CN1711883A - Production of vegetable protein - Google Patents
Production of vegetable protein Download PDFInfo
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- CN1711883A CN1711883A CN 200410059629 CN200410059629A CN1711883A CN 1711883 A CN1711883 A CN 1711883A CN 200410059629 CN200410059629 CN 200410059629 CN 200410059629 A CN200410059629 A CN 200410059629A CN 1711883 A CN1711883 A CN 1711883A
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- rice
- seed dregs
- vegetable protein
- barbadosnut seed
- protein production
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Abstract
A process for preparing the vegetative protein from the seed dregs of Jatropha curcas tree, which are generated by squeezing oil, includes such steps as diluting the seed dregs by water, adding modifier (the mixed solution of H2O2 and NH4OH), heating to 100-120 deg.C and detoxicating reaction. Said vegetative protein can be used as the additive of feed.
Description
Technical field
The present invention relates to a kind of production method of vegetable protein, the production method of particularly a kind of Jatropha curcas (Jatropha curcas L.) vegetable protein.
Background technology
China is a large agricultural country, and aquaculture is occupied crucial status in agricultural production.Feed industry is to depend on agricultural, serves the new industry of aquaculture, and after the reform and opening-up, the intensivization development of aquaculture is swift and violent.Introduce according to " national feed industrial development outline ", the whole nation in 2003 the feed annual production that is mixed reaches more than 100,000,000 ton, will reach 1.8 hundred million tons in 2010.Yet the shortage of feed resource, especially protein feed resources is the key factor of restriction China feed industrial development always.China will spend the up to ten million ton of huge foreign exchange import protein feed every year.Therefore, develop domestic plant protein fodder resource and become the task of top priority.Relevant department all classifies the new protein feed resources of exploitation as the key project of scientific research.Particularly for our such populous nation, what use is made of existing resource, particularly non-edible plant resource are developed protein feed, have more special meaning.
Jatropha curcas (Jatropha curcas L.) is Euphorbiaceae (Euphobia L.) Jatropha curcas platymiscium, and machaka or dungarunga are a kind of perennial woody oilseed plants.Jatropha seed oil content is very high, and oil yield is up to 40%.The Jatropha curcas plantation does not account for the arable land easily, the survival rate height, and planting cost is low.Distributed more widely in China, be particularly suitable for the dry-hot valley area plantation that economize in the Sichuan, Guizhou, Yunnan, Guangxi etc. in China southwest.In China's western development Jatropha curcas industry, for the ecological construction in dry-hot valley area, water and soil conservation has positive effect.Simultaneously can promote the west area structural readjustment of rural industry, quicken development of rural economy.To regional aid-the-poor project,, all be of great importance to increasing the poverty-stricken area farmers' income.The development of Jatropha curcas industry will bring huge economy effect, social benefit and ecological benefits.The barbadosnut seed dregs of rice of barbadosnut seed remainder after extracting oil contain rich in protein, crude protein content 〉=44%, and amino acid content and dregs of beans are suitable even be better than dregs of beans, are a kind of vegetable proteins of high-quality.However, owing to contain multiple noxious material in the barbadosnut seed dregs of rice, according to Chinese Center for Disease Control, occupational health and the control of poisoning related data, contain a certain amount of toxalbumin in the barbadosnut seed, its toxicity and ricin are similar, also contain noxious materials such as a small amount of hydrogen cyanide, ligustrazine in addition.Therefore, the plant protein material of this high-quality is not to be taken as fuel to burn for a long time, is used as fertilizer exactly.This is a kind of waste really.Development along with the intensive culture industry, and Jatropha curcas comprehensive utilization of resources, particularly in recent years, exploitation along with curcas biological diesel oil, the output of the barbadosnut seed dregs of rice will be increasing, the deep processing and utilization of the barbadosnut seed dregs of rice and industrialized developing have very huge commercial value and vast market prospect.
Certainly, vegetable protein is handled through fine finishining, can also be as the proteins additive of some food, even as medicine etc.
Utilize the castor-oil plant dregs of rice (residue after the castor bean oil expression), rapeseed dregs etc. to produce vegetable protein, become prior art through detoxification process.But utilize the barbadosnut seed dregs of rice to produce vegetable protein, be not subjected to people's attention.
