CN104138450B - For treating cough and alleviating the Fructus Schisandrae Chinensis extrat of airway inflammation, its preparation method and application - Google Patents
For treating cough and alleviating the Fructus Schisandrae Chinensis extrat of airway inflammation, its preparation method and application Download PDFInfo
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Abstract
The present invention provides a kind of preparation method for treating the Fructus Schisandrae Chinensis extrat coughing and alleviating airway inflammation and application thereof.Described extract is with Fructus Schisandrae Chinensis as raw material, uses alcohol reflux 3 times, each 1 3h, and united extraction liquid filters after standing, obtains extract A；Medicinal residues soak by water three times, each 1 3h, united extraction liquid, filters after standing, obtains extract B.United extraction thing A and B, vacuum drying, obtain Fructus Schisandrae Chinensis extrat of the present invention.The preparation method processing technique of the present invention is easy, and solvent safety is nontoxic, and cheap.The present invention prepares volume Fructus Schisandrae Chinensis extrat to be had clear and definite treatment for cough and alleviates effect, and can alleviate airway inflammation, can be used for preparation treatment cough and alleviates medicine and/or the health product of airway inflammation.
Description
Technical field
The present invention relates to a kind of Chinese medicine extract, be specifically related to a kind of Fructus Schisandrae Chinensis extrat and its preparation method and application, belong to technical field of Chinese medicines.
Background technology
Cough is the modal symptom of internal clinic, sees various respiratory system and non-respiratory systemic disease.Cough itself is a kind of defense mechanism, clearly
Secretions too much in degasification road and prevent foreign body from by mistake sucking air flue.But chronic cough for a long time has had a strong impact on the quality of life of patient also
Cause the psychological burden that patient is serious, also significantly increase individual and public medical and health expenditure simultaneously.Chronic cough refers to clinically
Unique symptom or cardinal symptom, Cough length more than 8 weeks, the symptom of chest X-ray Non Apparent Abnormality.Guangzhou Inst. of Respiratory Diseases is carried out
One investigation display, Chronic Cough Patients average course of disease is 3 years, and have is decades-long, and medical average time reaches 18 times, medical therapeutic machine
Between structure average 3, wherein female patient is up to 48% because cough induces urinary incontinence ratio.In recent years along with gradually the increasing the weight of of air environmental pollution (as PM2.5,
PM10 etc.), life stress strengthen, the sickness rate of chronic cough has the trend of rising: many parts of Foreign Epidemic diseases researchs show that chronic coughs are commonly
The sickness rate of crowd about 9%～33%, Britain's Epidemiological study in 2003 shows, the chronic cough middle age sickness rate in 50-59 year reaches
12%, Chronic Cough Patients accounts for breathing the 20% of medical expert's consultation patient.In China, add up according to Guangzhou Inst. of Respiratory Diseases, in young university students
In crowd, the prevalence of chronic cough is more than 3%, thus it is speculated that the cough prevalence of community-based population will be far above these data.Chronic Cough Patients enters repeatedly
The various insignificant inspections of row, use various antibiotic and antitussive medicine treatment the most invalid, have a strong impact on physical and mental health and the Working Life of patient, and
Cause the significant wastage of public health resources.
Fructus Schisandrae Chinensis is Magnoliacea plant, fruit medicine, sour in the mouth, sweet, warm in nature.Return lung, the heart, kidney channel.On tcm clinical practice often by Fructus Schisandrae Chinensis compatibility only
Cough Chinese medicine such as Herba Ephedrae, Radix Asteris, Flos Farfarae etc., for dry cough without phlegm or chronic cough cough due to deficiency of the lung's disease endlessly in later stage of coughing.So far, always
Do not have whether document report Fructus Schisandrae Chinensis extrat can effectively treat especially chronic cough of coughing.
Summary of the invention
In order to solve the problems referred to above, the present invention provides the preparation method of a kind of Fructus Schisandrae Chinensis extrat, and the Fructus Schisandrae Chinensis extrat that the present invention prepares can be applicable to
Treatment is coughed and alleviates airway inflammation.
