The specific embodiment
The preparation of embodiment 1 Radix Aconiti Lateralis water extract
Get Radix Aconiti Lateralis medical material 13.50kg, with water logging bubble 30min, add to 10 times of water gagings and decoct, decocting time is got respectively and is boiled back 6h, add 10 times of water gagings the 2nd time, decocted 4 hours, and filtered merging filtrate, be evaporated to 1.02 ~ 1.05 (60 ℃), be drying to obtain the 2.51Kg extract, inlet temperature is: 90~100 ℃, leaving air temp is: 60~65 ℃.
The preparation of embodiment 2 Radix Aconiti Lateralis Preparata water extract
Get Radix Aconiti Lateralis medical material 12.50kg, with water logging bubble 30min, add to 9 times of water gagings and decoct, decocting time is got respectively and is boiled back 5.5h, add 9 times of water gagings the 2nd time, decocted 3.5 hours, and filtered merging filtrate, be evaporated to 1.02 ~ 1.05 (60 ℃), be drying to obtain the 2.25Kg extract, inlet temperature is: 90~100 ℃, leaving air temp is: 60~65 ℃.
The preparation of embodiment 3 medicinal granules of the present invention
Get Radix Aconiti Lateralis medical material 11.50kg,, add to 11 times of water gagings and decoct with water logging bubble 30min, decocting time is got respectively and is boiled back 6.5h, adds 11 times of water gagings, decocts 4.5 hours for the 2nd time, filter, merging filtrate is evaporated to 1.02 ~ 1.05 (60 ℃), be drying to obtain the 2.08Kg extract, inlet temperature is: 90~100 ℃, leaving air temp is: 60~65 ℃, extract adds starch and granulates, granulate promptly gets granule.
The preparation of embodiment 4 Radix Aconiti Lateralis Preparata extracts
Get Radix Aconiti Lateralis Preparata medical material 12.35kg, with water logging bubble 30min, add to 10 times of water gagings and decoct, decocting time is got respectively and is boiled back 5h, add 8 times of water gagings the 2nd time, decocted 3 hours, and filtered merging filtrate, be evaporated to 1.02 ~ 1.05 (60 ℃), be drying to obtain the 2.03Kg extract, inlet temperature is: 90~100 ℃, leaving air temp is: 60~65 ℃.
The preparation of embodiment 5 Radix Aconiti Lateralis Preparata extracts
Get Radix Aconiti Lateralis Preparata medical material 11.50kg, with water logging bubble 30min, add to 9 times of water gagings and decoct, decocting time is got respectively and is boiled back 4.5h, add 7 times of water gagings the 2nd time, decocted 2.5 hours, and filtered merging filtrate, be evaporated to 1.02 ~ 1.05 (60 ℃), be drying to obtain the 2.03Kg extract, inlet temperature is: 90~100 ℃, leaving air temp is: 60~65 ℃.
The preparation of embodiment 6 medicinal tablets of the present invention
Get Radix Aconiti Lateralis Preparata medical material 12.35kg,, add to 11 times of water gagings and decoct with water logging bubble 30min, decocting time is got respectively and is boiled back 5.5h, adds 9 times of water gagings, decocts 3.5 hours for the 2nd time, filter, merging filtrate is evaporated to 1.02 ~ 1.05 (60 ℃), be drying to obtain the 2.03Kg extract, inlet temperature is: 90~100 ℃, leaving air temp is: 60~65 ℃, the extract of preparation adds adjuvant, system granule, tabletting, promptly.
The preparation of embodiment 7 Radix Aconiti Lateralis Preparata extracts
Get Radix Aconiti Lateralis Preparata medical material 12.35kg, with water logging bubble 30min, add to 10 times of water gagings and decoct, decocting time is got respectively and is boiled back 4h, add 10 times of water gagings the 2nd time, decocted 4 hours, and filtered merging filtrate, be evaporated to 1.02 ~ 1.05 (60 ℃), be drying to obtain the 2.03Kg extract, inlet temperature is: 90~100 ℃, leaving air temp is: 60~65 ℃.
The preparation that embodiment 8 Radix Aconiti Lateralis Preparatas extract
Get Radix Aconiti Lateralis Preparata medical material 11kg, with water logging bubble 30min, add to 11 times of water gagings and decoct, decocting time is got respectively and is boiled back 4.5h, add 11 times of water gagings the 2nd time, decocted 4.5 hours, and filtered merging filtrate, be evaporated to 1.02 ~ 1.05 (60 ℃), be drying to obtain the 2Kg extract, inlet temperature is: 90~100 ℃, leaving air temp is: 60~65 ℃.
The preparation of embodiment 9 medicine capsules of the present invention
Get Radix Aconiti Lateralis Preparata medical material 12.35kg,, add to 9 times of water gagings and decoct with water logging bubble 30min, decocting time is got respectively and is boiled back 3.5h, adds 9 times of water gagings, decocts 3.5 hours for the 2nd time, filter, merging filtrate is evaporated to 1.02 ~ 1.05 (60 ℃), is drying to obtain the 2.03Kg extract, inlet temperature is: 90~100 ℃, leaving air temp is: 60~65 ℃, the extract of preparation adds starch system granule, encapsulated, makes capsule.
The method of quality control of embodiment 10 drug extracts of the present invention
[character] this product be pale brown color to brown particle, feeble QI, mildly bitter flavor is puckery.
[discriminating] gets this product 2g, and porphyrize is put in the tool plug conical flask, add ammonia solution 5ml, add ether 3ml, supersound extraction 10 minutes, jolting 20 minutes, filter, filtrate is put in the separatory funnel, adds the 0.25mol/L sulfuric acid solution, and jolting is extracted, divide and get acid solution, the photograph spectrophotography (" appendix VA of Chinese pharmacopoeia version in 2000) measure.At 231nm and 274nm wavelength place absorption maximum is arranged.
[inspection] should meet every regulation relevant under the granule item (" appendix IC of Chinese pharmacopoeia version in 2000).
[limit examine] photograph spectrophotography (" appendix VA of Chinese pharmacopoeia version in 2000) measure.
1, the drafting precision of standard curve takes by weighing aconitine reference substance 20mg and adds an amount of anhydrous alcohol solution, moves in the 10ml measuring bottle, adds dehydrated alcohol to scale, shakes up, promptly.Precision is measured aconitine titer 0,0.50,1.00,1.25,1.50,2.00,2.50ml, puts in the 25ml volumetric flask, adds ethanol to 2.5ml, the alkaline oxammonium hydrochloride. test solution 1.5ml of accurate adding, water-bath is 10 minutes in 60 ~ 65 ℃, takes out, and puts cold, add 13ml ferric perchlorate test solution, shake up, placed 5 minutes, the accurate perchloric acid test solution 8ml that adds, shake up, be settled to scale with ferric perchlorate's test solution.Make reference with blank, under 520nm, measure its absorbance, handle with the mapping of trap value and through rectilinear regression with biological alkali number, must linear equation.
2, the preparation of need testing solution respectively precision take by weighing 2 minimum package contents, add ammonia solution 5ml, with ether 50ml continuous jolting 1 hour, merceration spends the night, reuse ether 50ml divides 3 washings, filters merging filtrate and washing liquid, the low temperature evaporate to dryness, the 5ml that adds diethyl ether dissolving volatilizes, and adds ethanol 2.5ml dissolving, put in the 25ml volumetric flask, calculate and try to achieve total alkaloid content from " the alkaline oxammonium hydrochloride. of accurate adding " to " under 520nm, measuring its absorbance "
Every bag of 2g of this product contains Radix Aconiti Lateralis in aconitine (C34H47NO11), must not be higher than 1.5mg.
Every bag of 4g of this product contains Radix Aconiti Lateralis Preparata in aconitine (C34H47NO11), must not be higher than 1.0mg.
Every bag of 4g of this product contains Radix Aconiti Lateralis Preparata in aconitine (C34H47NO11), must not be higher than 1.5mg.
[assay] photograph spectrophotography (" appendix VA of Chinese pharmacopoeia version in 2000) measure.
1, the mark drafting precision that pushes away curve takes by weighing aconitine reference substance 10mg and adds an amount of chloroform dissolving, moves in the 100ml measuring bottle, adds chloroform to scale, shakes up, promptly.Precision is measured aconitine titer 0,0.50,1.00,1.25,1.50,2.00,2.50ml, places separatory funnel respectively, and the accurate atmosphere that adds is imitative to 20.0ml.With first part be blank, respectively add 10mlpH6.1 ± 0.1 bromocresol green dyestuff then, shake well left standstill 1 hour, chloroform solution is filtered in the 25ml volumetric flask and with chloroform and is diluted to scale.In 410nm wavelength place, measure trap blank, test liquid respectively.Handle with the mapping of biological alkali number and trap value and through rectilinear regression, linear equation.
2, the preparation of need testing solution respectively precision take by weighing single minimum package content, it is standby to press differential method, replace chloroform with E-C (3: 1) mixed liquor, put in the evaporating dish, water-bath low temperature evaporate to dryness adds diethyl ether-chloroform (3: 1) mixed liquor 5ml evaporate to dryness, add chloroform and make dissolving, standardize solution is in 1ml, and each mensuration is got the solution of 0.1ml respectively, is transferred in the separatory funnel, add chloroform to 20ml, promptly.Measure to " 410nm wavelength place " from " adding 10mlpH6.1 ± 0.1 bromocresol green dyestuff " in accordance with the law, calculate and try to achieve total alkaloid content:
Every bag of 2g of this product contains Radix Aconiti Lateralis in aconitine (C34H47NO11), must not be less than 3.6mg.
Every bag of 4g of this product contains Radix Aconiti Lateralis Preparata in aconitine (C34H47NO11), must not be less than 3.5mg
Every bag of 4g of this product contains Radix Aconiti Lateralis Preparata in aconitine (C34H47NO11), must not be less than 3.0mg.
Below method by the test of pesticide effectiveness beneficial effect of the present invention is further described.
The screening test of test example 1 Radix Aconiti Lateralis Preparata extract extraction process different parameters of the present invention
(1) screening test
1, experiment material
1.1 medicine
Radix Aconiti Lateralis Preparata (Radix Aconiti Lateralis, Radix Aconiti Lateralis Preparata, RADIX ACONITI LATERALIS PREPARATA): be subjected to the reagent thing,, identify that through this school crude drug teaching and research room is accredited as the daughter root of Aconitum carmichjaelii Debx. Aconitum carmichaeli Debx., gathers then available from river, Sichuan oil.Soak 30min with 3 times of water gagings, add to 10 times of water gagings and decoct, decocting time is got 7 time points such as boiling back 15min, 30min, 60min, 120min, 3h, 4h, 6h respectively, and decocting the concentrating under reduced pressure that finishes is that every ml contains crude drug in whole 3g.According to uniform designs table, 7 time points that Radix Aconiti Lateralis Preparata (Radix Aconiti Lateralis, Radix Aconiti Lateralis Preparata, RADIX ACONITI LATERALIS PREPARATA) decocts 15min, 30min, 60min, 120min, 3h, 4h, 6h are got 24 times, 2 times, 48 times, 12 times, 1 times, 36 times and 6 times of clinical dosage (15g/60kg.d-1) successively, and facing the time spent, to be mixed with 60%, 7.5%, 120%, 30%, 2.5%, 90%, 15% medicinal liquid respectively with distilled water standby.
