CN106746436A - A kind of method of raising L glucose anaerobic degradations - Google Patents
A kind of method of raising L glucose anaerobic degradations Download PDFInfo
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- CN106746436A CN106746436A CN201710065049.9A CN201710065049A CN106746436A CN 106746436 A CN106746436 A CN 106746436A CN 201710065049 A CN201710065049 A CN 201710065049A CN 106746436 A CN106746436 A CN 106746436A
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- Prior art keywords
- glucose
- cys
- degradation rate
- raising
- temperature
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims abstract description 10
- 238000000034 method Methods 0.000 title claims abstract description 10
- 239000008103 glucose Substances 0.000 title claims abstract description 9
- 230000015556 catabolic process Effects 0.000 title abstract description 38
- 238000006731 degradation reaction Methods 0.000 title abstract description 38
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 239000010802 sludge Substances 0.000 claims abstract description 13
- 150000001413 amino acids Chemical class 0.000 claims abstract description 7
- 230000004151 fermentation Effects 0.000 claims abstract description 6
- 239000010865 sewage Substances 0.000 claims abstract description 3
- 238000003756 stirring Methods 0.000 claims abstract description 3
- GZCGUPFRVQAUEE-VANKVMQKSA-N aldehydo-L-glucose Chemical compound OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)C=O GZCGUPFRVQAUEE-VANKVMQKSA-N 0.000 claims description 45
- 230000002906 microbiologic effect Effects 0.000 claims description 2
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 description 8
- 239000005416 organic matter Substances 0.000 description 3
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical group Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 125000002353 D-glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 241000658379 Manihot esculenta subsp. esculenta Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000004088 foaming agent Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000004153 glucose metabolism Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000004890 malting Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000013615 non-nutritive sweetener Nutrition 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention belongs to environmental protection technical field, and in particular to a kind of method of raising L glucose anaerobic fermentation degraded.Sewage plant sludge and L glucose are added in reactor, sludge concentration（VSS）It is 1,000 2000 mg/L, by microbial acclimation, adds amino acid to carry out anaerobic fermentation, stir, control the pH value of reactor for 67, at a temperature of 25 37 DEG C, fermentation 48 days can significantly improves the efficiency of L glucose anaerobic degradations.
Description
Technical field
The invention belongs to environmental protection technical field, and in particular to a kind of method of raising L- glucose anaerobic degradations.
Background technology
Carbohydrate is the basic composition of most of organic wastes.For example, the content of polysaccharose substance is accounted in municipal sludge
20-40% of volatile organic matter total amount or so；In malting effluent such as cassava grain stillage, the content of polysaccharide accounts for the 30% of dissolubility COD
More than.Polysaccharose substance is monose, and then is converted into volatile fat in anaerobic digestion process by dissolving, hydrolysis
Acid and methane.Research shows there are two kinds of configurations in the monose of some polysaccharide hydrolysis generation；And the organic matter of various configuration is being detested
Different fermentation characters are shown during aerobe fermentation.By taking glucose as an example, there is D types glucose and L-type glucose.Natural bar
Under part, what nature was widely present is D-Glucose, and such material is easily utilized by microorganism etc. as carbon source, energy absorption.L-
Glucose is progressively applied in food industry, such as low-calorie sweeteners, foaming agent and antistaling agent.With chemical synthesis industry
Development and L- glucose extensive uses, increasing L- glucose will appear from the environment, but L- glucose is more difficult by ring
Border microbial degradation.Therefore, how research improves the biodegradable significant of L- glucose.
The content of the invention
It is an object of the invention to provide a kind of method of raising L- glucose anaerobic degradations.
The present invention is by the condition of optimizing the environment and adds the material with promotion bioactivity, accelerates the anaerobism of L- glucose
Metabolism, realizes the efficient anaerobic degraded of L- glucose.
The method of raising L- glucose anaerobic fermentation degraded proposed by the present invention, comprises the following steps that：
Sewage plant sludge and L- glucose are added in reactor, sludge concentration（TSS）It is 1000-2000 mg/L, by micro-
Biological domestication, adds amino acid to carry out anaerobic fermentation, stirs, and controls the pH value of reactor for 6-7, at a temperature of 25-37 DEG C, hair
Ferment 4-8 days, makes L- glucose fermentations degrade；Cys dosage is：The mass concentration of amino acid and the matter of L- glucose
Amount concentration ratio is 1:25-1:5.
In the present invention, described amino acid is Cys.
