CN102228665A - Composition prepared from pine pollen and curcuma, preparation method thereof, and application of composition in preparing medicament for protecting gastric mucosa - Google Patents
Composition prepared from pine pollen and curcuma, preparation method thereof, and application of composition in preparing medicament for protecting gastric mucosa Download PDFInfo
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- CN102228665A CN102228665A CN2011101779620A CN201110177962A CN102228665A CN 102228665 A CN102228665 A CN 102228665A CN 2011101779620 A CN2011101779620 A CN 2011101779620A CN 201110177962 A CN201110177962 A CN 201110177962A CN 102228665 A CN102228665 A CN 102228665A
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Abstract
The invention provides a composition prepared from pine pollen and curcuma, a preparation method thereof, and application of the composition in preparing a medicament for protecting the gastric mucosa, relating to a composition prepared from plants and a preparation method and application thereof. The composition provided by the invention can alleviate gastric ulcer caused by ethanol, indomethacin, acetic acid and the like, and can be used for preparing a medicament for protecting the gastric mucosa. The composition comprises the following components in parts by weight: 30-39 parts of wall-broken pine pollen and 1 part of curcuma extract, wherein the curcuma extract is composed of curcumin, demethoxycurcumin and bisdemethoxycurcumin in mass ratio of (6-8):(1.5-2.5):(0.7-1.3).
Description
Technical field
The present invention relates to a kind of composition and method of making the same and application, be meant a kind of composition and method of making the same and the application of said composition in the medicine of preparation protection gastric mucosa especially from Pollen Pini and Rhizoma Curcumae Longae from plant.
Background technology
Pollen Pini is Pinaceae Pinus massoniana Lamb (Pinus massoniana Lamb.), Pinus tabuliformis (Pinus tabulaeformis Carr.) or the dry pollen that belongs to the seeds plant together, it is yellow to have another name called pine, the record of " pine is yellow " is just arranged in first pharmacopeia Shennong's Herbal of China, its function is: " pine is yellow, and abnormal smells from the patient is sweet flat nontoxic, cures mainly trusted subordinate's cold and heat pathogen; diuresis; the congestion that disappears, and obeys the physical strength profiting of making light of one's life by commiting suicide for a long time, prolongs life." with its classify as top grade [Wang Min. the composition of Pollen Pini and Advance on Pharmacological Activities [J] Anhui medicine, 2008,12 (4): 357-358.].The history of Pollen Pini dietotherapeutic has exceeded thousands of years, before China utilizes the history of Pollen Pini can trace back to 2400 at least.Now be widely used in fields such as health food, medicine, cosmetics.And relevant studies show that, effects such as Pollen Pini removes can resisting fatigue, slow down aging, human body immunity improving power also have adjusting gastrointestinal function [Luo Yanxi, Cai Huafang, Zhong Yusen. medicinal efficacy progress [J] China Dispensary of Pollen Pini, 2009,20 (12): 957-958.].Contain abundant vitamin B1 in the Pollen Pini, can promote intestinal peristalsis promoting, strengthen digestive function, its rich dietary fiber particularly content of lignin is very high, in the treatment constipation, appetite stimulator, disease aspects such as prevention duodenal ulcer, hemorrhoid, ulcerative colitis, chronic atrophic gastritis also play an important role.
