CN1899982A - Novel strain capable of decomposing tetramethyl ammonium hydroxide and method for treating waste water using the strain - Google Patents

Novel strain capable of decomposing tetramethyl ammonium hydroxide and method for treating waste water using the strain Download PDF

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CN1899982A
CN1899982A CNA200510118775XA CN200510118775A CN1899982A CN 1899982 A CN1899982 A CN 1899982A CN A200510118775X A CNA200510118775X A CN A200510118775XA CN 200510118775 A CN200510118775 A CN 200510118775A CN 1899982 A CN1899982 A CN 1899982A
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ibn
tmah
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廉焘荣
丁相允
梁在均
具本琸
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IN BIONET Inc
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Abstract

The present invention relates to a novel strain capable of decomposing tetramethyl ammonium hydroxide and method for treating waste water using the strain. More specifically, the present invention relates to a novel strain capable of effectively decomposing TMAH which has toxicity and is hard to be decomposed and is largely contained in the exhaust liquid of the photoelectronic industry and to a method for treating waste water using the strain in the procedure of a membrane bio-reactor maintaining high microbiology concentration. The strain and the waste water treatment method of the present invention is capable of, in relation to the former apparatus, minimizing the occupation of land, reducing the equipment cost and the running cost, so that the method can be widely used in industry situ as Environment Amity Type waste water treatment method.

Description

The method of wastewater treatment that can decompose the novel strain of tetramethyl ammonium hydroxide and utilize described bacterial strain
Technical field
The present invention relates to the method for wastewater treatment that can decompose the novel strain of tetramethyl ammonium hydroxide and utilize described bacterial strain.More particularly, the present invention relates to can be to containing and has novel strain that hard-decomposed and toxic TMAH effectively decomposes and the method for wastewater treatment that utilizes described bacterial strain in a large number in the waste liquid of photoelectronic industry.
Background technology
Photoelectron (opto-electronic) industry is worldwide positive recently fast-developing sophisticated technology industry.Because the human consumer sharply increases the demand of photoelectronic industry product, a lot of countries Products Development that just accelerating.Most of semiconductor industries comprise the operation at (1) array (array), (2) colour filter (color filter) and (3) liquid crystal, and in these operations as developing solution, washings, and etching reagent etc. and use a large amount of organic solvents, its result generates a large amount of waste water.The characteristics of this waste water are except dimethyl sulfoxide (DMSO) (DMSO) or Virahol organic solvents such as (IPA), also contain considerable tetramethyl ammonium hydroxide (TMAH).
Because TMAH is difficult to biological decomposition take place and have strong toxic material (toxicityLC50=460PPM), thereby present situation is the biochemical oxygen demand (BOD) of the waste water processing station in semiconductor fabrication factory (biological oxygen demand at present; BOD) drastic change takes place.Thereby, for environment protection, how from industrial wastewater, to remove TMAH, become important topic.
At present the known method that TMAH in the waste water is handled has: concentrating behind the TMAH again to water reuse ion exchange resin column, UF (ultrafiltration) or RO methods such as (reverse osmosiss), depleted concentrates retirement method; Perhaps, the first supercritical processing method that spissated TMAH burning is removed etc.Yet these purifying methods in the past not only will be finished through very complicated step, but also exist by concentrating or the significant deficiency such as the secondary pollution problem occur of burning.Thereby day by day enhanced is to requirement on environmental protection for correspondence, and people recognize not only economy but also the necessity of TMAH bioremediation efficiently deeply.
MBR operation (with reference to Fig. 1) in wastewater treatment is removed the solid substance that swims fully owing to can utilize the stalling characteristic of film, therefore can under the situation that not influenced by sludge settling, carry out stabilizing treatment, and it is different with the mode that possesses settling tank of Fig. 2, need not possess settling tank, and can pile up the microorganism of processing of high density at short notice, therefore can carry out a large amount of water treatments with little space and promptly can make numerical value treatment time (HRT) maximization, and then can reduce the volume of operation, realize the densification of operation.In addition, in the advantage of MBR most important one be to carry out steady running.Because this method has and can maximize to detention time of mud (SRT), thereby significantly reduced to improve the various advantages of processing efficiency the biological treatment time, therefore get most of the attention as the technology of the following problem that exists in the active sludge operation that can remedy in the past, wherein, the problem of the described operation of active sludge in the past is meant, has the low problem of settleability worsens, contains the inflow of the waste water of high density toxicant, the impact load that brings for the variation of treatment condition etc. shows vulnerability problem or processing efficiency etc.
