CN1709220A - Cordyceps sinensis polypeptide nano series skin cosmetics and preparing method thereof - Google Patents
Cordyceps sinensis polypeptide nano series skin cosmetics and preparing method thereof Download PDFInfo
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- CN1709220A CN1709220A CN 200510016899 CN200510016899A CN1709220A CN 1709220 A CN1709220 A CN 1709220A CN 200510016899 CN200510016899 CN 200510016899 CN 200510016899 A CN200510016899 A CN 200510016899A CN 1709220 A CN1709220 A CN 1709220A
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Abstract
The present invention relates to a cordyceps polypeptide nano series skin-protecting cosmetic and its preparation method. Said skin-protecting cosmetic is formed from main agent, matrix and water. Said main agent is cordyceps emulsion concentrate containing biological activity substances of polypeptide, amino acids and D-mannitol, etc. Said invention can be made into various skin-protecting cosmetics with different dosage forms and different functions.
Description
Technical field
The invention belongs to the cosmetics field, specially referring to the pure Lac Bovis seu Bubali of defat is that substrate prepares Scarlet Caterpiller Fungus milk concentrated solution, wherein contained polysaccharide, aminoacid, bioactive substances such as D-mannitol are the main body composition, produce polypeptide class Moisture factor through protease hydrolysis, after nanotechnology treatments technology, form product host, by different needs through after adjuvant emulsifying, making the series of products of different dosage form.
Background technology
Cordyceps militaris is the Clavicipitaceae fungus, is the famous and precious rare mushroom of medicine-food two-purpose.It contains polysaccharide, D-mannitol, Fructus Hordei Germinatus sterol, several amino acids and trace element.Recent study is found, people are separated to the various active material from Cordyceps militaris and phorozoon thereof, have antibiotic, antitumor, radioprotective, the anti-ageing pharmacological action of waiting for a long time as these bioactive substances such as adenosine class, polysaccharide, alkaloids, cyclic peptide class, enzymes.At present relevant Cordyceps militaris and exploration project is more concentrates in the health food also has to set foot at cosmetic field, but all is to adopt traditional processing technology, and the raw material of selecting for use is all sporophore or mycelium and pulverizes to add and form.
Learn according to related data, on May 21st, 1994, by proposing with artificial medicinal Cordyceps in (number of patent application is 94106027.6) " Chinese caterpillar fungus cosmetics " of the Wang Jingshan application of Grand View Tower village, Kunming, Yunnan is raw material, be aided with other adjuvant and make, its main technique is Cordyceps sporophore or extract to be added in the existing cosmetics make.Equally disclosedly in the patent (application number is 01108817.6) " superfine cordyceps cosmetics and production method thereof " of application on October 21st, 1996 be by him, " a kind of superfine cordyceps cosmetics; it is characterized in that containing pure or compound superfine cordyceps ", addition " is advisable with about 0.1～0.5% " usually; Specific practice is still the production method of " adding an amount of pure or compound superfine cordyceps in various cosmetics ".
Although be in cosmetics, to add superfine plant worm powder, still can not rapid osmotic skin, strengthen the skin moisture-keeping effect.
Summary of the invention:
The technical problem to be solved in the present invention is, utilize skimmed milk to cultivate the Cordyceps militaris emulsion, with bio protease be hydrolyzed the preparation moisture-keeping function biological peptide, utilize nanotechnology that the biotic factor of macromolecular structure is carried out new technique new technologies such as classification ultramicronising processing, be greatly improved thereby make the rapid osmotic of product strengthen moisture-keeping functions
Cordyceps militaris breast cosmetics of the present invention and manufacturing process thereof, mainly be to utilize the skimmed milk fermentation to produce to contain Cordyceps polysaccharide, the fermentation milk of multiple bioactive substances such as D-mannitol is a host, and adopts protease that the protein in the Cordyceps militaris breast host is hydrolyzed to form the peptide matters with preserving moisture and protecting skin effect.Take nanotechnology to carry out advanced technologies such as biomacromolecule ultramicronising processing after concentrating, being equipped with corresponding substrate adjuvant at last carries out mixing and emulsifying, sterilization, and fill is a different dosage form, the Cordyceps serial cosmetics of different efficacies.
