CN1827765A - Screening method for anaerobe capable of degrading chlorophenol - Google Patents
Screening method for anaerobe capable of degrading chlorophenol Download PDFInfo
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- CN1827765A CN1827765A CNA2006100235356A CN200610023535A CN1827765A CN 1827765 A CN1827765 A CN 1827765A CN A2006100235356 A CNA2006100235356 A CN A2006100235356A CN 200610023535 A CN200610023535 A CN 200610023535A CN 1827765 A CN1827765 A CN 1827765A
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Abstract
The method uses the anaerobic glove case and many modified culture medium to screen the anaerobic bacteria which possesses the highly effective character of degrading chlorophenol and acid resistance. The method comprises the following steps: A. screening the anaerobic particle sludge; B. segregation and purifying bacteria; C. primary screening the anaerobic bacteria degrading chlorophenol; D. second screening: segregation and purifying with the solid-liquid culture medium, until the cell shape being the single thallus. The operation is simple, and the theory meaning and economic value are important. The method provides the theory basis for anaerobic biological treatment process of chlorophenol waste water. The method can be used to separate, screen and culture the chlorophenol degradation bacterium, and provide the good screening method for researching chlorophenol degradation rule and interaction between function bacteria.
Description
Technical field
The present invention relates to the anerobe method that a kind of screening has the degrading chlorophenol function.Belong to microorganism field.
Background technology
Chlorophenol is chlorinated hydrocarbon, sterilant or the antimicrobial reagent that a class is used for non-agricultural desinsection and uses.It only is 40ppm to the concentration threshold that human body produces toxic action, and the threshold value that fish is produced toxic action is 1ppm, and the chlorophenol of 1ppb can cause the pollution of water body in water body.Classified as priority pollutant (Priority Pollutants) and Chinese key pollutants Black List (Blacklist) by U.S. EPA.At present treatment processs such as the ultrafiltration process that the chlorophenols material is commonly used, chemical precipitation method, biological process exist that energy consumption is big, cost is high, medicament adds and easily causes secondary pollution.Therefore the Anaerobic Microbiological Treatment Technology of this hardly degraded organic substance thing of P-Chlorophenol will provide a selection for the degraded of hardly degraded organic substance.Though people have utilized the bio-reactor domestication and turned out the anaerobic sludge with degrading chlorophenol function, and obtain good effect of removing (70-90%), acclimation period is longer, the treatment effect instability.Have the anaerobic sludge and the unblooded chlorophenol degradation bacteria of degrading chlorophenol function after this domestication, and do not pass through primary dcreening operation and multiple sieve, therefore do not obtain the higher chlorophenol degradation bacteria of purity.Therefore can't science regulate the degradation technique of chlorophenols material under the anaerobic condition, obtain sufficient theoretical foundation in anaerobic technique operation aspect and technical director, reach promising result.As everyone knows, the growth needs strictly anaerobic environment of anaerobic bacterium, especially methanogen, surpass in redox potential-0.33V (promptly in one liter 30 ℃ water, the content of oxygen only 1.48 * 10
-55Molecule) can not grow the time.As seen, the living environment of anerobe requires very harsh, causes people to be difficult to separate or cultivate single bacterium colony anaerobism function yeast under laboratory condition.For the anerobe of cultivating and screening has certain poisonous hardly degraded organic substance pollutent function of degraded, people generally adopt " Hungate rolls the pipe method ", anaerobism cylinder method, anaerobism bag (Bio-bag) method, Jiao's property end infanticide acid system etc.But shortcomings such as these cultural method ubiquity complex operations, anaerobic environment are difficult for keeping.For example Jiao's property end infanticide acid system is to spread gauze or filter paper on the slide of a cleaning, evenly removes part of the body cavity above the diaphragm housing the heart and lungs end gallate-based, and then sneaks into NaHCO