SHENFU ZHUSHEYE: Sanjiu Pharmaceutical Industry Co., Ltd., Ya'an City produces, authentication code: the accurate word Z51020664 of traditional Chinese medicines, product batch number: 040513.Specification: 10ml/ props up.
Dexamethasone acetate: positive drug, Zhejiang Province XianJu Pharmacy stock Co., Ltd produces.Authentication code: the accurate word H33020822 of traditional Chinese medicines.Product batch number: 040209.Specification: 0.75mg/ sheet.It is standby to be mixed with 0.03% medicinal liquid with distilled water during experiment.
Tramadol: positive drug, Shijiazhuang Pharmaceutical Group Co Ltd produces.Authentication code: the accurate word H10960106 of traditional Chinese medicines.Product batch number: 031003.Specification: 50mg/ sheet.It is standby to be mixed with 1% medicinal liquid with distilled water during experiment.
The TONGBIAN LING capsule: positive drug, Qili Pharaceutical Co., Ltd., Sichuan produces.Authentication code: the accurate word Z51020262 of traditional Chinese medicines.Product batch number: 040201.Specification: 12 * 2 plates/box.It is standby to be mixed with 5% medicinal liquid with distilled water during experiment.
JINGUI SHENQI WAN: positive drug, the Beijing Tongrentang Technology Development Co.ltd. Pharmaceutical Factory produces.Authentication code: the accurate word Z11020417 of traditional Chinese medicines.Product batch number: 040811.Specification: 360/bottle.It is standby to be mixed with 33.33% JINGUI SHENQI WAN medicinal liquid with distilled water during experiment.
The hydroxyurea capsule: the modeling medicine, Shanxi Ante Biological Pharmaceutical Co., Ltd. produces.Authentication code: the accurate word H14023012 of traditional Chinese medicines.Product batch number: 20030702.Specification: 0.25 * 12 * 2 plates/box.It is standby to be mixed with 3.75% hydroxyurea medicinal liquid with distilled water during experiment.
Hydrocortisone injection: the modeling medicine, Tianjin gold credit aminoacid company limited is produced.Authentication code: the accurate word H12020887 of traditional Chinese medicines.Product batch number: 040310.Specification: 100mg/20ml/ props up.
The hydrochloric acid propafenone: the modeling medicine, Mingxing Pharmaceuticals Co., Ltd., Guangzhou City produces.Authentication code: the accurate word H4402049 of traditional Chinese medicines.Product batch number: MC3201.Specification: 35mg/10ml/ props up.
1.2 animal
Kunming mouse is provided laboratory animal production licence number: 04-11 by Chengdu University of Traditional Chinese Medicine Animal Experimental Study center.The SD rat is provided laboratory animal production licence number: 04-11 by Chengdu University of Traditional Chinese Medicine Animal Experimental Study center.The quarantine back is standby.
1.3 instrument
BL-420E biological function experimental system, Chengdu TME Technology Co., Ltd.'s development.RB-200 type intelligence hot-plate instrument, Chengdu TME Technology Co., Ltd. produces.
2, experimental technique
2.1 warming YANG test
2.1.1 deficiency of heart-YANG test
Zhang Zibin, Tsung O.Cheng, Zhang Yuchuan. congestive heart failure. Beijing: scientific and technical literature publishing house, 2002,204
2.1.2 insufficiency of kidney-YANG test
2.1.2.1 Radix Aconiti Lateralis insufficiency of kidney-YANG test
Liao Jinmin, Wu Tie. hydroxyurea causes the experimentation of " yang deficiency " rat metabolic alterations. and journal, 1994 are dissected in Guangdong; 16 (1): 24-28
2.1.2.2 Radix Aconiti Lateralis Preparata insufficiency of kidney-YANG test
2.1.2.2.1 swimming test
The hole is written, Lei Chunli, and Lv Wenwei, etc. astragaloside is to the effect [J] of experimental heart failure. Norman Bethune Medical University's journal, 1994,20 (2): 125
2.1.2.2.2 pole-jump test
The hole is written, Lei Chunli, and Lv Wenwei, etc. astragaloside is to the effect [J] of experimental heart failure. Norman Bethune Medical University's journal, 1994,20 (2): 125
2.1.2.3 RADIX ACONITI LATERALIS PREPARATA insufficiency of kidney-YANG test
2.1.2.3.1 swimming test
Get 120 of the Kunming mouses (male and female half and half) of body weight 20-24g, be divided into blank group (n=10), model control group (n=14), positive controls (n=12), RADIX ACONITI LATERALIS PREPARATA 15min group (n=12), 30min group (n=12), 1h group (n=12), 2h group (n=12), 3h group (n=12), 4h group (n=12), 6h at random and organize (n=12).The same 2.1.2.2.1 of medication and date processing.
2.1.2.3.2 pole-jump test
Get 120 of the Kunming mouses (male and female half and half) of body weight 20-24g, be divided into blank group (n=10), model control group (n=14), positive drug group (n=12), RADIX ACONITI LATERALIS PREPARATA 15min group (n=12), 30min group (n=12), 1h group (n=12), 2h group (n=12), 3h group (n=12), 4h group (n=12), 6h at random and organize (n=12).The same 2.1.2.2.2 of medication and date processing.
2.1.3 yang deficiency constipation test
2.1.3.1 Radix Aconiti Lateralis yang deficiency constipation test
Get 158 of body weight 20-24g Kunming mouses (male and female half and half), be divided into blank group (n=10), model group (n=20), positive drug group (n=16), Radix Aconiti Lateralis 15min group (n=16), 30min group (n=16), 1h group (n=16), 2h group (n=16), 3h group (n=16), 4h group (n=16) at random, 6h organizes (n=16).Except that the blank group, each group is all irritated stomach Shanxi white vinegar (Shanxi Province beautiful and occupy the production of old vinegar company limited, acidity 3.5g/100ml), and first dosage 15ml/kg played 10ml/kg.d-1, continuous 9 days on 2nd.The 7th, give 0 ℃ of active carbon frozen water 25ml/kg.d-1 of 10% when irritating the stomach white vinegar, continuous 3 days.Each experimental group modeling is irritated stomach distilled water, 5% TONGBIAN LING capsule medicinal liquid, 60%, 7.5%, 120%, 30%, 2.5%, 90%, 15% Radix Aconiti Lateralis medicinal liquid 10ml/kg.d-1, continuous 9 days respectively by group simultaneously.The 8th, water 24h was can't help in the animal fasting.Behind last administration 1h, mice is put into metabolic cage respectively, write down defecation grain number and first grain defecation time (incubation period) in each treated animal 3h.After observation finishes, each is organized mouse stomach nutrition and sticks with paste (red ink 125ml, sodium carboxymethyl cellulose 5g, starch 5g, milk powder 10g, white sugar 5g) 15ml/kg, put to death after 15 minutes, measure small intestinal total length and nutrition and stick with paste propelling length, calculate intestinal propelling rate (nutrition is stuck with paste and advanced length/small intestinal total length * 100%).Experimental data is handled with the SPSS11.0 statistical software, and regression analysis adopts uniform Design 1.0 special-purpose softwares to handle.
2.1.3.2 Radix Aconiti Lateralis Preparata yang deficiency constipation test
Get 130 of 20-24g Kunming mouses (male and female half and half), be divided into blank group (n=13), model control group (n=13), positive group (n=13), Radix Aconiti Lateralis Preparata 15min group (n=13), 30min group (n=13), 60min group (n=13), 1h group (n=13), 2h group (n=13), 3h group (n=13), 4h group (n=13) at random, 6h organizes (n=13).The same 2.1.3.1 of medication and date processing.
2.1.3.3 RADIX ACONITI LATERALIS PREPARATA yang deficiency constipation test
The same 2.1.3.1 of experimental technique and date processing.
2.2 antiinflammatory test
2.2.1 dimethylbenzene auricle edema test
Xu Shuyun, Bian Rulian, old repairing. pharmacological experimental methodology (the 2nd edition). Beijing: People's Health Publisher, 1991,719
2.2.2 Ovum Gallus domesticus album pedal swelling test
Xu Shuyun, Bian Rulian, old repairing. pharmacological experimental methodology (the 3rd edition). Beijing: People's Health Publisher, 2002,911
2.2.3 Oleum Tiglii granuloma test
Xu Shuyun, Bian Rulian, old repairing. pharmacological experimental methodology (the 3rd edition). Beijing: People's Health Publisher, 2002,918
2.3 analgesic test
2.3.1 writhing test
Xu Shuyun, Bian Rulian, old repairing. pharmacological experimental methodology (the 3rd edition). Beijing: People's Health Publisher, 2002,882
2.3.2 hot plate test
Xu Shuyun, Bian Rulian, old repairing. pharmacological experimental methodology (the 3rd edition). Beijing: People's Health Publisher, 2002,885
3, experimental result
3.1 warming YANG test
3.1.1 deficiency of heart-YANG test
3.1.1.1 influence to heart rate
After the model group rat gave propafenone 10.5mg/kg modeling, heart rate significantly slowed down immediately, reduced to minimumly in the several seconds, slowly rose thereafter, continued tens of minutes, with the blank group utmost point significant difference was arranged relatively.Radix Aconiti Lateralis Preparata is except that the 3h group, and all the other each time dosage group hearts rate all have obvious quickening at different time, with model group significance or utmost point significant difference are arranged relatively.Through regression analysis, 2.5min, 5min, 10min, 15min optimal conditions be respectively 0.25 hour 25.06 times, 1.929 hours 25.82 times, 1.561 hours 29.39 times, 6 hours 25 times, 20min, 25min, 30min and 60min then are respectively 48 times of 6 hours 25.35 times, 0.25 hour 37.38 times, 6 hours 48 times and 6 hours.
The result sees table 1 and regression analysis for details.
