CN1858068A - Method for preparing pollen pini polyose, pollen pipe polyose and its use in medicine - Google Patents
Method for preparing pollen pini polyose, pollen pipe polyose and its use in medicine Download PDFInfo
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Abstract
The process of preparing pollen pini polyose includes the following steps: 1. decocting wall broken pollen pini to extract and filtering; 2. enzymolyzing with pancreatin and cellulose, centrifuging at high speed and ultrafiltering to eliminate impurity; 3. alcohol precipitating the filter residue liquid, and drying the precipitate to obtain coarse pollen pini polyose; 4. eluting coarse pollen pini polyose in DEAE-cellulose column; and 5. nanofiltering, concentrating the filter residue liquid, drying and crushing to obtain single pollen pini polyose. The present invention also discloses the medicinal application in preparing medicine for lowering blood fat, blood pressure and blood sugar, resisting fatigue and raising immunity. The present invention provides one new way for developing and utilizing pollen pini.
Description
Technical field
The present invention relates to pine pollen polysaccharide and preparation method thereof, with and the application in the medicine of preparation reducing blood-fat, hypotensive, hypoglycemic, antifatigue and raising immunizing power of application, especially pine pollen polysaccharide in pharmacy.
Background technology
Along with the fast development of China's economic society, the improving constantly of living standards of the people, the consequent is the change of people's food habits, the increasing of bad habit, the increasing the weight of etc. of the quickening of work rhythm and social pressures.These factors cause ubiquity on people's health the problem of sub-health state, such as too fatigue, old and feeble quickening, immunity degradation, hypertension, hyperlipidemia, hyperglycemia etc.This is easy to bring out and resembles that cerebral thrombosis, hematencephalon, coronary heart disease, diabetes etc. are difficult thoroughly cures and the very big disease of sudden harm.More and more higher and sicken age is tending towards rejuvenation along with the morbidity of this class cardiovascular and cerebrovascular diseases, and this causes great threat to human beings'health, has also strengthened social burden.
Pollen Pini, Shennong's Herbal before two thousand years is just on the books, and it is called " pine is yellow ", describes its function in addition and be in Compendium of Material Medica: " preserved egg another name pine Huang; sweet; temperature, nontoxic profit cardiopulmonary, beneficial gas; the hemostasis of dispeling the wind; also can make wine " is the treasure of health care, and ancient times was the tribute in imperial palace once just.
The inventor has carried out research and experiment repeatedly by the extracting method to pine pollen polysaccharide, purification obtains the high extract of pine pollen polysaccharide thing content, and show with the long-term experiment result through groping repeatedly, pine pollen polysaccharide has regulating blood fat, blood pressure, blood sugar effect, also have antifatigue simultaneously, improve the function of immunizing power.The relevant effect of these pine pollen polysaccharides there is no to be reported.
Summary of the invention
One of purpose of the present invention is to provide a kind of preparation method of pine pollen polysaccharide.
Second purpose of the present invention also is to provide a kind of pine pollen polysaccharide.
The purposes that provides a kind of pine pollen polysaccharide new is provided three of purpose of the present invention.
For realizing first goal of the invention of the present invention, the present invention adopts following technical solution, a kind of preparation method of pine pollen polysaccharide, and this method comprises following process:
(1), extracts:, filter the Pollen Pini water boiling and extraction of broken wall;
(2), removal of impurities: through pancreatin and cellulase degradation, high speed centrifugation and ultrafiltration removal of impurities;
(3), alcohol precipitation: filtered solution ethanol alcohol precipitation not, precipitation is dry, the Pollen Pini Crude polysaccharides;
(4), wash-out: with Pollen Pini Crude polysaccharides wash-out on DEAE-Mierocrystalline cellulose pillar;
(5), drying: the elutriant nanofiltration, not filtered solution be concentrated into dried, drying, through pulverize the Pollen Pini single polysaccharide.
Described leaching process can for: get broken masson pine pollen, add the water of 25～30 times of amounts, in the heating extractor, stirring heating is warming up to 90 ℃～105 ℃, and constant temperature stirs and extracts 8～12h, is cooled to 40～48 ℃ then, filters.
