CN102228665B - Composition prepared from pine pollen and curcuma, preparation method thereof, and application of composition in preparing medicament for protecting gastric mucosa - Google Patents
Composition prepared from pine pollen and curcuma, preparation method thereof, and application of composition in preparing medicament for protecting gastric mucosa Download PDFInfo
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Abstract
The invention provides a composition prepared from pine pollen and curcuma, a preparation method thereof, and application of the composition in preparing a medicament for protecting the gastric mucosa, relating to a composition prepared from plants and a preparation method and application thereof. The composition provided by the invention can alleviate gastric ulcer caused by ethanol, indomethacin, acetic acid and the like, and can be used for preparing a medicament for protecting the gastric mucosa. The composition comprises the following components in parts by weight: 30-39 parts of wall-broken pine pollen and 1 part of curcuma extract, wherein the curcuma extract is composed of curcumin, demethoxycurcumin and bisdemethoxycurcumin in mass ratio of (6-8):(1.5-2.5):(0.7-1.3).
Description
Technical field
The present invention relates to a kind of composition and method of making the same from plant and application, refer to especially the application in the medicine of preparation protection gastric mucosa of a kind of composition and method of making the same from Pollen Pini and Rhizoma Curcumae Longae and said composition.
Background technology
Pollen Pini is Pinaceae Pinus massoniana Lamb (Pinus massoniana Lamb.), Pinus tabuliformis (Pinus tabulaeformis Carr.) or the dry pollen that belongs to seeds plant together, have another name called pine yellow, in China First pharmacopeia < < Sheng Nong's herbal classic > >, just there is the record of " pine is yellow ", its function is: " pine is yellow; abnormal smells from the patient is sweet flat nontoxic; cure mainly trusted subordinate's cold and heat pathogen; diuresis; congestion disappears; take for a long time the physical strength profiting of making light of one's life by commiting suicide, prolong life." classified as top grade [Wang Min. the composition of Pollen Pini and Advance on Pharmacological Activities [J] Anhui medicine, 2008,12 (4): 357-358.].The history of Pollen Pini dietotherapeutic has exceeded thousands of years, and China utilizes the history of Pollen Pini at least can trace back to before 2400.Now be widely used in the fields such as health food, medicine, cosmetics.And relevant research shows, Pollen Pini except can resisting fatigue, the effect such as slow down aging, raising immunity of organisms, also there is adjusting gastrointestinal function [Luo Yanxi, Cai Huafang, Zhong Yusen. medicinal efficacy progress [J] China Dispensary of Pollen Pini, 2009,20 (12): 957-958.].In Pollen Pini, contain abundant vitamin B1, can promote intestinal peristalsis promoting, strengthen digestive function, its abundant dietary fiber particularly content of lignin is very high, in treatment constipation, appetite stimulator, the disease aspects such as prevention duodenal ulcer, hemorrhoid, ulcerative colitis, chronic atrophic gastritis also play an important role.
Rhizoma Curcumae Longae (Rhizoma Curcumae Longae) is a kind of conventional Chinese medicine, records that it derives from the dry rhizome of Zingiberaceae curcuma Rhizoma Curcumae Longae (Curcuma longa L.) in going through edition < < Pharmacopoeia of People's Republic of China > >.Curcumin is one of effective ingredient of Chinese crude drug Rhizoma Curcumae Longae (Rhizoma Curcumae Longae), curcumin has the function of removing blood stasis circulation of qi promoting, inducing menstruation to relieve menalgia, Traditional Chinese Medicine is for the twinge of the breast side of body, amenorrhea, rheumatism shoulder arm pain, treating swelling and pain by traumatic injury, the pharmacological actions widely such as antitumor, antioxidation, antiinflammatory, blood fat reducing, and toxicity very low [Zhang Hongying. the progress [J] of the progress Turmeric of Turmeric. special journal 2001 is cured in Heze, 13 (4): 84-87.], get more and more and be subject to attention both domestic and external.From Chinese crude drug Rhizoma Curcumae Longae (Rhizoma Curcumae Longae), extract to separate and obtain take curcumin as the main slightly discrepant three kinds of phenol monomers of molecular structure, be curcumin (I, curcumin), demethoxycurcumin (II, Demethoxycurcumin) and bisdemethoxycurcumin (III, Bisdemethoxycurcumin), molecular formula is respectively C
21h
20o
6, C
20h
18o
5and C
19h
16o
4, chemical structural formula is as follows:
They have the function of removing blood stasis circulation of qi promoting, inducing menstruation to relieve menalgia, Traditional Chinese Medicine is for the twinge of the breast side of body, amenorrhea, rheumatism shoulder arm pain, treating swelling and pain by traumatic injury, the pharmacological actions widely such as antitumor, antioxidation, antiinflammatory, blood fat reducing, and toxicity very low [Zhang Hongying. special journal 2001 is cured in progress [J] Heze of the progress Turmeric of Turmeric, 13 (4): 84-87.], get more and more and be subject to attention both domestic and external.
