KR20140114564A - Complex strain for the waste water treatment and nitrogen treatment process using the same - Google Patents
Complex strain for the waste water treatment and nitrogen treatment process using the same Download PDFInfo
- Publication number
- KR20140114564A KR20140114564A KR20130028943A KR20130028943A KR20140114564A KR 20140114564 A KR20140114564 A KR 20140114564A KR 20130028943 A KR20130028943 A KR 20130028943A KR 20130028943 A KR20130028943 A KR 20130028943A KR 20140114564 A KR20140114564 A KR 20140114564A
- Authority
- KR
- South Korea
- Prior art keywords
- nitrobacter
- nitrogen
- present
- tank
- strain
- Prior art date
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/30—Wastewater or sewage treatment systems using renewable energies
- Y02W10/33—Wastewater or sewage treatment systems using renewable energies using wind energy
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
Description
More particularly, the present invention relates to a new nitrophenyl ether which is excellent in the decomposition ability of nitrate in organic wastewater and has a high concentration of nitrogen removal ability which is high in growth rate, and a representative organic waste, We have developed a biological system that can effectively treat digestion effluent, which has been a major weakness in the application of bioreactor process, as well as efficient production of bio-energy and high quality livestock and existing anaerobic digestion process. .
Most of the livestock manure is registered as a compost and liquid fertilizer in the livestock waste wastewater in Patent Registration No. 10-0921194. However, environmental damages are serious due to the manure of the manure, unsteady discharge of the liquid manure, and odor when spraying the manure, Losing confidence and spreading a lot of liquid on less land, making soil and water pollution more serious. In addition, farmers who have difficulty in making fertilizer resources install clean treatment facilities, but there are many technical and economic difficulties in handling livestock manure at high concentration than general sewage or factory wastewater.
On the other hand, modern society is supported by fossil energy, nuclear energy, hydro energy, wind energy, etc. However, in most regions and countries, fossil energy accounts for more than 80%. However, the fossil energy represented by petroleum and coal is not sustainable due to limited availability, and it causes not only international disputes due to uneven distribution in regions but also greenhouse gas such as carbon dioxide, carbon monoxide and nitric oxide, El Niño and so on.
In the case of developed countries, biogas generated by anaerobic digestion is recognized as clean renewable energy, and active research is under way. On the other hand, Korea, as in Patent Registration No. 10-1207532, The development of biogas production and utilization technology using biogas has not yet reached the basic stage. .
Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in order to solve the problems of the prior art as described above, and it is an object of the present invention to separate nitrifying microorganisms capable of growing under high concentration organic wastewater conditions, It is an object of the present invention to provide a method for use as an activity promoting agent for improving not only physico-chemical properties but also biological properties, or for use as a microbial agent capable of promoting high-efficiency purification and production of biogas.
In addition, from the conventional purification of the water quality, purification of highly purified wastewater can be practically reused, reducing the amount of surplus sludge that is problematic in the conventional purification treatment, and facilitating the growth of microorganisms, which are the main cause of water quality. To achieve a purification treatment facility that is economical maintenance cost without necessity.
Technical Challenge
The object of the present invention is to provide Nitrobacter sp
An object of the present invention is to provide a highly efficient nitrification tank in which a strain of Nitrobacter sp. H2S1 is added, a highly efficient nitrification tank using the same, and a livestock wastewater treatment system.
In addition, the present invention provides a method for improving an animal manure processing system using the complex microorganism preparation.
Technical Solution
In order to achieve the above object,
The present invention provides a novel Nitrobacter sp H2S1 capable of removing organics, particularly nitrogen, very quickly and efficiently than conventional strains.
The present invention has shown that the above-mentioned Nitrobacter sp H2S1 significantly reduced sludge and nitrogen in livestock wastewater. Therefore, the microbial preparation according to the present invention can be widely used for purifying livestock wastewater.
