CN104785485B - A kind of clean method of histochemical test glass slide - Google Patents

A kind of clean method of histochemical test glass slide Download PDF

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Publication number
CN104785485B
CN104785485B CN201510124817.4A CN201510124817A CN104785485B CN 104785485 B CN104785485 B CN 104785485B CN 201510124817 A CN201510124817 A CN 201510124817A CN 104785485 B CN104785485 B CN 104785485B
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CN
China
Prior art keywords
glass slide
conveyer belt
paper
roller
covered
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201510124817.4A
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Chinese (zh)
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CN104785485A (en
Inventor
刘怡
景戌
王惠
邱妍川
韦丽佳
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Chongqing Medical and Pharmaceutical College
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Chongqing Medical and Pharmaceutical College
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Publication date
Application filed by Chongqing Medical and Pharmaceutical College filed Critical Chongqing Medical and Pharmaceutical College
Priority to CN201510124817.4A priority Critical patent/CN104785485B/en
Publication of CN104785485A publication Critical patent/CN104785485A/en
Application granted granted Critical
Publication of CN104785485B publication Critical patent/CN104785485B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B11/00Cleaning flexible or delicate articles by methods or apparatus specially adapted thereto
    • B08B11/04Cleaning flexible or delicate articles by methods or apparatus specially adapted thereto specially adapted for plate glass, e.g. prior to manufacture of windshields
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B1/00Cleaning by methods involving the use of tools
    • B08B1/10Cleaning by methods involving the use of tools characterised by the type of cleaning tool
    • B08B1/14Wipes; Absorbent members, e.g. swabs or sponges
    • B08B1/143Wipes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04Cleaning involving contact with liquid
    • B08B3/08Cleaning involving contact with liquid the liquid having chemical or dissolving effect
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B7/00Cleaning by methods not provided for in a single other subclass or a single group in this subclass

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cleaning In General (AREA)
  • Re-Forming, After-Treatment, Cutting And Transporting Of Glass Products (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention discloses a kind of clean method of histochemical test glass slide, clean method includes:Glass slide is positioned over to the initiating terminal of conveyer belt, the upper surface of the glass slide speckles with cedar oil;Glass slide is transmitted to first position by the conveyer belt, and is paused;Lens wiping paper is covered in the glass slide upper surface;Glass slide is transmitted to the second position by the conveyer belt, and is paused;Ether is added dropwise to the lens wiping paper for being covered in glass slide upper surface;When glass slide is transmitted to the third place by the conveyer belt, the lens wiping paper for being covered in glass slide upper surface is removed;The conveyer belt continues to transmit glass slide, and glass slide is fallen into the collection basket below transmission end of tape;Microscopic slide automatic cleaning apparatus includes supporting and transmitting the conveyer belt of microscopic slide, conveyer belt stepper motor, transfer system, paper pickup system and cleaning solution Dropping feeder, it improves work efficiency, reduce contact of the staff with harmful substance, brings significant benefit.

