CN1749282A - Belly bottom snail polysaccharide and its preparing method - Google Patents
Belly bottom snail polysaccharide and its preparing method Download PDFInfo
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- CN1749282A CN1749282A CN 200510047411 CN200510047411A CN1749282A CN 1749282 A CN1749282 A CN 1749282A CN 200510047411 CN200510047411 CN 200510047411 CN 200510047411 A CN200510047411 A CN 200510047411A CN 1749282 A CN1749282 A CN 1749282A
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- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 79
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- 241000237858 Gastropoda Species 0.000 title claims description 31
- 238000000034 method Methods 0.000 title description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 56
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 48
- 238000001556 precipitation Methods 0.000 claims abstract description 41
- 235000013372 meat Nutrition 0.000 claims abstract description 32
- 235000013305 food Nutrition 0.000 claims abstract description 16
- 239000000203 mixture Substances 0.000 claims abstract description 15
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 10
- 230000006870 function Effects 0.000 claims abstract description 8
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 8
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 8
- 230000001105 regulatory effect Effects 0.000 claims abstract description 6
- 102000004190 Enzymes Human genes 0.000 claims description 34
- 108090000790 Enzymes Proteins 0.000 claims description 34
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 20
- 230000000694 effects Effects 0.000 claims description 16
- 239000000284 extract Substances 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 16
- 102000011759 adducin Human genes 0.000 claims description 14
- 108010076723 adducin Proteins 0.000 claims description 14
- 239000012141 concentrate Substances 0.000 claims description 14
- 239000002994 raw material Substances 0.000 claims description 11
- AMQJEAYHLZJPGS-UHFFFAOYSA-N N-Pentanol Chemical compound CCCCCO AMQJEAYHLZJPGS-UHFFFAOYSA-N 0.000 claims description 10
- 238000010792 warming Methods 0.000 claims description 10
- 230000001093 anti-cancer Effects 0.000 claims description 8
- 230000036737 immune function Effects 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 8
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- 102000035092 Neutral proteases Human genes 0.000 claims description 7
- 108091005507 Neutral proteases Proteins 0.000 claims description 7
- 230000005764 inhibitory process Effects 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 7
- 210000002784 stomach Anatomy 0.000 claims description 7
- 238000012546 transfer Methods 0.000 claims description 7
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 4
- 238000000108 ultra-filtration Methods 0.000 claims description 4
- 238000002525 ultrasonication Methods 0.000 claims description 4
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 230000001681 protective effect Effects 0.000 claims description 3
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- 238000004108 freeze drying Methods 0.000 abstract description 3
- 235000013376 functional food Nutrition 0.000 abstract description 2
- 241001274658 Modulus modulus Species 0.000 abstract 5
- 201000011510 cancer Diseases 0.000 abstract 1
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- 241001465754 Metazoa Species 0.000 description 3
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- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 3
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- 235000016709 nutrition Nutrition 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
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- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- 235000019698 starch Nutrition 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to polysaccharide matter, and is belly button snail polysaccharide of purity 25-55 % and produced with belly button snail meat and/or leftover, and through homogenizing, enzymolysis, water extracting, concentration, alcohol precipitation, eliminating protein, alcohol precipitation and freeze drying. The present invention also provides food composition with the belly button snail polysaccharide as active component. The present invention combines ultrasonic treatment, microwave treatment, enzymolysis and hot water lixiviating to extract the active components of belly button snail polysaccharide and has high polysaccharide yield. The belly button snail polysaccharide may be used in develop functional food with the functions of regulating immunity, resisting cancer and inhibiting tumor.
Description
Technical field
The present invention relates to nutritional health food field, particularly a kind of polysaccharide that from the marine animal body, makes and be the functional foodstuff that having of making of activeconstituents regulated immunologic function, anticancer, function of tumor inhibition with this polysaccharide.
