CN1908172A - Broom corn millet drought-resistant regulation gene MYB transcription factor gene and application thereof - Google Patents
Broom corn millet drought-resistant regulation gene MYB transcription factor gene and application thereof Download PDFInfo
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Abstract
The invention discloses a broom corn millet drought-resistant regulation gene MYB transcription factor gene and application thereof, wherein the genome sequence of the gene is 1739bp, and the gene comprises two introns of 121bp (347-467 bp) and 93bp (599-691 bp) and three exons; the full-length cDNA sequence is 1525bp, wherein the 3 'untranslated region is 212bp, the 5' untranslated region is 41bp, the coding region is 1272bp, 424 amino acids are coded, and a serine (Ser, S) rich region exists at the C-terminal. RT-PCR expression analysis of the gene shows that the gene has a certain amount of expression under normal conditions, is induced to express under drought stress (the relative water content of soil is 36 percent), is inhibited under severe stress (the relative water content of soil is 24 percent), has the highest expression level 2 hours after rehydration, and then is reduced to a level lower than the normal growth level. The gene is used for transforming crops such as wheat, corn and the like and other plants, the drought resistance and the water utilization efficiency of the crops are improved, and the gene has great application potential.
Description
Technical field
The invention belongs to the plant gene engineering technology field.Be particularly related to a drought-resisting regulating gene MYB transcription factor gene that derives from broom corn millet, its proteins encoded and efficiently utilize purposes in the plant cultivating drought resisting and moisture.
Background technology
Crop drought resistance is main by separate the corresponding gene of reacting and regulating proteins associated matter synthetic enzyme with biological lack of moisture from multiple biology (comprising microorganism, plant and animal) with the genetic improvement research of water use efficiency at present.The gene such as transcription factor that successfully will clone proline synthase gene, trehalose synthesize enzyme gene, transmission signal and regulate gene expression from microorganism, insect and plant and different promotors are in conjunction with importing plant (as beet, tobacco, plan nanmu leaf mustard etc.), obtain effective expression, the drought tolerance of " engineered plant " and winter hardiness have certain enhancing, showed very tempting prospect, still furtherd investigate at present.
Broom corn millet (Panicum miliaceum L.) belongs to Gramineae Panicum (Panicum), is a kind of ancient gramineous crop that originates from China, brings into play important effect in the development of Chinese nation.Have characteristics such as drought-enduring, degeneration-resistant, water use efficiency height, it is one of crop of drought resisting in the cereal crop, be the excellent genetic resources of plant drought resistance improvement, by retrieval, do not find any open or report broom corn millet drought-resisting and the efficient pertinent literature that utilizes relevant regulatory gene clone of moisture.
Summary of the invention
The objective of the invention is to, broom corn millet drought-resisting regulating gene MYB transcription factor gene and uses thereof is provided.The present invention adopts and suppresses the cDNA library that the subtractive hybridization technology has made up broom corn millet drought-resisting germplasm rehydration and arid, rehydration and normal irrigation respectively, carried out a large amount of random sequencings to obtaining the clone in the library, therefrom screening has obtained the partial sequence of broom corn millet drought-resisting regulating gene myb transcription factor gene, and then utilize 3 ' RACE and electronic cloning technology to combine, obtain the genomic dna sequence and the cDNA sequence of this gene.The genome sequence of the broom corn millet MYB class transcription factor gene that obtains is classified 1739bp (seeing accompanying drawing 1) as, and this gene comprises two introns, is respectively 121bp (347-467bp) and 93bp (599-691bp), three exons.The full-length cDNA of the broom corn millet MYB class transcription factor gene PmMYB that obtains is 1525bp (seeing accompanying drawing 2), and wherein 3 ' non-translational region is 212bp, and 5 ' non-translational region is 41bp, and the coding region is 1272bp, 424 amino acid of encoding altogether.There is a Serine in its C-end, and (Ser, S) abundant district has transcriptional activity.