Description

A kind of clean method of histochemical test glass slide
The present patent application be the applying date be on 07 16th, 2013, application No. is 201310296114.0 divisional applications.
Technical field
The present invention relates to experimental instrument fields, are specifically a kind of microscopic slide clean method and device.
Background technology
It is biochemical real in conventional histology, histochemistry and histiocytic immunohistochemistry, fluorescent staining, in situ hybridization etc. In testing, glass slide is the important carrier of sample, and the glass slide for microscope oil mirror diagosis needs that cedar oil is added dropwise to change folding Rate is penetrated to improve picture quality.
Glass slide after use is needed because its upper surface speckles with arborvitae oil stain with ether or natalite or diformazan The reagents such as benzene are dissolved.Otherwise, the oil stain being set on glass slide after drying can seriously affect the picture quality of next diagosis. But there is the serious harm to human body in the reagents such as ether.
Currently, the cleaning for speckling with the glass slide of cedar oil, which relies primarily on laboratory worker, is added dropwise reagent in glass slide On, cedar oil and then wiped clean are made it dissolve, working efficiency is low, and laboratory worker contacts nuisance for a long time Matter, there are serious security risks.Therefore it provides one kind is efficient, avoids laboratory worker contact nuisance for a long time The microscopic slide cleaning solution of matter be just particularly important with it is urgent.
Invention content
An object of the present invention is to provide a kind of microscopic slide clean method that cleaning efficiency is high.
To realize the present invention purpose and the technical solution adopted is that a kind of such, microscopic slide automated cleaning side Method includes the following steps:
1) glass slide is positioned over to the initiating terminal of conveyer belt, the upper surface of the glass slide speckles with cedar oil.
2) glass slide is transmitted to first position by the conveyer belt, and is paused.It is covered in the glass slide upper surface and wipes mirror Paper.
3) glass slide is transmitted to the second position by the conveyer belt, and is paused.To the wiping mirror for being covered in glass slide upper surface Ether is added dropwise in paper.
4) when glass slide is transmitted to the third place by the conveyer belt, the lens wiping paper item for being covered in glass slide upper surface is taken Under.
5) conveyer belt continues to transmit glass slide, and glass slide is fallen into the collection basket below transmission end of tape.
In the method for the present invention, it is only necessary to ether equal solvent marginally is added dropwise to the lens wiping paper being covered on glass slide, and After ether dissolution cedar oil oil stain, the mixture of oil stain and ether is present in lens wiping paper, it is only necessary to handle lens wiping paper well, just not It can cause a large amount of ether volatilization in air.
It is a further object to provide a kind of microscopic slide automatic cleaning apparatus for realizing the above method, should Device includes supporting and transmitting the conveyer belt of microscopic slide, conveyer belt stepper motor, transfer system, paper pickup system and cleaning Drop feeder apparatus.The conveyer belt is driven by stepper motor, and the cleaning solution Dropping feeder is located above conveyer belt.It is described to pass Paper system is mounted on the side of conveyer belt, and the transfer system includes paper breaking mechanism and feeder.The feeder is equipped with bar shaped Paper tape, the paper breaking mechanism include mutually matched paper knife and chopping block.After the paper strip band is sent out by feeder, initially pass through Gap between the paper knife and chopping block, then it is covered in the upper surface of conveyer belt.When glass slide is transmitted to by the conveyer belt When below paper strip band, the paper knife cuts off paper strip band so that one section of cut-off paper strip band is covered in glass slide Upper surface.Ether is housed, when the glass slide for covering paper strip band is sent to cleaning solution in the cleaning solution Dropping feeder When below the dropper of Dropping feeder, ether is added dropwise to paper strip band by the dropper.The paper pickup system includes being mounted on to pass The pinch roller for sending the side of band is covered when glass slide is sent to the position concordant with the pinch roller on the glass slide One section of paper strip band clamped by the pinch roller after haul out.
Having the technical effect that for the device of the invention is unquestionable, is added dropwise as a result of automatic transfer system, cleaning solution Device and paper pickup system realize the batch cleaning to glass slide, improve work efficiency, reduce staff and nuisance The contact of matter brings significant benefit.Further, the device of the invention can be placed in confined space, more effectively be prevented Ether is evaporate into working environment, reduces work risk.
Description of the drawings
The device of the invention can be further illustrated by the nonlimiting examples that attached drawing provides.
Fig. 1 is schematic structural view of the invention;
Fig. 2 is the schematic diagram that glass slide is in " first position ";
Fig. 3 is the schematic diagram that glass slide is in " second position ";
Fig. 4 is the schematic diagram that glass slide is in " the third place ".
In figure:1- conveyer belts, 2- droppers, 3- feeder, 4- paper breaking mechanisms, 5- mounting brackets, 6- upper rollers, 7- lower rollers.
