Summary of the invention
The purpose of this invention is to provide little bupleurum preparation a kind of suitable large-scale industrial production, that have good stability; comprise soft capsule, tablet, capsule and granule, it is the preparation of being made by the medical material of following weight proportioning: Radix Bupleuri 210~270g, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) 60~120g, Radix Scutellariae 60~120g, Radix Codonopsis 60~120h, Radix Glycyrrhizae 60~120g, Rhizoma Zingiberis Recens 60~120g, Fructus Jujubae 60~120g.
Another object of the present invention has provided the preparation method of little bupleurum preparation.Its preparation method is as follows:
(1) extraction of thick paste:
1. extraction process one: measure Radix Bupleuri, Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, the clean medical material of the Fructus Jujubae five tastes by prescription and add 6~12 times of water, decoct 1~2 time, and each 1~3 hour, filter, filtrate is concentrated in right amount; Measure Rhizoma Zingiberis Recens, the Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) of handling well by prescription, add the ethanol of 4~8 times of amounts 50~90%, decoct 1~2 time, each 0.5~1.5 hour, reclaim ethanol, gained extracting solution and above-mentioned concentrated solution merge, and get thick paste.
2. extraction process two: Radix Bupleuri, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, Fructus Jujubae seven flavor medicine, Radix Codonopsis and Radix Glycyrrhizae are ground into fine powder for each 16~32 parts, standby, remaining Radix Codonopsis, Radix Glycyrrhizae and Radix Bupleuri, Radix Scutellariae, Fructus Jujubae add water and decoct 2 times for 6~12 times, each 1~2 hour, filter, filtrate is concentrated in right amount; The Rhizoma Pinelliae, Rhizoma Zingiberis Recens are made solvent by percolation with 50~90% ethanol, flood after 12~36 hours, with per minute 0.5~5ml speed percolation, reclaim ethanol, and gained extracting solution and above-mentioned concentrated solution merge, and add Radix Codonopsis and Radix Glycyrrhizae fine powder, get thick paste.
3. extraction process three: Radix Bupleuri, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, Fructus Jujubae seven flavor medicine, Radix Bupleuri, Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Fructus Jujubae add water and decoct 2 times for 6~12 times, and each 1~2 hour, filter, filtrate is concentrated in right amount; The Rhizoma Pinelliae, Rhizoma Zingiberis Recens are made solvent by percolation with 50~90% ethanol, flood after 12~36 hours, with per minute 0.5~5ml speed percolation, reclaim ethanol, and gained extracting solution and above-mentioned concentrated solution merge, and get thick paste.
(2) preparation of little bupleurum preparation (comprising soft capsule, tablet, capsule and granule):
1. soft capsule: get the water that gelatin under agitation adds 0.9~1.5 times of amount, treat that gelatin dissolves the back fully and adds 0.2~0.7 times of glycerol, 0~0.1 times of PEG400, stir, the insulation of degassing back is left standstill and is promptly got softgel shell; Get the thick paste of recipe quantity, spray drying, powder becomes fine powder, adds 0.8~2 times substrate, and stirring promptly gets medicinal liquid; The soft capsule shell of above-mentioned preparation and medicinal liquid are dropped into soft capsule produce in the filling machine and be pressed into soft capsule, after dry 24 hours, dry packing.
2. tablet: get the thick paste of recipe quantity, add 5~15% micropowder silica gels, 5% calcium hydrogen phosphate, 10~20% dextrin, mixing, drying, powder becomes fine powder, makes granule, and drying adds 0.5~1% micropowder silica gel, tabletting, packing.
3. capsule: get the thick paste of recipe quantity, add 5~15% micropowder silica gel, mixing, drying, powder becomes fine powder, packing.
4. granule: get the thick paste of recipe quantity, add 4~8 times sucrose, mixing is made granule, drying, packing.