3Powder or NaOH solution, with above the flat board of inoculated bacteria has tipped upside down on, the Chinese wax edge sealing with melting causes an enclosed space rapidly.Oxygen consumption behind Jiao's property end gallate-based and the alkali reaction.This method can be used for the cultivation of the undemanding anerobe of anaerobism, if any clostridium.Anaerobism bag (Bio-bag) promptly causes anaerobic environment to cultivate anerobe in plastics bag.Plastics bag is transparent and airtight, interior dress gas generating tube (sodium bicarbonate solid and 5% citric acid ampoule that sodium borohydride is arranged), U.S. blue indicator pipe, palladium catalyst pipe, siccative.After putting into the flat board of having inoculated, extrude a bag interior air as far as possible, seal sack then.The gas generating tube that fractures earlier, after the U.S. blue indicator pipe that fractures, bag causes air-free environment in inherent half an hour.As having reached anaerobic state in the expression bag that do not suddenly change, can hatch.Anaerobism glove box (Anaerobic Chamber) is one of best instrument of cultivation anerobe of generally acknowledging in the world up to now.It is an airtight large-scale metal box, and the transparent panel that the front of case has a synthetic glass to do is equipped with two gloves on the plate, can operate in case by gloves, so name.The case side has a switch room, has inside and outside two, is closing earlier in the interior gate open case.Desire to put the thing cartonning, open external door earlier, put into the switch room, shut external door and bleed and take a breath (H
2, CO
2, N
2) reaching anaerobic state, hand stretches into gloves switch room's inside door is opened then, and article are moved in the case, closes upper internal door.Keeping anaerobic state in the case, also is to utilize hydrogen in the inflation under the catalysis of palladium and the principle that the money residual oxygen is combined to water in the case.But this case attemperation, itself be incubator, or incubator is within it attached, also can put into dissecting microscope and be convenient to observe the anerobe bacterium colony, this anaerobic box is suitable for doing a large amount of culture studies of anaerobic bacterium, and a large amount of substratum can be put into and make prereduction and anaerobism sterility test.The bleeding of metal sclerine type anaerobic box, inflation, anaerobic environment and temperature etc. are automatic adjusting.As can be seen, other methods,anaerobics are operated and can not screen required necessity because own characteristic generally only is used for cultivation and the observation of anerobe.Utilize the anaerobism glove box both can inoculate easily, cultivate, separate and screen, can observe microbial culture and growing state easily again.Therefore, compare, use cultivation of anaerobism glove box and screening to have remarkable advantages with other methods,anaerobics.
Summary of the invention
Purpose of the present invention discloses a kind of method that a strain has the anerobe of efficient degradation chlorophenol and acidproof feature that filters out easy and simple to handle.
For achieving the above object, growth conditions and chlorophenol domestication and degradation characteristic that the present invention is special according to anaerobic bacterium have adopted and can create that the anaerobism glove box of anaerobic environment separates and purification experiment with keeping preferably.The present invention carries out according to following step: the screening of A. anaerobic grain sludge: select the anaerobic sludge that color and luster is pitch-black, particle is bigger, filter out the granule sludge of diameter greater than 8mm with the 8mm hole sizer; The anaerobic grain sludge domestication of B, chlorophenol degraded: the anaerobic grain sludge in inoculation A step is tamed to the UASB anaerobic reactor; Biological flora adopts extension rate method and dull and stereotyped coating to determine the inoculum density of granule sludge bacterium liquid in the granule sludge that domestication is obtained, and carries out bacterium and separate and purifying; The primary dcreening operation of the anerobe of C, degrading chlorophenol: to separate and thalline that purifying obtains with physiological saline or aseptic distillation water dissolution, become bacterium liquid, get the 1-2ml mixed bacteria liquid and be inoculated on the liquid nutrient medium, enrichment culture wherein adds chlorophenol in the liquid nutrient medium; D, the multiple sieve of the anerobe of degrading chlorophenol: get bacterium liquid that primary dcreening operation obtains and be inoculated in the solid medium coating and cultivate, after waiting to grow thalline, choose colony inoculation in liquid nutrient medium with the sterilization pin, after treating to grow bacterial strain in the liquid nutrient medium, shake up bacterium liquid, 1-2ml bacterium liquid is inoculated into solid medium carries out identification of morphology, or carry out that microscopic examination and identification of cell form be whether identical judges whether success of separation and purification, if thalline is impure, continue to adopt the solid-liquid substratum to replace that cultured method is separated and purifying, up to identify from cellular form confirm as single thalline till.
