CN104785485A - Method for cleaning histochemistry test glass slide - Google Patents

Method for cleaning histochemistry test glass slide Download PDF

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Publication number
CN104785485A
CN104785485A CN201510124817.4A CN201510124817A CN104785485A CN 104785485 A CN104785485 A CN 104785485A CN 201510124817 A CN201510124817 A CN 201510124817A CN 104785485 A CN104785485 A CN 104785485A
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CN
China
Prior art keywords
slide
glass slide
conveyer belt
conveying belt
paper
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201510124817.4A
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Chinese (zh)
Other versions
CN104785485B (en
Inventor
刘怡
景戌
王惠
邱妍川
韦丽佳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chongqing Medical and Pharmaceutical College
Original Assignee
Chongqing Medical and Pharmaceutical College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chongqing Medical and Pharmaceutical College filed Critical Chongqing Medical and Pharmaceutical College
Priority to CN201510124817.4A priority Critical patent/CN104785485B/en
Publication of CN104785485A publication Critical patent/CN104785485A/en
Application granted granted Critical
Publication of CN104785485B publication Critical patent/CN104785485B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B11/00Cleaning flexible or delicate articles by methods or apparatus specially adapted thereto
    • B08B11/04Cleaning flexible or delicate articles by methods or apparatus specially adapted thereto specially adapted for plate glass, e.g. prior to manufacture of windshields
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B1/00Cleaning by methods involving the use of tools
    • B08B1/10Cleaning by methods involving the use of tools characterised by the type of cleaning tool
    • B08B1/14Wipes; Absorbent members, e.g. swabs or sponges
    • B08B1/143Wipes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04Cleaning involving contact with liquid
    • B08B3/08Cleaning involving contact with liquid the liquid having chemical or dissolving effect
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B7/00Cleaning by methods not provided for in a single other subclass or a single group in this subclass

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cleaning In General (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Re-Forming, After-Treatment, Cutting And Transporting Of Glass Products (AREA)

Abstract

The invention discloses a method for cleaning a histochemistry test glass slide. The cleaning method comprises the steps that the glass slide is placed at the starting end of a conveying belt, and the upper surface of the glass slide is stained with cedar oil; the conveying belt conveys the glass slide to the first position for pause; the upper surface of the glass slide is covered with lens wiping paper; the conveying belt conveys the glass slide to the second position for pause; ether is dripped on the lens wiping paper covering the upper surface of the glass slide; when the conveying belt conveys the glass slide to the third position, the lens wiping paper covering the upper surface of the glass slide is taken down; the conveying belt continuously conveys the glass slide, and the glass slide falls into a collecting basket below the tail end of the conveying belt. An automatic microscope glass slide cleaning device comprises the conveying belt supporting and conveying the microscope glass slide, a conveying belt stepping motor, a paper delivering system, a paper taking system and a cleaning liquid dripping device, the work efficiency is improved, contact between workers and harmful substances is reduced, and obvious benefits are brought.

