CN1717982A - Container used for culturing test-tube seedling of oriental China fir - Google Patents
Container used for culturing test-tube seedling of oriental China fir Download PDFInfo
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- CN1717982A CN1717982A CN 200510028184 CN200510028184A CN1717982A CN 1717982 A CN1717982 A CN 1717982A CN 200510028184 CN200510028184 CN 200510028184 CN 200510028184 A CN200510028184 A CN 200510028184A CN 1717982 A CN1717982 A CN 1717982A
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- china fir
- oriental
- container
- test
- tube seedling
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Abstract
A container for culturing the test-tube plantlet of eastern fir has a smooth top mouth able to be enclosed by an air-permeable aseptic film. Its advantages are high growth speed, strong plant and high budding rate.
Description
Technical field
The present invention relates to a kind of container that test-tube seedling of oriental China fir is cultivated that is used for.
Background technology
Be with adding lid container during test-tube seedling of oriental China fir was cultivated in the past, its culture effect is undesirable.The invention provides a kind of new culture vessel for this reason.
Goal of the invention
The object of the invention is to provide a kind of culture vessel that utilizes test-tube seedling of oriental China fir.
The object of the invention can be achieved through the following technical solutions: a kind of container that is used for the test-tube seedling of oriental China fir cultivation, characteristics are: upper opening of container is smooth, seals with aseptic breathable films at place suitable for reading.
In order further to improve the growing environment of east China fir test-tube plantlet, can offer air-vent on the described ventilated membrane, at the air-vent place bacterium filter membrane is set.
The present invention and prior art are than superiority: east China fir test-tube plantlet individual plant on average increased height 2.32cm ± 0.71cm in 35 days; Seedling is sturdy tall and big, and side shoot is many, mounted blade; Stem section sprout rate 1: 1.5; The growing state of bud is general early stage, and the back growth of 3 weeks is very fast.
Description of drawings
Fig. 1: ventilative aseptic plastic seals film
Fig. 2: test-tube seedling of oriental China fir situation in the bottle of different vessels lid
Embodiment
The aseptic plastic of breathing freely as Fig. 1 seals shown in the membrane structure, a kind of container 1 that is used for the test-tube seedling of oriental China fir cultivation, and container 1 is suitable for reading smooth, seals 2 at place suitable for reading with the aseptic breathable films of PTFE.Wherein, air-vent 21 is arranged on ventilated membrane, 21 places are provided with bacterium filter membrane 3 at air-vent.
In order to promote the growth of east China fir tissue cultivating seedling, present embodiment comes the capping container with different container cover in the China fir test-tube plantlet incubation in the Orient, a kind of is air-locked plastic closure, a kind of is the plastic closures film of breathing freely, and has compared the influence of container ventilation property to test-tube seedling of oriental China fir thereby analyze.
One, experiment material
1, east China fir test-tube plantlet individual plant 200 strains, 200 sections of stem sections.
2, see Fig. 1 container of the present invention: 40 of vinyl covers; There are ventilative bacterium filter membrane 3 seals of a hole 21 usefulness, 40 in blake bottle 1 aseptic ventilative film PTFE 2 centres of sealing.
Two, experimental technique
1, preparation 40 bottles of D-1 individual plant growth mediums (MHJ+ activated carbon 1-5mg/l), wherein 20 bottle containers seal with vinyl cover, and 20 bottle containers seal film with aseptic plastic and seal to seal with the rubber band handle again and tighten; 40 bottles of D-2 stem section growth mediums:
(MHJ+6-BA0.2-1.0mg/l+ activated carbon 1-5mg/l), in kind every kind is sealed 20 bottles on lid.
2, the individual plant of the high 1.5cm-2.0cm of inoculation on the D-1 medium, every bottle 5 strain; The stem section about the long 1.5cm of inoculation on the D-2 medium, 5 sections every bottle.
3, the test-tube plantlet that inoculation is good is cultivated in culturing room, and cultivation temperature is 25 ℃ ± 2 ℃, and light application time is about 15h every day.
4, respectively the test-tube plantlet in the different vessels is measured the height of every strain after the inoculation, measured its height after 35 days again; Equally the stem section is measured the height of extracting sprouting out and added up the quantity of sprouting after 35 days.
Four, experimental result
Added up the average growing height of individual plant after cultivating 35 days, observed the growing state of individual plant: sturdy degree, blade open situation and collateral generation situation or the like; After cultivating 35 days, added up equally the sprout rate of stem section, and the growing state that sprouts of stem Duan Xin.The growing state statistics of seedling sees Table 1 in the container of two kinds of different lids.
Table 1: container ventilation property is to the sprout statistics of influence of east China fir individual plant and stem section
| The container closure material | Individual plant on average increased height in 35 days | The individual plant growing state | Stem section sprout rate | The growing state of bud |
| Vinyl cover | 1.31cm±0.50cm | Seedling is short and small thin and delicate, and side shoot is seldom arranged, the blade hard-pressed bale | 1∶1.5 | Growth is general |
| The plastic closures film | 2.32cm±0.71cm | Seedling is sturdy tall and big, and side shoot is many, mounted blade | 1∶1.5 | Early stage is general, and the back growth of 3 weeks is very fast |
Five, interpretation of result
The gas permeability of experimental result demonstration culture vessel has very big effect to the growth of east China fir, seal film with breathable plastic and help bottle interior gas and outside exchange gas, can make entering of carbon dioxide in the extraneous air, the carbonic acid gas because of photosynthesis consumption in the growth of east China fir is replenished.With breathable plastic seal the height that the film individual plant on average increases and improved 77%, and seedling stalwartness, side shoot are many, blade is unfolded, and see test-tube seedling of oriental China fir situation in the bottle of Fig. 2 different vessels lid.And short and small thin and delicate with the seedling in common air-locked plastic closure container, side shoot is seldom arranged, the blade hard-pressed bale.The lid gas permeability is broken up not influence to stem section bud, just extracts out in the container of sprouting at good permeability in the later stage and grows more hurry up.
Claims (3)
1. one kind is used for the container that test-tube seedling of oriental China fir is cultivated, and it is characterized in that: upper opening of container is smooth, seals with aseptic breathable films at place suitable for reading.
2. the container that is used for the test-tube seedling of oriental China fir cultivation according to claim 1 is characterized in that: on the described ventilated membrane air-vent is arranged, at the air-vent place bacterium filter membrane is set.
3. the container that is used for the test-tube seedling of oriental China fir cultivation according to claim 1 and 2 is characterized in that: described ventilated membrane is poly-tetrafluoro alkene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510028184 CN1717982A (en) | 2005-07-27 | 2005-07-27 | Container used for culturing test-tube seedling of oriental China fir |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510028184 CN1717982A (en) | 2005-07-27 | 2005-07-27 | Container used for culturing test-tube seedling of oriental China fir |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1717982A true CN1717982A (en) | 2006-01-11 |
Family
ID=35930026
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510028184 Pending CN1717982A (en) | 2005-07-27 | 2005-07-27 | Container used for culturing test-tube seedling of oriental China fir |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1717982A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104822256A (en) * | 2012-10-11 | 2015-08-05 | 瑞克斯旺种苗集团公司 | Vegetables, mushrooms or herbs in box |
-
2005
- 2005-07-27 CN CN 200510028184 patent/CN1717982A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104822256A (en) * | 2012-10-11 | 2015-08-05 | 瑞克斯旺种苗集团公司 | Vegetables, mushrooms or herbs in box |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |