CN1931353A - Curcuma extract and its prepn process, medicine composition and use - Google Patents
Curcuma extract and its prepn process, medicine composition and use Download PDFInfo
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Abstract
The present invention is one kind of curcuma extract and its preparation process, medicine composition and use. The curcuma extract is produced with fresh curcuma rhizome, and through air drying, crushing to obtain curcuma powder, heating reflux or percolating extract for 2-4 times with organic solvent and/or water, and concentrating and drying the extracted liquid. The curcuma extract has obvious functions of inhibiting platelet aggregation caused by collagen and adrenaline, diminishing inflammation, lowering blood fat, antagonizing tumor, protecting gastric mucous membrane, antagonizing early pregnancy and benefiting gall bladder. It has certain treating effect on some cardiovascular system diseases, gynecologic diseases, surgical diseases and autoimmune diseases.
Description
Technical field: the present invention relates to a kind of Rhizoma Curcumae Longae extract and preparation method thereof, pharmaceutical composition and purposes, belong to technical field of pharmaceuticals.
Background technology: Rhizoma Curcumae Longae (Curcuma longa L.) is the dry rhizome of zingiberaceous plant Rhizoma Curcumae Longae, is the peculiar medicine commonly used in southwest, has long medicinal history.According to Miao ethnic group's medication experience among the people, its rhizome is pharmaceutically acceptable, has functions such as removing blood stasis circulation of qi promoting, inducing menstruation to relieve menalgia.Rhizoma Curcumae Longae both can singly be used, and also can go into compound recipe.Among the people in Miao ethnic group, compatibilities such as Rhizoma Curcumae Longae and Cortex Phellodendri 90, Radix Et Rhizoma Rhei, the Radix Angelicae Dahuricae, Radix Trichosanthis, Cortex Magnoliae Officinalis, Pericarpium Citri Reticulatae, Radix Glycyrrhizae, Rhizoma Zingiberis Recens Huang, Rhizoma Arisaematis cure mainly diseases such as muscular soreness that wind-damp numbness causes, joint congestion and swelling pain, joint stuffiness.
At present, what the extraction of Rhizoma Curcumae Longae was generally adopted is conventional decoction and alcohol sedimentation technique or alcohol extracting method, but does not appear in the newspapers as yet for the pharmaceutical research of Rhizoma Curcumae Longae extract.The applicant is through discovering extensively and profoundly, has by the Rhizoma Curcumae Longae extract that Rhizoma Curcumae Longae is obtained with organic solvent, water-containing organic solvent or water extraction and suppresses platelet aggregation effect, antiinflammatory action, effect for reducing blood fat, antitumor action, the effect of protection gastric mucosa, antiearly pregnancy, the choleretic effect that collagen and epinephrine cause.And demonstrate the effect of good curing cardiovascular system diseases, gynaecopathia, surgical diseases and autoimmune disease.
Summary of the invention:
The objective of the invention is to: a kind of Rhizoma Curcumae Longae extract and preparation method thereof, pharmaceutical composition and purposes are provided.Rhizoma Curcumae Longae extract of the present invention has platelet aggregation effect, antiinflammatory action, effect for reducing blood fat, antitumor action, the effect of protection gastric mucosa, antiearly pregnancy, the choleretic effect that significant inhibition collagen and epinephrine cause, particularly to the internal medicine pain in chest and hypochondrium; Amenorrhea, dysmenorrhea, mass in the abdomen; Traumatic injury, swelling and pain due to blood stasis, carbuncle and ulcer skin ulcer tinea; The upper limb rheumatic arthralgia has therapeutical effect.
The present invention is achieved in that the preparation method of Rhizoma Curcumae Longae extract is: with the Rhizoma Curcumae Longae rhizome dried and crushed of fresh collection, obtain the Rhizoma Curcumae Longae coarse powder, extract 2～4 times with organic solvent, water-containing organic solvent or water reflux or diafiltration then, obtain Rhizoma Curcumae Longae extract behind the extracting solution concentrate drying.
Described organic solvent is methanol, ethanol, propanol, butanols, acetone, chloroform, dichloromethane, methyl acetate, ethyl acetate, ether, petroleum ether or gasoline.
Specifically, the preparation method of Rhizoma Curcumae Longae water extract is: it is an amount of to get Rhizoma Curcumae Longae, is ground into coarse powder, add 10 times of water gagings and decoct 2 times, 2 hours for the first time, 1.5 hours for the second time, filter, merging filtrate, being concentrated into 60 ℃ of survey relative densities is 1.30, put cold, add ethanol and make that to contain the alcohol amount be 70%, standing over night, getting supernatant, to be evaporated to relative density be 1.25 thick paste, 75 ℃ of drying under reduced pressure, promptly.
The curcumol preparation method of extract is: it is an amount of to get Rhizoma Curcumae Longae, is ground into coarse powder, adds the alcohol reflux 2 times of 8 times of amounts 75%, each 2 hours, filter merging filtrate, decompression recycling ethanol, it is 1.25 thick paste that surplus liquid is concentrated into relative density, drying under reduced pressure, promptly.
Extract the Rhizoma Curcumae Longae extract that obtains by above-mentioned Rhizoma Curcumae Longae extract preparation method.
The pharmaceutical composition of forming by above-mentioned Rhizoma Curcumae Longae extract: comprise Rhizoma Curcumae Longae extract and one or more pharmaceutical carriers or excipient as active component.