Rhizoma Curcumae Longae (Rhizoma Curcumae Longae) is a kind of Chinese medicine commonly used, records that it derives from the dry rhizome of Zingiberaceae curcuma Rhizoma Curcumae Longae (Curcuma longa L.) in going through edition Pharmacopoeia of the People's Republic of China.Curcumin is one of effective ingredient of Chinese crude drug Rhizoma Curcumae Longae (Rhizoma Curcumae Longae), curcumin has the function of removing blood stasis circulation of qi promoting, inducing menstruation to relieve menalgia, the tradition traditional Chinese medical science is used for the twinge of the breast side of body, amenorrhea, rheumatism shoulder arm pain, treating swelling and pain by traumatic injury, pharmacological actions widely such as antitumor, antioxidation, antiinflammatory, blood fat reducing, and toxicity very low [Zhang Hongying. Chinese medicine Advances in research on Curcuma longa Chinese medicine Advances in research on Curcuma longa [J]. special journal 2001 is cured in the Heze, 13 (4): 84-87.], more and morely be subjected to attention both domestic and external.Extraction separation obtains based on three kinds of slightly variant phenol monomers of the molecular structure of curcumin from Chinese crude drug Rhizoma Curcumae Longae (Rhizoma Curcumae Longae), be curcumin (I, curcumin), demethoxycurcumin (II, Demethoxycurcumin) and bisdemethoxycurcumin (III, Bisdemethoxycurcumin), molecular formula is respectively C
21H
20O
6, C
20H
18O
5And C
19H
16O
4, chemical structural formula is as follows:
They have the function of removing blood stasis circulation of qi promoting, inducing menstruation to relieve menalgia, the tradition traditional Chinese medical science is used for the twinge of the breast side of body, amenorrhea, rheumatism shoulder arm pain, treating swelling and pain by traumatic injury, pharmacological actions widely such as antitumor, antioxidation, antiinflammatory, blood fat reducing, and toxicity very low [Zhang Hongying. special journal 2001 is cured in Chinese medicine Advances in research on Curcuma longa Chinese medicine Advances in research on Curcuma longa [J] Heze, 13 (4): 84-87.], more and morely be subjected to attention both domestic and external.
Gastric ulcer is a kind of commonly encountered diseases, frequently-occurring disease, and easily recurrence, clinical manifestation be the periodicity upper abdominal pain, and have hemorrhage, bore a hole and complication such as block, part gastric ulcer even also canceration can take place.The western medical treatment gastric ulcer is at present main to adopt proton pump inhibitor, bisfentidine and gastric mucosa protective agent and treatment such as eliminating pylorus in case of necessity.Though this disease of western medical treatment can be faster and is made ulcer healing significantly; its short-term healing rate no longer is a problem; but 1 year relapse rate is up to 30%-50% after the drug withdrawal; and use for a long time acid inhibitor will cause problems such as dyspepsia generation [neat Jingjing. and in the research of soup of bursting that disappears to acetic acid ulcer rat model gastric mucosal protective effect. Hubei: Hubei College Of Traditional Chinese Medicine, 2009.].Therefore, seeking the effective ways for the treatment of gastric ulcer from Chinese medicine is a job that is worth expectation.
Studies show that; Pollen Pini just can help its effective ingredient of absorption of human body after having only breaking cellular wall; and very easily oxidation and microbiological contamination behind the breaking cellular wall; and Rhizoma Curcumae Longae extract removes and can have protective effect at the generation and the pair cell of ulcer surface inflammation-inhibiting reaction; itself also have antioxidation, antibiotic, blood fat reducing and antiatherogenic pharmacologically active [Bao Huaying; Chen Ronghua. the progress of curcumin [J] foreign medical science pediatrics fascicle, 2003,30 (5): 254-256.].The The combined medication is complemented each other, and can improve the antiinflammatory pharmacologically active.
Summary of the invention
The invention provides a kind of suppress to be better than aspect the gastric mucosa injury breaking cellular wall Pollen Pini or the independent medication of Rhizoma Curcumae Longae extract, protect application in the medicine of gastric mucosa from the composition and method of making the same of Pollen Pini and Rhizoma Curcumae Longae and said composition in preparation.
A kind of compositions, it comprises by mass parts:
Breaking cellular wall Pollen Pini 30-39 part
1 part of Rhizoma Curcumae Longae extract;
Wherein Rhizoma Curcumae Longae extract is (6-8) by mass ratio: (1.5-2.5): curcumin (0.7-1.3), demethoxycurcumin, bisdemethoxycurcumin are formed.
The present composition, the mass ratio of wherein said described curcumin, demethoxycurcumin, bisdemethoxycurcumin is 7: 2: 1.
The present composition, the particle diameter of wherein said breaking cellular wall Pollen Pini are 3-5 μ m.
The present composition, its optimum ratio by mass parts is:
36 parts of breaking cellular wall Pollen Pini
1 part of Rhizoma Curcumae Longae extract.
A kind of oral administration solid health product of above-mentioned composition, it can be by combining preparation with pharmaceutically acceptable solid excipient of above-mentioned composition and one or more or adjuvant by a certain percentage.
The oral administration solid health product of the present composition, the dosage form of wherein said oral administration solid health product comprises: tablet, capsule or pill.
The oral administration solid health product of the present composition, wherein said solid excipient or adjuvant comprise: microcrystalline Cellulose, starch, vegetable oil and/or pregelatinized Starch.
