CN1939118A - Method for improving growth of taxol - Google Patents
Method for improving growth of taxol Download PDFInfo
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- CN1939118A CN1939118A CNA2005100448432A CN200510044843A CN1939118A CN 1939118 A CN1939118 A CN 1939118A CN A2005100448432 A CNA2005100448432 A CN A2005100448432A CN 200510044843 A CN200510044843 A CN 200510044843A CN 1939118 A CN1939118 A CN 1939118A
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Abstract
A method for promoting growth of enqlish yew is characterized by that its sunshine shielding rate is controlled according to the age of enqlish yew sapling and the water content in soil is controlled to 19-35% for keeping the soil in moist state.
Description
Technical field
The present invention relates to a kind of method that promotes growth of taxol.
Background technology
Novel high efficiency anti-tumor drug taxol is at first to be separated by the Dr Wani of U.S. triangle research institute to obtain from the bark of Ramulus et folium taxi cuspidatae of the Pacific Ocean, and it is newfound a kind of anti-cancer herb medicine after adriamycin, cis-platinum.Because it, has become the focus of world today's natural drug research in significant curative effect and the unique mechanism controlled in the cancer field.Because growth of taxol is slow, its contained bearing taxanes content is low, has greatly limited the supply of this similar drug.But quite big to its demand in the world, a large amount of felling that the extraction taxol causes from bark will bring threat to the species conservation and the area distribution of Chinese yew genus plants, destroy ecotope the most at last, cause the exhaustion of resource and the extinction of species.The Chinese yew genus plants resource problem has caused national governments and environment specialist's great attention, and people have turned to sight the artificial cultivation of actively developing Chinese yew genus plants in recent years.But nonstandard cultivation method has caused very serious problem.According to investigations, growth of taxol of more domestic local cultivations are in bad order, detect less than taxol in the branches and leaves, caused resource, man power and material's huge waste.Therefore, the content that how effectively to improve the growing ability of Chinese yew and bearing taxanes has become the problem that people extremely pay close attention to to improve industrialization cultivating economic benefit.
Summary of the invention
The present invention aims to provide a kind of method that promotes growth of taxol.
The present invention is based on such design concept: Chinese yew can grow under the canopy in woods as a kind of shade plant, but thin canopy, woods crack, woodland and border have the local Chinese yew of more sunlight can grow better.The canopy that crown density are high can suppress the growth of Chinese yew, is unfavorable for procreation and the renewal of its population, effect even can cause in the in imminent danger of this ground and disappear for a long time, and the growth of Chinese yew side shoot in the woods crack, woods window and diameter is all faster.Bearing taxanes has photodissociation and thermal instability, and relatively more responsive to high light, the content of the general bearing taxanes of Chinese yew plant under solar radiation is lower fully.And light is when too weak, though help the raising of bearing taxanes content, the amount of growth of plant is too little, is unfavorable for equally producing.The environment that Chinese yew hobby is moistening, growth of taxol gets badly under the dry environment, but easily ponding, viscid soil influences the respiration of Chinese yew, growth of taxol is very poor, easily death.Therefore can promote the growth of Chinese yew and the accumulation of bearing taxanes by adjusting to macroscopical ecological factor light and water.
The technical solution adopted in the present invention is: a kind of method that promotes growth of taxol is characterized in that: be the difference according to the growth year of Chinese yew little tree, adopt 10～100% the rate of shading; Keep ground moistening simultaneously, make soil moisture content 19%～35%.
To the annual Chinese yew little tree that has just brought out, adopt 100% the rate of shading above it.
To the Chinese yew little tree of life in 2 years, adopt 50%～70% the rate of shading above it.
To the Chinese yew little tree of life in 3 years to 5 years, adopt 35%～50% the rate of shading above it.
To the Chinese yew little tree of life in 6 years, adopt 10～30% the rate of shading above it.
When stem rot and root rot take place Chinese yew, in time water and execute 500～800 times of dilutions of 70% fenaminosulf, and apply lime.