Described removal of impurities process can be regulated pH to 6.5～8.5 with alkali for: filtrate, the pancreatin that adds Pollen Pini weight 1%～5%, 40～48 ℃ of insulation enzymolysis 4～8 hours, heat temperature raising to 95～105 ℃, at least kept 15 minutes, reduce to room temperature then,, add Pollen Pini weight 1%～5% cellulase with acid for adjusting pH 4.5～6.0, be incubated enzymolysis 4～8 hours at 40～48 ℃, reheat is warming up to 95～105 ℃, keeps at least 15 minutes, reduces to room temperature; Using the relative centrifugal force high speed centrifugation of 9000～30000g then, collect supernatant liquor, is the ultra-filtration membrane ultrafiltration of 10～100kd with the molecular weight that dams, and collects not filtered solution.
Described alcohol precipitation process can for: to be concentrated into relative density be not 1.05～1.2 to filtered solution, and transferring to concentration with ethanol is 65%～80% ethanolic soln, 4 ℃ of alcohol precipitation 12～18h, centrifugal, collecting precipitation is after washing with alcohol, the reduced vacuum drying gets the Pollen Pini Crude polysaccharides.
Described elution process can for: the Pollen Pini Crude polysaccharides is water-soluble, and be injected in the DEAE-Mierocrystalline cellulose, wash decon earlier with water, use 0.5～1mol/LNaOH eluant solution then, collect the NaOH elutriant, with acid for adjusting pH to 6.0～7.5.
The drying means of pine pollen polysaccharide extract is vacuum-drying, lyophilize, spraying drying, microwave drying etc., is preferably vacuum-drying or lyophilize.
The present invention also provides a kind of pine pollen polysaccharide, be adopt aforesaid method with broken masson pine pollen through water is carried, removal of impurities, alcohol precipitation, wash-out, drying obtain product.
The present invention also provides the new purposes of pine pollen polysaccharide.
A kind of pine pollen polysaccharide is in the application of pine pollen polysaccharide in the medicine of preparation lowering blood glucose.
The application of a kind of pine pollen polysaccharide in the medicine of preparation blood fat reducing.
The application of a kind of pine pollen polysaccharide in the medicine that preparation brings high blood pressure down.
The application of a kind of pine pollen polysaccharide in the medicine of preparation antifatigue.
The application of a kind of pine pollen polysaccharide in the medicine of preparation raising immunizing power.
Pine pollen polysaccharide is studied and experiment confirm repeatedly through the inventor, and its laboratory animal to hyperglycemia or hypertension or hyperlipidemia has good lowering blood glucose and blood pressure, blood fat.Simultaneously, experimentation on animals also shows, the effect that pine pollen polysaccharide has antifatigue and improves immune level.Can be as the medicine of the above-mentioned disease of treatment, or as one of effective ingredient of medicine.The extracting method of Pollen Pini sugar extract of the present invention, the content of its loose medicinal powder polysaccharide adopts phenol sulfuric acid process mensuration can reach more than 90%, be highly purified single pine pollen polysaccharide, changed the Pollen Pini exploitation, utilized mode to have very high economic and social benefit.
Embodiment
The invention will be further described below in conjunction with embodiment, do not limit scope of the present invention with this.
Embodiment 1: the preparation of pine pollen polysaccharide
(1), extract: get broken masson pine pollen 2kg, add 60L water, in the heating extractor, stirring heating is warming up to 95 ℃～100 ℃, and constant temperature stirs and extracts 8h, reduces to 40～45 ℃.
(2), removal of impurities: regulate pH to 8.0～8.5 with NaOH, add the pancreatin of Pollen Pini weight 3%, 40～45 ℃ of insulation enzymolysis 4 hours, heat temperature raising to 95 ℃ is kept 15min then, reduces to room temperature again; Regulate pH5.5～6.0 with HCl, add the cellulase of Pollen Pini weight 3%, 40～45 ℃ of insulation enzymolysis 4 hours, heat temperature raising to 95 ℃ is kept 15min, reduces to room temperature.With the relative centrifugal force high speed centrifugation of 9100g, collect supernatant liquor, be the ultra-filtration membrane ultrafiltration of 100kd with the molecular weight that dams, collect not filtered solution.
