CN1942405B - Methods of inhibiting microorganism growth using moss and devices for water treatments - Google Patents
Methods of inhibiting microorganism growth using moss and devices for water treatments Download PDFInfo
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- CN1942405B CN1942405B CN2005800115381A CN200580011538A CN1942405B CN 1942405 B CN1942405 B CN 1942405B CN 2005800115381 A CN2005800115381 A CN 2005800115381A CN 200580011538 A CN200580011538 A CN 200580011538A CN 1942405 B CN1942405 B CN 1942405B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/286—Treatment of water, waste water, or sewage by sorption using natural organic sorbents or derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/42—Nature of the water, waste water, sewage or sludge to be treated from bathing facilities, e.g. swimming pools
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Abstract
The invention provides a method of inhibiting microorganism growth comprising contacting a substance susceptible to microorganism growth with an amount of a non-decomposed moss effective to inhibit microorganism growth, wherein the moss is selected from the group consisting of sphagnum papillosum, sphagnum cristatum, and mixtures therof. The invention provides a device (10) for use in water comprising a carrier having an interior cavity (32) and one or more openings (38, 35) allowing ingress to and egress from the interior cavity (32); and a moss contained within the interior cavity (32) in which the carrier completely encloses the moss.
Description
Invention field
The present invention relates to adopt mosses, specifically be method and the method for treating water that the peat mosses suppress growth of microorganism.
Background of invention
The known various BIOLOGICAL CONTROL materials in growth of microorganism control field.For example, need in food, suppress conk when storing as cereal, and need be at sheathing foodstuff, as bacteria growing inhibiting in the live chickens meat.Absorb in the product such as bandage or feminine hygiene of liquid at other, need comprise antimicrobial compositions so that pathogenic biology can not be bred in a large number.
Water treatment is another field of controlling or prevent bacterial growth with chemistry, biology and treatment with irradiation.The water treatment system that many types are arranged is as the filtration and the cleaning systems in swimming pool and fishpond.Many these system filtration water are to remove suspension and to reduce the muddy phenomenon of water.Bacterial growth in the anti-sealing and the pollutant of removing in the water are important industry and family problems.For example, answer the cleaned industry waste liquid to remove toxic compounds be discharged in lake and the river at it before and to remove bacterium.Water receptacle such as swimming pool, hot tub, fishpond etc. must keep clean to prevent that water from becoming muddiness and/or chamber wall becomes sticky.Available active mode such as filter are handled water, and to remove particle and bacterium, also available passive mode is handled water as antimicrobial being put into container and being floated over water.
Usually use chemical mode to keep the cleaning and the minimizing bacterial growth of water.Can adopt ultraviolet ray, chlorine processing, bromine processing, copper and silver ion treatment and ozone treatment is handled and/or disinfectant.These are typical antimicrobials, promptly destroy material or the energy of living organism.Certainly, must be careful when using all these methods, because they are may be to the user poisonous or damage arranged.Chemical substance needs handled, to avoid contaminated environment and contact user.
" peat mosses " (" Sphagnum moss ") is a kind of general name, is illustrated in a class floristics that coexists in the sphagnum bog.It should be noted that " peat moss (peat moss) " is often referred to the peat mosses of rotting or being used for compost.Usually collect the peat mosses and be used for various products.The preferred hat (petal) of collecting these mosses, but not stem.The general plant corpus (root, branch etc.) of removing bulk can further be processed mosses after the collection, promptly form aqueous slurry with the very thin particle of extracting.Remove the water in the slurries, make the mosses drying.Compressible mosses before packing or transportation.Available various additive changes the absorption characteristic or the mechanical property of mosses.Because the peat mosses are easy to obtain, and relatively inexpensive,, be mainly used in absorption liquid so it has been used for various products.
This area is starved of the product that suppresses microorganism such as bacterium, yeast and algal grown.Need a kind of mode and keep water clarification in swimming pool, whirlpool bath, the fishpond etc. for a long time, clean and need not shutdown system.The system that wants most only needs considerably less maintenance and relatively cheap.
Summary of the invention
The invention provides a kind of method that suppresses growth of microorganism, described method comprises the material of the microorganism that is easy to grow is contacted with the mosses of rotting of the amount that effectively suppresses growth of microorganism that wherein said mosses are selected from wart bog moss, pappus bog moss and composition thereof.
The invention provides a kind of method that suppresses growth of microorganism, described method comprises to be put into the mosses of rotting that effectively suppress the amount of growth of microorganism load bearing component and the material of described load bearing component with the microorganism that is easy to grow is contacted, and wherein said mosses are selected from wart bog moss, pappus bog moss and composition thereof.
The invention provides the method that a kind of preparation is used to suppress the mosses of growth of microorganism, described method comprises: (i) make unputrefied mosses drying; (ii) described mosses are sterilized.The invention provides the method that a kind of preparation is used for the mosses of bacteria growing inhibiting, described method comprises: (i) unputrefied mosses are contacted with acid solution; (ii) make described mosses drying.The invention provides a kind of kit that comprises sterilized do not rot mosses and load bearing component.
The invention provides a kind of method that suppresses growth of microorganism, described method comprises the water of the microorganism that is easy to grow is contacted with the mosses of rotting of the amount that effectively suppresses growth of microorganism, with regularly impact water with suitable chemical substance, wherein said mosses are selected from wart bog moss, pappus bog moss and composition thereof.
