CN1284592C - Chinese materia medica preparation for nourishing face and preparation method - Google Patents
Chinese materia medica preparation for nourishing face and preparation method Download PDFInfo
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- CN1284592C CN1284592C CNB2004100066611A CN200410006661A CN1284592C CN 1284592 C CN1284592 C CN 1284592C CN B2004100066611 A CNB2004100066611 A CN B2004100066611A CN 200410006661 A CN200410006661 A CN 200410006661A CN 1284592 C CN1284592 C CN 1284592C
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Abstract
The present invention relates to a Chinese materia medica preparation for nourishing face and a preparation method thereof. The preparation has health care and treatment functions for symptoms of constipation, menoxenia, etc. caused by stomach intestine dryness-heat and hypofunction of the spleen. The preparation is made from raw materials of aloe, coix seed, tribulus fruit, tree peony bark, dahurian angelica, motherwort, etc. The present invention has the preparation method that aloe, coix seed, tribulus fruit, tree peony bark and dahurian angelica are extracted twice by ethanol, solution extracted by ethanol is merged, refrigerated for storage and filtered, and filtrate is reserved. Except pearl powder, other four kinds of medicine of motherwort, etc. are added with water for decoction for a plurality of times, and decoction liquid is merged, concentrated, precipitated by ethanol, refrigerated for storage and filtered. The filtrate and ethanol extracting solution, such as aloe, etc. are merged, and ethanol is recovered. The merged liquid is depressed, dried and pulverized into fine powder which is mixed with pearl powder. Then, a proper quantity of supplementary materials are added, uniformly mixed, pressed in a dry mode and pelletized. Thus, active components of the medicine of the present invention areobtained.
Description
Technical field
The present invention relates to a kind of skin care Chinese medicine preparation with health care and therapeutical effect, relate in particular to a kind of to gastrointestine dryness heat, deficiency of spleen-QI have the health care and the Chinese medicine preparation of treatment function to symptoms such as constipation, menoxenias, the invention still further relates to this preparation method of Chinese medicine.
Background technology
At present, have multiple skin care class Chinese medicine preparation on the market, the situation of existing like product sees the following form.
Existing skin care class Chinese medicine preparation
| Title | Prescription is formed | Dosage form | Effect | Range of application |
| PAIDU YANGYAN JIAONANG | Radix Et Rhizoma Rhei, Radix Panacis Quinquefolii, Rhizoma Atractylodis Macrocephalae, blue or green Radix Panacis Quinquefolii etc. | Capsule | The catharsis and toxin expelling tonifying spleen and beautifying | Constipation, insufficiency of the spleen |
| The logical beauty nourishing capsule of profit | Aloe, Radix Angelicae Sinensis, Radix Paeoniae, Flos Carthami etc. | Capsule | The catharsis and toxin expelling tonifying spleen and beautifying | Insufficiency of the spleen constipation induced |
| CONGRONG TONGBIAN KOUFUYE | Herba Cistanches, Radix Polygoni Multiflori, vehement real, Mel | Oral liquid | Enriching yin and nourishing kidney, the profit just unimpeded | Constipation |
| The Tongyou dryness moistening ball | Vehement shell, the Radix Aucklandiae, Cortex Magnoliae Officinalis, Semen Persicae, Flos Carthami etc. | Big honeyed pills | Clearing away heat and resolving stagnation of food, the profit just unimpeded | Constipation |
| Constipation is logical | The Rhizoma Atractylodis Macrocephalae, vehement shell, Herba Cistanches etc. | Oral liquid | Invigorating the spleen and benefiting QI, the profit just unimpeded | Constipation |
| Compound Aloe capsule | Aloe, Indigo Naturalis, Cinnabaris, succinum | Capsule | Clearing away heat and moistening the bowels, mind tranquilizing and the heart calming | Habitual constipation |
| Bazhen yimu pills, bazhen yimu wan | Herba Leonuri, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Poria etc. | Honeyed pill | Fill blood, transfer menstruation | Menoxenia |
| The U.S. face treasured of Margarita toxin expelling | Radix Panacis Quinquefolii, Folium Nelumbinis, Radix Rubiae Yunnanensis, blue or green Radix Panacis Quinquefolii etc. | Capsule | Just toxin expelling, tonifying speen and tonifying kidney | Constipation, inappetence |
The problem that existing product exists is to fill a prescription not comprehensively, and fabricating technology falls behind, employing all be traditional Chinese medicinal preparation method, invalid removal of impurity is low.
Summary of the invention
The object of the present invention is to provide a kind of skin care Chinese medicine preparation with health care and therapeutical effect.Said preparation has more specific aim than the compatibility of existing similar prescription, and clinical efficacy is better.
Skin care Chinese medicine preparation of the present invention is mainly made (being parts by weight) by following bulk drugs:
1～4 part of Aloe, 3～12 parts of Cortex Moutans, 4～26 parts of Semen Coiciss, 3～9 parts of the Radixs Angelicae Dahuricae, 4～26 parts of Herba Leonuris, 4～9 parts of Fructus Tribulis, 0.1～0.3 part of Margarita powder, 2～8 parts of Herba Cistanches, 2～8 parts in Herba Spirodelae, 4～15 parts in Fructus Jujubae.