Summary of the invention
The technical problem to be solved in the present invention just provides a kind of method of utilizing the barbadosnut seed dregs of rice to produce vegetable protein.
The technical solution used in the present invention is a kind of vegetable protein production method; With the plant waste matter is raw material, produces albumen through detoxification process; It is characterized in that: described raw material is Jatropha curcas (Jatropha curcas L.) the seed dregs of rice;
The described barbadosnut seed dregs of rice are the barbadosnut seed dregs of rice through the cold press de-oiling;
Described detoxification process comprises the steps:
A) behind the modified barbadosnut seed dregs of rice thin up, add adjusting material; Described adjusting material is equivalent H
2O
2(30% aqueous solution) and NH
4OH (20% aqueous solution) mixed liquor; Every 100kg barbadosnut seed dregs of rice add water 10～30kg, adjusting material 0.5～3kg;
B) add thermal response through the modified barbadosnut seed dregs of rice, be heated to 100～120 ℃, carry out detoxification reaction;
Described vegetable protein is used to produce feed.
The invention has the beneficial effects as follows, adopt the suitability for industrialized production technology, is raw material with the barbadosnut seed dregs of rice, produces vegetable protein.It is low to have cost of material, technical maturity, and production cost is low, is fit to the characteristics of large-scale production.
The specific embodiment
Below in conjunction with embodiment, describe technical scheme of the present invention in detail.
Technical scheme of the present invention is that vegetable protein detoxification process, equipment that utilization is ripe are raw material with the barbadosnut seed dregs of rice, produce vegetable protein.
Embodiment
After Jatropha curcas bore fruit, pericarp was yellow and can gathers.The Jatropha curcas of gathering is really carried out artificial or machine barking, obtain barbadosnut seed, require peeling rate 〉=99%.Barbadosnut seed makes moisture＜6% through airing, and the barbadosnut seed shell is light tone darkly, can put in storage.
1. preliminary treatment
1.1 remove impurity
By cleaning, screening, selection by winnowing, magnetic separation etc., remove the various impurity in the barbadosnut seed.
1.2 peel off
Unit mahine is peeled off-separated to utilization with the shell strip off of barbadosnut seed, and separating husk benevolence, obtains barbadosnut seed benevolence.Require hulling rate 〉=95%.
After the above-mentioned requirements processing, reduced coarse-fibred content in the barbadosnut seed dregs of rice, guarantee crude protein content 〉=44% in the barbadosnut seed dregs of rice.
2. oil expression
In order to guarantee the nutritional labeling in the barbadosnut seed dregs of rice, the present invention recommends to adopt the cold-press oil expression.In order to reduce the content of various oleic acid in the barbadosnut seed dregs of rice, require barbadosnut seed dregs of rice oil content≤8%, the recommendation index is oil content≤5%, oil content≤3% is better.
The oil expression of employing cold pressing process, barbadosnut seed dregs of rice oil content generally 〉=10% for reaching These parameters, can adopt impregnation technology, reduces oil content.
3. detoxification
3.1 the barbadosnut seed dregs of rice are modified
Through the barbadosnut seed dregs of rice that above-mentioned technology obtains, add water and adjusting material is modified.Modified proportioning is: the barbadosnut seed dregs of rice: water: adjusting material=100: 17: 1 (weight ratio) mixes.Adjusting material is equivalent H
2O
2(30% aqueous solution) and NH
4OH (20% aqueous solution) mixed liquor.
Amount of water is more flexible, generally adds water 15～30kg by the 100kg barbadosnut seed dregs of rice and is advisable, and adds water very little, and material is difficult for mixing influences detoxification efficiency; Add dilutional hyponatremia, not only the waste water resource and the energy also are unfavorable for the processing of subsequent technique; The recommendation amount of water is 15～20kg.The consumption of adjusting material is: the 100kg barbadosnut seed dregs of rice add 0.5～3kg adjusting material, addition very little, detoxification is incomplete; Excessively then can increase remaining quantity, influence product quality; Recommended amounts is 0.8～1.5kg.