Another object of the present invention has provided the application of little bupleurum preparation aspect treatment hepatitis, and is mainly as follows:
(1) to carbon tetrachloride (CCl
4) due to the influence of rats'liver damage: 83 of SD rats are selected in experiment for use, and body weight 227.3 ± 14.2 grams divide 7 groups of (blank, CCl
4Respectively one group of modeling, bifendate, little bupleurum particles, 3 groups of little Radix Bupleuri capsules), except that the normal control group is 11, all the other every group is 12, and every day, gastric infusion was 1 time, continuous 14 days, behind the 4th day medicine 1 hour, except that normal group, every Mus back subcutaneous injection 15%CCl
4Solution 2ml/kg injected commensurability CCl later on every 3 days
4Once, totally 4 times.In the 3rd, 4 injection CCl
4Back hungry 16 hours is respectively got 10 animals for every group and is got blood 2ml by the tail vein, and separation of serum also detects ALT, AST and bilirubin value.Test to put to death animal on the 15th day and cut open the belly immediately and get liver and do histological examination.The results are shown in Table 1.Inject 3 times CCl
4Serum ALT, the AST of back modeling group rat obviously increase, and bilirubin level still belongs to normal range, and this moment, little Radix Bupleuri capsule of large, medium and small dosage and little bupleurum particles all had the effect of obvious reduction Serum ALT, AST vigor; Injection the 4th CCl
4After (total amount 112g/kg) 16 hours, except that the heavy dose of group of little Radix Bupleuri capsule and bifendate group can obviously reduce the ALT value, other each groups are compared zero difference with the modeling group, and AST value that medication is respectively organized is compared no difference of science of statistics with the modeling group.CCl is shown in histological examination
4Modeling group liver obviously damages, hepatocyte cloudy swelling, balloon sample pathological changes, and pathological changes is attached most importance to around the central veins of hepatic lobules; The hepatic lesions of each administration group dosage group in little Radix Bupleuri capsule made moderate progress, all the other each groups were not seen notable difference.
The little Radix Bupleuri capsule/granule of table 1 is to CCl
4Due to the influence (x ± s) of rats'liver damage
Group | Dosage (g/kg) | n | Injection CCl
43 times
| Injection CCl
44 times
|
ALT(IU) | AST(IU) | Bilirubin (mg%) | ALT(IU) | AST(IU) | Bilirubin (mg%) |
Blank CCl
4The little bupleurum particles of the little Radix Bupleuri capsule of the little Radix Bupleuri capsule of the little Radix Bupleuri capsule of modeling bifendate
| 0.06 15 10 5 10 | 11 12 12 12 12 12 12 | 115±18 237±66
△△ 159±21
△△** 144±27
△△** 171±52
△△** 192±48
△△* 195±42
△△** | 244±20 344±32
△△ 278±60
** 282±46
△** 283±46
△** 320±42
△△ 284±32
△△** | 0.10±0.06 0.19±0.01
△ 0.13±0.12 0.10±0.08
* 0.13±0.10 0.12±0.06 0.15±0.11
| 66±45 170±30
△△ 78±59
** 120±36
△△** 144±36
△△ 156±42
△△ 135±63
△ | 240±22 312±64
△△ 282±42
△ 272±24
△△ 279±43
△ 278±32
△△ 272±20
△△ | 0.17±0.03 0.26±0.14
△ 0.20±0.11 0.17±0.08 0.18±0108 0.20±0.11 0.20±0.06
|
Compare with the blank group,
△P<0.05,
△ △P<0.01; With CCl
4The modeling group is compared,
*P<0.05,
*P<0.01
(2) to the influence of rats'liver infringement due to the galactosamine: 97 male and female half and half of SD rat are selected in experiment for use, and body weight 175.1 ± 16.4 grams divide 7 groups of (blank, CCl
4Respectively one group of modeling, bifendate, little bupleurum particles, 3 groups of little Radix Bupleuri capsules), except that the normal control group is 13, all the other every group is 14, and dosage is the same.Every day, gastric infusion was 1 time, continuous 6 days.After administration in the 4th day 1 hour, except that the normal control group, every Mus injection GalN 600mg/kg, each group was got 6~8 rat extracting blood separation of serum respectively in then 24,48,96 hours, measure ALT, AST and bilirubin, and behind injection GalN 48,96 hours, dead 4~6 rats in each component other places are cut open the belly and get liver and do histological examination.Table 2 show the injection GalN after 24 hours, modeling group and each administration group Serum ALT, AST vigor all obviously raise than normal group, but each the group between do not have significant difference; Behind the injection GalN 48 hours, modeling group ALT, AST continued rising, and the big or middle dosage group of little Radix Bupleuri capsule and this two enzyme activity of little bupleurum particles group are compared obvious decline with the modeling group; Behind the injection GalN 96 hours, the ALT of each administration group, AST recovered normally, and the modeling group only ALT recover normally.The bilirubin level of each treated animal belongs to normal range.Histological examination shows that modeling group lobule periphery hepatocyte cloudy swelling companion cavity becomes; 96 hours pathological changes was light than 48 hours behind the injection GalN; The rat liver pathological changes of little Radix Bupleuri capsule of various dose and little bupleurum particles is similar to the modeling group, but the cloudy swelling degree is light slightly.
