life.Polysaccharide Absorbable hemostatic material is nature
In a large amount of existing natural macromolecular materials, there is abundance, cheap, good biocompatibility, easily dropped in human body
The many advantages such as solution absorbs, adverse reaction is few, turn into the study hotspot of current hemostatic material.
The method that patent CN201110073208 prepares hemostatic material in art using plant amylum through cross emulsification, is disclosed
A kind of method for preparing hemostatic material in art through cross emulsification using plant amylum, this method are the warps using plant amylum as raw material
Gelatinization, enzymolysis, emulsification and cross linked, solvent extraction, washing, spray drying screening, sterilization packaging can obtain hemostatic material.Using this hair
The hemostatic material of bright preparation has excellent biological barrier performance and histocompatbility, can be done directly on the blood surface of a wound, for
The use of the organ and tissue of complex contour is particularly convenient.Selected materials are plant amylum, have it is non-toxic, nonirritant, come
Source is abundant, cheap, simple production process is feasible, and rate of water absorption is fast, and bleeding stopping period is short, and using effect is good, has wide
Potential applicability in clinical practice.A kind of preparation methods of hemostatic material of patent CN200810225502, a kind of preparation method of hemostatic material,
This method includes with crosslinking agent reacting in organic solution reactant, by obtained reaction product progress separation of solid and liquid, and does
The dry solid product isolated, the reactant contain starch and water, wherein, the organic solution be containing phosphate and/or
The solution of phosphate ester salt and organic solvent.The rate of water absorption for the hemostatic material being prepared using the method for the present invention is higher,
It can play a part of stopping blooding rapidly during practical application.Patent CN200810009706 denaturated starch absorbable hemostatic materials and
Its preparation method, a kind of denaturated starch absorbable hemostatic material and preparation method thereof, described hemostatic material are denatured for etherificate
Starch, or etherificate, one kind in crosslinking composite modified starch or two or more composition, molecular weight 15,000~
2,000,000, grain diameter is 10~1000 μm, and water absorbent rate is 1~100 times.Advantage is：Biocompatible hemostatic material is straight
Connect for there is the blood surface of a wound, stop blooding immediately, water absorbent rate, absorption speed significantly improve, and have bigger viscosity and viscosity, hemostasis is together
When play a part of it is plugging damaged tissue and blood vessel.It is easy to be swelled or be dissolved in water, normal saline flushing is used after hemostasis, removes
The hemostatic material of hemostasis is had neither part nor lot in, reduces residual in vivo, beneficial to wound healing, is avoided caused by tearing gauze, bandage
Pain.Hemostatic material is stablized, is not easily decomposed, long shelf-life, be easy to storage, high pressure resistant, low pressure, high temperature resistant, it is low temperature resistant, be not easy to change
Become physicochemical property.Patent CN200610053680, one kind are related to a kind of hemostatic micro-granules and preparation method thereof, hemostatic micro-granules it is medicinal
Composition be the micropore polysaccharide extracted from farina it is crosslinked after formed have 30~98 microns of surface with micropore
Spheric granules.Its preparation method：By plant amylum through gelatinization, under the conditions of pH8-10 through span80 emulsification and paraffin oil dispersed,
Epichlorohydrin cross-linked, ethyl acetate liquid separation, washes of absolute alcohol dehydration, oven drying forms, loaded in medicinal sprayer unit, through ring
After oxidative ethane sterilizing, applied to Clinical practice.Hemostatic micro-granules of the present invention, good biocompatibility, haemostatic effect, it is particularly suitable for use in big
Area oozing of blood, Hemorrhage in Deep and operation technique are difficult to the bleeding for reaching position, and raw material of the invention is easy to get, and technique is simple, production
Cost is low, can meet demand of industrial production.Starch gelatinization is carried out cross-linking reaction or enzymolysis post-crosslinking by above-mentioned patent, is obtained
For spheric granules of the surface with micropore as hemostatic material, the spherex hemostasis speed obtained using cross-linking reaction is slower.
The content of the invention
For these reasons, applicant is by multiple creative experiment, obtain a kind of security is higher, hemostasis speed more
Much faster glycan, polysaccharide of the invention are by plant amylum, sorbester p18 or sorbester p17, paraffin oil, epoxychloropropane, a chloroethene
The raw materials such as acid, maleic acid are prepared, the polysaccharide that preparation method of the present invention obtains, have anthemorrhagic speed is fast, haemostatic effect is good,
The advantages of safe.
What the present invention was achieved through the following technical solutions.
A kind of polysaccharide, the polysaccharide are prepared by following raw materials：Plant amylum, sorbester p18 or sorbester p17, paraffin oil,
Epoxychloropropane, chloroacetic acid, maleic acid.