The influence of table 1 pair acute heart failure yang deficiency rat heart rate (%)
Group |
Animal (only) |
Dosage (g/kg) |
After the administration |
2.5min |
5min |
10min |
The positive group of blank group model group |
10 10 10 |
-distilled water 24 |
98.1±12.2 34.6±8.4△△△ 37.9±18.2 |
97.3±7.9 48.8±11.9△△△ 61.3±10.4* |
99.5±7.1 54.8±5.87△△△ 64.7±12.6* |
Radix Aconiti Lateralis Preparata |
15min 30min 1h 2h 3h 4h 6h |
10 10 10 10 10 10 10 |
6 0.75 12 3 0.25 9 1.5 |
54.4±18.9** 28.7±10.6 32.2±21.4 46.5±11.8* 33.2±11.5 28.9±9.5 25.6±10.9 |
64.9±16.8* 45.1±5.8 41.9±16.7 67.4±19.7* 42.1±4.6 48.9±14.1 38.1±12.8 |
66.8±10.3** 59.3±16.4 61.9±9.6 71.3±22.8* 53.8±8.4 69.0±19.4* 54.8±11.3 |
After the administration |
15min |
20min |
25min |
30min |
60min |
104.7±11.8 61.1±7.1△△△ 70.5±17.5 75.5±7.7*** 67.3±15.6 72.7±9.6** 78.8±20.7* 60.6±10.1 73.3±14.8* 69.9±12.4 |
107.5±12.1 66.4±11.4△△△ 77.2±14.7 85.3±12.4** 71.1±14.7 79.7±12.1* 85.0±22.3* 67.7±15.9 82.9±17.5* 80.2±7.0** |
108.2±10.2 68.9±12.5△△△ 84.6±11.8** 92.8±13.1** 74.3±13.6 85.3±7.6** 85.1±22.3* 71.4±16.9 88.3±18.9* 82.4±3.6** |
110.1±13.7 70.4±9.7△△△ 88.6±12.6** 95.8±13.2*** 78.3±14.1 87.6±6.1*** 84.2±26.6 74.3±19.0 88.53±16.6** 84.17±3.16*** |
106.1±6.5 71.6±12.0△△△ 94.9±15.0** 98.0±13.1*** 89.2±20.0* 89.4±13.2** 79.1±12.6 76.1±15.0 97.1±7.2*** 84.4±12.1* |
Annotate: compare △ △ △ P<0.001 with the blank group; Compare * P<0.05, * * P<0.01, * * * P<0.001 with model group.
Regression analysis:
2.5min: optimal conditions: X1=0.25h, X2=25.06 doubly expect result of the test: Y=3.41
5min: optimal conditions: X1=1.929h, X2=25.82 doubly expect result of the test: Y=3.82
10min: optimal conditions: X1=1.561h, X2=29.39 doubly expect result of the test: Y=3.50
15min: optimal conditions: X1=6h, X2=26 doubly expect result of the test: Y=9.49
20min: optimal conditions: X1=6h, X2=25.35 doubly expect result of the test: Y=8.17
25min: optimal conditions: X1=0.25h, X2=37.38 doubly expect result of the test: Y=4.36
30min: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=7.24
60min: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=27.6
3.1.1.2 influence to the maximum climbing speed of left indoor pressure
After the model group rat gives propafenone 10.5mg/kg modeling, the maximum climbing speed of left indoor pressure (+dp/dtmax) significantly descend immediately, reduce to minimumly in the several seconds, slowly rise thereafter, continue tens of minutes, with the blank group utmost point significant difference is arranged relatively.Each time dosage group+dp/dtmax of Radix Aconiti Lateralis Preparata all has rising, and wherein 15min group, 1h group, 2h group and 4h group significantly rise, and with model group significant difference or utmost point significant difference are arranged relatively.Through regression analysis, 5min, 10min, 15min optimal conditions be respectively 0.25 hour 28.17 times, 4.551 hours 20.65 times, 2.654 hours 48 times, 20min, 25min, 30min and 60min then are respectively 48 times of 6 hours 34.75 times, 6 hours 48 times, 6 hours 45.09 times and 6 hours.The result sees table 2 and regression analysis for details.
The influence of table 2 couple acute heart failure yang deficiency rat+dp/dtmax (%)
Group |
Animal (only) |
Dosage (g/kg) |
After the administration |
2.5min |
5min |
10min |
The positive group of blank group model group |
10 10 10 |
-distilled water 24 |
97.5±5.1 41.9±12.5△△△ 87.9±22.3*** |
98.6±9.3 48.2±17.4△△△ 97.7±23.8*** |
101.2±10.9 55.4±11.2△△△ 131.9±60.8** |
Radix Aconiti Lateralis Preparata |
15min 30min 1h 2h 3h 4h 6h |
10 10 10 10 10 10 10 |
6 0.75 12 3 0.25 9 1.5 |
51.3±14.1 35.8±13.4 35.0±17.0 54.1±18.9 44.9±9.8 37.4±19.0 37.0±14.0 |
62.2±8.7* 43.8±12.9 46.1±21.7 61.9±21.5 48.9±11.8 48.3±23.4 41.5±20.3 |
67.8±9.2* 62.8±14.8 65.4±20.1 74.5±13.4** 54.3±14.2 66.4±15.3 53.0±15.7 |
After the administration |
15min |
20min |
25min |
30min |
60min |
109.2±13.4 65.7±4.5△△△ 145.4±73.8** 75.6±16.0 66.7±18.9 80.8±13.6** 78.3±10.2** 59.1±19.8 72.2±13.5 64.8±14.8 |
114.0±13.4 70.5±7.3△△△ 149.6±81.9** 82.1±16.5* 70.5±7.3 85.8±21.1* 77.7±14.3 62.2±20.8 81.5±11.5* 76.9±16.3 |
110.6±15.2 71.8±11.9△△△ 164.1±68.7*** 85.9±15.6* 72.2±14.4 85.4±18.6 72.4±11.9 68.1±19.0 81.7±11.8 80.8±14.4 |
107.1±16.1 67.2±15.0△△△ 162.4±62.5*** 80.4±12.1* 72.5±14.6 83.4±19.3* 72.6±14.6 65.6±16.7 81.9±13.7* 78.1±9.9 |
96.8±22.8 58.2±10.0△△△ 142.0±45.9*** 42.8±36.9 68.8±16.9 68.2±24.0 56.4±28.6 64.0±19.1 77.7±15.3** 64.1±10.9 |
Annotate: compare △ △ △ P<0.001: compare * P<0.05, * * P<0.01, * * * P<0.001 with model group with the blank group.
Regression analysis:
5min: optimal conditions: X1=0.25h, X2=28.17 doubly expect result of the test: Y=2.47
10min: optimal conditions: X1=4.551h, X2=20.65 doubly expect result of the test: Y=5.71
15min: optimal conditions: X1=2.654h, X2=48 doubly expect result of the test: Y=3.91
20min: optimal conditions: X1=6h, X2=34.75 doubly expect result of the test: Y=2.80
25min: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=12.4
30min: optimal conditions: X1=6h, X2=45.09 doubly expect result of the test: Y=3.92
60min: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=13.2
3.1.1.3 influence to the maximum fall off rate of left indoor pressure
After the model group rat gave propafenone 10.5mg/kg modeling, the maximum fall off rate of left indoor pressure (dp/dtmax) significantly raise immediately, rises to the highlyest in the several seconds, slowly descend thereafter, continue tens of minutes, with the blank group utmost point significant difference is arranged relatively.Each time dosage group-dp/dtmax of Radix Aconiti Lateralis Preparata all has decline, and wherein 15min group, 2h group and 4h group significantly descend, and-dp/dtmax percentage rate significantly rises, and with model group significant difference is arranged relatively.Through regression analysis, 5min, 10min, 15min optimal conditions be respectively 0.25 hour 29.58 times, 0.25 hour 27.41 times, 1.964 hours 30.7 times, 20min, 25min, 30min and 60min then are respectively 48 times of 6 hours 34.28 times, 6 hours 48 times, 4.827 hours 1 times and 6 hours.The result sees table 3 and regression analysis for details.
The influence of table 3 couple acute heart failure yang deficiency rat-dp/dtmax (%)
Group |
Animal (only) |
Dosage (g/kg) |
After the administration (%) |
2.5min |
5min |
10min |
The positive group of blank group model group |
10 10 10 |
-distilled water 24 |
104.0±5.4 60.5±14.01△△△ 81.4±22.8* |
99.7±6.3 67.0±15.4△△△ 104.6±19.4*** |
97.5±11.1 71.3±14.4△△△ 100.5±18.5*** |
Radix Aconiti Lateralis Preparata |
15min 30min 1h 2h 3h 4h 6h |
10 10 10 10 10 10 10 |
6 0.75 12 3 0.25 9 1.5 |
67.1±6.6 49.8±20.5 52.8±23.6 69.5±25.2 54.6±19.8 53.8±22.8 50.0±26.0 |
80.8±9.7* 55.3±18.0 65.2±27.4 77.9±24.7 60.0±18.0 64.1±22.0 57.2±30.3 |
89.2±21.5* 77.3±18.5 84.0±25.8 88.7±19.8* 69.3±12.5 82.0±12.0 69.5±20.8 |
After the administration |
15min |
20min |
25min |
30min |
60min |
100.7±14.3 77.5±7.6△△△ 101.8±13.9*** 95.4±22.7* 79.7±19.6 93.2±27.2 89.5±21.4 75.3±15.1 87.8±11.4* 78.0±22.1 |
108.2±9.5 79.2±12.7△△△ 104.8±19.3** 96.9±22.7* 82.4±21.0 95.9±30.5 88.6±31.2 82.6±16.9 90.0±7.2* 91.6±21.5 |
98.3±10.7 80.7±8.4△△△ 105.9±22.4** 97.9±20.2* 84.1±21.7 91.0±22.9 80.9±36.4 82.2±17.6 87.4±4.8* 90.6±21.6 |
91.8±10.3 71.7±11.6△△ 105.2±17.2*** 85.1±23.0 77.1±19.4 81.2±23.1 78.9±34.9 83.2±17.9 82.4±8.2* 82.2±15.2 |
80.8±7.6 59.6±19.5△△ 87.9±16.2** 39.8±36.0 59.6±16.4 65.6±22.4 50.7±28.6 66.1±15.2 64.8±11.8 61.3±15.2 |
Annotate: compare △ △ P<0.01, △ △ △ P<0.001 with the blank group; Compare * P<0.05, * * P<0.01, * * * P<0.001 with model group.
Regression analysis:
5min: optimal conditions: X1=0.25h, X2=29.58 doubly expect result of the test: Y=2.58
10min: optimal conditions: X1=0.25h, X2=27.41 doubly expect result of the test: Y=2.58
15min: optimal conditions: X1=1.964h, X2=30.7 doubly expect result of the test: Y=2.67
20min: optimal conditions: X1=6h, X2=34.28 doubly expect result of the test: Y=2.94
25min: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=16.6
30min: optimal conditions: X1=4.827h, X2=1 doubly expect result of the test: Y=3.39
60min: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=3.6
3.1.2 insufficiency of kidney-YANG test
3.1.2.1 Radix Aconiti Lateralis insufficiency of kidney-YANG test
3.1.2.1.1 influence to insufficiency of kidney-YANG rat general state
The model group animal give the modeling medicine after 375mg/kg.d-117 days general state relatively poor, body weight significantly alleviates, and is fluffy by hair, drowsiness is moving less, urine is increased, aversion to cold and preference for warmth, food ration reduces.It is better that Radix Aconiti Lateralis 15min group, 1h group and 6h organize general situation, the trend that body weight is significantly increased, especially with the 6h group for very, modeling in continuous 17 days, not seeing has animal dead.The result sees table 4 for details.