Spray the bordeaux mixture of 1% concentration at the arid season in summer, autumn on the blade face of Chinese yew, spray per 15 days 1 time 3 times.
Keep the ridge-up bed draining good in rainy season, accomplish behind the pavement rain not ponding.
Adopt the mode of directly cultivating between col to shade to life in 2 years and above Chinese yew little tree.
To life in 2 years and above Chinese yew little tree, adopt the method for shading of the shady interplanting of woods, build the required environment that shades.
To the Chinese yew little tree of life in a year to 5 years, adopt the mode of taking the sunshade net to shade.
Described Chinese yew is Taxus x media or southern enqlish yew.
Through the inventor to different illumination intensity and soil water content experimental results show that repeatedly to the influence of cultivating Chinese yew little tree bearing taxanes total amount, the present invention can promote the growth of Chinese yew and the accumulation of bearing taxanes by the adjusting to macroscopical ecological factor light and water, avoid producing owing to the improper heavy losses that bring of the regulation and control of light and water, the extensive industrialization cultivation of Chinese yew is had important directive significance, have a extensive future.
Description of drawings
Fig. 1 is three the experimental group taxols handling with different illumination intensity and the block diagram of 10-deacylated tRNA Ji Bakading III output.
Fig. 2 is three the experimental group taxols handling with the different soils moisture and the block diagram of 10-deacylated tRNA Ji Bakading III output.
Nine the experimental group taxols that Fig. 3 handles with different illumination intensity and soil water content and the block diagram of 10-deacylated tRNA Ji Bakading III output.
Embodiment
Embodiment 1,3 years living Taxus x media treelets 1 meter are adopted 40 ± 5% shade above it, promptly adopt the mode of taking the sunshade net, 1 meter directly over little tree, set up special-purpose black sunshade net with support, can regulate light transmittance by the size of adjusting the sunshade net meshe, thereby reach the effect of shading of respective degrees.Soil adopts chiltern, the chesson that is beneficial to growth of taxol, keep ground moistening, the moisture content of at least 5 points of random measurement when at every turn monitoring remained on about 20% the soil moisture content of each point with soil water content analyzer monitoring soil moisture 1 time in per two days.At the arid season in summer and autumn, in time pour water, keep ground moistening.Because high temperature, strong sunshine, arid often cause Chinese yew to produce leaf blight and pestalotiopsis funerea, therefore except shading, also to adopt method control toward the foliage-spray bordeaux mixture.General 1% the concentration that adopts of bordeaux mixture sprays per 15 days 1 time 3 times.Regular meeting caused stem rot and root rot when rainwater was excessive, also will be early, in good time to its control.Generally execute 70% fenaminosulf 500-800 times dilution, and spread fertilizer over the fields lime, prevent that disease from spreading to water.Should keep the ridge-up bed draining good in rainy season, accomplish behind the pavement rain not ponding, guarantee that the ridge-up bed gas permeability of soil can be good.
When the mode that the sunshade net is taken in employing was shaded, the distance between sunshade net and little tree and the area that shades were different because of the age of tree.The mode that also can utilize the shady interplanting of woods or directly cultivate between col of shading is built the required rate of shading.
Table 1 is the biomass (mean value ± standard error % with every little tree in each experimental group of different illumination intensity processing; N=20), the degree of each experimental group taxol, 10-deacylated tRNA Ji Bakading III and output (mean value ± standard error %; N=3).Total biomass dry weight estimated value=every group of Chinese yew biomass fresh weight * biomass done/the fresh weight ratio.
| Sequence number | Light transmittance | Average fresh weight (g) during the experiment beginning | Average fresh weight (g) after two months | The degree of taxol, 10-deacylated tRNA Ji Bakading III | The pre-output (mg) of taxol, 10-deacylated tRNA Ji Bakading III |
| A1 | 20±5％ | 4.09±0.96 | 19.00±6.50 | 0.0136±0.0004％ 0.0247±0.0010％ | 2.584 4.693 |
| A2 | 40±5％ | 4.05±1.05 | 23.81±8.81 | 0.0117±0.0007％ 0.0216±0.0013％ | 2.786 5.143 |
| A3 | 100％ | 4.05±1.10 | 12.02±5.37 | 0.0061±0.0005％ 0.0187±0.0008％ | 0.733 2.248 |
Table 1
Fig. 1 is seen in three the experimental group taxols handling with different illumination intensity and the contrast of 10-deacylated tRNA Ji Bakading III output.The difference of respectively organizing as seen from Figure 1 between the gross yield has reached utmost point significance level, with light intensity (1813.4 ± 226.7MJm-2year-1) the processing output maximums of 40 ± 5% light transmittances.