Gastric ulcer is a kind of commonly encountered diseases, frequently-occurring disease, and easily recurrence, clinical manifestation be periodicity upper abdominal pain, and may have hemorrhage, bore a hole and the complication such as block, even also can there is canceration in part gastric ulcer.The treatments such as the current main employing proton pump inhibitor of western medical treatment gastric ulcer, bisfentidine and gastric mucosa protective agent and if desired eliminating pylorus.Although this disease of western medical treatment can faster and significantly make ulcer healing; its short-term healing rate is no longer problem; but after drug withdrawal, 1 year relapse rate is up to 30%-50%; and use for a long time acid inhibitor by cause the problems such as dyspepsia generation [neat Jingjing. and in the research of soup to the protective effect of acetic acid Ulcer Models gastric mucosa of rat of bursting that disappears. Hubei: Hubei College Of Traditional Chinese Medicine, 2009.].Therefore, from Chinese medicine, the effective ways of searching treatment gastric ulcer are the work of a Worth Expecting.
Research shows; Pollen Pini just can be conducive to its effective ingredient of absorption of human body after only having breaking cellular wall; and very easily oxidation and microbiological contamination after breaking cellular wall; and Rhizoma Curcumae Longae extract is except can and having protective effect to cell in the generation of ulcer surface inflammation-inhibiting reaction; itself also there is antioxidation, antibacterial, blood fat reducing and antiatherogenic pharmacologically active [Bao Huaying; Chen Ronghua. progress [J] the foreign medical science pediatrics fascicle of curcumin, 2003,30 (5): 254-256.].Both drug combinations, complement each other, and can improve antiinflammatory pharmacologically active.
Summary of the invention
The invention provides a kind of suppress to be better than aspect gastric mucosa injury breaking cellular wall Pollen Pini or the independent medication of Rhizoma Curcumae Longae extract, from the composition and method of making the same of Pollen Pini and Rhizoma Curcumae Longae and said composition, in preparation, protect the application in the medicine of gastric mucosa.
A kind of compositions, it comprises by mass parts:
Breaking cellular wall Pollen Pini 30-39 part
1 part of Rhizoma Curcumae Longae extract;
Wherein Rhizoma Curcumae Longae extract is (6-8) by mass ratio: (1.5-2.5): curcumin (0.7-1.3), demethoxycurcumin, bisdemethoxycurcumin form.
The present composition, the mass ratio of wherein said described curcumin, demethoxycurcumin, bisdemethoxycurcumin is 7: 2: 1.
The present composition, the particle diameter of wherein said breaking cellular wall Pollen Pini is 3-5 μ m.
The present composition, it by the optimum ratio of mass parts is:
36 parts of breaking cellular wall Pollen Pini
1 part of Rhizoma Curcumae Longae extract.
Oral administration solid health product for above-mentioned composition, it can be by being combined pharmaceutically acceptable to above-mentioned composition and one or more solid excipient or adjuvant preparation by a certain percentage.
The oral administration solid health product of the present composition, the dosage form of wherein said oral administration solid health product comprises: tablet, capsule or pill.
The oral administration solid health product of the present composition, wherein said solid excipient or adjuvant comprise: microcrystalline Cellulose, starch, vegetable oil and/or pregelatinized Starch.