3.2 add thermal response
The barbadosnut seed dregs of rice enter the hot blast retort after modified and carry out detoxification reaction.Retort is made up of double-layered cylinder, central principal axis and stir-fry plate.Material places inner core, is the hot air circulation space between the inner/outer tube, fries plate and inner core and presses the rightabout rotation, has avoided the material deposition with adherent.Hot blast is circulation repeatedly between inner/outer tube and hot-blast stove, and material constantly stirs, advances at inner core, homogeneous heating, and it is stable to heat up, and has guaranteed detoxification efficiency.Hot-blast stove makes heat circulation in closed circuit, becomes directly to be heated to be indirect.With hot blast rate control material temperature, the control system sensitivity, easy and simple to handle, avoided material to pollute or detonation, also improved product quality.Temperature of charge is controlled at 110～115 ℃, and fluidised form is moved about 1～3 minute in thermal field, stirs, mixes, and sufficient reacting changes the molecular structure of toxin, reaches the detoxification purpose.
Reaction temperature is controlled at 100～120 ℃, has both guaranteed detoxification speed and effect, can keep the nutritional labeling of the barbadosnut seed dregs of rice again as far as possible.Recommended temperature range is 110～115 ℃.The time of material in thermal field is unsuitable long, in order to avoid the nutritional labeling loss, had better not be above 15 minutes.
3.3 material cooling
Cooling system is that the secondary negative pressure is air-cooled, has stopped the finished product loss, has overcome and easily fire drawback, has improved output, has reduced cost, has improved the sanitary condition of workshop.
4. extrusion
Cooled material moisture is controlled at 10～25%, carries out extrusion in bulking machine, and bulking machine discharging opening temperature stabilization is in 100～120 ℃.
Jatropha curcas vegetable protein through extruding-puffing technique is handled has improved product quality, is more conducive to animal digestion and absorbs.Simultaneously, remove part moisture, also further improved detoxification efficiency.
5. dry cooling
Baking is dry down at 120～125 ℃, then cooling.Also should notice that stoving time should not grow.
6. broken
The cooled expanded material of drying is crushed to 0.5～3mm, promptly gets the Jatropha curcas plant protein products.
Analysis of experiments
Toxalbumin in the barbadosnut seed dregs of rice belongs to amino acid and arranges special protein, and sex change is general protein under wet heat condition, becomes nutrition.Its boiling point of a spot of hydrogen cyanide has only 26.5 ℃ in the barbadosnut seed dregs of rice, the hydrogen cyanide elimination of at high temperature volatilizing.A spot of ligustrazine is a kind of alkaloid in the barbadosnut seed dregs of rice, combines with ammonium ion in the adjusting material in retort and extrusion pressing puffing process and loses toxicity.
Test by analysis, detoxification efficiency sees Table 1.
Table 1 barbadosnut seed dregs of rice detoxification efficiency
| Virus elimination rate % after the detoxification before the detoxification of poisonous substance title |
| Ligustrazine (wt%) 0.29～0.38 0.01～0.012 99.9 above hydrogen cyanide (wt%) 0.08～0.11 0.00 100 |
The Jatropha curcas vegetable protein ingredient detects, and sees Table 2.
Detection index (wt%) after the detoxification of the table 2 barbadosnut seed dregs of rice
| Color and luster | Yellowish or dark yellow |
| Smell | Fry fragrance |
| Outward appearance | Uniform powder |
| Crude protein | ≥44 |
| Crude fibre | ≤5.5 |
| Moisture | ≤7.6 |
| Coarse ash | ≤8 |
| L-aminobutanedioic acid | 3.13 |
| Threonine | 1.92 |
| Serine | 1.75 |
| Glutamic acid | 4.55 |
| Proline | 1.39 |
| Glycine | 2.23 |
| Alanine | 1.63 |
| Cystine | 0.83 |
| Valine | 1.98 |
| Methionine | 0.64 |
| Isoleucine | 1.55 |
| Leucine | 2.41 |
| Tyrosine | 1.29 |
| Phenylalanine | 2.13 |
| Lysine | 2.44 |
| Histidine | 0.99 |
| Arginine | 3.75 |
Acute toxicity test (mouse)
Quote pharmacology teaching and research room of West China Center of Medical Sciences of Sichuan University toxicity chamber " survey report ".