Method of the present invention has following advantage and effect:
1. because the present invention uses current leading in the world and be known as best anaerobic bacterium culture apparatus-anaerobism glove box, therefore can inoculate easily, cultivate, separate and screen, can observe microbial culture and growing state easily again, compare the anerobe with degrading chlorophenol function that the present invention turns out with existing method purer.
2. because the present invention has adopted domestication of UASB reactor and cultivation to have the anaerobic grain sludge inoculation of degrading chlorophenol function, four kinds of improved anaerobic bacteria culture bases such as exploitation and use sodium thioglycollate, turn out anaerobic bacterium by primary dcreening operation and multiple sieve with degrading chlorophenol function, therefore cultivating domestication with existing other anaerobism compares with cultural method, technology is simple, it is easier to operate, and the bacterial population diversity has remarkable advantages.
3. physiological metabolism that the screening bacterial strain that adopts present method to obtain can be correlated with and degradation rule research, for the research of degrading chlorophenol function yeast provides the basis, more can provide theoretical foundation and technical guarantee for the anaerobic treatment process design and running mode of poisonous and harmful waste water such as optimization process chlorophenols.
4. on the population diversity basis relatively of the present invention bacterium in to the purebred chlorophenol degradation bacteria of various culture medium culturing and reactor, study the various function yeast that filter out P-Chlorophenol degradation rule and connecting each other and influence mechanism between them separately, using for the anaerobic biological treatment engineering of chlorophenol waste water provides theoretical foundation and guidance.
Description of drawings
Fig. 1 is the UASB reactor flow process synoptic diagram of laboratory scale domestication among the present invention and cultivation anaerobic grain sludge
Fig. 2 is a granule sludge sampler synoptic diagram
Fig. 3 is a 1029Thermo Forma Anaerobic System model anaerobism glove box synoptic diagram
Fig. 4 is chlorophenol concentration and bacterium liquid OD
600Curve is change curve in time, and wherein chlorophenol is the ortho position chlorophenol, and bacterium liquid is for having inoculated about 1.3 * 10
7Individual anerobe bacterium liquid with degrading chlorophenol function
The pure strain morphology that Fig. 5 goes out for the Saab culture medium culturing
Embodiment
Embodiment 1
See also Fig. 1,2 and 3.
Present embodiment is carried out according to following step:
The screening of A, anaerobic grain sludge: get the good granule sludge of quality from the anaerobic reactor of handling high concentrated organic wastewater such as citric acid wastewater, sieving discards floc sludge, keeps particle diameter greater than the 8mm granule sludge;
The anaerobic grain sludge domestication of B, chlorophenol degraded: adopt laboratory scale upflow type anaerobic granular sludge reactor (UASB), the good anaerobic grain sludge of quality that A step the is enriched to UASB that packs into cultivates and tames, glucose and trace element and somatomedin nutritive medium pump into UASB with peristaltic pump from the bottom, anaerobic grain sludge is tamed, the water bath with thermostatic control chuck water inlet of UASB is to control 35 ℃ of constant temperature by the heating rod and the temperature sensor that are provided with in the constant water bath box with temperature controller, pumps into chuck and carries out round-robin.Glucose mimic COD concentration range is at 0～2000mg/L, and the concentration of chlorophenol in water inlet is brought up to 100mg/L gradually from 0; As monitoring index, when COD clearance and chlorophenol degradation rate reached and maintain 80-90%, COD concentration remained unchanged, and improves the concentration of chlorophenol in water inlet with COD clearance and chlorophenol degradation rate; When COD clearance and chlorophenol degradation rate reach once more and maintain 80-90%, continue to improve chlorophenol concentration, reduce glucose concn, until being that single-matrix operation anaerobic reactor and chlorophenol degradation rate are more than 80% with the chlorophenol; Trace element that uses in the domestication process and somatomedin nutrient composition and concentration mg/L are: CaCl
22H
2O 100; CuSO
410; MgCl
26H
2O 200; FeSO
47H
2O 100; CoCl
26H
2O 25; MnSO
4H
2O 100; Boric acid 10; ZnSO
47H
2O 25; NiCl
26H
2O 20; (NH
4)
6Mo
7O
244H
2O10; NaCl 50; Yeast extract 10.
