CN1939427B - Prickly polygonum extract, its preparation, medicinal composition and use - Google Patents
Prickly polygonum extract, its preparation, medicinal composition and use Download PDFInfo
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Abstract
An extract of Herba polygoni perfoliati for preparing the composite medicines used to treat non-specific vulvitis, vulva ulcer, vulva itching, vagnitis, cervicitis, pelvice inflammation, amenorrhoea, menalgia, bone fracture, injury, etc is prepared from the fresh Herba polygoni perfoliati without root through drying in the air, breaking, extracting in organic solvent or water 2-4 times, collecting liquid extracting, concentrating and drying.
Description
Technical field: the present invention relates to a kind of Herba Polygoni cymosi extract and preparation method thereof, pharmaceutical composition and purposes, belong to technical field of pharmaceuticals.
Background technology: Herba Polygoni cymosi is the dry aerial parts of polygonaceae plant Herba Polygoni cymosi, is the peculiar medicine commonly used in southwest, has long medicinal history.According to Miao ethnic group's medication experience among the people, its aerial parts is pharmaceutically acceptable, has heat-clearing and toxic substances removing, and is inducing diuresis and reducing edema, functions such as dissipating blood stasis hemostasis.Herba Polygoni cymosi both can singly be used, and also can go into compound recipe.Among the people in Miao ethnic group, compatibilities such as Herba Polygoni cymosi and Radix Sophorae Flavescentis, Cortex Phellodendri, Caulis Spatholobi, Herba Leonuri have the function of heat clearing and damp drying, blood circulation promoting and blood stasis dispelling, adjusting meridian and stopping leukorrhea, are used for the treatment of cervicitis, vaginitis, menoxenia, red leucorrhea, dysmenorrhea, adnexitis.
At present, what the extraction of Herba Polygoni cymosi was generally adopted is conventional decoction and alcohol sedimentation technique or alcohol extracting method, but does not appear in the newspapers as yet for the pharmaceutical research of Herba Polygoni cymosi extract.The applicant is through discovering extensively and profoundly, have antibiotic significantly, antiinflammatory, analgesic, analgesic activity by Herba Polygoni cymosi is extracted the Herba Polygoni cymosi extract that obtains under certain condition with organic solvent, water-containing organic solvent or water, and demonstrate the effect of good curing gynaecopathia and traumatology disease.
Summary of the invention:
The objective of the invention is to: a kind of Herba Polygoni cymosi extract and preparation method thereof, pharmaceutical composition and purposes are provided.That Herba Polygoni cymosi extract of the present invention has is antibiotic significantly, antiinflammatory, analgesic, analgesic activity, particularly non-specific vulvitis, vulval ulcer, pruritus vulvae, vaginitis, cervicitis, pelvic inflammatory disease, amenorrhea, dysmenorrhea, injury from falling down is had therapeutical effect.
The present invention is achieved in that the preparation method of Herba Polygoni cymosi extract is: with the Herba Polygoni cymosi aerial parts dried and crushed of fresh collection, obtain the Herba Polygoni cymosi coarse powder, extract 2～4 times with organic solvent, water-containing organic solvent or water reflux or diafiltration then, obtain Herba Polygoni cymosi extract behind the extracting solution concentrate drying.
Described organic solvent is methanol, ethanol, propanol, butanols, acetone, chloroform, dichloromethane, methyl acetate, ethyl acetate, ether, petroleum ether or gasoline.
Specifically, the preparation method of Herba Polygoni cymosi water extract is: it is an amount of to get Herba Polygoni cymosi, is ground into coarse powder, add 10 times of water gagings and decoct 2 times, 2 hours for the first time, 1.5 hours for the second time, filter, merging filtrate, being concentrated into 60 ℃ of survey relative densities is 1.30, put cold, add ethanol and make that to contain the alcohol amount be 70%, standing over night, getting supernatant, to be evaporated to relative density be 1.25 thick paste, 75 ℃ of drying under reduced pressure, promptly.