The application of a kind of above-mentioned composition in the medicine of preparation protection gastric mucosa.
The application of above-mentioned composition of the present invention in the medicine of preparation protection gastric mucosa, the medicine of wherein said protection gastric mucosa comprises: the medicine for the treatment of gastric ulcer, acute gastritis, chronic gastritis, duodenal ulcer, superficial gastritis and/or chronic atrophic gastritis.
The inhibitory action that the gastric mucosa injury that the present invention causes ethanol, indometacin, acetic acid shows is better than using separately breaking cellular wall Pollen Pini or Rhizoma Curcumae Longae extract; the ulcer index of Rhizoma Curcumae Longae extract associating Pollen Pini group will be lower than Rhizoma Curcumae Longae extract group and Pollen Pini group, plays the effect of protection gastric mucosa.
Below in conjunction with accompanying drawing, to the present invention and preparation method thereof, the application in the medicine of preparation protection gastric mucosa is described further with said composition.
Accompanying drawing explanation
Fig. 1 is the stomach section of normal experimental rat in the present invention, amplification 10 × 10,
Fig. 2 is the stomach section of normal experimental rat in the present invention, amplification 40 × 10,
Fig. 3 is the stomach of experimental rat section in model control group in the present invention, amplification 10 × 10,
Fig. 4 is the stomach of experimental rat section in model control group in the present invention, amplification 40 × 10,
Fig. 5 is the stomach of experimental rat section in cimetidine positive drug group in the present invention, amplification 10 × 10,
Fig. 6 is the stomach of experimental rat section in cimetidine positive drug group in the present invention, amplification 40 × 10,
Fig. 7 is the stomach of experimental rat section in Pollen Pini group in the present invention, amplification 10 × 10,
Fig. 8 is the stomach of experimental rat section in Pollen Pini group in the present invention, amplification 40 × 10,
Fig. 9 is the stomach of experimental rat section in Rhizoma Curcumae Longae extract group in the present invention, amplification 10 × 10,
Figure 10 is the stomach of experimental rat section in Rhizoma Curcumae Longae extract group in the present invention, amplification 40 × 10,
Figure 11 is the stomach section of experimental rat in Rhizoma Curcumae Longae extract associating Pollen Pini group in the present invention, amplification 10 × 10,
Figure 12 is the stomach section of experimental rat in Rhizoma Curcumae Longae extract associating Pollen Pini group in the present invention, amplification 40 × 10.
The specific embodiment
Embodiment 1: the preparation of Rhizoma Curcumae Longae extract
1, dry Chinese crude drug Rhizoma Curcumae Longae is pulverized to rear 40 mesh sieves of crossing;
2, get the powder 500g that sieves and obtain, with 80% alcohol heating reflux 2h of its 8 times of amounts, extract at every turn, extract altogether three times, after each extraction, filter extracting solution, and merge extractive liquid;
3, concentrating under reduced pressure drying under reduced pressure, to Rhizoma Curcumae Longae extract extractum without alcohol taste, obtain Rhizoma Curcumae Longae extract extractum 40g.
4, with acetic acid ethyl dissolution Rhizoma Curcumae Longae extract extractum 40g, to it, can dissolve completely, add 80-120 object silica gel 100g, make silica gel fully adsorb Rhizoma Curcumae Longae extract, and under vacuum state, 60 ℃ of oven dry, obtain silica gel mixed sample (rufous), standby.
5, take 1800g (200-300 order) silica gel, dry method is loaded on chromatographic column (diameter 100mm, column length 900mm) in, with pure chloroform, be first eluent eluting, mono-bottle of every 1000ml, after 20 bottles of pure chloroform eluting, again take chloroform-methanol (99: 1v/v) mixed liquor as eluent eluting, from have bright yellow solution flow out time, start collect flow out solution, in TLC detect (CHCl by volume
3: CH
3oH: HCOOH=96: the mixed liquor of 4: 0.7 is as developing solvent), to flowing out without eluate.Concentrating under reduced pressure, obtains Rhizoma Curcumae Longae extract.