The RT-PCR expression analysis of broom corn millet drought-resisting regulating gene MYB transcription factor gene of the present invention shows, this gene has a certain amount of expression under normal circumstances, (soil relative water content is 36%) abduction delivering under drought stress, severe is coerced down (soil relative water content 24%) expression and is suppressed, the 2h expression amount is the highest after the rehydration, drops to the level also lower than normal growth subsequently.Illustrate that this gene mainly participates in drought stress initial stage signal conductive process, the functional gene of the relevant arid defence of regulation and control.This gene is broom corn millet drought-resisting and moisture key for high-efficient use regulatory gene, utilizes plants such as its transformed wheat, corn, starts corresponding downstream gene, improves its drought resistance and water use efficiency, has very big application potential.
Description of drawings
Fig. 1 is the genome sequence of broom corn millet MYB class transcription factor gene;
Fig. 2 is the full length cDNA sequence of broom corn millet MYB class transcription factor gene;
Fig. 3 is broom corn millet MYB class transcription factor gene full length cDNA sequence and deduced amino acid, and wherein: dash area is that R2, R3 repeat;
Be the transcriptional activation domain of inferring;
Fig. 4 is the hydrophobicity analysis of broom corn millet MYB class transcription factor gene encoding amino acid sequence;
Fig. 5 is the conservative domain analysis of the structure of broom corn millet MYB class transcription factor gene encoding amino acid sequence;
Fig. 6 is the multiple comparisons of several plant MYB class transcription factor gene aminoacid sequence R2R3 tumor-necrosis factor glycoproteins;
Fig. 7 is based on the systematic evolution tree of the MYB class transcription factor gene of aminoacid sequence structure;
Fig. 8 is that the RT-PCR that broom corn millet MYB class transcription factor gene is expressed under different in moisture processing and rehydration condition analyzes.
The present invention is described in further detail below in conjunction with embodiment that accompanying drawing and contriver provide.
Embodiment
1. the clone of broom corn millet drought-resisting regulating gene MYB transcription factor gene
MYB class transcription factor family is one of family maximum in the plant transcription factor, plays important effect in the transcriptional control of plant.The common trait of MYB class transcription factor gene be contain multiple MYB structural domain that 1-3 is made up of 51-53 amino acid, incomplete (R1, R2, R3).Generally contain 3 conservative tryptophanes (W) residue in each MYB structural domain, often (I, L F) replace first tryptophane of R3MYB structural domain by hydrophobic amino acid in plant R2R3-MYB albumen.
The present invention has made up broom corn millet rehydration and arid respectively by suppressing subtractive hybridization (SSH) technology, the cDNA library of rehydration and normal irrigation, carried out random sequencing to obtaining the clone in the library, therefrom screening has obtained the partial sequence (RS366 of broom corn millet drought-resisting regulating gene MYB transcription factor gene, the GenBank number of including DW177353), has higher homology with paddy rice MYB class transcription factor gene (MYB18), nucleotide sequence according to paddy rice MYB18, designed two pairs of primers (eRS366-1: forward 5-AGTTGCTGCCGTTCTCCCA, oppositely 5-CTGGTGACCTATTGAGCCCTGT; ERS366-2: forward 5-TTTCCACTGTGATGGCA, reverse 5-ATCTGGTGCTCTGTTCC), genomic dna to the broom corn millet drought-resisting germplasm increases, first pair of primer amplification goes out the fragment of a 1267bp, second pair of primer amplification goes out the fragment of 541bp, two sequences is through splicing, the genome sequence that obtains the broom corn millet myb gene is classified 1739bp (sequence is seen accompanying drawing 1) as, its structural analysis is shown, this gene includes two introns, be respectively 121bp (347-467bp), 93bp (599-619bp), three exons.
Comprise in the 20 μ L reaction systems: the cDNA first chain template 1 μ L, 10 * PCR Buffer, 2 μ L, dNTP (2mmol/L) 2 μ L, forward primer (10mmol/L) 1 μ L, reverse primer (10mmol/L) 1 μ L, Taq DNA polymerase (5U/ μ L) 0.2 μ L, ddH
2O polishing to 20 μ L.
