CN1942405A - Methods of inhibiting microorganism growth using moss and devices for water treatments - Google Patents

Methods of inhibiting microorganism growth using moss and devices for water treatments Download PDF

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CN1942405A
CN1942405A CNA2005800115381A CN200580011538A CN1942405A CN 1942405 A CN1942405 A CN 1942405A CN A2005800115381 A CNA2005800115381 A CN A2005800115381A CN 200580011538 A CN200580011538 A CN 200580011538A CN 1942405 A CN1942405 A CN 1942405A
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mosses
water
sphagnum moss
wart
pappus
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CN1942405B (en
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D·奈顿
V·费格尔
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Embro Corp
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/28—Treatment of water, waste water, or sewage by sorption
    • C02F1/286—Treatment of water, waste water, or sewage by sorption using natural organic sorbents or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/42—Nature of the water, waste water, sewage or sludge to be treated from bathing facilities, e.g. swimming pools

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Abstract

The invention provides a method of inhibiting microorganism growth comprising contacting a substance susceptible to microorganism growth with an amount of a non-decomposed moss effective to inhibit microorganism growth, wherein the moss is selected from the group consisting of sphagnum papillosum, sphagnum cristatum, and mixtures therof. The invention provides a device (10) for use in water comprising a carrier having an interior cavity (32) and one or more openings (38, 35) allowing ingress to and egress from the interior cavity (32); and a moss contained within the interior cavity (32) in which the carrier completely encloses the moss.

Description

Adopt mosses to suppress the method and the water treatment device of microorganism growth
Invention field
The present invention relates to adopt mosses, specifically be method and the water treatment method that the peat mosses suppress microorganism growth.
Background of invention
The known various biological control materials in microorganism growth control field.For example, need in food, suppress fungal growth when storing as cereal, and need be at sheathing foodstuff, as bacteria growing inhibiting in the live chickens meat.Absorb in the product such as bandage or feminine hygiene of liquid at other, need comprise antimicrobial composition so that pathogenic biology can not be bred in a large number.
Water treatment is another field of controlling or prevent bacterial growth with chemistry, biology and radiation treatment.The water treatment system that many types are arranged is as the filtration and the cleaning system in swimming pool and fishpond.Many these system filtration water are to remove suspended substance and to reduce the muddy phenomenon of water.Bacterial growth in the anti-sealing and the pollutent of removing in the water are important industry and family problems.For example, answer the cleaned industry waste liquid to remove toxic compounds be discharged in lake and the river at it before and to remove bacterium.Water receptacle such as swimming pool, hot tub, fishpond etc. must keep clean to prevent that water from becoming muddiness and/or wall of container becomes sticky.Available active mode such as strainer come treating water, and to remove particle and bacterium, also available passive mode is come treating water as biocide being put into container and being floated over water.
Usually use chemical mode to keep the cleaning and the minimizing bacterial growth of water.Can adopt ultraviolet ray, chlorine processing, bromine processing, copper and silver ion treatment and ozonize is handled and/or sterilized water.These are typical biocides, promptly destroy material or the energy of living organism.Certainly, must be careful when using all these methods, because they are may be to the user poisonous or damage arranged.Chemical substance needs handled, to avoid contaminate environment and contact user.
" peat mosses " (" Sphagnum moss ") is a kind of general name, is illustrated in a class floristics that coexists in the sphagnum bog.It should be noted that " peat moss (peat moss) " is often referred to the peat mosses of rotting or being used for compost.Usually collect the peat mosses and be used for various products.The preferred hat (petal) of collecting these mosses, but not stem.The general plant materials (root, branch etc.) of removing bulk can further be processed mosses after the collection, promptly form aqueous slurry with the very thin particle of extracting.Remove the water in the slurries, make the mosses drying.Compressible mosses before packing or transportation.Available various additive changes the absorption characteristic or the mechanical characteristics of mosses.Because the peat mosses are easy to obtain, and relatively inexpensive,, be mainly used in absorption liquid so it has been used for various products.
This area is starved of the product that suppresses microorganism such as bacterium, yeast and algal grown.Need a kind of mode and keep water clarification in swimming pool, whirlpool bath, the fishpond etc. for a long time, clean and need not closure systems.The system that wants most only needs considerably less maintenance and relatively cheap.
Summary of the invention
The invention provides a kind of method that suppresses microorganism growth, described method comprises the material of the microorganism that is easy to grow is contacted with the mosses of rotting of the amount that effectively suppresses microorganism growth that wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
The invention provides a kind of method that suppresses microorganism growth, described method comprises to be put into the mosses of rotting that effectively suppress the amount of microorganism growth load bearing component and the material of described load bearing component with the microorganism that is easy to grow is contacted, and wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
The invention provides the method that a kind of preparation is used to suppress the mosses of microorganism growth, described method comprises: (i) make unputrefied mosses drying; (ii) described mosses are sterilized.The invention provides the method that a kind of preparation is used for the mosses of bacteria growing inhibiting, described method comprises: (i) unputrefied mosses are contacted with acidic solution; (ii) make described mosses drying.The invention provides a kind of kit that comprises sterilized do not rot mosses and load bearing component.
The invention provides a kind of method that suppresses microorganism growth, described method comprises the water of the microorganism that is easy to grow is contacted with the mosses of rotting of the amount that effectively suppresses microorganism growth, with regularly impact water with suitable chemical substance, wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
The invention provides a kind of water treatment method, described method comprise with water with effectively remove in the water that the rotten mosses of cationic amount contact except that hydrogen ion, wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.The present invention also provides a kind of water treatment method, described method comprises to be put into load bearing component and described load bearing component is contacted with water effectively removing in the water except that hydrogen ion the mosses of rotting of cationic amount, and wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
The invention provides the device that uses in water, described device comprises: (i) have the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses.
The invention provides a kind of method that suppresses microorganism growth, described method comprises the water of the device that suppresses microorganism growth in the water being put into the bacterium that is easy to grow, and described device comprises: (i) have the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises the mosses of the amount of microorganism growth in effective inhibition water.
The invention provides the kit that comprises the sterilized device that does not rot mosses and in water, use, described device comprises the load bearing component that has inner chamber and one or more permission inflow and flow out the opening of inner chamber, and wherein said inner chamber is sealed the residence fully and stated mosses.
The invention provides a kind of method that suppresses microorganism growth, described method comprises the water of the device that suppresses microorganism growth in the water being put into the microorganism that is easy to grow, with regularly impact water with suitable chemical substance, described device comprises: (i) have the load bearing component that inner chamber and one or more permission flow into and flow out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises the mosses of the amount of microorganism growth in effective inhibition water.
The invention provides a kind of water treatment method, described method comprises puts into water with a device, and described device comprises: (i) have the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises in effective removal water the described mosses of cationic amount except that hydrogen ion.
Should be understood that above-mentioned general description and following detailed description all are exemplary and indicative, be intended to the present invention of prescription is further explained.
Brief Description Of Drawings
Figure 1A has illustrated the skeleton view that can be used for an embodiment of the invention.Figure 1B has illustrated side-view, and Fig. 1 C has illustrated along the sectional view of the C-C line of Figure 1B.
Fig. 2 A has illustrated the skeleton view that can be used for another embodiment of the invention.Fig. 2 B has shown the side-view of the mosses that are used for Fig. 2 A shown device.
Fig. 3 has illustrated the skeleton view that can be used for another embodiment of the invention.
Preferred implementation describes in detail
We have found to can be used for suppressing the sphagnum moss kind of microorganism growth.Available these mosses controls are as the microorganism growth in the water such as swimming pool, Spa, fishpond.Spa be also referred to as the whirlwind-type bathing pool (rotary bathtub, whirlpool) or hot tub.The mosses of believing particular types are to suppressing and/or preventing that bacterium and other microorganism growth are effective especially.