Cordyceps sinensis polypeptide nano series skin cosmetics of the present invention is made up of substrate and water, and said substrate comprises softening agent, hygroscopic agent, emulsifying agent, thickening agent, antiseptic and spice; It is characterized in that it is Scarlet Caterpiller Fungus milk concentrated solution that host, host are arranged in the composition; The consumption of Scarlet Caterpiller Fungus milk concentrated solution accounts for 2-10% by mass percentage.
Above-mentioned Scarlet Caterpiller Fungus milk concentrated solution is through skimmed milk fermentation, protease is hydrolyzed and ultramicronising obtains after handling cordyceps sinensis fermentation liquor.Become 1/2～1/4 of original volume through vacuum concentration, be the flowable state thick liquid, and contain the nanometer level bioactive material.Wherein main nutrient composition is a cordycepic acid 4～10%, amino acid/11 8～22%, cordycepin 0.2～3%.
Above-mentioned substrate is identical with the component that existing cosmetics use basically.For example use stearic acid, silicone oil to make softening agent, use lanonol, glycerol, triethanolamine to make hygroscopic agent, use hexadecanol, glyceryl monostearate to make emulsifying agent, use white oil to make thickening agent, use Bronopol to make antiseptic, use rose essence to make spice or the like.The consumption of each composition is also identical with existing cosmetics substantially in the substrate.
The preparation method of Chinese caterpillar fungus polypeptide nano series skin cosmetics of the present invention is, through inoculation Cordyceps militaris bacterium, use protease hydrolysis, concentrate that ultramicronising is handled, the emulsive technical process of batch mixing prepares skin protection cosmetics;
Said inoculation Cordyceps militaris bacterium process is that access Cordyceps militaris strain was cultivated 48～120 hours under 22～28 ℃ of temperature in aseptic skimmed milk culture medium, again to the mycelial cell ultrasonication, filtered and obtained the Cordyceps militaris breast;
Said protease hydrolysis process is, adding enzyme activity to the Cordyceps militaris Ruzhong under 50～60 ℃ of conditions is that the papain of 30000～50000U is or/and trypsin, enzyme concentration is 0.034～0.9mg/ml, stirs hydrolysis down 30～60 minutes, refilters to obtain Cordyceps militaris breast hydrolyzed solution;
Said concentrated ultramicronising processing procedure is, utilizes the vacuum concentration method that Cordyceps militaris breast hydrolyzed solution is concentrated into 1/2～1/4 of original volume, makes to contain cordycepic acid 4～10% in the Scarlet Caterpiller Fungus milk concentrated solution; Put into nanometer machine ultramicronising again and handle, make its biomacromolecule reach 100～200nm, make Scarlet Caterpiller Fungus milk concentrated solution;
Said batch mixing emulsion process is a mixed-matrix by mass percentage, and stirring and emulsifying adds the sterilization of Scarlet Caterpiller Fungus milk concentrated solution mixing again.
Above-mentioned inoculation Cordyceps militaris bacterium process in the ratio access Cordyceps militaris strain of skimmed milk volume 3%, is cultivated under 65 times/minute frequency stirring conditions; Can expect in environment that also wind is than the following cultivation of 1: 0.5～1 (v/v).Incubation time is by the survival rate decision of Cordyceps militaris strain, and incubation time was 72～96 hours scopes preferably.
When the vacuum concentration method concentrated Cordyceps militaris breast hydrolyzed solution, vacuum was preferably 9.4 * 10
4Pa.
In the batch mixing emulsion process, can be earlier the oil phase component in the substrate and water component be heated to 70～85 ℃ respectively after, mix and stir, make its complete emulsifying.Slowly cooling is cooled to 40～45 ℃ again, adds host Scarlet Caterpiller Fungus milk concentrated solution and antiseptic and essence, stirs evenly the back sterilization.