Preferably raw material is：Wherein plant amylum 2-6 parts by weight, sorbester p18 or sorbester p17 1-3 parts by weight, paraffin oil
140-220 parts by weight, epoxychloropropane 0.4-0.6 parts by weight, chloroacetic acid 0.4-0.6 parts by weight, maleic acid 0.1-0.2 weight
Part.
Further preferred raw material is：The parts by weight of plant amylum 4, sorbester p18 or the parts by weight of sorbester p17 2, the weight of paraffin oil 180
Measure part, the parts by weight of epoxychloropropane 0.5, the parts by weight of chloroacetic acid 0.5, the parts by weight of maleic acid 0.15.
The preparation method of plant polysaccharide described above is：
Step (1) takes plant amylum, adds water 50-70 parts by weight, adds pH adjusting agent A and adjusts pH value 9-11,20-30 points of stirring
Clock, in 65-75 DEG C of stirred in water bath to gelatinization；
Step (2) takes sorbester p18 or sorbester p17 to add in paraffin oil, and 50 DEG C -70 DEG C are stirred until homogeneous, after adding gelatinization
Plant amylum, stir, add epoxychloropropane, in 50 DEG C of -70 DEG C of stirring 2-4 hours；
Step (3) add pH adjusting agent B 5-10 parts by weight, stir, place 20-40 minutes, add chloroacetic acid and
Maleic acid, stir 1.5-2.5 hours；Ethanol is added, precipitation is complete, sediment drying, obtains polysaccharide.
PH adjusting agent A wherein in step (1) is sodium hydroxide.
PH adjusting agent B wherein in step (3) is sodium citrate.
Wherein in step (3), after adding ethanol, 5-10 DEG C is cooled to, precipitation is complete, and sediment adjusts pH=with citric acid
5.0-6.0 ethanol washs 2-4 times, drying, obtains polysaccharide.
A kind of application of polysaccharide described above in treatment haemostatic medicament or hemostasis medical or medicine equipment is prepared.Institute
The plant polysaccharide with anastalsis is stated, can be used as medicine, can be used for the use of hemostasis medical technology, can also use
Used in medicine equipment.A kind of application of polysaccharide in treatment haemostatic medicament or medicine equipment is prepared.
Plant amylum described above includes but is not limited to：Cornstarch, farina, green starch, starch from sweet potato,
Pueraria starch, konjak starch etc..
Embodiment
Test 1 external coagulating effectiveness experiment
Trial drug：The present invention prepares embodiment and obtains polysaccharide；
The hemostatic micro-granules of CN200610053680.9 embodiments 1.
Test method：SD rats weigh weight, the amount of anesthetic according to needed for calculating weight, the hydration chlorine of intraperitoneal injection 10%
Aldehyde；After SD rat anesthesias, thoracic cavity is opened, heart blood is gathered with syringe, injects in anticoagulant tube, it is standby；The sheet that will be weighed up
The hemostatic micro-granules 2mg of inventive embodiments polysaccharide 2mg and CN200610053680.9 embodiment 1 is added in test tube, then is added thereto
Enter 1.8ml anticoagulated whole blood solution, finally add 0.18ml CaCl thereto2Solution, start timing；Blood change is observed, directly
There is obvious curdled appearance to blood, stop timing, record the clotting time, each sample surveys 8 Duplicate Samples.
Result of the test is shown in Table 1.
The external haemostatic effect comparing result of table 1
Note：The * P compared with CN200610053680.9 embodiments 1<0.05.
Conclusion (of pressure testing)：Above-mentioned experiment shows that hemoglutination of the invention will be significantly better than comparative example, absolutely prove the present invention
Polysaccharide has more preferable anastalsis
Test the docking haemostatic effect experiment of 2 mouse
Trial drug：The present invention prepares embodiment and obtains polysaccharide；
The hemostatic micro-granules of CN200610053680.9 embodiments 1.
Test method：Polysaccharide sample made from 0.05g embodiments is weighed respectively and 0.1g CN200610053680.9 are real
The hemostatic micro-granules of example 1 are applied, are respectively placed in displaying bottle, sterilizing is standby；The C57BL/6 mouse of same batch are taken, are weighed, measure tail length；
After 1/3rd that mouse tail length is cut off with operation, docking wound is inserted in displaying bottle immediately, while timing.Blood flow is calculated to stop
Mouse amount of bleeding when only.
Result of the test is shown in Table 2.
The mouse of table 2 docking haemostatic effect result of the test
Note：The * P compared with CN200610053680.9 embodiments 1<0.05.
