CN1778163A

CN1778163A - Cultivation of cryophilous plant hybrid species except rape by high-temperature sterile line

Info

Publication number: CN1778163A
Application number: CN 200410052297
Authority: CN
Inventors: 何觉民; 陆建农; 何仪; 王丰青
Original assignee: Zhanjiang Marine University
Current assignee: Zhanjiang Marine University
Priority date: 2004-11-22
Filing date: 2004-11-22
Publication date: 2006-05-31

Abstract

A method for culturing the cryophilous hybridized plants except rape by use of high-temp sterile line features that the sterile line whose male development is benefit from the lowering of temp is used.

Description

Utilize the cool plant hybrid of the happiness of high temperature male sterile line production except that rape

Technical field.The invention belongs to field of plant breeding.A method of producing plant hybrid specifically.

Background technology.At present, the method that people produce the cool plant hybrid of happiness except that rape is that 1. the chemical hybridizing agent method promptly utilizes chemical agent to stop pollen development or the anther dehiscence that is used as maternal kind, make it male sterile, thereby produce the cool plant hybrid of happiness with the different intergrowth of male parent; 2. three line method is promptly used the different friendship breeding male sterile lines of maintenance line and male sterile line seed, with recovering system and the different friendship of male sterile line, produces crossbreed; 3. low temperature male sterile line method, i.e. self propagated low temperature male sterile line under condition more than 13 ℃ or 18 ℃ is that different intergrowth produces crossbreed with recovery under 13 ℃ or 18 ℃ of following conditions.Yet chemical hybridizing agent is difficult to when dispenser evenly, make maternal sterile not thoroughly and selfing and produce maternal seed influence hybrid purity and output, be difficult to be applied to production.Three line method is then because during the different friendship breeding of male sterile line and maintenance line, and maintenance line is very easily sneaked in the male sterile line and caused and mix, thereby produces a large amount of sterile strains and can not be used for production in hybrid.Low temperature male sterile line method be utilize in performance below 13 ℃ or 18 ℃ sterile, and the male sterile line production of hybrid seeds under cryogenic conditions that can educate in performance more than 13 ℃ or 18 ℃, and under hot conditions the method for breeding male sterile lines.Because China likes cool plant main producing region---the north cool flowering of plant of happiness is later, the anther development stage is in temperature higher season, the low temperature male sterile line in the north general performance can educate, therefore the low temperature male sterile line generally can only be bred in the north, if use the low temperature sterile lines seed making in the north, then be easy to self-fertility, influence hybrid purity.The cool flowering of plant of China's south happiness is morning, and the anther development phase temperature is lower, though be the sterile district of low temperature male sterile line, because low temperature is followed wet weather simultaneously, outcrossing seed-setting rate is low in the production of hybrid seeds process, and the hybrid germination rate is low.Low temperature male sterile line method also is difficult to large-area applications in producing (He Juemin etc., two-line-method hybrid wheat theory and practice, Changsha, Hunan science tech publishing house, 1993; He Beiru, Dong Puhui, Song Xiyue, etc. the research of wheat responsive to temperature male sterile line A3314 temperature-sensing property, wheat crops journal, 2003,23 (1): 1～6).

Summary of the invention.Purpose of the present invention is to create a kind of method that can like the cool plant hybrid of the happiness of cool plant main producing region-northern large tracts of land production except that rape in China.Particular content of the present invention is, serviceability temperature raises and is unfavorable for its patrogenesis, temperature reduces the cool plant hybrid of the happiness of male sterile line production except that rape that helps its patrogenesis, so that the cool plant male sterile line of the happiness of breeding except that rape under the condition of happiness cool plant anther developmental stage temperature lower (day samming is below 13 ℃), under the condition of happiness cool plant anther developmental stage temperature higher (day samming more than 13 ℃) with the different friendship of male parent, produce the cool plant hybrid of happiness except that rape, or the southern self propagated male sterile line of low temperature often there is in happiness the cool plant pollen developmental stage, and, produce crossbreed in the higher north and the different friendship of male parent of happiness cool plant pollen developmental stage temperature; Or under southern autumn sowing (broadcasting before October 25) condition breeding male sterile lines, and under southern winter sowing (broadcasting after October 25) condition, be different friendship with recovery, produce the happiness cold plant hybrid except that rape; Or the autumn and winter broadcast the happiness cold plant male sterile line of breeding except that rape, spring and summer is broadcast and the different friendship of male parent, produce the cool plant hybrid of happiness except that rape, or broadcast the happiness cold plant male sterile line of breeding except that rape in low height above sea level (below 500 meters) low latitudes (32 ° on the south the N) the area autumn and winter, broadcast and the different friendship of male parent in high latitude (40 ° to the north of the N) or high altitude localities (more than 1800 meters) spring and summer, produce the cool plant hybrid of happiness except that rape.