Specific implementation mode
The invention will be further described with reference to the accompanying drawings and examples, but should not be construed the above-mentioned theme of the present invention Range is only limitted to following embodiments.Without departing from the idea case in the present invention described above, known according to ordinary skill Knowledge and customary means, make various replacements and change, should all be included in the scope of the present invention.
Embodiment 1:
The present embodiment discloses a kind of microscopic slide automatic cleaning method, includes the following steps:
1) glass slide is positioned over to the initiating terminal of conveyer belt, the upper surface of the glass slide speckles with cedar oil;
2) glass slide is transmitted to first position by the conveyer belt, and is paused;It is covered in the glass slide upper surface and wipes mirror Paper;
3) glass slide is transmitted to the second position by the conveyer belt, and is paused;To the wiping mirror for being covered in glass slide upper surface Ether is added dropwise in paper;
4) when glass slide is transmitted to the third place by the conveyer belt, it can pause or not stop, glass slide will be covered in The lens wiping paper item of upper surface is removed;
5) conveyer belt continues to transmit glass slide, and glass slide is fallen into the collection basket below transmission end of tape.
It is worth noting that in step 2~4 in the present embodiment, the first position, the second position and the third place are Positioned at transmission tape beginning end and end between three nonoverlapping positions, glass slide behind the upper surface for being placed in conveyer belt 1, Pass through these three positions successively." pause " is 1 stop motion of conveyer belt, can pass through step motor control.When load glass When piece is transmitted to these three positions, it is intended to pause, is operated with facilitating.The area of the lens wiping paper item and glass slide upper surface Area it is suitable, but can also be more than glass slide upper surface area.In the step 3 of the present embodiment, dropwise addition 1~2 is needed to drip second Ether, about 0.05~0.1ml.In step 5), the collection basket, when collection basket is filled, replaces sky for storing glass slide Collect basket.
It is described to remove the lens wiping paper item for being covered in glass slide upper surface preferably, in step 4), be by lens wiping paper from It hauls out the side of glass slide.It is more advantageous to the cleaning to oil stain in this way.
Embodiment 2:
The present embodiment provides a kind of microscopic slide automatic cleaning apparatus for realizing 1 the method for embodiment, including branch It supports and dress is added dropwise in conveyer belt 1, conveyer belt stepper motor, transfer system, paper pickup system and the cleaning solution for transmitting microscopic slide It sets.
The conveyer belt 1 is driven by stepper motor.In the present embodiment, the as common Industrial conveyor belts of conveyer belt 1, For glass slide to be transmitted to the other end (end) from one end (initiating terminal).In the present embodiment, the width of conveyer belt 1, which is more than, carries glass The length of piece.Transfer system, cleaning solution Dropping feeder and paper pickup system are sequentially arranged from the initiating terminal of conveyer belt 1 to end.It passs Paper system and paper pickup system are mounted on the both sides of conveyer belt 1, but not in the same side.
The transfer system is mounted on the side of conveyer belt 1, and the transfer system includes paper breaking mechanism 4 and feeder 3.
The feeder 3 is equipped with paper strip band, to send out paper strip band.In the present embodiment, the structure of feeder 3 compared with To be simple, that is, include an openable shell, there are one the outlets stretched out for paper strip band for tool on the shell.The shell For tool there are one cylinder roller, the bar shaped reel is similar to the structure of roll web on roller in vivo.Described in the present embodiment Paper strip band is paper tape made of lens wiping paper.When the roller rotates, one end of paper strip band is stretched out from the exit. Further, upper surface of the exit slot higher than conveyer belt 1 on the shell, it is therefore preferable to 1~5cm, in order to the one of paper strip band End rides over and (is covered in) upper surface of conveyer belt 1 after stretching out.Further, conveyer belt 1 is stretched out in the end of the paper strip band Lateral edges.
The paper breaking mechanism 4 includes mutually matched paper knife and chopping block, when the paper knife moves downward, can be cut off Paper tape between paper knife and chopping block.After the paper strip band is sent out by feeder 3, the paper knife and chopping block are initially passed through Between gap, then be covered in the upper surface of conveyer belt 1.Preferably, one end (i.e. free end) of paper strip band will stretch out biography Send 1~5cm of edge of band 1.As shown in Figure 1, paper breaking mechanism 4 is arranged in the left side of conveyer belt 1, pinch roller is arranged in conveyer belt 1 Right side, then when a part for paper strip band is covered in the upper surface of conveyer belt 1, one end (i.e. free end) stretch out conveyer belt 1 1~5cm of right side edge.When glass slide is transmitted to (the i.e. described first position) below paper strip band by the conveyer belt 1, pass Band 1 is sent to pause, the paper knife cuts off paper strip band so that one section of cut-off paper strip band is covered in the upper table of glass slide Face.