The preparation method of Herba Polygoni cymosi alcohol extract is: it is an amount of to get Herba Polygoni cymosi, is ground into coarse powder, adds 8 times of amount alcohol reflux of 75% 2 times, each 2 hours, filter merging filtrate, decompression recycling ethanol, it is 1.25 thick paste that surplus liquid is concentrated into relative density, drying under reduced pressure, promptly.
Extract the Herba Polygoni cymosi extract that obtains by above-mentioned Herba Polygoni cymosi extract preparation method.
The pharmaceutical composition of forming by above-mentioned Herba Polygoni cymosi extract: comprise Herba Polygoni cymosi extract and one or more pharmaceutical carriers or excipient as active component.
Herba Polygoni cymosi extract of the present invention is used to prepare the medicine for the treatment of gynaecopathia.
Described gynaecopathia is non-specific vulvitis, vulval ulcer, pruritus vulvae, vaginitis, cervicitis, pelvic inflammatory disease, amenorrhea or dysmenorrhea.
Herba Polygoni cymosi extract of the present invention is used to prepare the medicine for the treatment of traumatology disease.
Described traumatology disease is an injury from falling down.
Herba Polygoni cymosi extract of the present invention can be separately or is used with pharmaceutical compositions, therefore also relates to containing as the Herba Polygoni cymosi extract of the effective dose of active ingredient and the pharmaceutical composition of conventional medicine excipient or adjuvant.Usually pharmaceutical composition of the present invention contains the Herba Polygoni cymosi extract of 0.1-90% weight.Pharmaceutical composition can prepare according to methods known in the art.If desired, Herba Polygoni cymosi extract and one or more solids or liquid medicine excipient and/or adjuvant can be combined, make the suitable administration form or the dosage form that can be used as human.
Herba Polygoni cymosi extract of the present invention or contain its pharmaceutical composition can the unit dosage form administration, route of administration can be intestinal or non-intestinal, as oral, muscle, subcutaneous, nasal cavity, oral mucosa, skin, peritoneum or rectum etc.Form of administration is tablet, capsule, drop pill, aerosol, pill, powder, solution, suspensoid, Emulsion, granule, liposome, transdermal agent, buccal tablet, inspection agent, unguentum, patch, injection, lyophilized injectable powder etc. for example.Can be ordinary preparation, slow releasing preparation, controlled release preparation and various particulate delivery system.For the unit form of administration is made tablet, can be extensive use of various carrier well known in the art: for example diluent and absorbent, as starch, dextrin, calcium sulfate, lactose, mannitol, sucrose, sodium chloride, glucose, carbamide, calcium carbonate, kaolin, microcrystalline Cellulose, aluminium silicate etc.; Wetting agent and binding agent are as water, glycerol, Polyethylene Glycol, ethanol, propanol, starch slurry, dextrin, syrup, Mel, glucose solution, mucialga of arabic gummy, gelatine size, sodium carboxymethyl cellulose, lac, methylcellulose, potassium phosphate, polyvinylpyrrolidone etc.; Disintegrating agent is as dry starch, alginate, agar powder, laminaran, sodium bicarbonate and citric acid, calcium carbonate, polyoxyethylene sorbitol fatty acid ester, dodecyl sodium sulfate, methylcellulose, ethyl cellulose etc.; The disintegrate inhibitor is as sucrose, glyceryl tristearate, cocoa butter, hydrogenation wet goods; Absorption enhancer is as quaternary ammonium salt, sodium lauryl sulphate etc.; Lubricant is as Pulvis Talci, silicon dioxide, corn starch, stearate, boric acid, liquid paraffin, Polyethylene Glycol etc.Tablet further can also be made coated tablet, for example sugar coated tablet, thin membrane coated tablet, ECT, or double-layer tablet, multilayer tablet.For pill is made in the administration unit, can be extensive use of various carrier well known in the art: for example diluent and absorbent, as glucose, lactose, starch, cocoa butter, nitrogenize vegetable oil, polyvinylpyrrolidone, Kaolin, Pulvis Talci etc.; Binding agent such as arabic gum, Tragacanth, gelatin, ethanol, Mel, liquid