In the present invention, the condition of anaerobic fermentation is：Cys dosage is 1 with the mass concentration ratio of L- glucose:
10th, pH controls are in 37 DEG C of 6, temperature, 5 days time.
The beneficial effects of the invention are as follows：
（1）The degradation efficiency of L- glucose is improve, compared with similarity condition without Cys under preferable implementation condition
Control group degradation rate is increased substantially.
（2）Operating condition of the present invention is simply easily implemented, and effectively improves the conversion of L- glucose.
（3）The present invention improves the acidizing product of L- glucose, improves the resource efficiency of organic matter.
Specific embodiment
It is described in further detail with reference to example, it should be understood that the example lifted below is only for purposes of illustrating this
Invention, not including all the elements of the invention.
Embodiment 1
After by municipal sludge sieving removal bulk impurity, stand 24 h and be concentrated into the g/L of VSS 12 (TSS ≈ 17 g/L, pH ≈
7.0）, taking as original seed sludge after above-mentioned sludge distilled water cleans 3 times repeatedly, the concentration of L- glucose is 100 mg/L
Under conditions of the domestication sludge of 4 weeks or so as acclimation sludge.The concentration of substrate L- glucose is 500 mg/L, Cys
Add concentration for 50 mg/L, add a certain amount of acclimation sludge to make the VSS of anaerobic reaction mixed system be 1000 mg/L, adjust
Section pH is 6.0,37 DEG C of temperature, the d of anaerobic fermentation 5.The degradation rate of L- glucose is 100%, the mg/L/d of degradation rate 94.2.It is
1.6 times without the blank group of Cys and 1.5 times.
Embodiment 2
The concentration of substrate L- glucose is 500 mg/L, adds the mg/L of Cys 50, adjusts pH 5,37 DEG C of temperature, anaerobism
Ferment 5 d, and other operations are with embodiment 1.The degradation rate of gained L- glucose is 47.3%, and degradation rate is 46.6 mg/L/d,
The degradation rate and degradation rate of gained L- glucose improve 44% and 46% relative to blank group.
Embodiment 3
The concentration of substrate L- glucose is 500 mg/L, adds the mg/L of Cys 50, adjusts pH 7,37 DEG C of temperature, anaerobism
Ferment 5 d, and other operations are with embodiment 1.The degradation rate 74.1% of gained L- glucose, degradation rate is 71.5 mg/L/d, phase
For the blank group for not adding Cys, the degradation rate and degradation rate of gained L- glucose improve 1.8 times and 1.9
Times.
Embodiment 4
The concentration of substrate L- glucose is 500 mg/L, adds the mg/L of Cys 50, adjusts pH 8,37 DEG C of temperature, anaerobism
Ferment 5 d, and other operations are with embodiment 1.The degradation rate 27.8% of gained L- glucose, degradation rate is 26.1 mg/L/d, phase
For the blank group for not adding Cys, the degradation rate and degradation rate of gained L- glucose are without significant difference.
Embodiment 5
The concentration of substrate L- glucose is 500 mg/L, adds the mg/L of Cys 50, adjusts pH 9,37 DEG C of temperature, anaerobism
Ferment 5 d, and other operations are with embodiment 1.The degradation rate 17.2% of gained L- glucose, degradation rate is 16.2 mg/L/d, phase
For the blank group for not adding Cys, the degradation rate and degradation rate of gained L- glucose are without significant difference.
Embodiment 6
The concentration of substrate L- glucose is 500 mg/L, adds the mg/L of Cys 20, adjusts pH 6,37 DEG C of temperature, anaerobism
Ferment 5 d, and other operations are with embodiment 1.The degradation rate 85.0% of gained L- glucose, degradation rate is 85.4 mg/L/d, phase
For the blank group for not adding Cys, the degradation rate and degradation rate of gained L- glucose improve 40% and 50%.
Embodiment 7
The concentration of substrate L- glucose is 500 mg/L, adds the mg/L of Cys 100, adjusts pH 6, and 37 DEG C of temperature is detested
The d of aerobe fermentation 5, other operations are with embodiment 1.The degradation rate 100% of gained L- glucose, degradation rate is 99.8 mg/L/d, phase
For the blank group for not adding Cys, the degradation rate and degradation rate of gained L- glucose improve 64% and 71%.
Embodiment 8
The concentration of substrate L- glucose is 500 mg/L, adds the mg/L of Cys 50, adjusts pH 6,25 DEG C of temperature, anaerobism
Ferment 5 d, and other operations are with embodiment 1.The degradation rate 79.3% of gained L- glucose, degradation rate is 78.7 mg/L/d, phase
For the blank group for not adding Cys, the degradation rate and degradation rate of gained L- glucose improve 30% and 27%.