The barrier film that uses at environmental area may be defined as the barrier (barrier) that can separate two phases (phase), can be defined as two between the agllutination crystalline substance the interface or only selectively some material could pass through semi-permeable membranes (semipermeable) barrier of its structure.
The starting material of at present commercially available accurate barrier film are divided into macromolecular and inorganic system, wherein the starting material as inorganic system use pottery (mainly being Alpha-alumina), glass, carbon etc., and adopt split-phase method (glass material) and sintering process (ceramic membrane) etc. as film manufacturing method.
Wherein, one of advantage of ceramic barrier film is that stability to high temperature or acid, alkali is than polymeric membrane height.Two of advantage is to produce shape and the form ceramic membrane more favourable than polymeric membrane.Three of advantage is owing to the physical strength height, and therefore the fine pores under condition of high voltage is with low uncertainty, and then can normally operation under condition of high voltage.Four of advantage is its practical lives for than longer about 3～5 years of organic plasma membrane, and the inorganic nature starting material are few to the pollution level of microorganism or organic property matrix, in addition, since can be under the temperature condition higher or acid, carry out CIP under the alkaline purification than polymeric membrane, therefore its practical life through keeping reparation can be very long.In addition and since transmitance than organic property film good and safe to toxicant, organic solute, pollution substance etc., therefore in environmental treatment, use manyly than polymeric membrane.
Summary of the invention
The objective of the invention is to solve described problem, a kind of method of wastewater treatment that utilizes above-mentioned bacterial strains and effectively remove the TMAH in the waste water through the MBR operation is provided.
Another object of the present invention provides removes the novel strain with hard-decomposed and toxic TMAH a kind of can effectively the decomposition.
For achieving the above object, the biological effluent treatment method that the invention provides a kind of bacterial strain and utilize described bacterial strain, wherein, described bacterial strain is effectively to decompose the novel strain that contains and have hard-decomposed and toxic TMAH in photoelectronic industry waste water in a large number at normal temperatures.
Bacterial strain of the present invention has carried out isolation identification by following process.
At first, the waste water of the domestic semiconductor factory of Korea S as sample collection, is inoculated into sample on the nutritious substratum then, the bacterial strain that exists in the property ground breeding waste water and select 12 kinds of bacterial strains again is at last with each bacterial strain called after IBN-H01～H12.Then, in the substratum that is containing TMAH under 30 ℃, above-mentioned bacterial strains cultivated and make it growth, and then isolate 5 kinds of bacterial strains that TMAH had patience and TMAH decomposability excellence.
3 kinds of bacterial strains to wherein TMAH decomposability excellence, utilize the determining etc. of part base sequence of biosystem, MIDI and 16s RNA and identify, by its result as can be known, 2 kinds in above-mentioned 3 kinds of bacterial strains are respectively and the IBN-H1 and the identical bacterial strain of IBN-H7 that carried out describing in detail in the special 2002-0009060 of Korea S's publication.Remaining a kind to be confirmed as be the novel strain that belongs to acinetobacter (Acinetobacter sp.), thereby called after acinetobacter IBN-H6, and on May 24th, 2005 to having handled preservation with trust KCTC 10809BP as the Korea S typical case culture collection center (KCTC) of the international associated agency of bacterial strain.
Understood fully in addition and can grow to and make the OD value become about 0.2 degree as must mineralogical composition only containing in the minimal medium of TMAH above-mentioned separated novel strain with carbon source.In addition, can there be very big-difference ground growth down at 30 ℃, and can growth well under neutrality and even solutions of weak acidity.
Not only TMAH is had patience according to bacterial strain provided by the invention, and it can be grown as sole carbon source, therefore can utilize above-mentioned bacterial strains that the waste water that contains TMAH is carried out a biological disposal upon.At this moment, can use above-mentioned bacterial strains separately, also can use with the state that mixes 2～4 kinds of cultivations with 3 kinds of bacterial strain IBN-H1, IBN-H4 in Korea S publication 2002-0009060 number, carrying out describing in detail and IBN-H7 at the TMAH capacity of decomposition.