The present invention takes following steps to prepare Fructus Schisandrae Chinensis extrat:
1) take schisandra chinensis medicinal material, pulverize after being dried, with ethanol solution reflux, extract, 1～3 times, extract 1～3 hour every time, extract every time
Ethanol solution used volume (ml) is schisandra chinensis medicinal material quality (g) 4～8 times (that is, every 1g schisandra chinensis medicinal material adds the ethanol of 4～8ml
Solution extracts), united extraction liquid, then stand filtration, obtain extract A；
2) by step 1) extract the medicinal residues soak by water 1～3 times stayed, decoct 1～3 hour every time, the volume (ml) of the water used by decoction is every time
4～8 times of schisandra chinensis medicinal material quality (g), united extraction liquid, then stand filtration, obtain extract B；
3) combining step 1) extract the extract A and step 2 obtained) extract the extract B obtained, then it is vacuum dried, prepares described five
Taste seed extract.
Described schisandra chinensis medicinal material is Fructus Schisandrae Chinensis Schisandra chinensis (Turcz.) Baill. or schisandra chinensis Schisandra sphenanthera
At least one in Rehd.et Wils.
Described ethanol solution its be ethanol volumetric concentration ethanol water between 50-100%.
Further, step 1) in, schisandra chinensis medicinal material crushed after being dried is become 10～60 mesh.
Preferably, in step 1) after the extracting solution that obtains merges and stand and filter, also there is the step that filtrate carries out concentrating under reduced pressure；In step
2) extracting solution obtained also has, after merging and standing filtration, the step that filtrate carries out concentrating under reduced pressure.
The Fructus Schisandrae Chinensis extrat of the present invention, its preparation method is simple, equipment cost is low, energy consumption consumes less, solvent safety is nontoxic, cheap, energy
Realize industrialized production, it is easy to promote.
The present invention also provides for the Fructus Schisandrae Chinensis extrat prepared by said method or the compositions containing described Fructus Schisandrae Chinensis extrat in preparation treatment cough
Medicine and/or health product in application, described cough includes acute, subacute or chronic cough.The Fructus Schisandrae Chinensis extrat that the present invention prepares has town
Cough effect, the airway inflammation that cough is correlated with and cough hypersensitivity inhibited.
The Fructus Schisandrae Chinensis extrat of the present invention can be used alone, it is possible to adds suitable pharmaceutic adjuvant, makes soft capsule, hard capsule, tablet, granule
The various medicines pharmaceutically allowing dosage form or the health product such as agent, drop pill, watered pill, honey pill agent, oral liquid.
The Fructus Schisandrae Chinensis extrat of the present invention can also with other Chinese medicine extract form Chinese medicine composition, add suitable pharmaceutic adjuvant, make soft capsule,
The various medicines pharmaceutically allowing dosage form or the health product such as hard capsule, tablet, granule, drop pill, watered pill, honey pill agent, oral liquid.
Accompanying drawing explanation
Fig. 1, for embodiment 4 guinea pig lung tissue and air flue pathological section representative graph (HE × 200)
Fig. 2, for the impact on citric acid smoke stimulation guinea pig cough's number of times of the Fructus Schisandrae Chinensis extrat different time points
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to scope of the invention.
Embodiment 1:
The Radix Schisandrae Bicoloris medical material 10kg being dried, is ground into 30 mesh coarse powder, with 95% (v/v) alcohol reflux 3 times, each 3h, carries for three times
The volume (ml) taking ethanol used is respectively 8 times of quality of medicinal material (g), 6 times, 6 times, merges 3 extracting solution, filters after standing, decompression
Concentrate, obtain extract A；The medicinal residues soak by water stayed after extracting 2 times, each 2h, needed for twice decoction, the volume (ml) of water is respectively five
8 times of taste quality of medicinal material (g), 6 times, merge 2 extracting solution, filter after standing, concentrating under reduced pressure, obtain extract B.United extraction thing A
And B, vacuum drying, obtain Fructus Schisandrae Chinensis extrat 5.69kg.
Embodiment 2:
The Fructus Schisandrae Sphenantherae medical material 10kg being dried, is ground into 40 mesh coarse powder, 80% (v/v) ethanol water reflux, extract, 3 times, each 3h, and three
The volume (ml) of secondary extraction ethanol is respectively 8 times of schisandra chinensis medicinal material quality (g), 6 times, 6 times, merges 3 extracting solution, filters after standing,
Concentrating under reduced pressure, obtains extract A；The medicinal residues soak by water stayed after extraction 2 times, each 2h, the volume (ml) decocting water for twice is respectively the five tastes
8 times of sub-quality of medicinal material (g), 6 times, merge 2 extracting solution, filter after standing, concentrating under reduced pressure, obtain extract B.United extraction thing A
And B, vacuum drying, obtain Fructus Schisandrae Chinensis extrat 5.28kg.