The preparation method of skin care Chinese medicine preparation of the present invention:
(1) 1～4 part of Aloe, 4～26 parts of Semen Coiciss, 4～9 parts of Fructus Tribulis, 3～12 parts of Cortex Moutans, 3～9 parts of the Radixs Angelicae Dahuricae add 60～85% ethanol extractions 2 times of 5～10 times of medical material volumes, and each 1～2 hour, merge alcohol extract, cold preservation filters filtrate for later use;
(2) 4～26 parts of Herba Leonuris, 2～8 parts of Herba Cistanches, 2～8 parts in Herba Spirodelae, 4～15 parts in Fructus Jujubae add 5～10 times of decoctings of medical material volume and boil three times, and each 1～2 hour, merge decoction liquor, concentrate, precipitate with ethanol, cold preservation filters;
(3) alcohol extract in filtrate in (2) and the above-mentioned steps (1) is merged, reclaims ethanol, drying under reduced pressure, be ground into 0.1～0.3 part of mixing of fine powder and Margarita powder after, add appropriate amount of auxiliary materials, mixing, dry-pressing is granulated, packing, promptly.
The active component of medicine of the present invention can be prepared into the medicine or the health product of any peroral dosage form, as capsule, and tablet, granule etc.
The present invention has following advantage:
1. in the Chinese medicine preparation of the present invention, Aloe, Semen Coicis, Fructus Tribuli, Cortex Moutan, the Radix Angelicae Dahuricae, Herba Leonuri, Herba Cistanches, Herba Spirodelae, Fructus Jujubae, Margarita powder be totally ten flavor medical materials, is Chinese medicine tonification medicine commonly used.Aloe purging heat to relax the bowels wherein; The Semen Coicis eliminating damp-heat has the merit of spleen invigorating concurrently; The Fructus Tribuli dispersing the stagnated live-QI to relieve the stagnation of QI; The Cortex Moutan clearing away heat and cooling blood; Radix Angelicae Dahuricae detumescence and apocenosis; The Herba Leonuri inducing diuresis to remove edema; The Herba Cistanches loosening bowel to relieve constipation; Herba Spirodelae, Fructus Jujubae nourishing blood to tranquillize the mind, invigorating the spleen and replenishing QI.Full side's compatibility can be recuperated under medical treatment by internal organs, reaches normal circulation of qi and blood, equilibrium between yin and yang, the effect that function is vigorous.
2. tcm product prescription of the present invention is started with from improving the human internal environment, by absorption and the excretory function of improving body, it is smooth and easy to keep the body blood circulation, remove the health interior-heat, make the metabolism pipeline unblocked, thereby keep the metabolic balance of human body, transfer outside the treatment by oral administration of medicines, reach the effect of permanent beauty treatment.
3. the method for the extraction of Chinese medicine preparation of the present invention and purification has more science, and the dosage form of preparation is advanced modern, taking convenience, and clinical efficacy is good.
Description of drawings
Fig. 1 is preparation technology's flow chart of skin care Chinese medicine preparation of the present invention.
Below by embodiment the present invention is specifically described; be necessary to be pointed out that at this present embodiment only is used for the present invention is further specified; can not be interpreted as limiting the scope of the invention, the person skilled in the art in this field can make some nonessential improvement and adjustment according to the content of the invention described above.
The specific embodiment
Embodiment 1 preparation example
Every day the prescription compatibility of medicines amount
Prescription: Aloe 1.5 grams, Cortex Moutan 10 grams, Semen Coicis 5 grams, the Radix Angelicae Dahuricae 5 grams, Herba Leonuri 5 grams, Fructus Tribuli 5 grams, Margarita powder 0.1 gram, Herba Cistanches 3 grams, Herba Spirodelae 3 grams, Fructus Jujubae 3 grams.
Method for making (in detail referring to Fig. 1): place extraction pot with 5 times 85% soak with ethanol 1 hour Aloe, Semen Coicis, Fructus Tribuli, Cortex Moutan, the Radix Angelicae Dahuricae, reflux, extract, 2 times adds 5 times of amounts of alcohol at every turn, extracted 2 hours, and merged alcohol extract, placed 16 hours, filter filtrate for later use.
Herba Leonuri, Herba Cistanches, Herba Spirodelae, Fructus Jujubaes etc. four are distinguished the flavor of in extraction pot, decoct 3 times, add 10 times of amounts of water at every turn, soaked 1 hour for the first time, the each decoction 1 hour, filter, merge the water extract, being concentrated into relative density is 1.10-1.15 (50 ℃ of surveys), adding ethanol makes and contains alcohol amount and reach 70%, placed 16 hours, and filtered filtrate for later use.