The PCR response procedures is: 95 ℃ of pre-sex change 3min, then carry out 95 ℃ of 40s, 52-55 ℃ of (55 ℃ of the first pair of primers, second couple primer 52 ℃) 40s, 72 (90s, totally 35 circulations; In 72 ℃ of extension 8min, the reaction back is in 4 ℃ of insulations at last.1% agarose gel electrophoresis detects amplified production.
Utilize primer (5-AGTTGCTGCCGTTCTCCCA-3 and 5-ATCTGGTGCTCTGTTCC-3), adopt the RT-PCR method after the broom corn millet arid, to increase the cDNA under the rehydration condition and obtained the full length cDNA sequence (seeing accompanying drawing 2) of broom corn millet drought-resisting regulating gene MYB transcription factor gene, full-length cDNA is 1525bp, wherein 3 ' non-translational region is 212bp, 5 ' non-translational region is 41bp, the coding region is 1272bp, 424 amino acid (seeing accompanying drawing 3) of encoding altogether.
2. the initial analysis of broom corn millet drought-resisting regulating gene MYB transcription factor gene encoding amino acid sequence
Most of myb transcription factors have transcriptional activation function, and the transcriptional activation function territory generally is present in proteic centre or C-end, and often to be rich in acidic amino acid, proline(Pro), glutamine or serine/threonine are feature.Analyze broom corn millet drought-resisting regulating gene MYB transcription factor gene, (Ser, S) abundant district infers that this myb gene has transcriptional activity to find there is a Serine at its C-end.
Software prediction, this albumen PI is 6.71, MW is 47273.90, has a little water repellent region (the 70-90 amino acids is seen accompanying drawing 4).This albumen is positioned in the nucleus, and this function with its participation genetic transcription is consistent.
The conserved structure domain analysis of this gene coding amino acid shows (seeing accompanying drawing 5), this gene has the DNA land (DNA-binding domain) of two typical MYB class transcription factor genes, be respectively 13-63,66-114 amino acids, this gene belongs to typical R 2R3-MYB transcription factor.(Trp, W) (Phe F) replaces first tryptophan residue of its R3 structural domain by phenylalanine.
To deriving from paddy rice (Oryza sativa), broom corn millet (Panicum miliaceum), corn (Zeamays), torch pine (Pinus taeda), Arabidopis thaliana (Arabidopsis thaliana), capsicum (Capsicum annuum), upland cotton (Gossypium hirsutum), the R2 of the myb gene of barley (Hordeumvulgare) and eggplant nine kind of plant such as (Lycopersicon esculentum), the R3 iteron has carried out amino acid whose sequence multiple comparisons (seeing accompanying drawing 6), and the R2R3 tumor-necrosis factor glycoproteins has higher consistence in these species as can be seen.
Systematic evolution tree analysis revealed (seeing accompanying drawing 7) based on aminoacid sequence, the myb gene genetic variation and genetic differentiation of different plants is very big, myb gene of broom corn millet and the similarity degree of paddy rice are the highest, are both monocotyledons, and the myb gene similarity of barley and corn and broom corn millet has only 46% and 41% respectively.
3. the RT-PCR of broom corn millet drought-resisting regulating gene MYB transcription factor gene expression pattern analyzes
Synthesized a pair of amplification pearl millet (Pennisetum glaucum (L.) R.Br according to document, pearlmillet) primer of the Actin muscle of constitutive expression (actin) gene fragment, mRNA to broom corn millet carries out the RT-PCR amplification, obtain the product of a 171bp, sequence alignment after the order-checking shows, this fragment and rice actin (actin) have higher homology, and the fragment of acquisition is a broom corn millet actin gene fragment, determine the template amount of different treatment as confidential reference items with this gene.