Rhizoma Curcumae Longae extract of the present invention is used to prepare the medicine for the treatment of cardiovascular system diseases.
Described cardiovascular system diseases is the internal medicine pain in chest and hypochondrium.
Rhizoma Curcumae Longae extract of the present invention is used to prepare the medicine for the treatment of gynaecopathia.
Described gynaecopathia is amenorrhea, dysmenorrhea Huo mass in the abdomen.
Rhizoma Curcumae Longae extract of the present invention is used to prepare the medicine for the treatment of surgical diseases.
Described surgical diseases is traumatic injury, swelling and pain due to blood stasis or carbuncle and ulcer skin ulcer tinea.
Rhizoma Curcumae Longae extract of the present invention is used to prepare the medicine for the treatment of autoimmune disease.
Described autoimmune disease is the upper limb rheumatic arthralgia.
Rhizoma Curcumae Longae extract of the present invention can be separately or is used with pharmaceutical compositions, therefore also relates to containing as the Rhizoma Curcumae Longae extract of the effective dose of active ingredient and the pharmaceutical composition of conventional medicine excipient or adjuvant.Usually pharmaceutical composition of the present invention contains the Rhizoma Curcumae Longae extract of 0.1-90% weight.Pharmaceutical composition can prepare according to methods known in the art.If desired, Rhizoma Curcumae Longae extract and one or more solids or liquid medicine excipient and/or adjuvant can be combined, make the suitable administration form or the dosage form that can be used as human.
Rhizoma Curcumae Longae extract of the present invention or contain its pharmaceutical composition can the unit dosage form administration, route of administration can be intestinal or non-intestinal, as oral, muscle, subcutaneous, nasal cavity, oral mucosa, skin, peritoneum or rectum etc.Form of administration is tablet, capsule, drop pill, aerosol, pill, powder, solution, suspensoid, Emulsion, granule, liposome, transdermal agent, buccal tablet, inspection agent, unguentum, patch, injection, lyophilized injectable powder etc. for example.Can be ordinary preparation, slow releasing preparation, controlled release preparation and various particulate delivery system.For the unit form of administration is made tablet, can be extensive use of various carrier well known in the art: for example diluent and absorbent, as starch, dextrin, calcium sulfate, lactose, mannitol, sucrose, sodium chloride, glucose, carbamide, calcium carbonate, kaolin, microcrystalline Cellulose, aluminium silicate etc.; Wetting agent and binding agent are as water, glycerol, Polyethylene Glycol, ethanol, propanol, starch slurry, dextrin, syrup, Mel, glucose solution, mucialga of arabic gummy, gelatine size, sodium carboxymethyl cellulose, lac, methylcellulose, potassium phosphate, polyvinylpyrrolidone etc.; Disintegrating agent is as dry starch, alginate, agar powder, laminaran, sodium bicarbonate and citric acid, calcium carbonate, polyoxyethylene sorbitol fatty acid ester, dodecyl sodium sulfate, methylcellulose, ethyl cellulose etc.; The disintegrate inhibitor is as sucrose, glyceryl tristearate, cocoa butter, hydrogenation wet goods; Absorption enhancer is as quaternary ammonium salt, sodium lauryl sulphate etc.; Lubricant is as Pulvis Talci, silicon dioxide, corn starch, stearate, boric acid, liquid paraffin, Polyethylene Glycol etc.Tablet further can also be made coated tablet, for example sugar coated tablet, thin membrane coated tablet, ECT, or double-layer tablet, multilayer tablet.For pill is made in the administration unit, can be extensive use of various carrier well known in the art: for example diluent and absorbent, as glucose, lactose, starch, cocoa butter, nitrogenize vegetable oil, polyvinylpyrrolidone, Kaolin, Pulvis Talci etc.; Binding agent such as arabic gum, Tragacanth, gelatin, ethanol, Mel, liquid sugar, rice paste or batter etc.; Disintegrating agent is as agar powder, dry starch, alginate, dodecyl sodium sulfate, methylcellulose, ethyl cellulose etc.For the inspection agent is made in the administration unit, can be extensive use of various carrier well known in the art: for example ester of Polyethylene Glycol, lecithin, cocoa butter, higher alcohol, higher alcohol, gelatin, semi-synthetic glyceride etc.For capsule is made in the administration unit, the effective ingredient Rhizoma Curcumae Longae extract is mixed with above-mentioned various carriers, and the mixture that will obtain thus places hard ' Yanming ' capsules for clearing or soft capsule.Also the effective ingredient Rhizoma Curcumae Longae extract can be made microcapsule, be suspended in and form suspensoid in the aqueous medium, in the hard capsule of also can packing into or make injection and use.For injection preparation is made in the administration unit, as solution, Emulsion, lyophilized injectable powder and suspensoid, can use this area all diluent commonly used, for example water, ethanol, Polyethylene Glycol, 1, the isooctadecanol of ammediol, ethoxylation, the isooctadecanol of polyoxyization, Polyoxyethylene Sorbitol Fatty Acid Esters etc.In addition, ooze injection, can in injection preparation, add proper amount of sodium chloride, glucose or glycerol, in addition, can also add conventional cosolvent, buffer agent, pH regulator agent etc. in order to prepare etc.
In addition, as needs, also can in pharmaceutical preparation, add coloring agent, antiseptic, spice, correctives, sweeting agent or other material.