1. barbadosnut protein pulvis animal acute toxicity test
Test method
It is 17.46% that the crazy seeds of trees dregs of rice of sample detoxification are prepared maximum suspended matter concentration with 0.5% first class cellulose, irritates the stomach pin with No. 12 and irritates stomach.Select 40 of body weight 18～22g one-level kunming mices that West China Center of Medical Sciences experimental animal center provides for use, with equilibrium at random two step method be divided into 4 groups, 10 every group, male and female half and half.Press listed each group (i.g.QdXI) of dosage contamination of table 3, contamination back mouse is freely looked for food, drinks water, and observes poisoning symptom at any time.
Result of the test
Mouse finds no unusual and dead after irritating the stomach contamination.Observed for 1 week, the results are shown in Table 3.
Table 3 is respectively organized dosage and animal dead number (death toll/contamination number)
| ??????2.15g/kg??????4.64g/kg???????10.0g/kg??????21.5g/kg |
| Female 0/5 0/5 0/5 0/5 male 0/5 0/5 0/5 0/5 |
Check conclusion
The mouse LD of this sample
50＞15.0g/kg, by " toxicological evaluation of food safety procedure ", it is nontoxic that this product belongs to.
2. not detoxification barbadosnut protein pulvis acute toxicity test
Test method
With sample not the crazy seeds of trees dregs of rice of detoxification to prepare maximum suspended matter concentration with 0.5% first class cellulose be 17.46%, irritate the stomach pins with No. 12 and irritate stomaches.Select 40 of body weight 18～22g one-level kunming mices that West China Center of Medical Sciences experimental animal center provides for use, with equilibrium at random two step method be divided into 4 groups, 10 every group, male and female half and half.Press listed each group (i.g.QdXI) of dosage contamination of table 4, contamination back mouse is freely looked for food, drinks water, and observes poisoning symptom at any time.
Result of the test
Mouse is in that to irritate stomach contamination back activity, diet, stool and urine all normal.Mouse had death in 1,2,3 day respectively at the contamination back, observed for 1 week, the results are shown in Table 4.
Table 4 group dosage and animal dead number (death toll/contamination number)
| ???????????2.15g/kg??????4.64g/kg??????10.0g/kg??????21.5g/kg |
| Female 0/5 1/5 3/5 5/5 male 0/5 0/5 4/5 5/5 |
Check conclusion
The dead mouse number of this sample is big, and it is poisonous that this product belongs to.
Test by analysis as can be seen, by the detoxification Jatropha curcas vegetable protein that above-mentioned technical process is produced, is a kind of nontoxic high-quality, and the vegetable protein suitable with the dregs of beans composition can be widely used in feedstuff industry, as the protein additive of all feeds.Utilize Jatropha curcas plant protein products of the present invention, adopt conventional feed production technology, can prepare and produce multiple feed product, repeat no more herein.
Claims (10)
1, a kind of vegetable protein production method; With the plant waste matter is raw material, produces albumen through detoxification process; It is characterized in that: described raw material is Jatropha curcas (Jatropha curcas L.) the seed dregs of rice.
2, vegetable protein production method according to claim 1 is characterized in that: the described barbadosnut seed dregs of rice are the barbadosnut seed dregs of rice through the cold press de-oiling.
3, vegetable protein production method according to claim 1 and 2, it is characterized in that: described detoxification process comprises the steps:
A) behind the modified barbadosnut seed dregs of rice thin up, add adjusting material; Described adjusting material is equivalent H
2O
2(30% aqueous solution) and NH
4OH (20% aqueous solution) mixed liquor; Every 100kg barbadosnut seed dregs of rice add water 10～30kg, adjusting material 0.5～3kg;
B) add thermal response through the modified barbadosnut seed dregs of rice, be heated to 100～120 ℃, carry out detoxification reaction.
4, vegetable protein production method according to claim 3 is characterized in that: among the described step a, every 100kg barbadosnut seed dregs of rice add water 15～20kg, adjusting material 0.8～1.5kg; Among the described step b, heating-up temperature is 110～115 ℃.
5, according to claim 3 or 4 described vegetable protein production methods, it is characterized in that: also comprise the extrusion step; The barbadosnut seed dregs of rice through detoxification treatment carry out extrusion in bulking machine.