Table 2 is the biomass (mean ± S.D.% with every little tree in each experimental group of different soils water content processing; N=20), degree and the output (mean value ± S.D.% of each experimental group taxol, 10-deacylated tRNA Ji Bakading III; N=3).Total biomass dry weight estimated value=every group of Chinese yew biomass fresh weight * biomass done/the fresh weight ratio.
| Sequence number | Soil water content | Average fresh weight (g) during the experiment beginning | Average fresh weight (g) after two months | The degree of taxol, 10-deacylated tRNA Ji Bakading III | The pre-output (mg) of taxol, 10-deacylated tRNA Ji Bakading III |
| B1 | 20.10±0.62％ | 3.57±1.09 | 13.27±3.86 | 0.57±0.04 1.73±0.07 | 0.756 2.296 |
| B2 | 13.51±0.53％ | 3.63±1.05 | 9.86±3.77 | 0.60±0.06 | 0.591 |
| 1.74±0.08 | 1.716 | ||||
| B3 | 7.21±0.34％ | 3.62±1.09 | 7.10±1.83 | 0.62±0.04 1.79±0.05 | 0.440 1.271 |
Table 2
Fig. 2 is seen in three the experimental group taxols handling with the different soils moisture and the contrast of 10-deacylated tRNA Ji Bakading III output.The difference of respectively organizing as seen from Figure 2 between the gross yield has reached utmost point significance level, and the soil water content with 20.10 ± 0.62% is handled the output maximum.
Table 3 is average biomass (the mean+SD % with every little tree in each cross-over experiment group of different illumination intensity and soil moisture content processing; N=20), degree and output (the mean+SD .% of each experimental group taxol, 10-deacylated tRNA Ji Bakading III; N=3).Total biomass dry weight estimated value=every group of Chinese yew biomass fresh weight * biomass done/the fresh weight ratio.
| Sequence number | Light transmittance | Soil water content | Average fresh weight (g) during the experiment beginning | Average fresh weight (g) after two months | The degree of taxol, 10-deacylated tRNA Ji Bakading III | Total dry biomass heavy (g) of |
| C1 | ||||||
| 20±5％ | 20.10±0.62 ％ | 5.70±0.73 | 28.08±3.61 | 0.0245±0.0006％ 0.0134±0.0005％ | 226.32±4.3 8 | |
| C2 | 20±5％ | 13.51±0.53 ％ | 5.64±0.76 | 21.19±2.79 | 0.0247±0.0012％ 0.0138±0.0006％ | 170.79±3.3 1 |
| C3 | 20±5％ | 7.21±0.34％ | 5.78±0.66 | 16.57±1.68 | 0.0265±0.0007％ 0.0141±0.0004％ | 133.55±2.5 8 |
| C4 | 40±5％ | 20.10±0.62 ％ | 5.72±0.69 | 37.28±4.85 | 0.0219±0.0014％ 0.0109±0.0007％ | 300.48±5.8 1 |
| | 40±5％ | 13.51±0.53 ％ | 5.67±0.59 | 26.40±3.41 | 0.0221±0.0010％ 0.0115±0.0009％ | 212.78±4.1 2 |
| C6 | 40±5％ | 7.21±0.34％ | 5.63±0.97 | 17.62±2.54 | 0.0230±0.0007％ 0.0122±0.0004％ | 142.02±2.7 5 |
| C7 | 100％ | 20.10±0.62 ％ | 5.74±0.61 | 18.44±2.25 | 0.0179±0.0009％ 0.0058±0.0007％ | 148.63±2.8 8 |
| C8 | 100％ | 13.51±0.53 ％ | 5.77±0.56 | 13.63±1.44 | 0.0185±0.0008％ 0.0063±0.0004％ | 109.86±2.1 3 |
| C9 | 100％ | 7.21±0.34％ | 5.84±0.64 | 8.89±1.10 | 0.0189±0.0014％ 0.0066±0.0005％ | 71.65±1.39 |
Table 3
Fig. 3 is seen in nine the experimental group taxols handling with different illumination intensity and soil water content and the contrast of 10-deacylated tRNA Ji Bakading III output.As seen from Figure 3, each organizes the significant difference between the gross yield, with 40 native 5% light transmittances, and output maximum when promptly light intensity is the soil water content associated treatment of 1813.4 ± 226.7MJm-2year-1 and 20.10 ± 0.62%.