Embodiment 3:
The Radix Schisandrae Bicoloris medical material 10kg being dried, is ground into 50 mesh coarse powder, 60% (v/v) ethanol water reflux, extract, 2 times, each 3h, two
The volume (ml) of secondary extraction ethanol is respectively 8 times of schisandra chinensis medicinal material quality (g), 6 times, united extraction liquid, filters, concentrating under reduced pressure after standing,
Obtain extract A；The medicinal residues soak by water that extraction stays 2 times, each 2h, the volume (ml) decocting water for twice is respectively schisandra chinensis medicinal material quality (g)
8 times, 6 times, united extraction liquid, after standing filter, concentrating under reduced pressure, obtain extract B.United extraction thing A and B, vacuum drying, obtain five
Taste seed extract 5.42kg.
Embodiment 4:
Laboratory animal
Pure white Hartly Cavia porcellus, regular grade, male, body weight 300 ± 50 g, purchased from Guangdong Province's Experimental Animal Center, credit number: SCXK (Guangdong)
2008-0002.Raise in National Key Laboratory of Guangzhou Inst. of Respiratory Diseases animal center, credit number: SYXK (Guangdong) 2008-0093.Globefish
Mus is raised in relative clean, peace and quiet, constant temperature (23 ± 3 DEG C), constant humidity (55 ± 15%) environment, and day and night the cycle is 12:12 hour/day, point cage
Raise, 5/cage, can freely obtain food and water.
Medicine and reagent
Fructus Schisandrae Chinensis water extract (prepare according to following flow process: schisandra chinensis medicinal material pulverizes → 8 times amount soak by water 2h, decoct 3 times → decoction liquor altogether and concentrate,
It is dried → Fructus Schisandrae Chinensis water extract)；0.9% sodium chloride injection: Dongguan Pu Ji pharmaceutcal corporation, Ltd.Pentobarbital sodium: Merck company of the U.S..
Codeine: Industries, Inc of traditional Chinese medicines group." red rose " board cigarette with filter tip: Guangzhou Zhong Yan Industrial Co., Ltd.Other conventional reagent: wide
State chemical reagent factory.
In detailed description of the invention, all data represent with " means standard deviation ", compare employing spss13.0 and carry out one factor analysis of variance between each group, side
Difference is neat uses least significant difference method (LSD), and heterogeneity of variance or nonnormal data acquisition rank test, P ＜ 0.05 represents that difference is statistically significant.
Experimental apparatus
PM type single-chamber certain non-limiting examples petty action object retouches instrument (1.0 L): Buxco company of the U.S.；Digital photography integral system: Nikon company of Japan；
0.6m × 0.6m × 1m smoke from cigarette toxicant exposure box: this laboratory is made by oneself；A1003 electronic balance: Satorius company of Germany；MDF-U53V-80℃
Cryogenic refrigerator: SANYO GS company；3-18K SIGMA High speed refrigerated centrifuge: Beckman company of the U.S.；22 G venous detaining needles: Suzhou is cured
Treat apparatus company limited；The common high speed centrifuge of Anke TGL-16B type: Town in Shanghai stops scientific instrument factory；The 400 desk-top refrigerated centrifuges of type: Germany
Heraeus company；XW80A type vortex agitator: Shanghai precision instrumentation company limited；DK8A thermostat water bath: the upper grand test apparatus of Nereid
Company limited.
(1) smoke from cigarette is stimulated cause Cough reflex sensitivity to increase the pharmacological action of Cavia porcellus (CS Cavia porcellus) by the Fructus Schisandrae Chinensis extrat of embodiment 1 preparation
Fume smoking method: Cavia porcellus, after experiment prospective adaptation peace and quiet raise one week, is placed in self-control medicated cigarette toxicant exposure box, lights 10 medicated cigarettes in sootiness every time
In groove (fire-cured tobacco type, tar content 12mg, smoking nicotine content 1.1mg, carbon monoxide in flue gas content 15mg), by three-way valve by smoke from cigarette
Squeeze in toxicant exposure box, start timing from medicated cigarette burning-out.Every time fumigation 20 minutes, every day fumigation 2 times, every secondary fumigation interval time more than 6 hours,
Fumigation 14 days continuously.