The influence of table 4 pair insufficiency of kidney-YANG rat body weight
Group |
Animal (only) |
Dosage (g/kg) |
Body weight (g) |
Before the administration |
5 days |
The positive group of blank group model group |
10 9 10 |
-distilled water 3.33 |
215.60±13.01 223.33±20.47 219.00±15.08 |
234.00±15.00 228.54±21.98 221.42±22.07 |
Radix Aconiti Lateralis |
15min 30min 1h 2h 3h 4h 6h |
8 8 9 9 8 6 12 |
6 0.75 12 3 0.25 9 1.5 |
223.00±20.19 223.17±24.69 221.67±20.79 218.67±17.76 221.58±21.56 218.67±15.19 219.42±17.97 |
230.33±25.49 228.58±25.48 227.25±20.75 227.58±24.41 229.50±22.37 218.27±18.22 232.00±16.37 |
|
Body weight (g) |
10 days |
13 days |
17 days |
249.30±15.35 229.67±34.46 226.73±26.41 229.92±40.64 216.00±35.51 226.18±14.65 227.70±29.57 213.08±31.31 209.80±16.21 230.67±20.16 |
259.50±18.18 236.33±22.38△ 215.18±37.26 232.30±41.22 219.60±27.77 228.20±19.76 226.80±39.07 217.36±24.81 213.38±25.67 227.25±20.18 |
264.10±16.82 220.89±21.93△△△ 221.80±28.25 243.86±34.00 220.63±17.74 225.78±24.21 229.11±34.62 221.88±26.24 205.00±21.61 232.83±24.07 |
Annotate: compare △ P<0.05, △ △ △ P<0.001 with the blank group.
3.1.2.1.2 influence to the insufficiency of kidney-YANG rat temperature
3.1.2.1.2.1 16 days body temperature of insufficiency of kidney-YANG rat modeling changes
The 10th day body temperature of model group rat modeling promptly significantly reduces, and with the blank group significant difference is arranged relatively.After giving the Radix Aconiti Lateralis medicinal liquid, temperature significantly raises, and organizes to very with 1h group and 6h especially, with model group significance or utmost point significant difference is arranged relatively.Regression analysis shows, and is remarkable with 6 hours 48 times of thermogenic actions.The result sees table 5 and regression analysis for details.
The influence of table 5 pair insufficiency of kidney-YANG rat temperature
Group |
Animal (only) |
Dosage (g/kg) |
Body temperature (℃) |
Before the administration |
5 days |
The positive group of blank group model group |
10 9 10 |
-distilled water 3.33 |
37.39±0.62 37.40±0.44 37.37±0.34 |
36.71±0.42 36.81±0.42 37.13±0.70 |
Radix Aconiti Lateralis |
15min 30min 1h 2h 3h 4h 6h |
8 8 9 9 8 6 12 |
6 0.75 12 3 0.25 9 1.5 |
37.38±0.41 37.58±0.26 37.58±0.60 37.56±0.33 37.45±0.66 37.42±0.65 37.44±0.35 |
36.77±0.36 37.05±0.38 37.04±0.23 37.05±0.27 36.66±0.55 36.88±0.30 37.18±0.42* |
Body temperature (℃) |
10 days |
13 days |
16 days |
37.05±0.36 36.34±0.68△△ 36.35±0.35 36.14±0.52 36.49±0.89 37.20±0.67** 36.71±0.38 36.72±0.85 36.24±0.89 37.27±0.53** |
36.87±0.29 36.83±0.47 36.54±0.88 37.16±0.66 37.03±0.38 37.42±0.46* 36.90±0.51 36.65±1.36 37.30±0.33* 36.84±0.28 |
37.14±0.51 36.92±0.66 37.35±0.83 36.83±0.37 36.69±0.52 36.97±0.61 36.96±0.51 36.84±0.47 36.47±1.09 37.61±0.70* |
Annotate: compare △ △ P<0.01 with the blank group; Compare * P<0.05, * * P<0.01 with model group.
Regression analysis:
The 5th day: optimal conditions: X1=6h, X2=48 doubly expected result of the test: Y=9.83
The 10th day: optimal conditions: X1=6h, X2=1 doubly expected result of the test: Y=6.53
The 13rd day: optimal conditions: X1=6h, X2=48 doubly expected result of the test: Y=7.19
The 16th day: optimal conditions: X1=6h, X2=10.49 doubly expected result of the test: Y=2.37
3.1.2.1.2.2 influence to 12 division of day and night of insufficiency of kidney-YANG rat body temperature
The modeling of model group rat after 16 days body temperature generally reduce, give the Radix Aconiti Lateralis medicinal liquid after body temperature obviously raise, group significantly raise at 9 o'clock-21 o'clock body temperature in 6 hours, with model group significance or utmost point significant difference was arranged relatively.The result sees table 6 for details.
The influence of table 6 pair 12 division of day and night of insufficiency of kidney-YANG rat body temperature
Group |
Animal (only) |
Dosage (g/kg) |
Body temperature (℃) |
During 9-11 during 11-13 during 13-15 |
The positive group of blank group model group |
10 9 10 |
-distilled water 3.33 |
37.14±0.51 37.22±0.24 37.22±0.27 36.92±0.66 37.16±0.59 37.07±0.65 37.35±0.83 37.18±0.61 37.33±0.57 |
Radix Aconiti Lateralis |
15min 30min 1h 2h 3h 4h 6h |
8 8 9 9 8 6 12 |
6 0.75 12 3 0.25 9 1.5 |
36.83±0.37 37.14±0.48 37.05±0.44 36.69±0.52 36.95±0.73 37.06±0.80 36.97±0.61 37.08±0.55 37.11±0.53 36.96±0.51 36.98±0.65 36.98±0.59 36.84±0.47 36.88±0.30 37.00±0.50 36.47±1.09 36.62±1.15 36.83±0.48 37.61±0.70* 37.99±0.82** 37.83±0.79** |
Body temperature (℃) |
During 15-17 |
During 17-19 |
During 19-21 |
During 21-23 |
During 23-1 |
36.60±0.42 36.58±0.87 36.81±0.69 36.76±0.43 36.91±0.94 36.67±0.81 37.22±0.55 36.93±0.40 36.92±0.57 37.83±0.82*** |
37.15 ±0.32 37.12 ±0.83 37.14±0.81 37.04±0.48 37.06±0.49 36.90±0.66 37.37±0.60 37.08±0.51 37.10±0.39 37.71±0.73* |
37.19±0.21 37.02±0.56 37.24±0.49 36.98±0.51 37.10±0.58 37.11±0.48 37.44±0.51 37.09±0.39 37.23±0.70 37.58±0.86* |
37.20±0.21 37.41±0.67 37.41±0.49 37.14±0.53 37.18±0.76 37.72±0.43 37.58±0.35 37.41±0.30 37.650.59 37.87±0.71 |
37.32±0.25 37.68±0.46 37.59±0.61 37.20±0.48 37.28±0.52 37.39±0.41 37.46±0.53 37.18±0.69 37.15±0.99 37.35±0.88 |
Body temperature (℃) |
During 1-3 |
During 3-5 |
During 5-7 |
During 7-9 |
37.87±0.32 37.77±0.52 37.60±0.45 37.26±0.57 37.39±0.70 37.43±0.50 37.51±0.39 37.11±0.89 37.70±0.68 37.85±0.64 |
38.11±0.23 37.53±0.44△ 37.80±0.60 37.30±0.81 37.23±0.47 37.37±0.58 37.51±0.48 37.08±0.35 37.32±1.04 37.48±0.81 |
37.78±0.18 37.69±0.56 37.39±0.70 37.11±0.97 37.30±0.82 36.83±1.76 37.20±0.45 37.25±0.52 37.40±1.09 37.29±1.01 |
37.05±0.40 37.41±0.57 37.37±0.62 36.88±1.10 36.88±0.83 37.06±0.72 37.24±0.65 37.19±0.44 37.33±1.02 37.43±0.77 |
Annotate: compare △ P<0.05 with the blank group; Compare * P<0.05, * * P<0.01, * * * P<0.001 with model group.
3.1.2.1.3 influence to the insufficiency of kidney-YANG rats'swimming time
The model group rats'swimming time significantly shortens, and with the blank group utmost point significant difference is arranged relatively.Radix Aconiti Lateralis 15min group, 1h group and 6h group swimming time significant prolongation compare with model group, and utmost point significant difference is arranged.Through regression analysis, the Best Times dosage of Radix Aconiti Lateralis performance drug effect be 6 hours 48 times.The result sees table 7 and regression analysis for details.
The influence of table 7 pair yang deficiency rats'swimming time
Group |
Animal (only) |
Dosage (g/kg) |
Swimming time (second) |
The positive group of blank group model group |
10 9 10 |
-distilled water 3.33 |
258.30±59.38 152.67±68.86△△ 259.90±50.93** |
Radix Aconiti Lateralis |
15min 30min 1h 2h 3h 4h 6h |
8 8 9 9 8 6 12 |
6 0.75 12 3 0.25 9 1.5 |
271.63±23.93*** 194.00±60.95 258.00±62.30** 159.33±36.08 211.50±72.12 214.00±66.15 262.08±83.02** |
Annotate: compare △ △ P<0.01 with the blank group; Compare * * P<0.01, * * * P<0.001 with model group.
Regression analysis:
Optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=18.8
3.1.2.1.4 influence to insufficiency of kidney-YANG Rats Organs and Tissues coefficient
The modeling of model group rat is the remarkable atrophy of thymus after 17 days, and spleen obviously reduces.1h group, 2h group, 3h organize, 4h organizes and 6h group thymus obviously increases, and 1h group and 4h group spleen obviously increase weight, and compare with model group, and significance or utmost point significant difference are arranged.The result sees table 8 and regression analysis for details.
The influence of table 8 pair insufficiency of kidney-YANG Rats Organs and Tissues coefficient (%)
Group |
Animal (only) |
Dosage (g/kg) |
Thymus |
Spleen |
The positive group of blank group model group |
10 9 10 |
-distilled water 3.33 |
0.1156±0.0412 0.0257±0.0118△△△ 0.0521±0.0186** |
0.482±0.128 0.395±0.060 0.486±0.204 |
Radix Aconiti Lateralis |
15min 30min 1h 2h 3h 4h 6h |
8 8 9 9 8 6 12 |
6 0.75 12 3 0.25 9 1.5 |
0.0393±0.0147 0.0402±0.0225 0.0465±0.0183* 0.0536±0.0286* 0.0478±0.0199* 0.0657±0.0221*** 0.0544±0.0108*** |
0.583±0.304 0.508±0.157 0.525±0.148* 0.453±0.172 0.553±0.107 0.722±0.327* 0.524±0.191 |
Annotate: compare △ △ △ P<0.001 with the blank group; Compare * P<0.05, * * P<0.01, * * * P<0.001 with model group.