sugar, rice paste or batter etc.; Disintegrating agent is as agar powder, dry starch, alginate, dodecyl sodium sulfate, methylcellulose, ethyl cellulose etc.For the inspection agent is made in the administration unit, can be extensive use of various carrier well known in the art: for example ester of Polyethylene Glycol, lecithin, cocoa butter, higher alcohol, higher alcohol, gelatin, semi-synthetic glyceride etc.For capsule is made in the administration unit, the effective ingredient Herba Polygoni cymosi extract is mixed with above-mentioned various carriers, and the mixture that will obtain thus places hard ' Yanming ' capsules for clearing or soft capsule.Also the effective ingredient Herba Polygoni cymosi extract can be made microcapsule, be suspended in and form suspensoid in the aqueous medium, in the hard capsule of also can packing into or make injection and use.For injection preparation is made in the administration unit, as solution, Emulsion, lyophilized injectable powder and suspensoid, can use this area all diluent commonly used, for example water, ethanol, Polyethylene Glycol, 1, the isooctadecanol of ammediol, ethoxylation, the isooctadecanol of polyoxyization, Polyoxyethylene Sorbitol Fatty Acid Esters etc.In addition, ooze injection, can in injection preparation, add proper amount of sodium chloride, glucose or glycerol, in addition, can also add conventional cosolvent, buffer agent, pH regulator agent etc. in order to prepare etc.
In addition, as needs, also can in pharmaceutical preparation, add coloring agent, antiseptic, spice, correctives, sweeting agent or other material.
The dosage of Herba Polygoni cymosi extract of the present invention depends on many factors, for example to prevent or treat the character and the order of severity of disease, the sex of patient or animal, age, body weight and individual reaction, used particular compound, route of administration and administration number of times etc.Above-mentioned dosage can the single dose form or be divided into several, for example two, three or four dosage form administrations.
In order to verify the reasonability of preparation method of the present invention, the applicant has carried out experimental study and screening to the extraction process of Herba Polygoni cymosi, and is specific as follows:
One, the research of Herba Polygoni cymosi water extraction process
The principal element that influence decocts has: pulverizing medicinal materials granularity, decoction number of times, amount of water and decocting time etc., the applicant is through discovering: the extraction process that optimizes by the following method can be thought the best water extraction process of Herba Polygoni cymosi, and the extract curative effect of gained is best.
It is an amount of to get the Herba Polygoni cymosi medical material, and according to the form below 1 test is carried out the preferred of extraction process with the pharmacologically active scoring of paste-forming rate and Herba Polygoni cymosi as evaluation index, the results are shown in Table 2, table 3:
The factor level table of table 1 Herba Polygoni cymosi Study on extraction
Table 2 Herba Polygoni cymosi extraction process preferred result table
Table 3 orthogonal test analysis of variance table
| Soruces of variation | SS | Degree of freedom | It is mean square to make a variation | Statistic F | Significance |
| A | 2.1425 | 2 | 1.0712 | 5.1952 | |
| B | 13.7421 | 2 | 6.8710 | 33.3223 | ?P＜0.05 |
| C | 14.1274 | 2 | 7.0637 | 34.2565 | ?P＜0.05 |
| D (error) | 0.4012 | 2 | 0.2006 | 1 | |
| Amount to | 30.4132 | 8 |
Annotate: F
0.05(2,2)=19.00
Result of study shows: each factor influences size sequence to the Herba Polygoni cymosi water extraction process and is: C＞B＞A＞D, and wherein two factors of B, C have significant difference, B2＞B3＞B1, C2＞C3＞C1, therefore, extraction time selects 2 times, and amount of water selects 10 times; Two factors of A, D do not have significant difference, but consider and produce reality, select coarse powder, and with the convenient extracting solution that filters, decocting time is decided to be 2 hours for the first time, is decided to be 1.5 hours for the second time.