The invention provides a kind of method for treating water, described method comprise with water with effectively remove in the water that the rotten mosses of cationic amount contact except that hydrogen ion, wherein said mosses are selected from wart bog moss, pappus bog moss and composition thereof.The present invention also provides a kind of method for treating water, described method comprises to be put into load bearing component and described load bearing component is contacted with water effectively removing in the water except that hydrogen ion the mosses of rotting of cationic amount, and wherein said mosses are selected from wart bog moss, pappus bog moss and composition thereof.
The invention provides the device that uses in water, described device comprises: (i) have the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses.
The invention provides a kind of method that suppresses growth of microorganism, described method comprises the water of the device that suppresses growth of microorganism in the water being put into the bacterium that is easy to grow, and described device comprises: (i) have the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises the mosses of the amount of growth of microorganism in effective inhibition water.
The invention provides the kit that comprises the sterilized device that does not rot mosses and in water, use, described device comprises the load bearing component that has inner chamber and one or more permission inflow and flow out the opening of inner chamber, and wherein said inner chamber is sealed the residence fully and stated mosses.
The invention provides a kind of method that suppresses growth of microorganism, described method comprises the water of the device that suppresses growth of microorganism in the water being put into the microorganism that is easy to grow, with regularly impact water with suitable chemical substance, described device comprises: (i) have the load bearing component that inner chamber and one or more permission flow into and flow out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises the mosses of the amount of growth of microorganism in effective inhibition water.
The invention provides a kind of method for treating water, described method comprises puts into water with a device, and described device comprises: (i) have the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises in effective removal water the described mosses of cationic amount except that hydrogen ion.
Should be understood that above-mentioned general description and following detailed description all are exemplary and indicative, be intended to the present invention of prescription is further explained.
Brief Description Of Drawings
Figure 1A has illustrated the perspective view that can be used for an embodiment of the invention.Figure 1B has illustrated end view, and Fig. 1 C has illustrated along the sectional view of the C-C line of Figure 1B.
Fig. 2 A has illustrated the perspective view that can be used for another embodiment of the invention.Fig. 2 B has shown the end view of the mosses that are used for Fig. 2 A shown device.
Fig. 3 has illustrated the perspective view that can be used for another embodiment of the invention.
Preferred implementation describes in detail
We have found to can be used for suppressing the bog moss kind of growth of microorganism.Available these mosses controls are as the growth of microorganism in the water such as swimming pool, Spa, fishpond.Spa be also referred to as the spiral flow type bathing pool (rotary bathtub, whirlpool) or hot tub.The mosses of believing particular types are to suppressing and/or preventing that bacterium and other growth of microorganism are effective especially.
In the present invention, " bacteriostatic agent " refers to the material of bacteria growing inhibiting.In universaling dictionary, term " antibacterial agent " is often referred to the bacterial growth inhibitor.These two terms and " bactericide " should be distinguished, can kill and wound the material of bacterium during the bactericide abutment.
In the present invention, " water treatment " instigate that water in swimming pool, fishpond, bathtub of rotational-flow type, the hot tub etc. keeps clean, clarification and the pleasant technology of smell.When agitate water, it is less to observe foam.Believe that mosses can suppress bacterium and other growth of microorganism, the compound and the material of its also adsorbable reduction clarification of water degree.
In the present invention, the wart bog moss (sphagnum papillosum, S.papillosum) and/or the pappus bog moss (sphagnum.cristatum S.cristatum) can be used for water treatment facilities.In a preferred embodiment, mosses are installed to or are encapsulated into prevent in the Web materials that mosses disperse in water environment.Therefore, mosses can be remained in the desired location in pond, hot tub, the bathtub of rotational-flow type etc.Preferred Web materials comprises and contains polymer such as nylon or polyacrylic material, the about 0.1-1mm of sizing grid.Common preferred polymers is because their cheap and degradation resistants.
Be suitable for of the present inventionly being the wart bog moss that from the marsh in the north, Minn., to collect and can be used as compressed package from Sutton ' s Moss of Dobson, Westland, the pappus bog moss that New Zealand buys.These mosses kinds can be separately or one are used from device of the present invention or system.General and preferably, the cleaning mosses are to remove granule such as dirt and big fragment such as root and leaf.Commercially available mosses may be handled through sootiness before the manufacturer packs, to destroy seed.In a preferred embodiment, with mechanical device mosses are cut into required size and shape.Then, preferably by the HCS method, be exposed to oxirane or alternate manner well known by persons skilled in the art is sterilized to mosses.Sterilization process has destroyed the living organism in the mosses, thereby prevents to produce in the environment of any bad problem or alien bacteria introducing use apparatus of the present invention.Then, these mosses promptly can be used for water treatment system or other application.
We find that a kind of convenience, easy, effective and cheap water treatment mode are that a part of wart bog moss or pappus bog moss are put into the flotation gear that allows water to center on and flow through mosses.The another kind of mode of using it is to be encapsulated in it in the net and to increase the weight of this net, and mosses are stayed in the water.Any suitable method that keeps these mosses to contact with water all is fit to use.Then this device is placed in swimming pool, rotary bathtub, the hot tub etc., can contacts with water at these local devices.We find, this processing prevent aspect bacterial growth and maintenance water cleaning, clarification and tasteless and the non-foam effective especially.Because with impelling water to compare this processing by the filtration system of mosses is passive system, so effect is more unusual.Will of course be appreciated that available apparatus of the present invention are carried out the active filtration and handled water.