Experiment: Cavia porcellus completely random is divided into normal group, model group, solvent control group (PEG400 of 0.5%), codeine group, water extract height agent
Amount group (dosage 500mg/kg Cavia porcellus/sky, drug level 50mg/mL), Fructus Schisandrae Chinensis extrat high dose group (dosage 500mg/kg Cavia porcellus/sky, medicine
Substrate concentration 50mg/mL), dosage group (dosage 250mg/kg Cavia porcellus/sky, drug level 25mg/mL), Fructus Schisandrae Chinensis extrat in Fructus Schisandrae Chinensis extrat
Low dose group (dosage 125mg/kg Cavia porcellus/sky, drug level 12.5mg/mL).
In addition to normal group, remaining each treated animal all uses above-mentioned fume smoking method to stimulate.In addition to normal group and codeine group, remaining respectively organizes Cavia porcellus from sootiness
Daystart, first 2 hours per os of the second time sootiness every day give corresponding medicine or solvent, continuous 14 days.Codeine group is coughed in citric acid
Sensitivity of coughing checks first 1 hour, oral administration (30mg/kg Cavia porcellus, codeine concentration 3mg/mL).Normal group is normally raised, and does not make any place
Reason.Cavia porcellus being placed in body in 15th day and retouch in case, after adapting to 2min, atomization 0.4mol/L citric acid carries out cough and excites, and after 10min, atomization terminates,
Continuing to observe 10min, record is guinea pig cough's total degree and cough latent period in the 20min that atomization starts.
After cough excitation experiment 24h, 3% Nembutal sodium solution (1mL/kg) intraperitoneal injection of anesthesia animal, heart extracting blood 10mL.Take 6mL 0.1
The PBS of mol/L pre-cooling, divides and carries out alveolar wass for 3 times, and the unified left lung of lavation, lavation 3 times, obtain bronchoalveolar lavage fluid the most repeatedly
(Bronchoalveolar Lavage Fluid, BALF).Gained BALF is shaken suspendible, extracts 0.5mL, fully crack with erythrocyte cracked liquid,
3000 revs/min are centrifuged 10 minutes, take 10 μ L of supernatant drops and enter blood cell counting plate, calculate BALF inflammatory cell sum (cell under optical microscope
Sum/mL=(four big lattice total cellular score/4) × 104/mL)；After extraction 0.5mL, remaining BALF is centrifuged 10min, precipitation 1mL in 3000rpm
PBS suspendible, takes 100 μ L and makes cell smear, natural air drying, be soaked in 10% neutral formalin and overnight fix, carry out flowing water punching after taking-up successively
Wash 10min, haematoxylin dyeing 15sec, flowing water rinse 1min, 1% hydrochloride alcohol decolouring 2sec, flowing water rinse 10min, eosin stains 5 sec,
Flowing water rinses 10min, drying, obtains neutral gum mounting, and under optical microscope, 20 400 times of visuals field of continuous random extraction carry out continuous counter, every
400 cells of smear continuous counter, calculate the ratio of wherein neutrophilic granulocyte, eosinophilic granulocyte, macrophage, lymphocyte.
After Cavia porcellus extraction BALF, inject 50mL PBS from heart and carry out lavation, inject 2.5mL4% paraformaldehyde, then clip one piece from trachea
Bottom right lung tissue and one section of trachea, 10% neutral formalin fixes more than 24h.Take out the lung tissue that fixed, carry out successively Gradient elution using ethanol,
Biological clarifier processs, waxdip, embed, cut into slices, dewax, the of short duration rinsing of graded ethanol aquation, distilled water, after finally carrying out HE dyeing, neutrality
Natural gum mounting, carries out pathological observation.
Experimental result: compare with normal group, each experimental group Guinea Pig Airway resistance has no notable difference, points out this Experimental Modeling not cause Cavia porcellus gas
Road resistance increases, and is different from chronic obstructive pulmonary disease animal model.