The application of a kind of above-mentioned composition in the medicine of preparation protection gastric mucosa.
The application of above-mentioned composition of the present invention in the medicine of preparation protection gastric mucosa, the medicine of wherein said protection gastric mucosa comprises: the medicine of treatment gastric ulcer, acute gastritis, chronic gastritis, duodenal ulcer, superficial gastritis and/or chronic atrophic gastritis.
The inhibitory action that gastric mucosa injury showed that the present invention causes ethanol, indometacin, acetic acid is better than using separately breaking cellular wall Pollen Pini or Rhizoma Curcumae Longae extract; the ulcer index of Rhizoma Curcumae Longae extract associating Pollen Pini group will be lower than Rhizoma Curcumae Longae extract group and Pollen Pini group, plays the effect of protection gastric mucosa.
Application in the medicine of preparation protection gastric mucosa is described further with said composition to the present invention and preparation method thereof below in conjunction with accompanying drawing.
Description of drawings
Fig. 1 is the stomach section of normal experimental rat among the present invention, amplification 10 * 10,
Fig. 2 is the stomach section of normal experimental rat among the present invention, amplification 40 * 10,
Fig. 3 is the stomach section of experimental rat in the model control group among the present invention, amplification 10 * 10,
Fig. 4 is the stomach section of experimental rat in the model control group among the present invention, amplification 40 * 10,
Fig. 5 is the stomach section of experimental rat in the cimetidine positive drug group among the present invention, amplification 10 * 10,
Fig. 6 is the stomach section of experimental rat in the cimetidine positive drug group among the present invention, amplification 40 * 10,
Fig. 7 is the stomach section of experimental rat in the Pollen Pini group among the present invention, amplification 10 * 10,
Fig. 8 is the stomach section of experimental rat in the Pollen Pini group among the present invention, amplification 40 * 10,
Fig. 9 is the stomach section of experimental rat in the Rhizoma Curcumae Longae extract group among the present invention, amplification 10 * 10,
Figure 10 is the stomach section of experimental rat in the Rhizoma Curcumae Longae extract group among the present invention, amplification 40 * 10,
Figure 11 is the stomach section of experimental rat in the Rhizoma Curcumae Longae extract associating Pollen Pini group among the present invention, amplification 10 * 10,
Figure 12 is the stomach section of experimental rat in the Rhizoma Curcumae Longae extract associating Pollen Pini group among the present invention, amplification 40 * 10.
The specific embodiment
Embodiment 1: the preparation of Rhizoma Curcumae Longae extract
1, dry traditional Chinese medicine material Rhizoma Curcumae Longae is pulverized the back and cross 40 mesh sieves;
2, get the powder 500g that sieves and obtain, 80% alcohol heating reflux 2h with its 8 times of amounts extracts at every turn, extracts altogether three times, extracts the after-filtration extracting solution at every turn, and merge extractive liquid;
3, concentrating under reduced pressure and drying under reduced pressure to Rhizoma Curcumae Longae extract extractum does not have the alcohol flavor, obtains Rhizoma Curcumae Longae extract extractum 40g.
4, can dissolve fully to it with acetic acid ethyl dissolution Rhizoma Curcumae Longae extract extractum 40g, add 80-120 purpose silica gel 100g, make silica gel fully adsorb Rhizoma Curcumae Longae extract, and under vacuum state, 60 ℃ of oven dry obtain silica gel mixed sample (rufous), and be standby.
5, take by weighing 1800g (200-300 order) silica gel, dry method is loaded on chromatographic column (diameter 100mm, column length 900mm) in, be the eluent eluting with pure chloroform earlier, one bottle of every 1000ml is after 20 bottles of pure chloroform eluting, (99: 1v/v) mixed liquor is the eluent eluting with chloroform-methanol again, when having bright yellow solution to flow out, begin to collect effusive solution, detect (CHCl by volume in TLC
3: CH
3OH: HCOOH=96: 4: 0.7 mixed liquor is as developing solvent), do not flow out to there being eluate.Concentrating under reduced pressure obtains Rhizoma Curcumae Longae extract.