In the TMAH treatment system, the mark degree of the microorganism that must in existing waste water processing station, exist
Figure A20051011877500051
Under high large amount of organic and the toxicity as TMAH and organic waste coexistence and the absolute exhausted state of carbon source TMAH is decomposed, but owing in fact be difficult to realize, separate and independent processing with waste water after therefore in the TMAH treatment process, preferably the microorganism IBN-H6 of the present invention of TMAH capacity of decomposition excellence being cultivated artificially separately or with the mixing microorganisms of IBN-H6 and other microorganisms.
In above-mentioned wastewater treatment operation any one operation applicable to known biologic treatment process in the past, but because above-mentioned TMAH processing microbial cell size is littler than the microorganism of general waste water processing station, therefore consider the characteristic of microorganism and the adaptibility to response of processing efficiency maximization and impact load etc., preferably use the MBR processing mode.If with the strong TMAH of the aforesaid bio-toxicity of MBR mode individual curing, then have other water treatment operations such as not only not influencing denitrogenation, dephosphorization and also do not need to carry out to handle for 2 times, the mud generation is few, the advantages such as influence that do not flowed into the TMAH concentration in the water.But also owing to possess space efficiency, therefore as shown in table 1, comparing with equipment in the past only needs 1/18～1/35 space, and can realize thus taking up an area of and minimize, and these external equipment maintenance costs are also very cheap.
[table 1]
Distinguish TMAH separates aftertreatment separately Combination treatment
The technology of the present invention General device (S electronics society) General device (general aeration tank)
Space efficiency (m 3/kg TMAH) 0.11 2.05 3.79
(relatively) (1.0 benchmark) 18.6 34.5
At above-mentioned MBR mode septation (membrane) ceramic membrane preferably.Different with the polymeric membrane that generally uses, ceramic membrane has chemical stability and physical robustness and can import reverse impulse (backpulse), therefore can overcome the running problem that the characteristic by waste water and microorganism causes.
In addition, in order to be applicable to the general biological operation beyond the above-mentioned MBR mode, carry out easily bacterial strain recycle and treating water in the bacterial strain continuous processing of removing in, preferably above-mentioned bacterial strains is fixed on the regulation carrier and utilizes.At this moment, can use alginate (alginate), polyurethane foam common microorganisms such as (urethane foam) fixedly to use material as carrier.
Description of drawings
Fig. 1 is the synoptic diagram of expression MBR wastewater treatment equipment of the present invention.
Fig. 2 is the synoptic diagram that the common settling tank wastewater treatment equipment of conventional art is adopted in expression.
Fig. 3 is the chart of expression each bacterial strain of the present invention for the existence density of different TMAH concentration.
Fig. 4 is the system diagram of expression IBN-H6 of the present invention.
Fig. 5 is the graphic representation based on the growth curve of culture temperature of expression IBN-H6 of the present invention.
Fig. 6 is the graphic representation based on the growth curve of cultivating pH of expression IBN-H6 of the present invention.
Fig. 7 is the graphic representation that is illustrated in the bacterial strain concentration when carrying out MBR wastewater treatment of the present invention.
Embodiment
To be described in detail embodiments of the invention below.These embodiment only are used to specify the present invention, and the personnel of the general knowledge in grasp the technology of the present invention field know that certainly scope of the present invention can not limited by these embodiment.
Embodiment 1: TMAH is possessed the separation of the bacterial strain of patience
(1) breeding of the bacterial strain that in semiconductor factory's waste water, exists
In the biological organic process operation and denitrogenation processing operation of domestic each semiconductor fabrication factory of Korea S, wastewater sample such as former water, water aeration, returned sluge have been prepared to flow into.
Dilute after the said sample in order, it is applied on the abundant trophicity Agar Plating (beef extract 0.3% (DIFCO), peptone 0.5% (DIFCO), agar 1.5% (DIFCO)) of carbon source, cultivated 18 hours down at 30 ℃, and select 12 kinds of bacterial strains, with they difference called after IBN-H01～IBN-H12.
(2) TMAH had the separation of the bacterial strain of patience
Carried out the judgement that whether TMAH had patience for the bacterial strain that obtains in the breeding stage.
In the abundant substratum (beef extract 0.3% (DIFCO), peptone 0.5% (DIFCO)) of carbon source, make the concentration of TMAH be respectively 0.25%, 0.5%, 1.0%, 1.5%, 2.0% and smear the bacterial strain of above-mentioned breeding after, with the speed of 250rpm 30 ℃ down concussion cultivated 24 hours.