(3) alcohol precipitation: to be concentrated into relative density be not 1.1 for filtered solution heating, and transferring to concentration with ethanol is 75% ethanolic soln, and 4 ℃ of alcohol precipitation 16h are centrifugal, collecting precipitation, after washing with alcohol, 60 ℃ of reduced vacuum dryings, the Pollen Pini Crude polysaccharides.
(4) wash-out: the Pollen Pini Crude polysaccharides is water-soluble, and be injected in the DEAE-Mierocrystalline cellulose, wash decon earlier with water, use 1mol/L NaOH eluant solution then, collect the NaOH elutriant, regulate pH to 6.5～7.0 with HCl solution
(5) drying: nanofiltration, not filtered solution be concentrated into dried, lyophilize, through pulverize the one-component pine pollen polysaccharide, its content is 91.2%.
Embodiment 2: pine pollen polysaccharide is to the influence of renal hypertensive rat blood pressure level
1, laboratory animal and group setting
The Wistar rat, 9～10 ages in week, body weight 200 ± 20g, male, be divided into the blank group at random, and model control group and experiment medicine I group, II are organized 10 every group.
2, hypertensive rat model preparation
With diazepam 5mg/kg and ketamine 50mg/kg, the intracutaneous anesthetized rat, dorsal position is fixed, behind the skin degerming under xiphoid-process 1.5cm, cut skin and flesh layer along ventrimeson, extrude kidney gently, encase with being soaked with the physiological saline cotton, be fixed with left index finger and thumb then.The right hand separates the Renal artery with Wugou straight peen ophthalmology tweezer careful separation kidney the base of a fruit manadesma along the Renal vein below, nearly aorta end with H shape silver brain clip (internal diameter is 0.20～0.25mm) to put, with CN, layering suture operation otch at last.Choose systolic pressure＞160.15mmHg person after 4～5 weeks as the renovascular hypertension rat.
3, experimental technique
(1), the normal rat of pine pollen polysaccharide test
Normal rat is divided into 3 groups at random, every group 10, experiment medicine I group, II group gavage pine pollen polysaccharide (pressing method preparation among the embodiment 1) 100mg/kg and 300mg/kg respectively, irritate the stomach amount and be 10ml/kg, the blank group gavages equivalent physiological saline, successive administration 15d, the experimental session animal drinks water and ingests arbitrarily.Before the pressure measurement rat is put into 37 ± 1 ℃ of electrothermostats and heat, behind the 15min, measure rat caudal artery systolic pressure with the rat blood pressure determinator, experiment is respectively carried out once when beginning and finishing, and surveys 3 times continuously and averages as measured value.
(2), pine pollen polysaccharide is to the effect of hypertension model rat
Normal rat is divided into 4 groups at random, every group 10, wherein test medicine I group, II group and model control group making hypertension model, experiment medicine I organizes, the administration of II group is the same, blank group and model control group gavage equivalent physiological saline, successive administration 15d, the experimental session animal drinks water and ingests arbitrarily.Before the pressure measurement rat is put into 37 ± 1 ℃ of electrothermostats and heat, behind the 15min, measure rat caudal artery systolic pressure with the rat blood pressure determinator, experiment is respectively carried out once when beginning and finishing, and surveys 3 times continuously and averages as measured value.
4, result and analysis
4.1 influence to normal rat blood pressure
Experiment medicine I, the blood pressure of II group and the comparison of blank group, descend obviously (P＜0.05 and P＜0.01) illustrates that pine pollen polysaccharide has the reduction effect to the blood pressure of intact animal, sees Table 1.