6, the Rhizoma Curcumae Longae extract that obtains is carried out HPLC and detect, use HPLC to detect the condition of Rhizoma Curcumae Longae extract: chromatographic column: Alltima C18, particle diameter 5 μ m, column length 250mm, diameter 4.6mm; Mobile phase: 0.2% acetic acid aqueous solution-acetonitrile (40: 60); Detect wavelength: 420nm; Sample size: 20 μ l; Column temperature: 30 ℃.Be that 7: 2: 1 curcumins, demethoxycurcumin, bisdemethoxycurcumins are formed by mass ratio in the step 5 gained Rhizoma Curcumae Longae extract.
Breaking cellular wall Pollen Pini can be commercial from many places described in the embodiment 2-8, and this breaking cellular wall Pollen Pini is available from Yantai New Era Health Industry Co., Ltd., and its particle diameter is 3-5 μ m, and described Rhizoma Curcumae Longae extract is to extract among the embodiment 1.
Embodiment 2: from Pollen Pini and Rhizoma Curcumae Longae extract preparation of compositions method
Breaking cellular wall Pollen Pini and Rhizoma Curcumae Longae extract mix, and are made into suspension with 0.9% normal saline.
Embodiment 3:
Take by weighing breaking cellular wall Pollen Pini 930g, the Rhizoma Curcumae Longae extract 30g that obtains among the embodiment 1, mixing, cross 80 mesh sieves, add microcrystalline Cellulose 1033g, starch 465g and form pharmaceutical formulation as diluent, mixing, spraying into 75% ethanol is binding agent system soft material, granulates with 24 mesh sieves, dry back granulate, mixing, tabletting is made 1000, coating, that is every mixture 960mg that contains breaking cellular wall Pollen Pini and Rhizoma Curcumae Longae extract.
Embodiment 4:
Take by weighing breaking cellular wall Pollen Pini 1800g, the Rhizoma Curcumae Longae extract 50g that obtains among the embodiment 1, mixing, cross 80 mesh sieves, be suspended among the soybean oil 7400g, grind evenly through colloid mill, as feed liquid, make 1000, drying, typing, packing promptly, every mixture 1850mg that contains breaking cellular wall Pollen Pini and Rhizoma Curcumae Longae extract.
Embodiment 5:
Take by weighing breaking cellular wall Pollen Pini 390g, the Rhizoma Curcumae Longae extract 10g that obtains among the embodiment 1, mixing, cross 80 mesh sieves, in the gained powder, add pregelatinized Starch 1018g, microcrystalline Cellulose 291g forms pharmaceutical formulation, mixing as diluent, spraying into 75% ethanol is binding agent system soft material, granulates with 24 mesh sieves, dry back granulate, mixing, encapsulated, make 1000, that is every mixture 400mg that contains breaking cellular wall Pollen Pini and Rhizoma Curcumae Longae extract.
Embodiment 6: breaking cellular wall Pollen Pini and Rhizoma Curcumae Longae extract compositions cause the influence of rat gastric ulcer model to dehydrated alcohol
1. laboratory animal and group setting
50 of healthy adult cleaning level SD rats, male, body weight (220 ± 20) g.The employing posture is raised in cages, 22 ± 3 ℃ of ambient temperatures, humidity 55 ± 5%.Animal begins experiment after adaptability is raised a week.Be divided into 5 groups at random, 10 every group, conduct respectively: model control group, cimetidine positive drug control group, Pollen Pini group, Rhizoma Curcumae Longae extract group, Rhizoma Curcumae Longae extract associating Pollen Pini group.
2. dehydrated alcohol causes the rat gastric ulcer Preparation of model
Before the modeling, administration in the following manner: model control group gives normal saline; The cimetidine positive drug control group gives cimetidine by the dosage of 150mg/kg; The Pollen Pini group gives Pollen Pini by the dosage of 1800mg/kg; The Rhizoma Curcumae Longae extract group gives Rhizoma Curcumae Longae extract by the dosage of 50mg/kg; Rhizoma Curcumae Longae extract associating Pollen Pini group by the dosage of 50mg/kg give Rhizoma Curcumae Longae extract, the dosage by 1800mg/kg gives Pollen Pini simultaneously.Above-mentionedly be administration every day 1 time, gastric infusion is 14 days continuously.