The primary dcreening operation of the anerobe of C, degrading chlorophenol: a. thalline that dull and stereotyped coating separation obtains to solid medium is with physiological saline or aseptic distillation water dissolution, with the homemade mud sampler of being made up of syringe and the rubber tubing that is connected with syringe (accompanying drawing 2) insertion reaction device granule sludge internal suction 1-3ml mud liquid; B, the mud sampler is moved into rapidly in the anaerobism glove box, then mud in the sampler is forwarded in the 10ml aseptic plastic centrifuge tube, fully stir mud liquid so that the granule sludge fragmentation with glass stick; The mud liquid of c, taking-up 1-2ml fragmentation is diluted to 10 successively
-1, 10
-2, 10
-3, 10
-4, 10
-5Constant gradient solution; D, get each gradient solution of 1-2ml and evenly be applied on the solid medium plate, to the thalline of growing on the plate with physiological saline or aseptic distillation water dissolution, get the 1-2ml mixed bacteria liquid and be inoculated into the liquid nutrient medium enrichment culture, wherein add the chlorophenol of 5-10mg/L concentration in the liquid nutrient medium; Observe the colony growth situation after 24-48 hour, finally determine 4 kinds of substratum are chosen the extension rate that is convenient to enumeration and observation.
The multiple sieve of the anerobe of D, degrading chlorophenol: get bacterium liquid 1-2ml that primary dcreening operation obtains and be inoculated in the solid medium coating and cultivate, after waiting to grow bacterium colony, choose colony inoculation in liquid nutrient medium with the sterilization pin, after treating to grow bacterial strain in the liquid nutrient medium, shake up bacterium liquid, get 1-2ml bacterium liquid and be inoculated into solid medium and carry out identification of morphology, judge whether success of separation and purification.Cultural method separates and purifying if the impure continuation employing of thalline solid-liquid substratum replaces.Wherein pour the 10-20ml solid medium into, clog bottleneck in order to avoid pollute with the bottled liquid nutrient medium of 100ml taper and with tampon and gauze with the 90cm glass dish; Bacterium by the above-mentioned steps purifying can the efficient degradation chlorophenol, the chlorophenol concentration and the bacterium liquid OD of a kind of pure bacterial strain P-Chlorophenol that Fig. 4 obtains for the Saab substratum (is example with the ortho position chlorophenol) degradation process
600(Optical Density) be change curve (inoculated bacteria number about 1.3 * 10 in time
7).Fig. 5 is the microphotograph of above-mentioned pure bacterial strain.
The four kinds of substratum and the composition thereof that adopt among the present invention are: the composition and the content g/L of a, sodium thioglycollate substratum are: trypticase 15; Sodium-chlor 2.5; Glucose 5; L-halfcystine 0.5; Sodium thioglycollate 0.5; Agar 20; Yeast decoction 5; PH is 7.1 ± 0.2.B, Saab substratum are formed and content g/L: peptone 10; Agar 20; Maltose 20-40; PH is 6.4 ± 0.2; C, Martin's culture medium prescription and content g/L: peptone 5; Dipotassium hydrogen phosphate 1; Yeast decoction 2; MgSO
47H
2O 0.5; Agar 20; PH value 6.8; D, Tongji University's substratum are formed and content g/L: glucose 10-20; Tryptones 4; Nutrient broth 2; Yeast decoction 1; NaCl 5; K
2HPO
41.5; MgCl
20.1; FeSO47H2O 0.1; L-halfcystine 0.5; Agar 20.Wherein solid medium is to add 20g/L agar on the liquid medium within composition basis to make.
