CN1687814A - Fash washing encapsulating apparatus and method for glass slide - Google Patents
Fash washing encapsulating apparatus and method for glass slide Download PDFInfo
- Publication number
- CN1687814A CN1687814A CN 200510038726 CN200510038726A CN1687814A CN 1687814 A CN1687814 A CN 1687814A CN 200510038726 CN200510038726 CN 200510038726 CN 200510038726 A CN200510038726 A CN 200510038726A CN 1687814 A CN1687814 A CN 1687814A
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- slide holder
- inundator
- detersive
- microslide
- slide
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Abstract
It is the device which can rapidly clean and encapsulate the slide. The method is a kind of easy cleaning procedure, not needing to recurrently move and drag the slide. The device can be produced in batch mode. It includes the slide fixer (1) and the inundator (2). The method of rapid cleaning and encrusting is that the slide is inserted into the slot (13) and several slide fixers (1) are put into the inundator (2) side by side, while (2) is immersed into the cleanser cistern. Then take out (2) and put it on the recycle device for the cleanser to collect the cleanser. Several slide fixers (1) are put into the glass container to balance the pH value. Get (1) out and then pour 95% alcohol into the glass container to defat. After that, take it out to get it dry. At last, put (1) into the glass container which has been poured into the suitable encrusting solution. After encrusting, wash it in other glass containers and then dry it. Then keep it for use.
Description
Technical field
The present invention a kind ofly washs and wraps the device and method of quilt fast to microslide, belongs to the technical field of material preparation in the biomedicine.
Background technology
In histology, histochemistry and the histiocytic immune group of routine, fluorescent dye, in situ hybridization, microslide is the important carrier of biological sample.No matter be tradition or modern life science, microslide all is the instrument of using always.At present, the microslide of buying from producer often will pass through the washing of detersive (aqueous solution of the heavy cadmium acid potassium and the concentrated sulphuric acid), bag could use after by last adhesive, in case washing not exclusively, can on microslide, stay spot, make the microslide pH value seriously on the low side, if misoperation also can produce slide mark on microslide, this will influence the observations of experiment, even the phenomenon that biological sample comes off from microslide occur.The shortcoming of tradition microslide cleaning procedure is:
1, cleaning procedure is loaded down with trivial details, and cleaning efficiency is low; 2, in the cleaning course because being in contact with one another between the microslide, usually cause on the microslide detersive residual and PH is on the low side; 3, in the cleaning course because of spurring repeatedly, rubbing, slide surface very easily produces slide mark; 4, lack the retracting device of detersive, make the detersive that has heavy metal and strong acid directly enter sewer, cause environmental pollution; 5, water consumption is big, causes the waste of water resource; 6, convenient for mark, the one side or the two sides of present microslide have frosted, and through the immersion of detersive, frosted easily comes off, and pollute easily and carry glass; 7, the microslide bag is by consuming time long in the adhesive process.
Summary of the invention
Technical matters: it is simple to the purpose of this invention is to provide a kind of cleaning procedure, need not move, draw microslide repeatedly, and the microslide that can produce in batches washs the device and method of bag quilt fast.
Technical scheme: the device that microslide of the present invention washs the bag quilt fast comprises slide holder and inundator two parts, slide holder is made of the framework of a rectangular parallelepiped the fixator frame, be provided with one or more dividing plates in the intermediate vertical of fixator frame the inner space of fixator frame is separated into a plurality of spaces, the slot that is provided with in the both sides up and down of fixator frame and dividing plate symmetry, be respectively equipped with the slot baffle plate on the both sides of slot, above following window, be provided with positive stop strip; Inundator is a box-like rectangular parallelepiped bigger than slide holder, and slide holder can be placed in the inundator, and the suitable for reading of inundator opens wide, and is provided with side plate all around, and its negative is provided with hole, garden, many bottom surfaces.
Microslide of the present invention washs fast, the operating process of method for coating is as follows:
1), microslide is inserted in the slot of slide holder;
2), with a plurality of slide holder and drain in the inundator;
3), inundator is immersed the detersive pond;
4), take out inundator, place on the retracting device of detersive, with the detersive on watering can washed, slide holder and inundator surface, collect the detersive in the retracting device;
5), after the detersive for the treatment of microslide, slide holder surface cleans, a plurality of slide holder are put into the glassware of suitable size, fill with tap water or distilled water or deionized water or other solution on demand, equilibrated ph value;
6), slide holder is taken out, put into the glassware that injects 95% alcohol degreasing again, through about 24 hours, dry after the taking-up or dry up;
7), slide holder is put into the glassware of the coating buffer that injection on demand prepares, bag by after, in other glassware washing several times, dry or dry up, standby with preservative film, 4 ℃ of masking foil parcels or-20 ℃ of preservations.