6, the Rhizoma Curcumae Longae extract obtaining is carried out to HPLC detection, use HPLC to detect the condition of Rhizoma Curcumae Longae extract: chromatographic column: Alltima C18, particle diameter 5 μ m, column length 250mm, diameter 4.6mm; Mobile phase: 0.2% acetic acid aqueous solution-acetonitrile (40: 60); Detect wavelength: 420nm; Sample size: 20 μ l; Column temperature: 30 ℃.In step 5 gained Rhizoma Curcumae Longae extract, by mass ratio, be that 7: 2: 1 curcumins, demethoxycurcumin, bisdemethoxycurcumins form.
Described in embodiment 2-8, breaking cellular wall Pollen Pini can be commercial from many places, and this breaking cellular wall Pollen Pini is purchased from Yantai New Era Health Industry Co., Ltd., and its particle diameter is 3-5 μ m, and described Rhizoma Curcumae Longae extract is to extract in embodiment 1.
Embodiment 2: from the preparation method of Pollen Pini and Rhizoma Curcumae Longae extract compositions
Breaking cellular wall Pollen Pini and Rhizoma Curcumae Longae extract mix, and are made into suspension with 0.9% normal saline.
Embodiment 3:
Take breaking cellular wall Pollen Pini 930g, the Rhizoma Curcumae Longae extract 30g obtaining in embodiment 1, mixes, cross 80 mesh sieves, add microcrystalline Cellulose 1033g, starch 465g to form pharmaceutical formulation as diluent, mix, spraying into 75% ethanol is binding agent soft material processed, with 24 mesh sieves, granulate, and granulate after being dried, mix, tabletting, makes 1000, coating, obtain the every mixture 960mg containing breaking cellular wall Pollen Pini and Rhizoma Curcumae Longae extract.
Embodiment 4:
Take breaking cellular wall Pollen Pini 1800g, the Rhizoma Curcumae Longae extract 50g obtaining in embodiment 1, mix, cross 80 mesh sieves, be suspended in soybean oil 7400g, through colloid mill, grind evenly, as feed liquid, make 1000, drying, sizing, packing and get final product, the every mixture 1850mg containing breaking cellular wall Pollen Pini and Rhizoma Curcumae Longae extract.
Embodiment 5:
Take breaking cellular wall Pollen Pini 390g, the Rhizoma Curcumae Longae extract 10g obtaining in embodiment 1, mixes, cross 80 mesh sieves, in gained powder, add pregelatinized Starch 1018g, microcrystalline Cellulose 291g, as diluent composition pharmaceutical formulation, mixes, spraying into 75% ethanol is binding agent soft material processed, with 24 mesh sieves, granulate, and granulate after being dried, mix, encapsulated, make 1000, obtain every mixture 400mg containing breaking cellular wall Pollen Pini and Rhizoma Curcumae Longae extract.
Embodiment 6: breaking cellular wall Pollen Pini and Rhizoma Curcumae Longae extract compositions cause the impact of rat gastric ulcer model on dehydrated alcohol
1. laboratory animal and group setting
50 of the clean level of healthy adult SD rats, male, body weight (220 ± 20) g.Employing posture is raised in cages, 22 ± 3 ℃ of ambient temperatures, humidity 55 ± 5%.Animal starts experiment after adaptability is raised one week.Be divided at random 5 groups, 10 every group, conduct respectively: model control group, cimetidine positive drug control group, Pollen Pini group, Rhizoma Curcumae Longae extract group, Rhizoma Curcumae Longae extract associating Pollen Pini group.
2. dehydrated alcohol causes the preparation of rat gastric ulcer model
Before modeling, administration in the following manner: model control group gives normal saline; Cimetidine positive drug control group gives cimetidine by the dosage of 150mg/kg; Pollen Pini group gives Pollen Pini by the dosage of 1800mg/kg; Rhizoma Curcumae Longae extract group gives Rhizoma Curcumae Longae extract by the dosage of 50mg/kg; Rhizoma Curcumae Longae extract associating Pollen Pini group gives Rhizoma Curcumae Longae extract, by the dosage of 1800mg/kg, gives Pollen Pini simultaneously by the dosage of 50mg/kg.Above-mentionedly be administration every day 1 time, continuously gastric infusion 14 days.
