CN106746436B - Method for improving anaerobic degradation of L-glucose - Google Patents
Method for improving anaerobic degradation of L-glucose Download PDFInfo
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- CN106746436B CN106746436B CN201710065049.9A CN201710065049A CN106746436B CN 106746436 B CN106746436 B CN 106746436B CN 201710065049 A CN201710065049 A CN 201710065049A CN 106746436 B CN106746436 B CN 106746436B
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- glucose
- cysteine
- degradation rate
- anaerobic fermentation
- degradation
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- WQZGKKKJIJFFOK-ZZWDRFIYSA-N L-glucose Chemical compound OC[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@H]1O WQZGKKKJIJFFOK-ZZWDRFIYSA-N 0.000 title claims abstract description 53
- 230000015556 catabolic process Effects 0.000 title claims abstract description 40
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 10
- 238000000855 fermentation Methods 0.000 claims abstract description 18
- 239000010802 sludge Substances 0.000 claims abstract description 15
- 239000010865 sewage Substances 0.000 claims abstract description 3
- 238000003756 stirring Methods 0.000 claims abstract description 3
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 34
- 235000013878 L-cysteine Nutrition 0.000 claims description 17
- 239000004201 L-cysteine Substances 0.000 claims description 17
- 244000005700 microbiome Species 0.000 claims description 3
- 150000001413 amino acids Chemical class 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 description 8
- PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 description 6
- 150000004676 glycans Chemical class 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 150000002772 monosaccharides Chemical class 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 241000658379 Manihot esculenta subsp. esculenta Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000004088 foaming agent Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000013615 non-nutritive sweetener Nutrition 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000012855 volatile organic compound Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention belongs to the technical field of environmental protection, and particularly relates to a method for improving anaerobic fermentation degradation of L-glucose. Adding sludge of a sewage plant and L-glucose into a reactor, wherein the sludge concentration (VSS) is 2000 mg/L and the sludge concentration (VSS) is 1000-2000 mg/L, performing microbial acclimation, adding amino acid for anaerobic fermentation, stirring, controlling the pH value of the reactor to be 6-7, and fermenting for 4-8 days at the temperature of 25-37 ℃, so that the efficiency of anaerobic degradation of the L-glucose can be obviously improved.
Description
Technical Field
The invention belongs to the technical field of environmental protection, and particularly relates to a method for improving anaerobic degradation of L-glucose.
Background
Sugars are the basic constituent of most organic wastes. For example, the content of polysaccharide substances in municipal sludge accounts for about 20-40% of the total amount of volatile organic compounds; in the brewing wastewater such as cassava vinasse, the content of polysaccharide accounts for more than 30 percent of the soluble COD. The polysaccharide substances are dissolved and hydrolyzed to be converted into monosaccharide in the anaerobic digestion process, and then volatile fatty acid and methane are generated through conversion. Studies have shown that there are two configurations in monosaccharides produced by hydrolysis of certain polysaccharides; while the organic matters with different configurations show different fermentation characteristics in the anaerobic fermentation process. In the case of glucose, there are D-glucose and L-glucose. Under natural conditions, D-glucose widely exists in the nature, and the substances are easily absorbed and utilized as carbon sources and energy sources by microorganisms and the like. L-glucose is increasingly used in the food industry, for example as low-calorie sweeteners, foaming agents, preservatives and the like. With the development of the chemical synthesis industry and the wide application of L-glucose, more and more L-glucose will appear in the environment, but L-glucose is difficult to be degraded by environmental microorganisms. Therefore, the research on how to improve the biodegradation of the L-glucose is of great significance.
Disclosure of Invention
The invention aims to provide a method for improving the anaerobic degradation of L-glucose.
The invention accelerates the anaerobic metabolism of the L-glucose by optimizing the environmental conditions and adding the substances with the biological activity promotion function, and realizes the high-efficiency anaerobic degradation of the L-glucose.
The invention provides a method for improving the anaerobic fermentation degradation of L-glucose, which comprises the following specific steps:
adding sludge of a sewage plant and L-glucose into a reactor, wherein the sludge concentration (TSS) is 1000-2000 mg/L, performing microbial acclimation, adding amino acid for anaerobic fermentation, stirring, controlling the pH value of the reactor to be 6-7, and fermenting at the temperature of 25-37 ℃ for 4-8 days to ferment and degrade the L-glucose; the dosage of the L-cysteine is as follows: the mass concentration ratio of the amino acid to the L-glucose is 1:25-1: 5.
In the invention, the amino acid is L-cysteine.
