CN1879731A - A method for rapidly extracting fat soluble component from biological material - Google Patents
A method for rapidly extracting fat soluble component from biological material Download PDFInfo
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- CN1879731A CN1879731A CNA2006100168563A CN200610016856A CN1879731A CN 1879731 A CN1879731 A CN 1879731A CN A2006100168563 A CNA2006100168563 A CN A2006100168563A CN 200610016856 A CN200610016856 A CN 200610016856A CN 1879731 A CN1879731 A CN 1879731A
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Abstract
The invention relates to a method for quickly extracting fatsoluble component from biological material, which uses the biological matter with fatsoluble components of plant, animal or bacterial as raw material, uses non-conductive fatsoluble organic solution as dissolvent; to process the mixture between powder and dissolvent at normal temperature and high-voltage impulse electric field to extract the fatsoluble component, while the electric field strength is 2-60KV/cm and the impulse number is 1-300. Compared with traditional method, the invention can avoid heating, with less time and high yield.
Description
Technical field
The present invention relates to a kind of from biological material the method for rapid extraction liposoluble constituent.
Background technology
The said biological material of the present invention comprises plant, animal or mushroom, and some contains liposoluble constituent in the biological material.The method liposoluble constituent that extracts is a lot of at present, can be divided into traditional extraction process and modern extracting method.
Traditional extraction process has: the heated solvent leach extraction method, and as soxhlet extraction.This method can be extracted liposoluble constituent, but has following shortcoming: 1) extraction time is long; 2) leaching process generally will heat, and consumes the more energy.
Modern extracting method has: supercritical liquid extraction technique, the solvent of this method are liquid carbon dioxide, and it extracts liposoluble constituent yield height.But this method also exists shortcomings such as energy consumption height, extraction time length, apparatus expensive.
Summary of the invention
The objective of the invention is to the above-mentioned shortcoming that exists at existing liposoluble constituent extractive technique, provide a kind of at normal temperatures, extraction rate is fast, extraction ratio is high, efficient energy-saving, application a kind of more widely from biological material the method for rapid extraction liposoluble constituent.
The present invention a kind of from biological material the method for rapid extraction liposoluble constituent, be to be raw material with the plant, animal or the mushroom biological material powder body that contain liposoluble constituent, with nonconducting fat-soluble organic solution as solvent, it is characterized in that it being that mixed solution with described powder material and solvent passes through high voltage pulse electric field processing at normal temperatures, its electric field intensity is that 2～60kV/cm, umber of pulse are 1～300, with the material centrifugalize behind the high voltage pulse electric field processing, get supernatant, solvent is removed in volatilization, promptly obtains solid-state liposoluble constituent.
The biological material that utilizes this method to extract can be the material that plant, animal, mushroom etc. contain liposoluble constituent, for example: Semen sojae atricolor, Semen arachidis hypogaeae, Semen Sesami, bone meal, Fructus Hippophae, Pericarpium Citri tangerinae, Radix Ginseng, Inonqqus obliquus etc.The biological material coarse powder also can be able to be carried out micronizing.
The employed solvent of liposoluble constituent that utilizes this method to extract biological material is nonconducting fat-soluble organic solvent, for example: petroleum ether, ether, acetone, normal hexane etc.
The solid-liquid ratio of used material of this method and solvent solution is very little to the influence of the extraction yield of liposoluble constituent, as long as material can be enough to by solvent institute's submergence and guarantee the abundant stripping of liposoluble constituent in the material, can adjust solid-liquid ratio on demand arbitrarily.For example: solid-liquid ratio can be 1: 8,1: 10, and 1: 100,1: 200 etc.
It is very little that material extracts forward and backward temperature increasing degree at high-pressure pulse electric, generally in 0～30 ℃.This method is suitable for industrial continuous production, has that extraction rate is fast, extraction ratio is high, the advantage of efficient energy-saving.
Description of drawings
Fig. 1 is the influence curve figure of high-pressure pulse electric field intensity to rapid extraction Semen sojae atricolor liposoluble substance;
Fig. 2 is that the high-pressure pulse electric pulse number is to extracting the influence curve figure of Semen sojae atricolor liposoluble substance;
Fig. 3 is different solvents rectangular histogram that influences to Semen sojae atricolor liposoluble substance extraction ratio under the inventive method;
The specific embodiment
By following examples and style interpretation of result thereof the inventive method is described in further detail
Embodiment 1.
To extract the Semen sojae atricolor liposoluble constituent is example, and the field intensity that describes high-pressure pulse electric in detail is to extracting the influence of liposoluble constituent.