Alcohol extraction filtrates such as water extract-alcohol precipitation filtrate and Aloe are merged, and reclaiming ethanol and being concentrated into relative density is 1.25-1.30 (50 ℃ of surveys).Cool off, add 3 times deionized water, fully stir, left standstill 4 hours.Centrifugal filtration, collecting precipitation, oven dry gets extractum.Extractum is pulverized with multifunctional crusher, crossed 80 mesh sieves.Mix with Margarita powder, add a little adjuvant, make granule.
Embodiment 2 preparation examples
Every day the prescription compatibility of medicines amount
Prescription: Aloe 2 grams, Cortex Moutan 4 grams, Semen Coicis 4 grams, the Radix Angelicae Dahuricae 4 grams, Herba Leonuri 8 grams, Fructus Tribuli 4 grams, Margarita powder 0.3 gram, Herba Cistanches 8 grams, Herba Spirodelae 8 grams, Fructus Jujubae 4 grams.
Method for making: place extraction pot with 10 times 75% soak with ethanol 1 hour Aloe, Semen Coicis, Fructus Tribuli, Cortex Moutan, the Radix Angelicae Dahuricae, reflux, extract, 2 times adds 10 times of amounts of alcohol at every turn, extracts 2 hours, merges alcohol extract, places filtration, filtrate for later use 16 hours.
Herba Leonuri, Herba Cistanches, Herba Spirodelae, Fructus Jujubaes etc. four are distinguished the flavor of in extraction pot, decoct 3 times, add 10 times of amounts of water at every turn, soaked 1 hour for the first time, the each decoction 1 hour, filter, merge the water extract, being concentrated into relative density is 1.10-1.15 (50 ℃ of surveys), adding ethanol makes and contains alcohol amount and reach 60%, placed 16 hours, and filtered filtrate for later use.
Alcohol extraction filtrates such as water extract-alcohol precipitation filtrate and Aloe are merged, and reclaiming ethanol and being concentrated into relative density is 1.25-1.30 (50 ℃ of surveys).Cool off, add 3 times deionized water, fully stir, left standstill 4 hours.Centrifugal filtration, collecting precipitation, oven dry gets extractum.Extractum is pulverized with multifunctional crusher, crossed 80 mesh sieves.Mix with Margarita powder, add adjuvant, make capsule.
Embodiment 3 preparation examples
Every day the prescription compatibility of medicines amount
Aloe 3 grams, Cortex Moutan 6 grams, Semen Coicis 3 grams, the Radix Angelicae Dahuricae 9 grams, Herba Leonuri 20 grams, Fructus Tribuli 6 grams, Margarita powder 0.3 gram, Herba Cistanches 2 grams, Herba Spirodelae 2 grams, Fructus Jujubae 6 grams.
Method for making: place extraction pot with 10 times 60% soak with ethanol 1 hour Aloe, Semen Coicis, Fructus Tribuli, Cortex Moutan, the Radix Angelicae Dahuricae, reflux, extract, 2 times adds 10 times of amounts of alcohol at every turn, extracts 2 hours, merges alcohol extract, places filtration, filtrate for later use 16 hours.
Herba Leonuri, Herba Cistanches, Herba Spirodelae, Fructus Jujubaes etc. four are distinguished the flavor of in extraction pot, decoct 3 times, add 5 times of amounts of water at every turn, soaked 1 hour for the first time, the each decoction 1 hour, filter, merge the water extract, being concentrated into relative density is 1.10-1.15 (50 ℃ of surveys), adding ethanol makes and contains alcohol amount and reach 70%, placed 16 hours, and filtered filtrate for later use.
Alcohol extraction filtrates such as water extract-alcohol precipitation filtrate and Aloe are merged, and reclaiming ethanol and being concentrated into relative density is 1.25-1.30 (50 ℃ of surveys).Cool off, add 3 times deionized water, fully stir, left standstill 4 hours.Centrifugal filtration, collecting precipitation, oven dry gets extractum.Extractum is pulverized with multifunctional crusher, crossed 80 mesh sieves.Mix with Margarita powder, add a little adjuvant, compacting in flakes.
Embodiment 4 pilot scales
Get the medical material of 500 times of embodiment recipe quantities 1 every day:
Aloe, Semen Coicis, Fructus Tribuli, Cortex Moutan, Radix Angelicae Dahuricae alcohol extraction 2 times merge alcohol extract, and cold preservation filters filtrate for later use.Except that Margarita powder, all the other 4 flavor gradation such as Herba Leonuri decoct with water, and merge decoction liquor, concentrate, and precipitate with ethanol, cold preservation filters.Alcohol extract such as filtrate and above-mentioned Aloe merges, and reclaims ethanol, and drying under reduced pressure after being ground into fine powder and Margarita powder and mixing, adds appropriate amount of auxiliary materials, mixing, and dry-pressing is granulated, and packing is made 1000 bag granules, promptly.
Produce three batches according to above preparation technology, the result is as follows:
| Lot number | Inventory (kg) | Extractum amount (kg) | Barbaloin content (mg/ bag) |
| 20021025 | 190 | 20.5 | 58.5 |
| 20021026 | 190 | 19.8 | 57.4 |
| 20021027 | 190 | 19.9 | 58.2 |
Carry out pilot scale according to above preparation technology, the extractum yield is about 10%, and barbaloin content is not less than the 50mg/ bag, and it is stable to make end product quality, and each dose is 1 bag granule, and wrap every days 2.