By the method for the invention, Scarlet Caterpiller Fungus milk fermented solution need pass through protease hydrolysis, make proteolysis in the Scarlet Caterpiller Fungus milk fermented solution become the biological peptide of micromolecule and cooperate the Organic substance of the multiple biologically active feature that produces in the fermentation liquid, can make the Cordyceps militaris breast increase the preserving moisture and protecting skin cosmetic result of skin.Concentrate after the hydrolysis, the ultramicronising PROCESS FOR TREATMENT reaches in 100～200nm scope in the nanometer machine, helps reaching the performance of its effect in the bioactie agent rapid osmotic skin.In various cosmetics, add Scarlet Caterpiller Fungus milk concentrated solution, make different prescriptions, different dosage form very easily, the cosmetics of different efficacies.
The specific embodiment:
Provide the specific embodiment of the present invention below, but do not mean limitation of the scope of the invention.
The host of embodiment 1 product of the present invention, substrates quantity scope by mass percentage:
(1) active component, Scarlet Caterpiller Fungus milk concentrated solution 2～10%, the best is 5～8%
(2) softening agent 1～30%
(3) hygroscopic agent 2～10%
(4) emulsifying agent 0.1～20%
(5) ropiness increasing agent 0.1～20%
(6) antiseptic 0.01～3%
(7) spice 0.1～5%
(8) surplus is a water.
One of best prescription of embodiment 2 products of the present invention, consumption be by mass percentage:
First component (oil phase component):
Lanonol 1.8%
Hexadecanol 1.6%
White oil 12.0%
Stearic acid (three press) 1.5%
Silicone oil 0.8%
Glyceryl monostearate 2.5%
Second component (water component):
Glycerol 6.0%
Triethanolamine 0.3%
Third component:
Scarlet Caterpiller Fungus milk concentrated solution 6.0%
Bronopol 0.01%
Rose essence 0.5%
Water surplus.
The prescription of present embodiment is the whitening skin and preserving moisture protective skin cream.Preparation process can be: after earlier first component and second component being heated 75～80 ℃ respectively, mix and stir, make its complete emulsifying.When slowly cooling is cooled to 40～45 ℃, add third component, stir evenly postcooling to room temperature.Under aseptic condition, carry out packing rapidly.
One of best prescription of embodiment 3 products of the present invention, consumption be by mass percentage:
The first component:
White oil 10%
Myristic acid isopropyl ester 4.5%
Lanoline 0.6%
Stearic acid (three press) 2.5%
The second component:
Glycerol 4.5%
Triethanolamine 0.8%
Third component:
Scarlet Caterpiller Fungus milk concentrated solution 5.0%
Essence 0.3%
Kazon 0.01%
Water surplus.
The prescription of present embodiment is for being fit to the whitening skin-protection cream of dry skin.Preparation process is with embodiment 2.
One of best prescription of embodiment 4 products of the present invention, consumption be by mass percentage:
The first component:
Lanoline 5%
Cera Flava 12%
Squalane 1.5%
White oil 33.5%
Paraffin 4.0%
Spermol 0.6%
The second component:
Borax 0.8%
The soft water surplus
Third component:
Essence 0.3%
Kazon 0.01%
Scarlet Caterpiller Fungus milk concentrated solution 8.0%.
The prescription of present embodiment is for being fit to the protective skin cream of various skins.Preparation process is with embodiment 2.
The preparation of embodiment 5 host raw material Scarlet Caterpiller Fungus milk concentrated solutions
The mycelial preparation of Cordyceps militaris breast (process of inoculation Cordyceps militaris bacterium):
Get a certain amount of skimmed milk ratio in 3% after 131 ℃/3S sterilization back is rapidly to 25 ℃ and insert the Cordyceps militaris strain, material wind was cultivated 72～96 hours than 1: 0.5～1.0 (v/v) or under 65 times/minute frequencies in 26 ℃ ± 2 ℃ environment, then through colloid mill and ultrasonic disruption thalline histiocyte, filter, standby.
The preparation of biological peptide in the host raw material (protease hydrolysis process):
Get the Cordyceps militaris emulsion after broken thalline histiocyte also filters, by volume mass ratio adds the papain 0.5mg/ml of enzyme activity 30000～50000U, slowly stirs hydrolysis 50 minutes under 55 ℃ of conditions, and filtration is standby.
The preparation of nanoscale Scarlet Caterpiller Fungus milk concentrated solution host (concentrating the ultramicronising processing procedure):
(vacuum is 9.4 * 10 will to utilize conventional vacuum concentration process through the Cordyceps militaris emulsion that contains biological polypeptide and other bioactive substance after the Protease Treatment
4Pa) be concentrated into 1/3 of original volume, ultramicronising PROCESS FOR TREATMENT in the nanometer machine reaches 100～200nm then.