After Semen sojae atricolor pulverized, add in the petroleum ether solution, be made into solid-liquid ratio and be 1: 10 mixed solution.This test is divided into 6 groups, and the test label is respectively A1, A2, A3, A4, A5, A6.Above-mentioned 5 groups of samples do not have through the impulse electric field processing except that the A1 sample, and by high voltage pulse electric field processing, treatment conditions are as follows respectively for other 5 groups of samples:
A1: do not have through impulse electric field and handle, 22 ℃ of lixiviates 24 hours; A2: field intensity 2kV/cm, 1 pulse; A3: field intensity 5kV/cm, 1 pulse; A4: field intensity 25kV/cm, 1 pulse; A5: field intensity 50kV/cm, 1 pulse; A6: field intensity 60kV/cm, 1 pulse.
With the material centrifugalize that high voltage pulse electric field processing is crossed, get supernatant, fat-soluble solvent is vapored away, the quality that takes by weighing surplus materials is come the content of liposoluble constituent in the Detection and Extraction liquid.Experimental result is with reference to Fig. 1.
From extracting the result as can be seen: 1) content of the liposoluble constituent of process this patent method extraction is all greater than the content that does not extract through this patent method; 2) extraction rate is fast, is a pulse, and a pulse only is 2 μ s, and promptly the time only is 2 μ s; 3) optimum electric field intensity of the inventive method is about 5kv/cm, and too high or too low liposoluble constituent extracted amount all has minimizing.
Embodiment 2
To extract the Semen sojae atricolor liposoluble constituent is example, describes the influence that the high-pressure pulse electric pulse number extracts liposoluble constituent in detail.
After Semen sojae atricolor pulverized, add in the oil fan solution, be made into solid-liquid ratio and be 1: 10 mixed solution.This test is divided into 6 groups, and the test label is respectively B1, B2, B3, B4, B5, B6.Above-mentioned 6 groups of samples do not have through the impulse electric field processing except that the B1 sample, and by high voltage pulse electric field processing, treatment conditions are as follows respectively for other 5 groups of samples:
B1: do not have through impulse electric field and handle, 22 ℃ of lixiviates 24 hours; B2:5 pulse, field intensity 25kV/cm; B3:30 pulse, field intensity 25kV/cm; B4:60 pulse, field intensity 25kV/cm; B5:200 pulse, field intensity 25kV/cm; B6:300 pulse, field intensity 25kV/cm.
With the material centrifugalize that high voltage pulse electric field processing is crossed, get supernatant, fat-soluble solvent is vapored away, the quality that takes by weighing surplus materials is come the content of liposoluble constituent in the Detection and Extraction liquid.Experimental result is with reference to Fig. 2.
Can find out from extracting the result: 1) along with the increase of pulse number, extracted amount increases: 2) be increased to after 60 when umber of pulse, extracted amount no longer increases.
Embodiment 3.
To extract the Semen sojae atricolor liposoluble constituent is example, describes different solvents extracts liposoluble constituent to high-pressure pulse electric influence in detail.
After Semen sojae atricolor pulverized, add in the different solvents, be made into solid-liquid ratio and be 1: 10 solution.This test is divided into 4 groups, and the test label is respectively C1, C2, C3, C4.Above-mentioned sampling test condition is as follows:
C1: solvent (acetone), high-pressure pulse electric (25kV/cm, 5 pulses);
C2: solvent (95% petroleum ether, 5% acetone mixed liquor), high-pressure pulse electric (25kV/cm, 5 pulses);
C3: solvent (petroleum ether), high-pressure pulse electric (25kV/cm, 5 pulses);
C4: solvent (ether), high-pressure pulse electric (25kV/cm, 5 pulses).
With the material centrifugalize of handling, get supernatant, fat-soluble solvent is vapored away, the quality that takes by weighing surplus materials is come the content of liposoluble constituent in the Detection and Extraction liquid.Experimental result is with reference to Fig. 3.
Can find out from extracting the result: the different extracted amounts of solvent have difference the bright method of we, and are maximum with the acetone solvent extracted amount, and the ether extraction amount is minimum.
Embodiment 4
To extract the corn germ liposoluble constituent is example, illustrates to use the inventive method to extract the corn germ liposoluble constituent.
After corn germ pulverized, add in the petroleum ether solution, be made into solid-liquid ratio and be 1: 10 mixed solution.This test is divided into 2 groups, and the test label is respectively D1, D2.D1 sample in above-mentioned 2 groups of samples (solvent extraction 24 hours) does not have through impulse electric field to be handled, and the D2 sample is by high voltage pulse electric field processing, and treatment conditions are: D2:30 pulse, field intensity 15kV/cm.
With the material centrifugalize that high voltage pulse electric field processing is crossed, get supernatant, fat-soluble solvent is vapored away, the quality that takes by weighing surplus materials is come the content of liposoluble constituent in the Detection and Extraction liquid.Experimental result is: the content of sample D1 number extraction liposoluble constituent is 43.44%; The content that extracts liposoluble constituent for sample D2 number is 45.57%.