The pharmacological research experimental example of embodiment 5-8 skin care Chinese medicine preparation of the present invention
Be subjected to the reagent thing:
The drug extract powder of the embodiment of the invention 4: every gram extract powder is equivalent to 12.20 gram crude drugs.Be made into debita spissitudo before the test, for the animal gastric infusion.
PAIDU YANGYAN JIAONANG: Yunnan Panlongyunhai Pharmaceutical Co., Ltd. produces, lot number: 010215.Become debita spissitudo with preceding with distilled water diluting, for the animal gastric infusion.
Experimental animal:
Km kind mice, Wistar rat are raised in the observation ward of central air-conditioning is arranged, the full nutrition piece material that feedstuff is produced for Nat'l Pharmaceutical ﹠ Biological Products Control Institute's animal center, drink tap water.
The laxation defecating feces excretion of embodiment 5 Chinese medicine preparation of the present invention
A. to rat gastric secretion and the excretory influence of gastric acid
Select 50 rats for use, male and female each half, body weight 250-300g.Be divided into 5 groups at random, 10 every group.(1) normal control group is irritated stomach and is given distilled water; (2) positive controls is irritated stomach and is given the PAIDU YANGYAN JIAONANG suspension; (3) drug extract powder 16g crude drug of the present invention/kg dosage group; (4) drug extract powder 8g crude drug of the present invention/kg dosage group; (5) drug extract powder 4g crude drug of the present invention/kg dosage group, three groups of backs are irritated stomach and are given an amount of drug extract powder medicinal liquid of the present invention.Perfusion every day once, successive administration 15 days, in last administration fasting in preceding 24 hours, after the administration 30 minutes, with etherization, open the abdominal cavity, the ligation pylorus is sewed up abdominal part then, water is prohibited in fasting.After 2 hours, with the pentobarbital sodium anesthetized animal, open the abdominal cavity, the ligation cardia takes out stomach, pours gastric juice into centrifuge tube, and is centrifugal, takes out supernatant to graduated cylinder, measures gastric juice amount and record.With pipette, extract 2ml gastric juice, to little triangular flask, drip phenolphthalein indicator.With 0.01N NaOH titration, measure total acidity gastric juice and total acid content, result's (seeing Table 1) shows, after drug extract powder filling stomach of the present invention gives rat, can obviously promote gastric secretion, improves gastric acid content, each group of administration more all has significant difference with matched group.
Table 1. medicine of the present invention is to rat gastric secretion and the excretory n=10 that influences of gastric acid
| Grouping | Dosage (the g crude drug/kg) | Gastric juice volume (m1) | Gastric acidity (mmol/L) | Total acidity gastric juice (mmol/L.h) |
| Matched group | 0 | 9.27±2.49 | 24.9±5.5 | 76.0±24.6 |
| PAIDU YANGYAN JIAONANG | 4.8g powder/kg | 6.3±1.9 | 34.5±13.1 * | 70.3±33.3 |
| Medicine of the present invention | 16 | 11.69±3.69 | 51.1±9.2 *** | 193±48.0 *** |
| Medicine of the present invention | 8 | 7.73±3.57 | 47.5±12.1 *** | 127.6±63.2 * |
| Medicine of the present invention | 4 | 11.30±3.19 | 50.4±13.5 | 188.2±46.5 |
*P＜0.05
**P＜0.01
***P＜0.001
B. to the influence of rat stomach proteinase activity and to the protective effect of gastric mucosa
Test is divided into groups, test method is the same.With the centrifugal gastric juice supernatant of pipette, extract 2ml, insert in the 10ml digestion bottle, put into ready made long 1.5cm, the protein pipe of diameter 0.5cm, digestion is 24 hours in 37 ℃ of calorstats, after the taking-up, measure the length of two sections transparent parts of protein pipe with precimeter, as weighing pepsin activity value index, the administration cell mean is made comparisons, carry out statistics T test, result's (seeing Table 2) shows 16g crude drug/kg, 8g crude drug/kg promptly two groups with matched group significant difference is arranged more all, prove that medicine of the present invention can significantly improve pepsic activity, promote proteopepsis.The body of stomach that will take out gastric juice in addition flattens, and is attached on the filter paper, puts into the Specimen bottle that fills formalin, and after fixing, the ulcer that the meat flame detects on the gastric body mucosa is counted out; And measure each ulcer spot diameter with precimeter, by following standard marking: diameter＞2mm is 3 minutes; Diameter 1-2mm is 2 minutes; Be 1 minute below the diameter 1mm; No ulcer point is 0 minute; administration group marking meansigma methods is compared with matched group; carry out statistics T test; result's (seeing Table 8-2) shows; the degree that ulcer takes place is respectively organized in administration, all is lighter than matched group, and particularly 8g crude drug/kg dosage group and matched group are relatively; evident difference is arranged, show that this medicine has the certain protection effect to gastric mucosa.