In the invention, the anaerobic fermentation conditions are as follows: the mass concentration ratio of the L-cysteine to the L-glucose is 1:10, the pH is controlled at 6, the temperature is 37 ℃, and the time is 5 days.
The invention has the beneficial effects that:
(1) the degradation efficiency of the L-glucose is improved, and the degradation rate of the L-glucose is greatly improved under better implementation conditions compared with that of a control group without adding L-cysteine under the same conditions.
(2) The method has simple and easy-to-implement operating conditions, and effectively improves the conversion of the L-glucose.
(3) The invention improves the acidification product of the L-glucose and the recycling efficiency of organic matters.
Detailed Description
The following examples are given to illustrate the present invention and not to limit the scope of the invention.
Example 1
Sieving municipal sludge to remove large impurities, standing for 24 h, concentrating to VSS 12 g/L (TSS is approximately equal to 17 g/L, pH is approximately equal to 7.0), repeatedly washing the sludge with distilled water for 3 times to be used as original inoculated sludge, and acclimating the sludge for about 4 weeks under the condition that the concentration of L-glucose is 100mg/L to be used as acclimated sludge. The concentration of substrate L-glucose is 500 mg/L, the adding concentration of L-cysteine is 50 mg/L, a certain amount of domesticated sludge is added to ensure that VSS of an anaerobic reaction mixed system is 1000 mg/L, the pH is adjusted to 6.0, the temperature is adjusted to 37 ℃, and anaerobic fermentation is carried out for 5 d. The degradation rate of the L-glucose is 100 percent, and the degradation rate is 94.2 mg/L/d. 1.6 times and 1.5 times the blank without addition of L-cysteine.
Example 2
The concentration of substrate L-glucose is 500 mg/L, 50 mg/L of L-cysteine is added, the pH is adjusted to be 5, the temperature is 37 ℃, anaerobic fermentation is carried out for 5 d, and other operations are the same as the example 1. The degradation rate of the obtained L-glucose is 47.3%, the degradation rate is 46.6 mg/L/d, and the degradation rate of the obtained L-glucose are improved by 44% and 46% relative to the blank group.
Example 3
The concentration of substrate L-glucose is 500 mg/L, 50 mg/L of L-cysteine is added, the pH is adjusted to be 7, the temperature is 37 ℃, anaerobic fermentation is carried out for 5 d, and other operations are the same as the example 1. The degradation rate of the obtained L-glucose is 74.1%, the degradation rate is 71.5 mg/L/d, and compared with a blank group without adding L-cysteine, the degradation rate and the degradation rate of the obtained L-glucose are improved by 1.8 times and 1.9 times.
Example 4
The concentration of substrate L-glucose is 500 mg/L, 50 mg/L of L-cysteine is added, the pH is adjusted to be 8, the temperature is 37 ℃, anaerobic fermentation is carried out for 5 d, and other operations are the same as the example 1. The degradation rate of the obtained L-glucose is 27.8%, the degradation rate is 26.1 mg/L/d, and compared with a blank group without adding L-cysteine, the degradation rate and the degradation rate of the obtained L-glucose have no obvious difference.
Example 5
The concentration of substrate L-glucose is 500 mg/L, 50 mg/L of L-cysteine is added, the pH is adjusted to 9, the temperature is 37 ℃, anaerobic fermentation is carried out for 5 d, and other operations are the same as example 1. The degradation rate of the obtained L-glucose is 17.2%, the degradation rate is 16.2 mg/L/d, and compared with a blank group without adding L-cysteine, the degradation rate and the degradation rate of the obtained L-glucose have no obvious difference.
Example 6
The concentration of substrate L-glucose is 500 mg/L, 20 mg/L of L-cysteine is added, the pH is adjusted to be 6, the temperature is 37 ℃, anaerobic fermentation is carried out for 5 d, and other operations are the same as the example 1. The degradation rate of the obtained L-glucose is 85.0%, the degradation rate is 85.4 mg/L/d, and compared with a blank group without adding L-cysteine, the degradation rate and the degradation rate of the obtained L-glucose are improved by 40% and 50%.
Example 7
The concentration of substrate L-glucose is 500 mg/L, 100mg/L of L-cysteine is added, the pH is adjusted to 6, the temperature is 37 ℃, anaerobic fermentation is carried out for 5 d, and other operations are the same as example 1. The degradation rate of the obtained L-glucose is 100%, the degradation rate is 99.8 mg/L/d, and compared with a blank group without adding L-cysteine, the degradation rate and the degradation rate of the obtained L-glucose are improved by 64% and 71%.