Description

A kind of clean method of histochemical test slide
the present patent application is the applying date is on 07 16th, 2013, application number is the divisional application of 201310296114.0.
Technical field
The present invention relates to laboratory apparatus field, concrete is a kind of microslide clean method and device.
Background technology
In the histology, the biochemical test such as histochemistry and histiocytic immunohistochemistry, fluorescent staining, in situ hybridization of routine, slide is the important carrier of sample, and the slide for microscope oil mirror diagosis needs to drip cedar oil to change refractive index thus to improve picture quality.
Slide after using speckles with arborvitae oil stain because of its upper surface, needs to be dissolved with ether or the reagent such as natalite or dimethylbenzene.Otherwise the oil stain of dry after coagulation on slide can have a strong impact on the picture quality of next diagosis.But all there is the serious harm to human body in the reagent such as ether.
At present, the clean main laboratory worker that relies on speckling with the slide of cedar oil drips reagent on slide, and make it dissolve cedar oil and then wiped clean, its operating efficiency is low,, there is serious potential safety hazard in laboratory worker Long contact time harmful substance.Therefore, provide a kind of efficiency high, avoid the microslide cleaning solution of laboratory worker Long contact time harmful substance just to seem particularly important and urgent.
Summary of the invention
An object of the present invention is to provide the high microslide clean method of a kind of cleaning efficiency.
The technical scheme adopted for realizing the object of the invention is such, and a kind of microslide automatic cleaning method, comprises the following steps:
1) slide is positioned over the initiating terminal of conveyer belt, the upper surface of described slide speckles with cedar oil.
2) slide is sent to primary importance by described conveyer belt, and pauses.Lens wiping paper is covered at described slide upper surface.
3) slide is sent to the second place by described conveyer belt, and pauses.Ether is dripped to the lens wiping paper covering slide upper surface.
4), when slide is sent to the 3rd position by described conveyer belt, the lens wiping paper bar covering slide upper surface is taken off.
5) described conveyer belt continues to transmit slide, and slide falls into the collection basket be arranged in below conveyer belt end.
In method of the present invention, only need marginally to drip ether equal solvent to the lens wiping paper covered on slide, and after ether dissolution cedar oil oil stain, the mixture of oil stain and ether is present in lens wiping paper, only need to handle lens wiping paper well, just can not cause a large amount of ether volatilizations in atmosphere.
Another object of the present invention is to provide a kind of microslide automatic cleaning apparatus realizing said method, and this device comprises and supports and transmit the conveyer belt of microslide, conveyer belt stepper motor, transfer system, paper pickup system and cleaning solution Dropping feeder.Described conveyer belt is by driving stepper motor, and described cleaning solution Dropping feeder is positioned at above conveyer belt.Described transfer system is arranged on the side of conveyer belt, and described transfer system comprises paper breaking mechanism and ADF.Described ADF is equipped with bar shaped paper tape, and described paper breaking mechanism comprises the paper knife and chopping block that cooperatively interact.After described bar shaped paper tape is sent by ADF, first through the gap described paper knife and chopping block, then cover the upper surface of conveyer belt.When slide is sent to below bar shaped paper tape by described conveyer belt, bar shaped paper tape cuts off by described paper knife, makes the one section of bar shaped paper tape be cut off cover the upper surface of slide.Described cleaning solution Dropping feeder, built with ether, time below the dropper that the slide covering bar shaped paper tape is sent to cleaning solution Dropping feeder, drips ether by described dropper to bar shaped paper tape.What described paper pickup system comprised the side being arranged on conveyer belt pinches rod, when slide be sent to described pinch rod concordant position time, one section of bar shaped paper tape that described slide covers is clamped rear hauling-out by the described rod that pinches.
The technique effect of device of the present invention is mathematical, owing to have employed automatic transfer system, cleaning solution Dropping feeder and paper pickup system, achieve and the batch of slide is cleaned, improve operating efficiency, decrease the contact of staff and harmful substance, bring significant benefit.Further, device of the present invention can be placed in confined space, more effectively prevents ether to evaporate in working environment, reduces work risk.
Accompanying drawing explanation
The nonlimiting examples that device of the present invention can be provided by accompanying drawing further illustrates.
Fig. 1 is structural representation of the present invention;
Fig. 2 is the schematic diagram that slide is in " primary importance ";
Fig. 3 is the schematic diagram that slide is in " second place ";
Fig. 4 is the schematic diagram that slide is in " the 3rd position ".
In figure: 1-conveyer belt, 2-dropper, 3-ADF, 4-paper breaking mechanism, 5-installing rack, 6-upper roller, 7-lower roller.
Detailed description of the invention
