CN103966020A - Method for deoiling cedar oil - Google Patents
Method for deoiling cedar oil Download PDFInfo
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- CN103966020A CN103966020A CN201410174993.4A CN201410174993A CN103966020A CN 103966020 A CN103966020 A CN 103966020A CN 201410174993 A CN201410174993 A CN 201410174993A CN 103966020 A CN103966020 A CN 103966020A
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- ether
- oiling
- cedar oil
- ether alcohol
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Abstract
The invention discloses a method for deoiling cedar oil. The method adopts an ether alcohol mixed liquid as a deoiling agent, wherein the ether alcohol mixed liquid is formed by mixing diethyl ether with absolute ethyl alcohol according to the proportion of 70:30; the method comprises the following steps: covering the glass sheet dripped with the cedar oil with absorbing paper with the same size as a glass sheet, absorbing 1 to 2 drips of the ether alcohol mixed liquid mixed formed by mixing diethyl ether with absolute ethyl alcohol according to the proportion of 70:30 through a rubber pipet, and dripping in the center of the glass sheet; picking up the glass sheet through a thumb and an index finger of a left hand, and drawing the absorbing paper rightwards by a right hand at constant speed; putting the glass sheet on which the absorbing paper is removed on a desktop to air, packing, and placing at a shade place to be preserved. According to the method, the deoiling effect is good, karyocyte in a bone marrow piece has no crack after multiple times of deoiling, long-stem storage of the bone marrow piece is facilitated, the operation process is simple, the practicability is strong, and the popularization and application values are higher.
Description
Technical field
The invention belongs to cedar oil de-oiling processing technology field, relate in particular to a kind of method that cedar oil de-oiling is processed.
Background technology
Dimethylbenzene is mainly manufactured in petrochemical process by crude oil, is a kind of water white transparency solvent that is widely used in agricultural chemicals, medicine and explosive, has extremely smellyly, inflammable, can mix arbitrarily insoluble feature in water with ethanol, chloroform and ether.Dimethylbenzene has moderate toxicity, the dimethylbenzene that concentration is higher has obvious pungency damage to cornea, sensitive subjects can be because of xylene pollution skin, cause contact dermatitis, cause skin erythema, fash, split etc., and damage digestive tube, respiratory tract, hemopoietic system, reproductive system etc., even if Low Level Exposure dimethylbenzene, also can there is the neurasthenia symptoms such as dizziness, headache, weak, insomnia, dreaminess and hypomnesis in groups of people.Dimethylbenzene lasts for a long time for the de-cedar oils such as marrow sheet, blood sheet and the clean eyeglass being dyed by cedar oil trowel used for plastering, and staff's health has been caused to damage to a certain degree.
Summary of the invention
The invention provides the novel method that a kind of cedar oil de-oiling is processed, while being intended to avoid adopt at present dimethylbenzene to carry out cedar oil de-oiling processing, the neurasthenia symptoms such as the dizziness that staff is caused, headache, weak, insomnia, dreaminess and hypomnesis, the impact of contact dermatitis and hemopoietic system, respiratory system, Digestive tract etc.
The method that a kind of cedar oil de-oiling provided by the invention is processed, the method that this cedar oil de-oiling is processed adopts ether alcohol mixed solution as de-oiling agent, comprises the following steps:
Step 1, the suction paper with sheet glass size is covered to drip to be had on the sheet glass of cedar oil;
Step 2, inhales 1～2 70: 30 ether alcohol mixing drop Yu Xizhi center with rubber suction pipe;
Step 3, left hand thumb and forefinger are picked up sheet glass, and the right hand at the uniform velocity pulls suction paper to the right;
Step 4, is put in desktop by the sheet glass of taking away suction paper and dries, and mounted box is put shady and cool place and preserved.
Further, by ether and dehydrated alcohol, the ratio according to 70: 30 mixes described ether alcohol mixed solution.
Further, the preparation method of ether alcohol de-oiling agent comprises the following steps:
Step 1, measures the ether of 70 milliliter 100% with the graduated cylinders of 100 milliliters, pour in lucifuge Glass Containers;
Step 2, measures the ethanol of 30 milliliter 100% with the graduated cylinders of 50 milliliters, pour in the lucifuge Glass Containers that ether is housed;
Step 3, stirs ether alcohol mixed solution 3 minutes in the direction of the clock, at the uniform velocity repeatedly with Glass tubing, fully mixes;
Step 4, the ether alcohol mixed solution cover glass configuring lid, In Shade standby.