Table 1 pine pollen polysaccharide is to the influence of normal rat blood pressure
| Group | n | Blood pressure (mmHg) | |
| Before the administration | After the administration | ||
| The blank group | 10 | 131.14±16.34 | 132.02±15.75 |
| Experiment medicine I group | 10 | 132.79±15.30 | 122.15±15.20 # |
| Experiment medicine II group | 10 | 132.57±16.12 | 110.75±16.11 * |
Compare #P＜0.05, * P＜0.01 with the blank group
4.2 influence to hypertensive rat model
The group of modeling and blank group compare before the administration, P＜0.01 explanation hypertensive rat model manufacturing success; Test between medicine I group, II group administration front and back group and compare P, illustrate that pine pollen polysaccharide has the effect of tangible reduction hypertension model rat blood pressure respectively less than 0.05 and 0.01.See Table 2
Table 2 pine pollen polysaccharide is to the influence of hypertensive rat model blood pressure
| Group | n | Blood pressure (mmHg) | |
| Before the administration | After the administration | ||
| The blank group | 10 | 105.30±16.73 | 107.22±15.16 |
| Model control group | 10 | 159.28±24.61 ## | 161.37±11.39 |
| Experiment medicine I group | 10 | 157.69±15.65 ## | 146.84±13.72 * |
| Experiment medicine II group | 10 | 160.88±18.15 ## | 141.83±12.53 ** |
Compare * P＜0.05, * * P＜0.01 before and after the administration in the group; Compare ##P＜0.01 with the blank group;
Embodiment 3: pine pollen polysaccharide is to the influence of blood-lipid imbalance disease rat fat level
1. animal is selected and the group setting
With embodiment 2.
2. hyperlipemia model of rats preparation
Hyperlipemia model of rats adopts high lipid food to cause the hyperlipidaemia method.The high lipid food prescription is as follows: basal feed 86.3%, cholesterol 3%, lard 10%, Thyreostat I 0.2%, pig cholate 0.5% guarantee that each composition mixes.Gavaged for 2 weeks continuously, tail blood is got in the anesthesia of Sodital sodium solution, detects blood fat, proves model manufacturing success.
3. experimental technique
The blank group: not modeling, not administration gives equivalent physiological saline;
Model control group: modeling, not administration gives high lipid food every morning;
Experiment medicine I group, II group: modeling, give high lipid food morning, gavage pine pollen polysaccharide 100mg/kg and 300mg/kg afternoon respectively with method preparation among the embodiment 1, irritate the stomach amount and be 10ml/kg, continuous 14 days.Drug withdrawal 24 hours (fasting 16 hours), tail blood is got in the anesthesia of Sodital sodium solution, detects.
4. detection index
The serum chemistry index comprises total cholesterol (TC), triglyceride level (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-L).Reagent adopts Japanese Luo Shi reagent company product, measures with Hitachi's 7020 automatic biochemistry analyzers.
5. multifactor analysis of variance method is adopted in statistical study
6. result and analysis
Model control group and its serological index of blank group comparison all have significant difference, P＜0.001.In administration after 14 days, experiment medicine I group, II group compare with model control group, serum total cholesterol, triglyceride level, low-density lipoprotein cholesterol level all significantly reduce, the high density lipoprotein cholesterol level is gone up to some extent, P is all less than 0.001, this shows that pine pollen polysaccharide all has remarkable reduction effect to serum total cholesterol, triglyceride level, low-density lipoprotein cholesterol level, can improve the high density lipoprotein cholesterol level simultaneously, sees Table 3.
Table 3 pine pollen polysaccharide is to the influence of rat model blood fat
| Group | TC(mmol/L) | TG(mmol/L) | LDL-C (mmol/L) | HDL-L (mmol/L) |
| The blank group | 4.35±0.86 | 0.61±0.13 | 2.83±0.34 | 0.96±0.18 |
| Model control group | 13.85±2.06 # | 1.63±0.34 # | 9.72±1.18 # | 0.42±0.13 # |
| Experiment medicine I group | 6.89±1.60 * | 0.61±0.18 * | 3.31±0.50 * | 0.98±0.35 * |
| Experiment medicine II group | 6.52±1.72 * | 0.57±0.12 * | 3.31±0.50 * | 1.22±0.20 * |
Annotate: compare with the blank group, * P＜0.001 is compared with model control group in #P＜0.001
Embodiment 4, pine pollen polysaccharide are to the influence of tetraoxypyrimidine rat model glucose level
1, laboratory animal is selected and the group setting
Kunming mouse, in 6 ages in week, body weight 20 ± 2.0g is divided into blank group, model control group, experiment medicine I group and experiment medicine II group at random, and 10 every group, male and female half and half.