In the present invention, " fungistat " refers to the material of bacteria growing inhibiting.In universaling dictionary, term " antiseptic-germicide " is often referred to the bacterial growth inhibitor.These two terms and " sterilant " should be distinguished, can kill and wound the material of bacterium during the sterilant abutment.
In the present invention, " water treatment " instigate that water in swimming pool, fishpond, bathtub of rotational-flow type, the hot tub etc. keeps clean, clarification and the pleasant technology of smell.When agitate water, it is less to observe foam.Believe that mosses can suppress bacterium and other microorganism growth, the compound and the material of its also adsorbable reduction clarification of water degree.
In the present invention, the wart sphagnum moss (sphagnum papillosum, S.papillosum) and/or the pappus sphagnum moss (sphagnum.cristatum S.cristatum) can be used for water treatment device.In a preferred embodiment, mosses are installed to or are encapsulated into prevent that mosses are in water surrounding in the dispersive Web materials.Therefore, mosses can be remained in the desired location in pond, hot tub, the bathtub of rotational-flow type etc.Preferred Web materials comprises and contains polymkeric substance such as nylon or polyacrylic material, the about 0.1-1mm of sizing grid.Common preferred polymers is because their cheap and degradation resistants.
Be suitable for of the present inventionly being the wart sphagnum moss that from the marsh in the north, Minn., to collect and can be used as compressed package from Sutton ' s Moss of Dobson, Westland, the pappus sphagnum moss that New Zealand buys.These mosses kinds can be separately or one are used from device of the present invention or system.General and preferably, the cleaning mosses are to remove small-particle such as dirt and big fragment such as root and leaf.Commercially available mosses may be handled through sootiness before the manufacturer packs, to destroy seed.In a preferred embodiment, with mechanism mosses are cut into required size and shape.Then, preferably by the high compressed steam method, be exposed to oxyethane or alternate manner well known by persons skilled in the art is sterilized to mosses.Sterilization process has destroyed the living organism in the mosses, thereby prevents to produce in the environment of any bad problem or alien bacteria introducing use apparatus of the present invention.Then, these mosses promptly can be used for water treatment system or other application.
We find that a kind of convenience, easy, effective and cheap water treatment mode are that a part of wart sphagnum moss or pappus sphagnum moss are put into the floatation device that allows water to center on and flow through mosses.The another kind of mode of using it is to be encapsulated in it in the net and to increase the weight of this net, and mosses are stayed in the water.Any suitable method that keeps these mosses to contact with water all is fit to use.Then this device is placed in swimming pool, rotary bathtub, the hot tub etc., can contacts with water at these local devices.We find, this processing prevent aspect bacterial growth and maintenance water cleaning, clarification and tasteless and the non-foam effective especially.Because with impelling water to compare this processing by the filtering system of mosses is passive system, so effect is more unusual.Will of course be appreciated that available apparatus of the present invention are carried out active and filtered treating water.
When being used for swimming pool, hot tub etc., water treatment device described herein preferably with the material coupling that kills and wounds bacterium.This is because these environment may contain a large amount of bacterium load that different time is introduced.Therefore, standard practices be as required filtered water, be filled with water to waterline and test water as required.Available commercial solution is adjusted pH.Water treatment device of the present invention best and oxygenant such as potassium hydrogen persulfate coupling are called " no chlorine impacts ".Known potassium hydrogen persulfate can improve the efficient of chlorine purified product, but we are effective equally especially when finding to use it and above-mentioned peat mosses device coupling.
Peat mosses of the present invention can be used for any composition, material, device or the method that needs suppress microorganism.Application comprises the inhibition microorganism growth, reduces and/or prevents peculiar smell, water treatment, control mould and fungal growth; And control fermentation.This device and material comprise absorbent products, as in the diaper, feminine hygiene, bandage and wound dressing.In these products, these mosses can be packed between the different film of liquid transport property.Promptly (for example) skim can be that liquid is saturable, and another tunic can be a vapor permeable.These mosses can be mixed in the polymkeric substance and are used as face shield.These mosses can be encapsulated in the film and be used for food preservation (preservation) product as absorbing the wrapping material and the lining of the gentle flavor of liquid.These mosses can be used for the cleaning of water-treated prod with water among maintenance hold-up vessel, fishpond, swimming pool, rotary bathtub, the spa etc., and are used for water filtering device.These mosses can be used for black water and handle.These mosses can be made into the shape of (for example) disk or agglomerate, and be used for absorbing the water of cereal and other food.These mosses also can be used for fermentation control (as liquid or particulate state).These mosses can be used for controlling fungi or the microbial diseases in lawn and the garden.These mosses can be used for (as) mould control product in storage vessel or the tubing system.
The invention provides a kind of method that suppresses microorganism growth, described method comprises the material of the microorganism that is easy to grow is contacted with the mosses of rotting of the amount that effectively suppresses microorganism growth that wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.Described mosses can be compressed, and can be bar shapeds.Described mosses can be sterilized or sterilize with ethylene oxide treatment by the sterilization of high compressed steam method, chemical treatment.Described mosses can be through acidic solution, especially acetic acid solution washing.Described mosses can be washed with salts solution after the acidic solution washing.
The material of microorganism of being easy to grow can be a water.Water can be the water in spa, pool or the fishpond.The material of microorganism of being easy to grow can be a part such as skin, surface wound, inner coelom or the internal injury position of human body.The material of microorganism of being easy to grow can be a cereal.
The invention provides a kind of method that suppresses microorganism growth, described method comprise will effectively suppress the mosses of rotting of amount of microorganism growth be placed in the load bearing component and the material of described load bearing component with the microorganism that is easy to grow contacted, wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.Described load bearing component can be device, polymeric matrix, biological substrate or one or more layers film of implant into body.
The invention provides the method that preparation is used to suppress the mosses of microorganism growth, described method comprises: (i) make unputrefied mosses drying; (ii) described mosses are sterilized.This method also can comprise the described mosses of compression, compresses described mosses and described mosses are cut into inch strips, and by high compressed steam method, chemical treatment or ethylene oxide treatment described mosses is sterilized.Described mosses can be the peat mosses.Described mosses can be selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
The invention provides the method that a kind of preparation is used for the mosses of bacteria growing inhibiting, described method comprises: the mosses of (i) will not rotting contact with acidic solution; (ii) make described mosses drying.This method contacts unputrefied mosses after can being included in step (i) with salts solution.In one embodiment, described acidic solution is an acetic acid solution.Described mosses can be the peat mosses.Described mosses can be selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
The invention provides a kind of kit that comprises the do not rot mosses and the load bearing component of sterilization.Described mosses can be compressed, and can be bar shapeds.
The invention provides a kind of method that suppresses microorganism growth, described method comprises the water of the microorganism that is easy to grow is contacted with the mosses of rotting of the amount that effectively suppresses microorganism growth, with regularly impact water with suitable chemical substance, wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.Described chemical substance can be a potassium hydrogen persulfate.
The invention provides a kind of water treatment method, described method comprise with water with effectively remove in the water that the rotten mosses of cationic amount contact except that hydrogen ion, wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.Described positively charged ion can be calcium or iron ion, can remove all calcium or iron ion in the water basically.Described mosses can be compressed, and can be bar shapeds.Described mosses can be sterilized or sterilize with ethylene oxide treatment by the sterilization of high compressed steam method, chemical treatment.Described mosses can be through acidic solution, especially acetic acid solution washing.These mosses can be washed with salts solution after the acidic solution washing.Water can be the water in spa, pool or the fishpond.
The invention provides a kind of water treatment method, described method comprises and is placed in the load bearing component with described load bearing component and contacts with water effectively removing in the water except that hydrogen ion the mosses of rotting of cationic amount that wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
The invention provides the device that uses in water, this device comprises: (i) have the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses.Described load bearing component can comprise float.Described load bearing component can comprise the column part under float and the float, and described column part has inner chamber and one or more permission flows into and the opening of outflow inner chamber.Described mosses can be packed in the mesh bag.Described load bearing component can comprise one or more counterweights.