Background technology
Navel spiral shell meat and/or its tankage polysaccharide have high nutritive value and medical care effect, belong to the flavor nutrition material.Yet, seldom, the correlative study of pair navel spiral shell meat and/or its tankage polysaccharide is not arranged so far yet to the marine animal STUDY ON POLYSACHAROSE in the past.The method that natural polysaccharide extracts in other animal and plant bodies has traditionally: hot water extraction, acidleach formulation, alkali extraction, also have enzyme process etc.But because water is difficult to complete stripping polysaccharide material wherein as solvent, so need extracted many times, the operating time is long, and yield is low; Acidleach formulation and alkali extraction make part polysaccharide generation hydrolysis owing to acid, alkali concn are high, have destroyed the active structure of polysaccharide; And adopt Enzymatic Extraction polysaccharide yield also not high merely.
Summary of the invention
The object of the present invention is to provide a kind ofly to have the belly bottom snail polysaccharide of regulating immunologic function, anticancer, function of tumor inhibition, and the method that from navel spiral shell meat and/or its tankage, makes belly bottom snail polysaccharide.
It is the food compositions that is used to regulate immunologic function, anticancer, function of tumor inhibition of activeconstituents with this belly bottom snail polysaccharide that another purpose of the present invention has provided a kind of.
To be above-mentioned belly bottom snail polysaccharide and food compositions have purposes aspect the functional foodstuff of regulating immunologic function, anticancer, function of tumor inhibition in preparation to further aim of the present invention.
The technical solution adopted for the present invention to solve the technical problems is: a kind of belly bottom snail polysaccharide with health role is provided, be with navel spiral shell meat and/or its tankage through homogenate, enzymolysis, water carry, concentrate, alcohol precipitation, removing albumen, alcohol precipitation, lyophilize, to make purity be 25～55% belly bottom snail polysaccharide; The preparation method of this belly bottom snail polysaccharide may further comprise the steps:
A. raw material and processing: fresh navel spiral shell meat and/or its tankage water are rinsed well, drained;
B. homogenate: add the water of 0～30 times of quality in fresh navel spiral shell meat and/or its tankage, put into refiner, homogenate 15～30min gets homogenate; Perhaps that fresh navel spiral shell meat and/or its tankage are dry under 0～70 ℃ of condition, after be ground into Powderedly, add the water of 0.5～30 times of quality, mixing gets homogenate;
C. ultrasonication: place ultrasonic generator to vibrate 0～4h homogenate or/and with microwave treatment 0～10min of 50～600w;
D. enzymolysis: the neutral protease that in the homogenate that step c handled, adds specific activity of enzyme 600～1200u/g, in pH6.5～8.5,35～50 ℃ of enzymolysis 1～3h, go out and add the stomach en-of specific activity of enzyme 6000～12000u/g behind the enzyme, ℃ continuation enzymolysis 1～5h transfers pH6.5～7.5 then in pH1.0～3.5,35～50, the enzyme that goes out obtains navel spiral shell meat and/or its tankage hydrolyzed solution;
E. water is carried: hydrolyzed solution is heated to 70～100 ℃ of water carries 0.5～5h, extract 1～3 time, obtain the water extract;
F. concentrate: it is 15～30% that the water extract is concentrated into solid quality content;
G. alcohol precipitation: in concentrated solution, add 95% (quality) ethanol of 2～6 times of volumes, leave standstill 8～24h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
H. remove albumen: the water that in throw out, adds 0.5～3 times of volume, 0.1 the chloroform of～0.5 times of volume: the chloroform amyl alcohol solution or the butanol solution of amylalcohol=4: 1～5: 1 (volume), violent jolting 20～30min, centrifugal, the denatured protein of branch vibration layer and solvent layer intersection obtains polysaccharide solution;
I. alcohol precipitation: in polysaccharide solution, add 95% (quality) ethanol of 2～6 times of volumes, leave standstill 8～24h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
J. lyophilize: at first in precipitation, add the water of 0.1～0.5 times of quality, be cooled to-30～-35 ℃, freeze fully, then at vacuum tightness 50～80Pa, 30～60 ℃ of plate temperature, dry 10～15h, obtain polysaccharide dry powder, polysaccharide yield is 3.0～16.0%, and purity is 25.0～55.0%.