The cleaning solution Dropping feeder is located at 1 top of conveyer belt.In embodiment, cleaning solution Dropping feeder includes one built-in There is the cavity of ether, there are one droppers 2.The outlet of the dropper 2 is positioned at the top of conveyer belt 1.When covering paper strip band Glass slide when being sent to the lower section of dropper 2 (the i.e. described second position) of cleaning solution Dropping feeder, the pause of conveyer belt 1 passes through Ether is added dropwise to paper strip band in the dropper 2.
The paper pickup system includes the pinch roller mounted on the side of conveyer belt 1, when glass slide is sent to and the folder It send when rolling out neat position (the i.e. described the third place), the one section of paper strip band covered on the glass slide is pressed from both sides by the pinch roller It is hauled out after firmly.
In the present embodiment, the pinch roller includes mounting bracket 5 and upper roller 6, and under 6 lower section of the upper roller Roller 7.The axis of the upper roller 6 and lower roller 7 is parallel to the direction of motion of conveyer belt 1.In use, in order to realize pinch roller Function, the direction of rotation of upper roller 6 and lower roller 7 is located on the contrary, i.e. station is seen in the initiating terminal terminad direction of conveyer belt 1 Rotation, lower roller 7 rotate clockwise the upper roller 6 on 1 right side of conveyer belt counterclockwise.
In embodiment, the upper roller 6 and lower roller 7 are cylindrical tube, and head and the tail both ends are respectively the upper of cylinder Bottom surface.It should be noted that the direction of advance of conveyer belt 1 is a horizontal direction, i.e., it is directed toward it from the initiating terminal of conveyer belt 1 The direction of end.After upper roller 6 and lower roller 7 install, the head end of the upper roller 6 and lower roller 7 is backwards to conveyer belt 1 The direction of advance of direction of advance, tail end towards conveyer belt 1, i.e., described initiating terminal of the head end towards conveyer belt 1, the tail end towards The end of conveyer belt 1.The tail end of the upper roller 6 and lower roller 7 is connected on mounting bracket 5, the head of upper roller 6 and lower roller 7 End is not connect with other component.Further, two shafts for being parallel to 1 upper surface of conveyer belt are installed on the mounting bracket 5, The tail end of the upper roller 6 and lower roller 7 is mounted in this two shafts so that.Exist as preferred upper roller 6 and lower roller 7 The lower rotation of shaft driving, has gap between the upper roller 6 and lower roller 7.
Preferably, the head end of the upper roller 6 and lower roller 7 is nose cone, i.e., so that upper roller 6 and lower roller Gap inlet between 7 has wider space, is involved in upper roller 6 and lower roll convenient for being covered in the paper tape on glass slide Gap between cylinder 7, and then it is detached from glass slide.
Preferably, the gap width between the upper roller 6 and lower roller 7 is 0.1~2mm, more preferably 0.9mm.Further, upper roller 6 and lower roller 7 are the cylinders of two big mini system, which is processed to conehead. In embodiment, the gap between the upper roller 6 and lower roller 7 is concordant with the upper surface of conveyer belt 1, and gap (the i.e. upper roller The axis of cylinder 6 and lower roller 7) with the distance between 1 edge of conveyer belt preferably 1~10cm, be more preferably 2~3cm.Such as It is described previously, since the lateral edges of conveyer belt 1 are stretched out in the end of the paper strip band, when the paper knife cuts off paper strip band Afterwards, when one section of cut-off paper strip band is covered in the upper surface of glass slide, the length of this section of paper strip band is greater than conveyer belt 1 Width.I.e. 1~10cm of edge of conveyer belt 1 is stretched out in one end of paper strip band, in the process that glass slide is moved with conveyer belt 1 In, one end that the paper strip band stretches out the edge of conveyer belt 1 enters the gap between upper roller 6 and lower roller 7, is rotated Upper roller 6 and lower roller 7 roll out, i.e., are hauled out paper strip band made of lens wiping paper from the side of glass slide external force.More into One step is equipped with safety screen between the upper roller 6 and lower roller 7 and conveyer belt 1, the upper end of the safety screen is than transmission It is higher by 1~5mm with 1 upper surface, when preventing paper strip band to be unwrapped, glass slide is dragged out.
Further, the present apparatus is in use, it is only necessary to glass slide is placed on to the initiating terminal upper surface of conveyer belt 1, Operation later may be implemented to automate, and be not necessarily to manual intervention.I.e. in the first position, second place installation detection dress It sets, whether to monitor glass slide by the position.When monitoring that glass slide reaches first position and the second position, conveyer belt 1 pauses certain time, such as 1~60s, to execute disconnected paper or the operations such as ether are added dropwise.And glass slide is in the third place When, conveyer belt can not stop, and pinch roller can also always roll in whole work process, without additional control.To sum up, by It is higher in the degree of automation of the present apparatus, a cloche can be installed outside the present apparatus, only so that the initiating terminal of conveyer belt 1 The outside of cloche is stayed in end, allows for being happened at using process in relatively closed environment for ether in this way, effectively It is reduced to evaporate into working environment.Further, at the entrance of cloche, i.e. starting of the cloche close to conveyer belt 1 The place setting air curtain at end and end, further prevents ether to evaporate into working environment.