Described mosses can be unputrefied mosses.Described mosses can be the peat mosses.Described mosses can be selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.Described mosses can be compressed, and can be bar shapeds.Described mosses can be sterilized or sterilize with ethylene oxide treatment by the sterilization of high compressed steam method, chemical treatment.Described mosses can be through acidic solution, especially acetic acid solution washing.Described mosses can be washed with salts solution after the acidic solution washing.
The invention provides a kind of method that suppresses microorganism growth, described method comprises the water of the device that suppresses microorganism growth in the water being put into the microorganism that is easy to grow, and described device comprises: (i) have the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises the mosses of the amount of microorganism growth in effective inhibition water.Water can be the water in spa, pool or the fishpond.Water can pump via described device.
The invention provides the kit of the device that does not rot mosses and in water, use that comprises sterilization, described device comprises the load bearing component that has inner chamber and one or more permission inflow and flow out the opening of inner chamber, and wherein said inner chamber can be sealed the residence fully and state mosses.Described kit can comprise one or more pH calibration tapes and/or potassium hydrogen persulfate.
The invention provides a kind of method that suppresses microorganism growth, described method comprises the water of the device that suppresses microorganism growth in the water being put into the microorganism that is easy to grow, with regularly impact water with suitable chemical substance, described device comprises: (i) have the load bearing component that inner chamber and one or more permission flow into and flow out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises the mosses of the amount of microorganism growth in effective inhibition water.Described chemical substance can be a potassium hydrogen persulfate.
The invention provides a kind of water treatment method, described method comprises puts into water with a device, and described device comprises: (i) have the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises in effective removal water the described mosses of cationic amount except that hydrogen ion.Described positively charged ion can be calcium or iron ion, can remove all calcium or iron ion in the water basically.Described mosses can be compressed, and can be bar shapeds.Described mosses can be sterilized or sterilize with ethylene oxide treatment by the sterilization of high compressed steam method, chemical treatment.Described mosses can be through acidic solution, especially acetic acid solution washing.These mosses can be washed with salts solution after the acidic solution washing.Water can be the water in spa, pool or the fishpond.
Figure 1A-1C has illustrated appropriate device of the present invention.Figure 1A has shown the device 10 that floats in the water, and Figure 1B and 1C have shown side-view and sectional view respectively.Setting device 10 is with the part of the peat mosses 15 of the compression of accepting to be cut into desired size.Dotted line among Figure 1A and the 1B (phantom) expression mosses.The size that makes things convenient for that is used for the mosses of device 10 is about 6 * 1/4 * 1/4 inch (15.2 * 0.63 * 0.63cm).Heavily about 5 grams of mosses of this size.With mosses 15 nylon wire 16 of packing into, the size of nylon wire should allow to compress mosses and expand.Size of mesh opening should be able to keep little mosses particle and prevent that it from breaking apart and float.
Device 10 comprises shock-resistant, water insoluble and can be made into the plastics of desired shape.Device 10 can be used as " float " from Huntington Beach, and the MP Industries of CA buys.It should be noted that such float uses with the agglomerate or the disk of pond sanitising agent such as chloride sanitising agent usually.Make device 10 be fit to use by adding hand-hole to help water to enter this device with the peat mosses.
Device 10 comprises floating part 20 and flows through part 30.Floating part 20 is cylindrical, and can be any desired size, but its diameter is generally greater than flowing through part 30.The used size of floating part is about 5 inches of diameters (12.7cm).
Flowing through part 30 is right cylinders of two portions elongation, and it has the center 32 of core or hollow.First part 33 is connected in floating part 20, in the first part 33 screw thread is arranged, and second section 35 is attached on this screw thread.Can change the length that flows through part in this way.By the fixing position of second section 35 of adjustable collar 34.Second section 35 also is equipped with detachable cap 37, and its effect is to increase the weight of so that install 10 and float in the water according to mode shown in Figure 1.
Slit 38 and hole 39 allow water to flow through this right cylinder.This slit and hole can be any desired sizes, and can locate as required.The usable length that flows through part is about 7 inches (17.8cm).Cap 37 is dismountable, so that can be with the peat mosses insertion portion 30 of required size.The mosses of compression expand once contact water.The density of expansion mosses should be able to make water flow through it.Device 10 is enough to the water (for example, in rotary bathtub or spa) handled up to about 350 gallons reaching in 30 days.
Fig. 2 A explanation floats over the device 50 in the water.Device 50 comprises barrel portion 60, and it has the center 62 of core or hollow.Slit 64 and hole 66 allow water to enter the center of this hollow.As showing the most significantly among Fig. 2 B, with the mosses 55 that show with dotted line among net 52 encapsulation Fig. 2 A.Mosses contact the water after, are full of the center 62 of hollow.The one end sealing of barrel portion 60, the other end is equipped with detachable cap 57.Cap 57 can increase the weight of, and keeps contacting with water so that install 60 maximum length.
Fig. 3 has shown the device 70 of the wall W that is connected in swimming pool, fishpond, hot tub etc.With net 72 encapsulation mosses 75, this net hangs on the carriage 77.The size of mesh opening of this net is enough to make the particle of mosses or fragment to be stayed in the net.Carriage is suspended on the wall, and this device can remain fixed on this position.Perhaps, device 70 can lie in pond or bathtub bottom.It can remain on the below attached to the there or by counterweight.The placement of also it can being connected with strainer.
Embodiment
Embodiment 1
The northern wart sphagnum moss of collecting from the Minnesota State, preparation is used for the bacteriostatic test.This mosses kind is confirmed through University of Minnesota, reaffirms after the acceptance.
All samples is placed in the plastics bag.All undressed mosses are stored in 4 ℃, up to processing by lab assistant.It is stand-by that all pre-dried outside mosses samples are stored in room temperature.Adopt following equipment:
A) agitator, 1.25L capacity (available from the Osterizer  of Oster)
B) distilled water is (available from Premium Water, Inc.)
C) tissue sieve, 1 glass of capacity (available from the Cellector  of E-C Apparatus Corp.)
D) 1L glass beaker (commercially available Pyrex )
E) aseptic polystyrene petri diss 100 * 15mm (available from Falcon)
F) aseptic polystyrene petri diss 150 * 15mm (available from VWR)
G) autoclaving pot (available from Market Forge)
H) the little spoon of metal experiment (16.5cm)/available from the Adison tissue forceps of VWR)
I) laminar flow hood (available from Baker)
Step:
1) from bag, takes out undressed mosses with hand, pick any visible root, leaf and fragment, put into agitator.Be full of this agitator with mosses then, up to the mark line that approximately reaches 1L.
2) then 1L distilled water is added in the agitator, cover lid manually vibrated 30 seconds, and drainage water is to remove any residual dirt and fragment.This process repeats 2 times again with thorough washing mosses.
3) then, again agitator is filled distilled water, mixed mosses in each 30 seconds, distribute up to mosses homogeneous in entire sample with the pulse mode that is set at 4 and 5.
4) discharge water in the agitator then.Extrude all remainder water in the mosses by hand with the tissue sieve then.Then the mosses of extracting are placed in the clean 1L beaker.Repeating step 1-4 piles the mosses of processing in this 1L beaker.Then with this beaker with liquid setting (liquid setting) with 121 ℃ of 15psi (103.4MPa) autoclaving 20 minutes, it is at room temperature cooled off.
5) after the cooling, mosses are moved on in the laminar flow hood, be placed on carefully in the markd petri diss of weighing in advance with little spoon in aseptic experiment chamber and pliers.Need SC in order to avoid pollute these mosses and pack each ware with uniform way.After the packing, make this ware keep opening wide at least 72 hours, up to the mosses drying.Cover the exsiccant ware and be placed on laminar flow hood stand-by.