Behind modeling gastric infusion the previous day, water 24h is can't help in fasting.2h behind the last gastric infusion, each rat is all irritated stomach and gives dehydrated alcohol 1ml, behind the 1h with 10% chloral hydrate anesthesia animal.Open the abdominal cavity then, abdominal aortic blood, centrifugal 15 minutes of 3500r/min gets supernatant, and it is standby to deposit in-20 ℃ of refrigerators; Ligation cardia and pylorus inject 1% formalin 8ml to gastric respectively.Take out stomach, be soaked in 1% formalin, take out behind the 20min, cut stomach open, wash coat of the stomach gently, wash out content, launch to observe the gastric mucosa injury situation, calculate gastric ulcer index and ulcer inhibition rate with normal saline along greater gastric curvature.
3. gastric ulcer index and ulcer inhibition rate standards of grading
With the streak ulcer length of vernier caliper measurement gastric mucosa (mm), every 1mm meter 1 minute, width doubles its score greater than 1mm person, point-like ulcer, 3 some meters 1 minute calculate total points.With its total points as ulcer index.[Xu Shuyun, the chief editor. pharmacological experimental methodology (the 2nd edition) [M]. Beijing: People's Health Publisher, 1991:453-456.] and calculate ulcer inhibition rate, ulcer inhibition rate=(model control group ulcer index-administration group ulcer index)/model control group ulcer index * 100%.The comparable group differences.All experimental results are with mean ± standard deviation
Expression is organized a significant difference relatively with the t check, and P＜0.05 expression difference has statistical significance.
4. experimental technique
4.1 morphological histology is observed
Stomach is soaked in 10% the formalin fixing, the routine paraffin wax embedding, pathological section is done in hematoxylin-eosin (HE) dyeing.On the painted serial section of HE, with microscopic examination gastric mucosa injury situation.
42 nitric oxide (NO) assay
(bio-engineering research institute is built up in Nanjing, lot number: the 20100321) step of description operation, absorbance is surveyed with microplate reader in the colour developing back at the 550nm place by the NO detection kit.
4.3 superoxide dismutase (SOD) vitality test
The employing xanthine oxidase carries out.Specifically undertaken by SOD detection kit (Beijing Bang Ding Biomedicines, Inc.) description.
4.4 malonaldehyde (MDA) assay
Adopt thiobarbituricacid (TBA) method, principle is MDA and TBA condensation, and the red product of formation has absorption maximum at the 532nm place, obtains every milliliter of Content of MDA (n mol/ml).Press the operation of MDA detection kit (Beijing Bang Ding Biomedicines, Inc.) description, survey absorbance in the 532nm place with microplate reader,
5. result and analysis
5.1 ethanol is caused the influence of rat gastric ulcer
Pollen Pini group, Rhizoma Curcumae Longae extract group, Rhizoma Curcumae Longae extract associating Pollen Pini group and model control group more all can suppress rat ethanol gastric mucosa injury, and wherein Rhizoma Curcumae Longae extract associating Pollen Pini group and model control group relatively suppress significantly (P＜0.05); Compare with the Rhizoma Curcumae Longae extract group with the Pollen Pini group with single, Rhizoma Curcumae Longae associating Pollen Pini group is better than the single drug group to the inhibitory action that gastric mucosa injury showed.See Table 1.
Table 1 pair ethanol cause rat pipe film injury influence (
N=10)
Compare with model control group:
*P＜0.05,
*P＜0.01
5.2NO, MDA assay and SOD determination of activity
With model control group relatively, the Pollen Pini group can significantly improve gastric ulcer rat blood serum nitric oxide (NO) content (P＜0.01) that ethanol causes, the activity (P＜0.05) of SOD, and reduces the content of MDA; The equal elevation of NO content of Rhizoma Curcumae Longae extract group, Rhizoma Curcumae Longae extract associating Pollen Pini group, but all can not the increased SOD activity, two groups all can be reduced MDA content, and Rhizoma Curcumae Longae extract associating Pollen Pini group effect is more remarkable.
Table 2 pair ethanol cause gastric ulcer rat blood serum NO, MDA and SOD influence (
N=10)
Compare with model control group:
*P＜0.05,
*P＜0.01
5.3 morphology qualitative observation
Experimental result shows: each layer of normal rat gastric tissue structural integrity, and the intact nothing in epidermis upper strata comes off, and mucomembranous gland is arranged in order, and cell is arranged closely (seeing Fig. 1 and Fig. 2); The model control group rat stomach is organized the top layer exuviation, and the mucomembranous gland normal configuration disappears, and is misaligned, the gland cell decreased number, and cell volume dwindles, especially chief cell loss serious (seeing Fig. 3 and Fig. 4); Cimetidine positive drug group gastric mucosa of rat minor injury, but only be confined to coming off of epidermis upper strata, body of gland is intact, and cell is arranged closely, and cell number does not reduce (seeing Fig. 5 and Fig. 6).
