CN1907017A - Production method for cultureing organism and special equipment thereof - Google Patents
Production method for cultureing organism and special equipment thereof Download PDFInfo
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- CN1907017A CN1907017A CN 200610112420 CN200610112420A CN1907017A CN 1907017 A CN1907017 A CN 1907017A CN 200610112420 CN200610112420 CN 200610112420 CN 200610112420 A CN200610112420 A CN 200610112420A CN 1907017 A CN1907017 A CN 1907017A
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- climatic cabinate
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- 238000004519 manufacturing process Methods 0.000 title description 3
- 238000000034 method Methods 0.000 claims abstract description 10
- 241001465754 Metazoa Species 0.000 claims description 11
- 239000003638 chemical reducing agent Substances 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims description 2
- 210000000056 organ Anatomy 0.000 claims description 2
- 239000007789 gas Substances 0.000 abstract description 83
- 229910000831 Steel Inorganic materials 0.000 abstract description 6
- 239000010959 steel Substances 0.000 abstract description 6
- 241000196324 Embryophyta Species 0.000 description 12
- 238000005286 illumination Methods 0.000 description 7
- 241000219194 Arabidopsis Species 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 230000000630 rising effect Effects 0.000 description 5
- 230000033228 biological regulation Effects 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 238000010170 biological method Methods 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- MPDGHEJMBKOTSU-YKLVYJNSSA-N 18beta-glycyrrhetic acid Chemical compound C([C@H]1C2=CC(=O)[C@H]34)[C@@](C)(C(O)=O)CC[C@]1(C)CC[C@@]2(C)[C@]4(C)CC[C@@H]1[C@]3(C)CC[C@H](O)C1(C)C MPDGHEJMBKOTSU-YKLVYJNSSA-N 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000009975 flexible effect Effects 0.000 description 1
- 239000002803 fossil fuel Substances 0.000 description 1
- 239000005431 greenhouse gas Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/36—Means for collection or storage of gas; Gas holders
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Cultivation Of Plants (AREA)
- Greenhouses (AREA)
Abstract
The invention discloses a biology cultivating method and specific equipment, which comprises the following parts: artificial climate box bulk, at least one gas sensor in the bulk and gas steel bottle to reserve corresponding gas, wherein the gas steel bottle contains gas transmitting pipe with electromagnetic valve, which connects the inner part of artificial box bulk; the gas sensor connects electromagnetic valve through relay. The equipment can control density of multiple gases in the box bulk, which reduces cost effectively.
Description
Technical field
The present invention relates to a kind of cultivate biological method and special equipment thereof.
Background technology
Owing to a large amount of consumption of fossil fuel, the considerable damage of vegetation and the reasons such as a large amount of felling of forest, various violent variations have taken place in natural environment, comprising the CO in the atmosphere
2, SO
2, NO, CH
4, O
3Constantly rise etc. various greenhouse gases or harmful gas concentration, the rising of these gas concentrations has caused a series of biology and Ecology Action, and causes various countries scientist's common concern.Usually, the scientist of association area is by the following atmospheric environment condition of manual simulation, study the influence degree of the rising of these gas concentrations, so that be that the contingent variation of the ecosystem provides scientific forecasting under the situation that all gases concentration raises in the following atmosphere to natural environment.
As with CO in the atmosphere
2Concentration raises to example, expects the end of this century, the CO in the atmosphere
2Concentration will be increased to 540-970 μ molmol
-1, Atmospheric CO
2The concentration caused Global climate change that raises has been subjected to extensive concern.The various countries scientist who is engaged in this area research is studying the Global Ecological system in following Atmospheric CO
2Will how to change under the concentration rising situation.Normally used method is the artificial experimental system of making, and increases the CO in this system
2Concentration is to simulate following Atmospheric CO
2Environmental condition under the concentration rising situation places experiment material this system then, and the variation of observation experiment material in this simulation system is so that be following Atmospheric CO
2Under the concentration rising situation, the contingent variation of the ecosystem provides prediction.The experimental system that is adopted comprises a variety of, as being experimental provision with the climatic cabinate, with the CO of box house
2Concentration is elevated to the following concentration of being predicted, carries out related experiment then.
The climatic cabinate supply that can control multiple gases concentration is seldom arranged in the market, even certain gas of control such as CO are arranged
2The climate box supply of concentration, its price are also very high, and the 4-5 of normally common climatic cabinate price doubly.Therefore, the climatic cabinate that can control multiple gases concentration will have vast market prospect.
Summary of the invention
The cultural method and the special equipment thereof that the purpose of this invention is to provide a kind of biology.