The dosage of Rhizoma Curcumae Longae extract of the present invention depends on many factors, for example will prevent or treat the character and the order of severity of disease, the sex of patient or animal, age, body weight and individual reaction, used particular compound, route of administration and administration number of times etc.Above-mentioned dosage can the single dose form or be divided into several, for example two, three or four dosage form administrations.
In order to verify the reasonability of preparation method of the present invention, the applicant has carried out experimental study and screening to the extraction process of Rhizoma Curcumae Longae, and is specific as follows:
One, the research of Rhizoma Curcumae Longae water extraction process
The principal element that influence decocts has: pulverizing medicinal materials granularity, decoction number of times, amount of water and decocting time etc., the applicant is through discovering: the extraction process that optimizes by the following method can be thought the best water extraction process of Rhizoma Curcumae Longae, and the extract curative effect of gained is best.
It is an amount of to get the Rhizoma Curcumae Longae medical material, and according to the form below 1 test is carried out the preferred of extraction process with the pharmacologically active scoring of paste-forming rate and Rhizoma Curcumae Longae as evaluation index, the results are shown in Table 2, table 3:
The factor level table of table 1 Rhizoma Curcumae Longae Study on extraction
Table 2 Rhizoma Curcumae Longae extraction process preferred result table
| Tested number | Factor | Evaluation index | ||||
| A pulverizes the order number | B decocts number of times (inferior) | C amount of water (doubly) | D decocting time (h) | Paste-forming rate (%) | The pharmacologically active scoring | |
| 1 | 1 | 1 | 1 | 1 | 12.42 | 78 |
| 2 | 1 | 2 | 2 | 2 | 11.26 | 84 |
| 3 | 1 | 3 | 3 | 3 | 14.21 | 81 |
| 4 | 2 | 1 | 2 | 3 | 13.56 | 77 |
| 5 | 2 | 2 | 3 | 1 | 11.24 | 80 |
| 6 | 2 | 3 | 1 | 2 | 13.74 | 75 |
| 7 | 3 | 1 | 3 | 2 | 10.65 | 79 |
| 8 | 3 | 2 | 1 | 3 | 12.82 | 74 |
| 9 | 3 | 3 | 2 | 1 | 13.60 | 78 |
| I | 15.6455 | 13.5432 | 10.1233 | 15.0415 | ||
| II | 13.7410 | 18.2576 | 19.4255 | 15.1254 | ||
| III | 17.8523 | 15.4125 | 17.4421 | 16.5516 | ||
| SS | 2.9634 | 13.2457 | 14.3356 | 0.3966 | ||
Table 3 orthogonal test analysis of variance table
| Soruces of variation | SS | Degree of freedom | It is mean square to make a variation | Statistic F | Significance |
| A | 2.9634 | 2 | 1.4817 | 7.47201 | |
| B | 13.2457 | 2 | 6.6229 | 33.3981 | P＜0.05 |
| C | 14.3356 | 2 | 7.1678 | 36.1462 | P＜0.05 |
| D (error) | 0.3966 | 2 | 0.1983 | 1 | |
| Amount to | 30.9413 | 8 |
Annotate: F
0.05(2,2)=19.00
Result of study shows: each factor influences size sequence to the Rhizoma Curcumae Longae water extraction process and is: C＞B＞A＞D, and wherein two factors of B, C have significant difference, B2＞B3＞B1, C2＞C3＞C1, therefore, extraction time selects 2 times, and amount of water selects 10 times; Two factors of A, D do not have significant difference, but consider and produce reality, select coarse powder, and with the convenient extracting solution that filters, decocting time is decided to be 2 hours for the first time, is decided to be 1.5 hours for the second time.
Therefore, the optimum extraction process of Rhizoma Curcumae Longae water extract is: it is an amount of to get Rhizoma Curcumae Longae, is ground into coarse powder, and the decocting that adds 10 times of amounts boils 2 times, 2 hours for the first time, 1.5 hours for the second time, filters merging filtrate.
The Rhizoma Curcumae Longae medical material is after the water concentration contracts, and remove impurity is quite crucial, and the applicant adopts Different concentrations of alcohol to carry out precipitate with ethanol to remove impurity, and method is as follows:
Get the Rhizoma Curcumae Longae coarse powder, carry decocting condition by preferred water and decoct extraction, (60-75 ℃ of concentrating under reduced pressure,-0.08Mpa) to relative phase density 1.30 (60 ℃), shake up, measure a certain amount of concentrated solution, add ethanol, making ethanol content is 60%, 70%, 80%, and precipitate with ethanol 24 hours is got the supernatant decompression recycling ethanol, volatilize, drying under reduced pressure (65-80 ℃ ,-0.08Mpa), claim ethanol extract weight; Alcohol hypostasis drying under reduced pressure (65-80 ℃ ,-0.08Mpa), claim impurity weight.Measure the pharmacologically active of ethanol extract, result of the test sees Table 4.
Table 4 impurity removal process result of study table
| Sequence number | Amount of liquid medicine (ml) | Contain alcohol amount (%) | Ethanol extract amount (g) | Alcohol hypostasis amount (g) | The pharmacologically active scoring |
| 1 | 125 | 60 | 11.4120 | 7.4251 | 88 |
| 2 | 125 | 80 | 10.7412 | 8.1242 | 87 |
| 3 | 125 | 70 | 10.1204 | 8.4277 | 89 |
| 4 | 125 | 0 | 19.4252 | 0 | 85 |
Result of study shows: alcohol precipitation concentration is 70% o'clock, and the impurity of removing is maximum, and simultaneously pharmacologically active is the highest, therefore, selects for use 70% concentration of alcohol as the concentration of removing impurity.