Employing is according to the primer (5-CTGGTGACCTATTGAGCCC-3 and 5-CGGCAGAAAGGCATTGAC-3) of this broom corn millet MYB class transcription factor gene sequences Design, to broom corn millet drought-resisting germplasm materials plant respectively soil relative water content be 75%, 36%, 24% and rehydration after the mRNA that extracts of the leaf sample of 2h, 6h, 12h carry out RT-PCR and analyze.
Comprise in the 20 μ L reaction systems: the cDNA template of the appropriate volume of adjusting through confidential reference items, 10 * PCRBuffer, 2 μ L, dNTP (2mmol/L) 2 μ L, forward primer (10mmol/L) 0.5 μ L, reverse primer (10mmol/L) 0.5 μ L, Taq DNA polymerase (5U/ μ L) 0.2 μ L, ddH
2O polishing to 20 μ L.
The PCR response procedures is: 95 ℃ of pre-sex change 3min, then carry out 95 ℃ of 40s, 55 ℃ of 45s, 72 ℃ of 40s, totally 35 circulations; 72 ℃ are extended 8min, and the reaction back is in 4 ℃ of insulations.1.5% agarose gel electrophoresis detects amplified production.
RT-PCR expression pattern analysis revealed (seeing accompanying drawing 8), this broom corn millet drought-resisting regulating gene MYB transcription factor gene under normal circumstances has a certain amount of expression, (soil relative water content is 36%) abduction delivering under drought stress, severe is coerced down (soil relative water content 24%) expression and is suppressed, the 2h expression amount is the highest after the rehydration, drops to the level also lower than normal growth subsequently.Illustrate that this gene mainly is subjected to the inducing of signal at drought stress initial stage and rehydration initial stage, thus the expression of regulation and control and arid defence and the quick relevant functional gene of growing of rehydration.
Broom corn millet drought-resisting regulating gene MYB transcription factor gene of the present invention derives from the gramineous crop broom corn millet of drought resisting, for the drought resistance and the water use efficiency of improvement gramineous crop provides better choice.And this gene belongs to regulatory gene, can start the expression of downstream series of genes, is more convenient for being applied to the improvement of plant drought resistance and water use efficiency, improves drought resistance and the water use efficiency of plant effectively.
Below be the embodiment that the contriver provides:
Embodiment 1:
Be connected with the expression vector that contains corresponding plant promoter (as CaMV 35 etc.) with this gene, make up the expression vector of broom corn millet drought-resisting regulating gene MYB transcription factor gene, acquisitions such as transformed into escherichia coli contain the bacterial strain of this gene; Perhaps this gene is connected with T-DNA binary vector that contains corresponding plant promoter (as CaMV 35 etc.) etc., make up the T-DNA expression vector of broom corn millet drought-resisting regulating gene MYB transcription factor gene, acquisitions such as transformed into escherichia coli contain the bacterial strain of this gene, with corresponding Agrobacterium strain is to merge, and obtains to contain the agrobacterium strains of this gene.
Embodiment 2:
The coli strain or the agrobacterium strains that obtain among the embodiment 1 are bred, extraction contains the DNA or the direct bacterium liquid that utilizes breeding to obtain of the expression vector of broom corn millet drought-resisting regulating gene MYB transcription factor gene, method (as pollen tube passage method, particle bombardment, agrobacterium-mediated transformation etc.) transformed wheat, seeding corn and other crops or other plant of adopting this area staff to know altogether; Screening obtains the appropriate transfer-gen plant of expressing of this gene, the drought resistance and the water use efficiency of existing crop of improvement or plant variety.