After modeling gastric infusion the previous day, water 24h is can't help in fasting.2h after last gastric infusion, the equal gavage of each rat gives dehydrated alcohol 1ml, after 1h with 10% chloral hydrate anesthesia animal.Then open abdominal cavity, abdominal aortic blood, centrifugal 15 minutes of 3500r/min, gets supernatant, deposits in-20 ℃ of refrigerators standby; Ligation cardia and pylorus, inject 1% formalin 8ml to gastric respectively.Take out stomach, be soaked in 1% formalin, after 20min, take out, along greater gastric curvature, cut stomach open, rinse gently coat of the stomach with normal saline, wash out content, launch to observe gastric mucosa injury situation, calculate ulcer index and ulcer inhibition rate.
3. ulcer index and ulcer inhibition rate standards of grading
By the streak ulcer length of vernier caliper measurement gastric mucosa (mm), 1 point of every 1mm meter, width is greater than 1mm person doubles its score, point-like ulcer, 3 some meters 1 minute, calculate total score.Using its total score as ulcer index.[Xu Shuyun, chief editor. pharmacological experimental methodology (the 2nd edition) [M]. Beijing: People's Health Publisher, 1991:453-456.] and calculate ulcer inhibition rate, ulcer inhibition rate=(model control group ulcer index-administration group ulcer index)/model control group ulcer index × 100%.Relatively group difference.All experimental results are with mean ± standard deviation
represent, significant difference comparison between organizing with t check, P < 0.05 represents that difference has statistical significance.
4. experimental technique
4.1 morphological histology are observed
Stomach is soaked in 10% formalin fixing, routine paraffin wax embedding, hematoxylin-eosin (HE) dyeing, does pathological section.In the serial section of HE dyeing, by microscopic examination gastric mucosa injury situation.
42 nitric oxide (NO) assay
By NO detection kit, (Bioengineering Research Institute is built up in Nanjing, lot number: the 20100321) step of description operation, the rear absorbance of surveying at 550nm place by microplate reader of colour developing.
4.3 superoxide dismutase (SOD) vitality test
Employing xanthine oxidase carries out.Specifically by SOD detection kit (Beijing Bang Ding Biomedicines, Inc.) description, undertaken.
4.4 malonaldehyde (MDA) assay
Adopt thiobarbituricacidα-(TBA) method, principle is MDA and TBA condensation, and the red product of formation has absorption maximum at 532nm place, obtains every milliliter of Content of MDA (n mol/ml).Press the operation of MDA detection kit (Beijing Bang Ding Biomedicines, Inc.) description, by microplate reader, in 532nm place, survey absorbance,
5. results and analysis
5.1 ethanol is caused to the impact of rat gastric ulcer
Pollen Pini group, Rhizoma Curcumae Longae extract group, Rhizoma Curcumae Longae extract associating Pollen Pini group and model control group more all can suppress Ethanol-Induced Gastric Mucosal Lesion in Rats, and wherein Rhizoma Curcumae Longae extract associating Pollen Pini group and model control group relatively suppress significantly (P < 0.05); Compare with Rhizoma Curcumae Longae extract group with alone Pollen Pini group, the inhibitory action that Rhizoma Curcumae Longae associating Pollen Pini group shows gastric mucosa injury is better than single drug group.In Table 1.
Table 1 on ethanol cause rat pipe film injury impact (
n=10)
With model control group comparison:
*p < 0.05,
*p < 0.01
5.2NO, MDA assay and SOD determination of activity
With model control group comparison, Pollen Pini group can significantly improve Gastric Ulcer Rats serum levels of nitric oxide (NO) content (P < 0.01) that ethanol causes, the activity (P < 0.05) of SOD, and reduces the content of MDA; The equal elevation of NO content of Rhizoma Curcumae Longae extract group, Rhizoma Curcumae Longae extract associating Pollen Pini group, but all can not increased SOD activity, two groups all can be reduced MDA content, and Rhizoma Curcumae Longae extract associating Pollen Pini group effect is more remarkable.