Therefore, the optimum extraction process of Herba Polygoni cymosi water extract is: it is an amount of to get Herba Polygoni cymosi, is ground into coarse powder, and the decocting that adds 10 times of amounts boils 2 times, 2 hours for the first time, 1.5 hours for the second time, filters merging filtrate.
The Herba Polygoni cymosi medical material is after the water concentration contracts, and remove impurity is quite crucial, and the applicant adopts Different concentrations of alcohol to carry out precipitate with ethanol to remove impurity, and method is as follows:
Get the Herba Polygoni cymosi coarse powder, carry decocting condition by preferred water and decoct extraction, (60-75 ℃ of concentrating under reduced pressure,-0.08Mpa) to relative phase density 1.30 (60 ℃), shake up, measure a certain amount of concentrated solution, add ethanol, making ethanol content is 60%, 70%, 80%, and precipitate with ethanol 24 hours is got the supernatant decompression recycling ethanol, volatilize, drying under reduced pressure (65-80 ℃ ,-0.08Mpa), claim ethanol extract weight; Alcohol hypostasis drying under reduced pressure (65-80 ℃ ,-0.08Mpa), claim impurity weight.Measure the pharmacologically active of ethanol extract, result of the test sees Table 4.
Table 4 impurity removal process result of study table
| Sequence number | Amount of liquid medicine (ml) | Contain alcohol amount (%) | Ethanol extract amount (g) | Alcohol hypostasis amount (g) | The pharmacologically active scoring |
| 1 | 125 | 60 | ?11.3658 | ?7.5442 | 90 |
| 2 | 125 | 80 | ?10.5629 | ?8.2025 | 89 |
| 3 | 125 | 70 | ?10.4425 | ?8.8284 | 92 |
| 4 | 125 | 0 | ?19.3653 | ?0 | 86 |
Result of study shows: alcohol precipitation concentration is 70% o'clock, and the impurity of removing is maximum, and simultaneously pharmacologically active is the highest, therefore, selects for use 70% concentration of alcohol as the concentration of removing impurity.
The research of concentration technology: the applicant has investigated concentrating under reduced pressure and normal pressure concentrates two kinds of methods, find through preferred, concentrating under reduced pressure and normal pressure concentrate two kinds of methods relatively, concentrating under reduced pressure is concentrated fast than normal pressure, save time, and pharmacologically active is concentrated higher than normal pressure, therefore, selects the method for concentration of concentrating under reduced pressure as the preparation of this extract.
Drying process: the applicant discovers that baking temperature has certain influence to the pharmacologically active of extract, and this extract is best at the pharmacologically active of 75 ℃ of drying under reduced pressure, therefore, selects the drying means of 75 ℃ of drying under reduced pressure as this extract.
Two, the research of Herba Polygoni cymosi alcohol extraction technology
The principal element that influence is extracted has: extraction time, solvent consumption, solvent concentration and extraction time etc., the applicant is through discovering: the extraction process that optimizes by the following method can be thought the best alcohol extraction technology of Herba Polygoni cymosi, and the extract curative effect of gained is best.
It is an amount of to get the Herba Polygoni cymosi medical material, is ground into fine powder, tests by table 5, the results are shown in Table 6, table 7:
The factor level table of table 5 Herba Polygoni cymosi alcohol extraction technical study
Table 6 Herba Polygoni cymosi alcohol extraction optimal process is table as a result
Table 7 orthogonal test analysis of variance table
| Soruces of variation | SS | Degree of freedom | It is mean square to make a variation | Statistic F | Significance |
| A | 2.1249 | 2 | 1.0625 | 4.6681 | |
| B | 12.2741 | 2 | 6.1371 | 26.9642 | ?P＜0.05 |
| C | 14.1238 | 2 | 7.0619 | 31.0277 | ?P＜0.05 |
| D (error) | 0.4552 | 2 | 0.2276 | 1 | |
| Amount to | 28.9780 | 8 |
Annotate: F
0.05(2,2)=19.00
Result of study shows: each factor to Herba Polygoni cymosi alcohol extraction technogenic influence size order is: C＞B＞A＞D, by intuitive analysis, select C2, B2, A2, D2 as optimum extraction process, that is: Herba Polygoni cymosi is an amount of, be ground into coarse powder, add 8 times of amount alcohol reflux of 75% 2 times, each 2 hours, filter, it is 1.25 thick paste that merging filtrate, decompression recycling ethanol, surplus liquid are concentrated into relative density, drying under reduced pressure promptly gets the Herba Polygoni cymosi alcohol extract.