Sequence table
The genomic dna sequence of broom corn millet drought-resisting regulating gene MYB transcription factor gene and cDNA sequence gene group dna sequence dna:
AGTTGCTGCCGTTCTCCCACAAGTTCTACTCTATCAGACACCACTATATGAGCCCATATA 60
AGCAGTTCGTCCACAATAATCCCACCCTTAAGCCATAAATTGCTGAGAGGTGTTCTTAGA 120
ACCAGAGGATTACCTGCAGAGTTCTGTGCTGCTCCCCAAGGTGTGAGCTGTGCAGATTGC 180
AGAGCTCAGCTTCAGCTTCTGCATTGCTTTCAATGGGGAGGCACTCCTGCTGTTACAAGC 240
CCAAACATGGGCATGGCTGCTGGAGCACTGTCCCAAAGCTTGCAGGTATAATCAAAATCC 360
TGCACACTTTATTCTTCTCCATAATCAATTTCCACTGCTTCGTGTTCCTCTCCAAGGCAA 420
CTTCTTTCTCCCAGTCTTCTCATAACGGCCCAATTAATGGTTGCAGGGCTTCAGAGATGT 480
GGAAAGAGCCGCAGGCTGAGATGGATAAATTACCTGAGGCCTGACCTCAAAAGAGGTGCA 540
ATTTTATCCCAAGTTGCACTGTCAGAATTTTGCACCAATTGCTTTAAAGCAAGCTTTCCT 660
TAACTATGCCTTTTTCACGTAAATGACAGGTGGTCTCAGATTGCAACACGGTTGCCTGGA 720
AGAACTGATAACGAGATCAAGAATCTCTGGAACTCAAGCATCAAGAAGAAGCTCCGGCAG 780
AAAGGCATTGACCCCAACACCTACAAGCCCCTTGCCGAGGTTTTTCGCAAAGCAGCTCCC 840
CTTGGCAACTTGAGCCATATTCTCAGTGAGACAGCACAATCACCAGAGCTGCTGCCAGTG 960
CCAATCCCAAATTTCCCTTTCCAGCAGTTGATGTGCTACAGCAACGAACTTGGCAGCAAG 1140
CATGGTGGCAGCACGAATTACCTCTGGTTTAACCAGAATGAGTCAAGCTGCAGCACCGTT 1200
ACGACTAATCCTAGCAGCAGCAGCAGTAAAGGAAGCAGTGGAAGCAACAGCTTGGGATTC 1380
GAGCTGCAAAGCACAAGCTCAATTCTGGAGAATAGTATCTTCCCATGGACAGATTTATCA 1440
TTTACAGATACACCAGCAACCATGCAGAATCTTAGCCAATCACTGTATGAAGATGTGGTC 1560
AAAGCCGAGAGCCAATTCAACATGGAGGGCCTCTGTGCCGCTTGGTCTCAAAATCTGCAG 1620
CCACAGCAACATCTGCAGGTAGTATCCGATTTGTATGACAAGGATTTGCAGAGAATGTCC 1680
TTGTCTTTTGAGAATATCTAGGCCACATGTTTCAGCATGAAGGGAACAGAGCACCAGAT 1740
The cDNA sequence of gene:
AGCAGTTCGTCCACAATAATCCCACCCTTAAGCCATAAATTGCTGAGAGGTGTTCTTAGA 120
ACCAGAGGATTACCTGCAGAGTTCTGTGCTGCTCCCCAAGGTGTGAGCTGTGCAGATTGC 180
CCAAACATGGGCATGGCTGCTGGAGCACTGTCCCAAAGCTTGCAGGGCTTCAGAGATGTG 360
GAAAGAGCCGCAGGCTGAGATGGATAAATTACCTGAGGCCTGACCTCAAAAGAGGTGCAT 420
CTCAGATTGCAACACGGTTGCCTGGAAGAACTGATAACGAGATCAAGAATCTCTGGAACT 540
CCGAGGTTTTTCGCAAAGCAGCTCCCACAATCAGTACTGAAAGAACCTCCGAGTCCAGTG 660
CACAATCACCAGAGCTGCTGCCAGTGCTCGGTAAGCATCGCAAAGAAACTACTAGTTTGG 780
CGCATCTAAGGGTGCCACCGAAGGAGCTATTCCTTGATCAGCTTGTTTCTAGTCATGATA 840
GCTACAGCAACGAACTTGGCAGCAAGCATGGTGGCAGCACGAATTACCTCTGGTTTAACC 960
TTCAGAGTACCCAATTCTATTGGGATACGACTAATCCTAGCAGCAGCAGCAGTAAAGGAA 1140
GCAGTGGAAGCAACAGCTTGGGATTCGAGCTGCAAAGCACAAGCTCAATTCTGGAGAATA 1200