Compare with the model control group rat, Pollen Pini group gastric mucosa of rat epidermis upper strata comes off, and cell volume dwindles, but cell number does not reduce cellularity marshalling (seeing Fig. 7 and Fig. 8); Rhizoma Curcumae Longae extract group rat mucodermis upper strata comes off, and exudate is arranged down, and cell number does not reduce, but cell has damage, has been out of shape atrophy, overall structure more complete (seeing Fig. 9 and Figure 10).Rhizoma Curcumae Longae extract associating Pollen Pini group gastric mucosa of rat top layer has the kitchen range shape to come off, and it is less to come off than positive drug group, and the gland cell number just has minimizing slightly, cellular atrophy, and body of gland is arranged more neat, generally structure more intact (seeing Figure 11 and Figure 12).
From the morphology qualitative observation, tentatively as can be seen, Pollen Pini group, Rhizoma Curcumae Longae extract group have protective effect to the gastric mucosa that ethanol causes the gastric ulcer model rat.And Pollen Pini and Rhizoma Curcumae Longae extract drug combination group, it is more neat that gland cell is arranged, and the cell comparatively dense just is damaged to mucosa top, and cellular atrophy only appears in the bottom, and the cell number disappearance is also less, better effects if.
Embodiment 7: Pollen Pini and Rhizoma Curcumae Longae extract compositions cause the influence of rat gastric ulcer model to indometacin
1. laboratory animal and group setting
With embodiment 2.
2. indometacin causes the rat gastric ulcer Preparation of model
Before the modeling, administration in the following manner: model control group gives normal saline; The cimetidine positive drug control group gives cimetidine by the dosage of 150mg/kg; The Pollen Pini group gives Pollen Pini by the dosage of 1800mg/kg; The Rhizoma Curcumae Longae extract group gives Rhizoma Curcumae Longae extract by the dosage of 50mg/kg; Rhizoma Curcumae Longae extract associating Pollen Pini group by the dosage of 50mg/kg give Rhizoma Curcumae Longae extract, the dosage by 1800mg/kg gives Pollen Pini simultaneously.Above-mentionedly be administration every day 1 time, gastric infusion is 14 days continuously.
Behind the 13rd day gastric infusion, water 24h is can't help in fasting.1.5h behind the 14th day gastric infusion, each rat head subcutaneous injection indometacin 40mg/kg uses the chloral hydrate anesthesia animal behind the 5h, and the dosage of chloral hydrate is the 0.35ml/100g rat.Open the abdominal cavity then, ligation cardia and pylorus inject 1% formalin 8ml to gastric respectively.Take out stomach, be soaked in 1% formalin, take out behind the 20min, cut stomach open, wash coat of the stomach gently, clean content, launch to observe the gastric mucosal damage situation with normal saline along greater gastric curvature.
3. ulcer index determining and assessment method
Ulcer level is divided into 6 grades as ulcer index, and complete mucosa is 0 grade, and petechial hemorrhage (diameter＞1mm), the 1-5 point is 1 grade, the 6-10 point is 2 grades, is 3 grades more than 10, and the 1-5 strip is hemorrhage to be 4 grades, the 6-10 bar is 5 grades, article 10, more than be 6 grades, the hemorrhage diameter of strip＞1mm counts 1, and 1mm＜diameter≤2mm counts 2,2mm＜diameter≤3mm counts 3, by that analogy.And calculate ulcer and suppress percentage rate, ulcer inhibition rate=(model control group ulcer index-administration group ulcer index)/model control group ulcer index * 100%, comparable group differences.All experimental results are with mean ± standard deviation
Expression is organized a significant difference relatively with the t check, is that difference has statistical significance with P＜0.05.
4. result and analysis
Pollen Pini group, Pollen Pini associating Rhizoma Curcumae Longae extract group all can significantly suppress rat indometacin type gastric mucosa injury (P＜0.05, P＜0.01), compare with model control group, and Pollen Pini associating Rhizoma Curcumae Longae extract group has significant difference.See Table 3.