An anaerobic tank for denitrifying the nitrate treated in the aerobic tank;
A nitrification tank for nitrifying ammonia and nitrite not decomposed in the anaerobic tank;
A sterilizing / disinfecting tank for introducing the treated water settled in the anaerobic tank and removing micro-molecules from the electrolyzer to sterilize;
And a system for supplying oxygen and energy for microbial growth of each nitrification tank and nitrite tank by reusing the high concentration of oxygen and surplus energy generated in the sterilization / disinfection tank.
The object of the present invention is to provide Nitrobacter sp H2S1 for removing sludge generated from wastewater generated from livestock manure and high concentration of nitrogen.
An object of the present invention is to provide a highly efficient nitrification tank in which Nitrobacter sp. H2S1 is added, a high efficiency nitrification tank and a livestock wastewater treatment system using the same.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 schematically shows a purification apparatus used in a method for purifying livestock wastewater according to the present invention. FIG.
FIG. 2 is a phylogenetic analysis of a novel Nitrobacter sp. H2S1 strain of the present invention using a 16S rDNA gene sequence.
The present invention provides a new strain of Nitrobacter sp. Specifically, the present invention provides a Nitrobacter sp. Strain isolated from wastewater having a function of purifying livestock wastewater.
It is preferable that the strain is a Nitrobacter sp. Nitrobacter sp. H2S1 strain of the present invention.
Quot; Nitrobacter sp. ≪ / RTI > H2S1 " of the present invention.
The present invention provides a method for separating Nitrobacter sp. H2S1 strain from livestock wastewater.
In the present invention, prior to explanation of the organic matter removal and nitrogen removal of the livestock wastewater, the nitrogen removal apparatus used in the nitrogen removal method of the present invention will be described.
As shown in FIG. 1, an apparatus used for purifying livestock wastewater according to the present invention includes a desalination tank (T-101), a flow storage tank (T-102), a first nitrification tank (T-103) (T-109), a first nitrification tank (T-105), a second anoxic tank (T-106), a second nitrification tank (T- , A disinfection / disinfection tank (T-110), and a settling / discharging tank (T-111).
The storage tank (T-101) is a space for storing the manure from the housing, and the manure is supplied to the flow storage tank (T-102) only by the solid-liquid separator or the sheave screen.
The first nitrification tank (T-103) oxidizes ammonia (NH3) of the desorbed liquid introduced in a flow rate storage tank (T-102) into a nitrite or a nitrate by the action of nitrifying bacteria or nitrifying bacteria, (T-110), oxygen and heat generated during electrolysis are supplied, and the internal temperature is maintained at 34 to 40 DEG C, so that nitrifying bacteria are suppressed and nitrite bacteria growth is dominant.
The first anoxic tank T-104 is operated to remove nitrite (NO2) or nitrate (NO3) of the desorbing liquid introduced from the first nitrification tank (T-103) by nitrifying microorganisms using nitrite or nitrate as an electron acceptor (N2).
The first nitrification tank (T-105) oxidizes ammonia (NH3) of the digested liquid that has been internally transported in the second nitrification tank and the desorption liquid introduced from the first anoxic tank (T-104) 2 anoxic tank (T-106). In particular, Nitrobacter sp (Nitrobacter sp) is added in a quantitative manner to remove nitrogen from high concentration livestock wastewater, thereby enhancing nitrification efficiency.
The second anaerobic tank T-106 converts the nitrate of the desorbing liquid introduced from the second nitrifying tank (T-105) into nitrogen gas by denitrifying microorganisms using nitrate as an electron acceptor to denitrify do.
The second nitrification tank (T-107) serves to oxidize ammonia (NH3) not removed in the second anaerobic tank (T-106) to nitrite.
The third anoxic tank (T-108) serves to convert nitrite or nitrate introduced from the second nitrifying tank (T-107) into nitrogen gas.
The aeration tank (T-109) serves to remove surplus nitrogen and phosphorus
The sterilization and disinfection unit (T-110) is a specially developed electrolytic unit, which removes chromaticity, disinfection and surplus nitrogen.