GCCAATCACTGTATGAAGATGTGGTCAAAGCCGAGAGCCAATTCAACATGGAGGGCCTCT 1380
GTGCCGCTTGGTCTCAAAATCTGCAGCCACAGCAACATCTGCAGGTAGTATCCGATTTGT 1440
GCATGAAGGGAACAGAGCACCAGAT 1560
Claims (3)
1. broom corn millet drought-resisting regulating gene MYB transcription factor gene, it is characterized in that: the genomic dna sequence of this gene and cDNA sequence are respectively:
(1) the genome sequence total length is 1739bp, comprises two introns of 121bp and 93bp, three exons, and its dna sequence dna is:
AGTTGCTGCCGTTCTCCCACAAGTTCTACTCTATCAGACACCACTATATGAGCCCATATA 60
AGCAGTTCGTCCACAATAATCCCACCCTTAAGCCATAAATTGCTGAGAGGTGTTCTTAGA 120
ACCAGAGGATTACCTGCAGAGTTCTGTGCTGCTCCCCAAGGTGTGAGCTGTGCAGATTGC 180
AGAGCTCAGCTTCAGCTTCTGCATTGCTTTCAATGGGGAGGCACTCCTGCTGTTACAAGC 240
AGAAGCTGAGGAAGGGGCTCTGGTCTCCTGAGGAAGATGAGAAGCTCATGAACCACATAA 300
CCAAACATGGGCATGGCTGCTGGAGCACTGTCCCAAAGCTTGCAGGTATAATCAAAATCC 360
TGCACACTTTATTCTTCTCCATAATCAATTTCCACTGCTTCGTGTTCCTCTCCAAGGCAA 420
CTTCTTTCTCCCAGTCTTCTCATAACGGCCCAATTAATGGTTGCAGGGCTTCAGAGATGT 480
GGAAAGAGCCGCAGGCTGAGATGGATAAATTACCTGAGGCCTGACCTCAAAAGAGGTGCA 540
TTCTCTCAGGAAGAGGAAGACCTTATCATTGAACTCCATGCTGTTTTGGGCAACAGGTAA 600
ATTTTATCCCAAGTTGCACTGTCAGAATTTTGCACCAATTGCTTTAAAGCAAGCTTTCCT 660
TAACTATGCCTTTTTCACGTAAATGACAGGTGGTCTCAGATTGCAACACGGTTGCCTGGA 720
AGAACTGATAACGAGATCAAGAATCTCTGGAACTCAAGCATCAAGAAGAAGCTCCGGCAG 780
AAAGGCATTGACCCCAACACCTACAAGCCCCTTGCCGAGGTTTTTCGCAAAGCAGCTCCC 840
ACAATCAGTACTGAAAGAACCTCCGAGTCCAGTGATGTTGACCCTTCAAGTGGTGGCGCA 900
CTTGGCAACTTGAGCCATATTCTCAGTGAGACAGCACAATCACCAGAGCTGCTGCCAGTG 960
CTCGGTAAGCATCGCAAAGAAACTACTAGTTTGGCGCATCTAAGGGTGCCACCGAAGGAG 1020
CTATTCCTTGATCAGCTTGTTTCTAGTCATGATAACCTGCCCGGCTGCCGCTCAACTGGC 1080
CCAATCCCAAATTTCCCTTTCCAGCAGTTGATGTGCTACAGCAACGAACTTGGCAGCAAG 1140
CATGGTGGCAGCACGAATTACCTCTGGTTTAACCAGAATGAGTCAAGCTGCAGCACCGTT 1200
TCCACTGTGATGGCACCAGTTTCGCCGTCAACTCTCTCAACATCAACAGGGCTCAATAGG 1260
TCACCAGAGAATCCACACTCTGGAGGTACTGGCATTCAGAGTACCCAATTCTATTGGGAT 1320
ACGACTAATCCTAGCAGCAGCAGCAGTAAAGGAAGCAGTGGAAGCAACAGCTTGGGATTC 1380
GAGCTGCAAAGCACAAGCTCAATTCTGGAGAATAGTATCTTCCCATGGACAGATTTATCA 1440
CCAGATAAAAATAGCCACCTAGAGGAAGAACTCAAGTGGCCTGACTTGCTCCATGGAACC 1500
TTTACAGATACACCAGCAACCATGCAGAATCTTAGCCAATCACTGTATGAAGATGTGGTC 1560
AAAGCCGAGAGCCAATTCAACATGGAGGGCCTCTGTGCCGCTTGGTCTCAAAATCTGCAG 1620
CCACAGCAACATCTGCAGGTAGTATCCGATTTGTATGACAAGGATTTGCAGAGAATGTCC 1680