Table 2. medicine of the present invention is to the influence of rat stomach proteinase activity and the protective effect of gastric mucosa
| Grouping | Dosage (the g crude drug/kg) | Pepsin activity (mm) | Gastric mucosa ulcer level (score value) |
| Matched group | 0 | 2.34±2.89 | 4.7±3.7 |
| PAIDU YANGYAN JIAONANG | 4.8g powder/kg | 2.09±1.46 | 6.1±6.2 |
| Medicine of the present invention | 16 | 5.96±1.84 *** | 2.3±2.5 |
| Medicine of the present invention | 8 | 6.65±7.18 * | 1.4±1.84 * |
| Medicine of the present invention | 4 | 3.66±2.57 | 3.2±3.5 |
*P＜0.05
**P＜0.001
C. to the propulsive influence of mice intestinal
50 of Km kind mices, male and female half and half, body weight 22-25g are selected in test for use.Be divided into 5 groups at random, 10 every group, (1) matched group is irritated stomach and is given with the volume distilled water; (2) positive drug control group is irritated stomach and is given PAIDU YANGYAN JIAONANG mixing suspension; (3) medicine high dose group of the present invention is irritated stomach and is given 16g crude drug/kg dosage medicinal liquid; (4) dosage group in the medicine of the present invention is irritated stomach and is given 8g crude drug/kg dosage medicinal liquid; (5) medicine low dose group of the present invention is irritated stomach and is given 4g crude drug/kg dosage medicinal liquid.Gavage equal volume every day one time, the medicine of variable concentrations gives 15 days continuously.After last administration 1 hour, every treated animal gavages 0.5ml/ burnt black ink (commercially available Ostriches board, Tianjin Ink Factory produces), after 20 minutes, take off neck and put to death animal, fry in shallow oil the portion of cutting open the belly, take out the stomach cardia, separate, even up to the anus intestinal segment, measure total length and ink and advance length, to advance average rate is index, and administration group and matched group relatively carry out statistics T test, result's (seeing Table 3) shows, medicine of the present invention has certain promotion to the mice propulsion functions, and particularly 4g crude drug/kg dosage group relatively has significant difference with matched group.
Table 3. medicine of the present invention is to the influence of mice intestinal propulsion functions
| Grouping | Dosage (the g crude drug/kg) | Advance percentage rate % |
| Matched group | 0 | 63.6±18.2 |
| PAIDU YANGYAN JIAONANG | 2.2g powder/kg | 70.1±10.4 |
| Medicine of the present invention | 16 | 66.5±13.0 |
| Medicine of the present invention | 8 | 73.7±12.5 |
| Medicine of the present invention | 4 | 82.3±7.7 ** |
**P＜0.01
D. to the influence of normal mouse defecation
50 of Km kind mices, male and female half and half, body weight 22-25g are selected in test for use.Be divided into 5 groups at random, 10 every group, (1) matched group is irritated stomach and is given with the volume distilled water; (2) positive drug control group is irritated stomach and is given PAIDU YANGYAN JIAONANG mixing suspension; (3) medicine high dose group of the present invention is irritated stomach and is given 16g crude drug/kg dosage medicinal liquid; (4) dosage group in the medicine of the present invention is irritated stomach and is given 8g crude drug/kg dosage medicinal liquid; (5) medicine low dose group of the present invention is irritated stomach and is given 4g crude drug/kg dosage medicinal liquid.Gavage equal volume every day one time, the medicine of variable concentrations gives 15 days continuously.In last administration fasting in preceding 20 hours, after the last administration 1 hour, every treated animal gavages 0.5ml/ burnt black ink (commercially available Ostriches board, Tianjin Ink Factory produces), then mice is placed in the white mice rearging cage of no bedding and padding, the observed and recorded mice is row's melena grain number in row's melena time and 6 hours, administration group and matched group are carried out statistics T test, result's (seeing Table 4) as seen, medicine of the present invention can highly significant shortening mice row's melena time, increase defecation grain number in the certain hour, defecation, relieving constipation are had good effect.