Embodiment 2
Available from Sutton ' s Moss, the pappus sphagnum moss of Canada (collecting) at New Zealand, preparation is used for the bacteriostatic test.Confirmed this mosses kind after the acceptance.The processing of this mosses sample is identical with the foregoing description 1.Adopt following equipment:
A) asepsis injector, each self-capacity are 30cc or 60cc (available from Becton Dickenson)
B) asepsis injector filter, 0.45 μ m (micron) and 0.20 μ m (available from the Acrodisc of Pall Gellman)
C) Adison tissue forceps (available from VWR)
D) 50cc polypropylene scale test tube, sterile packed (available from Falcon)
E) cere (Parafilm  is available from American National Can)
F) laminar flow hood (available from Baker Company)
G) micropipet of aseptic scale polypropylene tip is housed, 25mL (available from Becton Dickenson)
H) 10M HCl and 10M NaOH
I) pH meter (available from Beckman Omega 40)
Reagent and pharmaceutical solutions: (according to the liquid that is used to handle)
A) Bacto TMTryptic soy broth, 30g/L (Becton Dickenson)/MEM-α (Gibco)
B) phosphate buffered saline (PBS) (1x), pH 7.1 is (available from Gibco TM)
C) hplc grade water (available from J.T.Baker)
Step
A) all liquid that is used to handle is carried out autoclaving or filtration sterilization, be stored under the suitable temperature up to being used for this scheme.In laminar flow hood, finish all processing steps with aseptic technique.Face with preceding used tissue forceps is carried out autoclaving.
B) weigh after the exsiccant mosses, treatment liq is sucked petri diss, concentration is the dry mosses of 1 milliliter of treatment liq/50mg.If adopt the concentration beyond the 50mg/mL, this method of corresponding change.
C) seal the petri diss that mosses are housed with cere (Parafilm ), in 4 ℃ of refrigerators, placed 1 hour.
D) from refrigerator, take out petri diss, take out mosses and clamp pack into 30cc or 60cc syringe with sterile tissue with little spoon.Again insert the piston of this syringe, liquid is clamp-oned in the aseptic 50mL polypropylene test tube, might expressed liquid up to squeezing out.
E) press juice filters by 0.45 μ m syringe filter, then by 0.2 μ m syringe filter, be stored in 4 ℃ stand-by.
F) with the pH of 1.0M HCl/NaOH adjustment filtered sample,, be stored in 4 ℃ once more, measure up to being used for bacteriostatic with 0.2 μ m syringe filter filtration sterilization.
Embodiment 3
This experiment is measured bacterial growth amount in the tryptic soy broth (TSB) with inhibition test.Preparation is used for all the wart sphagnum mosses and the pappus sphagnum moss extract of this test as mentioned above.Also prepare TSB, autoclaving and be stored in 4 ℃ stand-by.Adopt following equipment:
A) Beckman  DU-64 spectrophotometer
B) incubator (available from Boekel Instruments Model#133000)
C) 5mL and 5OmL polystyrene test tube (available from Falcon)
D) 10 μ L and 1mL polypropylene tip (available from Pipetman)
Reagent and solution:
A) Bacto  tryptic soy broth (TSB), 30g/L (available from Becton Dickenson)
B) at grown at least intestinal bacteria (Escherichia Coli) the freezing cultivation mother liquors (clinical isolates) of 3 logarithmic phases of TSB
C) grown at least streptococcus aureus (Staphylococcus Aureus) of 3 logarithmic phases of TSB.(ATCC bacterial strain #29213 (American type culture collection, Manassas, VA))
D) distilled water (available from Premium Water Inc.)
E) the TSB extract of wart sphagnum moss and New Zealand's mosses
Step:
1) by 30g/L Bacto  tryptic soy broth being added preparation TSB nutrient broth in the distilled water.Stir this solution with stirring rod and dissolve, 121 ℃ of autoclavings 20 minutes up to all powder.The as above described preparation wart of B sphagnum moss extract.The TSB sample that 1mL solution, TSB or mosses are handled sucks 3-5mL polystyrene test tube.
2) intestinal bacteria that are stored in-20 ℃ and the freezing equal portions of streptococcus aureus are thawed to room temperature.In case after thawing, each 100 μ L adds among the TSB of 10mL equal portions with the bacterium mother liquor.Cover each test tube then, thorough mixing is marked and is put into 37 ℃ incubator.
3) 10 μ L bacterium mother liquors are sucked in the 1mL solution, this test tube is hatched required time at 37 ℃.Do not add bacterium in 1 test tube of each sample and time point, blank to be used as.
4) at the appointed time take out solution and put into ice bath o'clock from 37 ℃ of incubators.Then rapidly on spectrophotometer with 550nm to the sample reading.Absolute OD value is that sample subtracts blank.By measuring inhibition with respect to the reduction percentage ratio of suitable TSB control sample OD value.
Embodiment 4
Following data declaration is handled the effect of bacterial growth media (being tryptic soy broth) with various mosses according to the step of embodiment 3.Non-sphagnum moss kind is preventing that performance is very poor aspect the intestinal bacteria growth.In the mosses of following mensuration, can prevent most effectively that the sphagnum moss of intestinal bacteria growth from being wart sphagnum moss and pappus sphagnum moss." N " refers to testing time, and " scope " is the highest and minimum that these tests obtain; " mean value " refers to the mean value tested.
The sphagnum moss kind The % bacterial growth suppresses
Mean value N Scope
Wart bog moss (MN) pappus bog moss (NZ-Sutton Moss) intermediate peat moss (S.magellanicum) (WI-Mosser Lee) rust bog moss (S.fuscum) is (NZ) non-bog moss kind cypress shape folder tongue (Sheet Moss) (WI-Mosser Lee) iron orchid (Spanish Moss) (WI-Mosser Lee) of sickleleaf bog moss (S.falcatulum) (MN) 48 45 34 20 7 4 -2 10 8 7 1 2 1 1 26-71 31-62 21-43 -- 2-11 - -
Embodiment 5
Handle TSB with the wart sphagnum moss, measure the ability of this solution support bacteria growth according to embodiment 3.Reported that the bacterial growth to intestinal bacteria (clinical isolates) and streptococcus aureus (ATCC bacterial strain #29213) suppresses percentage ratio.Below report the TSB solution (MT-TSB) that TSB control sample and mosses are handled.The OD that the sample OD (measured value) that measures deducts blank (B) obtains reported values.
Test material Intestinal bacteria Streptococcus aureus
B Measured value Value B Measured value Value
TSB (contrast) mean value SD MT-TSB (5mg/mL) mean value SD % suppresses MT-TSB (10mg/mL) mean value SD % and suppresses MT-TSB (25mg/mL) mean value SD % inhibition 0.063 0.109 0.131 0.173 0.744 0.726 0.662 0.714 0.575 0.748 0.662 0.652 0.681 0.663 0.672 0.013 0.553 0.605 0.579 0.037 13.84 0.444 0.617 0.531 0.122 21.06 0.489 0.479 0.484 0.007 27.98 0.063 0.109 0.131 0.173 0.250 0.257 0.279 0.285 0.278 0.274 0.276 0.284 0.187 0.194 0.191 0.005 0.170 0.176 0.173 0.004 9.19 0.147 0.143 0.145 0.003 23.88 0.101 0.111 0.107 0.006 43.83
MT-TSB (50mg/mL) mean value SD % suppresses MT-TSB (75mg/mL) mean value SD % and suppresses MT-TSB (100mg/mL) mean value SD % inhibition 0.243 0.284 0.274 0.599 0.564 0.388 0.430 0.322 0.339 0.356 0.321 0.339 0.025 49.63 0.104 0.146 0.125 0.030 81.40 0.048 0.065 0.057 0.012 91.59 0.243 0.284 0.274 0.355 0.352 0.361 0.355 0.322 0.310 0.112 0.109 0.111 0.002 41.99 0.077 0.071 0.074 0.004 61.15 0.048 0.036 0.042 0.008 77.95
Embodiment 6
The present embodiment proof is not is not killed and wounded bacterium with the mosses processing but is suppressed its growth.Use Probes, Eugene, the L-7012 fluorometric assay kit measurement bacterium vigor of Oregon available from Molecular.This system adopts green and the red fluorescence nucleic acid dye mixture with the separating capacity that penetrates bacterium alive and dead bacterial cell.Green fluorescence dyestuff (emission wavelength 500nm) can be incorporated into bacterium alive and dead bacterium.Red fluorescence dyestuff (emission wavelength 650nm) only is incorporated into dead bacterium.Therefore, the dyeing ratio that contains the bacteria samples of more dead bacterium can change.Data presentation, for intestinal bacteria and streptococcus aureus, the bacterium that lives keeps identical with control sample with the ratio of dead bacterium.