When being used for swimming pool, hot tub etc., water treatment facilities described herein preferably with the material coupling that kills and wounds bacterium.This is because these environment may contain a large amount of bacterium load that different time is introduced.Therefore, standard practices be as required filtered water, be filled with water to waterline and test water as required.Available commercial solution is adjusted pH.Water treatment facilities of the present invention best and oxidant such as potassium hydrogen persulfate coupling are called " no chlorine impacts ".Known potassium hydrogen persulfate can improve the efficient of chlorine purified product, but we are effective equally especially when finding to use it and above-mentioned peat mosses device coupling.
Peat mosses of the present invention can be used for any composition, material, device or the method that needs suppress microorganism.Application comprises the inhibition growth of microorganism, reduces and/or prevents peculiar smell, water treatment, control mould and conk; And control fermentation.This device and material comprise absorbent products, as in the diaper, feminine hygiene, bandage and wound dressing.In these products, these mosses can be packed between the different film of liquid transmission characteristic.Promptly (for example) skim can be that liquid is saturable, and another tunic can be a vapor permeable.These mosses can be mixed in the polymer and are used as face shield.These mosses can be encapsulated in the film and be used for food preservation (preservation) product as absorbing the packaging material and the lining of the gentle flavor of liquid.These mosses can be used for the cleaning of water treatment product with water among maintenance holding vessel, fishpond, swimming pool, rotary bathtub, the spa etc., and are used for water filtering device.These mosses can be used for black water and handle.These mosses can be made into the shape of (for example) disk or agglomerate, and be used for absorbing the water of cereal and other food.These mosses also can be used for fermentation control (as liquid or graininess).These mosses can be used for controlling fungi or the microbial diseases in lawn and the garden.These mosses can be used for (as) mould control product in hold-up vessel or the pipe-line system.
The invention provides a kind of method that suppresses growth of microorganism, described method comprises the material of the microorganism that is easy to grow is contacted with the mosses of rotting of the amount that effectively suppresses growth of microorganism that wherein said mosses are selected from wart bog moss, pappus bog moss and composition thereof.Described mosses can be compressed, and can be bar shapeds.Described mosses can be sterilized or sterilize with ethylene oxide treatment by the sterilization of HCS method, chemical treatment.Described mosses can be through acid solution, especially acetic acid solution washing.Described mosses can be washed with salting liquid after the acid solution washing.
The material of microorganism of being easy to grow can be a water.Water can be the water in spa, pool or the fishpond.The material of microorganism of being easy to grow can be a part such as skin, superficial cut, inner coelom or the internal injury position of human body.The material of microorganism of being easy to grow can be a cereal.
The invention provides a kind of method that suppresses growth of microorganism, described method comprise will effectively suppress the mosses of rotting of amount of growth of microorganism be placed in the load bearing component and the material of described load bearing component with the microorganism that is easy to grow contacted, wherein said mosses are selected from wart bog moss, pappus bog moss and composition thereof.Described load bearing component can be device, polymeric matrix, biological substrate or one or more layers film of implant into body.
The invention provides the method that preparation is used to suppress the mosses of growth of microorganism, described method comprises: (i) make unputrefied mosses drying; (ii) described mosses are sterilized.This method also can comprise the described mosses of compression, compresses described mosses and described mosses are cut into inch strips, and by HCS method, chemical treatment or ethylene oxide treatment described mosses is sterilized.Described mosses can be the peat mosses.Described mosses can be selected from wart bog moss, pappus bog moss and composition thereof.
The invention provides the method that a kind of preparation is used for the mosses of bacteria growing inhibiting, described method comprises: the mosses of (i) will not rotting contact with acid solution; (ii) make described mosses drying.This method contacts unputrefied mosses after can being included in step (i) with salting liquid.In one embodiment, described acid solution is an acetic acid solution.Described mosses can be the peat mosses.Described mosses can be selected from wart bog moss, pappus bog moss and composition thereof.
The invention provides a kind of kit that comprises the do not rot mosses and the load bearing component of sterilization.Described mosses can be compressed, and can be bar shapeds.
The invention provides a kind of method that suppresses growth of microorganism, described method comprises the water of the microorganism that is easy to grow is contacted with the mosses of rotting of the amount that effectively suppresses growth of microorganism, with regularly impact water with suitable chemical substance, wherein said mosses are selected from wart bog moss, pappus bog moss and composition thereof.Described chemical substance can be a potassium hydrogen persulfate.
The invention provides a kind of method for treating water, described method comprise with water with effectively remove in the water that the rotten mosses of cationic amount contact except that hydrogen ion, wherein said mosses are selected from wart bog moss, pappus bog moss and composition thereof.Described cation can be calcium or iron ion, can remove all calcium or iron ion in the water basically.Described mosses can be compressed, and can be bar shapeds.Described mosses can be sterilized or sterilize with ethylene oxide treatment by the sterilization of HCS method, chemical treatment.Described mosses can be through acid solution, especially acetic acid solution washing.These mosses can be washed with salting liquid after the acid solution washing.Water can be the water in spa, pool or the fishpond.
The invention provides a kind of method for treating water, described method comprises and is placed in the load bearing component with described load bearing component and contacts with water effectively removing in the water except that hydrogen ion the mosses of rotting of cationic amount that wherein said mosses are selected from wart bog moss, pappus bog moss and composition thereof.
The invention provides the device that uses in water, this device comprises: (i) have the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses.Described load bearing component can comprise float.Described load bearing component can comprise the column part under float and the float, and described column part has inner chamber and one or more permission flows into and the opening of outflow inner chamber.Described mosses can be packed in the mesh bag.Described load bearing component can comprise one or more counterweights.