Table 4. medicine of the present invention is to the influence of mice defecation
| Grouping | Dosage (the g crude drug/kg) | Row's melena time (branch) | Row's melena number (grain) in 6 hours | The feces character |
| Matched group | 0 | 235.2±68.8 | 5.4±3.4 | Granular |
| PAIDU YANGYAN JIAONANG | 2.2g powder/kg | 175.4±29.1 * | 13.8±2.1 *** | Loose stool, shapeless |
| Medicine of the present invention | 16 | 127.1±37.1 *** | 12.2±3.3 *** | Soft stool is shaped |
| Medicine of the present invention | 8 | 175.1±43.9 * | 9.6±3.9 * | The part soft stool |
| Medicine of the present invention | 4 | 173.7±49.3 * | 6.3±2.4 | Indivedual soft stools |
*P＜0.05
***P＜0.001
The regulating menstruation and activating blood effect of embodiment 6 Chinese medicine preparation of the present invention
A. to the influence of rat serum viscosity
Select 40 rats for use, male and female dual-purpose, body weight 250-300g.Be divided into 5 groups at random.(1) normal control group (9) is irritated stomach and is given distilled water; (2) positive drug control group (8) is irritated stomach and is given PAIDU YANGYAN JIAONANG mixing suspension; (3) medicine 16g crude drug of the present invention/kg dosage group (7); (4) medicine 8g crude drug of the present invention/kg dosage (8); (5) medicine 4g crude drug of the present invention/kg dosage (8).Three groups of backs are irritated stomach and are given an amount of medicine medicinal liquid of the present invention.Irritate stomach every day once, successive administration 15 days, after the last administration 1 hour, the pentobarbital sodium anesthetized animal was won eyeball, and blood is flowed in the centrifuge tube that contains heparin sodium naturally, shakes while flowing and makes the blood anticoagulant.Shake up after leaving standstill, measure whole blood viscosity and packed cell volume (HCT).Result's (seeing Table 5) shows: medicine gastric infusion of the present invention, can low whole blood viscosity, the particularly 16g crude drug/kg dosage group that reduces of highly significant compare statistical significance with matched group.
Table 5. medicine of the present invention is to the influence of rat serum viscosity
| Grouping | Dosage (the g crude drug/kg) | Number of animals (only) | HCT(％) | High shear rate | Low shear rate |
| Matched group | 0 | 9 | 49.11±1.69 | 5.31±0.19 | 10.55±0.21 |
| PAIDU YANGYAN JIAONANG | 4.8 | 8 | 49.75±2.49 | 4.95±0.45- | 9.76±1.01 * |
| Medicine of the present invention | 16 | 7 | 46.57±2.64 * | 4.80±0.31 ** | 9.22±0.78 *** |
| Medicine of the present invention | 8 | 8 | 48.75±2.6 | 4.96±0.29 | 9.79±0.79 |
| Medicine of the present invention | 4 | 8 | 46.63±3.38 | 4.67±0.31 ** | 9.49±0.83 ** |
*P＜0.05
*P＜0.01
***P＜0.001
B. to the influence of Mouse Uterus, ovary coefficient
50 of Km kind mices are selected in test for use, and male and female are about body weight 15g.Be divided into 5 groups at random, 10 every group, (1) matched group is irritated stomach and is given with the volume distilled water; (2) positive drug control group is irritated stomach and is given PAIDU YANGYAN JIAONANG mixing suspension; (3) medicine high dose group of the present invention is irritated stomach and is given 16g crude drug/kg dosage medicinal liquid; (4) dosage group in the medicine of the present invention is irritated stomach and is given 8g crude drug/kg dosage medicinal liquid; (5) medicine low dose group of the present invention is irritated stomach and is given 4g crude drug/kg dosage medicinal liquid.Gavage equal volume every day one time, the medicine of variable concentrations gives 15 days continuously.Last administration fasting in preceding 16 hours, after the administration 1 hour, take off neck and put to death mice, weigh, dissect animal, taking out uterus and ovary, weigh after peeling off fatty tissue, is index with the two weight and weight ratio, administration group and matched group are tested as statistics T, result's (seeing Table 6) shows: medicine of the present invention has weightening finish trend to the Mouse Uterus coefficient, but there was no significant difference does not then have influence to the ovary coefficient.
Table 6 medicine of the present invention is to the influence of Mouse Uterus, ovary coefficient
| Grouping | Dosage (the g crude drug/kg) | Weight average value (g) | Uterus coefficient (mg/g) | Ovary coefficient (mg/g) |
| Matched group | 0 | 23.1±1.4 | 1.004±0.248 | 0.384±0.046 |
| PAIDU YANGYAN JIAONANG | 2.2g/kg | 22.6±3.0 | 1.327±1.205 | 0.447±0.131 |
| Medicine of the present invention | 16 | 23.2±2.5 | 1.337±1.023 | 0.392±0.151 |
| Medicine of the present invention | 8 | 24.2±2.3 | 1.101±0.761 | 0.192±0.041 |
| Medicine of the present invention | 4 | 22.9±2.7 | 1.117±0.318 | 1.286±0.064 |
The eliminating blood stasis to promote regeneration of blood effect of embodiment 7 medicines of the present invention
A. to the influence of rat blood serum protein content
Select 40 rats for use, male and female dual-purpose, body weight 250-300g.Be divided into 5 groups at random.(1) normal control group (9) is irritated stomach and is given distilled water; (2) positive drug control group (8) is irritated stomach and is given PAIDU YANGYAN JIAONANG mixing suspension; (3) medicine 16g crude drug of the present invention/kg dosage group (7); (4) medicine 8g crude drug of the present invention/kg dosage (8); (5) medicine 4g crude drug of the present invention/kg dosage (8).Three groups of backs are irritated stomach and are given an amount of medicine medicinal liquid of the present invention.Irritate stomach every day once, successive administration 15 days, after the last administration 1 hour, the pentobarbital sodium anesthetized animal is won eyeball, and blood is flowed in the centrifuge tube that contains heparin sodium naturally, after leaving standstill, 3000 leave the heart, survey serum albumin, total protein content with full automatic biochemical apparatus, and result's (seeing Table 7): medicine of the present invention does not have influence to rat blood serum albumin, total protein content.