In order to ensure effect of the present invention, the applicant has carried out biological activity test to described Herba Polygoni cymosi extract, and is specific as follows:
Observation index: Ovum Gallus domesticus album causes rat pedal swelling experiment (antiinflammatory action); Mice caused by dimethylbenzene xylene auricle swelling degree test (antiinflammatory action); White mice is turned round body experiment (analgesic activity); Yeast pyrogenicity test (refrigeration function).
The Herba Polygoni cymosi extract that this biological activity test adopted is with ethanol as extracting the extract that solvent obtains.
1. Herba Polygoni cymosi extract causes the influence of pedal swelling behind the rat to Ovum Gallus domesticus album:
Method and result: get 30 of Wistar rat, weigh, use the picric acid labelling, be divided into 3 groups, rat right hind leg is fixed with hands, make a sign with ball pen in its ankle, the right hind of animal immerses in the sufficient capacity measuring device, and immersion depth is the boundary with the sign place.The numerical value that reads from the measuring pipette place of this device is the normal volume of the right foot of animal.Survey twice continuously, ask its meansigma methods.Irritate the following medicine of stomach respectively for 3 treated animals: the solution that Herba Polygoni cymosi extract makes, hydrocortisone, normal saline, capacity is 1ml/100g.Behind the 30min in every Mus right hind centre of the palm to ankle joint direction inserting needle, subcutaneous injection 10% egg clear liquid 0.1ml/ is only.Thereafter per volume of measuring the Mus right hind in 0.5 hour respectively, continuous 3 times.
Experimental result (table 8) shows that Herba Polygoni cymosi extract has good antiinflammatory action (stronger than hydrocortisone).
Table 8 Herba Polygoni cymosi extract causes the influence of pedal swelling behind the rat to Ovum Gallus domesticus album
Annotate: * * represents and the poor heteropole of normal saline group remarkable (P＜0.01)
2. Herba Polygoni cymosi extract is to the analgesic activity (writhing method) of white mice:
Method and result: 40 of Kunming kind white mice, body weight 18-22g, ♀ ♂ half and half cuts the hair of white mice abdominal part, is divided into 2 groups at random, 20 every group.The administration group gavages Herba Polygoni cymosi extract 0.3g/10g, and matched group gavages the equivalent normal saline.30min after the administration, each Mus lumbar injection 0.6% acetum 0.2ml/ are only.The number of times and the time that occur writhing response behind observation and the record injection acetum in the 15-30min.Calculate the analgesia percentage rate of Herba Polygoni cymosi extract.The results are shown in Table 9.
The result shows: the white mice writhing response has the obvious suppression effect due to the Herba Polygoni cymosi extract Dichlorodiphenyl Acetate.