Conclusion (of pressure testing)：Above-mentioned experiment shows that polysaccharide of the present invention has more outstanding hemostasis than comparative example hemostasis granules
Effect.
Test degraded and safety research in 3 bodies
Trial drug：The present invention prepares embodiment 3 and obtains polysaccharide；
The hemostatic micro-granules of CN200610053680.9 embodiments 1.
Degraded in vivo：Test method：Reference：(1) GBT16886.6-2003 BiologicalEvaluationofMedicalDevices, the 16th part：
The Drug Pharmacokinetics research and design of catabolite and leachable thing；(2) GBT16886.6-1997 BiologicalEvaluationofMedicalDevices,
6th part：Local reaction is tested after implantation.Cleaning grade healthy SD rat, weight 180-200g.SD rats are taken to be grouped at random
(3 groups of the embodiment of the present invention, the hemostatic micro-granules group of CN200610053680.9 embodiments 1, every group 12), 3 groups of the embodiment of the present invention,
The hemostatic micro-granules group of CN200610053680.9 embodiments 1, chloraldurate (400mg/kg) anesthetized rat is injected intraperitoneally, back is shaved
Hair, twice 2cm × 0.3cm otch is done in backbone both sides, and otch, will to otch outside separation hypodermis 2cm at a distance of 1cm
The polysaccharide of 0.1g embodiments 3 or hemostasis granules are imbedded in the backbone hypodermis of left side, and right side is control sides, is sewed up the incision, just
Often raising SD rats.1d, 3d, 7d, 14d after implantation, 3 rats are respectively put to death, cut backbone both sides original notch respectively
Position, iodine solution is added dropwise in former heeling-in micropore vacuum polysaccharide hemostatic microsphere position, observation styptic powder is degraded situation in tissue.With
Bush rat muscular tissue after the implantation of-eosin staining procedures optical microphotograph Microscopic observation changes.
Result of the test：It is shown in Table 3.
Degradation results compare in the body of table 3
Conclusion (of pressure testing)：Above-mentioned experiment shows that present invention hemostasis polysaccharide is in 3d, completion of having degraded, and
CN200610053680.9 hemostatic micro-granules 14d could degrade completion, further illustrate that polysaccharide of the present invention has preferably safety
Property.
Safety evaluation：Test method：SD rats are taken to be randomly divided into the embodiment of the present invention 3 group 10 and control group 5, this
3 groups of inventive embodiments, control group are ditto anaesthetized, back shaving, and doing 2 otch in backbone bilateral (is about 5cm, is deep to flesh
Layer 0.3cm), ibid to separation hypodermis 2cm on the outside of otch, whole wound is covered in muscle wound sprinkling 0.1g polysaccharides,
Sew up the incision, control group does not give hemostasis polysaccharide, normally raises 14d.In postoperative 14d, collection administration group blood carries out following
Detection, liver function：Total bilirubin (TB), glutamic-oxalacetic transaminease (AST), glutamic-pyruvic transaminase (ALT), creatinine (Cr), urea nitrogen
(BUN)。
Result of the test is shown in Table 4.
The rat blood biochemistry detection result of table 4
Conclusion (of pressure testing)：Above-mentioned experiment shows that the biochemical indicator of test group of the present invention and control group does not have significant difference, enters
One step explanation present invention hemostasis polysaccharide has good security.
Prepare embodiment
Embodiment 1
Polysaccharide with anastalsis, it is prepared by following raw materials：Farina 200g, sorbester p17 100g, stone
Wax oil 14500g, epoxychloropropane 40g, chloroacetic acid 40g, maleic acid 10g.
Polysaccharide preparation method is：
Step (1) takes plant amylum, adds water 5000g, adds pH adjusting agent A sodium hydroxides and adjusts pH value 9, stirs 20 minutes,
In 65 DEG C of stirred in water bath to gelatinization；
Step (2) takes sorbester p17 to add in paraffin oil, and 50 DEG C are stirred until homogeneous, the plant amylum added after gelatinization, stirring
Uniformly, epoxychloropropane is added, is stirred 4 hours at 50 DEG C；
Step (3) adds pH adjusting agent B sodium citrate 500g, stirs, and places 20 minutes, adds chloroacetic acid and horse
Carry out sour, stirring 1.5 hours；Ethanol is added, is cooled to 5 DEG C, precipitation is complete, and sediment adjusts pH=5.0 ethanol to wash with citric acid
Wash 2 times, dry, obtain polysaccharide.
Embodiment 2
Polysaccharide with anastalsis, it is prepared by following raw materials：Green starch 600g, sorbester p18 300g, paraffin
Oily 22000g, epoxychloropropane 60g, chloroacetic acid 60g, maleic acid 20g.