The above-mentioned description to embodiment is to be understood that and apply this hair for the ease of those skilled in the art
It is bright.Person skilled in the art obviously easily can make various modifications to these embodiments, and described herein
General Principle is applied in other embodiment without by performing creative labour.Therefore, the invention is not restricted to implementation here
Example, those skilled in the art's announcement of the invention, the improvement made for the present invention and modification all should be of the invention
Within protection domain.
Claims (3)
1. a kind of method that raising L- glucose anaerobic fermentation is degraded, it is characterised in that comprise the following steps that：
Sewage plant sludge and L- glucose are added in reactor, sludge concentration（VSS）It is 1000-2000 mg/L, by micro-
Biological domestication, adds amino acid to carry out anaerobic fermentation, stirs, and controls the pH value of reactor for 6-7, at a temperature of 25-37 DEG C, hair
Ferment 4-8 days, makes L- glucose fermentations degrade；Cys dosage is：The mass concentration of amino acid and the matter of L- glucose
Amount concentration ratio is 1:25-1:5.
2. method according to claim 1, it is characterised in that：Described amino acid is Cys.
3. method according to claim 1, it is characterised in that the condition of anaerobic fermentation is：Cys dosage and L-
The mass concentration ratio of glucose is 1:10th, pH controls are in 37 DEG C of 6, temperature, 5 days time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710065049.9A CN106746436B (en) | 2017-02-06 | 2017-02-06 | Method for improving anaerobic degradation of L-glucose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710065049.9A CN106746436B (en) | 2017-02-06 | 2017-02-06 | Method for improving anaerobic degradation of L-glucose |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106746436A true CN106746436A (en) | 2017-05-31 |
| CN106746436B CN106746436B (en) | 2020-06-26 |
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| CN201710065049.9A Expired - Fee Related CN106746436B (en) | 2017-02-06 | 2017-02-06 | Method for improving anaerobic degradation of L-glucose |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1827765A (en) * | 2006-01-23 | 2006-09-06 | 同济大学 | Screening method for anaerobe capable of degrading chlorophenol |
| US7232669B1 (en) * | 2006-03-10 | 2007-06-19 | Feng Chia University | Process for enhancing anaerobic biohydrogen production |
| CN101988075A (en) * | 2010-12-14 | 2011-03-23 | 东南大学 | Method for preparing hydrogen by fermentation through using special anaerobic clostridium pasteurianum |
| CN104478178A (en) * | 2014-12-05 | 2015-04-01 | 哈尔滨工业大学 | Microbial electrolysis two-section type sludge anaerobic digestion device and method for producing methane by using microbial electrolysis two-section type sludge anaerobic digestion device |
| CN104673712A (en) * | 2015-01-16 | 2015-06-03 | 山东省科学院能源研究所 | Bacterial strain for producing alcohol fuels by synchronously utilizing glucose and xylose and application of bacterial strain |
-
2017
- 2017-02-06 CN CN201710065049.9A patent/CN106746436B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1827765A (en) * | 2006-01-23 | 2006-09-06 | 同济大学 | Screening method for anaerobe capable of degrading chlorophenol |
| US7232669B1 (en) * | 2006-03-10 | 2007-06-19 | Feng Chia University | Process for enhancing anaerobic biohydrogen production |
| CN101988075A (en) * | 2010-12-14 | 2011-03-23 | 东南大学 | Method for preparing hydrogen by fermentation through using special anaerobic clostridium pasteurianum |
| CN104478178A (en) * | 2014-12-05 | 2015-04-01 | 哈尔滨工业大学 | Microbial electrolysis two-section type sludge anaerobic digestion device and method for producing methane by using microbial electrolysis two-section type sludge anaerobic digestion device |
| CN104673712A (en) * | 2015-01-16 | 2015-06-03 | 山东省科学院能源研究所 | Bacterial strain for producing alcohol fuels by synchronously utilizing glucose and xylose and application of bacterial strain |
Non-Patent Citations (2)
| Title |
|---|
| 王淑静等: "不同预处理温度对厌氧颗粒污泥发酵产氢的影响", 《化工学报》 * |
| 王继华等: "一种培养产氢发酵细菌的改良培养基", 《哈尔滨师范大学自然科学学报》 * |
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| CN106746436B (en) | 2020-06-26 |
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