The cross-over experiment result of different illumination intensity and soil water content shows: by regulating ecological factor and utilizing the synergy between the different ecological factor can reach the purpose that improves bearing taxanes output on producing.
Embodiment 2, sowing southern enqlish yew seed in chiltern, loose soil, 100% processing of shading is adopted at 1 meter above the seedling of cultivating, keep ground moistening, per two days with soil water content analyzer monitoring soil moisture 1 time, the moisture content of at least 5 points of random measurement remains on about 30% the soil moisture content of each some during each the monitoring.Points for attention during other cultivation are identical with embodiment 1.Adopt 75% to shade in same place to same seedling, compare, the results are shown in Table 4, table 5 with adopting 100% growing state that shades the seedling handled with 50% these two kinds of different cover degrees.
Table 4: different cover degrees are shaded influences variance analysis (Fq check) to the southern enqlish yew seedling growth
| The processing of shading | Height of seedling (cm) | Leading thread (cm) | The hat width of cloth (cm) |
| 100% | 25 | 0.33 | 19.40 |
| 75% shades | 18.40 | 0.28 | 17.80 |
| 50% shades | 16.80 | 0.25 | 17.50 |
Table 5: different cover degrees are shaded to the influence of southern enqlish yew seedling growth height
| The processing of shading | I level seedling | II level seedling | III level seedling | IV level seedling |
| 100% shades | 2(1％) | 50(25％) | 98(49％) | 50(25％) |
| 75% shades | 0(0％) | 0(0％) | 6(3％) | 194(97％) |
| 50% shades | 0(0％) | 0(0％) | 2(1％) | 198(99％) |
I level seedling: height＞40cm ground stem＞0.40cm; II level seedling: 40cm＞height＞30cm 0.40cm＞ground stem＞0.30cm; III level seedling: 30cm＞height＞20cm 0.30cm＞ground stem＞0.20cm; IV level seedling: height＜20cm ground stem＜0.20cm
Annotate: the percentage in table 5 bracket is respectively the ratio that nursery stock strain numbers at different levels account for total nursery stock number.
Embodiment 3, in lanceolata forest, adopt the mode of the shady interplanting of woods to plant 3 years living southern enqlish yew little trees, build 50% the environment maintenance ground moistening that shades, per two days with soil water content analyzer monitoring soil moisture 1 time, the moisture content of at least 5 points of random measurement remains on about 25% the soil moisture content of each some during each the monitoring.Points for attention during other cultivation are identical with embodiment 1.Plant in the same place height, 3 years consistent with the hat width of cloth living southern enqlish yew little trees of leading thread, adopt full exposure respectively, the 75% two kinds of different light treatment of shading, compare with adopting 50% growing state that shades the southern enqlish yew little tree of handling, the investigation different illumination intensity is to the influence of southern enqlish yew little tree growth, and the contrast situation sees Table 6.
