CN1792483A - Process of botany for repairing soil composite polluted by polycyclic aromatic hydrocarbon - Google Patents
Process of botany for repairing soil composite polluted by polycyclic aromatic hydrocarbon Download PDFInfo
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- CN1792483A CN1792483A CN 200610037639 CN200610037639A CN1792483A CN 1792483 A CN1792483 A CN 1792483A CN 200610037639 CN200610037639 CN 200610037639 CN 200610037639 A CN200610037639 A CN 200610037639A CN 1792483 A CN1792483 A CN 1792483A
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- aromatic hydrocarbon
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Abstract
A phytologic method for repairing the soil polluted by polycycloarylhydrocarbon features that the leguminous plant such as alfalfa, trifolium, etc is planted in said polluted soil and it is harvested after it is growing for 85-95 days.
Description
Technical field
The present invention relates to a kind of biology green technology, be specifically related to a kind of method of subduing polycyclic aromatic hydrocarbon combined pollutant in the soil: the botany method that is used to repair the polycyclic aromatic hydrocarbon combined contamination soil.
Background technology
(the Polycyclic Aromatic Hydrocarbons of polycyclic aromatic hydrocarbon in the environment, PAHs) extensively exist, as media such as soil, mud, water body, oil, it is the persistence organic pollutant of a quasi-representative, have higher carcinogenic, teratogenesis, mutagenicity, ecological environment security and human health are caused bigger threat.Therefore, in the soil control of persistence organic pollutant such as polycyclic aromatic hydrocarbon with repair the important research content that has become home and abroad environment Science and Technology field.The restorative procedure of relevant contaminated soil, mainly contain chemistry, method such as physics, microorganism and phytoremediation, wherein phytoremediation is the important biomolecule technology of at present tool development prospect.Phytoremediation is to utilize plant in growth course, a kind of in-situ treatment technology of plant absorbing, degraded, stable, volatilization and root circle renovation of organic pollution soil.It not only has ecoaesthetics value, and is cheap, and can make full use of natural regenerated resources solar energy, subdues organic pollution in the soil, low contaminated soil in being suitable for.Therefore, obtain plant germplasm resource and become this technology key link with reparation polycyclic aromatic hydrocarbon combined contamination soil.Leguminous herbaceous plant's biomass is big, nutritious, not only can be used as good feed, and strong stress resistance, well developed root system, coring is powerful, can secrete a large amount of root exudates, simultaneously lateral root and is given birth to a lot of root nodules, and the nitrogen in the fixed air can be that microorganism provides sufficient nitrogen nutrition in the soil.Does this root circle function help promoting the degraded of multiple polycyclic aromatic hydrocarbon in the soil? usually can repeatedly cradle every year, whether its aerial part also can directly take away organic pollution, waits repair function, knows little about it at present.Therefore, this research is research object with Yangtze River Delta somewhere PAHs combined contamination soil, 3 kinds of legumes such as plantation alfalfa, clover and sweet potato etc. PAHs combined pollutant in the soil of degrading is for the phytoremediation technology of research and development PAHs combined contamination soil provides basic material.
Summary of the invention
The present invention will provide a kind of soil for combined pollution to have the biological method of good repairing effect, promptly, adopt plantation legume alfalfa, clover and sweet potato to reduce polycyclic aromatic hydrocarbon combined pollutant in the long-term pollution soil, for the phytoremediation technology of researching and developing the PAHs combined contamination soil provides basic material.
The scheme of finishing the foregoing invention task is: be used to repair the botany method of polycyclic aromatic hydrocarbon combined contamination soil, may further comprise the steps:
On the polycyclic aromatic hydrocarbon combined contamination soil, plant legume, grow to 85～95 days and cradle.
Above-described legume can be the various legumes of planting as crops, green manure and environmental protection at present.For example clover, clover and sweet potato etc.
Described " plantation " comprises seeding method of the prior art and planting patterns, and wherein seeding method comprises direct seeding technique and culturing and transplanting seedlings in the soil, and planting patterns adopts nonoculture, crop rotation and intercropping method.
Described combined pollutant is polycyclic aromatic hydrocarbon persistence organic pollutants such as low ring and epipodium.For example: low lopps has naphthalene, anthracene, fluorenes, acenaphthene, phenanthrene etc.; Epipodium pyrene, fluoranthene, benzo [a] anthracene, benzo [b] fluoranthene, benzo (a) pyrene etc. are arranged
The further optimization of the above technical scheme, scheme is as follows:
1, described legume is adopted perennial leguminous herbaceous plant, and more the scheme of You Huaing is to adopt alfalfa (Medicago sativa L.);
2, described planting patterns adopts live and nonoculture;
3, before planting, increase following steps:
Seed Treatment: selecting full, hard seed rate is about 10% alfalfa seed, and short-term high-temperature process (50 ℃, 15 minutes) before broadcasting improves germination percentage.Use the dipped into formalin alfalfa seed of 50 times of dilutions again, disinfect, can prevent the generation of clover ring spot.