The little Radix Bupleuri capsule/granule of table 2 is to the influence of rats'liver infringement due to the GalN (x ± s)
Group | Dosage (g/kg) | Modeling 24h | Modeling 48h | Modeling 96h |
ALT(IU) | AST(IU) | Bilirubin (mg%) | ALT(IU) | AST(IU) | Bilirubin (mg%) | ALT(IU) | AST(IU) | Bilirubin (mg%) |
Blank CCl
4The little bupleurum particles of the little Radix Bupleuri capsule of the little Radix Bupleuri capsule of the little Radix Bupleuri capsule of modeling bifendate
| 0.06 15 10 5 10 | 90±18 178±12
△△ 177±33
△△ 177±12
△△ 176±12
△△ 174±11
△△ 178±10
△△ | 200±28 242±12
△△ 242±30
△△ 244±8
△△ 244±8
△△ 252±30
△△ 244±16
△△ | 0.13±0.09 0.19±0.04 0.19±0.08 0.17±0.08 0.19±0.09 0.19±0.08 0.18±0.13 | 102±30 344±42
△△ 288±60
△△* 274±96
△△* 282±86
△△* 312±84
△△ 278±90
△△* | 200±24 312±42
△△ 288±42
△△ 260±28
△△** 260±40
△△* 252±26
△△** 264±30
△△* | 0.09±0.07 0.14±0.08 0.19±0.08 0.12±0.08 0.15±0.16 0.10±0.09 0.15±0.15 | 102±13 101±15 90±12 120±36 114±18 112±26 105±22 | 196±8 254±40
△△ 208±19
** 212±32
* 220±18
* 200±39
** 202±13
** | 0.09±0.07 0.15±0.09 0.10±0.10 0.13±0.10 0.12±0.06 0.13±0.09 0.14±0.08 |
Compare with the normal control group,
△P<0.05,
△ △P<0.01
The specific embodiment
The following examples will be further explained the present invention, but the present invention is not limited only to these embodiment, the scope that these embodiment do not limit the present invention in any way.
Embodiment 1 is measured Radix Bupleuri, Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, the clean medical material of the Fructus Jujubae five tastes by prescription and is added 6 times of water, decocts 1 hour, filters, and filtrate is concentrated in right amount; Measure Rhizoma Zingiberis Recens, the Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) of handling well by prescription, add the ethanol of 4 times of amounts 50%, decocted 0.5 hour, reclaim ethanol, gained extracting solution and above-mentioned concentrated solution merge, and get thick paste.
Embodiment 2 is measured Radix Bupleuri, Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, the clean medical material of the Fructus Jujubae five tastes by prescription and is added 8 times and 6 times of water, decocts 2 times, and each 2 hours, filter, filtrate is concentrated in right amount; Press recipe quantity Rhizoma Zingiberis Recens, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), add the ethanol of 6 times and 4 times amounts 70%, decoct 2 times, each 1 hour, reclaim ethanol, gained extracting solution and above-mentioned concentrated solution merge, and get thick paste.
Embodiment 3 is measured Radix Bupleuri, Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, the clean medical material of the Fructus Jujubae five tastes by prescription and is added 10 times and 8 times of water, decocts 2 times, and each 2 hours, filter, filtrate is concentrated in right amount; Measure Rhizoma Zingiberis Recens, the Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) of handling well by prescription, add the ethanol of 8 times and 6 times amounts 70%, decoct 2 times, each 1.5 hours, reclaim ethanol, gained extracting solution and above-mentioned concentrated solution merge, and get thick paste.