TTGTCTTTTGAGAATATCTAGGCCACATGTTTCAGCATGAAGGGAACAGAGCACCAGAT 1740
(2) cDNA sequence total length is 1525bp, and wherein 3 ' non-translational region is 212bp, and 5 ' non-translational region is 41bp, and the coding region is 1272bp, and there are the abundant district of a Serine in 424 amino acid of encoding altogether, C-end; The cDNA sequence of this gene is:
AGTTGCTGCCGTTCTCCCACAAGTTCTACTCTATCAGACACCACTATATGAGCCCATATA 60
AGCAGTTCGTCCACAATAATCCCACCCTTAAGCCATAAATTGCTGAGAGGTGTTCTTAGA 120
ACCAGAGGATTACCTGCAGAGTTCTGTGCTGCTCCCCAAGGTGTGAGCTGTGCAGATTGC 180
AGAGCTCAGCTTCAGCTTCTGCATTGCTTTCAATGGGGAGGCACTCCTGCTGTTACAAGC 240
AGAAGCTGAGGAAGGGGCTCTGGTCTCCTGAGGAAGATGAGAAGCTCATGAACCACATAA 300
CCAAACATGGGCATGGCTGCTGGAGCACTGTCCCAAAGCTTGCAGGGCTTCAGAGATGTG 360
GAAAGAGCCGCAGGCTGAGATGGATAAATTACCTGAGGCCTGACCTCAAAAGAGGTGCAT 420
TCTCTCAGGAAGAGGAAGACCTTATCATTGAACTCCATGCTGTTTTGGGCAACAGGTGGT 480
CTCAGATTGCAACACGGTTGCCTGGAAGAACTGATAACGAGATCAAGAATCTCTGGAACT 540
CAAGCATCAAGAAGAAGCTCCGGCAGAAAGGCATTGACCCCAACACCTACAAGCCCCTTG 600
CCGAGGTTTTTCGCAAAGCAGCTCCCACAATCAGTACTGAAAGAACCTCCGAGTCCAGTG 660
ATGTTGACCCTTCAAGTGGTGGCGCACTTGGCAACTTGAGCCATATTCTCAGTGAGACAG 720
CACAATCACCAGAGCTGCTGCCAGTGCTCGGTAAGCATCGCAAAGAAACTACTAGTTTGG 780
CGCATCTAAGGGTGCCACCGAAGGAGCTATTCCTTGATCAGCTTGTTTCTAGTCATGATA 840
ACCTGCCCGGCTGCCGCTCAACTGGCCCAATCCCAAATTTCCCTTTCCAGCAGTTGATGT 900
GCTACAGCAACGAACTTGGCAGCAAGCATGGTGGCAGCACGAATTACCTCTGGTTTAACC 960
AGAATGAGTCAAGCTGCAGCACCGTTTCCACTGTGATGGCACCAGTTTCGCCGTCAACTC 1020
TCTCAACATCAACAGGGCTCAATAGGTCACCAGAGAATCCACACTCTGGAGGTACTGGCA 1080
TTCAGAGTACCCAATTCTATTGGGATACGACTAATCCTAGCAGCAGCAGCAGTAAAGGAA 1140
GCAGTGGAAGCAACAGCTTGGGATTCGAGCTGCAAAGCACAAGCTCAATTCTGGAGAATA 1200
GTATCTTCCCATGGACAGATTTATCACCAGATAAAAATAGCCACCTAGAGGAAGAACTCA 1260
AGTGGCCTGACTTGCTCCATGGAACCTTTACAGATACACCAGCAACCATGCAGAATCTTA 1320
GCCAATCACTGTATGAAGATGTGGTCAAAGCCGAGAGCCAATTCAACATGGAGGGCCTCT 1380
GTGCCGCTTGGTCTCAAAATCTGCAGCCACAGCAACATCTGCAGGTAGTATCCGATTTGT 1440
ATGACAAGGATTTGCAGAGAATGTCCTTGTCTTTTGAGAATATCTAGGCCACATGTTTCA 1500
GCATGAAGGGAACAGAGCACCAGAT。1560
2. the described broom corn millet drought-resisting regulating gene MYB transcription factor gene of claim 1, be used for being connected, make up the purposes of the host bacterium of the expression vector of broom corn millet MYB class transcription factor gene or the broom corn millet MYB class transcription factor gene that conversion obtains with corresponding plant promoter.