From wherein selecting 5 substratum (IBN-H01, IBN-H04, IBN-H05, IBN-H06 and IBN-H08) that with the naked eye to confirm thalli growth, and the final nutrient solution that will select in order dilution method be applied on the above-mentioned trophicity Agar Plating, and then (colony forming unit CFU) has represented all bacterial counts (with reference to Fig. 3) to be used in the colony-forming unit that forms in every 1ml nutrient solution.
According to Fig. 3 as can be known, though these 5 kinds of bacterial strains of IBN-H01, IBN-H04, IBN-H05, IBN-H06 and IBN-H08 all exist 1.7% to demonstrate some survival rate reductions when above at TMAH, but in addition, as strong patience, till 1.5%, shown that height is to the survival rate more than 108 to TMAH.Especially under the situation of IBN-H06, till the concentration range of low TMAH concentration to 2.0%, demonstrated even and good survival rate.
Embodiment 2: carry out the investigation of TMAH rate of decomposition for the bacterial strain that possesses TMAH patience that is selected
TMAH rate of decomposition for the TMAH patience bacterial strain that is selected in embodiment 1 is investigated.
Containing the minimal medium ((NH of mineralogical composition essential concerning strain growth 4) 2SO 43g/L, trace element (H 3BO 30.3g, CoSO 47H 2O 0.2g, ZnSO 4H 2O 0.1g, MnCl 2H 2O0.03g, NaMoO 4H 2O 0.03g, NiCl 2H 2O 0.02g, CuSO 4H 2O 0.01g, FeSO 4H 2O20g, CaCl 2H 2O 10g/L) 2ml/L, MgSO 47H 2O (40%) 2ml/L, 1M phosphate buffer soln (pH7)) in, add 1%TMAH as carbon source, and shake cultivation 72 hours down at 30 ℃.
The result who measures according to the BOD that utilizes triple experiments (triple test) to final nutrient solution has shown the BOD clearance (with reference to table 2) more than 90% as can be known in IBN-H04, IBN-H05 and IBN-H06 bacterial strain.
[table 2]
TAG TMAH concentration before the dilution 10 -2Dilution back TMAH concentration Prediction BOD BOD Clearance
Sample size (ml) DO 4 DO 5 BOD 5
IBN-H01 1％ 100ppm 180→ 100～5 10 9.15 6.85 69 61.7％
IBN-H04 1％ 100ppm 180→ 100～5 10 9.1 8.73 11.1 93.8％
IBN-H05 1％ 100ppm 180→ 100～5 10 9.2 8.94 7.8 95.7％
IBN-H06 1％ 100ppm 180→ 100～5 10 9.15 8.98 5.1 97.2％
IBN-H08 1％ 100ppm 180→ 100～5 10 9.15 6.3 85.5 52.5％
1% substratum 1％ 100ppm 180→ 200～ 100 5 9.2 6.1 186
2.38％STD 2.38％ 238ppm 430→ 450～ 350 2 9.25 6.3 442
The evaluation of the bacterial strain of embodiment 3:TMAH capacity of decomposition excellence
For 3 kinds of bacterial strain IBN-H04, the IBN-H05 and the IBN-H06 that in embodiment 1, pick out and in embodiment 2, confirm TMAH capacity of decomposition excellence, utilize biological test, fatty acid analysis, MIDI and 16S rRNA part base sequence etc., carried out the evaluation of bacterial strain.
According to qualification result as can be known, bacterial strain IBN-H04 with IBN-H05 is respectively and IBN-H1 and the identical bacterial strain of IBN-H7 identified in the special 2002-0009060 of Korea S's publication, therefore omission and only is described in detail the qualification result of IBN-H06 the explanation of their data below.
At first, according to the result that morphology and the biochemical characteristic of bacterial strain IBN-H06 of the present invention are investigated as can be known, it possesses catalase activity, and has demonstrated negative (with reference to table 3) in the gramstaining of observing when the Bacteria Identification reaction.
[table 3]
Mobility Gramstaining Catalase The formation of spore Other
Do not have Negative Positive Do not have Good gas
According to the result that the homology of the 16S rRNA part base sequence (sequence number 1) of above-mentioned bacterial strains is analyzed as can be known, its demonstrated and acinetobacter between 94～97% homology (with reference to table 4), therefore be defined as acinetobacter.