Beneficial effect: these apparatus and method once can be washed bag simply fast by 200 microslides, common laboratory only need 2-5 to overlap this system can to satisfy experiment required, compare with traditional handling procedure, this device has very significantly advantage:
1, cleaning procedure is simple, need not move repeatedly, dilatory microslide, can produce in batches, and through according to a preliminary estimate, this system is higher more than 20 times than the efficient of classic method;
2, not have detersive residual for slide surface, can make the pH value of the pH value of slide surface and balance institute water in full accord;
3, slide surface does not have slide mark;
The retracting device that 4, detersive is arranged, recyclable 99% detersive is beneficial to environmental protection, and this retracting device can also be used for the recovery of other vessel cleaning agent;
5, water consumption only reaches 10% of classic method;
6, can avoid frosted particle contamination microslide on the microslide;
7, this bag can be made the microslide Fast Packet by adhesives such as last poly-D-lysine and APES by device, and can improve the utilization factor of adhesive, saves the consumption of adhesive.
Description of drawings
Fig. 1 is the structural representation of slide holder 1 of the present invention, and fixator frame 11, slot baffle plate 12 are wherein arranged; Slot 13, positive stop strip 16.
Fig. 2 is the structural representation of inundator 2 of the present invention, and hole, garden, bottom surface 21, negative 22, side plate 23 are wherein arranged.
Embodiment
The device that microslide of the present invention washs the bag quilt fast comprises slide holder 1 and inundator 2 two parts, slide holder 1 is made of the framework of a rectangular parallelepiped fixator frame 11, be provided with one or more dividing plates 14 in the intermediate vertical of fixator frame 11 inner space of fixator frame 11 is separated into a plurality of spaces, be provided with slot 13 in the symmetry of both sides up and down of fixator frame 11 and dividing plate 14, be respectively equipped with slot baffle plate 12 on the both sides of slot 13, above following window 17, be provided with positive stop strip 16; Inundator 2 is a box-like rectangular parallelepiped bigger than slide holder 1, and slide holder 1 can be placed in the inundator 2, and the suitable for reading of inundator 2 opens wide, and is provided with side plate 23 all around, and its negative 22 is provided with hole, garden, many bottom surfaces 21.
The method of washing bag quilt is fast: the equilibrated ph value, degreasing and the bag that are ready to the detersive pond of splendid attire detersive, the retracting device that reclaims detersive, certain specification are by the glassware of adhesive;
1), microslide is inserted in the slot 13 of slide holder 1;
2), with a plurality of slide holder 1 and drain in the inundator 2;
3), inundator 2 is immersed the detersive pond;
4), take out inundator 2, place on the retracting device of detersive, with the detersive on watering can washed, slide holder (1) and inundator 2 surfaces, collect the interior detersive of retracting device;
5), after the detersive for the treatment of microslide, slide holder 1 surface cleans, a plurality of slide holder 1 are put into the glassware of suitable size, fill with tap water or distilled water or deionized water or other solution on demand, equilibrated ph value; Take out (about 24 hours) behind the certain hour, changes water several times or solution midway;
6), slide holder 1 is taken out, put into the glassware degreasing that fills 95% alcohol again, (about 24 hours) are dried after the taking-up or are dried up behind the certain hour, if research object is the RNA of biological sample, can directly do roasting in 180 ℃;
7), slide holder 1 is put into the glassware of the coating buffer that injection on demand prepares, bag by after, in other glassware washing several times, dry or dry up, standby with preservative film, 4 ℃ of masking foil parcels or-20 ℃ of preservations.Coating buffer is reusable.