Regression analysis:
Thymus: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=6.81
Spleen: optimal conditions: X1=5.23h, X2=48 doubly expect result of the test: Y=3.82
3.1.2.2 Radix Aconiti Lateralis Preparata insufficiency of kidney-YANG test
3.1.2.2.1 swimming test
3.1.2.2.1.1 general state
The model group animal give continuously the modeling medicine after 40mg/kg.d-110 days general state relatively poor, body weight does not increase, and is fluffy by hair, drowsiness is moving less, the urine showed increased, aversion to cold and preference for warmth, food ration obviously reduces.It is better that Radix Aconiti Lateralis Preparata 15min organizes, 6h organizes general situation, and body weight increases gradually.The last body weight, Radix Aconiti Lateralis Preparata 6h group relatively has significant difference with model group.Regression analysis is the result show, the best decocting time of Radix Aconiti Lateralis Preparata weight increase and dosage were respectively 6 hours, 19.89 times.The result sees table 9 and regression analysis for details.
The influence of table 9 pair insufficiency of kidney-YANG mice body weight
Group |
Animal (only) |
Dosage (g/kg) |
Body weight (g) |
Before the medicine |
The 5th day |
The 10th day |
The positive group of blank group model group |
11 14 14 |
-distilled water 3.33 |
25.68±2.53 25.52±2.34 25.32±2.20 |
29.84±3.39 25.02±3.74△△ 26.22±2.30 |
32.54±3.17 24.87±4.19△△△ 26.36±2.50 |
Radix Aconiti Lateralis Preparata |
15min 30min 1h 2h 3h 4h 6h |
14 12 15 15 13 15 12 |
6 0.75 12 3 0.25 9 1.5 |
25.84±1.82 25.06±2.87 25.74±1.83 25.87±1.52 26.38±1.94 25.58±2.17 26.16±1.73 |
26.74±1.96 26.84±2.83 26.31±2.76 26.02±2.54 27.39±2.60 25.98±3.41 26.89±3.24 |
27.30±2.10 26.98±3.54 26.46±2.72 26.31±2.24 25.79±2.99 26.25±3.01 27.68±2.62* |
Annotate: compare △ △ P<0.01, △ △ △ P<0.001 with the blank group; Compare * P<0.05 with model group.
Regression analysis:
Body weight: optimal conditions: X1=6h, X2=19.89 doubly expect result of the test: Y=2.18
3.1.2.2.1.2 influence to swimming time
Blank group low temperature swimming time average out to 223.73 seconds, model group mice swimming time significantly shortens, and average swimming time only is 102.86 seconds, with the blank group utmost point significant difference is arranged relatively.Radix Aconiti Lateralis Preparata is except that the 3h group, and all the other each administration group swimming times all obviously prolong, and with model group significance or utmost point significant difference are arranged relatively.Regression analysis shows that the Best Times dosage that Radix Aconiti Lateralis Preparata prolongs insufficiency of kidney-YANG mice swimming time is 6h23.18 times.The result sees table 10 and regression analysis for details.
The influence of table 10 pair insufficiency of kidney-YANG mice swimming time
Group |
Animal (only) |
Dosage (g/kg) |
Swimming time (second) |
The positive group of blank group model group |
11 14 14 |
-distilled water 3.33 |
223.73±95.01 102.86±35.49△△△ 181.29±65.64*** |
Radix Aconiti Lateralis Preparata |
15min 30min 1h 2h 3h 4h 6h |
14 12 15 15 13 15 12 |
6 0.75 12 3 0.25 9 1.5 |
180.64±65.07*** 145.67±56.20* 163.25±55.41** 166.67±65.97** 145.62±77.18 170.20±86.40* 179.58±56.22*** |
Annotate: compare △ △ △ P<0.001 with the blank group; Compare * P<0.05, * * P<0.01, * * * P<0.001 with model group.
Regression analysis:
Swimming time: optimal conditions: X1=6h, X2=23.18 doubly expect result of the test: Y=7.01
3.1.2.2.2 pole-jump test
The pole-climbing of blank group mice is agile, and the average pole-climbing time reaches 845.18 seconds; Model group animal pole-climbing bradykinesia, relatively has utmost point significant difference with the blank group at the average only 268.64 seconds pole-climbing time.Each administration group pole-climbing time of Radix Aconiti Lateralis Preparata all prolongs, and is wherein obvious with 15min group, 1h group and 6h group, with model group significance or utmost point significant difference arranged relatively.Through regression analysis, the Best Times dosage that Radix Aconiti Lateralis Preparata prolongs the insufficiency of kidney-YANG mice pole-climbing time is respectively 6h14.54 doubly.The result sees table 11 and regression analysis for details.
The influence of table 11 pair insufficiency of kidney-YANG mice pole-climbing time
Group |
Animal (only) |
Dosage (g/kg) |
The pole-climbing time (second) |
The positive group of blank group model group |
11 11 13 |
-distilled water 3.33 |
845.18±108.69 268.64±169.01△△△ 528.92±250.54** |
Radix Aconiti Lateralis Preparata |
15min 30min 1h 2h 3h 4h 6h |
13 10 15 13 13 13 12 |
6 0.75 12 3 0.25 9 1.5 |
650.69±270.16*** 447.704±331.78 506.73±306.16* 497.46±344.45 351.92±272.98 415.54±290.24 712.83±245.24*** |
Annotate: compare △ △ △ P<0.001 with the blank group; Compare * P<0.05, * * P<0.01, * * * P<0.001 with model group.
Regression analysis:
Pole-climbing time: optimal conditions: X1=6h, X2=14.54 doubly expect result of the test: Y=5.45
3.1.2.3 RADIX ACONITI LATERALIS PREPARATA insufficiency of kidney-YANG test
3.1.2.3.1 swimming test
3.1.2.3.1.1 general state
The model group animal gives the modeling medicine after 40mg/kg.d-110 days continuously, and general state is relatively poor, and body weight does not increase, and is fluffy by hair, and drowsiness is moving less, the urine showed increased, and aversion to cold and preference for warmth, food ration obviously reduces.It is better that RADIX ACONITI LATERALIS PREPARATA 15min group, 30min group, 4h group and 6h organize general situation, and body weight increases gradually.The last body weight, RADIX ACONITI LATERALIS PREPARATA 30min group and 4h group relatively have significant difference with model group.Regression analysis is the result show, the best decocting time of RADIX ACONITI LATERALIS PREPARATA weight increase and dosage were respectively 6 hours, 48 times.The result sees table 12 and regression analysis for details.
The influence of table 12 pair insufficiency of kidney-YANG mice body weight
Group |
Animal (only) |
Dosage (g/kg) |
Body weight (g) |
Before the medicine |
The 5th day |
The 10th day |
The positive group of blank group model group |
10 9 10 |
-distilled water 3.33 |
20.93±1.14 20.96±1.41 20.67±1.71 |
26.08±1.37 20.52±2.04△△△ 21.43±1.93 |
28.63±2.68 20.90±2.19△△△ 21.61±1.95 |
RADIX ACONITI LATERALIS PREPARATA |
15min 30min 1h 2h 3h 4h 6h |
12 9 10 9 9 8 10 |
6 0.75 12 3 0.25 9 1.5 |
21.06±1.45 21.03±1.44 20.82±1.28 21.08±1.44 21.27±1.35 20.57±1.56 20.96±1.29 |
22.78±1.55** 22.10±2.01 21.85±2.48 21.38±1.70 21.61±2.19 20.90±2.17 22.45±2.27* |
22.57±2.20 23.56±2.34* 22.06±3.17 21.14±2.17 21.79±3.59 23.53±2.48* 22.25±2.05 |
Annotate: compare △ △ △ P<0.001 with the blank group; Compare * P<0.05, * * P<0.01 with model group.
Regression analysis:
Body weight (last): optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=9.76
3.1.2.3.1.2 influence to swimming time
Blank group low temperature swimming time average out to 232.5 seconds, model group mice swimming time significantly shortens, and on average only 129.67 seconds, with the blank group utmost point significant difference is arranged relatively.RADIX ACONITI LATERALIS PREPARATA 1h group, 4h group and 6h group swimming time significant prolongation relatively have significance or utmost point significant difference with model group.All the other each administration group swimming times have the trend of obvious prolongation.Regression analysis shows that the Best Times dosage that RADIX ACONITI LATERALIS PREPARATA prolongs insufficiency of kidney-YANG mice swimming time is 6h, 37.57 times.The result sees table 13 and regression analysis for details.
The influence of table 13 pair insufficiency of kidney-YANG mice swimming time
Group |
Animal (only) |
Dosage (g/kg) |
Swimming time (second) |
The positive group of blank group model group |
10 9 10 |
-distilled water 3.33 |
232.50±41.24 129.67±44.68△△△ 193.09±44.98** |
RADIX ACONITI LATERALIS PREPARATA |
15min 30min 1h 2h 3h 4h 6h |
12 9 10 9 9 8 10 |
6 0.75 12 3 0.25 9 1.5 |
182.17±66.45 141.11±72.73 195.64±85.22* 158.33±50.73 128.78±47.58 228.88±63.69** 177.36±46.47* |
Annotate: compare △ △ △ P<0.001 with the blank group; Compare * P<0.05, * * P<0.01 with model group.
Regression analysis:
Swimming time: optimal conditions: X1=6h, X2=37.57 doubly expect result of the test: Y=9.23
3.1.2.3.2 pole-jump test
The pole-climbing of blank group mice is agile, and the average pole-climbing time is 547.6 seconds; Model group animal pole-climbing bradykinesia, relatively has utmost point significant difference with the blank group at the average only 142 seconds pole-climbing time.Each administration group pole-climbing time of RADIX ACONITI LATERALIS PREPARATA all prolongs, and is wherein obvious with 15min group and 1h group, with model group significant difference arranged relatively.Through regression analysis, the Best Times dosage that RADIX ACONITI LATERALIS PREPARATA prolongs the insufficiency of kidney-YANG mice pole-climbing time is respectively 6h23.94 doubly.The result sees table 14 and regression analysis for details.
The influence of table 14 pair insufficiency of kidney-YANG mice pole-climbing time
Group |
Animal (only) |
Dosage (g/kg) |
The pole-climbing time (second) |
The positive group of blank group model group |
10 9 10 |
-distilled water 3.33 |
547.60±275.09 142.00±97.08△△△ 352.60±224.36* |
RADIX ACONITI LATERALIS PREPARATA |
15min 30min 1h 2h 3h 4h 6h |
11 9 10 8 9 8 9 |
6 0.75 12 3 0.25 9 1.5 |
357.00±257.14* 153.56±101.60 294.70±178.59* 235.38±280.55 126.44±138.98 210.13±131.90 261.11±158.88 |
Annotate: compare △ △ △ P<0.001 with the blank group; Compare * P<0.05 with model group.