[table 4]
Bacterial strain Homology (%) Inconsistent base sequence number/all base sequence numbers
Acinetobacter seohaensis SW100 97 7/349
Acinetobacter sp.DSM 590 97 8/349
Acinetobacter sp，IBN-H7 97 8/349
Acinetobacter ca/coaceticus DSM 30009 96 12/349
Acinetobacter baumannii DSM 30007 96 13/349
Acinetobacter radicresistens DSM 6976 96 13/347
Acinetobacter sp.Z93448 95 14/349
Acinetobacter haemolyticus DSM6962 95 15/349
Acinetobacter johnsonii ATCC 17909T 95 17/349
Acinetobacter twoffii DSM 2403 95 16/347
Acinetobacter junii ATCC 17908T 94 19/349
Because at present there are 8 kinds in acinetobacter, and not have similarity be kind 98% or more, and therefore judgement is new kind.As mentioned above, can confirm that bacterial strain IBN-06 of the present invention is the novel strain of not reported, thereby called after acinetobacter IBN-H6 (Acinetobacter sp.IBN-H6), and on May 24th, 2005 to having handled trust (following IBN-H06 is designated as IBN-H6) with trust KCTC 10809BP as the Korea S typical case culture collection center (KCTC) of the international associated agency of bacterial strain.In addition, for representing that this bacterial strain is that to decompose bacterial strain with in the past TMAH be that acinetobacter IBN-H7 and bacillus cereus belong to the different novel strain of IBN-H4, uses system diagram (phylogenic tree) (with reference to table 4) to represent that mentioned microorganism and other have the bacterial strain of the relation of having chance with here.
Embodiment 4: the TMAH usability of the bacterial strain of selected evaluation
To the bacterial strain IBN-H6 of the present invention of above-mentioned evaluation whether can with TMAH as sole carbon source utilization confirm.
After the TMAH as the additional interpolation 0.5% of carbon source in the minimal medium that contains mineralogical composition essential for strain growth (with reference to embodiment 2), inoculate described bacterial strain, and shake cultivation 72 hours down at 30 ℃.Dilute described nutrient solution in order, produce plate culture medium and smear with composition identical with the nutrient solution composition.
Observe bacterial strain of the present invention and can on each plate culture medium, generate bacterium colony, thereby can confirm that bacterial strain of the present invention can be with TMAH as sole carbon source and energy source utilization and grow.
For comparing,, confirmed its usability to TMAH for having the bacterial strain of having chance with and concerning with bacterial strain of the present invention.
Substitute bacterial strain of the present invention, cultivate acinetobacter lwoffii, acinetobacter calcoaceticus or acinetobacter calcoaceticus gene kind (Acinetobacter genospecies) respectively, whether TMAH can be investigated as utilization of carbon source these.
Its result demonstrates, and all bacterial strains that have the relation of having chance with bacterial strain of the present invention all do not form bacterium colony, and hence one can see that, and these bacterial strains can not be with TMAH as utilization of carbon source.
Can confirm again according to this result, though bacterial strain IBN-H6 of the present invention belongs to acinetobacter,, it be with in the past bacterial strain at the novel strain that has different qualities aspect the biological chemistry.
Embodiment 5: the growth characteristics of the bacterial strain of evaluation
(1) based on the growth characteristics of culture temperature
For determining the optimum culturing temperature of separated bacterial strain, strain separated is inoculated into has in the substratum 200 μ l of the composition identical and be incorporated in 96-well (well) minitype plate, under 25 ℃ and 30 ℃, cultivated 72 hours with the 250rpm concussion with the substratum that uses among the embodiment 2.Use minitype plate reader (MR5000, Dynatech U.S.A) to measure the OD value (with reference to Fig. 5) of bacterial strain at 600nm.
According to Fig. 5 as can be known, in whole culturing process, the better off that the growth fraction under the situation that temperature is 30 ℃ is 25 ℃, and at each temperature along with the passing of incubation time, thalline number (OD value) almost is consistent, and has demonstrated about value of 0.2,0.15 under 30 ℃ and 25 ℃ respectively.