Table 6: different cover degrees are to the investigation result that influences of the southern enqlish yew sapling up-growth of life in 3 years
| The processing of sheltering from heat or light | Height of seedling (m) | Leading thread (cm) | The hat width of cloth (m) | Lethality (%) |
| Full exposure | 0.51(0％) | 0.87(0％) | 0.43(0％) | 50 |
| 75% shades | 1.14(123％) | 1.67(92％) | 0.75(74％) | 0 |
| 50% shades | 1.46(186％) | 2.34(169％) | 1.6(272％) | 0 |
Annotate: the growth rate that the percentage in table 6 bracket is compared with nursery stock under the full exposure condition for every index of the different nursery stocks of handling of shading.
Claims (12)
1, a kind of method that promotes growth of taxol is characterized in that: be the difference according to the growth year of Chinese yew little tree, adopt 10～100% the rate of shading; Keep ground moistening simultaneously, make soil moisture content 19%～35%.
2, a kind of method that promotes growth of taxol according to claim 1 is characterized in that: to the annual Chinese yew little tree that has just brought out, adopt 100% the rate of shading above it.
3, a kind of method that promotes growth of taxol according to claim 1 is characterized in that: to the Chinese yew little tree of life in 2 years, adopt 50%～70% the rate of shading above it.
4, a kind of method that promotes growth of taxol according to claim 1 is characterized in that: to the Chinese yew little tree of life in 3 years to 5 years, adopt 35%～50% the rate of shading above it.
5, a kind of method that promotes growth of taxol according to claim 1 is characterized in that: to the Chinese yew little tree of life in 6 years, adopt 10～30% the rate of shading above it.
6, according to claim 1 or 2 or 3 or 4 described a kind of methods that promote growth of taxol, it is characterized in that: when stem rot and root rot take place Chinese yew, in time water and execute 500～800 times of dilutions of 70% fenaminosulf, and apply lime.
7, according to claim 1 or 2 or 3 or 4 described a kind of methods that promote growth of taxol, it is characterized in that: spray the bordeaux mixture of 1% concentration at the arid season in summer, autumn on the blade face of Chinese yew, spray per 15 days 1 time 3 times.
8, according to claim 1 or 2 or 3 or 4 described a kind of methods that promote growth of taxol, it is characterized in that: keep the ridge-up bed draining good in rainy season, accomplish behind the pavement rain not ponding.
9, according to claim 3 or 4 or 5 described a kind of methods that promote growth of taxol, it is characterized in that: the described method of shading is to adopt the method for shading of the shady interplanting of woods, builds the required environment that shades.
10, according to claim 3 or 4 or 5 described a kind of methods that promote growth of taxol, it is characterized in that: the described method of shading is to adopt the directly mode of cultivation between col.
11, according to claim 2 or 3 or 4 described a kind of methods that promote growth of taxol, it is characterized in that: the described method of shading is to adopt the mode of taking the sunshade net.
12, according to claim 1 or 2 or 3 or 4 or 5 described a kind of methods that promote growth of taxol, it is characterized in that: described Chinese yew is Taxus x media or southern enqlish yew.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810098A (en) * | 2010-04-23 | 2010-08-25 | 北京阿恩替生物科技有限公司 | Method for planting Chinese yew |
| CN102812842A (en) * | 2012-09-14 | 2012-12-12 | 江苏红豆杉生物科技股份有限公司 | Greenhouse maintenance method of taxus mairei bonsai |
| CN103168594A (en) * | 2013-03-26 | 2013-06-26 | 四川祥光农业科技开发有限公司 | Taxus media cultivation method |
-
2005
- 2005-09-29 CN CNA2005100448432A patent/CN1939118A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810098A (en) * | 2010-04-23 | 2010-08-25 | 北京阿恩替生物科技有限公司 | Method for planting Chinese yew |
| CN102812842A (en) * | 2012-09-14 | 2012-12-12 | 江苏红豆杉生物科技股份有限公司 | Greenhouse maintenance method of taxus mairei bonsai |
| CN102812842B (en) * | 2012-09-14 | 2014-02-12 | 江苏红豆杉生物科技股份有限公司 | Greenhouse maintenance method of taxus mairei bonsai |
| CN103168594A (en) * | 2013-03-26 | 2013-06-26 | 四川祥光农业科技开发有限公司 | Taxus media cultivation method |
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