The method that cedar oil de-oiling provided by the invention is processed, the method adopts ether alcohol mixed solution as de-oiling agent, and by ether and dehydrated alcohol, the ratio according to 70: 30 mixes ether alcohol mixed solution; While avoiding adopting at present dimethylbenzene to carry out cedar oil de-oiling processing, the neurasthenia symptoms such as the dizziness that staff is caused, headache, weak, insomnia, dreaminess and hypomnesis, the impact of contact dermatitis and hemopoietic system, respiratory system, Digestive tract etc.De-oiling is effective, and repeatedly, after de-oiling, the karyocyte flawless in marrow sheet, is applicable to the long-term preservation of marrow sheet, and operating procedure is simple, practical, has stronger propagation and employment and is worth.
Accompanying drawing explanation
Fig. 1 is the schema of the method processed of the cedar oil de-oiling that provides of the embodiment of the present invention.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
As shown in Figure 1, the method that the cedar oil de-oiling of the embodiment of the present invention is processed comprises the following steps:
S101: the suction paper with sheet glass size is covered to drip to be had on the sheet glass of cedar oil;
S102: inhale 1～2 70: 30 ether alcohol mixing drop Yu Xizhi center with rubber suction pipe;
S103: left hand thumb and forefinger are picked up sheet glass, the right hand at the uniform velocity pulls suction paper to the right;
S104: the sheet glass of taking away suction paper is put in to desktop and dries, mounted box, puts shady and cool place and preserve.
By ether and dehydrated alcohol, the ratio according to 70: 30 mixes ether alcohol mixed solution of the present invention; The preparation method of ether alcohol de-oiling agent comprises the following steps:
Step 1, measures the ether of 70 milliliter 100% with the graduated cylinders of 100 milliliters, pour in lucifuge Glass Containers;
Step 2, measures the ethanol of 30 milliliter 100% with the graduated cylinders of 50 milliliters, pour in the lucifuge Glass Containers that ether is housed;
Step 3, stirs ether alcohol mixed solution 3 minutes in the direction of the clock, at the uniform velocity repeatedly with Glass tubing, fully mixes;
Step 4, the ether alcohol mixed solution cover glass configuring lid, In Shade standby.
Below application principle of the present invention is further described.
Materials and methods:
1.1 cases and grouping 30 examples (male 14 examples, female's 16 examples), 25～76 years old age, average 43 years old.Myelodysplastic syndrome 4 examples in 30 examples, polycythemia vera 1 example, Hodgkin lymphoma 1 example, multiple myeloma 4 examples, aplastic anemia 1 example, myelofibrosis 2 examples, liver cirrhosis 1 example, fever of unknown 4 examples, acute lymphoblastic leukemia 2 examples, acute nonlymphocytic leukemia 8 examples, chronic myelocytic leukemia 2 examples.30 examples are totally 570 routine marrow sheets, and every example is retained 19 of bone marrow smears, is divided into 19 groups, 30 every group (1 of every example).
1.2 preparations are prepared a certain proportion of ether alcohol mixed solution on demand, and its ether/alcohol ratio is 0/100,20/80,25/75,30/70,35/65,40/60,50/50,60/40,70/30,80/20,100/0.Other also has dimethylbenzene, staining for glycogen reagent, an auspicious Ji Shi dye liquor.
1.3 concrete grammars are divided into following 5 kinds of methods to be processed.
(1) wherein 11 groups of marrow sheet (30 every group) every days or the next day get 1 group of marrow sheet, with after an auspicious Ji Shi dye liquor dyeing, at oily Microscopic observation bone marrow smear, observe karyocyte and whether have slight crack, then use the ether alcohol mixed solution de-oiling (cedar oil) of a kind of concentration, be followed in the 1st, 5,24,48 hours, in the time of 1 week, 2 weeks, 1 month, in oily Microscopic observation cell cracks, after observation, all use the ether alcohol mixed solution de-oiling of this concentration.In each record 30 examples, cell has the umber of crackle, and counts 100 karyocytes, records the percentage of crackle cell, calculates the mean percentage and the severe crack degree that there are crackle cell.
(2) get 1 group of marrow sheet and dye as stated above, observe, but use dimethylbenzene de-oiling.