Wherein the enzyme condition of going out in the steps d can be to be warming up to 90～95 ℃ to keep 3～10min; Or microwave deactivating enzyme 5～10min; It can be vacuum concentration under 45～70 ℃ of temperature that step f concentrates; Also can be to adopt the ultra-filtration membrane of MWC0100000 at 0.3～0.5MPa, 45～50 ℃ concentrate down.
Raw material of the present invention is representative with the navel spiral shell, is raw material but do not limit with other ocean snail, adopts preparation method of the present invention to prepare other ocean snail and prepares the snail polysaccharide.
The above-mentioned belly bottom snail polysaccharide that functional foodstuff composition of the present invention contains treatment or prevention effective dose is an activeconstituents, and contains one or more field of food acceptable carriers.
Belly bottom snail polysaccharide of the present invention and food compositions can be used for preparing and have the protective foods of regulating immunologic function, anticancer, function of tumor inhibition.
Above-mentioned described field of food acceptable carrier is meant the food carriers of field of food routine, comprises thinner, vehicle such as water etc.; Weighting agent such as starch, sucrose etc.; Tamanori such as Mierocrystalline cellulose and derivative, gelatin; Wetting agent such as glycerine; Can also in composition, add other assistant agent such as sweeting agent, flavouring agent etc.
Belly bottom snail polysaccharide of the present invention can be taken usefulness with the form through port of composition, brings into play it and regulates immunologic function, anticancer, the effect that suppresses tumour.Also can be according to the conventional production method of food or medicine field, as make its activeconstituents and one or more carriers or medicament mixed, preparing various formulations such as tablet, electuary, capsule, suppository, sprays etc., preferred form is tablet, electuary and capsule; Functional foodstuff composition of the present invention contains the belly bottom snail polysaccharide of the present invention that mass ratio is 1%-99.5%, and preferred purity is 5%～50% belly bottom snail polysaccharide.
The present invention compared with prior art has following advantage:
1. the present invention has fully utilized ultrasonic wave, microwave technology, enzymolysis linkage heat flooding technology, ultra-filtration technique, Freeze Drying Technique, can preserve original physiologically active substance in navel spiral shell meat and/or the tankage to greatest extent, and utilize freeze drying technology, make the finished product trophicity and functional having concurrently.
2. the present invention is a raw material with navel spiral shell meat and/or tankage, utilizes the combinative enzyme hydrolysis technology of optimum collocation, and the extraction yield of polysaccharide and purity are improved widely.
3. the present invention removes albumen twice by the method and the Sevag method of enzymolysis, has greatly improved the purity of polysaccharide.
4. the present invention is developed to polysaccharide product to the utilization of navel spiral shell meat and/or tankage and with it, both can be used as functional ocean food, can be used as base-material again, be aided with the multiple functional food of other food development, make the functional diversification day by day of protective foods, can recycle according to invention in the past for residue.
By food and biological new and high technology with navel spiral shell meat and/or tankage produce the polysaccharide goods, the degree of depth has transformed Living marine resources, and its economic benefit, environmental benefit and social benefit are significantly improved.
Embodiment
The following examples can help those skilled in the art more fully to understand the present invention, but do not limit the present invention in any way.
Embodiment 1.