Embodiment 4 is measured Radix Bupleuri, Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, the clean medical material of the Fructus Jujubae five tastes by prescription and is added 12 times and 10 times of water, decocts 2 times, and each 3 hours, filter, filtrate is concentrated in right amount; Measure Rhizoma Zingiberis Recens, the Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens) of handling well by prescription, add the ethanol of 8 times and 6 times amounts 90%, decoct 2 times, each 1.5 hours, reclaim ethanol, gained extracting solution and above-mentioned concentrated solution merge, and get thick paste.
Embodiment 5 Radix Bupleuri, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, Fructus Jujubae seven flavor medicine, Radix Codonopsis and Radix Glycyrrhizae are ground into fine powder for each 16 parts, and be standby, remaining Radix Codonopsis, Radix Glycyrrhizae and Radix Bupleuri, Radix Scutellariae, Fructus Jujubae add 8 times in water and 6 times and decoct 2 times, each 1 hour, filter, filtrate is concentrated in right amount; The Rhizoma Pinelliae, Rhizoma Zingiberis Recens are made solvent by percolation with 50% ethanol, flood after 12 hours, with per minute 0.5ml speed percolation, reclaim ethanol, and gained extracting solution and above-mentioned concentrated solution merge, and add Radix Codonopsis and Radix Glycyrrhizae fine powder, get thick paste.
Embodiment 6 Radix Bupleuri, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, Fructus Jujubae seven flavor medicine, Radix Codonopsis and Radix Glycyrrhizae are ground into fine powder for each 24 parts, and be standby, remaining Radix Codonopsis, Radix Glycyrrhizae and Radix Bupleuri, Radix Scutellariae, Fructus Jujubae add 10 times in water and 8 times and decoct 2 times, each 2 hours, filter, filtrate is concentrated in right amount; The Rhizoma Pinelliae, Rhizoma Zingiberis Recens are made solvent by percolation with 70% ethanol, flood after 24 hours, with per minute 1.5ml speed percolation, reclaim ethanol, and gained extracting solution and above-mentioned concentrated solution merge, and add Radix Codonopsis and Radix Glycyrrhizae fine powder, get thick paste.
Embodiment 7 Radix Bupleuri, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, Fructus Jujubae seven flavor medicine, Radix Codonopsis and Radix Glycyrrhizae are ground into fine powder for each 32 parts, and be standby, remaining Radix Codonopsis, Radix Glycyrrhizae and Radix Bupleuri, Radix Scutellariae, Fructus Jujubae add 12 times in water and 10 times and decoct 2 times, each 1 hour, filter, filtrate is concentrated in right amount; The Rhizoma Pinelliae, Rhizoma Zingiberis Recens are made solvent by percolation with 90% ethanol, flood after 36 hours, with per minute 3ml speed percolation, reclaim ethanol, and gained extracting solution and above-mentioned concentrated solution merge, and add Radix Codonopsis and Radix Glycyrrhizae fine powder, get thick paste.
Embodiment 8 Radix Bupleuri, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, Fructus Jujubae seven flavor medicine, Radix Codonopsis and Radix Glycyrrhizae are ground into fine powder for each 16 parts, and be standby, remaining Radix Codonopsis, Radix Glycyrrhizae and Radix Bupleuri, Radix Scutellariae, Fructus Jujubae add 12 times in water and 10 times and decoct 2 times, each 2 hours, filter, filtrate is concentrated in right amount; The Rhizoma Pinelliae, Rhizoma Zingiberis Recens are made solvent by percolation with 70% ethanol, flood after 36 hours, with per minute 5ml speed percolation, reclaim ethanol, and gained extracting solution and above-mentioned concentrated solution merge, and add Radix Codonopsis and Radix Glycyrrhizae fine powder, get thick paste.