Table 3 Pollen Pini to indometacin cause rat pipe film injury influence (
N=10)
Compare with model control group:
*P＜0.05,
*P＜0.01
Embodiment 8: Pollen Pini and Rhizoma Curcumae Longae extract compositions cause the influence of rat gastric ulcer model to acetic acid
1. laboratory animal and group setting
With embodiment 2.
2. acetic acid causes rat gastric ulcer Preparation of model and experimental technique
Rat is adopted the modeling of acetic acid calcination method, modeling method adopts Okabe method [Okabe S, Roth JL, Pfeiffer CJ.A method for experimental, penetrating gastric and duodenal ulcers in rats[J] .Observations on normal healing.Am J Dig Dis.1971,16:277-2841.], and adjust slightly.Fasting is 24 hours before the rat modeling, freely drinks water.10% chloral hydrate is pressed the dosage lumbar injection of 3.5ml/kg, animal is anaesthetized, after animal enters narcotism, its high crouching is fixed on the big rat holder, with iodine tincture the rat abdomen fur is sterilized, drape, in the ensiform process of sternum lower edge ventrimeson about 2cm of layering open abdomen that takes back slightly, draw stomach and expose glandular stomach portion, get 100% acetic acid, 75 μ l with microsyringe, the about 5mm of diameter, the pipe of long 3cm vertically is attached on the serosal surface of body of stomach and pyloric antrum intersection, in pipe, add the acetic acid of 75 μ l, behind the 1min, dip in out acetic acid with cotton swab, the dried cotton swab of reuse is gently with the residual acetic acid exhaustion on coat of the stomach surface, and with the cotton swab that contains normal saline twice of wiping gently.Also include stomach in abdominal cavity carefully, nethike embrane in local the covering is sent into the abdominal cavity, then the layer-by-layer suture otch.Lumbar injection penicillin sodium 0.2ml, in case wound infection, anesthetized rat is all revived in 2-3h, postoperative is given the continuous lumbar injection penicillin sodium of rat 3 days.
Administration in the following manner: model control group gives normal saline; The cimetidine positive drug control group gives cimetidine by the dosage of 150mg/kg; The Pollen Pini group gives Pollen Pini by the dosage of 1800mg/kg; The Rhizoma Curcumae Longae extract group gives Rhizoma Curcumae Longae extract by the dosage of 50mg/kg; Rhizoma Curcumae Longae extract associating Pollen Pini group by the dosage of 50mg/kg give Rhizoma Curcumae Longae extract, the dosage by 1800mg/kg gives Pollen Pini simultaneously.
Beginning gastric infusion, administration in the following manner in the 5th day after the modeling: model control group gives normal saline; The cimetidine positive drug control group gives cimetidine by the dosage of 150mg/kg; The Pollen Pini group gives Pollen Pini by the dosage of 1800mg/kg; The Rhizoma Curcumae Longae extract group gives Rhizoma Curcumae Longae extract by the dosage of 50mg/kg; Rhizoma Curcumae Longae extract associating Pollen Pini group by the dosage of 50mg/kg give Rhizoma Curcumae Longae extract, the dosage by 1800mg/kg gives Pollen Pini simultaneously.Every day 1 time, successive administration 14 days.Water 24h is can't help in fasting after the last administration, the chloral hydrate anesthesia animal.Open the abdominal cavity, ligation cardia and pylorus inject 1% formalin 8ml to gastric respectively.Take out stomach, be soaked in 1% formalin, take out stomach behind the 20min, cut stomach open, wash coat of the stomach gently, launch to observe the gastric mucosa injury situation with normal saline along greater gastric curvature.
Ulcer index determining and assessment method [Zhang Shouren. furazolidone and some medicines commonly used are to the influence of four kinds of gastric ulcer models. Acta Pharmaceutica Sinica, 1984,19 (1) 5-12.]
Go out the major diameter and the wideest footpath of ulcer with vernier caliper measurement, both multiply each other note as ulcer index.And the calculating ulcer inhibition rate, ulcer inhibition rate=(model control group ulcer index-administration group ulcer index)/model control group ulcer index * 100%, comparable group differences.All experimental results are with mean ± standard deviation
Expression is organized a significant difference relatively with the t check, is that difference has statistical significance with P＜0.05.