Finally, the sedimentation tank (T-111) serves to separate the sludge from the desorbing liquid introduced from the disinfection / disinfection tank, and a part of the separated sludge is returned to the first anoxic tank (T-104).
In the present invention, all the groups are filled with microbial filter media, thereby increasing the efficiency of anaerobic / aerobic digestion. In order to enhance the sludge reduction effect, the microbial filter media are specially developed in four directions.
Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood, however, that the following examples are presented for the purpose of illustrating the invention and are not to be construed as limiting the scope of the invention.
[Example 1] Isolation of a strain of Nitroobacter from livestock wastewater
In order to isolate a strain having nitrification ability from livestock wastewater and having an excellent organic decomposing ability, a sample was prepared from livestock manure by adding 5 g of Bacto peptone, 1 g of Bacto yeast extract, 0.1 g of Fe (III) citrate, 5.9 g of MgCl 2 (dried) (NH4) NO3, 8 mg of Na2HPO4, 33 g of NaCl and 15 g of agar were added to 1 liter of distilled water, and the mixture was stirred at room temperature for 2 hours. And sterilized at high temperature. And cultivated at 30 ° C for 24 hours. A total of 20 strains were selected.
The selected strains were selected from 5 strains that can utilize a wide spectrum of carbon sources through various carbon source experiments. The temperature, pH, and NaCl concentration required for their growth were measured. ~ 42 ℃ temperature, pH 6 ~ 10 and 1 ~ 2% NaCl concentration, indicating that there is a possibility of application in a wide range of environments where these conditions are met.
As a result of testing the decomposition ability of high concentration organic matter in the 5 selected strains, the strain H2S1 having excellent organic decomposition ability was finally selected and the activity of the strain was confirmed through subculture. For the next experiment,
(Glycerol stock).
[Example 2] Identification of isolated H2S1 strain of Nitrobacter
The nucleotide sequence of the 16S rDNA of the strain H2S1 having excellent selectivity for organic matter resolution was analyzed. Genomic DNA was obtained from the strain using DNeasy Tissue Kit (Qiagen, Valencia, Calif.) For sequencing of ribosomal 16S rDNA, a method for identification of molecular biological microorganisms, and PCR was performed using universal primers 9F and 1512R Respectively. The PCR products were purified using QIAquick PCR purification kit (Qiagen, Valencia, Calif., USA) and reacted using ABI PRISM BigDye Terminator cycle sequencing ready reaction kit (Applied Biosystems, Foster, Calif., USA) After that, the base sequence was analyzed using ABI Prism 3700 DNA analyzer. The results of the analysis are shown in
The 16S rDNA sequence (SEQ ID NO: 1) of the H2S1 strain obtained through the above-described sequencing method was searched by BLAST (Basic Local Alignment Search Tool). As a result, the H2S1 strain was determined to belong to Nitrobacter sp. (98.0%) with Nitrobacter hmbrgensis X14T, 98.0% with Nitrobacter vulgaris DSM 10236T, 97.85% with Nitrobacter alkalicus AN1T and 97.75% with Nitrobacter hmbrgensis X14T (see Fig. 2).
[Example 3] Physiological and biochemical characteristics of isolated H2S1 strain of Nitrobacter
The H2S1 strain did not form aerobic, gram-negative, spores, and the bacterial form was rod-like (0.8-1.0 x 2.0-4.0 ㎛) with peritrichous flagella.
The Gram stain and biochemical characteristics of the Nitrobacter sp. H2S1 strain of the present invention were positive in the oxydase test and the catalase test as shown in Table 1 below. Using the API 20NE / 50CH / ZYM kit (bioMerieux), various carbon sources could be used as a substrate (see Table 1).
As a result of analyzing the morphological, biochemical and molecular biological classification characteristics of the H2S1, the strain was identified as a new species belonging to the genus Nitrobacter sp. And was identified as a "Nitrobacter sp. H2S1" strain Respectively.