Embodiment 5
Use the inventive method to extract mandarin oil.
After dried Pericarpium Citri tangerinae pulverized, add in the petroleum ether solution, be made into solid-liquid ratio and be 1: 10 mixed solution.By high voltage pulse electric field processing, its treatment conditions are: 5 pulses, field intensity 15kV/cm.Its mandarin oil extracted amount is 6.6%.
Embodiment 6
Use the inventive method to extract the Semen arachidis hypogaeae liposoluble constituent.
After Semen arachidis hypogaeae pulverized, add in the petroleum ether solution, be made into solid-liquid ratio and be 1: 10 mixed solution.By high voltage pulse electric field processing, its treatment conditions are: 5 pulses, field intensity 15kV/cm.Its liposoluble constituent extracted amount is 46.7%.
Embodiment 7
Use the inventive method to extract the Inonqqus obliquus liposoluble constituent.
After Inonqqus obliquus pulverized, add in the petroleum ether solution, be made into solid-liquid ratio and be 1: 10 mixed solution.By high voltage pulse electric field processing, its treatment conditions are: 5 pulses, field intensity 15kV/cm.Its liposoluble constituent extracted amount is 3.35%.
Embodiment 8
Use the inventive method to extract the Radix Ginseng liposoluble substance.
After Radix Ginseng pulverized, add in the petroleum ether solution, be made into solid-liquid ratio and be 1: 10 mixed solution.By high voltage pulse electric field processing, its treatment conditions are: 5 pulses, field intensity 15kV/cm.Its liposoluble substance extracted amount is 3.10%.
Embodiment 9
Use the inventive method to extract the bone tankage liposoluble substance.
To add petroleum ether solution in the bone tankage, be made into solid-liquid ratio and be 1: 10 mixed solution.By high voltage pulse electric field processing, its treatment conditions are 5 pulses, field intensity 15kV/cm.Its liposoluble substance extracted amount is 2.6%.
Claims (3)
1. the method for a rapid extraction liposoluble constituent from biological material, be to be raw material with the plant, animal or the mushroom biological material powder body that contain liposoluble constituent, with nonconducting fat-soluble organic solution as solvent, it is characterized in that it being that mixed solution with described powder material and solvent passes through high voltage pulse electric field processing at normal temperatures, its electric field intensity is that 2～60kV/cm, umber of pulse are 1～300, with the material centrifugalize behind the high voltage pulse electric field processing, get supernatant, solvent is removed in volatilization, promptly obtains solid-state liposoluble constituent.
2. according to claim 1 a kind of from biological material the method for rapid extraction liposoluble constituent, it is characterized in that described solvent is petroleum ether, ether, acetone or normal hexane etc.
3. according to claim 1 a kind of from biological material the method for rapid extraction liposoluble constituent, it is characterized in that described raw material is Semen sojae atricolor, Semen arachidis hypogaeae, Semen Sesami, bone meal, Fructus Hippophae, Pericarpium Citri tangerinae, Radix Ginseng, Inonqqus obliquus etc.
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| CNA2006100168563A CN1879731A (en) | 2006-05-18 | 2006-05-18 | A method for rapidly extracting fat soluble component from biological material |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102153504A (en) * | 2011-01-19 | 2011-08-17 | 长沙华康生物技术开发有限公司 | Method for preparing 1-deoxynojirimycin in mulberry leaves |
| CN107951964A (en) * | 2017-12-12 | 2018-04-24 | 山东金胜粮油集团有限公司 | A kind of peanut red coat extract and grape seed extract compounding capsule and its production method |
| RU2692375C1 (en) * | 2018-07-03 | 2019-06-24 | федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный медицинский университет" Министерства здравоохранения Российской Федерации | Device for production of extract of sophora japonica |
-
2006
- 2006-05-18 CN CNA2006100168563A patent/CN1879731A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102153504A (en) * | 2011-01-19 | 2011-08-17 | 长沙华康生物技术开发有限公司 | Method for preparing 1-deoxynojirimycin in mulberry leaves |
| CN102153504B (en) * | 2011-01-19 | 2013-10-30 | 长沙华康生物技术开发有限公司 | Method for preparing 1-deoxynojirimycin in mulberry leaves |
| CN107951964A (en) * | 2017-12-12 | 2018-04-24 | 山东金胜粮油集团有限公司 | A kind of peanut red coat extract and grape seed extract compounding capsule and its production method |
| CN107951964B (en) * | 2017-12-12 | 2021-02-09 | 山东金胜粮油集团有限公司 | Peanut skin extract and grape seed extract compound capsule and production method thereof |
| RU2692375C1 (en) * | 2018-07-03 | 2019-06-24 | федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный медицинский университет" Министерства здравоохранения Российской Федерации | Device for production of extract of sophora japonica |
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