Claims (2)
1, a kind of microslide washs the device of bag quilt fast, it is characterized in that this device comprises slide holder (1) and inundator (2) two parts, slide holder (1) is made of the framework of a rectangular parallelepiped fixator frame (11), be provided with one or more dividing plates (14) in the intermediate vertical of fixator frame (11) inner space of fixator frame (11) is separated into a plurality of spaces, be provided with slot (13) in the symmetry of both sides up and down of fixator frame (11) and dividing plate (14), be respectively equipped with slot baffle plate (12) on the both sides of slot (13), be provided with positive stop strip (16) in the top of following window (17); Inundator (2) is a box-like rectangular parallelepiped bigger than slide holder (1), slide holder (1) can be placed in the inundator (2), the suitable for reading of inundator (2) opens wide, and is provided with side plate (23) all around, and its negative (22) is provided with hole, garden, many bottom surfaces (21).
2, a kind of microslide as claimed in claim 1 washs the quick washing method for coating of the device of bag quilt fast, it is characterized in that the method for washing bag quilt is fast:
1), microslide is inserted in the slot (13) of slide holder (1);
2), with a plurality of slide holder (1) and drain in the inundator (2);
3), inundator (2) is immersed the detersive pond;
4), take out inundator (2), place on the retracting device of detersive, with the detersive on watering can washed, slide holder (1) and inundator (2) surface, collect the interior detersive of retracting device;
5), after the detersive for the treatment of microslide, slide holder (1) surface cleans, a plurality of slide holder (1) are put into the glassware of suitable size, fill with tap water or distilled water or deionized water or other solution on demand, equilibrated ph value;
6), slide holder (1) is taken out, put into the glassware degreasing that fills 95% alcohol again, through about 24 hours, dry after the taking-up or dry up;
7), slide holder (1) is put into the glassware of the coating buffer that injection on demand prepares, bag by after, in other glassware washing several times, dry or dry up, standby with preservative film, 4 ℃ of masking foil parcels or-20 ℃ of preservations.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510038726 CN1687814A (en) | 2005-04-07 | 2005-04-07 | Fash washing encapsulating apparatus and method for glass slide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510038726 CN1687814A (en) | 2005-04-07 | 2005-04-07 | Fash washing encapsulating apparatus and method for glass slide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1687814A true CN1687814A (en) | 2005-10-26 |
Family
ID=35305856
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510038726 Pending CN1687814A (en) | 2005-04-07 | 2005-04-07 | Fash washing encapsulating apparatus and method for glass slide |
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| CN (1) | CN1687814A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7753352B2 (en) | 2006-01-13 | 2010-07-13 | Hon Hai Precision Industry Co., Ltd. | Holding member for cleaning optical components |
| CN100998985B (en) * | 2006-01-14 | 2010-07-28 | 鸿富锦精密工业（深圳）有限公司 | Cleaning device for optical element |
| CN103331285A (en) * | 2013-06-24 | 2013-10-02 | 句容骏成电子有限公司 | Insertion basket |
| CN104785485A (en) * | 2013-07-16 | 2015-07-22 | 重庆医药高等专科学校 | Method for cleaning histochemistry test glass slide |
| CN107015417A (en) * | 2017-06-05 | 2017-08-04 | 浙江舜宇光学有限公司 | The method of anti-dazzling screen delustring |
| CN110238107A (en) * | 2019-07-17 | 2019-09-17 | 辽东学院 | A kind of multi-functional automatically washing and sterilizing slide device |
-
2005
- 2005-04-07 CN CN 200510038726 patent/CN1687814A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7753352B2 (en) | 2006-01-13 | 2010-07-13 | Hon Hai Precision Industry Co., Ltd. | Holding member for cleaning optical components |
| CN100998985B (en) * | 2006-01-14 | 2010-07-28 | 鸿富锦精密工业（深圳）有限公司 | Cleaning device for optical element |
| CN103331285A (en) * | 2013-06-24 | 2013-10-02 | 句容骏成电子有限公司 | Insertion basket |
| CN103331285B (en) * | 2013-06-24 | 2016-02-17 | 句容骏成电子有限公司 | A kind of plug in basket |
| CN104785485A (en) * | 2013-07-16 | 2015-07-22 | 重庆医药高等专科学校 | Method for cleaning histochemistry test glass slide |
| CN104785486A (en) * | 2013-07-16 | 2015-07-22 | 重庆医药高等专科学校 | Cleaning method for microscopic slide touching with cedar oil stain |
| CN107015417A (en) * | 2017-06-05 | 2017-08-04 | 浙江舜宇光学有限公司 | The method of anti-dazzling screen delustring |
| CN110238107A (en) * | 2019-07-17 | 2019-09-17 | 辽东学院 | A kind of multi-functional automatically washing and sterilizing slide device |
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