The equipment that is used to cultivate biology provided by the present invention, the called after bio-incubator, it comprises the climatic cabinate body, and it also comprises at least one gas sensor that is located at described climatic cabinate body interior and stores the gas bomb of the corresponding gas of experiencing with described gas sensor; Described gas bomb communicates with described climatic cabinate body interior by the gas pipeline that is provided with magnetic valve; Described gas sensor links to each other with described magnetic valve by relay.
Described climatic cabinate body adopts the climatic cabinate of commercially available biological field to get final product, as the climatic cabinate of manufacturer production such as east, Harbin connection electronic technology development corporation, Ltd., Jintan City, Jiangsu Province Medical Instruments factory or Chongqing City immortality experimental instrument factory.
The quantity of described gas sensor and gas bomb can be according to actual needs (as size of climatic cabinate body etc.) be made as one or more so that the gas of the required regulation and control concentration of box house evenly distributes.
Described relay links to each other with relay by sensor signal lines, and described magnetic valve links to each other with relay by control line.
The gas outlet of described gas bomb also is provided with gas pressure reducer.
Described relay and gas bomb are positioned at the climatic cabinate body exterior.
The said equipment is to design on the basis of climatic cabinate, its objective is that realization regulates and control the multiple gases concentration in the casing, and described gas can be CO
2, SO
2, NO, CH
4Or O
3Deng; When the gas of required regulation and control concentration is CO
2The time, described gas sensor is CO
2Gas in the sensor, described gas bomb is CO
2When the gas of required regulation and control concentration is O
2, SO
2, NO, CH
4Or O
3Deng CO
2During in addition other gas, described gas sensor is changed to the sensor of corresponding gas, and the gas in the described gas bomb is changed to corresponding gas and gets final product.
In addition, evenly distribute,, can a fan respectively be installed at the place, wherein two diagonal angles of box house to prevent local gas excessive concentration or low excessively for making the gas concentration in the casing.
Second purpose of the present invention provides a kind of biological method of cultivating.
Method of cultivating biology provided by the present invention is to treat the cultivation biology with the said equipment to cultivate.
Above-mentioned cultural method all is suitable for various biologies, comprises plant or its portion of tissue and organ (as seed, bud, cutting, callus or suspension cell etc.), microorganism and animal etc.
The invention provides a kind of biological culturing method and special equipment thereof.This equipment is to transform to form on the basis of common climatic cabinate, in this equipment, the effect of gas sensor is a concentration of waiting gas in the induction casing, and give relay by electrical signal transfer with it, relay is transformed into data signal with this signal of telecommunication, on relay, can demonstrate box house and wait the concentration numerical value of gas, if detected concentration value is higher than predefined numerical value, relay will spread out of the signal of cutting out magnetic valve so, thereby gas bomb is stopped to the casing supply gas, vice versa, thereby the gas concentration of waiting of box house is fluctuateed near setting value in advance always; CO is installed
2The purpose of pressure-reducing valve is that speed is too fast and can't accurately control the concentration of box house when preventing that gas in the steel cylinder from entering climatic cabinate, also can avoid the gas distillation simultaneously and the steel cylinder gas outlet is freezing.This equipment can also more accurately be controlled the box house multiple gases (as CO except can accurately controlling temperature, humidity and the intensity of illumination in the casing
2, SO
2, NO, CH
4Or O
3Deng) concentration, and its preparation cost only is common CO
2About 30% of climatic cabinate.Simple, with low cost, the flexible property of equipment preparation method of the present invention is good, can be used for the cultivation of animal and plant, has higher economic benefits and wide application prospect.
Below in conjunction with specific embodiment the present invention is described in further detail.
Description of drawings
Fig. 1 for the structural representation of biological culture special equipment of the present invention (with CO
2Be example)
Embodiment
Following embodiment method therefor is conventional method if no special instructions.
One, CO
2The acquisition of bio-incubator
With CO
2Be example, the structural representation of bio-incubator of the present invention as shown in Figure 1, it comprises climatic cabinate body 1, it also comprises a CO who is located at described climatic cabinate body interior
2(this sensor detects CO to gas sensor 2
2Concentration range be 0-3000 μ mo1mol
-1, error rate is ± 1.6%) and CO
2Gas bomb 3; Described CO
2Gas bomb communicates with described climatic cabinate body interior by the gas pipeline 5 that is provided with magnetic valve 4; Described gas sensor links to each other with described magnetic valve by relay 6; Described relay links to each other with relay by sensor signal lines 7, and described magnetic valve links to each other with relay by control line 8; Described CO
2The gas outlet of gas bomb also is provided with a pressure-reducing valve 9.Described relay and gas bomb are positioned at the climatic cabinate body exterior.