The preparation of embodiment 6 Scarlet Caterpiller Fungus milk concentrated solutions
The incubation time of inoculation Cordyceps militaris bacterium among the embodiment 5 is determined also to be fine at 48～120 hours.
With the preparation of the biological peptide among the embodiment 5, proteolytic enzyme uses papain or/and trypsin, and enzyme concentration is in 0.034～0.9mg/ml scope, and hydrolysis time was 30～60 minutes scopes, and effect all is the same.
The emulsive technical process of embodiment 7 batch mixings prepares skin protection cosmetics
Prepare skin protection cosmetics by the process batch mixing emulsifying among the embodiment 1,2,3, also can reduce to 2.0% with the addition of host Scarlet Caterpiller Fungus milk concentrated solution is minimum, also the addition of host Scarlet Caterpiller Fungus milk concentrated solution can be added to 10% at most.
Claims (7)
1, a kind of cordyceps sinensis polypeptide nano series skin cosmetics is made up of substrate and water, and said substrate comprises softening agent, hygroscopic agent, emulsifying agent, thickening agent, antiseptic and spice; It is characterized in that it is Scarlet Caterpiller Fungus milk concentrated solution that host, host are arranged in the composition; The consumption of Scarlet Caterpiller Fungus milk concentrated solution accounts for 2～10% by mass percentage.
According to the described cordyceps sinensis polypeptide nano series skin cosmetics of claim 1, it is characterized in that 2, said Scarlet Caterpiller Fungus milk concentrated solution is the flowable state thick liquid; Contain cordycepic acid 4～10%.
According to claim 1 or 2 described cordyceps sinensis polypeptide nano series skin cosmetics, it is characterized in that 3, the composition of said substrate and consumption by mass percentage are, lanonol 1.8%, hexadecanol 1.6%, white oil 12.0%, stearic acid 1.5%, silicone oil 0.8%, glyceryl monostearate 2.5%, glycerol 6.0%, triethanolamine 0.3%, Bronopol 0.01%, rose essence 0.5%; Said host Scarlet Caterpiller Fungus milk concentrated solution consumption by mass percentage is 6.0%; Surplus is a soft water.
4, according to claim 1 or 2 described cordyceps sinensis polypeptide nano series skin cosmetics, it is characterized in that, the composition of said substrate and consumption by mass percentage are white oil 10%, myristic acid isopropyl ester 4.5%, lanoline 0.6%, stearic acid 2.5%, glycerol 4.5%, triethanolamine 0.8%, Kazon 0.01%, essence 0.3%; Said host Scarlet Caterpiller Fungus milk concentrated solution consumption by mass percentage is 5%; Surplus is a soft water.
5, according to claim 1 or 2 described cordyceps sinensis polypeptide nano series skin cosmetics, it is characterized in that the composition of said substrate and consumption by mass percentage are lanoline 5%, Cera Flava 12%, squalane 1.5%, white oil 33.5%, paraffin 4.0%, spermol 0.6%, Borax 0.8%, Kazon 0.01%, essence 0.3%; Said host Scarlet Caterpiller Fungus milk concentrated solution consumption by mass percentage is 8%; Surplus is a soft water.