Each experimental group Cavia porcellus citric acid excites cough number of times to be shown in Table 1.Result shows, compares with model group, Fructus Schisandrae Chinensis extrat (high, medium and low dose
Amount group) all can substantially reduce the cough number of times of model guinea pigs.
The Fructus Schisandrae Chinensis extrat of table 1 embodiment 1 preparation on the impact of CS guinea pig cough's number of times () (n=7)
Note: compare with normal group: * * P < 0.01；Compare with model group: #P < 0.05, ##P < 0.01
Each experimental group Cavia porcellus citric acid cough excitation experiment cough latent period is shown in Table 2.Result shows, compares with model group, the height of Fructus Schisandrae Chinensis extrat,
In, low dose group cough latent period significantly extends；Water extract high dose is inconspicuous to the effect of cough latent period.
Table 2 embodiment 1 preparation Fructus Schisandrae Chinensis extrat impact preclinical on CS guinea pig cough () (n=7)
Note: compare with normal group: * * P < 0.01；Compare with model group: #P < 0.05, ##P < 0.01
In each test group of animals bronchoalveolar lavage fluid, inflammatory cell sum and classification comparative experiments the results are shown in Table 3.Result shows, with model group ratio
Relatively, the high, medium and low dosage of Fructus Schisandrae Chinensis extrat all can substantially reduce inflammatory cell sum in BALF, substantially reduce neutrophilic granulocyte ratio in BALF.
Water extract high dose is inconspicuous to inflammatory cell sum and neutrophilic granulocyte proportional action in BALF.
Table 3 embodiment 1 preparation Fructus Schisandrae Chinensis extrat on CS guinea pig bronchial bronchoalveolar lavage fluid inflammatory cell sum and classification impact () (n=7)
Note: compare with normal group: * * P < 0.01；Compare with model group: #P < 0.05, ##P < 0.01
Comparing with model group, Fructus Schisandrae Chinensis extrat of the present invention each dosage group guinea pig lung tissue and trachea pathology are all obviously improved: Cavia porcellus peribronchiolitis
Disease cellular infiltration significantly reduces, and oozes out and alleviate in tube chamber, the broadening lesser extent of alveolar septum, and the Fructus Schisandrae Chinensis extrat that the prompting present invention prepares can be notable
Alleviate airway inflammation of guinea.And water extract high dose group guinea pig lung tissue and trachea pathology improve inconspicuous.Each experimental group guinea pig lung tissue and trachea
Pathological section representative graph is shown in accompanying drawing 1.
(2) Fructus Schisandrae Chinensis extrat of embodiment 2 preparation causes the pharmacological action of acute cough Cavia porcellus to citric acid smoke stimulation
After Cavia porcellus rests one week, carry out Cough reflex sensitivity screening: the atomization of 0.8mol/L citric acid stimulates 1min, stops atomization, continue to observe 5min,
Record starts the cough number of times in 6min from atomization, and more than 10 times, the Cavia porcellus less than 50 times is qualified Cavia porcellus.
It is divided into normal group, solvent control group (PEG400 of 0.5%), codeine group, water extract high dose by screening qualified Cavia porcellus completely random
Group (dosage 500mg/kg Cavia porcellus/sky, drug level 50mg/mL), Fructus Schisandrae Chinensis extrat high dose group (dosage 500mg/kg Cavia porcellus/sky, medicine
Concentration 50mg/mL), dosage group (dosage 250mg/kg Cavia porcellus/sky, drug level 25mg/mL) in Fructus Schisandrae Chinensis extrat, Fructus Schisandrae Chinensis extrat low
Dosage group (dosage 125mg/kg Cavia porcellus/sky, drug level 12.5mg/mL).In addition to normal group and codeine group, remaining respectively organizes Cavia porcellus continuous 5 days
Every day, per os gave medicine or solvent, 1 times/day, within 5th, is administered or after solvent, 1h carries out citric acid cough and excites: 0.8mol/L citric acid atomization thorn
Swashing 1min, stop atomization, continue to observe 5min, record starts the cough number of times in 6min from atomization.Codeine group is coughed in citric acid on the 5th
Excite first 1 hour oral administration.Normal group is normally raised, and within 5th, directly carries out citric acid cough and excites.
The cough number of times of each experimental group is as shown in table 5.Result shows, compares with normal group, Fructus Schisandrae Chinensis extrat high, medium and low dosage intervention group
Guinea pig cough's number of times all substantially reduces, and water extract high dose group guinea pig cough's number of times is without significant difference.