Regression analysis:
Pole-climbing time: optimal conditions: X1=6h, X2=23.94 doubly expect result of the test: Y=4.59
3.1.3 yang deficiency constipation test
3.1.3.1 general situation
The model group animal just occurred the abdominal distention state on the 2nd day in modeling individually, generally asthenia occurred in the time of modeling 3-4 days and rolled up, the movable minimizing, fur is fluffy, later stage animal dead rate is higher, all because of abdominal distention greatly till death, animal is thin and weak, the feels fur that touches relaxes, no meat.The 1h of positive drug group, Radix Aconiti Lateralis, Radix Aconiti Lateralis Preparata, RADIX ACONITI LATERALIS PREPARATA group and 6h treated animal general state are better, and activity is more frequent, and fur is slightly fluffy, but touch voluptuousness arranged.With model group significant difference is arranged relatively.Each organizes weight ratio, there was no significant difference.
3.1.3.2 defecation incubation period and defecation grain number
The modeling of model group mice is defecation significant prolongation incubation period after 9 days, and defecation grain digital display work reduced in 3 hours, compared with the blank group, and utmost point significant difference is arranged.Radix Aconiti Lateralis defecation incubation period all significantly shortens except that 15min group and 4h group, and the defecation grain was counted 1h group and the remarkable increase of 6h group in 3 hours; Radix Aconiti Lateralis Preparata defecation 60min incubation period group, 3h group, 4h group and 6h organize all and significantly shorten, and the defecation grain was counted 30min group and the significantly increase of 6h group in 3 hours; RADIX ACONITI LATERALIS PREPARATA defecation 30min incubation period group and 4h, 6h organize all and significantly shorten, and the defecation grain was counted 1h group and the significantly increase of 6h group in 3 hours.With model group significance or utmost point significant difference are arranged relatively.Through regression analysis, Radix Aconiti Lateralis defecation incubation period and defecation grain are counted optimal conditions and are respectively 48 times of 6 hours 19.71 times and 6 hours; Radix Aconiti Lateralis Preparata defecation incubation period and defecation grain are counted optimal conditions and are 6 hours 48 times; Radix Aconiti Lateralis Preparata defecation incubation period and defecation grain are counted optimal conditions and are 6 hours 48 times.The result sees table 15,16,17 and regression analysis for details.
Table 15 Radix Aconiti Lateralis is to the influence of yang deficiency mice with constipation defecation incubation period and granule number
Group |
Animal (only) |
Dosage (g/kg) |
Incubation period (branch) |
Granule number (grain) |
The positive group of blank group model group |
10 15 11 |
-distilled water 0.5 |
23.54±6.23 104.53±56.24△△△ 69.27±43.79 |
12.87±5.13 3.67±3.62△△△ 7.39±4.09* |
Radix Aconiti Lateralis |
15min 30min 1h 2h 3h 4h 6h |
16 15 8 12 14 14 12 |
6 0.75 12 3 0.25 9 1.5 |
80.25±43.19 60.87±27.06* 54.75±42.44* 52.75±49.28* 58.86±52.88* 57.93±74.15 50.17±46.58* |
4.44±3.83 5.00±3.64 8.88±3.87** 4.83±3.10 4.71±3.75 7.14±7.12 7.08±3.80* |
Annotate: compare △ △ △ P<0.001 with the blank group; Compare * P<0.05, * * P<0.01 with model group.
Regression analysis:
Incubation period: optimal conditions: X1=6h, X2=19.71 doubly expect result of the test: Y=4.34
Defecation frequency: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=4.62
Table 16 Radix Aconiti Lateralis Preparata is to the influence of yang deficiency mice with constipation defecation incubation period and defecation grain number
Group |
Animal (only) |
Dosage (g/kg) |
Defecation incubation period (branch) |
Defecation grain number (grain/3h) |
The positive group of blank group model group |
13 11 10 |
-distilled water 0.5 |
25.14±6.72 61.91±59.03△△ △ |
13.31±3.75 4.73±4.36△△△ 9.60±4.38* |
Radix Aconiti Lateralis Preparata |
15min 30min 1h 2h 3h 4h 6h |
10 10 10 10 10 11 12 |
6 0.75 12 3 0.25 9 1.5 |
20.00±9.99* 41.80±33.84 35.40±30.44 12.70±7.24* 23.00±10.63 17.60±10.92* 19.55±5.80* 9.08±7.11* |
7.10±3.64 5.80±4.49* 7.60±2.76 7.50±4.81 7.20±3.52 8.36±3.83 10.67±5.33** |
Annotate: compare △ △ △ P<0.001 with the blank group; Compare * P<0.05, * * P<0.01, * * * P<0.001 with model group.
Regression analysis:
Defecation incubation period:
Optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=4.03
The defecation granule number:
Optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=3.53
Table 17 RADIX ACONITI LATERALIS PREPARATA is to the influence of yang deficiency mice with constipation defecation incubation period and defecation grain number
Group |
Animal (only) |
Dosage (g/kg) |
Incubation period (branch) |
Granule number (grain) |
The positive group of blank group model group |
10 15 11 |
-distilled water 0.5 |
23.54±6.23 104.53±56.24△△△ 69.27±43.79 |
12.87±5.13 3.67±3.62△△△ 7.39±4.09* |
RADIX ACONITI LATERALIS PREPARATA |
15min 30min 1h 2h 3h 4h 6h |
13 13 11 12 14 14 10 |
6 0.75 12 3 0.25 9 1.5 |
81.38±39.88 71.15±8.91* 76.27±11.87 68.50±65.57 83.21±38.60 61.21±36.54* 59.00±15.62* |
4.54±3.58 5.31±3.43 7.18±3.74* 3.83±4.11 4.64±4.01 5.50±3.76 8.50±2.64** |
Annotate: compare △ △ △ P<0.001 with the blank group; Compare * P<0.05, * * P<0.01 with model group.
Regression analysis:
Incubation period: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=8.20
Defecation frequency: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=5.42
3.1.3.3 to the propulsive influence of gastrointestinal
Model group mice gastrointestinal propelling rate significantly reduces, and with the blank group utmost point significant difference is arranged relatively.Radix Aconiti Lateralis except that 15min group all the other respectively organize gastrointestinal propelling rate and significantly increase: Radix Aconiti Lateralis Preparata 1h group and 6h organize gastrointestinal propelling rate significant prolongation; RADIX ACONITI LATERALIS PREPARATA is except that 30min group and 1h group, and all the other respectively organize gastrointestinal propelling rate significantly increases.With model group significance or utmost point significant difference are arranged relatively.Through regression analysis, the optimal conditions of Radix Aconiti Lateralis performance drug effect be 6 hours 48 times; Radix Aconiti Lateralis Preparata be 6 hours 1 times; The optimal conditions of RADIX ACONITI LATERALIS PREPARATA performance drug effect be 6 hours 48 times.The result sees table 18,19,20 and regression analysis for details.
Table 18 Radix Aconiti Lateralis is to the propulsive influence of yang deficiency mice with constipation gastrointestinal
Group |
Animal (only) |
Dosage (g/kg) |
Gastrointestinal propelling rate (%) |
The positive group of blank group model group |
10 15 10 |
-distilled water 0.5 |
74±12 33±11△△△ 56±22** |
Radix Aconiti Lateralis |
15min 30min 1h 2h 3h 4h 6h |
13 15 8 12 12 12 12 |
6 0.75 12 3 0.25 9 1.5 |
32±9 43±13* 56±23** 48±21* 47±13** 55±19*** 47±12** |
Annotate: compare △ △ △ P<0.001 with the blank group; Compare * P<0.05, * * P<0.01, * * * P<0.001 with model group.
Regression analysis:
Gastrointestinal advances: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=9.62
Table 19 Radix Aconiti Lateralis Preparata is to the influence of yang deficiency mice with constipation gastrointestinal propelling rate
Group |
Animal (only) |
Dosage (g/kg) |
Gastrointestinal propelling rate (%) |
The positive group of blank group model group |
13 11 10 |
-distilled water 0.5 |
68.33±7.12 38.24±9.34△△△ 62.81±9.24*** |
Radix Aconiti Lateralis Preparata |
15min 30min 1h 2h 3h 4h 6h |
10 10 10 10 10 11 12 |
6 0.75 12 3 0.25 9 1.5 |
34.24±8.73 34.65±13.01 53.58±10.63** 36.65±12.64 46.51±11.61 36.11±8.14 53.61±6.29** |
Annotate: compare △ △ △ P<0.001 with the blank group; Compare * P<0.05, * * P<0.01, * * * P<0.001 with model group.
Regression analysis:
Regression equation: optimal conditions: X1=6h, X2=1 doubly expect result of the test: Y=5.38
Table 20 RADIX ACONITI LATERALIS PREPARATA is to the influence of yang deficiency mice with constipation gastrointestinal propelling rate
Group |
Animal (only) |
Dosage (g/kg) |
Gastrointestinal propelling rate (%) |
The positive group of blank group model group |
10 15 10 |
-distilled water 0.5 |
74±12 33±11△△△ 56±22 |
RADIX ACONITI LATERALIS PREPARATA |
15min 30min 1h 2h 3h 4h 6h |
13 11 10 10 12 13 10 |
6 0.75 12 3 0.25 9 1.5 |
53±18** 42±18 40±12 45±13* 50±14** 50±21* 58±15*** |
Annotate: compare △ △ △ P<0.001 with the blank group; Compare * P<0.05, * * P<0.01, * * * P<0.001 with model group.
Regression analysis:
Gastrointestinal advances: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=10.1
3.2 antiinflammatory test
3.2.1 dimethylbenzene antiinflammatory test
The 1.5h auricle edema is obvious after the modeling of model group mice.The Radix Aconiti Lateralis group is except that 2h, 3h, 4h, and all the other are respectively organized auricle edema and all obviously dwindle, and compares with model group, and significance or utmost point significant difference are all arranged.Through regression analysis, the optimal conditions of Radix Aconiti Lateralis performance drug effect is 0.25h, 48 times.The result sees table 21 and regression analysis for details.
Table 21 xylol causes the influence of scorching mice auricle swelling
Group |
Animal (only) |
Dosage (g/kg) |
Auricle swelling degree (mg) |
The positive group of model group |
10 10 |
Distilled water 0.003 |
10.80±2.30 6.40±2.46*** |
Radix Aconiti Lateralis |
15min 30min 1h 2h 3h 4h 6h |
10 10 10 10 10 10 10 |
6 0.75 12 3 0.25 9 1.5 |
8.00±1.56** 8.20±3.12* 7.60±2.17** 10.90±0.88 9.40±1.35 10.20±2.35 8.50±2.55* |
Annotate: compare * P<0.05, * *<P0.01, * * * P<0.001 with model group.