Below in conjunction with drawings and Examples, the invention will be further described, but should not be construed the above-mentioned subject area of the present invention and be only limitted to following embodiment.Without departing from the idea case in the present invention described above, according to ordinary skill knowledge and customary means, make various replacement and change, all should be included in the scope of the present invention.
embodiment 1:
The present embodiment discloses a kind of microslide automatic cleaning method, comprises the following steps:
1) slide is positioned over the initiating terminal of conveyer belt, the upper surface of described slide speckles with cedar oil;
2) slide is sent to primary importance by described conveyer belt, and pauses; Lens wiping paper is covered at described slide upper surface;
3) slide is sent to the second place by described conveyer belt, and pauses; Ether is dripped to the lens wiping paper covering slide upper surface;
4) when slide is sent to the 3rd position by described conveyer belt, can pause or not stop, the lens wiping paper bar covering slide upper surface is taken off;
5) described conveyer belt continues to transmit slide, and slide falls into the collection basket be arranged in below conveyer belt end.
What deserves to be explained is, in step 2 in the present embodiment ~ 4, described primary importance, the second place and the 3rd position are three nonoverlapping positions between conveyer belt initiating terminal and end, and slide, after the upper surface being placed in conveyer belt 2, passes through this three positions successively.Described " pause " is conveyer belt 2 stop motion, can pass through step motor control.When slide is sent to this three positions, all to pauses, operate to facilitate.The area of described lens wiping paper bar is suitable with the area of slide upper surface, but also can be greater than the area of slide upper surface.In the step 3 of the present embodiment, need dropping 1 ~ 2 ether, be about 0.05 ~ 0.1 ml.In step 5), described collection basket is used for depositing slide, when collecting basket and filling, changes empty collection basket.
As preferably, in step 4), describedly being taken off by the lens wiping paper bar covering slide upper surface, is hauled out the side of lens wiping paper from slide.So more be conducive to cleaning oil stain.
embodiment 2:
The present embodiment provides a kind of microslide automatic cleaning apparatus realizing method described in embodiment 1, comprises and supports and transmit the conveyer belt 1 of microslide, conveyer belt stepper motor, transfer system, paper pickup system and cleaning solution Dropping feeder.
Described conveyer belt 1 passes through driving stepper motor.In the present embodiment, conveyer belt 1 is common Industrial conveyor belts, for slide is sent to the other end (end) from one end (initiating terminal).In the present embodiment, the width of conveyer belt 1 is greater than the length of slide.Transfer system, cleaning solution Dropping feeder and paper pickup system is arranged successively from the initiating terminal of conveyer belt 1 to end.Transfer system and paper pickup system are arranged on the both sides of conveyer belt 1, but not in the same side.
Described transfer system is arranged on the side of conveyer belt 1, and described transfer system comprises paper breaking mechanism 4 and ADF 3.
Described ADF 3 is equipped with bar shaped paper tape, in order to be sent by bar shaped paper tape.In the present embodiment, the structure of ADF 3 is comparatively simple, namely comprises an openable housing, described housing has an outlet of stretching out for bar shaped paper tape.Have a cylinder roller in described housing, described paper strip band twists on roller, is similar to the structure of roll web.Described in the present embodiment, bar shaped paper tape is the paper tape that lens wiping paper is made.When described roller rotates, one end of bar shaped paper tape is stretched out from described exit.Further, on described housing, exit slot is higher than the upper surface of conveyer belt 1, is preferably 1 ~ 5cm, so that one end of bar shaped paper tape rides over the upper surface of (namely covering) conveyer belt 1 after stretching out.Further, the lateral edges of conveyer belt 1 is stretched out in the termination of described bar shaped paper tape.
Described paper breaking mechanism 4 comprises the paper knife and chopping block that cooperatively interact, when described paper knife moves downward, can cut off the paper tape between paper knife and chopping block.After described bar shaped paper tape is sent by ADF 3, first through the gap described paper knife and chopping block, then cover the upper surface of conveyer belt 1.As preferably, the edge 1 ~ 5cm of conveyer belt 1 will be stretched out in one end (i.e. free end) of bar shaped paper tape.As shown in Figure 1, the right side that paper breaking mechanism 4 is arranged in the left side of conveyer belt 2, pinch roll is arranged in conveyer belt 2, then, when a part for bar shaped paper tape covers the upper surface of conveyer belt 2, its one end (i.e. free end) stretches out the right side edge 1 ~ 5cm of conveyer belt 1.When slide is sent to (i.e. described primary importance) below bar shaped paper tape by described conveyer belt 1, conveyer belt 1 pauses, and bar shaped paper tape cuts off by described paper knife, makes the one section of bar shaped paper tape be cut off cover the upper surface of slide.
Described cleaning solution Dropping feeder is positioned at above conveyer belt 1.In embodiment, cleaning solution Dropping feeder comprises a cavity built with ether, also has a dropper 2.The outlet of described dropper 2 is positioned at the top of conveyer belt 1.Below the dropper 2 that the slide covering bar shaped paper tape is sent to cleaning solution Dropping feeder time (i.e. the described second place), conveyer belt 1 pauses, and drips ether by described dropper 2 to bar shaped paper tape.