In addition, evenly distribute, at the place, wherein two diagonal angles of box house a fan 10 is installed respectively again for making the gas concentration in the casing.
The operation principle of bio-incubator of the present invention is: at first according to needed CO in the climatic cabinate
2Concentration preestablishes respective value on relay, then, and the CO of box house
2Sensor can will detect CO
2The electrical signal transfer of concentration is given relay, and relay converts the electrical signal to data signal, and this data signal is exactly the CO of detected box house
2Concentration value, if detected concentration value is higher than predefined numerical value, relay will spread out of the signal of cutting out magnetic valve so, thereby makes CO
2Gas bomb stops to casing supply CO
2Gas, vice versa, thereby can make the CO of box house
2Concentration fluctuates near setting value in advance always.In addition, CO is installed
2The purpose of pressure-reducing valve is the CO that prevents in the steel cylinder
2Speed can't accurately be controlled the concentration of box house too soon when gas entered climatic cabinate, also can avoid CO simultaneously
2The distillation of gas is freezing with the steel cylinder gas outlet.
Two, the cultivation of plant
With the arabidopsis is example, the bio-incubator that obtains with embodiment 1 cultivates plants, and concrete grammar is: after according to a conventional method the arabidopsis seed being carried out vernalization, with planting seed in common commercially available culture matrix, be placed in the bio-incubator then and cultivate, with the CO in the casing
2Gas concentration is controlled at 700 μ molmol
-1Near, regulate the envirment factor controller of artificial climate incubator simultaneously, making the photoperiod is illumination in 16 hours, 8 hours dark; Light intensity is 350 μ molm
-2s
-1Temperature under the illumination condition is 23 ℃, and relative moisture is 80%; Temperature under the dark condition is 19 ℃, and relative moisture is greater than 90%.
As a result, after planting about 40 days, plant blossom, seed begins maturation in 60 days relief angle fruits, about 100 days of whole life cycle, proves that bio-incubator of the present invention can be used for the cultivation of plant.
Three, the breed of animal
With the small white mouse is example, and with the bio-incubator cultivated animals that embodiment 1 obtains, concrete grammar is: place bio-incubator to culture Kunming, Yunnan kind small white mouse, with the CO in the casing
2Gas concentration is controlled at 800 μ molm
-2s
-1Near, regulate the envirment factor controller of artificial climate incubator simultaneously, making the photoperiod is that (light intensity is 100 μ molm in illumination in 12 hours
-2s
-1), 12 hours dark, temperature are 25 ℃, on time feedwater, feeding.
Mouse growing state in casing is good as a result, can normally produce offspring, and proves that bio-incubator of the present invention also can be used for the breed of animal.
One, O
3The acquisition of bio-incubator
O of the present invention
3The CO of the structure of bio-incubator and embodiment 1
2Bio-incubator is basic identical, and it comprises the climatic cabinate body, and it also comprises an O who is located at described climatic cabinate body interior
3(this sensor detects O to gas sensor
3Concentration range be 0-1000nmolmol
-1, error rate is ± 10%) and O
3Gas bomb; Described O
3Gas bomb communicates with described climatic cabinate body interior by the gas pipeline that is provided with magnetic valve; Described gas sensor links to each other with described magnetic valve by relay; Described relay links to each other with relay by sensor signal lines, and described magnetic valve links to each other with relay by control line; Described O
3The gas outlet of gas bomb also is provided with a pressure-reducing valve.Described relay and gas bomb are positioned at the climatic cabinate body exterior.
Described relay and gas bomb are positioned at the climatic cabinate body exterior.
In addition, evenly distribute, at the place, wherein two diagonal angles of box house a fan is installed respectively again for making the gas concentration in the casing.
This O
3The CO of the operation principle of bio-incubator and embodiment 1
2Bio-incubator is identical.
Two, the cultivation of plant
With the arabidopsis is example, the bio-incubator that obtains with embodiment 2 cultivates plants, and concrete grammar is: after according to a conventional method the arabidopsis seed being carried out vernalization, with planting seed in common commercially available culture matrix, be placed in the bio-incubator then and cultivate, with the O in the casing
3Gas concentration is controlled at 60nmolmol
-1Near, regulate the envirment factor controller of artificial climate incubator simultaneously, making the photoperiod is illumination in 16 hours, 8 hours dark; Light intensity is 350 μ molm
-2s
-1Temperature under the illumination condition is 23 ℃, and relative moisture is 80%; Temperature under the dark condition is 19 ℃, and relative moisture is greater than 90%.