Regression analysis:
Optimal conditions: X1=0.25h, X2=48 doubly expect result of the test: Y=5.2
3.2.2 influence to the Ovum Gallus domesticus album pedal swelling
Very fast swelling behind the right sufficient sole of the foot injection Ovum Gallus domesticus album of model group rat, the 1h swelling of injection back reaches the peak, and still swelling is obvious for injection back 6h.The Radix Aconiti Lateralis group is except that multiple dose was invalid in 3 hours 1, and all the other all have the effect of obvious inhibition rat paw edema.Through regression analysis, the optimal conditions of Radix Aconiti Lateralis performance drug effect: when 30min, 1h, 2h, 6h 6h, 48 times; When 3h, 4h 0.25h, 31.64 times and 31.93 times.The result sees table 22 and regression analysis for details.
Table 22 pair Ovum Gallus domesticus album causes the influence of scorching rat paw edema
Group |
Animal (only) |
Dosage (g/kg) |
Swelling degree (ml) |
0.5h |
1h |
2h |
The positive group of model group |
10 10 |
Distilled water 0.003 |
0.67±0.25 0.58±0.22 |
0.86±0.26 0.66±0.26 |
0.74±0.23 0.52±0.21* |
Radix Aconiti Lateralis |
15min 30min 1h 2h 3h 4h 6h |
10 10 10 10 10 10 10 |
6 0.75 12 3 0.25 9 1.5 |
0.32±0.14** 0.61±0.31 0.49±0.17 0.59±0.27 0.71±0.24 0.51±0.30 0.50±0.22 |
0.28±0.18*** 0.65±0.35 0.49±0.17** 0.64±0.35 0.78±0.20 0.59±0.22* 0.50±0.25 |
0.32±0.15*** 0.56±0.36 0.47±0.23* 0.50±0.29* 0.64±0.22 0.52±0.24* 0.46±0.21* |
|
Swelling degree (ml) |
3h |
4h |
6h |
0.69±0.20 0.38±0.19** 0.25±0.17*** 0.41±0.26* 0.39±0.22** 0.41±0.29* 0.57±0.17 0.46±0.22* 0.33±0.16*** |
0.56±0.19 0.20±0.13*** 0.18±0.18*** 0.39±0.22 0.37±0.22* 0.37±0.25* 0.52±0.19 0.48±0.18 0.22±0.13*** |
0.46±0.13 0.07±0.10*** 0.15±0.11*** 0.28±0.24 0.27±0.18* 0.28±0.25* 0.34±0.17 0.28±0.14** 0.18±0.13*** |
Annotate: compare * P<0.05, * *<P0.01, * * * P<0.001 with model group.
Regression analysis:
0.5h: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=9.17
1h: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=15.2
2h: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=9.57
3h: optimal conditions: X1=0.25h, X2=31.64 doubly expect result of the test: Y=5.47
4h: optimal conditions: X1=0.25h, X2=31.93 doubly expect result of the test: Y=4.91
6h: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=18.4
3.2.3 to the granulomatous influence of Oleum Tiglii
1 all body weight do not increase after the modeling of model group rat, and counter have the trend of alleviating, and it is more to ooze out liquid measure, and granulation hyperplasia is remarkable.Radix Aconiti Lateralis 15min group, 1h group, 2h group and 4h group administration 1 week back body weight significantly increase, 15min group, 30min group and 6 hours groups are oozed out liquid measure and are significantly reduced, granulation hyperplasia all the other each groups except that 2 hours organize also significantly alleviate, and compare with model group, and significance or utmost point significant difference are arranged.Through regression analysis, the optimal time dosage of Radix Aconiti Lateralis performance drug effect is body weight: 2.274h, 38.22 times; Transudate: 0.25h, 1 times; Granuloma: 0.25h, 48 times.The result sees table 23 and regression analysis for details.
Table 23 pair Oleum Tiglii causes the influence of scorching rat
Group |
Animal (only) |
Dosage (g/kg) |
Body weight (g) |
Transudate (ml) |
Granuloma (g) |
Before the medicine |
Behind the medicine |
The positive group of model group |
8 8 8 10 9 lO 10 10 9 |
Distilled water 0.0015 6 0.75 12 3 0.25 9 1.5 |
220.70±21.75 217.80±19.66 222.70±15.85 208.10±18.05 212.70±13.08 218.10±16.00 215.30±16.03 212.40±17.97 215.20±19.90 |
213.25±27.65 176.00±12.39** 239.50±14.14* 226.80±24.39 245.00±10.52** 246.00±21.64* 234.60±29.22 244.90±18.02** 239.44±29.01 |
0.613±0.368 0.044±0.062*** 0.006±0.018*** 0.015±0.034*** 0.578±1.343 1.163±2.019 0.341±0.637 0.325±0.559 0.067±0.200** |
6.16±2.64 1.02±1.03*** 1.06±0.48*** 1.70±0.92*** 2.17±1.89** 4.85±3.34 2.52±1.86** 2.44±2.27** 1.52±0.73*** |
Radix Aconiti Lateralis |
15min 30min 1h 2h 3h 4h 6h |
Annotate: compare * P<0.05, * *<P0.01, * * *<P0.001 with model group.
Regression analysis:
Body weight: optimal conditions: X1=2.274h, X2=38.22 doubly expect result of the test: Y=4.37
Transudate: optimal conditions: X1=0.25h, X2=1 doubly expect result of the test: Y=6.3
Granulation is heavy: optimal conditions: X1=0.25h, X2=48 doubly expect result of the test: Y=7.07
3.3 analgesic test
3.3.1 writhing test
Pain is obvious behind model group injected in mice 0.6% acetic acid, shows as that to turn round the body number of times frequent.Radix Aconiti Lateralis is except that the 4h group, and all the other each time dosage groups are turned round the body number of times and significantly reduced, and compares with model group, and dominance work or utmost point significant difference are arranged.Through regression analysis, the Best Times dosage of Radix Aconiti Lateralis performance drug effect be 0.25 hour 27.88 times.The result sees table 24 and regression analysis for details.
Table 24 Dichlorodiphenyl Acetate causes the influence of pain mice
Group |
Animal (only) |
Dosage (g/kg) |
Incubation period (second) |
Turn round the body number of times (inferior/15min) |
The positive group of model group |
10 10 |
Distilled water 0.1 |
218.60±47.28 900.0±0.00*** |
43.70±7.10 0.00±0.00*** |
Radix Aconiti Lateralis |
15min 30min 1h 2h 3h 4h 6h |
10 10 10 10 10 10 10 |
6 0.75 12 3 0.25 9 1.5 |
274.30±72.95 279.20±85.89 397.90±280.38 283.50±104.62 203.90±80.65 241.40±112.17 317.40±214.61 |
17.10±9.19*** 24.20±9.41*** 19.90±13.99*** 28.40±9.14*** 31.10±12.51* 34.80±15.80 22.60±11.30*** |
Annotate: compare * P<0.05, * *<P0.01, * * * P<0.001 with model group.
Regression analysis:
Optimal conditions: X1=0.25h, X2=27.88 doubly expect result of the test: Y=7.28
3.3.2 hot plate test
Radix Aconiti Lateralis is except that 30 minutes organize, and all the other respectively organize pain threshold and model group more all has significantly or utmost point significant difference.Through regression analysis, the Best Times dosage of 60 minutes time points of Radix Aconiti Lateralis performance drug effect be 0.25 hour 40.86 times.The result sees table 25 and regression analysis for details.
Table 25 pair hot plate causes the influence of pain mice pain threshold (second)
Group |
Animal (only) |
Dosage (g/kg) |
The threshold of pain, basis |
30min |
The positive group of model group |
10 10 |
Distilled water 0.1 |
18.58±3.20 18.60±4.98 |
12.58±3.13 32.64±17.49** |
Radix Aconiti Lateralis |
15min 30min 1h 2h 3h 4h 6h |
10 10 10 10 10 10 10 |
6 0.75 12 3 0.25 9 1.5 |
18.80±3.46 18.72±5.75 18.94±2.17 18.55±2.66 18.67±5.59 18.85±2.76 18.80±6.06 |
16.04±3.28* 10.54±4.31 17.35±4.98* 20.86±8.50** 16.57±3.79* 20.61±5.68** 17.02±6.75 |
|
60min |
90min |
120min |
11.15±4.47 28.15±14.12*** 16.52±4.27* 11.27±5.10 16.69±3.87** 15.02±5.40 12.64±5.87 16.35±4.43* 18.50±8.99* |
12.99±4.82 18.26±8.15 14.08±4.01 10.06±3.83 10.89±3.12 14.31±4.67 15.07±4.15 13.97±4.18 19.97±11.67 |
10.90±4.09 19.76±14.5 16.77±6.00* 11.80±5.65 11.25±1.81 11.73±3.30 17.67±7.00* 14.28±6.82 19.67±7.94** |
Annotate: compare * P<0.05, * *<P0.01 with model group.
Regression analysis:
0.5h: optimal conditions: X1=3.286h, X2=27.86 doubly expect result of the test: Y=4.42
1h: optimal conditions: X1=0.25h, X2=40.86 doubly expect result of the test: Y=3.38
1.5h: optimal conditions: X1=6h, X2=1 doubly expect result of the test: Y=2.2
2h: optimal conditions: X1=6h, X2=48 doubly expect result of the test: Y=18.4
The above-mentioned test of pesticide effectiveness proves, with water logging bubble 30min, adds to 10 times of water gagings and decocts Radix Aconiti Lateralis Preparata 15min, 2h, 5h, 6h temperature heart-yang, and 4h, 6h the kidney warming sun, 6h warming the spleen sun effect is better; With water logging bubble 30min, add to that 10 times of water gagings decoct Radix Aconiti Lateralis Preparata 15min, 5h, the 6h antiinflammatory action is better; With water logging bubble 30min, add to that 10 times of water gagings decoct Radix Aconiti Lateralis Preparata 15min, 3h, 6h analgesic effect is better.
The safety testing of test example 2 Radix Aconiti Lateralis Preparatas of the present invention (Radix Aconiti Lateralis, Radix Aconiti Lateralis Preparata, RADIX ACONITI LATERALIS PREPARATA) extract extraction process different parameters
1. give birth to Radix Aconiti Lateralis Preparata animal acute toxicity test data and documents and materials
1.1. experiment material
1.1.1. medicine
Give birth to each time point decocting liquid of Radix Aconiti Lateralis Preparata, be subjected to the reagent thing, containing the crude drug amount respectively is that 15min, 30min, 1h, 2h are 3.3g/ml, 3h is 2g/ml, and 4h is 1.33g/ml, and 6h is 1.05g/ml, the test lot number is provided by pharmaceutical college of Chengdu University of Traditional Chinese Medicine: 050622, irritate the stomach mice with each decocting liquid.
1.1.2. animal
The SPF mice, male and female half and half, body weight 18-22g is provided by Chengdu University of Traditional Chinese Medicine Animal Experimental Study center, the quality certification number: the real moving pipe matter 04-11 in river, the quarantine back is standby.