The collection of A, anaerobic species: choose the deep soil (anerobe is brought into play main Degradation) that is subjected to the chlorophenols contaminated wastewater;
The anaerobic grain sludge domestication flow process of B, chlorophenol degraded is identical with embodiment 1.In 50-500ml glass wide-mouth reagent bottle, prepare mud storage liquid (liquid volume be reagent bottle 1/3) with preprepared deoxidation sterilized water, the chlorophenol of about 100mg is polluted deep soil pack into rapidly in the glass wide-mouth reagent bottle, seal bottleneck; Mud storage liquid composition can be with reference to following composition, g/L:NaHCO
3, 0.5; Sodium acetate, 0.5; NH
4HCO
3, 0.01; KH
2PO
4, 0.005; Yeast, 0.1; Mud sample is put into the anaerobism glove box, then mud in the sampler is forwarded in the 10ml aseptic plastic centrifuge tube, fully stir mud liquid so that sludge crushing is even with glass stick; Take out 1-2ml mud liquid and be diluted to 10 successively
-1, 10
-2, 10
-3, 10
-4, 10
-5Constant gradient solution; Getting the extension rate inoculum density solution that is convenient to enumeration and observation evenly is applied on the solid medium plate;
The primary dcreening operation of the anerobe of C, degrading chlorophenol: to the thalline of growing on the plate with physiological saline or aseptic distillation water dissolution, get the 1ml-5ml mixed bacteria liquid and be inoculated into the liquid nutrient medium enrichment culture, whether the chlorophenol that wherein adds the 0-50mg/L concentration gradient in the liquid nutrient medium finally determines that according to bacterial growth suitable chlorophenol adds concentration;
The multiple sieve of D, chlorophenol degradation bacteria: get bacterium liquid 1-2ml volume that primary dcreening operation obtains and be inoculated in the 90cm glass dish solid medium coating and cultivate, after waiting to grow bacterium colony, choose a colony inoculation in liquid nutrient medium with the sterilization pin.After treating to grow bacterial strain in the liquid nutrient medium, shake up bacterium liquid, get 1ml liquid and be inoculated into solid medium and carry out identification of morphology, judge whether success of separation and purification.If thalline is impure, continue to adopt the solid-liquid substratum to replace that cultural method separates and purifying.Wherein pour the 10-20ml solid medium into, clog bottleneck in order to avoid pollute with the bottled liquid nutrient medium of 100ml taper and with tampon and gauze with the 90cm glass dish; Four kinds of medium component see claim book the 3rd points that adopt among the present invention.
Claims (6)
1. the screening method of the anerobe of an energy degrading chlorophenol, it is characterized in that: it screens from anaerobic grain sludge, and carries out according to following step:
A. the screening of anaerobic grain sludge: select the anaerobic sludge that color and luster is pitch-black, particle is bigger, filter out the granule sludge of diameter greater than 8mm with the 8mm hole sizer.
The anaerobic grain sludge domestication of B, degrading chlorophenol: the anaerobic grain sludge in inoculation A step is tamed to the UASB anaerobic reactor, adopt extension rate method and the dull and stereotyped coating of solid medium to determine the inoculum density of granule sludge bacterium liquid to biological flora in the granule sludge that obtains after the domestication, and carry out bacterium and separate and purifying;
The primary dcreening operation of the anerobe of C, degrading chlorophenol: to separate and thalline that purifying obtains with physiological saline or aseptic distillation water dissolution, become bacterium liquid, bacterium liquid is inoculated on the liquid nutrient medium, enrichment culture wherein adds chlorophenol in the liquid nutrient medium;
D, the multiple sieve of the anerobe of degrading chlorophenol: get bacterium liquid that primary dcreening operation obtains and be inoculated in the solid medium coating and cultivate, after waiting to grow thalline, choose colony inoculation in liquid nutrient medium with the sterilization pin, after treating to grow bacterial strain in the liquid nutrient medium, shake up bacterium liquid, bacterium liquid is inoculated into solid medium carries out identification of morphology, or carry out that microscopic examination and identification of cell form be whether identical judges whether success of separation and purification, if thalline is impure, continue to adopt the solid-liquid substratum to replace that cultural method separates and purifying, up to identify from cellular form confirm as single thalline till.