Described mosses can be unputrefied mosses.Described mosses can be the peat mosses.Described mosses can be selected from wart bog moss, pappus bog moss and composition thereof.Described mosses can be compressed, and can be bar shapeds.Described mosses can be sterilized or sterilize with ethylene oxide treatment by the sterilization of HCS method, chemical treatment.Described mosses can be through acid solution, especially acetic acid solution washing.Described mosses can be washed with salting liquid after the acid solution washing.
The invention provides a kind of method that suppresses growth of microorganism, described method comprises the water of the device that suppresses growth of microorganism in the water being put into the microorganism that is easy to grow, and described device comprises: (i) have the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises the mosses of the amount of growth of microorganism in effective inhibition water.Water can be the water in spa, pool or the fishpond.Water can pump via described device.
The invention provides the kit of the device that does not rot mosses and in water, use that comprises sterilization, described device comprises the load bearing component that has inner chamber and one or more permission inflow and flow out the opening of inner chamber, and wherein said inner chamber can be sealed the residence fully and state mosses.Described kit can comprise one or more pH calibration tapes and/or potassium hydrogen persulfate.
The invention provides a kind of method that suppresses growth of microorganism, described method comprises the water of the device that suppresses growth of microorganism in the water being put into the microorganism that is easy to grow, with regularly impact water with suitable chemical substance, described device comprises: (i) have the load bearing component that inner chamber and one or more permission flow into and flow out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises the mosses of the amount of growth of microorganism in effective inhibition water.Described chemical substance can be a potassium hydrogen persulfate.
The invention provides a kind of method for treating water, described method comprises puts into water with a device, and described device comprises: (i) have the load bearing component that inner chamber and one or more permission flowed into and flowed out the opening of inner chamber; Contained mosses in the (ii) described inner chamber, wherein said load bearing component is sealed the residence fully and is stated mosses, and wherein said device comprises in effective removal water the described mosses of cationic amount except that hydrogen ion.Described cation can be calcium or iron ion, can remove all calcium or iron ion in the water basically.Described mosses can be compressed, and can be bar shapeds.Described mosses can be sterilized or sterilize with ethylene oxide treatment by the sterilization of HCS method, chemical treatment.Described mosses can be through acid solution, especially acetic acid solution washing.These mosses can be washed with salting liquid after the acid solution washing.Water can be the water in spa, pool or the fishpond.
Figure 1A-1C has illustrated appropriate device of the present invention.Figure 1A has shown the device 10 that floats in the water, and Figure 1B and 1C have shown end view and sectional view respectively.Adjusting device 10 is with the part of the peat mosses 15 of the compression of accepting to be cut into required size.Dotted line among Figure 1A and the 1B (phantom) expression mosses.The size that makes things convenient for that is used for the mosses of device 10 is about 6 * 1/4 * 1/4 inch (15.2 * 0.63 * 0.63cm).Heavily about 5 grams of mosses of this size.With mosses 15 nylon wire 16 of packing into, the size of nylon wire should allow to compress mosses and expand.Size of mesh opening should be able to keep little mosses particle and prevent that it from breaking apart and float.
Flowing through part 30 is cylinders of two parts elongation, and it has the center 32 of core or hollow.First 33 is connected in floating part 20, in the first 33 screw thread is arranged, and second portion 35 is attached on this screw thread.Can change the length that flows through part in this way.By the fixing position of second portion 35 of adjustable collar 34.Second portion 35 also is equipped with detachable cap 37, and its effect is to increase the weight of so that install 10 and float in the water according to mode shown in Figure 1.
Fig. 2 A explanation floats over the device 50 in the water.Device 50 comprises barrel portion 60, and it has the center 62 of core or hollow.Slit 64 and hole 66 allow water to enter the center of this hollow.As showing the most significantly among Fig. 2 B, with the mosses 55 that show with dotted line among net 52 encapsulation Fig. 2 A.Expand behind the mosses contact water, be full of the center 62 of hollow.The one end sealing of barrel portion 60, the other end is equipped with detachable cap 57.Cap 57 can increase the weight of, and keeps contacting with water so that install 60 maximum length.
Fig. 3 has shown the device 70 of the wall W that is connected in swimming pool, fishpond, hot tub etc.With net 72 encapsulation mosses 75, this net hangs on the carriage 77.The size of mesh opening of this net is enough to make the particle of mosses or fragment to be stayed in the net.Carriage is suspended on the wall, and this device can remain fixed on this position.Perhaps, device 70 can lie in pond or bathtub bottom.It can remain on the below attached to the there or by counterweight.The placement of also it can being connected with filter.
Embodiment
Embodiment 1
The northern wart bog moss of collecting from the Minnesota State, preparation is used for the bacteriostatic test.This mosses kind is confirmed through University of Minnesota, reaffirms after the acceptance.
All samples is placed in the plastic sack.All undressed mosses are stored in 4 ℃, up to processing by lab assistant.It is stand-by that all pre-dried outside mosses samples are stored in room temperature.Adopt following equipment:
A) agitator, the 1.25L capacity is (available from the Osterizer of Oster
)
B) distilled water is (available from Premium Water, Inc.)
C) tissue sieve, 1 glass of capacity is (available from the Cellector of E-C Apparatus Corp.