The impact of table 2 on ethanol Ulceration rat blood serum NO, MDA and SOD (
n=10)
With model control group comparison:
*p < 0.05,
*p < 0.01
5.3 morphology qualitative observations
Experimental result shows: each layer of structural integrity of normal rat gastric tissue, and the intact nothing in epidermis upper strata comes off, and mucomembranous gland is arranged in order, and cell arrangement is (being shown in Fig. 1 and Fig. 2) closely; Model control group rat stomach is organized top layer exuviation, and mucomembranous gland normal configuration disappears, misaligned, gland cell decreased number, and cell volume dwindles, especially chief cell loss serious (seeing Fig. 3 and Fig. 4); Cimetidine positive drug group gastric mucosa of rat minor injury, but be only confined to show peridermal coming off, body of gland is intact, and cell arrangement is tight, and cell number does not reduce (seeing Fig. 5 and Fig. 6).
With the comparison of model control group rat, Pollen Pini group gastric mucosa of rat epidermis upper strata comes off, and cell volume dwindles, but cell number do not reduce, cellularity marshalling (seeing Fig. 7 and Fig. 8); Rhizoma Curcumae Longae extract group rat mucodermis upper strata comes off, under have exudate, cell number does not reduce, but cell has damage, has been out of shape atrophy, overall structure more complete (seeing Fig. 9 and Figure 10).Rhizoma Curcumae Longae extract associating Pollen Pini group gastric mucosa of rat top layer has kitchen range shape to come off, and comes off less than positive drug group, and gland cell number just slightly reduces, cellular atrophy, and body of gland is arranged more neat, structure more intact (seeing Figure 11 and Figure 12) generally.
From morphology qualitative observation, tentatively can find out, Pollen Pini group, Rhizoma Curcumae Longae extract group have protective effect to the gastric mucosa of ethanol Ulceration rat model.And Pollen Pini and Rhizoma Curcumae Longae extract drug combination group, it is more neat that gland cell is arranged, and cell comparatively dense, is just damaged to mucosa top, and only there is cellular atrophy in bottom, and cell number disappearance is also less, better effects if.
Embodiment 7: Pollen Pini and Rhizoma Curcumae Longae extract compositions cause the impact of rat gastric ulcer model on indometacin
1. laboratory animal and group setting
With embodiment 2.
2. indometacin causes the preparation of rat gastric ulcer model
Before modeling, administration in the following manner: model control group gives normal saline; Cimetidine positive drug control group gives cimetidine by the dosage of 150mg/kg; Pollen Pini group gives Pollen Pini by the dosage of 1800mg/kg; Rhizoma Curcumae Longae extract group gives Rhizoma Curcumae Longae extract by the dosage of 50mg/kg; Rhizoma Curcumae Longae extract associating Pollen Pini group gives Rhizoma Curcumae Longae extract, by the dosage of 1800mg/kg, gives Pollen Pini simultaneously by the dosage of 50mg/kg.Above-mentionedly be administration every day 1 time, continuously gastric infusion 14 days.
After the 13rd day gastric infusion, water 24h is can't help in fasting.1.5h after the 14th day gastric infusion, each rat head subcutaneous injection indometacin 40mg/kg, uses chloral hydrate anesthesia animal after 5h, and the dosage of chloral hydrate is 0.35ml/100g rat.Then open abdominal cavity, ligation cardia and pylorus, inject 1% formalin 8ml to gastric respectively.Take out stomach, be soaked in 1% formalin, after 20min, take out, along greater gastric curvature, cut stomach open, rinse gently coat of the stomach with normal saline, clean content, launch to observe the degree of impairment of gastric mucosa.