2, the making of tetraoxypyrimidine mould mouse model
After the mouse fasting 12 hours,, inject to mouse peritoneal, bring out diabetes animal model by 120mg/kg with the 10mg/ml tetraoxypyrimidine solution of promptly preparing.Note observing the mouse state on modeling that night and second day, the mouse that first aid because of hypoglycemia spasm takes place is obviously promptly only irritated stomach 0.2ml/ with saturated glucose solution.
3, experimental technique
(1), respectively organizes mouse before the experiment and survey fasting blood sugar as follows.
(2), measure after, except that the blank group, all the other each groups are made the tetraoxypyrimidine mouse model as stated above, the then fasting blood sugar of rating model again is if concentration is greater than 11mmol/L then can determine model manufacturing success.Beginning administration in second day, experiment medicine I group, II group gavage pine pollen polysaccharide 100mg/kg and 300mg/kg respectively, irritate the stomach amount and are 20ml/kg; Blank group and model control group do not give medicine, gavage equivalent physiological saline, continuous 15 days, get glucose content in the hematometry serum.
4, detection method
(1), the mouse intraocular corner of the eyes gets blood, 4 ℃ left standstill one hour, centrifugal 10 minutes of 4 ℃ then, 3000rpm separate upper serum, with glucose content in the determination of glucose oxidase serum.The Reagent kit of glucose that reagent adopts Shanghai Rongsheng Bioisystech Co., Ltd to produce, instrument Hitachi's 7020 automatic biochemistry analyzers.
(2), the t check is adopted in statistical study.
4, result and analysis
Blood sugar detection value between each experimental group between each group of preceding fasting plasma glucose measured value of experiment and modeling, P＞0.05, no significant difference has comparability; Interior relatively its P value of group has significant difference all less than 0.01 before and after the administration of experiment medicine I, II group, illustrates that pine pollen polysaccharide has the effect of lowering blood glucose, the results are shown in Table 4.
Table 4 pine pollen polysaccharide is to the influence of tetraoxypyrimidine model mice glucose level
| Group | Blood sugar concentration (mmol/L) | P | ||
| Fasting plasma glucose | Before the administration | After the administration | ||
| The blank group | 4.38±0.96 | 4.80±1.12 | 4.16±0.75 | ＞0.05 |
| Model control group | 4.63±0.85 | 12.29±1.19 | 11.93±1.18 | ＞0.05 |
| Experiment medicine I | 4.53±0.95 | 12.45±1.47 | 10.13±1.13 | ＜0.01 |
| Group | ||||
| Experiment medicine II group | 4.69±1.52 | 11.33±1.19 | 8.64±1.32 | ＜0.01 |
Embodiment 5, mouse swimming with a load attached to the body test
1, experimental technique
Kunming mouse, in 6 ages in week, body weight 20 ± 2g is divided into blank group, experiment medicine I group and experiment medicine II group at random, and 10 every group, male and female half and half.Experiment medicine I group and experiment medicine II group respectively by every day every 100mg/kg and 300mg/kg gavage pine pollen polysaccharide respectively, the blank group gives the equivalent distilled water, irritates the stomach amount and is 20ml/kg, continuous 15 days.Behind the last administration 1h, every mouse afterbody bears a heavy burden (8% body weight), puts into 22 ℃ of water temperatures, in 80 * 60 * 40cm tank, and record mouse swimming time.
2, result and analysis
Experiment medicine I group and II group all can obviously prolong mouse swimming time (seeing the following form), show that pine pollen polysaccharide can obviously strengthen the ability of mouse anti-reflecting fatigue, the results are shown in Table 5.