Step:
1. 100 μ L (preparation of the control TSB of pH and wart sphagnum moss sample respectively as embodiment 1 and 2 as described in) 37 ℃ in the 10mL substratum hatched 3 hours.
2. centrifugal this mixture is resuspended in bacterium 10mL phosphate buffered saline (PBS) (PBS) then to concentrate bacterium.
3. respectively that 3mL is resuspended bacterium adds in three cuvettes.
4. 40 μ L Styo-9 dyestuffs are mixed with 40 μ L iodate, the third ingot dyestuff.Should be careful during use, because these compounds are considered to carcinogens.
5. 9 μ L blended dye solutions are added each cuvette, in the dark store 15 minutes.
6. prepare two and do not have the PBS cuvette of dyestuff and PBS cuvette dyestuff that two contain dyestuff as blank.
7. this solution of thorough mixing.Measure fluorescence intensity at 500nm (absorbing) and 650nm (absorbing) at the 490nm place at the 480nm place.The ratio of these two values is relevant with the vigor degree of bacterial cultures.
Following table has been reported the fluorescence intensity of bacteria samples under two wavelength in two kinds of different substratum.The fluorescence intensity of 500nm is relevant with the vigor degree of bacterial cultures divided by the ratio of the fluorescence intensity generation of 650nm.Reported the mean value of the TSB sample (MT-TSB) of three kinds of TSB and three kinds of mosses-processing below.Under each situation, will suppress percentage ratio and compare with control sample.
The vitality test of intestinal bacteria (clinical isolates)
The ratio of substratum 500nm 650nm 500/650
TSB 505.117 5.783 87.3228
The MT-TSB 130.1833 1.5167 85.8352 of wart sphagnum moss
The vitality test of streptococcus aureus (ATCC bacterial strain #29213)
The ratio of substratum 500nm 650nm 500/650
TSB 80.117 3.533 22.6745
The MT-TSB 45.055 2.0667 21.7984 of wart sphagnum moss
Data presentation, TSB does not have significance to change with the ratio of the TSB that mosses are handled, and the effect that mosses are described is antibacterial but not sterilization.
Embodiment 7
Handle various bacteriums according to embodiment 3 usefulness wart sphagnum moss TSB (concentration 50mg/mL).Reported that bacterial growth suppresses percentage ratio.Below report the TSB solution (MT-TSB) that TSB control sample and mosses are handled.Deduct optical density(OD) (OD) acquisition " value " of blank (B) by the mensuration OD (measured value) of sample.
Carried out twice research, hereinafter referred to as (1) and (2).
Test material (1) Intestinal bacteria (clinical isolates) Streptococcus aureus ATCC#29213
B Measured value Value B Measured value Value
TSB (contrast) mean value SD MT-TSB mean value SD % suppresses 0.034 0.219 0.694 0.718 0.542 0.572 0.660 0.684 0.672 0.017 0.323 0.353 0.338 0.021 49.7 0.034 0.219 0.273 0.257 0.362 0376 0.239 0.253 0.246 0.010 0.143 0.157 0.150 0.010 39.02
Test material (2) Intestinal bacteria Streptococcus aureus
B Measured value Value B Measured value Value
TSB (contrast) mean value SD MT-TSB mean value SD % suppresses 0.043 0.247 0.693 0.667 0.492 0.498 0.650 0.624 0.637 0.018 0.245 0.251 0.248 0.004 61.07 0.043 0.247 0.332 0.327 0.394 0.428 0.289 0.284 0.287 0.004 0.147 0.181 0.164 0.024 42.76
Test material (1) Staphylococcus epidermidis (S.epidermidis) ATCC#12228 Pseudomonas aeruginosa (P.aeruginosa) ATCC#10145
B Measured value Value B Measured value Value
TSB (contrast) mean value SD MT-TSB mean value SD % suppresses 0.034 0.219 0.388 0.412 0.542 0.572 0.354 0.375 0.366 0.017 0.323 0.353 0.338 0.021 53.26 0.034 0.219 0.175 0.167 0.321 0.331 0.141 0.133 0.137 0.006 0.102 0.112 0.107 0.007 21.90
Test material (2) Staphylococcus epidermidis Pseudomonas aeruginosa
B Measured value Value B Measured value Value
TSB (contrast) mean value SD MT-TSB mean value SD % suppresses 0.043 0.247 0.349 0.327 0.428 0.444 0.306 0.284 0.295 0.016 0.181 0.197 0.189 0.011 35.93 0.043 0.247 0.204 0.186 0.371 0.340 0.161 0.143 0.152 0.013 0.124 0.093 0.109 0.022 28.62
Test material (1) Candida albicans (C.albicans) ATCC#10231 A.amsterodami ATCC#1001
B Measured value Value B Measured value Value
TSB (contrast) mean value SD MT-TSB mean value SD % suppresses 0.034 0.219 0.068 0.069 0.249 0.245 0.034 0.035 0.035 0.001 0.030 0.026 0.028 0.003 18.84 0.034 0.219 0.053 0.047 0.231 0.229 0.019 0.013 0.016 0.004 0.012 0.010 0.011 0.001 31.25
Test material (2) Candida albicans A.amsterodami
B Measured value Value B Measured value Value
TSB (contrast) mean value SD MT-TSB mean value SD % suppresses 0.043 0.247 0.1019 0.057 0.275 0.292 0.058 0.044 0.051 0.010 0.028 0.045 0.037 0.012 28.43 0.043 0.247 0.067 0.071 0.268 0.254 0.024 0.028 0.026 0.003 0.021 0.007 0.014 0.010 46.15
The embodiment 8--effect of acid treatment mosses
With the compressed rod of pappus sphagnum moss (available from Sutton ' s Moss, Canada (collecting)) immerses four kinds of distilled water solution (Fe standard substance in the concentrated hydrochloric acid that Fe concentration increases gradually in New Zealand, 0,0.5,5,50mg/L, available from RiccaChemicals, Arlington, Texas) in, making mosses concentration is 50mg/ml.The mosses that to soak into are 4 ℃ of store overnight.With the syringe Fe solution of squeezing out, filter, with inducing link coupled plasma atomic emission spectrum assay method assay determination Fe.The result shows that mosses combine the Fe of significant quantity, in the 50mg/L sample up to 15mg/L.Undertaken this experiment by the distilled water wash mosses, obtain analog result.Yet, notice that (spiked) sample pH value of Fe reinforcement is low.Owing to realize optimum combination when pH 4-6, we heighten 4-7 with pH in the next round test.When the pH of Fe sample raise, Fe began precipitation.Mosses have been removed all Fe (up to 25mg/L) in the sample.Determine that then the ion that is incorporated into mosses before usefulness can influence positively charged ion in conjunction with test, should study the cationic method of removing in the mosses.