(3) get 3 groups of marrow sheets, after the dyeing of Rui-Ji Shi dye liquor, drip cedar oil, at oily Microscopic observation, with 60/40,70/30,80/20 ether alcohol mixed solution is separately after de-oiling, not interval time, continuous oily sem observation, de-oiling, totally 6 times, the crackle umber of observation of cell, crackle cell mean percentage and severity thereof.
(4) get 1 group of marrow sheet, with dehydrated alcohol, fix, carry out staining for glycogen, observation of cell cracks.
(5) get 3 groups of marrow sheets, after the dyeing of Rui-Ji Shi dye liquor, drip cedar oil, oily sem observation, after 60/40,70/30,80/20 ether alcohol mixed solution de-oiling 1 time, interval is observed for 1 month.
1.4 crackle deciding degree standards divide gently, in, weigh 3 grades.Slight: in karyocyte, only to have 1 Crack, only on endochylema, split and do not involve core; Moderate: split 1, cross core (being that endochylema and core all have crackle), or split 2 (involve core or do not involve core); Severe: 1 karyocyte has 3 above crackles (involve core or do not involve core).
In 1 marrow sheet, there is the degree weight of crackle cell to differ, by there being the born of the same parents' line number in the cell of crackle to be added, divided by the cell count that has crackle, draw on average causing of crackle, by above standard determination.
2 results
2.130 example auspicious-Giemsa stain after, in oily Microscopic observation, 29 routine cells are complete, flawless, 1 routine cell has crackle.
After 2.2 use ether alcohol mixed solution 0/100,20/80 de-oilings, almost every part of marrow sheet is all shown in the karyocyte that has crackle, counts 100 karyocytes, have the karyocyte mean percentage of crackle higher, and crackle degree mostly is severe; And after 25/75,80/70,35/65,40/60,50/50 de-oiling of ether alcohol mixed solution, thering is the number of cases of crackle cell, the mean percentage of crackle cell is lower, its crackle degree is medium degree; Ether alcohol mixed solution is 60/40,70/30,80/20 o'clock, has the number of cases of crackle cell, crackle cell average percent very low, and is slight;
2.3 with in 0/100,20/80,25/75,30/70,35/65,40/60,50/50 ether alcohol mixing slag de-oiling marrow sheet, there is the number of cases of crackle cell, the mean percentage of crackle cell and Crack Strength all increase with the increase of de-oiling number of times, and 60/40, the mixed platform of 70/30,80/20 ether alcohol covers in de-oiling marrow sheet without this phenomenon.
2.4 not time, oily sem observation aldehyde alcohol mixed solution (60/40,70/30,80/20) de-oilings repeatedly continuously at regular intervals, cell flawless still after visible de-3 oil, after de-4 times, there is cell crackle, illustrate that de-oiling number of times increases, cell occurs that the chance of crackle increases, but at the marrow sheet of the ether alcohol mixed solution de-oiling of these 3 concentration, after 4 de-oilings, the number of cases with crackle cell is only 2～3 examples, and the mean percentage of its crackle cell only accounts for 0.5%～2%.
The marrow sheet of 2.5 use dimethylbenzene de-oilings, the equal flawless of cell.
2.6 use dehydrated alcohols are marrow sheet fixedly, carries out staining for glycogen, and in marrow sheet, cell has no crackle.
2.7 with the marrow sheet of alcohol ether liquid de-oiling, it has the number of cases of crackle cell and the mean percentage of crackle cell and 80/20 ether alcohol mixed solution roughly the same, its degree is also unanimous on the whole, but too fast because volatilizing, and de-oiling weak effect should not be used.
2.8 use ether alcohol mixing slag de-oilings, on lens wiping paper, have slightly painted, but on the color and luster of bone marrow smear without impact, even repeatedly de-oiling, also without impact;
2.9 there is the marrow sheet of cell crackle, irrelevant with kinds of Diseases and cell category.
After 2.10 different ether alcohol mixed solution de-oilings 1 time, de-oiling person repeatedly not, in 60/40,70/30, the ether alcohol mixed solution of 80/20 ratio.Only de-1 oily marrow is preserved sheet, preserve cell flawless phenomenon after 1 month, and the ether alcohol mixed solution of other concentration has the crackle cell of varying degree as seen.