Table 5 pine pollen polysaccharide is to the influence of mouse swimming with a load attached to the body time
| Group | Swimming time (min) |
| The blank group | 20.35±11.27 |
| Experiment medicine I group | 30.86±10.36 # |
| Experiment medicine II group | 41.21±14.11 * |
Compare #P＜0.05, * P＜0.01 with the blank group;
Embodiment 6, pine pollen polysaccharide are to the influence of mouse immune organ weight and reticuloendothelial system phagocytic function
1, pine pollen polysaccharide is to mouse immune organ weight's influence
Get 30 of BALB/c mouse, body weight 20 ± 2g, grouping and administration be with embodiment 5, every day 1 time, continuously 7d.Behind last 1 administration 30min, disconnected neck is put to death, and wins thymus gland and spleen, weighs, and calculates thymus index and index and spleen index, the results are shown in Table 6.
Table 6 pine pollen polysaccharide is to mouse immune organ weight's influence
| Group | Thymus index (mg/10g) | Index and spleen index (mg/10g) |
| The blank group | 26.54±6.34 | 39.20±10.83 |
| Experiment medicine I group | 32.91±5.77# | 46.37±7.70# |
| Experiment medicine II group | 40.64±7.62* | 53.64±9.78* |
Annotate: compare with the blank group, * P＜0.01 is compared with model control group in #P＜0.05
2.4 pine pollen polysaccharide is to the influence of mouse reticuloendothelial system phagocytic function
Get 30 of BALB/c mouse, body weight 20 ± 2g, grouping and administration be with embodiment 5, every day 1 time, continuously 7d.Behind last 1 administration 30min, tail vein injection india ink (with 5 times of physiological saline dilutions) 0.1ml/10g body weight is got blood 25ul respectively at the mouse orbit venous plexus when injection 2,12min, place 2ml0.1%Na
2CO
3In the solution test tube, shake up, with 0.1%Na
2CO
3Be blank, in 600nm place colorimetric, measure optical density(OD) (OD), calculate and clean up index k with spectrophotometer.The result shows that pine pollen polysaccharide I group, II group have obvious enhancement to normal mouse reticuloendothelial system phagocytic function, compares the P value respectively less than 0.05 and 0.01 with the blank group, has remarkable statistical significance, the results are shown in Table 7.
Table 7 pine pollen polysaccharide is to the influence of mouse reticuloendothelial system phagocytic function
| Group | Phagocytic index |
| The blank group | 0.0039±0.0020 |
| Experiment medicine I group | 0.0068±0.0024 # |
| Experiment medicine II group | 0.0084±0.0021 * |
Annotate: compare with the blank group, * P＜0.01 is compared with model control group in #P＜0.05.
Claims (10)
1, a kind of preparation method of pine pollen polysaccharide is characterized in that, this method comprises following process successively:
(1), extracts:, filter the Pollen Pini water boiling and extraction of broken wall;
(2), removal of impurities: through pancreatin and cellulase degradation, high speed centrifugation and ultrafiltration removal of impurities;
(3), alcohol precipitation: filtered solution ethanol alcohol precipitation not, precipitation is dry, the Pollen Pini Crude polysaccharides;
(4), wash-out: with Pollen Pini Crude polysaccharides wash-out on DEAE-Mierocrystalline cellulose pillar.
(5), drying: the elutriant nanofiltration, not filtered solution be concentrated into dried, drying, through pulverize the Pollen Pini single polysaccharide.
2, the preparation method of pine pollen polysaccharide according to claim 1, it is characterized in that, described leaching process is: get broken masson pine pollen, the water that adds 25～30 times of amounts, in the heating extractor, stirring heating is warming up to 90 ℃～105 ℃, and constant temperature stirs and extracts 8～12h, be cooled to 40～48 ℃ then, filter.