Table 7 medicine of the present invention is to the influence of rat blood serum albumin, total protein content
| Grouping | Dosage (the g crude drug/kg) | Number of animals (only) | Albumin (mg/ml) | Total protein (mg/ml) |
| Matched group | 0 | 9 | 39.95±1.42 | 60.56±2.68 |
| PAIDU YANGYAN JIAONANG | 4.8g powder/kg | 8 | 33.74±1.43 | 58.56±3.45 |
| Medicine of the present invention | 16 | 7 | 33.72±1.98 | 61.44±4.42 |
| Medicine of the present invention | 8 | 8 | 33.39±3.26 | 57.86±4.74 |
| Medicine of the present invention | 4 | 8 | 33.00±2.83 | 57.34±3.64 |
B. to liver tissues of rats SOD activity, fat brown fat content; The active influence of the autonomous MAO of brain
Select 41 rats for use, male and female dual-purpose, body weight 250-300g.Be divided into 5 groups at random.(1) normal control group (9) is irritated stomach and is given distilled water; (2) positive drug control group (8) is irritated stomach and is given PAIDU YANGYAN JIAONANG mixing suspension; (3) medicine 16g crude drug of the present invention/kg dosage group (8); (4) medicine 8g crude drug of the present invention/kg dosage (8); (5) medicine 4g crude drug of the present invention/kg dosage (8).Three groups of backs are irritated stomach and are given an amount of medicine medicinal liquid of the present invention.Irritate stomach every day once, successive administration 15 days, after the last administration 1 hour, the pentobarbital sodium anesthetized animal is opened abdominal part, takes out liver, clean with normal saline flushing, accurately take by weighing hepatic tissue 0.2g, add 9 times of normal saline, be prepared into 10% liver tissue homogenate, 3500 rev/mins, centrifugal 10 minutes, get supernatant 0.1ml, add the 0.9ml normal saline and be diluted to 1% liver tissue homogenate, measure the medicine box operating provision by superoxide dismutase (SOD) and operate, measure the SOD activity, the results are shown in Table 8.Other gets 1% liver tissue homogenate 0.5, add the 0.5ml normal saline, be diluted to 0.5% liver tissue homogenate, use the spectrophotometry protein content, the results are shown in Table 8, get 10% brain tissue homogenate, 3500 rev/mins, centrifugal 10 minutes, press monoamine oxidase, MAO (MAO) and measure the medicine box operating procedure, measure, the results are shown in Table 9.Get the 10% supernatant 0.2ml of brain tissue homogenate, add the 0.6ml normal saline, be diluted to 2.5% concentration,, the results are shown in Table 9 with protein content in the spectrophotometry cerebral tissue.More than each result show that medicine of the present invention has certain influence to rat liver protein content and SOD activity, 4g crude drug/kg dosage can make protein content reduce, SOD is active to be increased; And 16g crude drug/kg dosage does not have obvious influence to protein content, but can make the active reduction of SOD, and this medicine of results suggest becomes two-way function to removing free radical.But to protein content, the obviously influence of the active nothing of MAO in liver fat brown fat content and the cerebral tissue.
Table 8. medicine of the present invention is to rat liver SOD enzyme, fat brown fat, proteic influence
| Grouping | Dosage (the g crude drug/kg) | Number of animals (only) | Protein content (mgrot/ml) | SOD activity (Nu/mgprot) | Fat brown fat content (μ g/g hepatic tissue) |
| Matched group | 0 | 9 | 2.127±0.321 | 110.70±4.58 | 0.785±0.023 |
| PAIDU YANGYAN JIAONANG | 4.8g powder/kg | 8 | 1.885±0.050 * | 86.14±13.11 ** | 0.940±0.048 * |
| Medicine of the present invention | 16 | 8 | 1.940±0.096 | 88.38±9.19 ** | 0.807±0.0473 |
| Medicine of the present invention | 8 | 8 | 1.983±0.174 | 100.24±12.82 | 0.777±0.045 |
| Medicine of the present invention | 4 | 8 | 1.79±0.144 * | 132.31±7.37 ** | 0.853±0.046 |
*P＜0.05
**P＜0.01
Table 9 medicine of the present invention is to rat cerebral tissue's protein content, the active influence of MAO
| Grouping | Dosage (the g crude drug/kg) | Number of animals (only) | Protein content (mgprot/ml) | MAO activity (U/h/mgprot) |
| Matched group | 0 | 9 | 3.756±0.266 | 6.934±1.267 |
| PAIDU YANGYAN JIAONANG | 4.8g powder/kg | 8 | 3.610±0.287 | 7.324±0.741 |
| Medicine of the present invention | 16 | 8 | 3.728±0.224 | 6.503±0.741 |
| Medicine of the present invention | 8 | 8 | 3.870±0.296 | 7.295±1.313 |