Claims (1)

1. a kind of clean method of histochemical test glass slide, which is characterized in that the device used is placed in confined space, that is, exists One cloche is installed, only so that the initiating terminal of conveyer belt and end stay in the outside of cloche outside the present apparatus;
The device of use includes:Support and transmit the conveyer belt (1) of microscopic slide, conveyer belt stepper motor, transfer system, Paper pickup system and cleaning solution Dropping feeder;
The conveyer belt (1) is driven by stepper motor, and the cleaning solution Dropping feeder is located above conveyer belt (1);
The transfer system is mounted on the side of conveyer belt (1), and the transfer system includes paper breaking mechanism (4) and feeder (3); The feeder (3) is equipped with paper strip band, and the paper breaking mechanism (4) includes mutually matched paper knife and chopping block;The bar shaped After paper tape is sent out by feeder (3), the gap between the paper knife and chopping block is initially passed through, then be covered in the upper of conveyer belt (1) Surface;When glass slide is transmitted to below paper strip band by the conveyer belt (1), the paper knife cuts off paper strip band, makes The one section of paper strip band that must be cut off is covered in the upper surface of glass slide;
Cleaning solvent is housed, the cleaning solvent is ether, when the load for covering paper strip band in the cleaning solution Dropping feeder When slide is sent to below the dropper (2) of cleaning solution Dropping feeder, ether is added dropwise to paper strip band by the dropper (2);
The paper pickup system includes the pinch roller mounted on the side of conveyer belt (1), when glass slide is sent to and the pinch When rolling out neat position, one section of paper strip band being covered on the glass slide is hauled out after being clamped by the pinch roller;
The pinch roller includes mounting bracket (5) and upper roller (6), and the lower roller (7) below the upper roller (6); The axis of the upper roller (6) and lower roller (7) is parallel to the direction of motion of conveyer belt (1);The upper roller (6) and lower roller (7) direction of advance, tail end direction of advance towards conveyer belt (1) of the head end backwards to conveyer belt (1);The upper roller (6) and The tail end of lower roller (7) is connected on mounting bracket (5);There is gap between the upper roller (6) and lower roller (7);
One cloche is installed, only so that the initiating terminal of conveyer belt (1) and end stay in the outside of cloche outside device;
Include the following steps:
1) glass slide is positioned over to the initiating terminal of conveyer belt, the upper surface of the glass slide speckles with cedar oil;
2) glass slide is transmitted to first position by the conveyer belt, and is paused;Lens wiping paper is covered in the glass slide upper surface;Institute The area for stating lens wiping paper is more than the area of glass slide upper surface;
3) glass slide is transmitted to the second position by the conveyer belt, and is paused;It is dripped to the lens wiping paper for being covered in glass slide upper surface Add 0.05~0.1mL ether;
4) when glass slide is transmitted to the third place by the conveyer belt, the lens wiping paper for being covered in glass slide upper surface is removed;
5) conveyer belt continues to transmit glass slide, and glass slide is fallen into the collection basket below transmission end of tape.
CN201510124817.4A 2013-07-16 2013-07-16 A kind of clean method of histochemical test glass slide Expired - Fee Related CN104785485B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510124817.4A CN104785485B (en) 2013-07-16 2013-07-16 A kind of clean method of histochemical test glass slide