A. raw material and processing: 50 kilograms of fresh meat water of navel spiral shell are rinsed well, drained;
B. homogenate: fresh navel spiral shell meat is put into refiner, and homogenate 15min gets homogenate;
C. enzymolysis: in navel spiral shell meat homogenate, add enzyme activity 3 * 10
7The neutral protease of u, ℃ enzymolysis 1h in pH6.5～7.0,35～38 is warming up to 90 ℃ and keeps the 3min enzyme that goes out, and it is 3 * 10 that the cooling back adds enzyme activity
8The stomach en-of u continues enzymolysis 1h in pH1.0～1.5,35～38 ℃, transfers pH6.5 then, is warming up to 95 ℃ and keeps the 3min enzyme that goes out, and obtains navel spiral shell meat hydrolyzed solution;
D. water is carried: hydrolyzed solution is heated to 70 ℃ of water carries 5h, obtain the water extract;
E. concentrate: is 15% in 45 ℃ of vacuum concentration to containing solid quality content;
F. alcohol precipitation: in concentrated solution, add 95% (quality) ethanol of 2 times of volumes, leave standstill 8h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
G. remove albumen: in throw out, add the water of 0.5 times of volume, the chloroform of 0.5 times of volume: the chloroform amyl alcohol solution of amylalcohol=4: 1 (volume), violent jolting 20min; Centrifugal, the denatured protein of branch vibration layer and solvent layer intersection obtains polysaccharide solution;
H. alcohol precipitation: in polysaccharide solution, add 95% (quality) ethanol of 6 times of volumes, leave standstill 24h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
I. lyophilize: at first in precipitation, add the water of 0.1 times of quality, be cooled to-30～-32 ℃, freeze fully, then at vacuum tightness 50～60Pa, 30～35 ℃ of plate temperature, dry 15h obtains polysaccharide dry powder, and polysaccharide yield is 16%, and purity is 40%.
Embodiment 2.
A. raw material and processing: 50 kilograms of fresh navel spiral shell tankage waters are rinsed well, drained;
B. homogenate: navel spiral shell tankage are put into refiner, and homogenate 30min gets homogenate;
C. enzymolysis: in navel spiral shell tankage homogenate, add enzyme activity 6 * 10
7The neutral protease of u, ℃ enzymolysis 2h in pH7.0～7.5,40～42 is warming up to 93 ℃ and keeps the 7min enzyme that goes out, and it is 6 * 10 that the cooling back adds enzyme activity
8The stomach en-of u continues enzymolysis 3h in pH3.0～3.5,45～50 ℃, transfers pH7.0 then, is warming up to 95 ℃ and keeps the 10min enzyme that goes out, and obtains navel spiral shell tankage hydrolyzed solution;
D. water is carried: hydrolyzed solution is heated to 100 ℃ of water carries 0.5h, obtain the water extract, extract repeatedly 3 times;
E. concentrate: is 30% in 70 ℃ of vacuum concentration to containing solid quality content;
F. alcohol precipitation: in concentrated solution, add 95% (quality) ethanol of 4 times of volumes, leave standstill 24h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
G. remove albumen: in throw out, add the water of 3 times of volumes, the chloroform of 0.1 times of volume: the chloroform amyl alcohol solution of amylalcohol=5: 1 (volume), violent jolting 30min; Centrifugal, the denatured protein of branch vibration layer and solvent layer intersection obtains polysaccharide solution;
H. alcohol precipitation: in polysaccharide solution, add 95% (quality) ethanol of 4 times of volumes, leave standstill 8h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
I. lyophilize: at first in precipitation, add the water of 0.5 times of quality, be cooled to-33～-35 ℃, freeze fully, then at vacuum tightness 60～70Pa, 45～50 ℃ of plate temperature, dry 10h obtains polysaccharide dry powder, and polysaccharide yield is 3.0%, and purity is 55%.
Embodiment 3.
A. raw material and processing: 50 kilograms of fresh meat water of navel spiral shell are rinsed well, drained;
B. homogenate: dry under 70 ℃ of conditions, after be ground into Powderedly, add 1500 kilograms water, mixing gets homogenate;
Make polysaccharide dry powder as embodiment 1 step c, d, e, f, g, h, I, polysaccharide yield is 16.0%, and purity is 45.0%.
Embodiment 4.