(2) based on the growth characteristics of pH
For the best of determining separated bacterial strain is cultivated the pH value, strain separated is inoculated into has in the substratum 200 μ l of the composition identical and be incorporated in 96-well (well) minitype plate, under the condition of pH5, pH7 and pH9, cultivated 72 hours with the 250rpm concussion in 30 ℃ with the substratum that uses among the embodiment 3.Use minitype plate reader (MR5000, Dynatech U.S.A) to measure the OD value (with reference to Fig. 6) of bacterial strain at 600nm.
According to Fig. 6 as can be known, the OD value almost is 0 under the condition of pH9, but then shows about 0.2 OD in pH5～7 scopes, thereby can grow well under neutrality and even solutions of weak acidity as can be known.
Embodiment 6: the MBR mode treatment process of utilizing the TMAH of bacterial strain of the present invention
Owing to confirmed in the above-described embodiments bacterial strain of the present invention can with TMAH as sole carbon source utilization grow, therefore handle the waste water that contains TMAH in the MBR mode, confirmed its efficient.As a rule, if use single culture then stability problem may occur, therefore used the hybrid bacterial strain of bacterial strain IBN-H6 of the present invention and other bacterial strains in the present embodiment.
(1) in operation, using the thalline high-density culture of carrying out
In order in the water treatment of the waste water that contains TMAH, to use, respectively with high-density culture 3 kinds of bacterial strain IBN-H1,4,7 of bacterial strain IBN-H6 of the present invention and explanation in open 2002-0009060 number of Korean Patent.
Comprising high density production substratum (glucose 20g/L, yeast extract 15g/L, MgSO 47H 2O 2g/L, KH 2PO 45g/L, (NH 4) 2SO 44g/L, pH 7.0 (HCl)) in the culture tank of 500L capacity of 350L, inoculate bacterial strain of the present invention respectively, 30 ℃ of following stir culture, append the normal carbon source of glucose 10g/L in the inoculation beginning after 12 hours simultaneously, with aforesaid method cultivation 24～48 hours.
Will be through pure the cultivation and OD reaches the bacterial strain IBN-H6 of the present invention that is trained high density more than 40 and IBN-H1,4,7 strain cultured solution 1200L and is seeded among 12 tons of the TMAH waste water.
(2) being suitable for to TMAH wastewater treatment operation of bacterial strain of the present invention
The operating condition of fermenter is that the TMAH concentration of keeping the raw wastewater of 25～35 ℃ of culture temperature, inflow air capacity 0.5vvm, inflow is 3～5g/L, and utilizes sulfuric acid that pH is adjusted to 6.5～7.5.For the stabilization of thalline, with 24 hours served as at interval 1/10 of nutrient solution is concentrated, the inflow of 11 tons TMAH waste water implemented 3 days, and as nutrition source, with TMAH 5g/L, (NH 4) 2SO 43g/L, yeast extract 0.3g/L, MgSO 47H 2O 0.3g/L is with this concentration additional input.The capacity that carries out the continuous operation operation after the thalline stabilization in reactor is 14～16 tons, and pH is 6.5～7.5, treatment temp is 25～35 ℃.Beginning in the 4th day behind the sowing bacterial strain, inflow and outflow contains the waste water of the TMAH of 3～5g/L concentration, simultaneously the treating water of discharging is carried out membrane sepn, thereby thalline is recycled to fermenter again, handles continuously thereby carried out MBR.
With the concentration of microorganism as time passes and in the reactor that changes after the continuous operation,, be illustrated among Fig. 7 by measuring dry biomass and the bacteria total amount of living.As shown in Figure 7, cell concentration increases till the 5th day at continuous operation always, and carries out the taking-up of mud after the 3000ppm degree with 5～20kg/L level in the sensitization of MLSS concentration.Average mathematics retention time with 7～8 hours is kept 50m with wastewater treatment capacity 3/ day.The pH of the raw wastewater that flows into is 12～13, and makes it to flow into after in the pH regulator pond pH being adjusted to 9.5～10.5 in advance, and the pH of effusive waste water is 7.0.The processing efficiency of TMAH begins to begin after 3 days processed more than 90% from continuous operation, and the MLSS in the fermenter keeps 3000～15000ppm after the continuous operation 15 days, and the intermembranous pressure difference (TMP) in this external membrane sepn operation is about 0.7 crust (bar).More detailed operating condition when table 5 has been represented continuous operation.