The research of concentration technology: the applicant has investigated concentrating under reduced pressure and normal pressure concentrates two kinds of methods, find through preferred, concentrating under reduced pressure and normal pressure concentrate two kinds of methods relatively, concentrating under reduced pressure is concentrated fast than normal pressure, save time, and pharmacologically active is concentrated higher than normal pressure, therefore, selects the method for concentration of concentrating under reduced pressure as the preparation of this extract.
Drying process: the applicant discovers that baking temperature has certain influence to the pharmacologically active of extract, and this extract is best at the pharmacologically active of 75 ℃ of drying under reduced pressure, therefore, selects the drying means of 75 ℃ of drying under reduced pressure as this extract.
Two, curcumol Study on extraction process
The principal element that influence is extracted has: extraction time, solvent consumption, solvent concentration and extraction time etc., the applicant is through discovering: the extraction process that optimizes by the following method can be thought the best alcohol extraction technology of Rhizoma Curcumae Longae, and the extract curative effect of gained is best.
It is an amount of to get the Rhizoma Curcumae Longae medical material, is ground into fine powder, tests by table 5, the results are shown in Table 6, table 7:
The factor level table of table 5 curcumol Study on extraction
Table 6 curcumol extraction process preferred result table
| Tested number | Factor | Evaluation index | ||||
| A extraction time (inferior) | B solvent consumption (doubly) | C concentration (%) | D extraction time (h) | Paste-forming rate (%) | The pharmacologically active scoring | |
| 1 | 1 | 1 | 1 | 1 | 10.33 | 83 |
| 2 | 1 | 2 | 2 | 2 | 11.49 | 85 |
| 3 | 1 | 3 | 3 | 3 | 10.27 | 86 |
| 4 | 2 | 1 | 2 | 3 | 12.29 | 90 |
| 5 | 2 | 2 | 3 | 1 | 12.34 | 92 |
| 6 | 2 | 3 | 1 | 2 | 12.85 | 91 |
| 7 | 3 | 1 | 3 | 2 | 13.64 | 85 |
| 8 | 3 | 2 | 1 | 3 | 13.77 | 80 |
| 9 | 3 | 3 | 2 | 1 | 13.61 | 84 |
| I | 14.8863 | 13.5421 | 10.4572 | 15.6241 | ||
| II | 18.1797 | 18.5816 | 19.4685 | 15.6984 | ||
| III | 16.1466 | 15.8459 | 17.2564 | 16.5182 | ||
| SS | 2.8759 | 12.2281 | 14.7163 | 0.4026 | ||
Table 7 orthogonal test analysis of variance table
| Soruces of variation | SS | Degree of freedom | It is mean square to make a variation | Statistic F | Significance |
| A | 2.8759 | 2 | 1.4380 | 7.1433 | |
| B | 12.2281 | 2 | 6.1141 | 30.3728 | P＜0.05 |
| C | 14.7163 | 2 | 7.3582 | 36.5532 | P＜0.05 |
| D (error) | 0.4026 | 2 | 0.2013 | 1 | |
| Amount to | 30.2229 | 8 |
Annotate: F
0.05(2,2)=19.00
Result of study shows: each factor influences size order to the curcumol extraction process and is: C＞B＞A＞D, by intuitive analysis, select C2, B2, A2, D2 as optimum extraction process, that is: Rhizoma Curcumae Longae is an amount of, be ground into coarse powder, add 8 times of amount alcohol reflux of 75% 2 times, each 2 hours, filter, it is 1.25 thick paste that merging filtrate, decompression recycling ethanol, surplus liquid are concentrated into relative density, drying under reduced pressure promptly gets the curcumol extract.
In order to ensure effect of the present invention, the applicant has carried out biological activity test to described Rhizoma Curcumae Longae extract, and is specific as follows:
Observation index: Rhizoma Curcumae Longae extract to the analgesic activity (writhing method) of white mice, Rhizoma Curcumae Longae extract to the influence of external rat whole blood platelet aggregation, to influence, the Ovum Gallus domesticus album of hyperlipemia in mice blood fat cause rat pedal swelling experiment (antiinflammatory action), to the influence of rat liver homogenate lipid peroxide effect.
The Rhizoma Curcumae Longae extract that this biological activity test adopted is with ethanol as extracting the extract that solvent obtains.
1. Rhizoma Curcumae Longae extract is to the analgesic activity (writhing method) of white mice:
Method and result: 40 of Kunming kind white mice, body weight 18-22g, ♀ ♂ half and half cuts the hair of white mice abdominal part, is divided into 2 groups at random, 20 every group.The administration group gavages Rhizoma Curcumae Longae extract 0.5g/10g, and matched group gavages the equivalent normal saline.30min after the administration, each Mus lumbar injection 0.6% acetum 0.2ml/ are only.The number of times and the time that occur writhing response behind observation and the record injection acetum in the 15-30min.Calculate the analgesia percentage rate of Rhizoma Curcumae Longae extract.The results are shown in Table 8.
The result shows: the white mice writhing response has the obvious suppression effect due to the Rhizoma Curcumae Longae extract Dichlorodiphenyl Acetate.