)
D) 1L glass beaker (commercially available Pyrex
)
E) aseptic polystyrene petri diss 100 * 15mm (available from Falcon)
F) aseptic polystyrene petri diss 150 * 15mm (available from VWR)
G) autoclaving pot (available from Market Forge)
H) the little spoon of metal experiment (16.5cm)/available from the Adison tissue forceps of VWR)
I) laminar flow hood (available from Baker)
Step:
1) from bag, takes out undressed mosses with hand, pick any visible, leaf and fragment, put into agitator.Be full of this agitator with mosses then, up to the mark line that approximately reaches 1L.
2) then 1L distilled water is added in the agitator, cover lid manually vibrated 30 seconds, and discharge water is to remove any residual dirt and fragment.This process repeats 2 times again with thorough washing mosses.
3) then, again agitator is filled distilled water, mixed mosses in each 30 seconds, distribute up to mosses homogeneous in whole sample with the pulse mode that is set at 4 and 5.
4) discharge water in the agitator then.Extrude all remaining water in the mosses by hand with the tissue sieve then.Then the mosses of extracting are placed in the clean 1L beaker.Repeating step 1-4 piles the mosses of processing in this 1L beaker.Then with this beaker with liquid setting (liquid setting) with 121 ℃ of 15psi (103.4MPa) autoclaving 20 minutes, it is at room temperature cooled off.
5) after the cooling, mosses are moved on in the laminar flow hood, be placed on carefully in the markd petri diss of weighing in advance with little spoon in aseptic experiment chamber and pliers.Need SC in order to avoid pollute these mosses and pack each ware with uniform way.After the packing, make this ware keep opening wide at least 72 hours, up to the mosses drying.Cover dry ware and be placed on laminar flow hood stand-by.
Embodiment 2
Available from Sutton ' s Moss, the pappus bog moss of Canada (collecting) at New Zealand, preparation is used for the bacteriostatic test.Confirmed this mosses kind after the acceptance.The processing of this mosses sample is identical with the foregoing description 1.Adopt following equipment:
A) asepsis injector, each self-capacity are 30cc or 60cc (available from Becton Dickenson)
B) asepsis injector filter, 0.45 μ m (micron) and 0.20 μ m (available from the Acrodisc of Pall Gellman)
C) Adison tissue forceps (available from VWR)
D) 50cc polypropylene scale test tube, aseptic packaging (available from Falcon)
E) cere (Parafilm
, available from American National Can)
F) laminar flow hood (available from Baker Company)
G) micropipettor of aseptic scale polypropylene tip is housed, 25mL (available from Becton Dickenson)
H) 10M HCl and 10M NaOH
I) pH meter (available from Beckman Omega 40)
Reagent and pharmaceutical solutions: (according to the liquid that is used to handle)
A) Bacto
TMTryptic soy broth, 30g/L (Becton Dickenson)/MEM-α (Gibco)
B) phosphate buffered saline (PBS) (1x), pH7.1 is (available from Gibco
TM)
C) hplc grade water (available from J.T.Baker)
Step
A) all liq that is used to handle is carried out autoclaving or filtration sterilization, be stored under the suitable temperature up to being used for this scheme.In laminar flow hood, finish all processing steps with sterile working.Face with preceding used tissue forceps is carried out autoclaving.
B) weigh after the dry mosses, treat liquid is sucked petri diss, concentration is the dry mosses of 1 milliliter of treat liquid/50mg.If adopt the concentration beyond the 50mg/mL, this method of corresponding change.
C) with cere (Parafilm
) seal the petri diss that mosses are housed, in 4 ℃ of refrigerators, placed 1 hour.
D) from refrigerator, take out petri diss, take out mosses and clamp pack into 30cc or 60cc syringe with sterile tissue with little spoon.Again insert the piston of this syringe, liquid is clamp-oned in the aseptic 50mL polypropylene test tube liquid that might squeeze out up to squeezing out.
E) press juice filters by 0.45 μ m syringe filter, then by 0.2 μ m syringe filter, be stored in 4 ℃ stand-by.
F) with the pH of 1.0M HCl/NaOH adjustment filtered sample,, be stored in 4 ℃ once more, measure up to being used for bacteriostatic with 0.2 μ m syringe filter filtration sterilization.
Embodiment 3
This experiment is measured bacterial growth amount in the tryptic soy broth (TSB) with inhibition test.Preparation is used for all the wart bog mosses and the pappus bog moss extract of this test as mentioned above.Also prepare TSB, autoclaving and be stored in 4 ℃ stand-by.Adopt following equipment:
A) Beckman
The DU-64 spectrophotometer
B) incubator (available from Boekel Instruments Model#133000)
C) 5mL and 5OmL polystyrene test tube (available from Falcon)
D) 10 μ L and 1mL polypropylene tip (available from Pipetman)
Reagent and solution:
A) Bacto
Tryptic soy broth (TSB), 30g/L (available from Becton Dickenson)
B) at grown at least Escherichia coli (Escherichia Coli) the freezing cultivation mother liquors (clinical isolates) of 3 exponential phases of TSB
C) grown at least staphylococcus aureus (Staphylococcus Aureus) of 3 exponential phases of TSB.(ATCC bacterial strain #29213 (American type culture collection, Manassas, VA))
D) distilled water (available from Premium Water Inc.)
E) the TSB extract of wart bog moss and New Zealand's mosses
Step:
1) passes through 30g/L Bacto
Tryptic soy broth adds preparation TSB nutrient broth in the distilled water.Stir this solution with stirring rod and dissolve, 121 ℃ of autoclavings 20 minutes up to all powder.The as above described preparation wart of B bog moss extract.The TSB sample that 1mL solution, TSB or mosses are handled sucks 3-5mL polystyrene test tube.