Generally speaking, the sterile temperature of the cool plant male sterile line of the sterile happiness of the high temperature except that rape is below 13 ℃ more than 13 ℃ and can educate temperature.

The present invention can make the cool plant hybrid of the happiness beyond the oil removing dish become a reality.

The sterile wheat of embodiment 1 high temperature week wheat 13s-1 can educate or half sterile 32 ° of autumn sowings on the south the N, and the self propagated seed is sterile 32 ° of autumn sowings to the north of the N, with No. 21 different friendships of middle wheat, produces the crossbreed of all wheat 13s-1 and middle wheat No. 21.

Embodiment 2 broad bean kinds open No. 3, beans and move northward Hebei by Jiangsu and sow in spring, and Yin Gaowen causes sterile, with the different friendship of high temperature resistant strain 04-1, produce and open No. 3, beans and 04-1 crossbreed.

The precocious No. 3 Henan autumn sowings of embodiment 3 barley varieties, sterile strain appears under hot conditions, force selfing to obtain sterile strain selfed seed, sterile strain selfed seed in the Hunan autumn sowing, can be educated under cryogenic conditions or half sterile, and selfing is isozygotied, the precocious No. 3 s selfed seeds of Hunan autumn sowing breeding, the Henan autumn sowing is sterile, with the different friendship of R24, produces the crossbreed of precocious No. 3 s and R24.

Claims

1. utilize the cool plant hybrid of the happiness of high temperature male sterile line production except that rape, it is characterized in that, serviceability temperature raises and is unfavorable for its patrogenesis, and temperature reduces the cool plant hybrid of the happiness of male sterile line production except that rape that helps its patrogenesis.

2. method according to claim 1, it is characterized in that, the cool plant male sterile line of the happiness of breeding except that rape under the condition of happiness cool plant pollen developmental stage temperature lower (day samming is below 13 ℃), under the condition of happiness cool plant pollen developmental stage temperature higher (day samming is more than 13 ℃),, produce the cool plant male sterile line of the happiness crossbreed except that rape with the different friendship of male parent.

3. method according to claim 1 is characterized in that, the autumn and winter are broadcast the cool plant male sterile line of the happiness of breeding except that rape, and spring and summer is broadcast and the different friendship of male parent, produces the cool plant hybrid of happiness except that rape.

4. method according to claim 1, it is characterized in that, the cool plant male sterile line of the happiness of southern self propagated except that rape that low temperature was often arranged in the happiness cool plant pollen developmental stage, and, produce the cool plant hybrid of happiness except that rape in the higher north and the different friendship of male parent of happiness cool plant pollen developmental stage temperature.

5. method according to claim 1, it is characterized in that, the male sterile line of breeding except that rape under southern autumn sowing (broadcasting before October 25) condition, and under southern winter sowing (broadcasting after October 25) condition with the different friendship of male parent, the happiness cold plant hybrid of production except that rape.

6. method according to claim 1, it is characterized in that, broadcast the cool plant male sterile line of the happiness of breeding except that rape in low height above sea level (below 500 meters) low latitudes (32 ° on the south the N) the area autumn and winter, broadcast and the different friendship of male parent in high latitude (40 ° to the north of the N) or high altitude localities (more than 1800 meters) spring and summer, produce the cool plant hybrid of happiness except that rape.