In the method for exploitation acid elution peat mosses (be not peat moss, peat moss is septic mosses), two kinds of different acid have at first been used.The a collection of mosses of continuously stirring (about 5g) are used dense HNO in 3.5L distilled water 3PH is adjusted into 1.For the mosses of other batch, adopt 2% glacial acetic acid solution.Agitator treating thing 1 hour filters supernatant then, and new washing soln is added mosses.Repeat acid elution altogether four times.Behind the acid elution, use distilled water wash in the same manner 4 times.When distilled water wash finished, its specific conductivity was similar to distilled water.The ionic weight of these two kinds acid removals is similar; Therefore generally washed mosses afterwards with acetate.
Determined under neutral pH that soluble metal is more suitable for measuring binding capacity and mensuration improve with acid elution peat mosses bonded (with respect to do not wash combining of mosses).Select calcium to be used for this purpose.Used mosses are present embodiment described pappus sphagnum mosses before, and used acid is 2% glacial acetic acid solution, washs as described in the previous paragraph.First test shows opposite with expectation, and it is maximum not wash the mosses bonded, and the mosses bonded of acid elution is minimum.When sample for reference pH, find that binding ability is relevant with pH.The mosses of acid elution have reduced the pH of solution, and this can influence ionic bond.For eliminating this problem, the mosses of using the washing of high level salt solution washing acid are to replace the H+ ion with Na.High level salt solution is the 1M sodium acetate, continues stir about 5g mosses 1 hour, repeated washing then in this kind of 3.5L sodium acetate solution.After this twice sodium acetate solution washing, use distilled water wash 4 times, washed 1 hour at every turn, each washing is carried out in the same manner.Measure the mosses of acid/salt washing; The pH of press juice does not reduce, and the combination that demonstrates than distilled water wash and unwashed mosses significantly improves (Ca concentration is represented with ppm) as shown in the table.
Mosses are handled Initial [Ca] Average [Ca] that obtains Standard deviation pH
Contrast acetic acid, acetate do not wash distilled water washing acetic acid, acetate and do not wash distilled water washing contrast acetic acid, acetate and do not wash distilled water and wash and contrast 0 0 0 0 100 100 100 100 200 200 200 200 -1.08 -0.97 -0.14 0.36 -1.40 11.24 16.89 88.76 -0.33 46.47 53.55 198.10 0.61 0.45 0.51 1.32 0.25 0.00 1.17 0.05 0.87 5.88 4.42 1.83 6.02 7.62 5.26 5.13 6.62 4.81 4.54 6.65 6.17 4.82 4.61 6.76
Therefore the variation of extract pH still is difficult to distinguish the effect of acid elution and pH up to 2 pH units.Therefore developed mensuration mosses bonded novel method, it can still adjust pH under the situation in sample solution in mosses.Finish this method with porous plate, little stirring rod and 10mg/ml mosses concentration.Solution is on the mosses approximately after half an hour, the pH of all solution adjusted in 0.25 pH unit of 6.5.The mosses that this can measure the mosses of acid/salt washing and only use acid elution.The result is as follows.Shown in mean value be the mean value of two samples.
Mosses are handled Initial [Ca] Average [Ca] that obtains Standard deviation
Not not washing contrast acetic acid/not washing contrast of sodium acetate washing acetic acid washing distilled water washing of washing contrast acetic acid/sodium acetate washing acetic acid washing distilled water washing of acetic acid/sodium acetate washing acetic acid washing distilled water washing 0.00 0.00 0.00 0.00 0.00 100.00 100.00 100.00 100.00 100.00 500.00 500.00 500.00 500.00 500.00 6.93 6.16 6.85 8.54 8.10 6.78 6.82 20.73 26.25 92.27 307.28 319.25 375.18 382.85 454.30 0.72 0.16 0.31 1.56 0.31 0.31 0.26 1.09 0.72 1.56 7.25 8.13 3.36 4.38 8.91
Because pH adjusts to mutual difference in 0.5 pH unit; Eliminated the pH effect, the binding capacity that can be observed the mosses of acid elution significantly improves.The binding ratio of the mosses of acid/salt washing only uses the mosses of acid elution good slightly, distilled water wash better slightly than unwashed mosses, but be enough to hardly be called significantly.
Therefore, prove that therefore mosses can be effective to water treatment in conjunction with Fe and Ca ion, one or both are purposes of water treatment because remove these two kinds of ionic.
The purpose that above-mentioned specification sheets and accompanying drawing are provided is to describe embodiment of the present invention, and is not intended to limit by any way the scope of the invention.It will be understood by those skilled in the art that and to carry out various modifications and change, and do not deviate from the spirit or scope of the present invention.Therefore, the present invention is intended to cover modification of the present invention and change, if they enter the scope of the claims and the equivalent form of value thereof.

Claims (186)

1. method that suppresses microorganism growth, described method comprise the material of microorganism that is easy to grow are contacted with the mosses of rotting of the amount that effectively suppresses microorganism growth that wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
2. the method for claim 1 is characterized in that, described mosses are wart sphagnum mosses.
3. the method for claim 1 is characterized in that, described mosses are pappus sphagnum mosses.
4. the method for claim 1 is characterized in that, described mosses only comprise the wart sphagnum moss.
5. the method for claim 1 is characterized in that, described mosses only comprise the pappus sphagnum moss.
6. the method for claim 1 is characterized in that, described mosses only comprise the mixture of wart sphagnum moss and pappus sphagnum moss.
7. the method for claim 1 is characterized in that, described mosses are compressed.
8. method as claimed in claim 7 is characterized in that described mosses are bar shapeds.
9. the method for claim 1 is characterized in that, described mosses are through germicidal treatment.
10. the method for claim 1 is characterized in that, described mosses are washed through acidic solution.
11. method as claimed in claim 10 is characterized in that, described mosses are washed through acetic acid solution.
12. method as claimed in claim 10 is characterized in that, described mosses are washed with salts solution after the acidic solution washing.
13. the method for claim 1 is characterized in that, described material is a water.
14. the method for claim 1 is characterized in that, described material is the water among the Spa.
15. the method for claim 1 is characterized in that, described material is the water in the pool.
16. the method for claim 1 is characterized in that, described material is the water in the fishpond.
17. the method for claim 1 is characterized in that, described material is the part of human body.
18. method as claimed in claim 17 is characterized in that, the part of described human body is a skin.
19. method as claimed in claim 17 is characterized in that, the part of described human body is a surface wound.
20. method as claimed in claim 17 is characterized in that, the part of described human body is an inner coelom.
21. method as claimed in claim 17 is characterized in that, the part of described human body is the internal injury position.
22. the method for claim 1 is characterized in that, described material is a cereal.
23. method that suppresses microorganism growth, described method comprises to be put into the mosses of rotting that effectively suppress the amount of microorganism growth load bearing component and the material of described load bearing component with the microorganism that is easy to grow is contacted, and wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
24. method as claimed in claim 23 is characterized in that, described mosses are wart sphagnum mosses.
25. method as claimed in claim 23 is characterized in that, described mosses are pappus sphagnum mosses.
26. method as claimed in claim 23 is characterized in that, described mosses only comprise the wart sphagnum moss.
27. method as claimed in claim 23 is characterized in that, described mosses only comprise the pappus sphagnum moss.
28. method as claimed in claim 23 is characterized in that, described mosses only comprise the mixture of wart sphagnum moss and pappus sphagnum moss.
29. method as claimed in claim 23 is characterized in that, described material is a water.
30. method as claimed in claim 23 is characterized in that, described material is the water among the Spa.
31. method as claimed in claim 23 is characterized in that, described material is the water in the pool.
32. method as claimed in claim 23 is characterized in that, described material is the water in the fishpond.