Found that arabidopsis is at O
3Growing under the environmental condition had a strong impact on, mainly show as leaf photosynthesis and chlorophyll content significantly descends, plant obviously diminishes, biomass significantly descends, flower amount and seed production also significantly reduce, experimental result conforms to expected results, proves that bio-incubator of the present invention can be used for detecting different O
3The upgrowth situation of each plant species under the gas concentration.
Three, the breed of animal
With the small white mouse is example, and with the bio-incubator cultivated animals that embodiment 1 obtains, concrete grammar is: place bio-incubator to culture Kunming, Yunnan kind small white mouse, with the O in the casing
3Gas concentration is controlled at 150nmolmol
-1Near, regulate the envirment factor controller of artificial climate incubator simultaneously, making the photoperiod is that (light intensity is 100 μ molm in illumination in 12 hours
-2s
-1), 12 hours dark, temperature are 25 ℃, on time feedwater, feeding.
As a result small white mouse in casing, grow slack-off, lather, immunocompetence significantly descends, reproduction ability significantly descends, and conforms to expected results, proves that bio-incubator of the present invention also can be used for detecting different O
3The upgrowth situation of various animals under the gas concentration.
Claims (10)
1, be used to cultivate biological equipment, it comprises the climatic cabinate body, it is characterized in that: it also comprises at least one gas sensor that is located at described climatic cabinate body interior and stores the gas bomb of the corresponding gas of experiencing with described gas sensor; Described gas bomb communicates with described climatic cabinate body interior by the gas pipeline that is provided with magnetic valve; Described gas sensor links to each other with described magnetic valve by relay.
2, equipment according to claim 1 is characterized in that: described relay links to each other with relay by sensor signal lines, and described magnetic valve links to each other with relay by control line.
3, equipment according to claim 1 is characterized in that: the gas outlet of described gas bomb is provided with gas pressure reducer.
4, equipment according to claim 1 is characterized in that: the quantity of described gas sensor and gas bomb is one.
5, equipment according to claim 1 is characterized in that: described relay and gas bomb are positioned at the climatic cabinate body exterior.
6, equipment according to claim 1 is characterized in that: described gas sensor is CO
2Gas in the sensor, gas bomb is CO
2
7, equipment according to claim 1 is characterized in that: described gas sensor is O
2Gas in the sensor, gas bomb is O
2
8, equipment according to claim 1 is characterized in that: a fan is respectively installed at the place, wherein two diagonal angles in described climatic cabinate body interior.
9, a kind of method of cultivating biology is to treat the cultivation biology with each described equipment of claim 1-8 to cultivate.
10, method according to claim 9 is characterized in that: described biology comprises plant or its portion of tissue, organ, microorganism and animal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610112420 CN1907017A (en) | 2006-08-16 | 2006-08-16 | Production method for cultureing organism and special equipment thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610112420 CN1907017A (en) | 2006-08-16 | 2006-08-16 | Production method for cultureing organism and special equipment thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1907017A true CN1907017A (en) | 2007-02-07 |
Family
ID=37698387
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610112420 Pending CN1907017A (en) | 2006-08-16 | 2006-08-16 | Production method for cultureing organism and special equipment thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1907017A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102553663A (en) * | 2011-12-16 | 2012-07-11 | 北京航空航天大学 | Artificial climate box capable of simulating multiple complex environment conditions |
| CN104535752A (en) * | 2014-12-19 | 2015-04-22 | 环境保护部南京环境科学研究所 | Environmental factor-biological interaction simulation device for terrestrial ecological system |
| CN105886394A (en) * | 2014-10-15 | 2016-08-24 | 中国航天员科研训练中心 | Apparatus and method for microalgae culture experiment in low pressure environment |
-
2006
- 2006-08-16 CN CN 200610112420 patent/CN1907017A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102553663A (en) * | 2011-12-16 | 2012-07-11 | 北京航空航天大学 | Artificial climate box capable of simulating multiple complex environment conditions |
| CN102553663B (en) * | 2011-12-16 | 2014-02-26 | 北京航空航天大学 | Artificial climate box capable of simulating multiple complex environment conditions |
| CN105886394A (en) * | 2014-10-15 | 2016-08-24 | 中国航天员科研训练中心 | Apparatus and method for microalgae culture experiment in low pressure environment |
| CN104535752A (en) * | 2014-12-19 | 2015-04-22 | 环境保护部南京环境科学研究所 | Environmental factor-biological interaction simulation device for terrestrial ecological system |
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