The present invention fully excavates the potentiality that legume is repaired the polycyclic aromatic hydrocarbon combined pollutant, utilizes conversion, absorption and the root circle repair mechanisms of leguminous forage, removes polycyclic aromatic hydrocarbon in the soil and a kind of plant restoration method of designing.This method is selected legume alfalfa, clover and sweet potato for use, is applied to the polycyclic aromatic hydrocarbon combined contamination soil, improving plant roots district micro-ecological environment, has obviously improved the degraded of polycyclic aromatic hydrocarbon in the soil or has subdued effect.
Result of study shows that the content of roots of alfalfa, stem, China and Philippines of leaf texture (PA) is 39.8 μ g kg
-1, 294.8 μ g kg
-1, 203.0 μ g kg
-1, and the content of benzo [a] pyrene (B[a] P) is respectively 2.1 μ g kg
-1, 0.7 μ g kg
-1, 0.8 μ g kg
-1The content of clover root, stem, China and Philippines of leaf texture (PA) is 26.4gkg
-1, 156.4 μ g kg
-1, 162.1 μ g kg
-1, and the content of benzo [a] pyrene (B[a] P) is respectively 1.3 μ g kg
-1, 0.4 μ g kg
-1, 0.5 μ g kg
-1The content of sweet potato root, stem, China and Philippines of leaf texture (PA) is 12.7 μ g kg
-1, 68.3 μ g kg
-1, 54.4 μ g kg
-1, and the content of benzo [a] pyrene (B[a] P) is respectively 0.5 μ g kg
-1, 0.3 μ g kg
-1, 0.2 μ g kg
-1Compare with control group (not planting plant), behind plantation alfalfa, clover, the sweet potato, degradation rate for examination soil China and Philippines (PA) and benzo [a] pyrene (B[a] P) has obtained raising in various degree, its degradation rate scope is respectively 25.2%～54.3%, 7.7%～21.5%, 2.8%～8.7%, it is the highest wherein to plant alfalfa.This result shows conversion, absorption and the root circle repair mechanisms of utilizing the legume alfalfa, is applied to the PAHs combined contamination soil, be a kind of comparatively ideal, cheaply, the plant restoration method of the friendly type of environmental protection.
The specific embodiment
Embodiment 1:
For trying soil: pick up from Yangtze River Delta somewhere polycyclic aromatic hydrocarbon pollution, natural air drying is crossed the 3mm sieve, and regulating soil moisture content is 55% of field capacity, to be for experiment.Before the reparation, for polycyclic aromatic hydrocarbon phenanthrene (PA) and benzo p[a in the examination soil] pyrene (B[a] P) is respectively 50.5 μ g/kg, 12.7 μ g/kg.
Test plant: alfalfa (Medicago sativa L.), be the pulse family herbaceos perennial, be most important in the world tame forage grass, be called as " king of herbage ".
Testing program: 2 processing of this research and design, i.e. contrast (handling 1) and plantation alfalfa (handling 2).Repeat 4 times, district's group is arranged at random.
Test method: a certain amount of base manure is imposed in polycyclic aromatic hydrocarbon (PAHs) combined contamination soil, fully mix thoroughly.And in time the alfalfa seed of handling well is seeded in the basin alms bowl, earthing places the constant temperature and humidity illumination cultivation indoor.Carry out final singling, Routine Management after emerging.Post-sampling analysis in 90 days is carried out in test.
Result of study shows that the content of roots of alfalfa, stem, China and Philippines of leaf texture (PA) is 39.8 μ g kg
-1, 294.8 μ g kg
-1, 203.0 μ g kg
-1, and the content of benzo [a] pyrene (B[a] P) is respectively 2.1 μ g kg
-1, 0.7 μ g kg
-1, 0.8 μ g kg
-1Compared with the control, after the plantation alfalfa, obviously improve for the degradation rate of polycyclic aromatic hydrocarbon (PAHs) in the examination soil, the degradation rate scope of soil China and Philippines (PA) and benzo [a] pyrene (B[a] P) reaches 25%～54%.This result shows conversion, absorption and the root circle repair mechanisms of utilizing the legume alfalfa, is applied to the PAHs combined contamination soil, be a kind of comparatively ideal, cheaply, the plant restoration method of the friendly type of environmental protection.