3. purposes as claimed in claim 2, it is characterized in that, by methods such as particle gun, agriculture bacillus mediated or pollen tube channels the expression vector of broom corn millet drought-resisting regulating gene MYB transcription factor gene is imported wheat, seeding corn and other crops or other plant, transgenic plant cells system and transfer-gen plant that the drought resistance of acquisition and water use efficiency improve.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101928337A (en) * | 2010-08-18 | 2010-12-29 | 北京林业大学 | Chrysanthemum drought-tolerant gene and application thereof |
| CN109456982A (en) * | 2018-11-29 | 2019-03-12 | 周口师范学院 | The application of rice Os MYB6 gene and its coding albumen in drought resisting and salt resistance |
| CN109666679A (en) * | 2019-03-01 | 2019-04-23 | 中国农业大学 | Chinese rose transcription factor RhPTM and its application |
| CN117209583A (en) * | 2023-11-09 | 2023-12-12 | 吉林农业大学 | Application of gene ZmMYB86 in improving drought resistance of plants |
-
2006
- 2006-08-07 CN CN 200610104478 patent/CN1908172A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101928337A (en) * | 2010-08-18 | 2010-12-29 | 北京林业大学 | Chrysanthemum drought-tolerant gene and application thereof |
| CN101928337B (en) * | 2010-08-18 | 2011-12-14 | 北京林业大学 | Chrysanthemum drought-tolerant gene and application thereof |
| CN109456982A (en) * | 2018-11-29 | 2019-03-12 | 周口师范学院 | The application of rice Os MYB6 gene and its coding albumen in drought resisting and salt resistance |
| CN109456982B (en) * | 2018-11-29 | 2022-03-04 | 周口师范学院 | Application of rice OsMYB6 gene and encoding protein thereof in drought resistance and salt resistance |
| CN109666679A (en) * | 2019-03-01 | 2019-04-23 | 中国农业大学 | Chinese rose transcription factor RhPTM and its application |
| CN117209583A (en) * | 2023-11-09 | 2023-12-12 | 吉林农业大学 | Application of gene ZmMYB86 in improving drought resistance of plants |
| CN117209583B (en) * | 2023-11-09 | 2024-03-22 | 吉林农业大学 | Application of gene ZmMYB86 in improving drought resistance of plants |
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