A. raw material and processing: 50 kilograms of fresh navel spiral shell meat and tankage water are rinsed well, drained;
B. homogenate: navel spiral shell meat and tankage are put into refiner, and homogenate 30min gets homogenate;
C. ultrasonication: navel spiral shell meat and tankage homogenate are placed on the 2h that vibrates in the ultrasonic generator with the microwave treatment 6min of 300w;
D. enzymolysis: add the neutral protease of enzyme activity 4.5 * 107u in navel spiral shell meat and tankage homogenate, ℃ enzymolysis 3h in pH8.0～8.5,48～50 is warming up to 93 ℃ and keeps the 7min enzyme that goes out, and it is 6 * 10 that the cooling back adds enzyme activity
8The stomach en-of u/g, in pH3.5,50 ℃ are continued hydrolysis 5h, transfer pH7.5 then, are warming up to 95 ℃ and keep the 10min enzyme that goes out, and obtain navel spiral shell meat and tankage hydrolyzed solution;
E. water is carried: hydrolyzed solution is heated to 85 ℃ of water carries 3h, obtain the water extract, extract repeatedly 2 times;
F. concentrate: is 22% in 60 ℃ of vacuum concentration to containing solid quality content;
G. alcohol precipitation: in concentrated solution, add 95% (quality) ethanol of 6 times of volumes, leave standstill 16h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
H. remove albumen: in throw out, add the water of 2.5 times of volumes, the chloroform of 0.3 times of volume: the chloroform amyl alcohol solution of amylalcohol=5: 1 (volume), violent jolting 25min; Centrifugal, the denatured protein of branch vibration layer and solvent layer intersection obtains polysaccharide solution;
I. alcohol precipitation: in polysaccharide solution, add 95% (quality) ethanol of 2 times of volumes, leave standstill 16h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
J. lyophilize: at first in precipitation, add the water of 0.3 times of quality, be cooled to-32～-34 ℃, freeze fully, then at vacuum tightness 70～80Pa, 55～60 ℃ of plate temperature, dry 12h, obtain 5760 gram polysaccharide dry powder, polysaccharide yield is 11.52%, and purity is 45.41%.
Embodiment 5
A. raw material and processing: 50 kilograms of fresh navel spiral shell tankage waters are rinsed well, drained;
B. homogenate: navel spiral shell tankage are put into refiner, add 1500 kg water, homogenate 20min gets homogenate;
C. microwave treatment: with the microwave treatment 12min of navel spiral shell tankage homogenate with 600w;
D. enzymolysis: in navel spiral shell tankage homogenate, add the neutral protease of enzyme activity 4.5 * 107u, ℃ enzymolysis 3h in pH8.0～8.5,48～50, adding enzyme activity behind the microwave deactivating enzyme 5min is 6 * 10
8The stomach en-of u/g, in pH3.5,50 ℃ are continued hydrolysis 5h, transfer pH7.5 then, and microwave deactivating enzyme 10min obtains navel spiral shell tankage hydrolyzed solution;
E. water is carried: hydrolyzed solution is heated to 85 ℃ of water carries 3h, obtain the water extract, extract repeatedly 2 times;
F. concentrate: is 15% in 60 ℃ of vacuum concentration to containing solid quality content;
G. alcohol precipitation: in concentrated solution, add 95% (quality) ethanol of 4 times of volumes, leave standstill 16h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
H. remove albumen: in throw out, add the water of 2 times of volumes, the propyl carbinol of 0.5 times of volume, violent jolting 25min; Centrifugal, the denatured protein of branch vibration layer and solvent layer intersection obtains polysaccharide solution;
I. alcohol precipitation: in polysaccharide solution, add 95% (quality) ethanol of 2 times of volumes, leave standstill 16h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
J. lyophilize: at first in precipitation, add the water of 0.3 times of quality, be cooled to-32～-34 ℃, freeze fully, then at vacuum tightness 70～80Pa, 55～60 ℃ of plate temperature, dry 12h obtains 3800 gram polysaccharide dry powder, and polysaccharide yield is 7.6%, and purity is 25.0%.