The influence of white mice writhing response due to table 9 Dichlorodiphenyl Acetate (x ± s)
| Group | Number of animals (only) | Drug level (%) | Turn round the body number of times in the 15min |
| The normal saline group | 20 | -- | ?41.45±7.75 |
| The Herba Polygoni cymosi extract group | 20 | 5 | ?18.02±4.33** |
3. mice caused by dimethylbenzene xylene auricle swelling degree test:
Method and result: get 50 of mices, male and female half and half are divided into 5 groups at random.Blank group gives the equivalent normal saline, small dose group gives Herba Polygoni cymosi extract solution 0.025ml/g, middle dosage group gives Herba Polygoni cymosi extract solution 0.05ml/g, high dose group gives Herba Polygoni cymosi extract solution 0.1ml/g, successive administration 5 days, 1 lumbar injection of matched group gives hydrocortisone 0.02mg/g.1h after the not inferior administration, 40 μ l dimethylbenzene are applied to the wide two sides of mouse right ear cause inflammation, left side ear is not painted with contrast, behind the 4h mouse anesthesia is put to death, cut ears and lay round auricle at same position respectively with the card punch of diameter 9mm, weigh, as the swelling degree, calculate swelling and suppress percentage rate with left and right sides auricle weight difference.The results are shown in Table 10:
The influence of table 10 Herba Polygoni cymosi extract mice auricle swelling (mg, X ± s)
The result shows: the Herba Polygoni cymosi extract xylol causes mice auricle swelling and has significant inhibitory effect, illustrates that this extract has good antiinflammatory action.
4. Herba Polygoni cymosi extract causes the influence test of rat temperature heating to yeast
Method and result: before the formal test, survey the rat rectal temperature every day 1 time, survey altogether 3 times, select body temperature to change 40 of the rats below 0.3 ℃, male and female half and half are divided into 5 groups at random by body temperature and sex: normal control group, positive controls (aspirin 0.1g/kg), the high, medium and low dosage group of Herba Polygoni cymosi extract, last is surveyed behind the body temperature with 20% yeast 1ml/100g, subcutaneous injection pyrogenicity, 3 hours gastric infusions after the pyrogenicity, 1 hour, 2 hours, 3 hours body temperature changes after the mensuration administration.The results are shown in Table 11:
Table 11 Herba Polygoni cymosi extract is to the influence of yeast pyrogenic action
| Group | Body temperature before the medicine | Behind the medicine 1 hour | Behind the medicine 2 hours | Behind the medicine 3 hours |
| The normal control group | 38.32±0.21 | 39.87±0.26 | 39.78±0.87 | 39.34±0.38 |
| Positive controls | 38.33±0.24 | 37.11±0.32** | 37.21±0.33** | 37.86±0.56* |
| Low dose group | 38.36±0.31 | 37.73±0.38** | 37.79±0.26** | 38.85±0.34 |
| Middle dosage group | 38.29±0.28 | 37.64±0.32** | 37.71±0.41** | 38.20±0.33 |
| High dose group | 38.31±0.27 | 37.77±0.34** | 37.81±0.37* | 39.13±0.26 |
Annotate: * P＜0.05 * * P＜0.01
The result shows: Herba Polygoni cymosi extract causes the rat temperature rising to yeast and has the effect of tangible reduction body temperature, illustrates that this extract has good refrigeration function.
Conclusion: the result by above four tests shows, Herba Polygoni cymosi extract has good analgesic, analgesia, antiinflammatory action, can be to gynaecopathia, particularly non-specific vulvitis, vulval ulcer, pruritus vulvae, vaginitis, cervicitis, pelvic inflammatory disease, amenorrhea, dysmenorrhea play good therapeutical effect; Simultaneously traumatology disease such as injury from falling down had therapeutical effect.
The specific embodiment:
Embodiments of the invention 1: the preparation of Herba Polygoni cymosi water extract:
Get Herba Polygoni cymosi aerial parts dry product 5kg, be ground into coarse powder, add 10 times of water gagings and decoct 2 times, 2 hours for the first time, 1.5 hours for the second time, filter, merging filtrate, being concentrated into 60 ℃, to survey relative densities be 1.30, puts coldly, adds ethanol and make that to contain the alcohol amount be 70%, standing over night, getting supernatant, to be evaporated to relative density be 1.25 thick paste, and 75 ℃ of drying under reduced pressure promptly get Herba Polygoni cymosi water extract 430g.