3. ulcer index determining and assessment method
Ulcer level is divided into 6 grades as ulcer index, and complete mucosa is 0 grade, petechial hemorrhage (diameter > 1mm), 1-5 point is 1 grade, 6-10 point is 2 grades, and 10 are 3 grades above, and 1-5 strip is hemorrhage is 4 grades, 6-10 bar is 5 grades, article 10, be above 6 grades, 1 of the hemorrhage diameter > of strip 1mm meter, 2 of 1mm < diameter≤2mm meters, 3 of 2mm < diameter≤3mm meters, by that analogy.And calculate ulcer inhibition percentage, ulcer inhibition rate=(model control group ulcer index-administration group ulcer index)/model control group ulcer index × 100%, relatively group difference.All experimental results are with mean ± standard deviation
represent, significant difference comparison between organizing with t check, has statistical significance take P < 0.05 as difference.
4. results and analysis
Pollen Pini group, Pollen Pini associating Rhizoma Curcumae Longae extract group all can significantly suppress rat indometacin type gastric mucosa injury (P < 0.05, P < 0.01), compared with model control group, Pollen Pini associating Rhizoma Curcumae Longae extract group has significant difference.In Table 3.
Table 3 Pollen Pini on indometacin cause rat pipe film injury impact (
n=10)
With model control group comparison:
*p < 0.05,
*p < 0.01
Embodiment 8: Pollen Pini and Rhizoma Curcumae Longae extract compositions cause the impact of rat gastric ulcer model on acetic acid
1. laboratory animal and group setting
With embodiment 2.
2. acetic acid causes preparation and the experimental technique of rat gastric ulcer model
Rat adopts the modeling of acetic acid calcination method, modeling method adopts Okabe method [Okabe S, Roth JL, Pfeiffer CJ.A method for experimental, penetrating gastric and duodenal ulcers in rats[J] .Observations on normal healing.Am J Dig Dis.1971,16:277-2841.], and slightly adjust.Before rat modeling, fasting 24 hours, freely drinks water.10% chloral hydrate is pressed the dosage lumbar injection of 3.5ml/kg, animal is anaesthetized, animal enters after narcotism, held high sleeping being fixed on rat fixator, rat abdomen fur is sterilized with iodine tincture, drape, under ensiform process of sternum along the about 2cm of ventrimeson layering open abdomen slightly to the left, draw stomach and expose glandular stomach portion, get 100% acetic acid 75 μ l with microsyringe, by diameter 5mm, the pipe of long 3cm is vertically attached on the serosal surface of body of stomach and pyloric antrum intersection, to the acetic acid that adds 75 μ l in pipe, after 1min, dip in out acetic acid with cotton swab, with dry cotton swab, gently the residual acetic acid on coat of the stomach surface is exhausted again, and with containing twice of the wiping gently of the cotton swab of normal saline.Also include carefully stomach in abdominal cavity, nethike embrane in local covering, sends into abdominal cavity, then layer-by-layer suture otch.Lumbar injection penicillin sodium 0.2ml, in case wound infection, anesthetized rat is all revived in 2-3h, the postoperative continuous lumbar injection penicillin sodium of rat 3 days of giving.
Administration in the following manner: model control group gives normal saline; Cimetidine positive drug control group gives cimetidine by the dosage of 150mg/kg; Pollen Pini group gives Pollen Pini by the dosage of 1800mg/kg; Rhizoma Curcumae Longae extract group gives Rhizoma Curcumae Longae extract by the dosage of 50mg/kg; Rhizoma Curcumae Longae extract associating Pollen Pini group gives Rhizoma Curcumae Longae extract, by the dosage of 1800mg/kg, gives Pollen Pini simultaneously by the dosage of 50mg/kg.
After modeling the 5th day starts gastric infusion, in the following manner administration: model control group gives normal saline; Cimetidine positive drug control group gives cimetidine by the dosage of 150mg/kg; Pollen Pini group gives Pollen Pini by the dosage of 1800mg/kg; Rhizoma Curcumae Longae extract group gives Rhizoma Curcumae Longae extract by the dosage of 50mg/kg; Rhizoma Curcumae Longae extract associating Pollen Pini group gives Rhizoma Curcumae Longae extract, by the dosage of 1800mg/kg, gives Pollen Pini simultaneously by the dosage of 50mg/kg.Every day 1 time, successive administration 14 days.After last administration, water 24h is can't help in fasting, chloral hydrate anesthesia animal.Open abdominal cavity, ligation cardia and pylorus, inject 1% formalin 8ml to gastric respectively.Take out stomach, be soaked in 1% formalin, after 20min, take out stomach, along greater gastric curvature, cut stomach open, rinse gently coat of the stomach with normal saline, launch to observe gastric mucosa injury situation.