What described paper pickup system comprised the side being arranged on conveyer belt 1 pinches rod, when slide be sent to described pinch the concordant position of rod (i.e. described 3rd position) time, one section of bar shaped paper tape that described slide covers is clamped rear hauling-out by the described rod that pinches.
In the present embodiment, described in pinch rod and comprise installing rack 5 and upper roller 6, and be positioned at the lower roller 7 below described upper roller 6.The axis being parallel of described upper roller 6 and lower roller 7 is in the direction of motion of conveyer belt 1.During use, in order to realize the function of pinch roll, the direction of rotation of upper roller 6 and lower roller 7 is contrary, and the initiating terminal terminad direction namely standing in conveyer belt 2 is seen, the upper roller 6 be positioned on the right side of conveyer belt 2 is rotated counterclockwise, lower roller 7 turns clockwise.
In embodiment, described upper roller 6 and lower roller 7 are cylindrical tube, and its head and the tail two ends are respectively cylindrical upper bottom surface.It should be noted that, the direction of advance of conveyer belt 1 is a horizontal direction, namely points to the direction of its end from the initiating terminal of conveyer belt 1.After upper roller 6 and lower roller 7 install, the direction of advance of conveyer belt 1, breech face are to the direction of advance of conveyer belt 1 dorsad for the head end of described upper roller 6 and lower roller 7, and namely described head end is towards the initiating terminal of conveyer belt 1, and described breech face is to the end of conveyer belt 1.The tail end of described upper roller 6 and lower roller 7 is connected on installing rack 5, and upper roller 6 is not connected with miscellaneous part with the head end of lower roller 7.Further, described installing rack 5 is provided with the rotating shaft that two are parallel to conveyer belt 1 upper surface, the tail end of described upper roller 6 and lower roller 7 is arranged in these two rotating shafts, makes.Rotate under described rotating shaft drives as preferred upper roller 6 and lower roller 7, between described upper roller 6 and lower roller 7, there is gap.
As preferably, the head end of described upper roller 6 and lower roller 7 is nose cone, namely make the gap inlet between upper roller 6 and lower roller 7 have wider space, be convenient to the paper tape be covered on slide and be involved in gap between upper roller 6 and lower roller 7, and then depart from slide.
As preferably, the gap width between described upper roller 6 and lower roller 7 is 0.1 ~ 2mm, is more preferably 0.9mm.Further, upper roller 6 and lower roller 7 are cylinders of two large mini systems, and this cylindrical member at one end is processed to conehead.In embodiment, the gap between described upper roller 6 and lower roller 7 is concordant with the upper surface of conveyer belt 1, and the distance between this gap (i.e. the axis of upper roller 6 and lower roller 7) and conveyer belt 1 edge is preferably 1 ~ 10cm, more preferably 2 ~ 3cm.As mentioned before, the lateral edges of conveyer belt 1 is stretched out in termination due to described bar shaped paper tape, after bar shaped paper tape cuts off by described paper knife, when one section of cut-off bar shaped paper tape covers the upper surface of slide, the length of this section of bar shaped paper tape is greater than the width of conveyer belt 1.Namely the edge 1 ~ 10cm of conveyer belt 1 is stretched out in one end of bar shaped paper tape, in the process of slide along with conveyer belt 1 movement, one end that described bar shaped paper tape stretches out the edge of conveyer belt 1 enters the gap between upper roller 6 and lower roller 7, rolled out by the upper roller 6 that rotates and lower roller 7, the bar shaped paper tape namely made by lens wiping paper by external force is hauled out from the side of slide.Further, described upper roller 6 and be provided with safety screen between lower roller 7 and conveyer belt 1, the upper end of described safety screen exceeds 1 ~ 5mm than the upper surface of conveyer belt 1, and when being rolled out to prevent paper strip band, slide is hauled out.
Further, in use, only need initiating terminal upper surface slide being placed on conveyer belt 2, operation afterwards can realize automation to this device, without the need to manual intervention.Namely at described primary importance, second position installation detecting device, in order to whether to monitor slide by this position.When monitoring slide and arriving primary importance and the second place, conveyer belt 2 all pauses certain hour, as 1 ~ 60s, in order to perform disconnected paper or to drip the operations such as ether.And slide is when described 3rd position, conveyer belt can not stop, and pinch roll also can roll in the whole course of work always, without the need to extra control.To sum up, because the automaticity of this device is higher, in this device outside, a cloche can be installed, the initiating terminal of conveyer belt 2 and end is only made to stay the outside of cloche, like this with regard to making the use procedure of ether occur in relatively airtight environment, effectively reducing it and evaporateing in working environment.Further, at the gateway place of cloche, namely cloche arranges air curtain near the initiating terminal of conveyer belt 2 and the place of end, further prevents ether to evaporate into working environment.