Embodiments of the invention 2: the preparation of Herba Polygoni cymosi ethanol extraction:
Get Herba Polygoni cymosi aerial parts dry product 5kg, be ground into coarse powder, add 8 times of amount alcohol reflux of 75% 2 times, each 2 hours, filter merging filtrate, decompression recycling ethanol, it is 1.25 thick paste that surplus liquid is concentrated into relative density, and drying under reduced pressure promptly gets Herba Polygoni cymosi ethanol extraction 410g.
Embodiments of the invention 3: the preparation of Herba Polygoni cymosi ligroin extraction:
After Herba Polygoni cymosi aerial parts dry product 5kg pulverizing, use the petroleum ether heating and refluxing extraction, use petroleum ether 30L at every turn, extract 3 times, merge extractive liquid,, reduction vaporization obtains ligroin extraction 370g to doing.
Embodiments of the invention 4: the preparation of Herba Polygoni cymosi ethyl acetate water solution extract:
After Herba Polygoni cymosi aerial parts dry product 5kg pulverizing, with ethyl acetate aqueous solution heating and refluxing extraction, use ethyl acetate aqueous solution 40L at every turn, extract 2 times, merge extractive liquid,, reduction vaporization obtains ethyl acetate water solution extract 370g to doing.
Embodiments of the invention 5: the preparation of Herba Polygoni cymosi chloroform extraction thing:
After Herba Polygoni cymosi aerial parts dry product 5kg pulverizing, extract with the chloroform diafiltration, use chloroform 25L at every turn, extract 4 times, merge extractive liquid,, reduction vaporization obtains chloroform extraction thing 250g to doing.
Embodiments of the invention 6: the pharmaceutical composition 1 that contains Herba Polygoni cymosi extract:
Take by weighing raw material by following proportioning: Herba Polygoni cymosi 200g, Galla Chinensis 150g, Fructus Mume 100g, Radix Sophorae Flavescentis 100g, Herba Senecionis Scandentis 100g.The above five tastes decoct with water 2 times, each 2 hours, filter, it is 1.20 (60 ℃) that filtrate is concentrated into relative density, and adding ethanol to concentration is 70%, standing over night is got supernatant, concentrates, drying under reduced pressure, dry extract was pulverized 160 orders, in extractum and PEG20001: and 1 ratio weighing, mix, grind well with colloid mill, be pressed into 1000 soft capsules.This preparation oral, each 4,3 times on the one.Be used for the treatment of gynaecopathia such as non-specific vulvitis, vulval ulcer, pruritus vulvae, vaginitis, cervicitis, pelvic inflammatory disease, amenorrhea or dysmenorrhea.
Embodiments of the invention 7: the pharmaceutical composition 2 that contains Herba Polygoni cymosi extract:
Take by weighing raw material by following proportioning: Herba Polygoni cymosi 60g, Radix Angelicae Sinensis 30-60g, Flos Carthami 30g, Olibanum 20-30g, Myrrha 20-30g, Sanguis Draxonis 20-30g, Fructus Carpesii 30g is ground into smalls, and boiled water dashes, and waits temperature and adds Chinese liquor 100mL and gavage, or drink sheet and fry in shallow oil soup and gavage.Be used for the treatment of traumatology diseases such as injury from falling down, bones and muscles pain.
Embodiments of the invention 8: Herba Polygoni cymosi extract single preparations of ephedrine:
Take by weighing Herba Polygoni cymosi extract 200g, vegetable oil 156.8g, Cera Flava 3.2g mixing, grind evenly,, make 1000, every 0.4g with the canned one-tenth soft capsule of pressing with colloid mill.This preparation oral, one time 4,3 times on the one.Be used for the treatment of pertussis, herpes zoster etc.