Table 5 embodiment 2 preparation Fructus Schisandrae Chinensis extrat on citric acid cause acute cough's guinea pig cough's number of times impact () (n=7)
Note: compare with normal group: * P < 0.05, * * P < 0.01
The cough latent period of each experimental group is as shown in table 6.Result shows, compares with normal group, Fructus Schisandrae Chinensis extrat high, medium and low dosage group Cavia porcellus
Cough latent period significantly extend.The cough latent period of water extract high dose group Cavia porcellus and normal group are without significant difference.
Table 6 embodiment 2 preparation Fructus Schisandrae Chinensis extrat on citric acid cause the preclinical impact of acute cough guinea pig cough () (n=7)
Note: compare with solvent control group: * P < 0.05, * * P < 0.01
(3) Fructus Schisandrae Chinensis extrat of embodiment 3 preparation causes the ageing of acute cough Cavia porcellus to citric acid smoke stimulation
After Cavia porcellus rests one week, carry out Cough reflex sensitivity screening: the atomization of 0.8mol/L citric acid stimulates 1min, stops atomization, continue to observe 5min,
Record starts the cough number of times in 6min from atomization, and more than 10 times, the Cavia porcellus less than 50 times is qualified Cavia porcellus.
Screen after qualified Cavia porcellus normally raises 3 days, be randomly divided into 4 big groups, every big group 30.First big group is randomly divided into 3 groups, the least
Organize 10, the most directly carry out citric acid cough and excite: the atomization of 0.8mol/L citric acid stimulates 1min, stops atomization, continue to observe 5min, note
Record starts the cough number of times in 6min from atomization.Second largest group is randomly divided into 3 groups: normal group, codeine group (30mg/kg, oral administration),
The Fructus Schisandrae Chinensis extrat group (250mg/kg, drug level 25mg/mL, oral administration) of embodiment 3 preparation, every group 10, normal group is straight
Tapping into the cough of row citric acid to excite, codeine group and Fructus Schisandrae Chinensis extrat group carry out cough after being administered 1 hour and excite, and shooting condition is with the first big group；
The third-largest group all identical with second largest group with the packet of the fourth-largest group and shooting condition, and codeine group and Fructus Schisandrae Chinensis extrat group administration time are respectively and swash
3h and 5h before sending out.
Result is as in figure 2 it is shown, can substantially reduce lemon after Fructus Schisandrae Chinensis extrat (250mg/kg) the once daily 1h for preparing of the embodiment 3 that shows
Lemon acid smoke stimulation causes guinea pig acute cough number of times, and remains to show significant drug effect after being administered 5h, and codeine is coughed after being administered 5h
Cough number of times and normal group without significant difference.
The pharmacological experiments of Fructus Schisandrae Chinensis extrat prepared by 3 embodiments shows, the Fructus Schisandrae Chinensis extrat that the present invention prepares can significantly extend CS globefish
Mus and citric acid stimulate the cough latent period of cause acute cough Cavia porcellus, reduce cough number of times, substantially reduce inflammatory cell in CS Cavia porcellus bronchoalveolar lavage fluid
Sum and neutrophilic granulocyte ratio, after the Fructus Schisandrae Chinensis extrat that the pathology section examination prompting present invention prepares is intervened, Cavia porcellus peribronchiolitis disease cell soaks
Profit significantly reduces, and oozes out and alleviate in tube chamber, and the broadening degree of alveolar septum alleviates, and points out Fructus Schisandrae Chinensis extrat of the present invention to have the airway inflammation of Cavia porcellus
Significantly inhibitory action.And the effect of Fructus Schisandrae Chinensis extrat prepared by the present invention is significantly better than Fructus Schisandrae Chinensis water extract, and in a certain amount of effect relationship,
Application prospect in terms of showing antitussive, alleviation cough and alleviating airway inflammation.
Embodiment 5:
The Fructus Schisandrae Chinensis extrat 1kg of Example 2 preparation, by suitable quantity of water, heated and boiled is dissolved, is made Fructus Schisandrae Chinensis extrat solution, standby；Separately
Take sucrose 1kg, add water boil, the Fructus Schisandrae Chinensis extrat solution prepared before addition, it is added thereto to sodium benzoate 4 g simultaneously, mixing,
Stand, take supernatant, add pure water to 4000mL, mixing, filter, sterilizing, every bottle of 100ml subpackage, make the syrup of Fructus Schisandrae Chinensis extrat.