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201310296114.0A CN103331283B (en) 2013-07-16 2013-07-16 Automatic microscope glass slide cleaning method and automatic microscope glass slide cleaning device
CN201510124817.4A CN104785485B (en) 2013-07-16 2013-07-16 A kind of clean method of histochemical test glass slide

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201310296114.0A Division CN103331283B (en) 2013-07-16 2013-07-16 Automatic microscope glass slide cleaning method and automatic microscope glass slide cleaning device

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CN104785485A CN104785485A (en) 2015-07-22
CN104785485B true CN104785485B (en) 2018-09-11

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Application Number Title Priority Date Filing Date
CN201510124820.6A Expired - Fee Related CN104785486B (en) 2013-07-16 2013-07-16 A kind of clean method for the microscopic slide speckling with arborvitae oil stain
CN201510124803.2A Expired - Fee Related CN104785484B (en) 2013-07-16 2013-07-16 A kind of microslide cleaning device for preventing ether from volatilizing
CN201310296114.0A Expired - Fee Related CN103331283B (en) 2013-07-16 2013-07-16 Automatic microscope glass slide cleaning method and automatic microscope glass slide cleaning device
CN201510124217.8A Expired - Fee Related CN104785483B (en) 2013-07-16 2013-07-16 A kind of pulley type glass slide cleaning device
CN201510125198.0A Expired - Fee Related CN104772304B (en) 2013-07-16 2013-07-16 A kind of full-automatic laboratory slide cleaning device
CN201510124817.4A Expired - Fee Related CN104785485B (en) 2013-07-16 2013-07-16 A kind of clean method of histochemical test glass slide

Family Applications Before (5)

Application Number Title Priority Date Filing Date
CN201510124820.6A Expired - Fee Related CN104785486B (en) 2013-07-16 2013-07-16 A kind of clean method for the microscopic slide speckling with arborvitae oil stain
CN201510124803.2A Expired - Fee Related CN104785484B (en) 2013-07-16 2013-07-16 A kind of microslide cleaning device for preventing ether from volatilizing
CN201310296114.0A Expired - Fee Related CN103331283B (en) 2013-07-16 2013-07-16 Automatic microscope glass slide cleaning method and automatic microscope glass slide cleaning device
CN201510124217.8A Expired - Fee Related CN104785483B (en) 2013-07-16 2013-07-16 A kind of pulley type glass slide cleaning device
CN201510125198.0A Expired - Fee Related CN104772304B (en) 2013-07-16 2013-07-16 A kind of full-automatic laboratory slide cleaning device

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CN107603868A (en) * 2017-10-25 2018-01-19 芜湖市纯至网络科技有限公司 A kind of biotechnology gene assaying device
CN110327044B (en) * 2019-07-23 2021-09-10 重庆医药高等专科学校 Device for measuring lung ventilation volume
CN113600397A (en) * 2021-07-07 2021-11-05 河北蓝鸟家具股份有限公司 Automatic paint spraying equipment and method for plate-type furniture
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Publication number Publication date
CN104785485A (en) 2015-07-22
CN104785483A (en) 2015-07-22
CN103331283B (en) 2015-04-15
CN104785483B (en) 2018-05-01
CN104772304B (en) 2018-05-08
CN104772304A (en) 2015-07-15
CN104785486A (en) 2015-07-22
CN103331283A (en) 2013-10-02
CN104785486B (en) 2018-09-11
CN104785484B (en) 2018-05-01
CN104785484A (en) 2015-07-22

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