Embodiment 9 Radix Bupleuri, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, Fructus Jujubae seven flavor medicine, Radix Codonopsis and Radix Glycyrrhizae are ground into fine powder for each 24 parts, and be standby, remaining Radix Codonopsis, Radix Glycyrrhizae and Radix Bupleuri, Radix Scutellariae, Fructus Jujubae add 10 times in water and 8 times and decoct 2 times, each 2 hours, filter, filtrate is concentrated in right amount; The Rhizoma Pinelliae, Rhizoma Zingiberis Recens are made solvent by percolation with 70% ethanol, flood after 24 hours, with per minute 1.5ml speed percolation, reclaim ethanol, and gained extracting solution and above-mentioned concentrated solution merge, and add Radix Codonopsis and Radix Glycyrrhizae fine powder, get thick paste.
Embodiment 10 Radix Bupleuri, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, Fructus Jujubae seven flavor medicine, Radix Bupleuri, Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Fructus Jujubae add 8 times in water and 6 times and doubly decoct 2 times, and each 1 hour, filter, filtrate is concentrated in right amount; The Rhizoma Pinelliae, Rhizoma Zingiberis Recens are made solvent by percolation with 50% ethanol, flood after 12 hours, with per minute 0.5ml speed percolation, reclaim ethanol, and gained extracting solution and above-mentioned concentrated solution merge, and get thick paste.
Embodiment 11 Radix Bupleuri, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, Fructus Jujubae seven flavor medicine, Radix Bupleuri, Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Fructus Jujubae add 10 times in water and 8 times and decoct 2 times, and each 2 hours, filter, filtrate is concentrated in right amount; The Rhizoma Pinelliae, Rhizoma Zingiberis Recens are made solvent by percolation with 70% ethanol, flood after 24 hours, with per minute 1.5ml speed percolation, reclaim ethanol, and gained extracting solution and above-mentioned concentrated solution merge, and get thick paste.
Embodiment 12 Radix Bupleuri, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, Fructus Jujubae seven flavor medicine, Radix Bupleuri, Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Fructus Jujubae add 12 times in water and 10 times and decoct 2 times, and each 1 hour, filter, filtrate is concentrated in right amount; The Rhizoma Pinelliae, Rhizoma Zingiberis Recens are made solvent by percolation with 90% ethanol, flood after 36 hours, with per minute 3ml speed percolation, reclaim ethanol, and gained extracting solution and above-mentioned concentrated solution merge, and get thick paste.
Embodiment 13 Radix Bupleuri, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, Fructus Jujubae seven flavor medicine, Radix Bupleuri, Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Fructus Jujubae add 8 times in water and 6 times and decoct 2 times, and each 2 hours, filter, filtrate is concentrated in right amount; The Rhizoma Pinelliae, Rhizoma Zingiberis Recens are made solvent by percolation with 70% ethanol, flood after 24 hours, with per minute 3ml speed percolation, reclaim ethanol, and gained extracting solution and above-mentioned concentrated solution merge, and get thick paste.
Embodiment 14 Radix Bupleuri, Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, Fructus Jujubae seven flavor medicine, Radix Bupleuri, Radix Scutellariae, Radix Codonopsis, Radix Glycyrrhizae, Fructus Jujubae add 12 times in water and 10 times and decoct 2 times, and each 2 hours, filter, filtrate is concentrated in right amount; The Rhizoma Pinelliae, Rhizoma Zingiberis Recens are made solvent by percolation with 70% ethanol, flood after 24 hours, with per minute 5ml speed percolation, reclaim ethanol, and gained extracting solution and above-mentioned concentrated solution merge, and get thick paste.
Embodiment 15 gets the water that gelatin under agitation adds 0.9 times of amount, treats that gelatin dissolves the back fully and adds 0.2 times of glycerol, stirs, and the insulation of degassing back is left standstill and promptly got softgel shell; Get the thick paste of recipe quantity, spray drying, powder becomes fine powder, adds 0.8 times substrate, stirs; The softgel shell of above-mentioned preparation and medicinal liquid are dropped into soft capsule produce in the filling machine and be pressed into soft capsule, after dry 24 hours, dry and promptly get soft capsule of ' Xiao Chai Hu.
Embodiment 16 gets the water that gelatin under agitation adds 1.1 times of amounts, treats that gelatin dissolves the back fully and adds 0.4 times of glycerol, stirs, and the insulation of degassing back is left standstill and promptly got softgel shell; Get the thick paste of recipe quantity, spray drying, powder becomes fine powder, adds 1.2 times substrate, stirs; The softgel shell of above-mentioned preparation and medicinal liquid are dropped into soft capsule produce in the filling machine and be pressed into soft capsule, after dry 24 hours, dry and promptly get soft capsule of ' Xiao Chai Hu.