Claims (6)
1. the preparation method of Herba Polygoni cymosi extract, it is characterized in that: with the Herba Polygoni cymosi aerial parts dried and crushed of fresh collection, obtain the Herba Polygoni cymosi coarse powder, extract 2～4 times with ethanol or water reflux or diafiltration then, obtain Herba Polygoni cymosi extract behind the extracting solution concentrate drying; Specifically: it is an amount of to get Herba Polygoni cymosi, is ground into coarse powder, adds 10 times of water gagings and decocts 2 times, 2 hours for the first time, 1.5 hours for the second time, filter, merging filtrate, being concentrated into 60 ℃, to survey relative densities be 1.30, puts coldly, adds ethanol and make that to contain the alcohol amount be 70%, standing over night, getting supernatant, to be evaporated to relative density be 1.25 thick paste, and 75 ℃ of drying under reduced pressure promptly get the Herba Polygoni cymosi water extract.
2. the preparation method of Herba Polygoni cymosi extract, it is characterized in that: with the Herba Polygoni cymosi aerial parts dried and crushed of fresh collection, obtain the Herba Polygoni cymosi coarse powder, extract 2～4 times with ethanol or water reflux or diafiltration then, obtain Herba Polygoni cymosi extract behind the extracting solution concentrate drying; Specifically: it is an amount of to get Herba Polygoni cymosi, is ground into coarse powder, adds 8 times of amount alcohol reflux of 75% 2 times, each 2 hours, filter merging filtrate, decompression recycling ethanol, it is 1.25 thick paste that surplus liquid is concentrated into relative density, and drying under reduced pressure promptly gets the Herba Polygoni cymosi alcohol extract.
3. the Herba Polygoni cymosi extract preparation method is extracted the Herba Polygoni cymosi extract that obtains as claimed in claim 1 or 2.
4. the pharmaceutical composition that Herba Polygoni cymosi extract as claimed in claim 3 is formed is characterized in that: comprise Herba Polygoni cymosi extract and one or more pharmaceutical carriers as active component.
5. Herba Polygoni cymosi extract as claimed in claim 3 is used to prepare the medicine of the non-specific vulvitis of treatment, vaginitis, cervicitis, pelvic inflammatory disease, dysmenorrhea or vulval ulcer, pruritus vulvae, amenorrhea.
6. Herba Polygoni cymosi extract as claimed in claim 3 is used to prepare the medicine for the treatment of injury from falling down.
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| CN101549021B (en) * | 2009-06-03 | 2011-11-30 | 贵州师范大学 | Method for testing quality of perfoliate knotweed medicinal materials |
| CN101940623A (en) * | 2010-09-06 | 2011-01-12 | 范政 | Antibiotic and antiphlogistic medicinal composition and preparation method thereof |
| CN102764306B (en) * | 2012-08-15 | 2014-05-21 | 桂林医学院 | Application of perfoliote knotweed herb extract in preparation of anti-liver fibrosis drug |
| CN113476502A (en) * | 2021-08-16 | 2021-10-08 | 上海中医药大学附属曙光医院 | Periploca sepium extract, preparation method thereof and application of perfoliate sepium extract in preparation of non-alcoholic fatty liver disease or obesity drugs |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1147314C (en) * | 2002-11-13 | 2004-04-28 | 贵州和仁堂药业有限公司 | Medicine for treating gynecopathy |
| CN1552372A (en) * | 2003-06-03 | 2004-12-08 | 毛友昌 | Pills against gynecologic inflammation and their preparation |
| CN1785417A (en) * | 2005-11-16 | 2006-06-14 | 贵州安泰药业有限公司 | Chinese medicinal preparation for treating gynecopathy inflammation and its preparing process |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1147314C (en) * | 2002-11-13 | 2004-04-28 | 贵州和仁堂药业有限公司 | Medicine for treating gynecopathy |
| CN1552372A (en) * | 2003-06-03 | 2004-12-08 | 毛友昌 | Pills against gynecologic inflammation and their preparation |
| CN1785417A (en) * | 2005-11-16 | 2006-06-14 | 贵州安泰药业有限公司 | Chinese medicinal preparation for treating gynecopathy inflammation and its preparing process |
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| Title |
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| JP平3-220129A 1991.09.27 |
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