Polysaccharide preparation method is：
Step (1) takes plant amylum, adds water 7000g, adds pH adjusting agent A sodium hydroxides and adjusts pH value 11, stirs 30 minutes,
In 75 DEG C of stirred in water bath to gelatinization；
Step (2) takes sorbester p18 to add in paraffin oil, and 70 DEG C are stirred until homogeneous, the plant amylum added after gelatinization, stirring
Uniformly, epoxychloropropane is added, is stirred 4 hours at 70 DEG C；
Step (3) adds pH adjusting agent B sodium citrate 1000g, stirs, and places 40 minutes, adds chloroacetic acid, horse
Carry out sour, stirring 2.5 hours；Ethanol is added, is cooled to 5-10 DEG C, precipitation is complete, and sediment adjusts pH=6.0 ethanol with citric acid
Washing 4 times, drying, obtains polysaccharide.
Embodiment 3
Polysaccharide with anastalsis, it is prepared by following raw materials：Farina 400g, sorbester p18 200g, stone
Wax oil 18000g, epoxychloropropane 50g, chloroacetic acid 50g, maleic acid 15g.
Polysaccharide preparation method is：
Step (1) takes plant amylum, adds water 6000g, adds pH adjusting agent A sodium hydroxides and adjusts pH value 10, stirs 25 minutes,
In 70 DEG C of stirred in water bath to gelatinization；
Step (2) takes sorbester p18 to add in paraffin oil, and 60 DEG C are stirred until homogeneous, the plant amylum added after gelatinization, stirring
Uniformly, epoxychloropropane is added, is stirred 3 hours at 60 DEG C；
Step (3) adds pH adjusting agent B sodium citrate 750g, stirs, and places 30 minutes, adds chloroacetic acid, horse
Carry out sour, stirring 2 hours；Ethanol is added, is cooled to 7 DEG C, precipitation is complete, and sediment adjusts pH=5.5 ethanol to wash 3 with citric acid
It is secondary, drying, obtain polysaccharide.
Embodiment 4
Polysaccharide with anastalsis, it is prepared by following raw materials：Pueraria starch 250g, sorbester p17 150g, paraffin
Oily 16000g, epoxychloropropane 45g, chloroacetic acid 45g, maleic acid 12g.
Polysaccharide preparation method is：
Step (1) takes plant amylum, adds water 5500g, adds pH adjusting agent A sodium hydroxides and adjusts pH value 9.5, stirs 25 points
Clock, in 70 DEG C of stirred in water bath to gelatinization；
Step (2) takes sorbester p17 to add in paraffin oil, and 65 DEG C are stirred until homogeneous, the plant amylum added after gelatinization, stirring
Uniformly, epoxychloropropane is added, is stirred 2.5 hours at 55 DEG C；
Step (3) adds pH adjusting agent B sodium citrate 600g, stirs, and places 25 minutes, adds chloroacetic acid, horse
Carry out sour, stirring 2 hours；Ethanol is added, is cooled to 6 DEG C, precipitation is complete, and sediment adjusts pH=5.5 ethanol to wash 3 with citric acid
It is secondary, drying, obtain polysaccharide.
Embodiment 5
Polysaccharide with anastalsis, it is prepared by following raw materials：Starch from sweet potato 200g, sorbester p18 100g, paraffin
Oily 14000g, epoxychloropropane 40g, chloroacetic acid 40g, maleic acid 10g.
Polysaccharide preparation method is：
Step (1) takes plant amylum, adds water 650g, adds pH adjusting agent A sodium hydroxides and adjusts pH value 10.5, stirs 25 points
Clock, in 75 DEG C of stirred in water bath to gelatinization；
Step (2) takes sorbester p18 to add in paraffin oil, and 55 DEG C are stirred until homogeneous, the plant amylum added after gelatinization, stirring
Uniformly, epoxychloropropane is added, is stirred 4 hours at 65 DEG C；
Step (3) adds pH adjusting agent B sodium citrate 750g, stirs, and places 35 minutes, adds chloroacetic acid, horse
Carry out sour, stirring 2 hours；Ethanol is added, is cooled to 8 DEG C, precipitation is complete, and sediment adjusts pH=5.5 ethanol to wash 4 with citric acid
It is secondary, drying, obtain polysaccharide.
Embodiment 6
Polysaccharide with anastalsis, it is prepared by following raw materials：Cornstarch 400g, sorbester p17 200g, paraffin
Oily 18000g, epoxychloropropane 50g, chloroacetic acid 50g, maleic acid 18g.