4. result and analysis
The Pollen Pini group, Rhizoma Curcumae Longae extract group and Pollen Pini associating Rhizoma Curcumae Longae extract group all can suppress rats acetic acid type gastric mucosa injury, compare with model control group, Rhizoma Curcumae Longae extract group and Pollen Pini associating Rhizoma Curcumae Longae extract group all have significant difference, and wherein the effect of Pollen Pini associating Rhizoma Curcumae Longae extract group is more obvious.See Table 4.
Table 4 Pollen Pini to the ulcer index of acetic acid-induction gastric mucosa injury and ulcer inhibition rate (
N=10)
Compare with model control group:
*P＜0.05,
*P＜0.01
Above-described embodiment is described preferred implementation of the present invention; be not that scope of the present invention is limited; design under the prerequisite of spirit not breaking away from the present invention; various distortion and improvement that those of ordinary skills make technical scheme of the present invention all should fall in the definite protection domain of claims of the present invention.
Claims (9)
1. compositions is characterized in that: comprise the following component by mass parts:
Breaking cellular wall Pollen Pini 30-39 part
1 part of Rhizoma Curcumae Longae extract;
Wherein Rhizoma Curcumae Longae extract is (6-8) by mass ratio: (1.5-2.5): curcumin (0.7-1.3), demethoxycurcumin, bisdemethoxycurcumin are formed.
2. compositions according to claim 1 is characterized in that: the mass ratio of described curcumin, demethoxycurcumin, bisdemethoxycurcumin is 7: 2: 1.
3. compositions according to claim 1 and 2 is characterized in that: the particle diameter of described breaking cellular wall Pollen Pini is 3-5 μ m.
4. compositions according to claim 3 is characterized in that: described optimum ratio by mass parts is:
36 parts of breaking cellular wall Pollen Pini
1 part of Rhizoma Curcumae Longae extract.
5. the oral administration solid health product of an above-mentioned composition is characterized in that: can be by pharmaceutically acceptable solid excipient of above-mentioned composition and one or more or adjuvant are combined preparation by a certain percentage.
6. the oral administration solid health product of compositions according to claim 5, it is characterized in that: the dosage form of described oral administration solid health product comprises: tablet, capsule or pill.
7. according to the oral administration solid health product of claim 5 or 6 described compositionss, it is characterized in that: described solid excipient or adjuvant comprise: microcrystalline Cellulose, starch, vegetable oil and/or pregelatinized Starch.
8. the application of above-mentioned composition in the medicine of preparation protection gastric mucosa.
9. the application of compositions according to claim 8 in the medicine of preparation protection gastric mucosa, it is characterized in that: the medicine of described protection gastric mucosa comprises: the medicine of treatment gastric ulcer, acute gastritis, chronic gastritis, duodenal ulcer, superficial gastritis and/or chronic atrophic gastritis.
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| CN201110177962.0A CN102228665B (en) | 2011-06-29 | 2011-06-29 | Composition prepared from pine pollen and curcuma, preparation method thereof, and application of composition in preparing medicament for protecting gastric mucosa |
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| CN1309997A (en) * | 2001-02-14 | 2001-08-29 | 金光善 | Hualu health article and its preparation |
| CN1931353A (en) * | 2006-09-27 | 2007-03-21 | 贵州师范大学 | Curcuma extract and its prepn process, medicine composition and use |
| CN101444543A (en) * | 2007-11-28 | 2009-06-03 | 仪徐生 | Pine pollen stomach-nourishing tablet |
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2011
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|---|---|---|---|---|
| CN1309997A (en) * | 2001-02-14 | 2001-08-29 | 金光善 | Hualu health article and its preparation |
| CN1931353A (en) * | 2006-09-27 | 2007-03-21 | 贵州师范大学 | Curcuma extract and its prepn process, medicine composition and use |
| CN101444543A (en) * | 2007-11-28 | 2009-06-03 | 仪徐生 | Pine pollen stomach-nourishing tablet |
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| 《中南林业科技大学学报》 20090630 李湘洲等 姜黄色素的生物活性和提取分离研究进展 具体参见第190页第1-6行，第192页第3-5，14-19行 5，8 第29卷, 第3期 * |
| 李湘洲等: "姜黄色素的生物活性和提取分离研究进展", 《中南林业科技大学学报》, vol. 29, no. 3, 30 June 2009 (2009-06-30) * |
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| CN102228665B (en) | 2014-04-30 |
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