Can instructions of taking for reference be: every day 1-3 time, each 20ml.
Embodiment 6:
The Fructus Schisandrae Chinensis extrat syrup that embodiment 5 prepares, through clinical experiment, wherein has Chronic Cough Patients 15 example and acute cough patient 15 example,
By drinking the syrup 1～3 times that embodiment 5 prepares, each 20mL every day, taking 1～2 week continuously, total effective rate is 100%, specifically feeds back
Result can see table:
| Case | Effective | Effectively | Invalid |
| Acute cough | 12 | 3 | 0 |
| Chronic cough | 11 | 4 | 0 |
Wherein 4 model cases describe in detail:
Case 1: Zhang, man, 55 years old, coughs about 4 months, is diagnosed as high quick syndrome of coughing, take the five tastes that embodiment 5 prepares continuously
Seed extract syrup one week, Cough reflex sensitivity significantly lowers, and cough number of times substantially reduces.
Case 2: Liu, female, 35 years old, coughs about 2 months, is diagnosed as postinfectious cough, take the Fructus Schisandrae Chinensis extrat that embodiment 5 prepares
2 bottles of syrup, onset in first day of taking medicine, after taking 2 bottles, cough is basic alleviates.
Case 3: Bao, female, 41 years old, cough about 1 month, the stifled sense of companion's expectorant, expectorant difficulty expectoration, it is diagnosed as postinfectious cough, takes embodiment
52 bottles of the Fructus Schisandrae Chinensis extrat syrup prepared, onset in latter second day of taking medicine, after taking 2 bottles, cough takes a turn for the better 7 one-tenth, and after adding 1 bottle, cough is basic
Complete incidence graph.
Case 4: especially certain, man, 24 years old, cough about 2 years, dry cough is main, is diagnosed as Eosinophilic's bronchitis, gastroesophageal reflux
Property cough, take 2 bottles of the Fructus Schisandrae Chinensis extrat syrup that embodiment 5 prepares, onset in the 3rd day of taking medicine, cough takes a turn for the better 4 one-tenth, after adding 2 bottles, coughs
Symptom of coughing substantially eliminates.
From above-described embodiment, only just can effectively alleviate cough by oral Fructus Schisandrae Chinensis extrat syrup of the present invention, alleviate airway inflammation.And,
The Fructus Schisandrae Chinensis extrat that the present invention prepares extracts from Chinese medicine Schisandra chinensis, Fructus Schisandrae Chinensis be Ministry of Public Health announce the Chinese medicine that can be used for health food, to human body without
Any toxicity and side effect.
Fructus Schisandrae Chinensis extrat of the present invention is except making syrup, and those skilled in the art can also use existing preparation process to be made into pharmacy as required
Upper other dosage forms acceptable, such as tablet, hard capsule, soft capsule, powder, tincture, pill, granule, injection or other oral solutionses
Type etc..Such as can make granule by the following method: the Fructus Schisandrae Chinensis extrat present invention prepared and supplementary product starch, sucrose and disintegrating agent enter
Row mixing, then feed the mixture into and mix with in water-soluble adhesive fiber element derivative solution, use extrusion granulator method to be made by medicine soft
Grain, drying, sieve, oversize is suitably pulverized, after sieve, can be made into single-size.Also tablet can be made by the following method: by the present invention
Fructus Schisandrae Chinensis extrat and appropriate amount of starch, Pulvis Talci mixing, moisten with 70% ethanol, make granule, then with tabletting machine, thus make the five tastes
Seed extract sheet.Also by adding appropriate carboxymethyl cellulose, cyclodextrin in Fructus Schisandrae Chinensis extrat of the present invention, prepare Fructus Schisandrae Chinensis extrat glue
Capsule.These preparation process are the art prior art, and this is no longer going to repeat them.
The above, be only presently preferred embodiments of the present invention, and the present invention not does any pro forma restriction, therefore all without departing from skill of the present invention
The content of art scheme, the technical spirit of the foundation present invention, to any simple modification made for any of the above embodiments, equivalent variations and modification, all still falls within this
In the range of inventive technique scheme.