The influence of white mice writhing response due to table 8 Dichlorodiphenyl Acetate (x ± s)
| Group | Number of animals (only) | Drug level (%) | Turn round the body number of times in the 15min |
| The normal saline group | 20 | -- | 41.13±9.48 |
| The Rhizoma Curcumae Longae extract group | 20 | 10 | 19.28±4.33** |
2. Rhizoma Curcumae Longae extract is to the influence of external rat whole blood platelet aggregation:
Method and result: get body weight 200-250g rat, behind 3% pentobarbital sodium (0.1ml/100g) intraperitoneal injection of anesthesia, from abdominal aortic blood, injection fills the test tube (blood is 9: 1 with the ratio of anticoagulant) of 3.8% liquor sodii citratis, after shaking up gently, in teat glass at the bottom of the micro sample adding appliance absorption anticoagulation 0.5ml horizontalization, add the 0.5ml tyrode's solution again and do dilution in 1: 1, mixing, put into little bar magnet, test tube is placed in the pre-temperature hole of whole blood platelet aggregation instrument, test behind the 5-6min, during test electric platinum electrode is inserted in the test tube unlatching/stirring 0! Press/measure key 0 zeroing, press/amplitude 0 key modulation.Delivery tube adds mixing zeroing (control tube adds 20 μ l normal saline) in the anticoagulant tube with 20 μ l medicinal liquids, adds ADP10 μ l again, the resistance value (ohm) of record 5min.The results are shown in Table 9:
Table 9 Rhizoma Curcumae Longae extract is to the influence of external rat whole blood platelet aggregation (x ± s)
| Group | Matched group | Small dose group | Heavy dose of group |
| Resistance value | 3.9±0.6 | 2.2±0.8** | 1.9±0.5** |
The result shows: Rhizoma Curcumae Longae extract has significant anticoagulant effect.
3. Rhizoma Curcumae Longae extract is to the influence of hyperlipemia in mice blood fat:
Method and result: animal hyperlipemia model preparation: get the new fresh hen egg of 1-2d, remove Ovum Gallus domesticus album, add normal saline and stir and be made into 75% egg yolk emulsion, after the last medication 2h except that matched group, every Mus ip0.5ml.
Get 40 of mices, be divided into blank group, hyperlipidemia model group, Xuezhikang group and Rhizoma Curcumae Longae at random and put forward the thing group, 10 every group.Each administration group gastric infusion every day 1 time, matched group and model group give isopyknic distilled water, continuously 10d.2h after the last administration, except that matched group, all the other are respectively organized the equal lumbar injection 75% egg yolk emulsion 0.5ml/ of mice and only cause experimental hyperlipidemia.Fasting 18-20h after the lumbar injection egg yolk emulsion extracts eyeball and gets blood, presses the content that kit method detects TC, TG.The results are shown in Table 10:
The result shows: Rhizoma Curcumae Longae extract has good effect for reducing blood fat.
Table 10 Rhizoma Curcumae Longae extract is to the influence of mice hyperlipemia
| Group | Dosage (g/kg) | TC(mmol/L) | TG(mmol/L) |
| Matched group | - | 2.25±0.52 | 1.26±0.28 |
| Model group | - | 12.94±2.07 | 22.40±3.38 |
| Xuezhikang | 0.4 | 10.55±2.64** | 19.61±2.63* |
| The Rhizoma Curcumae Longae extract group | 0.8 | 12.03±2.97** | 18.64±4.74* |
Annotate: * * P＜0.01 * P＜0.05
4. Rhizoma Curcumae Longae extract causes the influence of pedal swelling behind the rat to Ovum Gallus domesticus album:
Method and result: get 30 of Wistar rat, weigh, use the picric acid labelling, be divided into 3 groups, rat right hind leg is fixed with hands, make a sign with ball pen in its ankle, the right hind of animal immerses in the sufficient capacity measuring device, and immersion depth is the boundary with the sign place.The numerical value that reads from the measuring pipette place of this device is the normal volume of the right foot of animal.Survey twice continuously, ask its meansigma methods.Irritate the following medicine of stomach respectively for 3 treated animals: the solution that Rhizoma Curcumae Longae extract makes, hydrocortisone, normal saline, capacity is 1ml/100g.Behind the 30min in every Mus right hind centre of the palm to ankle joint direction inserting needle, subcutaneous injection 10% egg clear liquid 0.1ml/ is only.Thereafter per volume of measuring the Mus right hind in 0.5 hour respectively, continuous 3 times.
Experimental result (table 11) shows that Rhizoma Curcumae Longae extract has good antiinflammatory action (stronger than hydrocortisone).
Table 11 Rhizoma Curcumae Longae extract causes the influence of pedal swelling behind the rat to Ovum Gallus domesticus album
| Group | The animal number of elements | Drug dose mgkg -1 | Cause scorching back paw swelling/ml | ||
| 0.5h | 1.0h | 1.5h | |||
| The normal saline group | 10 | - | 0.51±0.13 | 0.49±0.17 | 0.45±0.11 |
| The hydrocortisone group | 10 | 5％ | 0.37±0.14** | 0.32±0.08** | 0.26±0.13** |
| The Rhizoma Curcumae Longae extract group | 10 | 10％ | 0.29±0.12** | 0.26±0.14** | 0.19±0.15** |
Annotate: * * represents and the poor heteropole of normal saline group remarkable (P＜0.01)