Ulcer index determining and assessment method [Zhang Shouren. the impact of furazolidone and four kinds of gastric ulcer models of some herbal pairs. Acta Pharmaceutica Sinica, 1984,19 (1) 5-12.]
The major diameter and the widest footpath that with vernier caliper measurement, go out ulcer, both multiply each other and are denoted as ulcer index.And calculate ulcer inhibition rate, ulcer inhibition rate=(model control group ulcer index-administration group ulcer index)/model control group ulcer index × 100%, relatively group difference.All experimental results are with mean ± standard deviation
represent, significant difference comparison between organizing with t check, has statistical significance take P < 0.05 as difference.
4. results and analysis
Pollen Pini group, Rhizoma Curcumae Longae extract group and Pollen Pini associating Rhizoma Curcumae Longae extract group all can suppress rats acetic acid type gastric mucosa injury, compared with model control group, Rhizoma Curcumae Longae extract group and Pollen Pini associating Rhizoma Curcumae Longae extract group all have significant difference, and wherein the effect of Pollen Pini associating Rhizoma Curcumae Longae extract group is more obvious.In Table 4.
The ulcer index of table 4 Pollen Pini to acetic acid-induction gastric mucosa injury and ulcer inhibition rate (
n=10)
With model control group comparison:
*p < 0.05,
*p < 0.01
Above-described embodiment is described the preferred embodiment of the present invention; not scope of the present invention is limited; design under the prerequisite of spirit not departing from the present invention; various distortion and improvement that those of ordinary skills make technical scheme of the present invention, all should fall in the definite protection domain of the claims in the present invention book.
Claims (8)
1. a compositions of protecting gastric mucosa, is characterized in that: the following component by mass parts, consist of:
Breaking cellular wall Pollen Pini 30-39 part
1 part of Rhizoma Curcumae Longae extract;
Wherein Rhizoma Curcumae Longae extract is (6-8) by mass ratio: (1.5-2.5): curcumin (0.7-1.3), demethoxycurcumin, bisdemethoxycurcumin form.
2. the compositions of protecting according to claim 1 gastric mucosa, is characterized in that: the mass ratio of described curcumin, demethoxycurcumin, bisdemethoxycurcumin is 7:2:1.
3. according to the compositions of protecting gastric mucosa described in claim 1 or 2, it is characterized in that: the particle diameter of described breaking cellular wall Pollen Pini is 3-5 μ m.
4. the compositions of protecting according to claim 3 gastric mucosa, is characterized in that: describedly by the optimum ratio of mass parts, be:
36 parts of breaking cellular wall Pollen Pini
1 part of Rhizoma Curcumae Longae extract.
5. oral administration solid health product of protecting according to claim 1 the compositions of gastric mucosa to prepare; it is characterized in that: can be by pharmaceutically acceptable to the compositions of described protection gastric mucosa and one or more solid excipient or adjuvant being combined by a certain percentage to preparation, described solid excipient or adjuvant are: microcrystalline Cellulose, vegetable oil and/or starch.
6. oral administration solid health product according to claim 5, is characterized in that: the dosage form of described oral administration solid health product is tablet, capsule or pill.
7. protect according to claim 1 the compositions of gastric mucosa in the application of preparing in the medicine of protecting gastric mucosa for one kind.
8. the application of the compositions of protection gastric mucosa according to claim 7 in the medicine of preparation protection gastric mucosa, is characterized in that: the medicine of described protection gastric mucosa is: the medicine for the treatment of gastric ulcer, acute gastritis, chronic gastritis, duodenal ulcer, superficial gastritis and/or chronic atrophic gastritis.
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| CN101444543A (en) * | 2007-11-28 | 2009-06-03 | 仪徐生 | Pine pollen stomach-nourishing tablet |
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