Example 8
The concentration of substrate L-glucose is 500 mg/L, 50 mg/L of L-cysteine is added, the pH is adjusted to be 6, the temperature is 25 ℃, anaerobic fermentation is carried out for 5 d, and other operations are the same as example 1. The degradation rate of the obtained L-glucose is 79.3%, the degradation rate is 78.7 mg/L/d, and compared with a blank group without adding L-cysteine, the degradation rate and the degradation rate of the obtained L-glucose are improved by 30% and 27%.
The embodiments described above are intended to facilitate the understanding and appreciation of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the embodiments described herein, and those skilled in the art should make improvements and modifications to the present invention based on the disclosure of the present invention within the protection scope of the present invention.
Claims (2)
1. A method for improving the anaerobic fermentation degradation of L-glucose is characterized by comprising the following specific steps:
adding sludge of a sewage plant and L-glucose into a reactor, wherein the sludge concentration (VSS) is 2000 mg/L and the sludge concentration (VSS) is 1000-2000 mg/L, acclimating by microorganisms, adding L-cysteine to perform anaerobic fermentation, stirring, controlling the pH value of the reactor to be 6-7, and fermenting for 4-8 days at the temperature of 25-37 ℃ to ferment and degrade the L-glucose; the dosage of the L-cysteine is as follows: the mass concentration ratio of the L-cysteine to the L-glucose is 1:25-1: 5.
2. The method according to claim 1, characterized in that the conditions of anaerobic fermentation are: the mass concentration ratio of the L-cysteine to the L-glucose is 1:10, the pH is controlled at 6, the temperature is 37 ℃, and the time is 5 days.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710065049.9A CN106746436B (en) | 2017-02-06 | 2017-02-06 | Method for improving anaerobic degradation of L-glucose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710065049.9A CN106746436B (en) | 2017-02-06 | 2017-02-06 | Method for improving anaerobic degradation of L-glucose |
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| Publication Number | Publication Date |
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| CN106746436A CN106746436A (en) | 2017-05-31 |
| CN106746436B true CN106746436B (en) | 2020-06-26 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1827765A (en) * | 2006-01-23 | 2006-09-06 | 同济大学 | Screening method for anaerobe capable of degrading chlorophenol |
| US7232669B1 (en) * | 2006-03-10 | 2007-06-19 | Feng Chia University | Process for enhancing anaerobic biohydrogen production |
| CN101988075A (en) * | 2010-12-14 | 2011-03-23 | 东南大学 | Method for preparing hydrogen by fermentation through using special anaerobic clostridium pasteurianum |
| CN104478178A (en) * | 2014-12-05 | 2015-04-01 | 哈尔滨工业大学 | Microbial electrolysis two-section type sludge anaerobic digestion device and method for producing methane by using microbial electrolysis two-section type sludge anaerobic digestion device |
| CN104673712A (en) * | 2015-01-16 | 2015-06-03 | 山东省科学院能源研究所 | Bacterial strain for producing alcohol fuels by synchronously utilizing glucose and xylose and application of bacterial strain |
-
2017
- 2017-02-06 CN CN201710065049.9A patent/CN106746436B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1827765A (en) * | 2006-01-23 | 2006-09-06 | 同济大学 | Screening method for anaerobe capable of degrading chlorophenol |
| US7232669B1 (en) * | 2006-03-10 | 2007-06-19 | Feng Chia University | Process for enhancing anaerobic biohydrogen production |
| CN101988075A (en) * | 2010-12-14 | 2011-03-23 | 东南大学 | Method for preparing hydrogen by fermentation through using special anaerobic clostridium pasteurianum |
| CN104478178A (en) * | 2014-12-05 | 2015-04-01 | 哈尔滨工业大学 | Microbial electrolysis two-section type sludge anaerobic digestion device and method for producing methane by using microbial electrolysis two-section type sludge anaerobic digestion device |
| CN104673712A (en) * | 2015-01-16 | 2015-06-03 | 山东省科学院能源研究所 | Bacterial strain for producing alcohol fuels by synchronously utilizing glucose and xylose and application of bacterial strain |
Non-Patent Citations (2)
| Title |
|---|
| 一种培养产氢发酵细菌的改良培养基;王继华等;《哈尔滨师范大学自然科学学报》;20061231(第06期);第86页右栏第1段至第88页最后1段 * |
| 不同预处理温度对厌氧颗粒污泥发酵产氢的影响;王淑静等;《化工学报》;20110331;第62卷(第3期);第812页左栏第2段至第814页左栏最后1段 * |
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| CN106746436A (en) | 2017-05-31 |
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