5. Rhizoma Curcumae Longae extract is to the influence of rat liver homogenate lipid peroxide effect.
Method and result: get the 250-300g rat back sacrificed by decapitation of weighing, take out liver, inhale with filter paper and remove residual blood, be cut into fine grained chippings; Take by weighing 5g, add normal saline, with the homogenate (8000-10000r/min) of homogenate mechanism to 100ml.With the centrifuge tube numbering, get fresh rat liver homogenate (50mg/ml) 1.5ml and add in the centrifuge tube respectively.Experiment tube adds Rhizoma Curcumae Longae extract solution 0.1ml, and control tube adds normal saline 0.1ml, in 37 ℃ of jolting incubation 1.5h, takes out the back and adds 1.5ml trichloroacetic acid (0.2g/ml).Other establishes the blank pipe, adds the 1.5ml20% trichloroacetic acid before the incubation, with above-mentioned two kinds of same joltings of experiment tube (using when this pipe returns to zero as electrophotometer).Leave standstill 10min behind the mixing, centrifugal 50s
-1(3000rpm) min.Get supernatant 1.5ml, add the 1ml thiobarbituricacid, boiling water bath heating 10min.The cooling back is in 721 spectrophotometer 532nm wavelength colorimetric determinations.With the tetraethoxypropane is standard, calculates the wet hepatic tissue lipid peroxide growing amount of every gram.The results are shown in Table 12:
The influence that table 12 Rhizoma Curcumae Longae extract generates the rat liver homogenate lipid peroxide
| Group | Experiment number | Nmol/g (wet liver is heavy) lipid peroxidation quality |
| The Rhizoma Curcumae Longae extract group | 3 | 200-250 |
| Matched group | 3 | 500-600 |
Experimental result shows: Rhizoma Curcumae Longae extract has the obvious suppression effect to rat liver homogenate lipid peroxide growing amount.
Conclusion: above-mentioned five experimental results show that the Rhizoma Curcumae Longae extract Dichlorodiphenyl Acetate causes the mice pain reaction and has the obvious suppression effect; Has the effect of obvious suppression platelet aggregation; Has significant effect for reducing blood fat; Has good antiinflammatory action; Rat liver homogenate lipid peroxide growing amount had the obvious suppression effect.
The specific embodiment:
Embodiments of the invention 1: the preparation of Rhizoma Curcumae Longae water extract:
Get turmeric stem product 10kg, be ground into coarse powder, add 10 times of water gagings and decoct 2 times, 2 hours for the first time, 1.5 hours for the second time, filter, merging filtrate, being concentrated into 60 ℃, to survey relative densities be 1.30, puts coldly, adds ethanol and make that to contain the alcohol amount be 70%, standing over night, getting supernatant, to be evaporated to relative density be 1.25 thick paste, and 75 ℃ of drying under reduced pressure promptly get Rhizoma Curcumae Longae water extract 290g.
Embodiments of the invention 2: the preparation of Rhizoma Curcumae Longae ethanol extraction:
Get turmeric stem product 10kg, be ground into coarse powder, add 8 times of amount alcohol reflux of 75% 2 times, each 2 hours, filter merging filtrate, decompression recycling ethanol, it is 1.25 thick paste that surplus liquid is concentrated into relative density, and drying under reduced pressure promptly gets Rhizoma Curcumae Longae ethanol extraction 450g.
Embodiments of the invention 3: the preparation of Rhizoma Curcumae Longae ligroin extraction:
After turmeric stem product 10kg pulverizing, use the petroleum ether heating and refluxing extraction, use petroleum ether 40L at every turn, extract 3 times, merge extractive liquid,, reduction vaporization obtains ligroin extraction 310g to doing.
Embodiments of the invention 4: the preparation of Rhizoma Curcumae Longae ethyl acetate water solution extract:
After turmeric stem product 10kg pulverizing, with ethyl acetate aqueous solution heating and refluxing extraction, use ethyl acetate aqueous solution 60L at every turn, extract 2 times, merge extractive liquid,, reduction vaporization obtains ethyl acetate water solution extract 246g to doing.
Embodiments of the invention 5: the preparation of Rhizoma Curcumae Longae chloroform extraction thing:
After turmeric stem product 10kg pulverizing, extract with the chloroform diafiltration, use chloroform 30L at every turn, extract 4 times, merge extractive liquid,, reduction vaporization obtains chloroform extraction thing 233g to doing.
Embodiments of the invention 6: the pharmaceutical composition 1 that contains Rhizoma Curcumae Longae extract:
Take by weighing raw material by following proportioning: curcumol extract 250g, vegetable oil 200g, soil temperature-80 4g.Use the colloid mill mixing, be pressed into 1000 soft capsules.This preparation oral, 3 times on the one, is used for the treatment of cardiovascular system diseases such as internal medicine pain in chest and hypochondrium by each 4.
Embodiments of the invention 7: the pharmaceutical composition 2 that contains Rhizoma Curcumae Longae extract:
Take by weighing raw material by following proportioning: Herba Portulacae 120g, Cortex Moutan 120g, Herba Houttuyniae 150g, charred Radix Et Rhizoma Rhei 100g, Radix Astragali 120g, Caulis Bambusae In Taenia 100g, field thistle charcoal 100g, charred Radix Sanguisorbae 120g, Semen Lablab Album (parched) 120g, Flos Capsellae 120g, Caulis Sargentodoxae 150g, Radix Glycyrrhizae 50g, decoct with water secondary, each 2 hours, filter, it is 1.20 (60 ℃) that filtrate is concentrated into relative density, adding ethanol to concentration is 70%, standing over night, get supernatant, concentrate, drying under reduced pressure, dried cream mix the back and pulverize with Rhizoma Curcumae Longae extract 100g, add right amount of auxiliary materials such as starch, adopt common process to be pressed into 1000.This preparation oral, each 2-4 sheet, is used for the treatment of gynaecopathias such as amenorrhea, dysmenorrhea Huo mass in the abdomen at every day three times.