[table 5]
The fermenter condition
Temperature (℃) 25～35 Air flow (aeration) 0.5vvm
Retention time (hr) 8 The operation capacity 14～16 tons
Intermembranous pressure (bar) 0.5～1.5 MLSS(ppm) 3000～15000
Total cfu (/mL) 1.0×10 9～5.0×10 9
The wastewater treatment condition
Flow into Flow out Relatively
Processing capacity (m 3/ day) 50 50 Mud: 0.3～1
TMAH concentration 3～5g/L 0.03～0.04g/L Clearance: more than 90%
Working hour 24h/ days 24h/ days More than 3 months
pH
12～13 7.0 PH regulator pond: 9.5～10.5
As shown in Figure 5, bacterial strain concentration in the fermenter in the wastewater treatment not too be subjected to TMAH inflow influence and can stably keep, and when continuous operation is more than 3 months, also do not have big variation and stable maintenance, and its processing rate also rises average 90% with respect to the TMAH raw wastewater.
(effect of invention)
According to the present invention, can provide can decompose in semi-conductor manufacturing process the novel strain that mainly uses when silicon face carried out etching and utilize this bacterial strain with hard-decomposed and toxic tetramethyl ammonium hydroxide contain the TMAH wastewater treatment method.
According to method of wastewater treatment of the present invention, can provide microorganism that employing can use hard-decomposed TMAH as sole carbon source and the MBR mode of keeping high concentration microorganism concentration and with the maximized environmentally friendly water treatment method of the processing efficiency of TMAH.
In addition, cost of equipment and standing charges are few, and space efficiency is very high, and because the strong TMAH of individual curing bio-toxicity, thereby do not influence other water treatment operations.In addition, owing to have the influence that is not too flowed into the interior TMAH concentration of water and do not need advantages such as 2 processing and mud generation lack, therefore be expected to as environmentally friendly method of wastewater treatment on-the-spot widely-used in industry.
[sequence table]
＜110〉In Bionet Inc.
＜120〉method of wastewater treatment that can decompose the novel strain of tetramethyl ammonium hydroxide and utilize described bacterial strain
<160>1
<170>KopatentIn 1.71
<210>1
<211>349
<212>DNA
＜213〉acinetobacter (Acinetobacter sp.)
<400>1
gaccttgcgc taatagatga gcctaagtcg gattagctag ttggtggggt aaaggcctac 60
caaggcgacg atctgtagcg ggtctgagag gatgatccgc cacactggga ctgagacacg 120
gcccagactc ctacgggagg cagcagtggg gaatattgga caatgggggg aaccctgatc 180
cagccatgcc gcgtgtgtga agaaggcctt ttggttgtaa agcactttaa gtggggagga 240
ggctctcttg gttaatacct aagaagagtg gacgttaccc acagaataag caccggctaa 300
ctctgtgcca gcagccgcgg taatacagag ggtgcgagcg ttaatcgga 349

Claims (6)

1. bioremediation that contains the waste water of TMAH, it is the wastewater treatment method that contains TMAH, wherein adopts the MBR mode.
2. the bioremediation that contains the waste water of TMAH as claimed in claim 1, wherein,
Described method of wastewater treatment utilizes acinetobacter IBN-H6 (Acinetobacter sp.IBN-H6; KCTC 10809BP) bacterial strain.
3. the bioremediation that contains the waste water of TMAH as claimed in claim 2, wherein,
Kluvermyces delphenisis IBN-H1 (KCTC 0834BP), bacillus cereus IBN-H4 (Bacillus cereus IBN-H4 have also been appended; KCTC 0835BP), acinetobacter IBN-H7 (Acinetobacter sp.IBN-H7; KCTC 0836BP) an above bacterial strain in the bacterial strain.
4. the bioremediation of waste water as claimed in claim 1, wherein,
The film that uses in the treatment process of described MBR mode is a ceramic membrane.
5. as the bioremediation of claim 2 or 3 described waste water, wherein,
Described bacterial strain is carried out immobilization.
6. a bacterial strain is acinetobacter IBN-H6 (Acinetobacter sp.IBN-H6; KCTC 10809BP) bacterial strain, it has patience to tetramethyl ammonium hydroxide, and can be with described chemical substance as carbon source for growth.
CNB200510118775XA 2005-07-22 2005-10-31 Novel strain capable of decomposing tetramethyl ammonium hydroxide and method for treating waste water using the strain Expired - Fee Related CN100417721C (en)

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