Embodiment 2, and is basic identical with embodiment one, but after used plant changed clover into, the content of clover root, stem, China and Philippines of leaf texture (PA) was 26.4g kg
-1, 156.4 μ g kg
-1, 162.1 μ g kg
-1, and the content of benzo [a] pyrene (B[a] P) is respectively 1.3 μ g kg
-1, 0.4 μ g kg
-1, 0.5 μ g kg
-1, be 7.7%～21.5% for the degradation rate scope of examination soil China and Philippines (PA) and benzo [a] pyrene (B[a] P).
Embodiment 3, and is basic identical with embodiment one, but after used plant changed sweet potato into, the content of sweet potato root, stem, China and Philippines of leaf texture (PA) was 12.7 μ g kg
-1, 68.3 μ g kg
-1, 54.4 μ g kg
-1, and the content of benzo [a] pyrene (B[a] P) is respectively 0.5 μ g kg
-1, 0.3 μ g kg
-1, 0.2 μ g kg
-1, reach 2.8%～8.7% for the degradation rate scope of examination soil China and Philippines (PA) and benzo [a] pyrene (B[a] P).
Claims (6)
1, a kind of botany method that is used to repair the polycyclic aromatic hydrocarbon combined contamination soil may further comprise the steps: plant legume on the polycyclic aromatic hydrocarbon combined contamination soil, grow to 85～95 days and cradle.
2, according to the described botany method that is used to repair the polycyclic aromatic hydrocarbon combined contamination soil of claim 1, it is characterized in that described legume is adopted perennial leguminous herbaceous plant.
According to the described botany method that is used to repair the polycyclic aromatic hydrocarbon combined contamination soil of claim 2, it is characterized in that 3, described perennial leguminous herbaceous plant adopts alfalfa, grows to 90 days and cradles.
4, the botany method of stating according to claim 1 or 2 or 3 that is used to repair the polycyclic aromatic hydrocarbon combined contamination soil, it is characterized in that the combined pollutant in the described polycyclic aromatic hydrocarbon combined contamination soil is the polycyclic aromatic hydrocarbon persistence organic pollutant: luxuriant and rich with fragrance and benzo [a] pyrene.
5, according to the described botany method that is used to repair the polycyclic aromatic hydrocarbon combined contamination soil of claim 4, it is characterized in that, before planting, increase following steps:
Seed Treatment: selecting full, hard seed rate is about 10% alfalfa seed, and short-term high-temperature process before broadcasting improves germination percentage, uses the dipped into formalin alfalfa seed of 50 times of dilutions again, disinfects.
According to the described botany method that is used to repair the polycyclic aromatic hydrocarbon combined contamination soil of claim 5, it is characterized in that 6, the condition of short-term high-temperature process was before described seed was broadcast: 50 ℃, 15 minutes.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101844147A (en) * | 2010-05-24 | 2010-09-29 | 延安市微生物研究所 | Method for using leguminous plants for restoring petroleum-polluted soil |
| CN102553899A (en) * | 2010-12-09 | 2012-07-11 | 中国科学院沈阳应用生态研究所 | Method for remediating polycyclic aromatic hydrocarbons (PAHs) contaminated soil by utilizing flowering plant Echinacea purpurea |
| CN102784798A (en) * | 2012-08-14 | 2012-11-21 | 大连民族学院 | Method for biologically remedying coil contaminated by coking wastewater |
| CN105478461A (en) * | 2016-01-13 | 2016-04-13 | 三峡大学 | Method for repairing soil seriously polluted by polycyclic aromatic hydrocarbon by utilizing plants |
| CN105665438A (en) * | 2016-01-13 | 2016-06-15 | 三峡大学 | Method for repairing soil severely polluted by petroleum hydrocarbon through plants |
| CN106976875A (en) * | 2017-04-24 | 2017-07-25 | 福建师范大学 | A kind of method for reducing polycyclic aromatic hydrocarbon content in sludge base charcoal |
| CN108213064A (en) * | 2017-12-05 | 2018-06-29 | 中国科学院南京土壤研究所 | A kind of medicament of in-situ immobilization high concentration polycyclic aromatic hydrocarbon pollution and its application |
| CN108246785A (en) * | 2017-12-15 | 2018-07-06 | 昆明理工大学 | A kind of method for enhancing phytoremediation polycyclic aromatic hydrocarbon pollution efficiency |
| CN108372191A (en) * | 2018-02-02 | 2018-08-07 | 贵州大学 | A kind of method of " plant-enzyme " combination degradation PAHs in soil |