2, the screening method of the anerobe of a kind of energy degrading chlorophenol according to claim 1 is characterized in that: the acclimation method of the anaerobic grain sludge of the chlorophenol degraded of described B in the step is achieved in that a, inoculation anaerobic grain sludge are to the UASB anaerobic reactor; B, domestication are initially, with glucose as altogether metabolism matrix and main carbon source, make glucose mimic COD concentration range at 0～2000mg/L, measure the concentration of chlorophenol in water inlet, bring up to 100mg/L gradually from 0 with the HP-1050 high performance liquid chromatograph that U.S. Hewlett-Packard Corporation produces; As monitoring index, when COD clearance and chlorophenol degradation rate reached and maintain 80-90%, COD concentration remained unchanged, and improves the concentration of chlorophenol in water inlet with COD clearance and chlorophenol degradation rate; When COD clearance and chlorophenol degradation rate reach once more and maintain 80-90%, continue to improve chlorophenol concentration, reduce glucose concn, until being that single-matrix operation anaerobic reactor and chlorophenol degradation rate are more than 80%, till the domestication degree reaches chlorophenol carried out complete dechlorination and biological degradation with the chlorophenol.
3, the screening method of the anerobe of a kind of energy degrading chlorophenol according to claim 1, it is characterized in that: the liquid/solid substratum in described D step is respectively sodium thioglycollate substratum, Saab substratum, Martin's substratum and Tongji University's substratum, and wherein solid medium is that the agar that adds 20g/L on the liquid medium within composition basis is made; Its composition is respectively: the composition and the content g/L of a, sodium thioglycollate substratum are: trypticase 15; Sodium-chlor 2.5; Glucose 5; L-halfcystine 0.5; Sodium thioglycollate 0.5; Agar 20; Yeast decoction 5; PH is 7.1 ± 0.2; B, Saab substratum are formed and content g/L: peptone 10; Agar 20; Maltose 20-40; PH is 6.4 ± 0.2; C, Martin's culture medium prescription and content g/L: peptone 5; Dipotassium hydrogen phosphate 1; Yeast decoction 2; MgSO
47H
2O 0.5; Agar 20; PH value 6.8; D, Tongji University's substratum are formed and content g/L: glucose 10-20; Tryptones 4; Nutrient broth 2; Yeast decoction 1; NaCl5; K
2HPO
41.5; MgCl
20.1; FeSO4.7H2O 0.1; L-halfcystine 0.5; Agar 20; Add 10ml VITAMIN liquid and 10ml liquid microelement in above-mentioned every liter of substratum, the composition of VITAMIN liquid and content mg/L are cobalamins 0.01; Xitix 0.025; Riboflavin 0.025; Citric acid 0.02; Folic acid 0.01; Sodium Benzoate 0.01; Creatine, 0.025; The composition of liquid microelement and content mg/L are MnSO
47H
2O 0.01; Zn
2SO
47H
2O 0.05; H
3BO
30.01; N (CH
2COOH)
34.5; CaCl
22H
2O 0.01; Na
2MoO
40101; CoCl
26H
2O 0.2.
4, the method for domestication degrading chlorophenol anaerobic grain sludge according to claim 2 is characterized in that: trace element that uses in the domestication process and somatomedin nutrient composition and concentration mg/L are: CaCl
22H
2O100; CuSO
410; MgCl
26H
2O 200; FeSO
47H
2O 100; CoCl
26H
2O 25; MnSO
4H
2O100; Boric acid 10; ZnSO
47H
2O 25; NiCl
26H
2O 20; (NH
4)
6Mo
7O
244H
2O 10; NaCl 50; Yeast extract 10.
5, the screening method of a kind of anerobe that can degrading chlorophenol according to claim 1 is characterized in that: the primary dcreening operation process in C step get method that anaerobic grain sludge in the reactor is inoculated into substratum be achieved in that a, with homemade mud sampler insertion reaction device granule sludge internal suction 1-3ml mud liquid; B, the mud sampler is moved into rapidly in the anaerobism glove box, then mud in the sampler is forwarded in the 10ml aseptic plastic centrifuge tube, fully stir mud liquid so that the granule sludge fragmentation with glass stick; C, the broken mud liquid of taking-up are diluted to 10 successively
-1, 10
-2, 10
-3, 10
-4, 10
-5Constant gradient solution; D, get each gradient solution and evenly be applied on the solid medium, observe the colony growth situation after 24-48 hour, finally determine 4 kinds of substratum are chosen the extension rate that is convenient to enumeration and observation.