33. method as claimed in claim 29 is characterized in that, described water pumps via described load bearing component.
34. method as claimed in claim 23 is characterized in that, described material is the part of human body.
35. method as claimed in claim 34 is characterized in that, the part of described human body is a skin.
36. method as claimed in claim 34 is characterized in that, the part of described human body is a surface wound.
37. method as claimed in claim 34 is characterized in that, the part of described human body is an inner coelom.
38. method as claimed in claim 34 is characterized in that, the part of described human body is the internal injury position.
39. method as claimed in claim 23 is characterized in that, described material is a cereal.
40. method as claimed in claim 23 is characterized in that, described load bearing component is the device of implant into body.
41. method as claimed in claim 23 is characterized in that, described load bearing component is a polymeric matrix.
42. method as claimed in claim 23 is characterized in that, described load bearing component is a biological substrate.
43. method as claimed in claim 23 is characterized in that, described load bearing component is one or more layers film.
44. a method for preparing the mosses that are used for bacteria growing inhibiting, described method comprises:
(i) make unputrefied mosses drying; With
(ii) described mosses are sterilized.
45. method as claimed in claim 44 also comprises the compression mosses.
46. method as claimed in claim 45 also comprises the mosses of compression is cut into inch strips.
47. method as claimed in claim 44 is characterized in that, described mosses are through autoclaving.
48. method as claimed in claim 44 is characterized in that, described mosses are sterilized by chemical treatment.
49. method as claimed in claim 44 is characterized in that, described mosses are to sterilize by ethylene oxide treatment.
50. a method for preparing the mosses that are used for bacteria growing inhibiting, described method comprises:
(i) mosses of will not rotting contact with acidic solution; With
(ii) make described mosses drying.
51. method as claimed in claim 50 is characterized in that, after step (i) described unputrefied mosses is contacted with salts solution.
52. method as claimed in claim 51 is characterized in that, described acidic solution is an acetic acid solution.
53. method as claimed in claim 50 is characterized in that, described mosses are peat mosses.
54. method as claimed in claim 50 is characterized in that, described mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
55. method as claimed in claim 54 is characterized in that, described mosses are wart sphagnum mosses.
56. method as claimed in claim 54 is characterized in that, described mosses are pappus sphagnum mosses.
57. method as claimed in claim 54 is characterized in that, described mosses only comprise the wart sphagnum moss.
58. method as claimed in claim 54 is characterized in that, described mosses only comprise the pappus sphagnum moss.
59. method as claimed in claim 54 is characterized in that, described mosses only comprise the mixture of wart sphagnum moss and pappus sphagnum moss.
60. a kit, described kit comprise sterilized mosses and the load bearing component of not rotting.
61. kit as claimed in claim 60 is characterized in that, described mosses are peat mosses.
62. kit as claimed in claim 60 is characterized in that, described mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
63. kit as claimed in claim 62 is characterized in that, described mosses are wart sphagnum mosses.
64. kit as claimed in claim 62 is characterized in that, described mosses are pappus sphagnum mosses.
65. kit as claimed in claim 62 is characterized in that, described mosses only comprise the wart sphagnum moss.
66. kit as claimed in claim 62 is characterized in that, described mosses only comprise the pappus sphagnum moss.
67. kit as claimed in claim 62 is characterized in that, described mosses only comprise the mixture of wart sphagnum moss and pappus sphagnum moss.
68. kit as claimed in claim 60 is characterized in that, described mosses are compressed.
69., it is characterized in that described mosses are bar shapeds as the described kit of claim 68.
70. kit as claimed in claim 60 is characterized in that, described mosses are through germicidal treatment.
71. kit as claimed in claim 60 is characterized in that, described mosses are washed through acidic solution.
72., it is characterized in that described mosses are washed through acetic acid solution as the described kit of claim 71.
73., it is characterized in that described mosses are washed with salts solution as the described kit of claim 71 after the acidic solution washing.
74. the method for a bacteria growing inhibiting, described method comprises the water of the bacterium that is easy to grow is contacted with the effective mosses of rotting of the amount of bacteria growing inhibiting, with regularly impact water with suitable chemical substance, wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
75., it is characterized in that described chemical substance is a potassium hydrogen persulfate as the described method of claim 74.
76. a water treatment method, described method comprise with water with effectively remove that the rotten mosses of the cationic amount except that hydrogen ion contact in the water, wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
77., it is characterized in that described positively charged ion is a calcium ion as the described method of claim 76.
78. as the described method of claim 77, it is characterized in that, removed all calcium ions in the water basically.
79., it is characterized in that described positively charged ion is an iron ion as the described method of claim 76.
80. as the described method of claim 79, it is characterized in that, removed all calcium ions in the water basically.
81., it is characterized in that described mosses are wart sphagnum mosses as the described method of claim 76.
82., it is characterized in that described mosses are pappus sphagnum mosses as the described method of claim 76.
83., it is characterized in that described mosses only comprise the wart sphagnum moss as the described method of claim 76.
84., it is characterized in that described mosses only comprise the pappus sphagnum moss as the described method of claim 76.
85., it is characterized in that described mosses only comprise the mixture of wart sphagnum moss and pappus sphagnum moss as the described method of claim 76.
86., it is characterized in that described mosses are compressed as the described method of claim 76.
87., it is characterized in that described mosses are bar shapeds as the described method of claim 86.
88., it is characterized in that described mosses are through germicidal treatment as the described method of claim 76.
89., it is characterized in that described mosses are washed through acidic solution as the described method of claim 76.
90., it is characterized in that described mosses are washed through acetic acid solution as the described method of claim 89.
91., it is characterized in that described mosses are washed with salts solution as the described method of claim 89 after the acidic solution washing.
92., it is characterized in that described material is the water among the Spa as the described method of claim 76.
93., it is characterized in that described material is the water in the pool as the described method of claim 76.
94., it is characterized in that described material is the water in the fishpond as the described method of claim 76.
95. water treatment method, described method comprises to be put into load bearing component and described load bearing component is contacted with water effectively removing in the water except that hydrogen ion the mosses of rotting of cationic amount, and wherein said mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof.
96. a device that uses in water, described device comprises:
(i) has the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; With
Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses.
97., it is characterized in that described load bearing component comprises float as the described device of claim 96.
98., it is characterized in that described load bearing component comprises the column part under float and the float as the described device of claim 96, described column part has inner chamber and one or more permission flows into and the opening of outflow inner chamber.
99., it is characterized in that described mosses are contained in the mesh bag as the described device of claim 96.
100., it is characterized in that described load bearing component comprises one or more counterweights as the described device of claim 96.
101., it is characterized in that described mosses are unputrefied mosses as the described device of claim 96.
102., it is characterized in that described mosses are peat mosses as the described device of claim 101.
103., it is characterized in that described mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof as the described device of claim 101.
104., it is characterized in that described mosses are wart sphagnum mosses as the described device of claim 103.
105., it is characterized in that described mosses are pappus sphagnum mosses as the described device of claim 103.
106., it is characterized in that described mosses only comprise the wart sphagnum moss as the described device of claim 103.
107., it is characterized in that described mosses only comprise the pappus sphagnum moss as the described device of claim 103.
108., it is characterized in that described mosses only comprise the mixture of wart sphagnum moss and pappus sphagnum moss as the described device of claim 103.
109., it is characterized in that described mosses are compressed as the described device of claim 101.
110., it is characterized in that described mosses are bar shapeds as the described device of claim 109.
111., it is characterized in that described mosses are through germicidal treatment as the described device of claim 101.
112., it is characterized in that described mosses are washed through acidic solution as the described device of claim 101.
113., it is characterized in that described mosses are washed through acetic acid solution as the described device of claim 101.
114., it is characterized in that described mosses are washed with salts solution as the described device of claim 101 after the acidic solution washing.