Embodiment 17 gets the water that gelatin under agitation adds 1.3 times of amounts, treats that gelatin dissolves the back fully and adds 0.6 times of glycerol, and 00.5 times of PEG400 stirs, and the insulation of degassing back is left standstill and promptly got softgel shell; Get the thick paste of recipe quantity, spray drying, powder becomes fine powder, adds 1.6 times substrate, stirs; The softgel shell of above-mentioned preparation and medicinal liquid are dropped into soft capsule produce in the filling machine and be pressed into soft capsule, after dry 24 hours, dry and promptly get soft capsule of ' Xiao Chai Hu.
Embodiment 18 gets the water that gelatin under agitation adds 1.5 times of amounts, treats that gelatin dissolves the back fully and adds 0.7 times of glycerol, and 0.1 times of PEG400 stirs, and the insulation of degassing back is left standstill and promptly got softgel shell; Get the thick paste of recipe quantity, spray drying, powder becomes fine powder, adds 2 times substrate, stirs; The softgel shell of above-mentioned preparation and medicinal liquid are dropped into soft capsule produce in the filling machine and be pressed into soft capsule, after dry 24 hours, dry and promptly get soft capsule of ' Xiao Chai Hu.
Embodiment 19 gets the thick paste of recipe quantity, adds 5% micropowder silica gel, 5% calcium hydrogen phosphate, 20% dextrin, mixing, 40 ℃ of vacuum dryings, powder becomes fine powder, with 75% ethanol system soft material, cross 20 mesh sieves and make granule, 60 ℃ of dryings, 20 mesh sieve granulate, the micropowder silica gel of adding 0.5%, tabletting, packing promptly gets little Radix Bupleuri sheet.
Embodiment 20 gets the thick paste of recipe quantity, adds 10% micropowder silica gel, 5% calcium hydrogen phosphate, 15% dextrin, mixing, 50 ℃ of vacuum dryings, powder becomes fine powder, with 70% ethanol system soft material, cross 18 mesh sieves and make granule, 65 ℃ of dryings, 18 mesh sieve granulate, the micropowder silica gel of adding 1%, tabletting, packing promptly gets little Radix Bupleuri sheet.
The preparation of embodiment 21 tablets: the thick paste of getting recipe quantity, the micropowder silica gel of adding 15%, 5% calcium hydrogen phosphate, 10% dextrin, mixing, 60 ℃ of vacuum dryings, with 60% ethanol system soft material, cross 16 mesh sieves and make granule, 70 ℃ of dryings, 16 mesh sieve granulate, the micropowder silica gel of adding 1%, tabletting, packing promptly gets little Radix Bupleuri sheet.
Embodiment 22 gets the thick paste of recipe quantity, adds 5% micropowder silica gel, mixing, and 40 ℃ of vacuum dryings, powder becomes fine powder, fill, packing promptly gets little Radix Bupleuri capsule.
The preparation of embodiment 23 capsules: get the thick paste of recipe quantity, add 10% micropowder silica gel, mixing, 50 ℃ of vacuum dryings, powder becomes fine powder, fill, packing promptly gets little Radix Bupleuri capsule.
The preparation of embodiment 24 capsules: get the thick paste of recipe quantity, add 15% micropowder silica gel, mixing, 60 ℃ of vacuum dryings, powder becomes fine powder, fill, packing promptly gets little Radix Bupleuri capsule.
The preparation of embodiment 25 granules: get the thick paste of recipe quantity, add 4 times sucrose, mixing is made granule, 80 ℃ of dryings, and fill, packing promptly gets little bupleurum particles.
The preparation of embodiment 26 granules: get the thick paste of recipe quantity, add 6 times sucrose, mixing is made granule, 70 ℃ of dryings, and fill, packing promptly gets little bupleurum particles.
The preparation of embodiment 27 granules: get the thick paste of recipe quantity, add 8 times sucrose, mixing is made granule, 60 ℃ of dryings, and fill, packing promptly gets little bupleurum particles.