[Example 4]
Nitrogen removal and purification treatment of the desalination liquid was carried out for 3 months at the Hanil Farm, Chungnam Province, using the microorganisms and the purification apparatus used in the nitrogen removal method according to an embodiment of the present invention as shown in FIG.
The water-based mechanism of the high-efficiency decomposition tank is characterized by the simultaneous reaction of adsorption coagulation and sedimentation. It is used as a specially developed microbial media having excellent adsorption ability by maximizing the contact area in water and maximizing the specific surface area of particles. Oxidation and anaerobic digestion are applied at the same time, eliminating nitrogen and phosphorus (Table 2).
(Unit: mg / l)
(Unit: mg / l)
Figure 2. Results of semi-plant water quality analysis in Gudeok-myeon, Yeongyang-gun, Chungnam (TN, TP)
Due to the high-efficiency decomposition tank, organic substances are decomposed to reduce the volume of sediments, reduce the number of sediments, reduce odor, and inhibit the release of water into the aquatic environment through adsorption of heavy metals and pollutants. It can play a role.
In particular, there is a conventional method of using ultraviolet rays and chemicals in the process of disinfection, but the facility cost is excessive, and the cost is low, There was a difficulty to manage. In this technology, the modified ozone sterilizer is optimally used for sterilizing and removing chromaticity, and it is possible to reduce the pollution by not using chemicals.
The wastewater thus treated is purified with the best quality of water and can be cleaned with high efficiency without installing a separate secondary treatment facility, making it possible to treat more easily the inflow water and the inflow amount compared with the conventional treatment method, economical and the best quality water can be recycled . Meanwhile, in the case of high concentration livestock wastewater, the intermediate water settled in the conventional liquid storage tank is applied to the high-efficiency purification treatment of the present invention, Decolorization sterilization and deodorization can be effectively applied. The above-described high-efficiency livestock wastewater purification process according to the present invention is not limited to the above-described embodiments, and can be carried out by a person skilled in the art without departing from the gist of the present invention, Anyone can say that there is a technical spirit to the extent that various changes can be made.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20130028943A KR20140114564A (en) | 2013-03-19 | 2013-03-19 | Complex strain for the waste water treatment and nitrogen treatment process using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20130028943A KR20140114564A (en) | 2013-03-19 | 2013-03-19 | Complex strain for the waste water treatment and nitrogen treatment process using the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20140114564A true KR20140114564A (en) | 2014-09-29 |
Family
ID=51758214
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR20130028943A KR20140114564A (en) | 2013-03-19 | 2013-03-19 | Complex strain for the waste water treatment and nitrogen treatment process using the same |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR20140114564A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190067517A (en) | 2017-12-07 | 2019-06-17 | 주식회사 유앤아이미트 | Novel strains of Oceanobacillus species having activity to reduce foul smell from livestock excretions and a composition containing them |
| KR102368580B1 (en) | 2021-10-07 | 2022-02-25 | 박한용 | Novel strains of high-temperature Bacillus genus having malodor reduction effect on high-temperature livestock manure and livestock manure malodor reduction composition comprising the same |
| KR102541401B1 (en) | 2022-11-10 | 2023-06-14 | 우림바이오 주식회사 | Novel psychrophilic psychrobacillus psychrodurans ub-153 strains having malodor reduction effect and use thereof |
-
2013
- 2013-03-19 KR KR20130028943A patent/KR20140114564A/en not_active Application Discontinuation
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190067517A (en) | 2017-12-07 | 2019-06-17 | 주식회사 유앤아이미트 | Novel strains of Oceanobacillus species having activity to reduce foul smell from livestock excretions and a composition containing them |