2) Escherichia coli that are stored in-20 ℃ and the freezing equal portions of staphylococcus aureus are thawed to room temperature.In case after thawing, each 100 μ L adds among the TSB of 10mL equal portions with the bacterium mother liquor.Cover each test tube then, fully mix, mark and put into 37 ℃ incubator.
3) 10 μ L bacterium mother liquors are sucked in the 1mL solution, this test tube is hatched required time at 37 ℃.Do not add bacterium in 1 test tube of each sample and time point, blank to be used as.
4) at the appointed time take out solution and put into ice bath o'clock from 37 ℃ of incubators.Then rapidly on spectrophotometer with 550nm to the sample reading.Absolute OD value is that sample subtracts blank.By measuring inhibition with respect to the reduction percentage of suitable TSB control sample OD value.
Embodiment 4
Following data declaration is handled the effect of bacterial growth media (being tryptic soy broth) with various mosses according to the step of embodiment 3.Non-bog moss kind is preventing that performance is very poor aspect the Escherichia coli growth.In the mosses of following mensuration, can prevent most effectively that the bog moss of Escherichia coli growth from being wart bog moss and pappus bog moss." N " refers to testing time, and " scope " is the highest and minimum that these tests obtain; " mean value " refers to the mean value tested.
Embodiment 5
Handle TSB with the wart bog moss, measure the ability of this solution support bacteria growth according to embodiment 3.Reported that the bacterial growth to Escherichia coli (clinical isolates) and staphylococcus aureus (ATCC bacterial strain #29213) suppresses percentage.Below report the TSB solution (MT-TSB) that TSB control sample and mosses are handled.The OD that the sample OD (measured value) that measures deducts blank (B) obtains reported values.
Embodiment 6
The present embodiment proof is not is not killed and wounded bacterium with the mosses processing but is suppressed its growth.Use Probes, Eugene, the L-7012 fluoremetry kit measurement bacterium vigor of Oregon available from Molecular.This system adopts green and the red fluorescence nucleic acid dye mixture with the separating capacity that penetrates bacterium alive and dead bacterial cell.Green fluorescence dyestuff (emission wavelength 500nm) can be incorporated into bacterium alive and dead bacterium.Red fluorescence dyestuff (emission wavelength 650nm) only is incorporated into dead bacterium.Therefore, the dyeing ratio that contains the bacteria samples of more dead bacterium can change.Data show, for Escherichia coli and staphylococcus aureus, the bacterium that lives keeps identical with control sample with the ratio of dead bacterium.
Step:
1. 100 μ L (preparation of the control TSB of pH and wart bog moss sample respectively as embodiment 1 and 2 as described in) 37 ℃ in the 10mL medium hatched 3 hours.
2. centrifugal this mixture is resuspended in bacterium 10mL phosphate buffered saline (PBS) (PBS) then to concentrate bacterium.
3. respectively that 3mL is resuspended bacterium adds in three cuvettes.
4. 40 μ L Styo-9 dyestuffs are mixed with 40 μ L iodate, the third ingot dyestuff.Should be careful during use, because these compounds are considered to carcinogenic substance.
5. the dye solution that 9 μ L are mixed adds each cuvette, in the dark stores 15 minutes.
6. prepare two and do not have the PBS cuvette of dyestuff and PBS cuvette dyestuff that two contain dyestuff as blank.
7. fully mix this solution.Measure fluorescence intensity at 500nm (absorbing) and 650nm (absorbing) at the 490nm place at the 480nm place.The ratio of these two values is relevant with the vigor degree of bacterial cultures.
Following table has been reported the fluorescence intensity of bacteria samples under two wavelength in two kinds of different medium.The fluorescence intensity of 500nm is relevant with the vigor degree of bacterial cultures divided by the ratio of the fluorescence intensity generation of 650nm.Reported the mean value of the TSB sample (MT-TSB) of three kinds of TSB and three kinds of mosses-processing below.Under each situation, will suppress percentage and compare with control sample.
The vitality test of Escherichia coli (clinical isolates)
The ratio of medium 500nm 650nm 500/650
TSB 505.117 5.783 87.3228
The MT-TSB 130.1833 1.5167 85.8352 of wart bog moss
The vitality test of staphylococcus aureus (ATCC bacterial strain #29213)
The ratio of medium 500nm 650nm 500/650
TSB 80.117 3.533 22.6745
The MT-TSB 45.055 2.0667 21.7984 of wart bog moss
Data show, TSB does not have significance to change with the ratio of the TSB that mosses are handled, and the effect that mosses are described is antibacterial but not sterilization.
Embodiment 7
Handle various bacteriums according to embodiment 3 usefulness wart bog moss TSB (concentration 50mg/mL).Reported that bacterial growth suppresses percentage.Below report the TSB solution (MT-TSB) that TSB control sample and mosses are handled.Deduct optical density (OD) acquisition " value " of blank (B) by the mensuration OD (measured value) of sample.
Carried out twice research, hereinafter referred to as (1) and (2).