Embodiment 6
A. raw material and processing: 50 kilograms of fresh meat water of navel spiral shell are rinsed well, drained;
B. homogenate: seasoning, after be ground into Powderedly, add 25 kilograms water, mixing gets homogenate;
C. ultrasonication: with the navel spiral shell meat homogenate 4h that in ultrasonic generator, vibrates;
D. enzymolysis: add the neutral protease of enzyme activity 5.0 * 107u in navel spiral shell meat homogenate, ℃ enzymolysis 3h in pH8.0～8.5,48～50 is warming up to 93 ℃ and keeps the 7min enzyme that goes out, and it is 6 * 10 that the cooling back adds enzyme activity
8The stomach en-of u/g, in pH3.5,50 ℃ are continued hydrolysis 5h, transfer pH7.5 then, are warming up to 95 ℃ and keep the 10min enzyme that goes out, and obtain navel spiral shell meat hydrolyzed solution;
E. water is carried: hydrolyzed solution is heated to 85 ℃ of water carries 3h, obtain the water extract, extract repeatedly 2 times;
F. concentrate: the ultra-filtration membrane that adopts MWCO100000 is at 0.3～0.5MPa, is concentrated in the time of 45～50 ℃ that to contain solid quality content be 30%;
G. alcohol precipitation: in concentrated solution, add 95% (quality) ethanol of 4 times of volumes, leave standstill 16h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
H. remove albumen: in throw out, add the water of 3 times of volumes, the propyl carbinol of 0.4 times of volume, violent jolting 25min; Centrifugal, the denatured protein of branch vibration layer and solvent layer intersection obtains polysaccharide solution;
I. alcohol precipitation: in polysaccharide solution, add 95% (quality) ethanol of 2 times of volumes, leave standstill 16h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
J. lyophilize: at first in precipitation, add the water of 0.3 times of quality, be cooled to-32～-34 ℃, freeze fully, then at vacuum tightness 70～80Pa, 55～60 ℃ of plate temperature, dry 12h obtains 3050 gram polysaccharide dry powder, and polysaccharide yield is 3.0%, and purity is 44.6%.
The capsular preparation of embodiment 7. belly bottom snail polysaccharides: get belly bottom snail polysaccharide 10 grams of embodiment 1 preparation, add the edible cyclodextrin 10g of vehicle, mix, granulation incapsulates.
The preparation of embodiment 8. belly bottom snail polysaccharide tablets: get belly bottom snail polysaccharide 10 grams of embodiment 3 preparations, add the edible cyclodextrin 10g of vehicle, mix, the granulation compressing tablet makes tablet.
The preparation of embodiment 9. belly bottom snail polysaccharide electuaries: get belly bottom snail polysaccharide 10 grams of embodiment 2 preparations, add the edible cyclodextrin 10g of vehicle, mix granulation, the packing bag of packing into.
Claims (8)
1. belly bottom snail polysaccharide with health role, it is characterized in that with navel spiral shell meat and/or its tankage through homogenate, enzymolysis, water carry, concentrate, alcohol precipitation, removing albumen, alcohol precipitation, lyophilize, to make purity be 25～55% belly bottom snail polysaccharide.
2. the preparation method of the described belly bottom snail polysaccharide of claim 1 is characterized by and may further comprise the steps:
A. raw material and processing: fresh navel spiral shell meat and/or its tankage water are rinsed well, drained;
B. homogenate: add the water of 0～30 times of quality in fresh navel spiral shell meat and/or its tankage, put into refiner, homogenate 15～30min gets homogenate; Perhaps that fresh navel spiral shell meat and/or its tankage are dry under 0～70 ℃ of condition, after be ground into Powderedly, add the water of 0.5～30 times of quality, mixing gets homogenate;
C. ultrasonication: place ultrasonic generator to vibrate 0～4h homogenate or/and with microwave treatment 0～10min of 50～600w;
D. enzymolysis: the neutral protease that in the homogenate that step c handled, adds specific activity of enzyme 600～1200u/g, in pH6.5～8.5,35～50 ℃ of enzymolysis 1～3h, go out and add the stomach en-of specific activity of enzyme 6000～12000u/g behind the enzyme, ℃ continuation enzymolysis 1～5h transfers pH6.5～7.5 then in pH1.0～3.5,35～50, the enzyme that goes out obtains navel spiral shell meat and/or its tankage hydrolyzed solution;
E. water is carried: hydrolyzed solution is heated to 70～100 ℃ of water carries 0.5～5h, extract 1～3 time, obtain the water extract;
F. concentrate: it is 15～30% that the water extract is concentrated into solid quality content;
G. alcohol precipitation: in concentrated solution, add 95% (quality) ethanol of 2～6 times of volumes, leave standstill 8～24h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
H. remove albumen: the water that in throw out, adds 0.5～3 times of volume, 0.1 the chloroform of～0.5 times of volume: the chloroform amyl alcohol solution or the butanol solution of amylalcohol=4: 1～5: 1 (volume), violent jolting 20～30min, centrifugal, the denatured protein of branch vibration layer and solvent layer intersection obtains polysaccharide solution;
I. alcohol precipitation: in polysaccharide solution, add 95% (quality) ethanol of 2～6 times of volumes, leave standstill 8～24h after, centrifugal 15min under the 4000r/min centrifugal condition, collecting precipitation thing;
J. lyophilize: at first in precipitation, add the water of 0.1～0.5 times of quality, be cooled to-30～-35 ℃, freeze fully, then at vacuum tightness 50～80Pa, 30～60 ℃ of plate temperature, dry 10～15h, obtain polysaccharide dry powder, polysaccharide yield is 3.0～16.0%, and purity is 25～55%.