6, vegetable protein production method according to claim 5 is characterized in that: in the described extrusion step, be controlled at 10～25% through the barbadosnut seed dregs of rice moisture of detoxification treatment, bulking machine discharging opening temperature stabilization carries out extrusion at 100～120 ℃.
7, according to the described vegetable protein production method of above-mentioned any claim, it is characterized in that: described barbadosnut seed dregs of rice oil content≤8%.
8, vegetable protein production method according to claim 7 is characterized in that: described barbadosnut seed dregs of rice oil content≤5%.
9, vegetable protein production method according to claim 8 is characterized in that: described barbadosnut seed dregs of rice oil content≤3%.
10, according to the described vegetable protein production method of above-mentioned any claim, it is characterized in that: described vegetable protein is used to produce feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200410059629XA CN100502679C (en) | 2004-06-14 | 2004-06-14 | Production of vegetable protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200410059629XA CN100502679C (en) | 2004-06-14 | 2004-06-14 | Production of vegetable protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1711883A true CN1711883A (en) | 2005-12-28 |
| CN100502679C CN100502679C (en) | 2009-06-24 |
Family
ID=35717807
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB200410059629XA Expired - Fee Related CN100502679C (en) | 2004-06-14 | 2004-06-14 | Production of vegetable protein |
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| Country | Link |
|---|---|
| CN (1) | CN100502679C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2466353A (en) * | 2008-12-15 | 2010-06-23 | D1 Oils Plant Science Ltd | Methods for detoxifying oil seed crops |
| CN102090512A (en) * | 2011-01-10 | 2011-06-15 | 中国海洋石油总公司 | Detoxication method for jatropha curcas and meal thereof |
| CN102090501A (en) * | 2010-10-27 | 2011-06-15 | 江南大学 | Method for detoxifying barbadosnut seed dregs and preparing protein concentrates |
| FR2955589A1 (en) * | 2010-01-26 | 2011-07-29 | Arkema France | PROCESS FOR REACTIVE TRITURATION OF JATROPHA SEEDS |
| CN102907628A (en) * | 2012-11-08 | 2013-02-06 | 四川大学 | Method for detoxifying jatropha curcas cakes |
| CN105211619A (en) * | 2014-05-28 | 2016-01-06 | 吴光平 | A kind of vegetable protein production method |
-
2004
- 2004-06-14 CN CNB200410059629XA patent/CN100502679C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2466353A (en) * | 2008-12-15 | 2010-06-23 | D1 Oils Plant Science Ltd | Methods for detoxifying oil seed crops |
| GB2466353B (en) * | 2008-12-15 | 2010-11-03 | D1 Oils Plant Science Ltd | Methods for detoxifying oil seed crops |
| FR2955589A1 (en) * | 2010-01-26 | 2011-07-29 | Arkema France | PROCESS FOR REACTIVE TRITURATION OF JATROPHA SEEDS |
| WO2011092430A1 (en) * | 2010-01-26 | 2011-08-04 | Arkema France | Method for reactively crushing jatropha seeds |
| CN102802433A (en) * | 2010-01-26 | 2012-11-28 | 阿肯马法国公司 | Method for reactively crushing jatropha seeds |
| CN107011990A (en) * | 2010-01-26 | 2017-08-04 | 阿肯马法国公司 | The method that reactivity crushes manioca seed |
| CN102090501A (en) * | 2010-10-27 | 2011-06-15 | 江南大学 | Method for detoxifying barbadosnut seed dregs and preparing protein concentrates |
| CN102090501B (en) * | 2010-10-27 | 2012-11-14 | 江南大学 | Method for detoxifying barbadosnut seed dregs and preparing protein concentrates |
| CN102090512A (en) * | 2011-01-10 | 2011-06-15 | 中国海洋石油总公司 | Detoxication method for jatropha curcas and meal thereof |
| CN102907628A (en) * | 2012-11-08 | 2013-02-06 | 四川大学 | Method for detoxifying jatropha curcas cakes |
| CN105211619A (en) * | 2014-05-28 | 2016-01-06 | 吴光平 | A kind of vegetable protein production method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100502679C (en) | 2009-06-24 |
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