Embodiments of the invention 8: the pharmaceutical composition 3 that contains Rhizoma Curcumae Longae extract:
Take by weighing raw material by following proportioning: Radix Trichosanthis 50g, Cortex Phellodendri 50g, Radix Et Rhizoma Rhei 30g, Rhizoma Curcumae Longae 50g, Radix Angelicae Dahuricae 30g, Cortex Magnoliae Officinalis 30g, Pericarpium Citri Reticulatae 20g, Radix Glycyrrhizae 20g, Rhizoma Atractylodis 20g, Rhizoma Arisaematis 20g.Above medicine grinds altogether to loosing, and face to demonstrate,prove when using or and transfer, or transfer, or transfer, or transfer, or smash juice with Radix Isatidis and transfer with sweet with yellow wine with Sucus Allii Fistulosi with tea, or the fried cream of Oleum Sesami.Be used for the treatment of the carbuncle ancient sacrificial utensil, carbuncle on the back, all as furuncle swollen, injury from falling down, pernicious influence caused by damp-phlegm, swollen during major part, the stream skin ulcer, erysipelas, wind heat sky skin ulcer, skin is red swollen, dried beriberi caused by pathogenic damp, women's acute mastitis, surgical diseasess such as infantile erysipelas.
Embodiments of the invention 9: the pharmaceutical composition 4 that contains Rhizoma Curcumae Longae extract:
Take by weighing raw material by following proportioning: Radix Clematidis 15g, Fructus Chaenomelis 30g, Radix Angelicae Pubescentis 10g, Radix Achyranthis Bidentatae 10g, Rhizoma Curcumae Longae 10g, Ramulus Cinnamomi 10g, Caulis Lonicerae 30g, Radix Angelicae Sinensis 20g, Radix Glycyrrhizae Preparata 10g.This side prepares soup by the traditional decoction preparation method, and 1 dose of every day, head is fried in shallow oil and added water 500mL, takes medicinal liquid 200mL, and two fry in shallow oil and add water 300mL, take medicinal liquid 100mL, and two fry in shallow oil mutually and mix, and a small amount of several times decoction being taken warmly is generally taken 7～21 doses.Be used for the treatment of autoimmune diseasees such as upper limb rheumatic arthralgia.
Embodiments of the invention 10: Rhizoma Curcumae Longae extract single preparations of ephedrine:
Take by weighing Rhizoma Curcumae Longae extract 200g, vegetable oil 156.8g, Cera Flava 3.2g mixing, grind evenly,, make 1000, every 0.4g with the canned one-tenth soft capsule of pressing with colloid mill.This preparation oral, one time 4,3 times on the one.Be used for the treatment of the heart, abdomen, breast, hypochondriac pain and traumatic injury that stagnation of QI and blood causes, the brachialgia of rheumatism shoulder etc.
Claims (14)
1. the preparation method of Rhizoma Curcumae Longae extract, it is characterized in that: with the Rhizoma Curcumae Longae rhizome dried and crushed of fresh collection, obtain the Rhizoma Curcumae Longae coarse powder, extract 2～4 times with organic solvent, water-containing organic solvent or water reflux or diafiltration then, obtain Rhizoma Curcumae Longae extract behind the extracting solution concentrate drying.
2. according to the preparation method of the described Rhizoma Curcumae Longae extract of claim 1, it is characterized in that: described organic solvent is methanol, ethanol, propanol, butanols, acetone, chloroform, dichloromethane, methyl acetate, ethyl acetate, ether, petroleum ether or gasoline.
3. according to the preparation method of claim 1 or 2 described Rhizoma Curcumae Longae extracts, it is characterized in that: it is an amount of to get Rhizoma Curcumae Longae, is ground into coarse powder, add 10 times of water gagings and decoct 2 times, 2 hours for the first time, 1.5 hours for the second time, filter, merging filtrate, being concentrated into 60 ℃ of survey relative densities is 1.30, put cold, add ethanol and make that to contain the alcohol amount be 70%, standing over night, getting supernatant, to be evaporated to relative density be 1.25 thick paste, 75 ℃ of drying under reduced pressure promptly get the Rhizoma Curcumae Longae water extract.
4. according to the preparation method of claim 1 or 2 described Rhizoma Curcumae Longae extracts, it is characterized in that: it is an amount of to get Rhizoma Curcumae Longae, be ground into coarse powder, add 8 times of amount alcohol reflux of 75% 2 times, each 2 hours, filter, it is 1.25 thick paste that merging filtrate, decompression recycling ethanol, surplus liquid are concentrated into relative density, drying under reduced pressure promptly gets the curcumol extract.
5. the Rhizoma Curcumae Longae extract preparation method is extracted the Rhizoma Curcumae Longae extract that obtains according to claim 1.
6. the pharmaceutical composition that Rhizoma Curcumae Longae extract as claimed in claim 5 is formed is characterized in that: comprise Rhizoma Curcumae Longae extract and one or more pharmaceutical carriers or excipient as active component.