Claims (2)

1. a clean method for histochemical test slide, is characterized in that, comprises the following steps:
1) slide is positioned over the initiating terminal of conveyer belt, the upper surface of described slide speckles with cedar oil;
2) slide is sent to primary importance by described conveyer belt, and pauses; Lens wiping paper is covered at described slide upper surface; The area of described lens wiping paper bar is greater than the area of slide upper surface;
3) slide is sent to the second place by described conveyer belt, and pauses; 0.05 ~ 0.1 mL ether is dripped to the lens wiping paper covering slide upper surface;
4), when slide is sent to the 3rd position by described conveyer belt, the lens wiping paper covering slide upper surface is taken off;
5) described conveyer belt continues to transmit slide, and slide falls into the collection basket be arranged in below conveyer belt end.
2. one kind for realizing the device of said method.
CN201510124817.4A 2013-07-16 2013-07-16 A kind of clean method of histochemical test glass slide Expired - Fee Related CN104785485B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510124817.4A CN104785485B (en) 2013-07-16 2013-07-16 A kind of clean method of histochemical test glass slide

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201510124817.4A CN104785485B (en) 2013-07-16 2013-07-16 A kind of clean method of histochemical test glass slide
CN201310296114.0A CN103331283B (en) 2013-07-16 2013-07-16 Automatic microscope glass slide cleaning method and automatic microscope glass slide cleaning device

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201310296114.0A Division CN103331283B (en) 2013-07-16 2013-07-16 Automatic microscope glass slide cleaning method and automatic microscope glass slide cleaning device

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Publication Number Publication Date
CN104785485A true CN104785485A (en) 2015-07-22
CN104785485B CN104785485B (en) 2018-09-11

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Family Applications (6)

Application Number Title Priority Date Filing Date
CN201310296114.0A Expired - Fee Related CN103331283B (en) 2013-07-16 2013-07-16 Automatic microscope glass slide cleaning method and automatic microscope glass slide cleaning device
CN201510124820.6A Expired - Fee Related CN104785486B (en) 2013-07-16 2013-07-16 A kind of clean method for the microscopic slide speckling with arborvitae oil stain
CN201510124817.4A Expired - Fee Related CN104785485B (en) 2013-07-16 2013-07-16 A kind of clean method of histochemical test glass slide
CN201510124803.2A Expired - Fee Related CN104785484B (en) 2013-07-16 2013-07-16 A kind of microslide cleaning device for preventing ether from volatilizing
CN201510125198.0A Expired - Fee Related CN104772304B (en) 2013-07-16 2013-07-16 A kind of full-automatic laboratory slide cleaning device
CN201510124217.8A Expired - Fee Related CN104785483B (en) 2013-07-16 2013-07-16 A kind of pulley type glass slide cleaning device

Family Applications Before (2)

Application Number Title Priority Date Filing Date
CN201310296114.0A Expired - Fee Related CN103331283B (en) 2013-07-16 2013-07-16 Automatic microscope glass slide cleaning method and automatic microscope glass slide cleaning device
CN201510124820.6A Expired - Fee Related CN104785486B (en) 2013-07-16 2013-07-16 A kind of clean method for the microscopic slide speckling with arborvitae oil stain

Family Applications After (3)

Application Number Title Priority Date Filing Date
CN201510124803.2A Expired - Fee Related CN104785484B (en) 2013-07-16 2013-07-16 A kind of microslide cleaning device for preventing ether from volatilizing
CN201510125198.0A Expired - Fee Related CN104772304B (en) 2013-07-16 2013-07-16 A kind of full-automatic laboratory slide cleaning device
CN201510124217.8A Expired - Fee Related CN104785483B (en) 2013-07-16 2013-07-16 A kind of pulley type glass slide cleaning device

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CN103966020A (en) * 2014-04-29 2014-08-06 周凡 Method for deoiling cedar oil
CN107052747B (en) * 2017-04-17 2018-06-05 鲁曦 A kind of assembling device of Medical glass slide
CN107603868A (en) * 2017-10-25 2018-01-19 芜湖市纯至网络科技有限公司 A kind of biotechnology gene assaying device
CN110327044B (en) * 2019-07-23 2021-09-10 重庆医药高等专科学校 Device for measuring lung ventilation volume
CN113600397A (en) * 2021-07-07 2021-11-05 河北蓝鸟家具股份有限公司 Automatic paint spraying equipment and method for plate-type furniture
CN113959815B (en) * 2021-11-08 2023-08-08 重庆医药高等专科学校 Cell staining device

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JPH04290943A (en) * 1991-03-19 1992-10-15 Hitachi Ltd Automatic specimen preparing device
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Also Published As

Publication number Publication date
CN103331283B (en) 2015-04-15
CN104785483A (en) 2015-07-22
CN104785484B (en) 2018-05-01
CN104785485B (en) 2018-09-11
CN104785486A (en) 2015-07-22
CN104785486B (en) 2018-09-11
CN104772304A (en) 2015-07-15
CN104785484A (en) 2015-07-22
CN104785483B (en) 2018-05-01
CN104772304B (en) 2018-05-08
CN103331283A (en) 2013-10-02

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