Claims (8)
1. prepare for treating cough and alleviating the method for Fructus Schisandrae Chinensis extrat of airway inflammation for one kind, it is characterised in that include as
Lower step:
1) take schisandra chinensis medicinal material, pulverize after being dried, with ethanol solution reflux, extract, 1～3 times, extract 1～3 every time
Hour, volume ml is schisandra chinensis medicinal material quality g 4～8 times of the ethanol solution used by extraction every time, united extraction liquid, then
Stand and filter, filtrate is carried out concentrating under reduced pressure and obtains extract A；Described schisandra chinensis medicinal material is Fructus Schisandrae Chinensis Schisandra chinensis
(Turcz.) at least one in Baill or schisandra chinensis Schisandra sphenanthera Rehd.et Wils.；
2) by step 1) extract the medicinal residues soak by water 1～3 times stayed, decoct 1～3 hour, every time the water used by decoction every time
Volume ml is schisandra chinensis medicinal material quality g 4～8 times, united extraction liquid, then stand filtration, filtrate reduced pressure dense
Contract to obtain extract B；
3) combining step 1) extract the extract A and step 2 obtained) extract the extract B obtained, then carry out vacuum and do
Dry, prepare described Fructus Schisandrae Chinensis extrat.
Method the most according to claim 1, it is characterised in that described ethanol solution its be that ethanol volumetric concentration is at 50-100%
Between ethanol water.
Method the most according to claim 1, it is characterised in that step 1) in, schisandra chinensis medicinal material crushed after being dried is become
10～60 mesh.
4. the Fructus Schisandrae Chinensis extrat prepared by the method described in any one of claims 1 to 3.
5. the Fructus Schisandrae Chinensis extrat that method described in any one of claims 1 to 3 prepares preparation be used for treating acute, subacute or
Application in the medicine of chronic cough or health product.
Application the most according to claim 5, it is characterised in that described Fructus Schisandrae Chinensis extrat is used for antitussive, alleviation in preparation
Cough or alleviate in the medicine of airway inflammation or health product is applied.
Application the most according to claim 6, it is characterised in that described Fructus Schisandrae Chinensis extrat makes the dosage form pharmaceutically allowed.
Application the most according to claim 7, it is characterised in that described dosage form be tablet, hard capsule, soft capsule, powder,
Tincture, oral liquid, syrup, granule, pill or injection.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410387092.3A CN104138450B (en) | 2014-08-07 | 2014-08-07 | For treating cough and alleviating the Fructus Schisandrae Chinensis extrat of airway inflammation, its preparation method and application |
| PCT/CN2015/076076 WO2016019736A1 (en) | 2014-08-07 | 2015-04-08 | Schisandra extract for treating coughs and alleviating airway inflammations, and preparation method and use thereof |
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| CN201410387092.3A CN104138450B (en) | 2014-08-07 | 2014-08-07 | For treating cough and alleviating the Fructus Schisandrae Chinensis extrat of airway inflammation, its preparation method and application |
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| CN103977019B (en) * | 2014-05-16 | 2016-08-24 | 广州医科大学附属第一医院 | Fruit of Chinese magnoliavine total starches is used for treating the application in the medicine of cough or health products in preparation |
| CN104138450B (en) * | 2014-08-07 | 2016-08-03 | 广州医科大学附属第一医院 | For treating cough and alleviating the Fructus Schisandrae Chinensis extrat of airway inflammation, its preparation method and application |
| KR102169920B1 (en) * | 2019-12-20 | 2020-10-26 | 문경시 | A composition comprising extract of Schisandra chinensis, pear and Platycodon grandiflorum for preventing or treating respiratory inflammatory diseases |
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| KR100228741B1 (en) * | 1997-01-10 | 1999-11-01 | 한수길 | Solid type-food containing extract of the maximowiczia chinensis fruit |
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| CN104138450B (en) * | 2014-08-07 | 2016-08-03 | 广州医科大学附属第一医院 | For treating cough and alleviating the Fructus Schisandrae Chinensis extrat of airway inflammation, its preparation method and application |
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| CN1435206A (en) * | 2002-01-30 | 2003-08-13 | 贵州仙灵药业股份有限公司 | Sedative jujube kernel capsule |
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| WO2016019736A1 (en) | 2016-02-11 |
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