1.2. experimental technique
Prerun: get the SPF mice, male and female half and half, water 12hr is can't help in fasting, and complete dead, complete the living that records 15min, 30min by test measured, and is LD50; 1h, 2h, 3h, 4h and 6h irritate stomach, 24h successive administration 3 times with the dosage of the maximum volume 0.4ml/10g.B.W of mice tolerance.Observe 3d continuously, the record animal has non-toxic reaction and experiment front and back body weight change, is maximum tolerated dose (MTD) not produce dead dosage.
Get 100 of SPF mices, male and female half and half, water 12hr is can't help in fasting, is divided into 10 groups at random by body weight, 10 every group.Complete dead complete living at 15min, 30min set 3 dosage groups between the amount again, irritates the medicinal liquid of stomach respective concentration respectively; Observed 7 days continuously, record animal dead number of elements is asked its LD50.
Get 120 of SPF mices, male and female half and half, water 12hr is can't help in fasting, is divided into 6 groups at random by body weight, 20 every group.Be blank group (N=20), 1h group (N=20), 2h group (N=20), 3h group (N=20), 4h group (N=20) and 6h group (N=20).Irritate the medicinal liquid of stomach respective concentration, 24h successive administration 3 times respectively with the dosage of the maximum volume 0.4ml/10g.B.W of mice tolerance; Observed 7 days continuously, the record animal has non-toxic reaction and experiment front and back body weight change, is maximum tolerated dose (MTD) not produce dead dosage.
Above result all handles with the SPSS12.0 statistical software.
1.3. experimental result
1.3.1 give birth to Radix Aconiti Lateralis Preparata 15min time point medicinal liquid result
After the SPF mouse stomach is given birth to Radix Aconiti Lateralis Preparata 15min time point medicinal liquid, all occur being slow in action, dull spirit, sialemesis, turn round toxic reaction in various degree such as body, death mostly occurs dead in 24 hours after administration.The animal dead situation sees table 1 for details.
Y=-10.016+0.5095X r=0.968
LD50=19.657g/kg。
Table 26 15min gives birth to the influence of Radix Aconiti Lateralis Preparata gastric infusion to animal dead
Group |
Animal (only) |
Dosage (g/kg) |
Death toll (only) |
1 2 3 4 5 |
10 10 10 10 10 |
16.65 18.32 20.15 22.16 24.97 |
1 3 6 9 10 |
1.3.2 give birth to Radix Aconiti Lateralis Preparata 30min time point medicinal liquid result
After the SPF mouse stomach is given birth to Radix Aconiti Lateralis Preparata 30min time point medicinal liquid, all occur being slow in action, toxic reaction in various degree such as dull spirit, death mostly occurs dead in 24 hours after administration.The animal dead situation sees table 2 for details.
Y=-3.476+0.1153X r=0.561
LD50=30.151g/kg。
Table 27 30min gives birth to the influence of Radix Aconiti Lateralis Preparata gastric infusion to animal dead
Group |
Animal (only) |
Dosage (g/kg) |
Death toll (only) |
1 2 3 4 5 |
10 10 10 10 10 |
16.65 20.98 26.43 33.31 41.625 |
0 2 4 6 9 |
1.3.3 give birth to Radix Aconiti Lateralis Preparata 1h, 2h, 3h, 4h and 6h time point medicinal liquid result
After the SPF mouse stomach is given birth to other each time point medicinal liquids of Radix Aconiti Lateralis Preparata, grow healthy, fleshiness, dense and glossy by hair, be close to its body, bright and flexible, the N/R secretions of eyes, the crissum cleaning, it is normal to ingest, the extremity stalwartness, spontaneous activity is normal, and body weight increases gradually.Not interior animal dead and toxicity in the 7d.Naked eyes became celestial and do not see obvious pathological change when experiment finished.
2. Radix Aconiti Lateralis Preparata animal acute toxicity test data
2.1. experiment material
2.1.1. medicine
Each time point decocting liquid of Radix Aconiti Lateralis Preparata is subjected to the reagent thing, and containing the crude drug amount respectively is that 15min, 30min, 1h, 2h, 3h, 4h and 6h are 3.33g/ml, and the test lot number is provided by pharmaceutical college of Chengdu University of Traditional Chinese Medicine: 050625, irritate the stomach mice with each decocting liquid.
2.1.2. animal
The SPF mice, male and female half and half, body weight 18-22g is provided by Chengdu University of Traditional Chinese Medicine Animal Experimental Study center, the quality certification number: the real moving pipe matter 04-11 in river, the quarantine back is standby.
2.2. experimental technique
Prerun: get some of SPF mices, male and female half and half, water 12hr is can't help in fasting, and each time point decocting liquid of 15min, 30min, 1h, 2h, 3h, 4h and 6h is irritated stomach, 24h successive administration 3 times with the dosage of the maximum volume 0.4ml/10g.B.W of mice tolerance.Observe 3d continuously, animal does not all have dead and tangible toxic reaction, and not producing dead dosage with this is maximum tolerated dose (MTD).
Get 160 of SPF mices, male and female half and half, water 12hr is can't help in fasting, is divided into 8 groups at random by body weight, 20 every group.Be blank group (N=20), 15min group (N=20), 30min group (N=20), 1h group (N=20), 2h group (N=20), 3h group (N=20), 4h group (N=20) and 6h group (N=20).Irritate the medicinal liquid of stomach respective concentration, 24h successive administration 3 times respectively with the dosage of the maximum volume 0.4ml/10g.B.W of mice tolerance; Observed 7 days continuously, the record animal has non-toxic reaction and experiment front and back body weight change, is maximum tolerated dose (MTD) not produce dead dosage.
Above result all handles with the SPSS12.0 statistical software.
2.3. experimental result
Behind each time point decocting liquid of SPF mouse stomach Radix Aconiti Lateralis Preparata 5min, 30min, 1h, 2h, 3h, 4h and 6h, grow healthy, fleshiness, dense and glossy by hair, be close to its body, bright and flexible, the N/R secretions of eyes, crissum cleaning, it is normal to ingest, the extremity stalwartness, spontaneous activity is normal, and body weight increases gradually.Not interior animal dead and toxicity in the 7d.Naked eyes became celestial and do not see obvious pathological change when experiment finished.
3. RADIX ACONITI LATERALIS PREPARATA animal acute toxicity test data
3.1. experiment material
3.1.1. medicine
Each time point decocting liquid of RADIX ACONITI LATERALIS PREPARATA is subjected to the reagent thing, and containing the crude drug amount respectively is that 15min, 30min, 1h, 2h, 3h, 4h and 6h are 3.33g/ml, and the test lot number is provided by pharmaceutical college of Chengdu University of Traditional Chinese Medicine: 0500701, irritate the stomach mice with each decocting liquid.
3.1.2. animal
The SPF mice, male and female half and half, body weight 18-22g is provided by Chengdu University of Traditional Chinese Medicine Animal Experimental Study center, the quality certification number: the real moving pipe matter 04-11 in river, the quarantine back is standby.
3.2. experimental technique
Prerun: get some of SPF mices, male and female half and half, water 12hr is can't help in fasting, and each time point decocting liquid of 15min, 30min, 1h, 2h, 3h, 4h and 6h is irritated stomach, 24h successive administration 3 times with the dosage of the maximum volume 0.4ml/10g.B.W of mice tolerance.Observe 3d continuously, animal does not all have dead and tangible toxic reaction, and not producing dead dosage with this is maximum tolerated dose (MTD).
Get 160 of SPF mices, male and female half and half, water 12hr is can't help in fasting, is divided into 8 groups at random by body weight, 20 every group.Be blank group (N=20), 15min group (N=20), 30min group (N=20), 1h group (N=20), 2h group (N=20), 3h group (N=20), 4h group (N=20) and 6h group (N=20).Irritate the medicinal liquid of stomach respective concentration, 24h successive administration 3 times respectively with the dosage of the maximum volume 0.4ml/10g.B.W of mice tolerance; Observed 7 days continuously, the record animal has non-toxic reaction and experiment front and back body weight change, is maximum tolerated dose (MTD) not produce dead dosage.
Above result all handles with the SPSS12.0 statistical software.
3.3. experimental result
Behind each time point decocting liquid of SPF mouse stomach RADIX ACONITI LATERALIS PREPARATA 5min, 30min, 1h, 2h, 3h, 4h and 6h, grow healthy, fleshiness, dense and glossy by hair, be close to its body, bright and flexible, the N/R secretions of eyes, crissum cleaning, it is normal to ingest, the extremity stalwartness, spontaneous activity is normal, and body weight increases gradually.Not interior animal dead and toxicity in the 7d.Naked eyes became celestial and do not see obvious pathological change when experiment finished.
4. experiment conclusion
The LD50 that the SPF mouse stomach is given birth to Radix Aconiti Lateralis Preparata 15min, 30min time point medicinal liquid is respectively 19.657g crude drug in whole/kg (be equivalent to approximately people's consumption 79 times) and 30.151g crude drug in whole/kg (be equivalent to people's consumption 121 times).1h, 2h MTD be 399.6g crude drug in whole/kg (be equivalent to people's consumption 1600 times), 3h MTD be 240g crude drug in whole/kg (be equivalent to people's consumption 961 times), 4h MTD be 160g crude drug in whole/kg (be equivalent to people's consumption 640 times), and 6h MTD (maximum dosage-feeding) be 126.32g crude drug in whole/kg (be equivalent to people's consumption 505 times); Dead or unusual toxic reaction does not all appear in all animals.Prompting is given birth to Radix Aconiti Lateralis Preparata decoction 15min, 30min and is still had certain toxicity, and decocting time reaches does not have overt toxicity substantially more than the 1h.
SPF mouse stomach Radix Aconiti Lateralis Preparata 15min, 30min, 1h, 2h, 3h, 4h and 6h MTD are 399.6g crude drug in whole/kg (be equivalent to people's consumption 1598 times); Dead or unusual toxic reaction does not all appear in all animals.Prompting Radix Aconiti Lateralis Preparata decocting liquid does not have overt toxicity.
SPF mouse stomach RADIX ACONITI LATERALIS PREPARATA 15min, 30min, 1h, 2h, 3h, 4h and 6h MTD are 399.6g crude drug in whole/kg (be equivalent to people's consumption 1598 times); Dead or obviously toxic reaction does not all appear in all animals.Prompting RADIX ACONITI LATERALIS PREPARATA decocting liquid does not have overt toxicity.
The above safety of above-mentioned description of test Radix Aconiti Lateralis Preparata of the present invention (Radix Aconiti Lateralis, Radix Aconiti Lateralis Preparata, RADIX ACONITI LATERALIS PREPARATA) extract decocting time 1h.
Above-mentioned drug effect, safety test prove, adopts the product of method preparation of the present invention, and in the critical field of quality control, drug effect is clear and definite, and at different indications, reached safety, effectively, provides a kind of new selection for clinical.