115. a method that suppresses microorganism growth, described method comprise the water of the device that suppresses microorganism growth in the water being put into the microorganism that is easy to grow, described device comprises:
(i) has the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; With
Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises the mosses of the amount of microorganism growth in effective inhibition water.
116., it is characterized in that described load bearing component comprises float as the described method of claim 115.
117., it is characterized in that described load bearing component comprises the column part under float and the float as the described method of claim 115, described column part has inner chamber and one or more permission flows into and the opening of outflow inner chamber.
118., it is characterized in that described load bearing component comprises one or more counterweights as the described method of claim 115.
119., it is characterized in that described mosses are contained in the mesh bag as the described method of claim 115.
120., it is characterized in that described mosses are unputrefied mosses as the described method of claim 115.
121., it is characterized in that described mosses are peat mosses as the described method of claim 115.
122., it is characterized in that described mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof as the described method of claim 120.
123., it is characterized in that described mosses are wart sphagnum mosses as the described method of claim 122.
124., it is characterized in that described mosses are pappus sphagnum mosses as the described method of claim 122.
125., it is characterized in that described mosses only comprise the wart sphagnum moss as the described method of claim 122.
126., it is characterized in that described mosses only comprise the pappus sphagnum moss as the described method of claim 122.
127., it is characterized in that described mosses only comprise the mixture of wart sphagnum moss and pappus sphagnum moss as the described method of claim 122.
128., it is characterized in that described mosses are compressed as the described method of claim 115.
129., it is characterized in that described mosses are bar shapeds as the described method of claim 128.
130., it is characterized in that described mosses are through sterilising treatment as the described method of claim 120.
131., it is characterized in that described mosses are washed through acidic solution as the described method of claim 120.
132., it is characterized in that described mosses are washed through acetic acid solution as the described method of claim 120.
133., it is characterized in that described mosses are washed with salts solution as the described method of claim 120 after the acidic solution washing.
134., it is characterized in that described water is the water among the Spa as the described method of claim 120.
135., it is characterized in that described water is the water in the pool as the described method of claim 120.
136., it is characterized in that described water is the water in the fishpond as the described method of claim 120.
137., it is characterized in that described water pumps via described load bearing component as the described method of claim 120.
138. a kit that comprises the device that does not rot mosses and use of sterilization in water, described device comprises the load bearing component that has inner chamber and one or more permission inflow and flow out the opening of inner chamber, and wherein said inner chamber can be sealed the residence fully and state mosses.
139., it is characterized in that described load bearing component comprises float as the described kit of claim 138.
140., it is characterized in that described load bearing component comprises the column part under float and the float as the described kit of claim 138, described column part has inner chamber and one or more permission flows into and the opening of outflow inner chamber.
141., it is characterized in that described load bearing component comprises one or more counterweights as the described kit of claim 138.
142., it is characterized in that described mosses are contained in the mesh bag as the described kit of claim 138.
143., it is characterized in that described mosses are peat mosses as the described kit of claim 138.
144., it is characterized in that described mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof as the described kit of claim 138.
145., it is characterized in that described mosses are wart sphagnum mosses as the described kit of claim 144.
146., it is characterized in that described mosses are pappus sphagnum mosses as the described kit of claim 144.
147., it is characterized in that described mosses only comprise the wart sphagnum moss as the described kit of claim 144.
148., it is characterized in that described mosses only comprise the pappus sphagnum moss as the described kit of claim 144.
149., it is characterized in that described mosses only comprise the mixture of wart sphagnum moss and pappus sphagnum moss as the described kit of claim 144.
150., it is characterized in that described mosses are compressed as the described kit of claim 138.
151., it is characterized in that described mosses are bar shapeds as the described kit of claim 150.
152., it is characterized in that described mosses are through germicidal treatment as the described kit of claim 138.
153., it is characterized in that described mosses are washed through acidic solution as the described kit of claim 138.
154., it is characterized in that described mosses are washed through acetic acid solution as the described kit of claim 138.
155., it is characterized in that described mosses are washed with salts solution as the described kit of claim 138 after the acidic solution washing.
156., also comprise one or more pH calibration tapes as the described kit of claim 138.
157., also comprise potassium hydrogen persulfate as the described kit of claim 138.
158. a method that suppresses microorganism growth, described method comprise the device that suppresses microorganism growth in the water is put into the water of microorganism and regularly impact water with suitable chemical substance of being easy to grow, described device comprises:
(i) has the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; With
Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises the mosses of the amount of microorganism growth in effective inhibition water.
159., it is characterized in that described chemical substance is a potassium hydrogen persulfate as the described method of claim 158.
160. a water treatment method, described method comprise a kind of device is put into water, described device comprises:
(i) has the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; With
Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises in effective removal water the described mosses of cationic amount except that hydrogen ion.
161., it is characterized in that described positively charged ion is a calcium ion as the described method of claim 160.
162. as the described method of claim 161, it is characterized in that, removed all calcium ions in the water basically.
163., it is characterized in that described positively charged ion is an iron ion as the described method of claim 160.
164. as the described method of claim 163, it is characterized in that, removed all calcium ions in the water basically.
165., it is characterized in that described load bearing component comprises float as the described method of claim 160.
166., it is characterized in that described load bearing component comprises the column part under float and the float as the described method of claim 160, described column part has inner chamber and one or more permission flows into and the opening of outflow inner chamber.
167., it is characterized in that described load bearing component comprises one or more counterweights as the described method of claim 160.
168., it is characterized in that described mosses are contained in the mesh bag as the described method of claim 160.
169., it is characterized in that described mosses are unputrefied mosses as the described method of claim 160.
170., it is characterized in that described mosses are peat mosses as the described method of claim 160.
171., it is characterized in that described mosses are selected from wart sphagnum moss, pappus sphagnum moss and composition thereof as the described method of claim 160.
172., it is characterized in that described mosses are wart sphagnum mosses as the described method of claim 171.
173., it is characterized in that described mosses are pappus sphagnum mosses as the described method of claim 171.
174., it is characterized in that described mosses only comprise the wart sphagnum moss as the described method of claim 171.
175., it is characterized in that described mosses only comprise the pappus sphagnum moss as the described method of claim 171.
176., it is characterized in that described mosses only comprise the mixture of wart sphagnum moss and pappus sphagnum moss as the described method of claim 171.
177., it is characterized in that described mosses are compressed as the described method of claim 160.
178., it is characterized in that described mosses are bar shapeds as the described method of claim 177.
179., it is characterized in that described mosses are through germicidal treatment as the described method of claim 169.
180., it is characterized in that described mosses are washed through acidic solution as the described method of claim 169.
181., it is characterized in that described mosses are washed through acetic acid solution as the described method of claim 169.
182., it is characterized in that described mosses are washed with salts solution as the described method of claim 169 after the acidic solution washing.
183., it is characterized in that described water is the water among the Spa as the described method of claim 160.
184., it is characterized in that described water is the water in the pool as the described method of claim 160.
185., it is characterized in that described water is the water in the fishpond as the described method of claim 160.
186., it is characterized in that described water pumps via described load bearing component as the described method of claim 160.
CN2005800115381A 2004-04-14 2005-04-14 Methods of inhibiting microorganism growth using moss and devices for water treatments Expired - Fee Related CN1942405B (en)

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US4002566A (en) * 1975-04-07 1977-01-11 Smith John D Water purifying systems
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CN1017868B (en) * 1984-07-16 1992-08-19 庄臣及庄臣有限公司 Antimicrobial peat moss composition
US4618496A (en) * 1984-07-16 1986-10-21 Johnson & Johnson Antimicrobial peat moss composition
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CA2188198C (en) * 1995-10-19 2004-04-06 Stephen G. Simmering Peat bale filtration element
US6620321B2 (en) * 1999-12-30 2003-09-16 Edward R. Festa Biofilter system and method for treating wastewater
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