6, a kind of preparation method of cordyceps sinensis polypeptide nano series skin cosmetics, cordyceps sinensis polypeptide nano series skin cosmetics is made up of substrate and water; It is characterized in that the host Scarlet Caterpiller Fungus milk concentrated solution is arranged in the composition, its consumption accounts for 2～10% by mass percentage; Prepare skin protection cosmetics through inoculation Cordyceps, protease hydrolysis, concentrated ultramicronising processing, the emulsive technical process of batch mixing;
Said inoculation Cordyceps militaris bacterium process is that access Cordyceps militaris strain was cultivated 48～120 hours under 22～28 ℃ of temperature in aseptic skimmed milk culture medium, again to the mycelial cell ultrasonication, filtered and obtained the Cordyceps militaris breast;
Said protease hydrolysis process is, under 50～60 ℃ of conditions, add the papain of enzyme activity 30000～50000U or/and trypsin to the Cordyceps militaris Ruzhong, enzyme concentration is 0.034～0.9mg/ml, stirs hydrolysis down 30～60 minutes, refilters to obtain Cordyceps militaris breast hydrolyzed solution;
Said concentrated ultramicronising processing procedure is, utilizes the vacuum concentration method that Cordyceps militaris breast hydrolyzed solution is concentrated into 1/2～1/4 of original volume, makes to contain cordycepic acid 4～10% in the Scarlet Caterpiller Fungus milk concentrated solution; Utilize the nanometer machine to carry out ultramicronising again and handle, make its biomacromolecule reach 100～200nm, make Scarlet Caterpiller Fungus milk concentrated solution;
Said batch mixing emulsion process is, mixed-matrix by mass percentage, and stirring and emulsifying adds the sterilization of Scarlet Caterpiller Fungus milk concentrated solution mixing again.
7, according to the preparation method of the described cordyceps sinensis polypeptide nano series skin cosmetics of claim 6, it is characterized in that, said inoculation Cordyceps militaris bacterium process, ratio in skimmed milk volume 3% inserts the Cordyceps militaris strain, stir or be under 1: 0.5～1.0 the ventilation condition at material wind ratio 65 times/minute frequencies, cultivated 72～96 hours; Said vacuum concentration method, vacuum are 9.4 * 10
4Pa.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194516A (en) * | 2013-03-17 | 2013-07-10 | 赤峰鼎基生物工程科技有限公司 | Preparation method of cordyceps militaris polypeptide |
| CN104323980A (en) * | 2014-11-23 | 2015-02-04 | 沈阳源康医疗器械有限公司 | Face cream containing cordyceps militaris ingredient and preparation method of face cream |
| CN105087401A (en) * | 2015-09-17 | 2015-11-25 | 成都朴锐生物科技有限公司 | Preparation method of artificial ophiocordyceps sobolifera |
| CN105560157A (en) * | 2015-12-29 | 2016-05-11 | 徐跃 | Novel application of Cordyceps fungus extract |
| CN105941628A (en) * | 2016-05-03 | 2016-09-21 | 内蒙古鼎祥健康产业投资有限公司 | Preparation method of health-care Chinese caterpillar fungus peptide milk for middle-aged and aged people |
| CN108852918A (en) * | 2018-05-21 | 2018-11-23 | 张全兴 | A kind of aerolite train of energy skin care item and preparation method thereof |
| CN109199949A (en) * | 2017-07-03 | 2019-01-15 | 葛运傑 | Chinese medical extract ultrafine particles atomized inhalation clearing lung-heat formula one |
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2005
- 2005-06-21 CN CNB2005100168997A patent/CN100493490C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194516A (en) * | 2013-03-17 | 2013-07-10 | 赤峰鼎基生物工程科技有限公司 | Preparation method of cordyceps militaris polypeptide |
| CN104323980A (en) * | 2014-11-23 | 2015-02-04 | 沈阳源康医疗器械有限公司 | Face cream containing cordyceps militaris ingredient and preparation method of face cream |
| CN105087401A (en) * | 2015-09-17 | 2015-11-25 | 成都朴锐生物科技有限公司 | Preparation method of artificial ophiocordyceps sobolifera |
| CN105560157A (en) * | 2015-12-29 | 2016-05-11 | 徐跃 | Novel application of Cordyceps fungus extract |
| CN105560157B (en) * | 2015-12-29 | 2021-01-15 | 徐跃 | Application of cordyceps fungus extract |
| CN105941628A (en) * | 2016-05-03 | 2016-09-21 | 内蒙古鼎祥健康产业投资有限公司 | Preparation method of health-care Chinese caterpillar fungus peptide milk for middle-aged and aged people |
| CN109199949A (en) * | 2017-07-03 | 2019-01-15 | 葛运傑 | Chinese medical extract ultrafine particles atomized inhalation clearing lung-heat formula one |
| CN108852918A (en) * | 2018-05-21 | 2018-11-23 | 张全兴 | A kind of aerolite train of energy skin care item and preparation method thereof |
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| CN100493490C (en) | 2009-06-03 |
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