7. Rhizoma Curcumae Longae extract as claimed in claim 5 is used to prepare the medicine for the treatment of cardiovascular system diseases.
8. according to the purposes of the described Rhizoma Curcumae Longae extract of claim 7, it is characterized in that: described cardiovascular system diseases is the internal medicine pain in chest and hypochondrium.
9. Rhizoma Curcumae Longae extract as claimed in claim 5 is used to prepare the medicine for the treatment of gynaecopathia.
10. according to the purposes of the described Rhizoma Curcumae Longae extract of claim 9, it is characterized in that: described gynaecopathia is amenorrhea, dysmenorrhea Huo mass in the abdomen.
11. Rhizoma Curcumae Longae extract as claimed in claim 5 is used to prepare the medicine for the treatment of surgical diseases.
12. the purposes according to the described Rhizoma Curcumae Longae extract of claim 11 is characterized in that: described surgical diseases is traumatic injury, swelling and pain due to blood stasis or carbuncle and ulcer skin ulcer tinea.
13. Rhizoma Curcumae Longae extract as claimed in claim 5 is used to prepare the medicine for the treatment of autoimmune disease.
14. the purposes according to the described Rhizoma Curcumae Longae extract of claim 13 is characterized in that: described autoimmune disease is the upper limb rheumatic arthralgia.
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| CN101433692B (en) * | 2007-11-13 | 2011-08-10 | 天津天士力制药股份有限公司 | Effective component of turmeric rhizome and preparation method and use thereof |
| CN101428129B (en) * | 2007-11-06 | 2011-10-05 | 天津天士力制药股份有限公司 | Effective component of turmeric rhizome, preparation method and uses thereof |
| CN101433693B (en) * | 2007-11-13 | 2011-10-05 | 天津天士力制药股份有限公司 | Effective component of turmeric rhizome and preparation method and use thereof |
| CN102228665A (en) * | 2011-06-29 | 2011-11-02 | 烟台新时代健康产业有限公司 | Composition prepared from pine pollen and curcuma, preparation method thereof, and application of composition in preparing medicament for protecting gastric mucosa |
| CN104436067A (en) * | 2014-12-20 | 2015-03-25 | 秦文官 | Traditional Chinese medicine capsule for treating neonatal erysipelas and preparation method of traditional Chinese medicine capsule |
| CN107189884A (en) * | 2017-05-11 | 2017-09-22 | 跨越生物科技（滁州）有限公司 | A kind of handmade soap of prevention spring long tinea |
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| CN110680892A (en) * | 2018-07-04 | 2020-01-14 | 黑龙江燎原科技有限公司 | Cataplasm hot patch for relieving pain of tumor |
| CN111278301A (en) * | 2017-10-31 | 2020-06-12 | 好侍食品集团本社株式会社 | Extraction method of curcumol from myrrh |
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| CN101428129B (en) * | 2007-11-06 | 2011-10-05 | 天津天士力制药股份有限公司 | Effective component of turmeric rhizome, preparation method and uses thereof |
| CN101433693B (en) * | 2007-11-13 | 2011-10-05 | 天津天士力制药股份有限公司 | Effective component of turmeric rhizome and preparation method and use thereof |
| CN101433692B (en) * | 2007-11-13 | 2011-08-10 | 天津天士力制药股份有限公司 | Effective component of turmeric rhizome and preparation method and use thereof |
| CN102228665A (en) * | 2011-06-29 | 2011-11-02 | 烟台新时代健康产业有限公司 | Composition prepared from pine pollen and curcuma, preparation method thereof, and application of composition in preparing medicament for protecting gastric mucosa |
| CN104436067A (en) * | 2014-12-20 | 2015-03-25 | 秦文官 | Traditional Chinese medicine capsule for treating neonatal erysipelas and preparation method of traditional Chinese medicine capsule |
| CN107189884A (en) * | 2017-05-11 | 2017-09-22 | 跨越生物科技（滁州）有限公司 | A kind of handmade soap of prevention spring long tinea |
| CN111278301A (en) * | 2017-10-31 | 2020-06-12 | 好侍食品集团本社株式会社 | Extraction method of curcumol from myrrh |
| CN110680892A (en) * | 2018-07-04 | 2020-01-14 | 黑龙江燎原科技有限公司 | Cataplasm hot patch for relieving pain of tumor |
| CN109453362A (en) * | 2019-01-16 | 2019-03-12 | 汤臣倍健股份有限公司 | A kind of composition and its application for protecting stomach lining and helicobacter pylori resistant |
| CN111772188A (en) * | 2020-07-07 | 2020-10-16 | 河北智同生物制药股份有限公司 | Liver polypeptide for protecting liver and improving liver function and composition thereof |
| CN115590942A (en) * | 2022-10-18 | 2023-01-13 | 福建摩凡营养食品有限公司（Cn） | Traditional Chinese medicine composition for assisting in reducing blood fat and preparation method thereof |
| CN115590942B (en) * | 2022-10-18 | 2023-08-04 | 福建摩凡营养食品有限公司 | Traditional Chinese medicine composition for assisting in reducing blood fat and preparation method thereof |
| CN116211983A (en) * | 2023-01-17 | 2023-06-06 | 昆明理工大学 | Gastrodia elata prescription medicine for treating primary dysmenorrhea and application thereof |
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| CN100594928C (en) | 2010-03-24 |
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