3 find out from test-results, ether alcohol mixed solution is operable as cedar oil de-oilings such as bone marrow smear, blood sheets, 60/40, the ether alcohol mixed solution of 70/30,80/20 ratio all can be used, but the ether alcohol mixed solution of 70/30 ratio of take is optimum, not only de-oiling is thorough. and marrow sheet is clean, fast drying, after de-oiling 1～3 time, still flawless generation of cell in marrow sheet, though visible indivedual cells have unconspicuous slight crackle in the indivedual marrow sheets of repetitious de-oiling, but do not affect, do not read sheet and preservation, and file after generally marrow sheet is read sheet 1 time, repeatedly do not read sheet.Though pure ether is dry fast, therefore de-oiling is unclean, therefore should not use.
With the cell crackle after the de-oiling of ether alcohol mixed solution, with certainly to choose crackle different, the former crackle is neat, crackle clear-cut margin, and severe patient is as polygon, and the aging crack of cell irregular sharp keen, easily difference.
Ether alcohol mixed solution is volatile, therefore need pack in the clear bottle of ground, once preparation is not too many for ether alcohol mixed solution.
The marrow sheet that dehydrated alcohol is fixing, carries out after cytochemical staining, and in this smear, having no cell has seminess; And after the dyeing of Rui-Ji dye liquor, oily sem observation, during with dehydrated alcohol de-oiling, obviously, above-mentioned phenomenon mechanism is unclear in cell be full of cracks.
The method that cedar oil de-oiling that the embodiment of the present invention provides is processed, the method adopts ether alcohol mixed solution as de-oiling agent, and by ether and dehydrated alcohol, the ratio according to 70: 30 mixes ether alcohol mixed solution; De-oiling is effective, and repeatedly, after de-oiling, the karyocyte flawless in marrow sheet, is applicable to the long-term preservation of marrow sheet, and operating procedure is simple, practical, has stronger propagation and employment and is worth.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (3)
1. the method that cedar oil de-oiling is processed, is characterized in that, the method that this cedar oil de-oiling is processed adopts ether alcohol mixed solution as de-oiling agent, comprises the following steps:
Step 1, the suction paper with sheet glass size is covered to drip to be had on the sheet glass of cedar oil;
Step 2, inhales 1～2 70: 30 ether alcohol mixing drop Yu Xizhi center with rubber suction pipe;
Step 3, left hand thumb and forefinger are picked up sheet glass, and the right hand at the uniform velocity pulls suction paper to the right;
Step 4, is put in desktop by the sheet glass of taking away suction paper and dries, and mounted box is put shady and cool place and preserved.
2. the method that cedar oil de-oiling as claimed in claim 1 is processed, is characterized in that, by ether and dehydrated alcohol, the ratio according to 70: 30 mixes described ether alcohol mixed solution.
3. the method that cedar oil de-oiling as claimed in claim 1 is processed, is characterized in that, the preparation method of ether alcohol de-oiling agent comprises the following steps:
Step 1, measures the ether of 70 milliliter 100% with the graduated cylinders of 100 milliliters, pour in lucifuge Glass Containers;
Step 2, measures the ethanol of 30 milliliter 100% with the graduated cylinders of 50 milliliters, pour in the lucifuge Glass Containers that ether is housed;
Step 3, stirs ether alcohol mixed solution 3 minutes in the direction of the clock, at the uniform velocity repeatedly with Glass tubing, fully mixes;
Step 4, the ether alcohol mixed solution cover glass configuring lid, In Shade standby.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410174993.4A CN103966020A (en) | 2014-04-29 | 2014-04-29 | Method for deoiling cedar oil |
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| CN201410174993.4A CN103966020A (en) | 2014-04-29 | 2014-04-29 | Method for deoiling cedar oil |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108896363A (en) * | 2018-05-28 | 2018-11-27 | 杭州智微信息科技有限公司 | A kind of digitized flow and method of bone marrow smear |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103331283A (en) * | 2013-07-16 | 2013-10-02 | 重庆医药高等专科学校 | Automatic microscope glass slide cleaning method and automatic microscope glass slide cleaning device |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103331283A (en) * | 2013-07-16 | 2013-10-02 | 重庆医药高等专科学校 | Automatic microscope glass slide cleaning method and automatic microscope glass slide cleaning device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108896363A (en) * | 2018-05-28 | 2018-11-27 | 杭州智微信息科技有限公司 | A kind of digitized flow and method of bone marrow smear |
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Application publication date: 20140806 |