| Medicine of the present invention | 4 | 8 | 3.92±0.109 | 6.326±0.845 |
The clearing away heat-damp and promoting diuresis effect of embodiment 8 Chinese medicine preparation of the present invention
A. to the bullate influence of rat carrageenan foot
Select 50 rats for use, male and female, body weight about 250.Be divided into 5 groups at random.(1) normal control group is irritated stomach and is given distilled water; (2) positive drug control group is irritated stomach and is given PAIDU YANGYAN JIAONANG mixing suspension; (3) medicine 16g crude drug of the present invention/kg dosage group; (4) medicine 8g crude drug of the present invention/kg dosage; (5) medicine 4g crude drug of the present invention/kg dosage.Three groups of backs are irritated stomach and are given an amount of medicine medicinal liquid of the present invention.Irritate stomach every day once, successive administration 7 days, after the last administration, the right back more sufficient pad subcutaneous injection 1% carrageenin suspension 0.05ml of portion induces the generation of inflammation.Respectively cause scorching before and cause scorching back 0.5,1,2,3,4 hour, survey with projector (amplifying 6.5 times) and cause 0.5cm place diameter under the scorching limb ankle joint, consequently the difference of inflammation front and back is as the swelling degree of inflammation.Average and the matched group of getting each administration group compare, and carry out statistics T test.Result's (seeing Table 10) shows that medicine of the present invention is to the swollen antiinflammatory action that highly significant is arranged of rat carrageenan foot.
Table 10. medicine of the present invention is to the bullate influence of rat carrageenan foot
| Grouping | Dosage (the g crude drug/kg) | Normal value (cm) | The swollen degree of foot (Δ X ± SD) | ||||
| 0.5 hour | 1 hour | 2 hours | 3 hours | 6 hours | |||
| Matched group | 0 | 5.24±0.18 | 0.29± 0.19 | 0.59± 0.35 | 0.80± 0.49 | 1.15±0.42 | 0.49± 0.57 |
| PAIDU YANGYAN JIAONANG | 4.8g/kg | 5.29±0.14 | 0.10± 0.11 * | 0.25± 0.10 * | 0.42± 0.19 * | 0.61± 0.22 ** | 0.56± 0.19 |
| Medicine of the present invention | 16 | 5.28±0.16 | 0.13± 0.16 | 0.21± 0.13 ** | 0.40± 0.23 * | 0.64± 0.24 ** | 0.60± 0.21 |
| Medicine of the present invention | 8 | 5.27±0.18 | 0.24± 0.31 | 0.36± 0.27 | 0.42± 0.28 * | 0.66± 0.39 * | 0.76± 0.44 |
| Medicine of the present invention | 4 | 5.35±0.26 | 0.18± 0.11 | 0.34± 0.15 | 0.46± 0.23 | 0.71± 0.26 * | 0.86± 0.29 |
*P＜0.05
**P＜0.01
Claims (3)
1. skin care Chinese medicine, the effective ingredient that it is characterized in that this medicine is to be made by following bulk drugs:
1～4 part of Aloe, 3～12 parts of Cortex Moutans, 4～26 parts of Semen Coiciss, 3～9 parts of the Radixs Angelicae Dahuricae, 4～26 parts of Herba Leonuris, 4～9 parts of Fructus Tribulis, 0.1～0.3 part of Margarita powder, 2～8 parts of Herba Cistanches, 2～8 parts in Herba Spirodelae, 4～15 parts in Fructus Jujubae.
2. the described skin care Chinese medicine of claim 1 is characterized in that this medicine is a peroral dosage form.
3. the described skin care preparation method of Chinese medicine of claim 1 is characterized in that:
(1) Aloe 1～4 weight portion, 4～26 parts of Semen Coiciss, 4～9 parts of Fructus Tribulis, 3～12 parts of Cortex Moutans, 3～9 parts of the Radixs Angelicae Dahuricae add 60～85% ethanol extractions 2 times of 5～10 times of medical material volumes, and each 1～2 hour, merge alcohol extract, cold preservation filters filtrate for later use;
(2) 4～26 parts of Herba Leonuris, 2～8 parts of Herba Cistanches, 2～8 parts in Herba Spirodelae, 4～15 parts in Fructus Jujubae, the decocting that adds 5～10 times of medical material volumes boils three times, and each 1～2 hour, merge decoction liquor, concentrate, precipitate with ethanol, cold preservation filters;
(3) alcohol extract in filtrate in (2) and the above-mentioned steps (1) is merged, reclaiming ethanol and being concentrated into relative density is 1.25-1.30, and cooling adds deionized water, fully stirs, and leaves standstill; Centrifugal filtration, collecting precipitation, oven dry gets extractum; Pulverize extractum, after 0.1～0.3 part of Margarita powder mixes, add appropriate amount of auxiliary materials, mixing, dry-pressing is granulated, packing, promptly.
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