Polysaccharide preparation method is：
Step (1) takes plant amylum, adds water 6000g, adds pH adjusting agent A sodium hydroxides and adjusts pH value 10, stirs 25 minutes,
In 70 DEG C of stirred in water bath to gelatinization；
Step (2) takes sorbester p17 to add in paraffin oil, and 60 DEG C are stirred until homogeneous, the plant amylum added after gelatinization, stirring
Uniformly, epoxychloropropane is added, is stirred 3 hours at 60 DEG C；
Step (3) adds pH adjusting agent B sodium citrate 750g, stirs, and places 30 minutes, adds chloroacetic acid, horse
Carry out sour, stirring 2 hours；Ethanol is added, is cooled to 7 DEG C, precipitation is complete, and sediment adjusts pH=5.5 ethanol to wash 3 with citric acid
It is secondary, drying, obtain polysaccharide.
Below by way of the description of embodiment, the invention will be further described, but this is not the limit to the present invention
System, those skilled in the art according to the present invention basic thought, various modifications may be made or improve, but without departing from this
The basic thought of invention, within the scope of the present invention.
Claims (9)
- A kind of 1. polysaccharide, it is characterised in that：The polysaccharide is prepared by following raw materials：Plant amylum, sorbester p18 or sapn 80th, paraffin oil, epoxychloropropane, chloroacetic acid, maleic acid.
- 2. a kind of polysaccharide according to claim 1, wherein plant amylum 2-6 parts by weight, sorbester p18 or sorbester p17 1-3 Parts by weight, paraffin oil 140-220 parts by weight, epoxychloropropane 0.4-0.6 parts by weight, chloroacetic acid 0.4-0.6 parts by weight, Malaysia Sour 0.1-0.2 parts by weight.
- 3. a kind of polysaccharide according to claim 1, the wherein parts by weight of plant amylum 4, sorbester p18 or the weight of sorbester p17 2 Part, the parts by weight of paraffin oil 180, the parts by weight of epoxychloropropane 0.5, the parts by weight of chloroacetic acid 0.5, the parts by weight of maleic acid 0.15.
- 4. according to a kind of polysaccharide described in claim any one of 1-3, it is characterised in that：The preparation method of plant polysaccharide For：Step (1) takes plant amylum, adds water 50-70 parts by weight, adds pH adjusting agent A and adjusts pH value 9-11, stirs 20-30 minutes, In 65-75 DEG C of stirred in water bath to gelatinization；Step (2) takes sorbester p18 or sorbester p17 to add in paraffin oil, and 50 DEG C -70 DEG C are stirred until homogeneous, the plant added after gelatinization Starch, stir, add epoxychloropropane, in 50 DEG C of -70 DEG C of stirring 2-4 hours；Step (3) adds pH adjusting agent B 5-10 parts by weight, stirs, and places 20-40 minutes, adds chloroacetic acid and Malaysia Acid, stir 1.5-2.5 hours；Ethanol is added, precipitation is complete, sediment drying, obtains polysaccharide.
- 5. a kind of polysaccharide according to claim 4, wherein pH adjusting agent A are sodium hydroxide.
- 6. a kind of polysaccharide according to claim 4, wherein pH adjusting agent B are sodium citrate.
- 7. in a kind of polysaccharide according to claim 4, wherein step (3), after adding ethanol, being cooled to 5-10 DEG C, sink Form sediment complete, sediment adjusts pH=5.0-6.0 ethanol to wash 2-4 times with citric acid, dries, obtains polysaccharide.
- A kind of 8. the answering in treatment haemostatic medicament or medicine equipment is prepared of polysaccharide according to claim any one of 1-4 With.
- 9. according to a kind of polysaccharide described in claim any one of 1-7, wherein plant amylum includes cornstarch, potato is formed sediment Powder, green starch, starch from sweet potato, pueraria starch, konjak starch.
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| CN112843324A (en) * | 2021-01-13 | 2021-05-28 | 山东省药学科学院 | Preparation method of rapidly degradable hemostatic powder |
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| CN1947800A (en) * | 2006-09-29 | 2007-04-18 | 沈晶 | Hemostatic particles and preparation method thereof |
| CN102526794A (en) * | 2012-01-19 | 2012-07-04 | 华东理工大学 | Calcium-complex starch-based microporous haemostatic material, and preparation method and application thereof |
| CN106075556A (en) * | 2016-06-02 | 2016-11-09 | 四川奎星医用高分子制品有限责任公司 | Containing the medical recombination chitosan gel promoting wound healing medicine |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112843324A (en) * | 2021-01-13 | 2021-05-28 | 山东省药学科学院 | Preparation method of rapidly degradable hemostatic powder |
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