The embodiment 8--effect of acid treatment mosses
With the compressed rod of pappus bog moss (available from Sutton ' s Moss, Canada (collecting)) immerses four kinds of distilled water solution (Fe standard items in the concentrated hydrochloric acid that Fe concentration increases gradually in New Zealand, 0,0.5,5,50mg/L, available from RiccaChemicals, Arlington, Texas) in, making mosses concentration is 50mg/ml.The mosses that to soak into are 4 ℃ of store overnight.With the syringe Fe solution of squeezing out, filter, with inducing the plasma atomic emission spectrum determination method assay determination Fe of coupling.The result shows that mosses combine the Fe of significant quantity, in the 50mg/L sample up to 15mg/L.Undertaken this experiment by the distilled water wash mosses, obtain analog result.Yet, notice that (spiked) sample pH value of Fe reinforcement is low.Owing to realize optimum combination when pH 4-6, we heighten 4-7 with pH in the next round test.When the pH of Fe sample raise, Fe began precipitation.Mosses have been removed all Fe (up to 25mg/L) in the sample.Determine that then the ion that is incorporated into mosses before usefulness can influence cation in conjunction with test, should study the cationic method of removing in the mosses.
In the method for exploitation acid elution peat mosses (be not peat moss, peat moss is the mosses of rotting), two kinds of different acid have at first been used.The a collection of mosses of continuous stirring (about 5g) are used dense HNO in 3.5L distilled water
3PH is adjusted into 1.For the mosses of other batch, adopt 2% glacial acetic acid solution.Agitator treating thing 1 hour filters supernatant then, and new wash solution is added mosses.Repeat acid elution altogether four times.Behind the acid elution, use distilled water wash in the same manner 4 times.When distilled water wash finished, its electrical conductivity was similar to distilled water.The ionic weight of these two kinds acid removals is similar; Therefore generally washed mosses afterwards with acetate.
Determined the raising that under neutral pH soluble metal is more suitable for measuring binding capacity and mensuration and combines with acid elution peat mosses (with respect to do not wash combining of mosses).Select calcium to be used for this purpose.Used mosses are present embodiment described pappus bog mosses before, and used acid is 2% glacial acetic acid solution, washs as described in the previous paragraph.First test shows and estimates on the contrary, does not wash the maximum of mosses combination, the mosses combination of acid elution minimum.When sample for reference pH, find that binding ability is relevant with pH.The mosses of acid elution have reduced the pH of solution, and this can influence ions binding.For eliminating this problem, the mosses of using the washing of high level salt solution washing acid are to replace the H+ ion with Na.High level salt solution is the 1M sodium acetate, continues stir about 5g mosses 1 hour, repeated washing then in this kind of 3.5L sodium acetate solution.After this twice sodium acetate solution washing, use distilled water wash 4 times, washed 1 hour at every turn, each washing is carried out in the same manner.Measure the mosses of acid/salt washing; The pH of press juice does not reduce, and the combination that demonstrates than distilled water wash and unwashed mosses significantly improves (Ca concentration is represented with ppm) as shown in the table.
Therefore the variation of extract pH still is difficult to distinguish the effect of acid elution and pH up to 2 pH units.Therefore developed the new method of measuring the mosses combination, it can still adjust pH under the situation in sample solution in mosses.Finish the method with porous plate, little stirring rod and 10mg/ml mosses concentration.Solution is on the mosses approximately after half an hour, the pH of all solution adjusted in 0.25 pH unit of 6.5.The mosses that this can measure the mosses of acid/salt washing and only use acid elution.The result is as follows.Shown in mean value be the mean value of two samples.
Because pH adjusts to mutual difference in 0.5 pH unit; Eliminated the pH effect, the binding capacity that can be observed the mosses of acid elution significantly improves.The combination of the mosses of acid/salt washing is better slightly than the mosses of only using acid elution, distilled water wash better slightly than unwashed mosses, but be enough to hardly be called significantly.
Therefore, prove that mosses can be in conjunction with Fe and Ca ion, therefore can be effective to water treatment, because one or both that remove these two kinds of ions are purposes of water treatment.
The purpose that above-mentioned specification and accompanying drawing are provided is to describe embodiment of the present invention, and is not intended to limit by any way the scope of the invention.It will be understood by those skilled in the art that and to carry out various modifications and change, and do not deviate from the spirit or scope of the present invention.Therefore, the present invention is intended to cover modification of the present invention and change, if they enter the scope of the claims and the equivalent form of value thereof.
Claims (21)
1. method that suppresses growth of microorganism, described method comprises the water of the microorganism that is easy to grow is contacted with the mosses of rotting of the amount that effectively suppresses growth of microorganism, wherein said mosses are selected from wart bog moss, pappus bog moss and composition thereof, described method comprises puts into load bearing component with described unputrefied mosses earlier, then described load bearing component is contacted with water, described load bearing component has inner chamber and one or more permission flows into and the opening of outflow inner chamber, described mosses are included in the described inner chamber, and described load bearing component is sealed the residence fully and stated mosses.
2. the method for claim 1 is characterized in that, described mosses are wart bog mosses.
3. the method for claim 1 is characterized in that, described mosses are pappus bog mosses.
4. the method for claim 1 is characterized in that, described mosses only comprise the mixture of wart bog moss and pappus bog moss.
5. each described method in the claim as described above is characterized in that described mosses are compressed.
6. method as claimed in claim 5 is characterized in that described mosses are bar shapeds.
7. as each described method in claim 1 to 4 and the claim 6, it is characterized in that described mosses are through sterilization processing.
8. method as claimed in claim 5 is characterized in that described mosses are through sterilization processing.
9. as each described method in claim 1 to 4 and the claim 6, it is characterized in that described mosses are washed through acid solution.
10. method as claimed in claim 5 is characterized in that described mosses are washed through acid solution.
11. method as claimed in claim 9 is characterized in that, described mosses are washed through acetic acid solution.
12. method as claimed in claim 10 is characterized in that, described mosses are washed through acetic acid solution.