3, the preparation method of pine pollen polysaccharide according to claim 1, it is characterized in that, described removal of impurities process is: filtrate is regulated pH to 6.5～8.5 with alkali, the pancreatin that adds Pollen Pini weight 1%～5%, 40～48 ℃ are incubated enzymolysis 4～8 hours, heat temperature raising to 95～105 ℃, at least kept 15 minutes, reduce to room temperature then,, add Pollen Pini weight 1%～5% cellulase with acid for adjusting pH 4.5～6.0, be incubated enzymolysis 4～8 hours at 40～48 ℃, reheat is warming up to 95～105 ℃, keeps at least 15 minutes, reduces to room temperature; Using the relative centrifugal force high speed centrifugation of 9000～30000g then, collect supernatant liquor, is the ultra-filtration membrane ultrafiltration of 10～100kd with the molecular weight that dams, and collects not filtered solution.
4, the preparation method of pine pollen polysaccharide according to claim 1, it is characterized in that, described alcohol precipitation process can for: to be concentrated into relative density be not 1.05～1.2 to filtered solution, transferring to concentration with ethanol is 65%～80% ethanolic soln, and 4 ℃ of alcohol precipitation 12～18h are centrifugal, collecting precipitation, after washing with alcohol, the reduced vacuum drying gets the Pollen Pini Crude polysaccharides.
5, the preparation method of pine pollen polysaccharide according to claim 1, it is characterized in that, described elution process is: the Pollen Pini Crude polysaccharides is water-soluble, and be injected in the DEAE-Mierocrystalline cellulose, wash decon earlier with water, use 0.5～1mol/L NaOH eluant solution then, collect the NaOH elutriant, with acid for adjusting pH to 6.0～7.5.
6, a kind of pine pollen polysaccharide is characterized in that: any one described method prepares in this extract employing claim 1 to 5.
7, a kind of pine pollen polysaccharide is preparing lowering blood glucose or/and the application in the medicine of blood fat.
8, the application of a kind of pine pollen polysaccharide in the medicine that preparation brings high blood pressure down.
9, the application of a kind of pine pollen polysaccharide in the medicine of preparation antifatigue.
10, the application of a kind of pine pollen polysaccharide in the medicine of preparation raising immunizing power.
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| CN101117354B (en) * | 2007-09-04 | 2011-01-19 | 山东师范大学 | Esterified pine pollen polysaccharide and preparation method and use thereof |
| CN101411727B (en) * | 2008-12-01 | 2011-09-28 | 浙江亚林生物科技股份有限公司 | Formula of pine pollen essence tablet and preparation technique thereof |
| CN101518552B (en) * | 2009-04-10 | 2012-01-04 | 南京师范大学 | Pine pollen and cyclodextrin composition, method for preparing same, use of same in preparation of health-care products for resisting fatigue and improving immunity of organisms |
| CN102372787A (en) * | 2010-08-10 | 2012-03-14 | 卢卫红 | Method for producing maize pollen polysaccharides with radiation-proof effect by membrane separation technology |
| CN102372787B (en) * | 2010-08-10 | 2014-06-11 | 卢卫红 | Method for producing maize pollen polysaccharides with radiation-proof effect by membrane separation technology |
| CN103130902A (en) * | 2011-11-29 | 2013-06-05 | 烟台新时代健康产业有限公司 | Method of extracting polysaccharide from pine pollen |
| CN103130902B (en) * | 2011-11-29 | 2015-04-22 | 烟台新时代健康产业有限公司 | Method of extracting polysaccharide from pine pollen |
| CN102746413A (en) * | 2012-07-16 | 2012-10-24 | 桐庐兴源保健品有限公司 | Method for preparing bee pollen polysaccharide through combining enzymolytic wall-breaking with hot-water ultrasonic extracting |
| CN103948018A (en) * | 2014-04-24 | 2014-07-30 | 邱银平 | Double-mushroom, hericium erinaceus and pollen pini food and preparation method thereof |
| CN106135705A (en) * | 2016-08-18 | 2016-11-23 | 广州暨南生物医药研究开发基地有限公司 | A kind of health pig feed additive and preparation and application thereof |
| CN108902286A (en) * | 2018-07-23 | 2018-11-30 | 郑州成济堂生物科技有限公司 | A kind of preparation method of rainbow trout composite preservative |
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