3. the preparation method of belly bottom snail polysaccharide according to claim 2, the enzyme condition of going out that it is characterized by in the steps d keeps 3～10min for being warming up to 90～95 ℃.
4. the preparation method of belly bottom snail polysaccharide according to claim 2, the enzyme condition of going out that it is characterized by in the steps d is microwave action 5～10min.
5. the preparation method of belly bottom snail polysaccharide according to claim 2, it is characterized by that step f concentrates is vacuum concentration under 45～70 ℃ of temperature.
6. the preparation method of belly bottom snail polysaccharide according to claim 2, it is characterized by step f concentrate adopt MWC0100000 ultra-filtration membrane at 0.3～0.5MPa, 45～50 ℃ concentrate down.
7. the belly bottom snail polysaccharide in the claim 1 has in the protective foods of regulating immunologic function, anticancer, function of tumor inhibition in preparation and uses.
8. be used to regulate the functional foodstuff composition of immunologic function, anticancer, function of tumor inhibition, it is characterized by the polysaccharide and the field of food acceptable carrier of the claim 1 that wherein contains treatment or prevention significant quantity.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102276751A (en) * | 2011-08-11 | 2011-12-14 | 天津科技大学 | Method for extracting glycosaminoglycan from bullacta exarata |
| CN106084086A (en) * | 2016-08-03 | 2016-11-09 | 淮阴工学院 | The method for removing protein of Cipangopaludina chinensis polysaccharide extraction liquid |
| CN111205376A (en) * | 2020-01-07 | 2020-05-29 | 华侨大学 | Preparation method and application of wart litchi and snail polysaccharide |
| CN113388044A (en) * | 2020-03-11 | 2021-09-14 | 中国科学院昆明植物研究所 | Snail glycosaminoglycan compound, pharmaceutically acceptable salt thereof, preparation method and application |
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2005
- 2005-10-11 CN CNB2005100474117A patent/CN100494221C/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102276751A (en) * | 2011-08-11 | 2011-12-14 | 天津科技大学 | Method for extracting glycosaminoglycan from bullacta exarata |
| CN102276751B (en) * | 2011-08-11 | 2012-07-25 | 天津科技大学 | Method for extracting glycosaminoglycan from bullacta exarata |
| CN106084086A (en) * | 2016-08-03 | 2016-11-09 | 淮阴工学院 | The method for removing protein of Cipangopaludina chinensis polysaccharide extraction liquid |
| CN111205376A (en) * | 2020-01-07 | 2020-05-29 | 华侨大学 | Preparation method and application of wart litchi and snail polysaccharide |
| CN111205376B (en) * | 2020-01-07 | 2021-11-02 | 华侨大学 | Preparation method and application of wart litchi and snail polysaccharide |
| CN113388044A (en) * | 2020-03-11 | 2021-09-14 | 中国科学院昆明植物研究所 | Snail glycosaminoglycan compound, pharmaceutically acceptable salt thereof, preparation method and application |
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