13. method as claimed in claim 9 is characterized in that, described mosses are washed with salting liquid after the acid solution washing.
14. method as claimed in claim 10 is characterized in that, described mosses are washed with salting liquid after the acid solution washing.
15. the method for claim 1 is characterized in that, described water is the water in the hot tub, water in the pool or the water in the fishpond.
16. the method for claim 1 is characterized in that, described water pumps via described load bearing component.
17. method as claimed in claim 16 is characterized in that, described load bearing component is a polymeric matrix, biological substrate or one or more layers film.
18. the method for claim 1 is characterized in that, described load bearing component comprises float.
19. the method for claim 1 is characterized in that, described load bearing component comprises the column part under float and the float, and described column part has inner chamber and one or more permission flows into and the opening of outflow inner chamber.
20. the method for claim 1 is characterized in that, described mosses are contained in the mesh bag.
21. the method for claim 1 is characterized in that, described load bearing component comprises one or more counterweights.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US56208904P | 2004-04-14 | 2004-04-14 | |
| US56219604P | 2004-04-14 | 2004-04-14 | |
| US60/562,089 | 2004-04-14 | ||
| US60/562,196 | 2004-04-14 | ||
| PCT/US2005/012915 WO2005102935A2 (en) | 2004-04-14 | 2005-04-14 | Methods of inhibiting microorganism growth using moss and devices for water treatment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1942405A CN1942405A (en) | 2007-04-04 |
| CN1942405B true CN1942405B (en) | 2011-05-11 |
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ID=34966785
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|---|---|---|---|
| CN2005800115381A Expired - Fee Related CN1942405B (en) | 2004-04-14 | 2005-04-14 | Methods of inhibiting microorganism growth using moss and devices for water treatments |
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| Country | Link |
|---|---|
| EP (1) | EP1747174A2 (en) |
| JP (1) | JP2007533378A (en) |
| CN (1) | CN1942405B (en) |
| AU (1) | AU2005235588B2 (en) |
| HK (1) | HK1104810A1 (en) |
| NO (1) | NO20065204L (en) |
| NZ (1) | NZ551183A (en) |
| WO (1) | WO2005102935A2 (en) |
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| CN103209592A (en) * | 2010-08-30 | 2013-07-17 | 安伯洛股份有限公司 | Inhibition of biofilm formation and removal of biofilm by use of moss |
| US9005449B2 (en) | 2011-09-07 | 2015-04-14 | Embro Corporation | Use of moss to reduce disinfection by-products in water treated with disinfectants |
| AU2012328894A1 (en) * | 2011-10-24 | 2014-04-10 | Embro Corporation | Removal and inhibition of scale and inhibition of corrosion by use of moss |
| US9795809B2 (en) | 2013-12-23 | 2017-10-24 | Embro Corporation | Use of moss to improve dental health |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85103723A (en) * | 1984-07-16 | 1986-12-03 | 江森及江森有限公司 | Antimicrobial peat moss composition |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4002566A (en) * | 1975-04-07 | 1977-01-11 | Smith John D | Water purifying systems |
| GB2134793B (en) * | 1983-02-11 | 1987-02-25 | Vernon Carus Ltd | Absorbent media comprising sphagnum moss |
| US4618496A (en) * | 1984-07-16 | 1986-10-21 | Johnson & Johnson | Antimicrobial peat moss composition |
| NO304148B1 (en) * | 1995-08-25 | 1998-11-02 | Oyvind Bones | Sorbent for the sorption of oil and its use |
| CA2188198C (en) * | 1995-10-19 | 2004-04-06 | Stephen G. Simmering | Peat bale filtration element |
| US6620321B2 (en) * | 1999-12-30 | 2003-09-16 | Edward R. Festa | Biofilter system and method for treating wastewater |
| JPWO2005081641A1 (en) * | 2004-03-01 | 2007-10-25 | 志村 光春 | Water purification method and purification system |
-
2005
- 2005-04-14 AU AU2005235588A patent/AU2005235588B2/en not_active Ceased
- 2005-04-14 JP JP2007508581A patent/JP2007533378A/en active Pending
- 2005-04-14 EP EP05738766A patent/EP1747174A2/en not_active Withdrawn
- 2005-04-14 CN CN2005800115381A patent/CN1942405B/en not_active Expired - Fee Related
- 2005-04-14 WO PCT/US2005/012915 patent/WO2005102935A2/en active Application Filing
- 2005-04-14 NZ NZ551183A patent/NZ551183A/en not_active IP Right Cessation
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- 2006-11-13 NO NO20065204A patent/NO20065204L/en not_active Application Discontinuation
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85103723A (en) * | 1984-07-16 | 1986-12-03 | 江森及江森有限公司 | Antimicrobial peat moss composition |
Non-Patent Citations (1)
| Title |
|---|
| 无.科学家发现泥炭可用于鱼类保鲜.四川兽牧兽医.2001,28(9),56. * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005102935A3 (en) | 2005-12-29 |
| EP1747174A2 (en) | 2007-01-31 |
| NZ551183A (en) | 2009-09-25 |
| JP2007533378A (en) | 2007-11-22 |
| AU2005235588A1 (en) | 2005-11-03 |
| NO20065204L (en) | 2006-11-13 |
| HK1104810A1 (en) | 2008-01-25 |
| WO2005102935A2 (en) | 2005-11-03 |
| CN1942405A (en) | 2007-04-04 |
| AU2005235588B2 (en) | 2010-12-23 |
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