| CN108655161A (en) * | 2018-05-25 | 2018-10-16 | 四川川能环保科技有限公司 | A kind of biology in situ renovation method of organic material contaminated soil |
| CN108856284A (en) * | 2018-06-28 | 2018-11-23 | 西北工业大学 | Rhizobium-legume symbiosis system, building and the biodegrading process of degrading polycyclic aromatic hydrocarbons |
| CN109702001A (en) * | 2018-12-27 | 2019-05-03 | 钦州学院 | A method of utilizing soil nematodes rehabilitating soil fertility |
| CN111069274A (en) * | 2019-12-17 | 2020-04-28 | 中国科学院南京土壤研究所 | Nano-bioremediation method for soil in high-concentration polycyclic aromatic hydrocarbon contaminated site |
| CN114101321A (en) * | 2021-12-08 | 2022-03-01 | 沈阳大学 | Method for absorbing and degrading tri (1-chloro-2-propyl) phosphate in soil by using vetch of leguminous plant |
-
2006
- 2006-01-06 CN CN 200610037639 patent/CN1792483A/en active Pending
Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101844147A (en) * | 2010-05-24 | 2010-09-29 | 延安市微生物研究所 | Method for using leguminous plants for restoring petroleum-polluted soil |
| CN101844147B (en) * | 2010-05-24 | 2011-09-28 | 延安市微生物研究所 | Method for using leguminous plants for restoring petroleum-polluted soil |
| CN102553899A (en) * | 2010-12-09 | 2012-07-11 | 中国科学院沈阳应用生态研究所 | Method for remediating polycyclic aromatic hydrocarbons (PAHs) contaminated soil by utilizing flowering plant Echinacea purpurea |
| CN102784798A (en) * | 2012-08-14 | 2012-11-21 | 大连民族学院 | Method for biologically remedying coil contaminated by coking wastewater |
| CN105478461A (en) * | 2016-01-13 | 2016-04-13 | 三峡大学 | Method for repairing soil seriously polluted by polycyclic aromatic hydrocarbon by utilizing plants |
| CN105665438A (en) * | 2016-01-13 | 2016-06-15 | 三峡大学 | Method for repairing soil severely polluted by petroleum hydrocarbon through plants |
| CN105665438B (en) * | 2016-01-13 | 2018-06-19 | 三峡大学 | A kind of method of the soil petroleum hydrocarbon contaminated using phytoremediation severe |
| CN105478461B (en) * | 2016-01-13 | 2018-06-19 | 三峡大学 | A kind of method using phytoremediation severe polycyclic aromatic hydrocarbon pollution |
| CN106976875A (en) * | 2017-04-24 | 2017-07-25 | 福建师范大学 | A kind of method for reducing polycyclic aromatic hydrocarbon content in sludge base charcoal |
| CN108213064A (en) * | 2017-12-05 | 2018-06-29 | 中国科学院南京土壤研究所 | A kind of medicament of in-situ immobilization high concentration polycyclic aromatic hydrocarbon pollution and its application |
| CN108246785A (en) * | 2017-12-15 | 2018-07-06 | 昆明理工大学 | A kind of method for enhancing phytoremediation polycyclic aromatic hydrocarbon pollution efficiency |
| CN108246785B (en) * | 2017-12-15 | 2020-12-15 | 昆明理工大学 | Method for enhancing efficiency of phytoremediation of polycyclic aromatic hydrocarbon-polluted soil |
| CN108372191A (en) * | 2018-02-02 | 2018-08-07 | 贵州大学 | A kind of method of " plant-enzyme " combination degradation PAHs in soil |
| CN108655161A (en) * | 2018-05-25 | 2018-10-16 | 四川川能环保科技有限公司 | A kind of biology in situ renovation method of organic material contaminated soil |
| CN108856284A (en) * | 2018-06-28 | 2018-11-23 | 西北工业大学 | Rhizobium-legume symbiosis system, building and the biodegrading process of degrading polycyclic aromatic hydrocarbons |
| CN109702001A (en) * | 2018-12-27 | 2019-05-03 | 钦州学院 | A method of utilizing soil nematodes rehabilitating soil fertility |
| CN109702001B (en) * | 2018-12-27 | 2020-12-29 | 钦州学院 | Method for restoring soil fertility by utilizing soil nematodes |
| CN111069274A (en) * | 2019-12-17 | 2020-04-28 | 中国科学院南京土壤研究所 | Nano-bioremediation method for soil in high-concentration polycyclic aromatic hydrocarbon contaminated site |
| CN111069274B (en) * | 2019-12-17 | 2021-08-10 | 中国科学院南京土壤研究所 | Nano-bioremediation method for soil in high-concentration polycyclic aromatic hydrocarbon contaminated site |
| US11278944B2 (en) * | 2019-12-17 | 2022-03-22 | Institute Of Soil Science, Chinese Academy Of Sciences | Nanobioremediation method for soil in high-concentration polycyclic aromatic hydrocarbon (PAH)-contaminated site |
| CN114101321A (en) * | 2021-12-08 | 2022-03-01 | 沈阳大学 | Method for absorbing and degrading tri (1-chloro-2-propyl) phosphate in soil by using vetch of leguminous plant |
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