6, the screening method of the anerobe of a kind of energy degrading chlorophenol according to claim 1, it is characterized in that: C with the operational condition of the screening process in D step is: the anaerobism glove box of a, employing 1029 Thermo Forma AnaerobicSystem models separates and screens operation, keeps N in the case
2: H
2: CO
2Ratio is 85: 10: 5, and oxygen content is lower than 1%; B, Resazurin or other anaerobism indicator characterize anaerobism purity in the case: oxygen concn reaches indicator variable color at once behind the certain level in the case; Dimension is kept 35 ℃ in c, the case.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101270334B (en) * | 2007-03-23 | 2011-04-20 | 中国科学院过程工程研究所 | Isolated culture method for anaerobic microorganism |
| CN102680164A (en) * | 2012-05-08 | 2012-09-19 | 中国科学技术大学 | On-line monitoring system for anaerobic reactor |
| CN104908063A (en) * | 2015-06-24 | 2015-09-16 | 中山安荞生物科技有限公司 | Vacuum glove box |
| CN105060507A (en) * | 2015-09-08 | 2015-11-18 | 天津商业大学 | Domestication kit and domestication method of strain for coal coking wastewater COD (chemical oxygen demand) degradation |
| CN106746436A (en) * | 2017-02-06 | 2017-05-31 | 同济大学 | A kind of method of raising L glucose anaerobic degradations |
| CN109502745A (en) * | 2018-12-10 | 2019-03-22 | 北京工业大学 | A method of quickly taming the microorganism of degradable 2,4,6- trichlorophenol |
| CN110606562A (en) * | 2019-09-23 | 2019-12-24 | 苏州科技大学 | Method for producing methane from high-concentration phenol-containing waste water |
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2006
- 2006-01-23 CN CNA2006100235356A patent/CN1827765A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101270334B (en) * | 2007-03-23 | 2011-04-20 | 中国科学院过程工程研究所 | Isolated culture method for anaerobic microorganism |
| CN102680164A (en) * | 2012-05-08 | 2012-09-19 | 中国科学技术大学 | On-line monitoring system for anaerobic reactor |
| CN102680164B (en) * | 2012-05-08 | 2013-12-18 | 中国科学技术大学 | On-line monitoring system for anaerobic reactor |
| CN104908063A (en) * | 2015-06-24 | 2015-09-16 | 中山安荞生物科技有限公司 | Vacuum glove box |
| CN105060507A (en) * | 2015-09-08 | 2015-11-18 | 天津商业大学 | Domestication kit and domestication method of strain for coal coking wastewater COD (chemical oxygen demand) degradation |
| CN105060507B (en) * | 2015-09-08 | 2020-02-28 | 天津商业大学 | Domestication kit and domestication method for strain for coal coking wastewater COD degradation |
| CN106746436A (en) * | 2017-02-06 | 2017-05-31 | 同济大学 | A kind of method of raising L glucose anaerobic degradations |
| CN106746436B (en) * | 2017-02-06 | 2020-06-26 | 同济大学 | Method for improving anaerobic degradation of L-glucose |
| CN109502745A (en) * | 2018-12-10 | 2019-03-22 | 北京工业大学 | A method of quickly taming the microorganism of degradable 2,4,6- trichlorophenol |
| CN109502745B (en) * | 2018-12-10 | 2021-09-14 | 北京工业大学 | Method for rapidly domesticating microorganism capable of degrading 2,4, 6-trichlorophenol |
| CN110606562A (en) * | 2019-09-23 | 2019-12-24 | 苏州科技大学 | Method for producing methane from high-concentration phenol-containing waste water |
| CN116396898A (en) * | 2023-03-10 | 2023-07-07 | 江苏诚冉环境修复工程有限公司 | 1, 2-trichloroethane degrading bacterium and application thereof |
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