| KR102368580B1 (en) | 2021-10-07 | 2022-02-25 | 박한용 | Novel strains of high-temperature Bacillus genus having malodor reduction effect on high-temperature livestock manure and livestock manure malodor reduction composition comprising the same |
| KR102380358B1 (en) | 2021-10-07 | 2022-03-30 | 박한용 | Novel strains of high-temperature Bacillus genus having malodor reduction effect on high-temperature livestock manure and livestock manure malodor reduction composition comprising the same |
| KR102541401B1 (en) | 2022-11-10 | 2023-06-14 | 우림바이오 주식회사 | Novel psychrophilic psychrobacillus psychrodurans ub-153 strains having malodor reduction effect and use thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yang et al. | Highly efficient nitrogen removal of a coldness-resistant and low nutrient needed bacterium, Janthinobacterium sp. M-11 | |
| Tang et al. | Performance and mechanism of a novel algal-bacterial symbiosis system based on sequencing batch suspended biofilm reactor treating domestic wastewater | |
| Du et al. | Simultaneous domestic wastewater and nitrate sewage treatment by DEnitrifying AMmonium OXidation (DEAMOX) in sequencing batch reactor | |
| Xing et al. | Identification of the autotrophic denitrifying community in nitrate removal reactors by DNA-stable isotope probing | |
| Al-Dhabi et al. | Removal of nitrogen from wastewater of date processing industries using a Saudi Arabian mesophilic bacterium, Stenotrophomonas maltophilia Al-Dhabi-17 in sequencing batch reactor | |
| Meng et al. | Efficiency and bacterial populations related to pollutant removal in an upflow microaerobic sludge reactor treating manure-free piggery wastewater with low COD/TN ratio | |
| CN110591947B (en) | Heterotrophic ammonia oxidizing bacteria and application thereof | |
| JP2005034783A (en) | Nitrification/denitrification method for removing nh4+ and no3- simultaneously | |
| CN101386823B (en) | Special effect anaerobic denitrifying bacterium and waste water processing method using thereof | |
| Wang et al. | Nitrogen removal intensification of aerobic granular sludge through bioaugmentation with “heterotrophic nitrification-aerobic denitrification” consortium during petroleum wastewater treatment | |
| Wang et al. | Quantitative impact of influent characteristics on nitrogen removal via anammox and denitrification in a landfill bioreactor case | |
| Wang et al. | Nitrogen removal performance and ammonia-and nitrite-oxidizing bacterial community analysis of a novel industrial waste-based biofilter | |
| CN101580332B (en) | Tubificidae-microorganism symbiotic system muddy water degradation batch reactor and application thereof | |
| KR20140114564A (en) | Complex strain for the waste water treatment and nitrogen treatment process using the same | |
| CN104291444B (en) | A kind of contact hydrolysis-algae micro-aeration compound sewage processing system and method | |
| CN111621438B (en) | Wedner mannich bacillus LM-LZ separated from oxidation pond of pig farm and application thereof | |
| US20210380452A1 (en) | Method for treatment and resource utilization of acidic organic wastewater | |
| KR100828566B1 (en) | 4 Pseudomonas fluorescens K4 having excellent ability of denitrification | |
| CN110157637A (en) | Enterobacteria Z1 and klebsiella Z2 composite bacteria agent removal high nitrogen pollutant effluents and application | |
| CN110628670B (en) | Heterotrophic nitrifying bacteria and application thereof | |
| CN110144308B (en) | High-salt-tolerance denitrifying bacterium capable of efficiently degrading nitrate, and preparation and application thereof | |
| JP2006211908A (en) | New microorganism and method for nitration-treating ammonia-containing water by utilizing the same | |
| CN108410754B (en) | High-efficiency JM (JM) bacteria technology for treating high-salt heavy-metal degradation-resistant organic wastewater and resisting bacteria and deodorizing | |
| KR20100048352A (en) | Klebsiella pneumoniae kc-101 for effective of denitrification | |
| JP4670425B2 (en) | Novel degrading bacteria and method for decomposing organic compounds using the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WITN | Withdrawal due to no request for examination |